CN114342960B

CN114342960B - Solid slow-release compound avermectin preparation for killing pine wood nematode and production process thereof

Info

Publication number: CN114342960B
Application number: CN202210107763.0A
Authority: CN
Inventors: 胡杨
Original assignee: Zhejiang Academy of Forestry
Current assignee: Zhejiang Academy of Forestry
Priority date: 2022-01-28
Filing date: 2022-01-28
Publication date: 2023-03-24
Anticipated expiration: 2042-01-28
Also published as: CN114342960A

Abstract

The invention relates to the technical field of pesticides, and discloses a solid slow-release compound avermectin preparation for killing pine wood nematodes, which comprises avermectin, polysiloxane, chinese torreya essential oil and semisynthetic fatty glyceride; the preparation adopts a solid sustained-release preparation, can prolong the sustained-release time, effectively prolongs the effective period of the control of the injection rod agent, and uses degradable materials, thereby being green and environment-friendly.

Description

Solid slow-release compound avermectin preparation for killing pine wood nematode and production process thereof

Technical Field

The invention relates to the technical field of pesticides, and particularly relates to a solid slow-release compound avermectin preparation for killing pine wood nematodes and a production process thereof.

Background

Pine wilt disease is also called pine wilt disease, and is a destructive disease. It is transmitted in the pine body by the vector insects such as Monochamus alternatus, etc., thereby causing the pine disease. Pine infected by pine wood nematode has yellow brown or red brown needle leaves, wilting and drooping, resin secretion is stopped, the trunk can observe the invasion hole or egg laying trace of longicorn, and the whole diseased tree is withered and died. Nematodes can kill pine trees within 6 months and are the main pests that destroy large pieces of pine forest.

Pine wilt disease occurs in the united states, canada, mexico, japan, korea, etc., and attacks hong kong in china in the 20 th century in the 80 th era, almost destroying the pinus massoniana forest widely distributed in hong kong. In 1982, the mountain is found for the first time in Nanjing, and then several disease centers are formed in Anhui, shandong, zhejiang, guangdong and the like in sequence and spread to the periphery, so that local areas of the provinces are subjected to parallel flow and disaster formation, and a large number of pine trees are died.

At present, 6 enterprises in Zhejiang province have registered the injection of the immunizing agent pesticide into the trunk of pine. 150 ten thousand old pine trees are protected by punching holes and injecting medicine every year. As early as 2005, pine immunoprophylaxis tests were developed in local areas in Ningbo city, and were popularized and applied in large areas in 2009 with a usage amount of 60-90 million bottles per year. In 2005, up to now, 1091 ten thousand bottles of bursaphelenchus xylophilus disease immune preparation were injected cumulatively, the capital investment was nearly 6700 ten thousand yuan, 810 thousand plants of key pines and ancient pines were injected cumulatively, and the area of the pines was protected by 40 ten thousand mu. The use of the trunk immune preparation realizes the individualized accurate prevention of the pine, lays a foundation for reducing the disease base number of the pine wood nematode disease and extinguishing an epidemic area, and plays a great role in protecting ancient pines, landscape pine forests and ecological pine forests from being infected by the disease. At present, ancient pine communities such as voyao brook, , the temple of ajowan, the peace hippocampus, the yao quiet country, etc., are well preserved, and natural landscapes such as voyao brook, the cixi volt dragon hill, the ninghai hop mountain, etc., which mainly comprise pine forest vegetation, are well preserved. In the process of technical popularization, the immune preparation has excellent prevention effect on the pine wood nematode disease, has good prevention effect on coleoptera and lepidoptera pests which harm trees, and provides a new technical means and thought for preventing and controlling forest diseases and insect pests. The injection of the abamectin or emamectin benzoate into the pine trees has the advantages of small dosage, environmental protection, safety, simple and convenient operation and the like, does not produce secondary pollution and phytotoxicity to the environment and natural enemies, is a green control technology, and has the control effect of one-time injection and three-year effectiveness.

At present, most of the abamectin or emamectin benzoate injection preparations in the market are liquid preparations with the concentration of 2-10%, the injection time is influenced by pine resin secretion, the application time is limited in specific months in winter every year, the injection time is short, and the control effective period is generally below 3 years. For example, the nematicidal composition containing abamectin and furfural has the validity period of 2-3 months for annual crops and 4-6 months for perennial crops, wherein the authorization notice number is CN 103430950B. Therefore, how to improve the effective period of the pine wood nematode killing preparation is the most important direction of the current research, the application provides the solid slow-release compound avermectin pine wood nematode killing preparation, and the solid slow-release preparation is adopted, so that the release time of the preparation is effectively prolonged, and the control effect is improved.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide the solid slow-release compound abamectin preparation for killing the pine wood nematodes, which adopts the solid slow-release preparation, can prolong the slow-release time, effectively prolongs the effective period of the control of the injection rod agent, uses degradable materials and is green and environment-friendly.

In order to achieve the above purpose, the invention provides the following technical scheme:

a solid sustained-release compound avermectin preparation for killing pine wood nematode comprises avermectin, polysiloxane, torreya grandis essential oil and semisynthetic fatty acid glyceride.

Preferably, the preparation comprises 5-25% of abamectin, 8-20% of polysiloxane, 12-18% of Chinese torreya essential oil and 37-75% of semisynthetic fatty glyceride according to percentage.

Preferably, the preparation comprises 20 percent of abamectin, 18 percent of polysiloxane, 15 percent of Chinese torreya essential oil and 47 percent of semisynthetic fatty glyceride.

The preparation of the invention adopts semisynthetic fatty glyceride as a dispersant, improves the concentration of active ingredients, reduces the volume of the preparation, and the concentration of the existing common liquid injection rod agent is generally 1.2-2%, the dosage is 50ml, and the total drug concentration is 1.2-2 g/branch. When the concentration of the abamectin serving as an active ingredient in the preparation is 20 percent, the total drug concentration is 2.125 g/branch, and the preparation has high drug concentration and small volume which is 2.3 times smaller than that of a rod injection bottle.

Because healthy pine trees are filled with rosin, although the effective components and the formulations of the commercially available pesticides are the same, the auxiliary agents are very different, the pesticide after being injected to the trunk is blocked by the rosin, the infusion conductivity in the pine trees is very poor, and the pesticide cannot be used for trunk injection; the preparation of the invention adopts polysiloxane in cooperation, has strong permeability, small surface tension and strong solubility to rosin, and ensures that the effective components can overcome the rosin obstacle and can be rapidly dispersed and conducted in pine bodies;

meanwhile, the preparation adopts polysiloxane as a solid sustained release agent, the sustained release agent is a temperature-variable compound, the dissolution of the polysiloxane is accelerated along with the gradual increase of the environmental temperature, the temperature change range is 25-34 ℃, when the preparation is used for the first time, the dissolution speed of the polysiloxane is very slow because of the lower environmental temperature, the dissolution speed of the polysiloxane is gradually accelerated along with the gradual increase of the temperature, and the dissolution speed of the polysiloxane is gradually increased along with the continuous enhancement of the generation and the flow of turpentine in pine, and the temperature is changed in a round and round season, so that the drug release speed of the preparation is from slow to fast and from fast to slow, and the whole dissolution speed is up to 2-3 years which is ten times that of a sucker rod injection agent; the torreya essential oil is used as an auxiliary agent, and alpha-pinene, beta-pinene and other specific attractants in the torreya essential oil are used for effectively attracting nematodes to gather around the medicament to generate a larger killing effect; and can kill infested nematodes and longicorn eating stalks, and has very obvious control effect.

Preferably, the polysiloxane includes, but is not limited to, polydimethylsiloxane, trimethylmethoxysilane, allyloxytrimethylsilane.

Preferably, the semisynthetic fatty glyceride is 34-type semisynthetic fatty glyceride.

A production process of a solid slow-release compound avermectin preparation for killing pine wood nematodes comprises the following steps: weighing abamectin, polysiloxane and Chinese torreya essential oil in the raw material medicines, adding the raw material medicines into a heating stirring tank, and stirring at a constant speed until the abamectin is completely dissolved; slowly adding melted semisynthetic fatty glyceride, stirring at uniform speed until semisynthetic fatty glyceride and other medicines are completely mixed, stopping stirring, heating while maintaining temperature, slowly injecting the hot medicinal liquid into a mold, cooling, demoulding, and packaging.

Preferably, the temperature is maintained at 80 ℃ after the stirring is stopped.

Preferably, the stirring speed is 120 rpm.

Compared with the prior art, the invention has the beneficial effects that:

1) The sustained-release time is prolonged by 2-3 years, which is ten times of that of the injection rod agent, so that the effective period of the injection rod agent is effectively prolonged;

2) The operation is simple, the medicine is accurately applied, the dosage of chemical pesticides is reduced compared with the dosage of injection rods, spraying and soil application, the cost is saved, and the cost of medicine bottles is reduced;

3) The used materials are safe to soil, water resources, pesticide application people and beneficial insects and natural enemies of non-target trees; the material can be degraded, so that the environment is protected;

4) Chinese torreya essential oil is added as a local nematode trapping agent, so that the killing effect is improved;

the using proper period of the product is 12 months to 3 months (pine dormancy) each year, or the use proper adjustment is made according to the local climate condition, compared with the prior liquid preparation such as abamectin or emamectin benzoate, the application time of the product is limited to a specific month in winter each year, and the application period of the product is more loose.

Detailed Description

Example 1

The solid slow-release compound avermectin preparation for killing pine wood nematodes comprises 5 percent of avermectin, 8 percent of polysiloxane, 12 percent of Chinese torreya essential oil and 75 percent of semisynthetic fatty glyceride.

Example 2

The solid slow-release compound avermectin preparation for killing pine wood nematodes comprises 15 percent of avermectin, 12 percent of polysiloxane, 13 percent of Chinese torreya essential oil and 60 percent of semisynthetic fatty glyceride.

Example 3

The solid slow-release compound avermectin preparation for killing pine wood nematodes comprises, by percentage, 20% of avermectin, 18% of polysiloxane, 15% of Chinese torreya essential oil and 47% of semisynthetic fatty glyceride.

Example 4

The solid slow-release compound avermectin preparation for killing pine wood nematodes comprises 25% of avermectin, 20% of polysiloxane, 18% of Chinese torreya essential oil and 37% of semisynthetic fatty glyceride.

1. The production process of the solid slow-release compound avermectin preparation for killing pine wood nematodes comprises the following steps:

sequentially weighing abamectin, polysiloxane and torreya grandis essential oil in the raw material medicines according to the proportion, adding the abamectin, polysiloxane and torreya grandis essential oil into a heating and stirring tank, and uniformly stirring at a constant speed of 120 revolutions per minute until the abamectin is completely dissolved; slowly adding melted semisynthetic fatty glyceride, stirring at 120 rpm until semisynthetic fatty glyceride and other medicines are completely mixed, stopping stirring, heating, maintaining the temperature at 80 deg.C, slowly injecting the hot medicinal liquid into a mold, cooling, demoulding, and packaging.

2. The use method of the solid slow-release compound avermectin preparation for killing pine wood nematodes comprises the following steps:

1) Punching: the surface of a trunk 50-100cm away from the ground is smooth, no dead knot exists, no damage part is punched, an included angle of 30-40 degrees is formed between a puncher rod and the trunk, the included angle is upward, the diameter of a hole is 1.3-1.5cm, and the depth is 6-8cm;

2) Entering a tree: pressing the bottom of the medicine tube and inserting the medicine tube into the hole to ensure that the medicine gradually permeates into the xylem and is conveyed upwards through the guide tube;

3) The dosage is as follows: the dosage should be sufficient, and enough medicine should be injected according to the diameter at breast height of the tree, and the specific dosage can be shown in table 1.

TABLE 1 comparison table of dosage of different Larix Gmelini preparations

Note that:

1. can not be used for landscape modeling pine trees.

2. Other pesticides, organic solvents, nutrient solutions and the like are not required to be mixed when in use.

3. The pesticide residue period of the pesticide on pine trees is long, and the pesticide is used with cautions in areas where pine pollen and pine nuts are collected.

The release period of the preparation of the embodiments 1-4 is as long as 2-3 years, the pesticide effect on the pine wood nematodes is as high as 75-80%, when the concentration of the active ingredient abamectin in the preparation is 20%, the pine mortality rate caused by the pine wood nematode disease is less than or equal to 1%, and the standard that the pine mortality rate caused by the pine wood nematode disease in the file of the forest bureau in Zhejiang province is less than 1% is met; in addition, the preparations of the embodiments 1 to 4 can kill infested nematodes and longicorn eating stalks, and the control effect is very obvious.

The foregoing is a preferred embodiment of the present invention, and it will be apparent to those skilled in the art that various modifications and improvements can be made without departing from the spirit of the invention, and the scope of the invention shall be limited only by the appended claims.

Claims

1. A solid slow-release compound avermectin preparation for killing pine wood nematodes is characterized by comprising 5% -25% of avermectin, 8% -20% of polysiloxane, 12% -18% of Chinese torreya essential oil and 37% -75% of semisynthetic fatty glyceride.

2. The solid slow-release compound avermectin preparation for killing pine wood nematodes according to claim 1, which is characterized by comprising 20% of avermectin, 18% of polysiloxane, 15% of Chinese torreya essential oil and 47% of semisynthetic fatty glyceride.

3. The solid slow-release compound abamectin pine wood nematode killing preparation according to claim 1, wherein the polysiloxane comprises any one or a combination of more than two of polydimethylsiloxane, trimethylmethoxysilane and allyloxytrimethylsilane.

4. The solid slow-release compound avermectin bursaphelenchus xylophilus killing preparation according to claim 1, wherein the semi-synthetic fatty acid glyceride is 34 type semi-synthetic fatty acid glyceride.

5. The production process of the solid slow-release compound abamectin pine wood nematode killing preparation according to any one of claims 1 to 4, characterized by comprising the following steps: weighing abamectin, polysiloxane and Chinese torreya essential oil in the raw material medicines, adding into a heating stirring tank, and stirring at a constant speed until the abamectin is completely dissolved; slowly adding melted semisynthetic fatty glyceride, stirring at uniform speed until semisynthetic fatty glyceride and other medicines are completely mixed, stopping stirring, heating while maintaining temperature, slowly injecting the hot medicinal liquid into a mold, cooling, demoulding, and packaging.

6. The production process of the solid slow-release compound abamectin pine wood nematode killing preparation according to claim 5, characterized in that the temperature is maintained at 80 ℃ after the stirring is stopped.

7. The production process of the solid slow-release compound abamectin pine wood nematode killing preparation according to claim 5, characterized in that the stirring speed is 120 r/min.