CN114376195B - Whole flax seedling powder rich in secoisolaricol and its production method - Google Patents

Abstract

The invention relates to a full powder of flax seedlings rich in secoisolariciresinol and a production method thereof, belonging to the technical field of food processing, flax seeds are planted in hydrogen-rich water, and mixed aqueous solution of NaCl and gamma-polyglutamic acid is used for spraying and sprouting to enrich the flax seedlings with SECO; the flax seedling is taken as a raw material, and is prepared by vacuum freeze-drying, supersonic jet milling, sterilization and packaging. The product is suitable for industrial production, the SECO content in the product can reach 150-400 mg/100 g, and the product is a health food with edible safety.

Description

Whole flax seedling powder rich in secoisolaricol and its production method

technical field

The invention relates to a whole flax seedling powder rich in secoisolaricineol SECO and a production method thereof, belonging to the technical field of food processing.

Background technique

Germination is an important means to improve the nutritional value and functional properties of plant seeds harmlessly and at low cost. Many physiological metabolic changes occur after the grain absorbs water and germinates, accompanied by complex biochemical metabolism, which promotes the improvement of the nutritional content of the grain, reduces or eliminates the original harmful substances or anti-nutritional substances, and enriches the functional active ingredients. After the flaxseed is germinated, the content of cyanide decreases, the content of polyphenols such as amino acids, vitamin C, minerals, lignans, and flavonoids increases, and the physiological functions are strengthened. Among them, the main form of lignans in flax seeds is Secoisolaricisol diglucoside (SDG), which needs to be converted into the intermediate product Secoisolaricisol (SECO) by intestinal bacteria before it can be absorbed and utilized by the human body. During the germination process, part of SDG will be directly converted into its aglycone form—SECO. Studies have shown that SECO has stronger physiological effects than SDG, such as antioxidant, breast cancer prevention, diabetes suppression, and menopausal symptoms in women. Therefore, it is easier to be absorbed and utilized by the human body when added to food as a functional food , the health care effect is more prominent, therefore, simple and efficient acquisition of SECO-rich products is of great significance for giving full play to the application value of flax.

At present, research on flax lignans at home and abroad is mostly focused on the extraction and purification process. Generally, the flaxseed cake after oil extraction or the shell-rich part after shelling is used as raw material to obtain lignans through enzymatic hydrolysis or acid-base hydrolysis. The extraction process is complicated, resulting in high cost of lignans. However, using the characteristics of plant growth and development to enrich lignans is simple and easy, greatly reduces production costs, and is safe, reliable and convenient to eat. At present, flax sprouts have been included in the scope of green food sprout vegetables by the Ministry of Agriculture, but the relevant research is relatively lagging behind. The patent (publication number CN 110199613 A, public date September 6, 2019) discloses "a method for cultivating high-calcium nutritious flax sprouts and its special seed soaking method", the patent (publication number CN 110199614 A, public date September 6, 2019) discloses "a method for cultivating selenium-enriched nutritious flax sprouts", and the patent (publication number CN110352657 A, public date 2019 1 On October 22) disclosed "a cultivation method of zinc-enriched flax sprouts", which belongs to the field of agriculture and only targets specific minerals, without mentioning SECO; the patent (publication number CN 110463523 A, publication date: November 19, 2019) disclosed "a cultivation method for increasing the accumulation of lignans in flax sprouts", which belongs to the field of cultivation of flax sprouts. No. CN104544350 A, published on April 29, 2015) discloses "the production method of linseed sprout powder", which relates to a flax processing technical field, and obtains linseed sprouts after relieving dormancy, and obtains linseed sprout powder after deactivating enzymes and drying, and enriches SECO by using the characteristics of the growth and development of the plant itself without using certain means.

Contents of the invention

In view of the problems existing in the prior art, the present invention aims to provide a flax seedling powder rich in SECO and its production method. In the present invention, the flax seeds are soaked in hydrogen-rich water, sprayed with a mixed aqueous solution of NaCl and γ-polyglutamic acid to germinate, and activate relevant endogenous enzymes, thereby enriching SECO and improving the health care function of the flax seedling powder. The product is rich in amino acids, vitamins, minerals, SECO and other beneficial ingredients, and the whole flax seedlings are crushed and rich in dietary fiber, which greatly improves the health care function of flax seedlings whole powder.

In order to solve the above-mentioned technical problems, the present invention adopts the following technical scheme: flax seeds are soaked in hydrogen-rich water at a certain temperature, sprayed and germinated with a mixed aqueous solution of NaCl and γ-polyglutamic acid, so that the flax seedlings are enriched in SECO, vacuum freeze-dried, crushed by supersonic airflow, sterilized and packaged, and prepared, which specifically includes the following steps:

(1) Spray germination

A. Selection of raw materials: choose brown flax or golden flax with full grains, no pests and diseases, and a germination rate of more than 95%;

B. Disinfection and soaking: After rinsing with deionized water, soak and sterilize in 0.5%~0.8% (v/v) NaClO aqueous solution for 10~15 minutes, and then rinse with deionized water until the pH is neutral; use a pure water hydrogen generator to inject _H2 into the distilled water to make the concentration of _H2 in the hydrogen-rich water reach 0.1-0.4 mmol/L. Soak flax seeds in hydrogen-rich water for 4~5 hours at a temperature of 15~30℃ to make the flax fully absorb water ;

C. Spray germination: Wash the fully absorbent flax seeds and place them in a germination machine, spray them with water for 1-3 days, the conditions are: germination temperature 20-35°C, dark; then spray with a mixed aqueous solution of 40-120 mmol/L NaCl and 0.5-3 g/L γ-polyglutamic acid, and grow under light for 3-7 days; the conditions are: germination temperature 15-25°C, 12 hours of light per day, 12 hours of darkness, light / dark alternately;

(2) Seedling powder preparation: select flax seedlings with a seedling length of 6-10 cm, and use a water shaker to remove surface water after cleaning; quickly freeze the flax seedlings at -120--196 °C for 1.0-3.0 h, then place them in a tray of a freeze dryer, set the temperature at -50-90 °C, freeze for 10-15 min, and then vacuumize at a vacuum degree of 1.0-6.0 Pa. Freeze-dry for 18-30 h; adjust air pressure and clean air temperature, using the supersonic jet crushing technology to obtain 400-1000 mesh flax seedling powder;

(3) Sterilization and packaging: Microwave sterilization is used with an output power of 50-100 KW for 5-10 minutes; then packaged in vacuum aluminum foil, inspected by an X-ray machine, and packaged if there is no abnormality.

The present invention has the following beneficial effects:

(1) In the present invention, flax seeds are soaked in hydrogen-rich water, sprayed with a mixed aqueous solution of NaCl and γ-polyglutamic acid to germinate, and activate related endogenous enzymes to enrich SECO and improve the health care function of flax seedling powder. The product is rich in amino acids, vitamins, minerals, SECO and other beneficial ingredients, and the whole flax seedlings are crushed and rich in dietary fiber, which greatly improves the health care function of flax seedlings whole powder.

(2) The present invention adopts a unique biotransformation technology, and the prepared flax seedling powder is rich in health-care ingredients such as SECO, which is helpful for human body to resist oxidation and improve immunity; the content of soluble dietary fiber is high, which is beneficial to regulate intestinal function.

(3) The present invention adopts vacuum freeze-drying technology to keep active ingredients such as vitamins and chlorophyll in raw materials to the greatest extent, and at the same time uses supersonic airflow pulverization technology to complete pulverization, grading and collection at one time. The product is fine in powder quality and the eating quality is significantly improved.

(4) The production of the product of the present invention is not restricted by seasons, climates and regions, and the utilization rate of agricultural resources is high; and the production technology is advanced, scientific, and environmentally friendly.

Description of drawings

Fig. 1 is _H The influence of concentration on the SECO content of flax sprout whole powder;

Fig. 2 is the influence of NaCl concentration on the SECO content of flax sprout whole powder;

Figure 3 shows the effect of the concentration of γ-polyglutamic acid on the SECO content of the whole powder of flax sprouts.

Detailed ways

The present invention will be further described below in conjunction with specific embodiments. It should be understood that the following examples are only used to illustrate the present invention rather than limit the scope of the present invention, and those skilled in the art can make some non-essential improvements and adjustments based on the content of the above invention.

Example 1

(1) Select flax seeds with full grains and no pests and diseases, soak them in 0.5% (v/v) NaClO aqueous solution for 15 minutes, wash them, soak them in distilled water with _H2 concentration of 0.3 mmol/L at 20°C for 4 hours, then transfer them to a germination machine, spray them with water at 20°C for 3 days in the dark, and then germinate them with 60 mmol/L NaCl and 1.0 g/L γ-polyglutamic acid at 22°C The mixed aqueous solution was sprayed and germinated for 5 days, and the conditions were light 12 h/dark 12 h alternately;

(2) Select the flax seedlings with a seedling length of 9 cm, remove the surface water with a water shaker after cleaning, and then quickly freeze them at -120~-196°C for 1.0~3.0 h, then place them in a tray of a freeze dryer, set the temperature at -50~-90°C, freeze for 10~15 min, and then vacuumize them at a vacuum degree of 1.0~6.0 Pa. Freeze-dry for 18~30 h; 0 mesh flax seedling powder;

(3) Use microwave to sterilize with an output power of 50-100 KW for 5-10 minutes; then pack it in vacuum aluminum foil, check it with an X-ray machine, and pack it if there is no abnormality.

The content of SECO in the product prepared in this example can reach 380 mg/100 g.

Example 2

(1) Select flax seeds with full grains and no pests and diseases, soak them in 0.6% (v/v) NaClO aqueous solution for 13 minutes, wash them and soak them in distilled water with _H2 concentration of 0.2 mmol/L at 25°C for 4 hours, then transfer them to a germination machine, spray them with water at 25°C for 2 days, and then germinate them with 80 mmol/L NaCl and 1.5 g/L γ-polyglutamic acid at 18°C The mixed aqueous solution was sprayed and germinated for 6 days, and the conditions were light 12 h/dark 12 h alternately;

(2) Select flax seedlings with a seedling length of 8 cm, and the preparation method of flax seedling powder is the same as step (2) of embodiment 1;

(3) Sterilization and packaging are the same as step (3) in Example 1.

The content of SECO in the product prepared in this embodiment can reach 320 mg/100 g.

Example 3

(1) Select flax seeds with plump grains and no pests and diseases, soak them in 0.7% (v/v) NaClO aqueous solution for 11 minutes, wash them and soak them in distilled water with a _H2 concentration of 0.15 mmol/L at 30°C for 5 hours, then transfer them to a germination machine, spray them with clean water at 30°C for 3 days in the dark, and then germinate them with 100 mmol/L NaCl and 2.0 g/L γ-polymer at 20°C. Spray germination with glutamic acid mixed aqueous solution for 4 days, under the conditions of light 12 h/dark 12 h alternately;

(2) Select flax seedlings with a seedling length of 6 cm, and the preparation method of flax seedling powder is the same as step (2) of embodiment 1;

(3) Sterilization and packaging are the same as step (3) in Example 1.

The SECO content of the product prepared in this example can reach 250 mg/100 g.

The results are shown in Figure 1, which shows the effect of _H2 concentration on the SECO content of flax sprout powder. The culture conditions were that the selected flaxseeds were soaked and sterilized in 0.5% (v/v) NaClO aqueous solution for 15 minutes, washed and soaked in distilled water with a series of _H2 concentrations (see Figure 1) at 25°C for 5 hours, then transferred to a germination machine, and sprayed with water at 25°C for 8 days. The conditions were light 12 h/dark 12 h alternately; Flax seedlings, the preparation method of flax seedlings whole powder are the same as in Example 1;

Figure 2 shows the effect of NaCl concentration on the SECO content of flax sprout powder. The culture conditions were that the selected flaxseeds were soaked in 0.5% (v/v) NaClO aqueous solution for 15 min, washed and placed in 25°C under H₂Soak in distilled water with a concentration of 0.3mmol/L for 5 hours, then transfer to a germination machine, spray and germinate with water at 25°C for 2 days in the dark, and then spray and germinate with different concentrations of NaCl aqueous solution at 25°C for 6 days, under the condition of alternating light for 12 h and dark for 12 h; choose flax seedlings with a seedling length of 8 cm, and the preparation method of flax seedling powder is the same as in Example 1; the sterilization and packaging methods are the same as in Example 1.

Figure 3 shows the effect of the concentration of γ-polyglutamic acid on the SECO content of flax sprout powder. The culture conditions were that the selected flaxseeds were soaked in 0.5% (v/v) NaClO aqueous solution for 15 minutes, washed and placed in 25°C under H₂Soak in distilled water with a concentration of 0.3 mmol/L for 5 h, then transfer to a germination machine, spray and germinate with water at 25°C for 2 days in the dark, and then spray and germinate with 60 mmol/L NaCl aqueous solution and different concentrations of γ-polyglutamic acid for 6 days at 25°C, under the conditions of 12 h of light and 12 h of darkness; choose flax seedlings with a seedling length of 8 cm, and the preparation method of flax seedling powder is the same as in Example 1; the sterilization and packaging methods are the same as in Example 1.

The embodiments of the present invention have been described in detail above, but these are just examples for easy understanding, and should not be regarded as limiting the scope of the present invention. Similarly, any person skilled in the art can make various possible equivalent changes or substitutions according to the technical solution of the present invention and the description of its preferred embodiments, but all these changes or substitutions should belong to the protection scope of the claims of the present invention.

Claims (1)

1. A production method of full-powder of flax seedlings rich in secoisolariciresinol comprises the following steps:

(1) Selecting plump and pest-free flaxseed, soaking in 0.5% NaClO water solution for 15 min, cleaning, and standing at 20deg.C for H ₂ Soaking 4. 4 h in distilled water with the concentration of 0.3 mmol/L, transferring into a germination machine, spraying clean water in the dark at 20 ℃ for germination for 3d, and spraying mixed aqueous solution of 60 mmol/L NaCl and 1.0 g/L gamma-polyglutamic acid at 22 ℃ for germination for 5 d, wherein the conditions are that illumination 12 h/darkness 12 h are alternated;

(2) Selecting flax seedlings with the seedling length of 9 cm, cleaning, removing surface clear water by a hydro-extracting machine, quick-freezing at-120 to-196 ℃ for 1.0-3.0 h, placing in a tray of a freeze dryer, setting the temperature to-50 to-90 ℃, freezing for 10-15 min, vacuumizing to 1.0-6.0 Pa, and freeze-drying for 18-30 h; taking out, and obtaining 400-1000 mesh flax seedling whole powder by using a supersonic jet milling technology;

(3) Microwave sterilization is adopted, the output power is 50-100 KW, and the time is 5-10 min; packaging with vacuum aluminum foil, checking with X-ray machine, and packaging after no abnormality; the content of the secoisolariciresinol in the product reaches 380 mg/100 g.