CN114391656A - Giant salamander oligopeptide health product - Google Patents
Giant salamander oligopeptide health product Download PDFInfo
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- CN114391656A CN114391656A CN202210151257.1A CN202210151257A CN114391656A CN 114391656 A CN114391656 A CN 114391656A CN 202210151257 A CN202210151257 A CN 202210151257A CN 114391656 A CN114391656 A CN 114391656A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/02—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from meat
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention discloses a giant salamander oligopeptide health product. The raw materials comprise the following components in parts by weight: 20-30 parts of giant salamander oligopeptide, 3-5 parts of liquorice extract and 2-4 parts of kudzu root extract. According to the invention, the giant salamander oligopeptide, the licorice extract and the kudzu root extract are compounded as raw materials, so that the bitter taste and the fishy smell of the giant salamander oligopeptide are greatly reduced, the market acceptance is greatly improved, and the market of the giant salamander deep-processed product is expanded.
Description
Technical Field
The invention belongs to the field of health-care products, and particularly relates to a giant salamander oligopeptide health-care product.
Background
Giant salamanders (Giant salamanders) are the most precious amphibians existing in the world, and have high nutritional value and delicious taste. With the continuous expansion of the culture scale and the number of the second generation giant salamanders, the number of giant salamander products is more and more, but at present, the deep processed giant salamander products in the market are still deficient, so the development of the deep processed giant salamander products becomes the bottleneck of the transformation and upgrading of the industry. The development of giant salamander deep-processed products such as giant salamander peptide (research reports that giant salamander peptide has very good effects of resisting oxidation, aging, fatigue and the like) also faces some problems, on one hand, most of the development and application at present mainly take giant salamander meat as a main material, and the report that the whole fish is taken as a raw material to be subjected to a specific enzymolysis process to obtain the peptide with smaller molecular weight and the peptide with specific effect does not exist at present; on the other hand, while the giant salamander peptide is extremely high in nutritional value, it has some bitter and fishy tastes, which reduces the consumer acceptance and limits the market size.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a giant salamander oligopeptide health-care product, which specifically adopts the following technical scheme:
the giant salamander oligopeptide health-care product comprises the following raw materials in parts by weight: 20-30 parts of giant salamander oligopeptide, 3-5 parts of liquorice extract and 2-4 parts of kudzu root extract.
Continuous experiments show that when the liquorice extract and the kudzu root extract are compounded with the giant salamander oligopeptide, the bitter taste and the fishy smell of the giant salamander oligopeptide are remarkably reduced; therefore, the invention considers that the 3 raw materials can be compounded to prepare the health care product, on one hand, the nutritive value and the efficacy of the giant salamander oligopeptide are kept, and simultaneously, the bitter taste and the fishy smell of the giant salamander oligopeptide are reduced, so that the market acceptance of the giant salamander oligopeptide is greatly improved, and the market of the giant salamander deeply-processed product is expanded. The health product can be prepared into powder, oral liquid or other allowable food additives to form a final product.
In addition, the licorice extract has the effects of invigorating spleen and replenishing qi, eliminating phlegm and relieving cough; the kudzu root extract also has the effects of promoting cardiovascular and cerebrovascular blood flow, improving memory, enhancing immunity and the like. The extracts are all alcohol extracts.
In consideration of taste requirements and nutritional requirements, other substances can be optionally added as raw materials when preparing the health care product, and the health care product can specifically comprise the following components in parts by weight: 2-4 parts of schisandra chinensis polysaccharide, 5-7 parts of blackberry juice and 4-6 parts of apple juice.
Wherein the Schisandra chinensis polysaccharide has the effect of improving intestinal tract; the blackberry has the effects of resisting aging, resisting oxidation, reducing blood pressure, reducing blood fat and the like, and meanwhile, the anthocyanin contained in the blackberry is used as a natural water-soluble pigment, so that the attractive effect of the product can be improved. The apple juice is sour, sweet and delicious, is rich in nutrient components such as sugar, protein, various trace elements, various vitamins and the like, can improve the body function, and reduces the probability of heart disease and arteriosclerosis.
In some preferred embodiments, the giant salamander oligopeptide is obtained by a specific enzymolysis process explored by taking a giant salamander whole fish as a raw material, and the content of the peptide with the molecular weight of less than 600Da is more than 80%, and the content of the peptide with the molecular weight of less than 2000Da is more than 99% (in addition, the obtained giant salamander oligopeptide also has a certain kidney tonifying effect). The giant salamander oligopeptide is specifically obtained by carrying out enzymolysis on giant salamander whole fish by using a compound enzyme; the complex enzyme comprises papain, trypsin and flavourzyme, and the preparation process comprises the following steps:
(1) removing gills and intestinal tracts of giant salamanders, cleaning to obtain meat paste, and adding water to the meat paste to prepare meat pulp by a colloid mill;
(2) adding papain in an amount of 0.1-0.3% of the pulp, trypsin in an amount of 0.1-0.3% of the pulp and flavourzyme in an amount of 0.05-0.1% of the pulp, performing enzymolysis at 45-55 deg.C for 4-8h, and inactivating enzyme; filtering, centrifuging and taking supernatant to obtain the giant salamander oligopeptide.
Preferably, the enzyme deactivation conditions are: 15min at 95 ℃; the centrifugation conditions were 5000-.
The invention has the beneficial effects that: according to the invention, the giant salamander oligopeptide, the licorice extract and the kudzu root extract are compounded as raw materials, so that the bitter taste and the fishy smell of the giant salamander oligopeptide are greatly reduced, the market acceptance is greatly improved, and the market of the giant salamander deep-processed product is expanded.
Drawings
FIG. 1 is a graph showing the results of molecular weight distribution detection of giant salamander whole fish oligopeptide.
Detailed Description
The concept and technical effects of the present invention will be clearly and completely described below in conjunction with the embodiments and the accompanying drawings to fully understand the objects, aspects and effects of the present invention.
Example 1:
a preparation method of giant salamander whole fish oligopeptide comprises the following steps:
(1) pulping: removing gills and intestinal tracts of giant salamanders, cleaning, stirring into meat paste by a meat stirrer, adding a small amount of water, and grinding by a colloid mill for three times to prepare meat pulp;
(2) enzymolysis: adding papain 0.3%, trypsin 0.1% and flavourzyme 0.06% in a ratio of 1:4 into the meat pulp, slowly stirring and performing enzymolysis for 4h in a heat-preserving container at 50 ℃, and inactivating the enzyme for 15min at 95 ℃;
(3) centrifuging: filtering, centrifuging the filtrate in a centrifuge at 10000rpm, and taking the supernatant to obtain the giant salamander whole fish oligopeptide.
The molecular weight distribution of the peptide of the giant salamander oligopeptide is detected according to the standard method of GB/T22729-2008, and the result is shown in FIG. 1. As can be seen from FIG. 1, the content of the peptides with the molecular weight of less than 600Da in the giant salamander whole fish oligopeptide is more than 80%, and the content of the peptides with the molecular weight of less than 2000Da is more than 99%.
Meanwhile, 218.91uM is selected as the molding concentration to establish a hydrogen peroxide induced HepG2 oxidative damage model through experiments. By means of cytotoxicity experiment results, results show that the giant salamander whole fish oligopeptide has a protective effect on oxidative damage HepG2 cells within the range of 2-100 mu g/mL, and the giant salamander whole fish oligopeptide with the concentration of 10 and 100 mu g/mL is selected for next-step exploration of an antioxidant mechanism. The detection by a flow cytometer shows that: the giant salamander whole fish oligopeptide can relieve the blockage of HepG2 cells in the G1 stage, has the most obvious effect of relieving the blockage of the G1 stage in high concentration, and can inhibit H202Induces apoptosis of oxidative damage HepG2 cells. The giant salamander whole fish oligopeptide with different concentrations can reduce H202Inducing and damaging the active oxygen level in HepG2 cells, having a powerful scavenging effect on the active oxygen in HepG2 cells, and presenting an obvious dose dependence relationship. Intervention H on the andrias davidianus whole fish oligopeptide2O2The biochemical indexes in the system for inducing the oxidative damage of the cells are detected, and the experimental result shows that: in HepG2 cells, the giant salamander whole fish oligopeptide can improve SOD activity, CAT and GSH-PX enzyme activity, GSH and MDA content, effectively eliminate peroxy free radicals in cells, prevent lipid peroxidation and reduce oxidation level in cells, thereby playing a role in protecting cells from oxidative damage. Furthermore, the WB results show that the giant salamander whole fish oligopeptide can pass throughExcessive free radicals in cells in vivo are removed, so that the expression of apoptosis-related proteins such as Caspase-3, Bax and the like is reduced, the expression of anti-apoptosis protein Bcl-2 is increased, the apoptosis of HepG2 cells is inhibited, and the protective effect of resisting oxidative damage is achieved.
Example 2:
the giant salamander oligopeptide health-care product comprises the following raw materials in parts by weight: 26 parts of giant salamander oligopeptide, 4 parts of liquorice extract, 3 parts of kudzu root extract, 3 parts of schisandra chinensis polysaccharide, 6 parts of blackberry juice and 5 parts of apple juice, which are prepared in example 1. The raw materials are mixed with 20 parts of water to prepare the giant salamander oligopeptide oral liquid.
During the actual production process, the schisandra polysaccharide, the blackberry juice and the apple juice can be selectively added; allowable food additives or other auxiliary components can be added according to the requirement; it can also be made into oral liquid, powder, etc.
Example 3:
the following oral liquid was subjected to sensory evaluation for fishy smell by scoring method, and 10 persons sensitive to smell and taste were invited to form an evaluation group, and the fishy smell of the oral liquid was scored by smelling first and then tasting. Deionized water is used as a contrast, the score is 0, the larger the score is, the heavier the fishy smell is, the highest score is 5, and the fishy smell degrees are sequentially heavy, heavier, common, lighter, slightly less and absent, which respectively correspond to 5, 4, 3, 2, 1 and 0.
A first group: the giant salamander oligopeptide health-care product comprises the following raw materials in parts by weight: 26 parts of giant salamander oligopeptide, 4 parts of liquorice extract and 3 parts of kudzu root extract prepared in the embodiment 1; the raw materials are mixed with 10 parts of deionized water uniformly to form the oral liquid.
Second group: the giant salamander oligopeptide health-care product comprises the following raw materials in parts by weight: 26 parts of giant salamander oligopeptide, 4 parts of red date extract and 3 parts of mulberry leaf extract prepared in example 1; the raw materials are mixed with 10 parts of deionized water uniformly to form the oral liquid.
Third group: blank group, namely, 26 parts of giant salamander oligopeptide prepared in example 1 and 10 parts of deionized water are mixed uniformly to form oral liquid.
TABLE 1
The results are shown in table 1, and it can be seen from table 1 that the bitter taste and the fishy smell of the oral liquid of the first group are almost not existed, which also proves that the bitter taste and the fishy smell of the giant salamander oligopeptide can be really and remarkably reduced by compounding the licorice extract, the kudzu root extract and the giant salamander oligopeptide.
The above description is only a preferred embodiment of the present invention, and the present invention is not limited to the above embodiment, and the present invention shall fall within the protection scope of the present invention as long as the technical effects of the present invention are achieved by the same means. The invention is capable of other modifications and variations in its technical solution and/or its implementation, within the scope of protection of the invention.
Claims (7)
1. The giant salamander oligopeptide health-care product is characterized by comprising the following raw materials in parts by weight: 20-30 parts of giant salamander oligopeptide, 3-5 parts of liquorice extract and 2-4 parts of kudzu root extract.
2. The giant salamander oligopeptide health product according to claim 1, which is characterized by further comprising the following raw materials in parts by weight: 2-4 parts of schisandra chinensis polysaccharide, 5-7 parts of blackberry juice and 4-6 parts of apple juice.
3. The giant salamander oligopeptide health product according to claim 1, wherein the content of peptides with molecular weight of less than 600Da in the giant salamander oligopeptide is more than 80%, and the content of peptides with molecular weight of less than 2000Da is more than 99%.
4. The giant salamander oligopeptide health product according to claim 3, wherein the giant salamander oligopeptide is obtained by enzymatic hydrolysis of giant salamander whole fish with a complex enzyme; the complex enzyme comprises papain, trypsin and flavourzyme.
5. The giant salamander oligopeptide health product according to claim 4, wherein the giant salamander oligopeptide is prepared by the following process:
(1) removing gills and intestinal tracts of giant salamanders, cleaning to obtain meat paste, and adding water to the meat paste to prepare meat pulp by a colloid mill;
(2) adding papain in an amount of 0.1-0.3% of the pulp, trypsin in an amount of 0.1-0.3% of the pulp and flavourzyme in an amount of 0.05-0.1% of the pulp, performing enzymolysis at 45-55 deg.C for 4-8h, and inactivating enzyme; filtering, centrifuging and taking supernatant to obtain the giant salamander oligopeptide.
6. The giant salamander oligopeptide health product of claim 5, wherein the enzyme deactivation conditions are as follows: and (3) at 95 ℃ for 15 min.
7. The giant salamander oligopeptide health product according to claim 5, wherein the centrifugation conditions are 5000-14000 rpm.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108294225A (en) * | 2018-01-29 | 2018-07-20 | 荣成鸿德海洋生物科技有限公司 | A kind of de- bitter fishy-removing-method of enzymolysis of fish proteins polypeptide liquid |
| CN109007642A (en) * | 2018-07-20 | 2018-12-18 | 雷玲 | A kind of catfish powder |
| CN109221980A (en) * | 2018-09-18 | 2019-01-18 | 江西鼎中科技有限公司 | A kind of biologic fishy smell removing technology of crucian roe |
| CN113637717A (en) * | 2021-06-24 | 2021-11-12 | 山东裕盛生物科技有限公司 | Andrias davidianus oligopeptide and preparation method and application thereof |
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2022
- 2022-02-18 CN CN202210151257.1A patent/CN114391656A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108294225A (en) * | 2018-01-29 | 2018-07-20 | 荣成鸿德海洋生物科技有限公司 | A kind of de- bitter fishy-removing-method of enzymolysis of fish proteins polypeptide liquid |
| CN109007642A (en) * | 2018-07-20 | 2018-12-18 | 雷玲 | A kind of catfish powder |
| CN109221980A (en) * | 2018-09-18 | 2019-01-18 | 江西鼎中科技有限公司 | A kind of biologic fishy smell removing technology of crucian roe |
| CN113637717A (en) * | 2021-06-24 | 2021-11-12 | 山东裕盛生物科技有限公司 | Andrias davidianus oligopeptide and preparation method and application thereof |
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