CN114342599B - Method for breaking cranberry seed dormancy by exogenous hormone - Google Patents
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Abstract
The invention relates to a method for breaking the dormancy of cranberry seeds by exogenous hormone, which comprises the following steps: in the seed soaking treatment of the cranberry seeds, any one hormone of melatonin, gibberellin and kinetin is added into water for seed soaking to prepare a hormone solution, and the cranberry seeds are soaked in the hormone solution. The invention can greatly improve the germination rate of the cranberry seeds by applying the hormone to the cranberry seed soaking treatment and providing proper hormone types and application time.
Description
1. The technical field is as follows:
the invention relates to the field of vegetable cultivation, in particular to a method for breaking cranberry seed dormancy by exogenous hormones.
2. Background art:
cranberry (Vaccinium macrocarpon air.) also known as cranberry, cranberry and cranberry is a genus of Vaccinium myrtillus (Oxycocos) of the genus Vaccinium of the family Ericaceae, is an evergreen shrub, mainly grows in cool zones of northern hemisphere, is favored by acidic soil and has high organic carbon content. Dark pink flowers, racemes and berry fruits, wherein the mature fruits are bright red, and the straight stems of the fruits are 2 to 5cm. Cranberry berries contain air and float on the water. The cranberry fruit is rich in nutritional ingredients such as protein, fat, carbohydrate, vitamins, minerals and the like, also contains a large amount of physiologically active substances such as anthocyanin, flavonol, ellagic acid, cycloether terpene, resveratrol and the like, and is a natural antibacterial health-care fruit. The cranberry fruits are rich in VA, VB6, VC, VE, riboflavin, nicotinic acid, folic acid and the like, the content of VC is 6 times of that of common fruits, and the content of anthocyanin is 4-5 times of that of common fruits, so that the cranberry fruits have the effects of removing free radicals, enhancing the elasticity of blood vessels and preventing cardiovascular and cerebrovascular diseases. Resveratrol belongs to polyphenol substances, has antibacterial and anti-inflammatory effects, and ellagic acid has obvious inhibiting effect on multiple cancer cell cancers such as colon cancer, esophageal cancer, liver cancer, lung cancer, etc. Therefore, the cranberry is widely applied in the fields of health food and medical treatment and health.
In China, cranberries are mainly bred in wild species and mainly distributed in major and minor mountaineering areas, large-scale artificial planting is started in the ChangYuan city of Heilongjiang in 2015, and the propagation mode mainly adopts asexual propagation such as root division, cuttage and the like. The cranberry seeds are small, and the thousand seed weight is about 0.85 g; the dormancy period is long, can be up to 6 months at 4 ℃, the germination condition of the seeds is harsh, the sexual propagation of the cranberry is limited, and at present, no report about the propagation of the cranberry seeds is seen in China.
3. The invention content is as follows:
the invention aims to provide a method for breaking the dormancy of cranberry seeds by exogenous hormones, which is used for solving the problems of low extraction rate, high reaction temperature and easiness in oxidation of triterpenoids in the prior art.
The technical scheme adopted by the invention for solving the technical problem is as follows: the method for breaking the dormancy of cranberry seeds by using the exogenous hormone comprises the following steps: when cranberry seeds are soaked, any one hormone of melatonin, gibberellin and kinetin is added into water for soaking the cranberry seeds to prepare a hormone solution, and the cranberry seeds are soaked in the hormone solution.
In the scheme, the hormone is melatonin, the concentration range of the melatonin in the prepared melatonin solution is 50-400mg/kg, the melatonin solution is treated for 10min under the frequency condition of 20000Hz by a water treatment device, and then cranberry seeds are soaked.
In the scheme, the hormone is gibberellin, the concentration range of the gibberellin in the prepared gibberellin solution is 200-1200 mg/kg, the gibberellin solution is treated for 10min under the condition of 20000Hz frequency through a water treatment device, and cranberry seeds are soaked.
In the scheme, hormone is kinetin, the concentration range of the kinetin in the prepared kinetin solution is 5-80mg/kg, and the kinetin solution is treated for 10min under the condition of 20000Hz frequency by a water treatment device and then soaked in cranberry seeds.
The cranberry seeds were rinsed twice with pH =4.0 distilled water prior to seed soaking in the above protocol.
The seed soaking time of the cranberry seeds in the scheme is 22h.
The cranberry seed soaking container in the scheme is a plastic culture dish.
Has the advantages that:
1. according to the method, the hormone is applied during the seed soaking treatment of the cranberry seeds, and the proper hormone type and application time are provided, so that the germination rate of the cranberry seeds can be greatly improved.
2. The method breaks the condition of cranberry seed dormancy, provides a theoretical basis for cranberry seed propagation and large-scale artificial planting, and provides a technical guarantee for cranberry seed rapid germination.
4. The specific implementation mode is as follows:
example 1:
the method for breaking the cranberry seed dormancy by using the exogenous hormone comprises the following steps: in the seed soaking treatment of the cranberry seeds, any one hormone of melatonin, gibberellin and kinetin is added into water for seed soaking to prepare a hormone solution, and the cranberry seeds are soaked in the hormone solution.
Example 2:
the method for breaking the dormancy of cranberry seeds by using the exogenous hormone comprises the following steps: melatonin is added during the seed soaking treatment of the cranberry seeds, and the specific method comprises the following steps: preparing a melatonin solution, wherein the concentration range of the melatonin in the prepared melatonin solution is 50mg/kg, treating the melatonin solution for 10min under the frequency condition of 20000Hz by a water treatment device, and then soaking seeds of the cranberries.
Example 3:
the method for breaking the dormancy of cranberry seeds by using the exogenous hormone comprises the following steps: gibberellin is added during cranberry seed soaking treatment, and the specific method comprises the following steps: preparing gibberellin solution, wherein the concentration range of gibberellin in the prepared gibberellin solution is 1200mg/kg, treating the gibberellin solution for 10min under the condition of 20000Hz frequency through a water treater, and then soaking the cranberry seeds for 22 hours.
Example 4:
the method for breaking the cranberry seed dormancy by using the exogenous hormone comprises the following steps: adding kinetin during cranberry seed soaking treatment, wherein the specific method comprises the following steps: preparing an kinetin solution, wherein the concentration range of kinetin in the prepared kinetin solution is 5mg/kg, and soaking cranberry seeds with the kinetin solution, wherein the cranberry seeds are washed twice with distilled water with the pH =4.0 before being soaked.
Example 5:
the method for breaking the cranberry seed dormancy by using the exogenous hormone comprises the following steps: gibberellin is added during cranberry seed soaking treatment, and the specific method comprises the following steps: preparing a gibberellin solution, wherein the concentration range of gibberellin in the prepared gibberellin solution is 200mg/kg, treating the gibberellin solution for 10min under the frequency condition of 20000Hz through a water treatment device, then using the gibberellin solution for cranberry seed soaking, wherein the seed soaking time is 22 hours, a cranberry seed soaking container is a plastic culture dish, and the cranberry seed soaking container is washed twice with distilled water with the pH =4.0 before being soaked.
Example 6:
the method for breaking the dormancy of cranberry seeds by using the exogenous hormone comprises the following steps: adding kinetin during cranberry seed soaking treatment, wherein the specific method comprises the following steps: preparing an kinetin solution, wherein the concentration range of kinetin in the prepared kinetin solution is 80mg/kg, and soaking cranberry seeds with the kinetin solution, wherein the cranberry seeds are washed twice with distilled water with the pH =4.0 before being soaked.
Example 7:
the method for breaking the dormancy of cranberry seeds by using the exogenous hormone comprises the following steps: adding kinetin during cranberry seed soaking treatment, wherein the specific method comprises the following steps: preparing an kinetin solution, wherein the concentration range of kinetin in the prepared kinetin solution is 40mg/kg, and soaking cranberry seeds with the kinetin solution, wherein the cranberry seeds are washed twice with distilled water with the pH =4.0 before being soaked.
Example 8:
the method for breaking the dormancy of cranberry seeds by using the exogenous hormone comprises the following steps: gibberellin is added during cranberry seed soaking treatment, and the specific method comprises the following steps: preparing a gibberellin solution, wherein the concentration range of gibberellin in the prepared gibberellin solution is 600 mg/kg, treating the gibberellin solution for 10min under the frequency condition of 20000Hz through a water treater, then using the gibberellin solution for cranberry seed soaking, wherein the seed soaking time is 22 hours, a cranberry seed soaking container is a plastic culture dish, and the cranberry seed soaking container is washed twice with distilled water with the pH =4.0 before being soaked.
Example 9:
the method for breaking the cranberry seed dormancy by using the exogenous hormone comprises the following steps: melatonin is added during the seed soaking treatment of the cranberry seeds, and the specific method comprises the following steps: preparing a melatonin solution, wherein the concentration range of the melatonin in the prepared melatonin solution is 400mg/kg, treating the melatonin solution for 10min under the condition of 20000Hz frequency through a water treatment device, then soaking the melatonin solution in cranberry seeds, then soaking the cranberry seeds for 22 hours, wherein a cranberry seed soaking container is a plastic culture dish, and washing the cranberry seeds twice with distilled water with the pH of =4.0 before soaking the cranberry seeds.
Example 10:
the method for breaking the dormancy of cranberry seeds by using the exogenous hormone comprises the following steps: melatonin is added during cranberry seed soaking treatment, and the specific method comprises the following steps: preparing a melatonin solution, wherein the concentration range of the melatonin in the prepared melatonin solution is 200mg/kg, treating the melatonin solution for 10min under the frequency condition of 20000Hz by a water treatment device, then soaking the melatonin solution in cranberry seeds, soaking the cranberry seeds in the cranberry seed soaking container for 22 hours, and washing the cranberry seeds twice with distilled water with the pH =4.0 before soaking the cranberry seeds in the cranberry seed soaking container.
In order to verify the effect of the invention, a confidentiality experiment was performed, specifically as follows:
experiment 1:
the experiment is used for verifying the influence of different dosages of melatonin on the cranberry seed germination rate.
1. The melatonin concentrations were 50, 100, 200, 300, 400mg/kg, respectively. Clear water is CK.
2. Preparation of exogenous hormone
(1) The pH value of the distilled water is adjusted to 4.0 for standby.
(2) 0.1000g of melatonin is respectively weighed and placed in a 100ml small beaker, 2ml of absolute ethyl alcohol is firstly used for dissolving the melatonin, then 30-50ml of distilled water is added, the melatonin is respectively transferred into a 100ml volumetric flask, the 100ml volumetric flask is washed by the distilled water, the volume is fixed to a scale mark, and the concentration of the melatonin is 1000 mg/kg at the moment.
(3) Respectively sucking 1000 mg/kg melatonin solution 0.5, 1.0, 2.0, 3.0 and 4.0ml into a 10ml volumetric flask, and fixing the volume with distilled water with pH =4.0, wherein the melatonin concentration is 50, 100, 200, 300 and 400 mg/kg;
(4) The melatonin solution is treated by a water treatment device under 20000Hz frequency for 10min before application.
3. Seed treatment
3500 full seeds harvested in the autumn of 2021 year are selected, the seeds are soaked in 0.5% sodium hypochlorite for 10 minutes for surface disinfection, and then the seeds are washed with clean water and washed twice with distilled water with the pH = 4.0; and then taking 18 plastic culture dishes with covers, putting 170-180 seeds into each culture dish, adding melatonin solution with different concentrations to soak the seeds for 22 hours, pouring off the seed soaking solution, washing the surfaces of the seeds three times by using distilled water with the pH =4.0, putting the seeds into a sterilized culture dish to accelerate germination, taking 50 seeds in each culture dish, repeating the steps for three times, and taking the distilled water with the pH =4.0 as a control (the melatonin is easy to decompose when exposed to light, and the solution preparation and seed soaking processes are carried out in a dark place).
4. Conditions for accelerating germination
Culturing in a climatic chamber under variable temperature and intermittent illumination conditions:
(1) Illumination: the illumination time is 14h/d, and the illumination intensity is 8000Lx; the dark time is 8h/d, and the illumination is 0Lx;
(2) Light quality: the red/white ratio is 1;
(3) Temperature: 14h at 25 ℃ (light phase), 8h at 20 ℃ (dark phase);
5. frequency of investigation: the survey was performed every 5 days.
6. As a result, the
TABLE 1 Effect of different concentrations of melatonin on cranberry seed germination
The results show that different dosages of melatonin have certain promotion effect on the improvement of the cranberry seed germination rate.
Experiment 2:
the experiment is used for verifying the influence of different dosages of gibberellin on the cranberry seed germination rate.
1. The gibberellin concentrations were 200, 400, 600, 800, 1000, 1200mg/kg, respectively. Clear water is CK.
2. Exogenous hormone formulation
(1) The pH value of the distilled water is adjusted to 4.0 for standby.
(2) Gibberellin is weighed and placed in a 100ml small beaker, firstly 2ml of absolute ethyl alcohol is used for dissolving, then 30-50ml of distilled water is added, the gibberellin is transferred into a 100ml volumetric flask, the volumetric flask is washed by the distilled water, the volume is determined to the scale mark, and the concentration of the gibberellin is 1000 mg/kg, 2000 mg/kg and 500 mg/kg respectively.
(3) Respectively sucking 1.0 ml, 2.0 ml, 3.0 ml, 4.0ml, 5.0 ml and 6.0ml of gibberellin solution into a 10ml volumetric flask, and fixing the volume by using distilled water with pH =4.0, wherein the gibberellin concentration is 200, 400, 600, 800, 1000 and 1200mg/kg respectively;
(4) The gibberellin solution is treated by a water treater under 20000Hz frequency for 10min before application.
3. Seed treatment
3500 full seeds harvested in the autumn of 2021 year are selected, the seeds are soaked in 0.5% sodium hypochlorite for 10 minutes for surface disinfection, and then the seeds are washed with clean water and washed twice with distilled water with the pH = 4.0; and then taking 18 plastic culture dishes with covers, putting 170-180 seeds in each culture dish, sequentially adding gibberellin solutions with different concentrations to soak the seeds for 22h, pouring out the seed soaking solution, washing the surfaces of the seeds three times by using distilled water with pH =4.0, putting the seeds in the sterilized culture dishes to accelerate germination, taking 50 seeds in each culture dish, repeating the steps for three times, and taking distilled water with pH =4.0 as a control (gibberellin is carried out under normal illumination conditions).
4. Conditions for accelerating germination
Culturing in a climatic chamber under variable temperature and intermittent illumination conditions:
(1) Illumination: the illumination time is 14h/d, and the illumination intensity is 8000Lx; the dark time is 8h/d, and the illumination is 0Lx;
(2) Light quality: a red/white ratio of 1;
(3) Temperature: 14h at 25 ℃ (light phase), 8h at 20 ℃ (dark phase);
5. frequency of investigation: the survey was performed every 5 days.
6. Results
TABLE 2 Effect of different concentrations of gibberellins on cranberry seed germination
The result shows that different dosages of gibberellin have certain promotion effect on the improvement of the cranberry seed germination rate.
Experiment 3:
the experiment is used for verifying the influence of different dosages of kinetin on the cranberry seed germination rate.
1. The concentration of kinetin is 5, 10, 20, 40, 80 and 160 mg/kg respectively. Clear water is CK.
2. Exogenous hormone formulation
(1) The pH value of the distilled water is adjusted to 4.0 for standby.
(2) 0.05g of kinetin is respectively weighed and placed in a 100ml small beaker, firstly 2ml of absolute ethyl alcohol is used for dissolving the kinetin, then 30-50ml of distilled water is added, the kinetin is transferred into a 100ml volumetric flask, the solution is washed by the distilled water and the volume is determined to the scale mark, and at the moment, the concentration of the kinetin is respectively 500 mg/kg.
(3) 0.1, 0.2, 0.4, 0.8, 1.6 and 3.2ml of kinetin with the concentration of 500 mg/kg are respectively sucked into a volumetric flask with 100ml, and the volume is determined by distilled water with the pH =4.0, wherein the kinetin concentration is respectively 5, 10, 20, 40, 80 and 160 mg/kg.
(4) Treating with water treater at 20000Hz frequency for 10min before application.
3. Seed treatment
3500 full seeds harvested in the autumn of 2021 are selected, the seeds are soaked in 0.5 percent sodium hypochlorite for 10 minutes for surface disinfection, and then are washed by clean water and then washed twice by distilled water with the pH = 4.0; and then taking 18 plastic culture dishes with covers, putting 170-180 seeds into each culture dish, sequentially adding kinetin solutions with different concentrations to soak the seeds for 22 hours, pouring off the seed soaking liquid, washing the surfaces of the seeds three times by using distilled water with pH =4.0, putting the seeds into the sterilized culture dishes for accelerating germination, taking 50 seeds in each culture dish, repeating the steps for three times, and taking distilled water with pH =4.0 as a control (kinetin is carried out under normal illumination conditions).
4. Conditions for accelerating germination
Culturing in a climatic chamber under variable temperature and intermittent illumination conditions:
(1) Illumination: the illumination time is 14h/d, and the illumination intensity is 8000Lx; the dark time is 8h/d, and the illumination is 0Lx;
(2) Light quality: a red/white ratio of 1;
(3) Temperature: 14h at 25 ℃ (light phase), 8h at 20 ℃ (dark phase);
5. frequency of investigation: the survey was performed every 5 days.
6. As a result, the
TABLE 3 Effect of different concentrations of kinetin on cranberry seed germination Rate
The results show that different dosages of kinetin have certain promotion effect on the improvement of the cranberry seed germination rate.
Therefore, melatonin, gibberellin and kinetin can break the dormancy of the cranberry seeds and improve the germination rate of the cranberry seeds.
Claims (3)
1. A method for breaking cranberry seed dormancy by exogenous hormone is characterized in that: when cranberry seeds are soaked, adding hormone into water for soaking, preparing a hormone solution, and soaking the cranberry seeds in the hormone solution; the hormone is melatonin, the concentration range of the melatonin in the prepared melatonin solution is 300-400mg/kg, the melatonin solution is treated for 10min under the frequency condition of 20000Hz by a water treatment device, and then cranberry seeds are soaked; the cranberry seeds were rinsed twice with pH =4.0 distilled water prior to seed soaking.
2. The method of breaking cranberry seed dormancy by an exogenous hormone of claim 1, wherein: the cranberry seed soaking time is 22h.
3. The method of breaking cranberry seed dormancy with an exogenous hormone of claim 2, wherein: the cranberry seed soaking container is a plastic culture dish.
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| CN108029280A (en) * | 2018-01-17 | 2018-05-15 | 安徽省华禾种业有限公司 | A kind of processing method before eggplant seed sowing |
| CN108719299A (en) * | 2018-06-28 | 2018-11-02 | 陕西中医药大学 | A kind of promotion astragalus membranaceus seed germinant and preparation method thereof |
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| CN104350836A (en) * | 2014-11-11 | 2015-02-18 | 黑龙江八一农垦大学 | New application of melatonin to improving saline-alkaline stress resistance of plant seeds |
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| CN108029280A (en) * | 2018-01-17 | 2018-05-15 | 安徽省华禾种业有限公司 | A kind of processing method before eggplant seed sowing |
| CN108719299A (en) * | 2018-06-28 | 2018-11-02 | 陕西中医药大学 | A kind of promotion astragalus membranaceus seed germinant and preparation method thereof |
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