CN115529889A - Seed treatment method for accelerating germination of sedum alfredii seeds - Google Patents
Seed treatment method for accelerating germination of sedum alfredii seeds Download PDFInfo
- Publication number
- CN115529889A CN115529889A CN202210999953.8A CN202210999953A CN115529889A CN 115529889 A CN115529889 A CN 115529889A CN 202210999953 A CN202210999953 A CN 202210999953A CN 115529889 A CN115529889 A CN 115529889A
- Authority
- CN
- China
- Prior art keywords
- seeds
- sedum alfredii
- magnetic field
- temperature
- germination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides a seed treatment method for accelerating germination of sedum alfredii seeds, wherein sedum alfredii seeds stored at normal temperature are selected, are subjected to ultrasonic germination acceleration firstly, are subjected to germination acceleration under a low-temperature variable magnetic field environment, are subjected to high-temperature hormone germination acceleration, and are subjected to normal-temperature high-intensity magnetic field germination acceleration finally, the sedum alfredii treated by the method has the germination rate of about 78% in 7 days of sowing, has the germination rate of about 85% in 14 days of sowing, has the germination rate of about 90% in 21 days of sowing, has the germination rate of about 95% in 28 days of sowing, and has the germination rate of about 98% in 35 days of sowing, so that the germination rate of the sedum alfredii seeds can be effectively improved, the dormancy period of the sedum alfredii seeds can be effectively improved, and the remediation requirement of heavy metal polluted soil increasingly in China can be met.
Description
Technical Field
The invention relates to the technical field of plant cultivation, in particular to a seed treatment method for accelerating germination of sedum alfredii seeds when sedum alfredii seeds are bred and planted.
Background
Heavy metals and compounds thereof in soil are stable in properties and are not easy to degrade, and physical and chemical methods such as a soil dumping method, a dilution method, a leaching method, a physical separation method and the like are adopted, so that the cost is high, the management is difficult, secondary pollution is easy to cause, and the disturbance to the environment is large. Heavy metal phytoremediation refers to the process of removing, decomposing or containment of heavy metals in contaminated land by directly utilizing plants. At present, the method for removing the land polluted by the heavy metal by using a phytoremediation method is generally considered to be an economic and environment-friendly bioremediation technology. At present, over-enriched plants discovered worldwide have over 400 varieties, but most of the over-enriched plants grow slowly and have small enrichment amount, so that the wide application of plant restoration is restricted.
Rhodiola sachalinensis (D.Don.), (Sedum alfrediiHance) is a plant of the genus crassulaceae, crassulaceae. Perennial herbaceous plants can be propagated asexually, have many branches, are yellow in flower color, have strong vitality and are widely distributed in China, south China and east China. The plant is a heavy metal restoration plant which is most widely applied at present, and has the advantages of large biomass, wide adaptability, perennial and multi-harvest within one year and the like. Meanwhile, the ecological plant also has the adverse habitat of barren, drought, strong light and the like, has strong ornamental value, and is an excellent plant for implementing plant restoration and ecological greening. Because the heavy metal contaminated soil area is large in China, the demand of sedum alfredii hance is increasing day by day.
The sedum alfredii hance is considered as a plant mainly based on vegetative propagation, so the sedum alfredii hance is mainly bred in a cuttage breeding mode, but the traditional cuttage breeding mode has low breeding efficiency and high breeding cost, and the requirement for repairing increasingly serious heavy metal contaminated soil in China can not be met far away. Seed breeding is of great interest because of the advantages of low seed cost, large propagation coefficient, economic and rapid production of a large number of seedlings and the like. However, the seeds of the sedum alfredii hance have small seeds and harder seed coats, and the germination rate is low under natural conditions, so that the seedling rate is low. Therefore, a seed treatment method for rapidly promoting the germination of the sedum alfredii hance seeds is found, which has important significance for the remediation of the heavy metal contaminated soil in China.
Disclosure of Invention
In summary, in order to overcome the defects of the prior art, the invention provides a seed treatment method for accelerating germination of sedum alfredii hance seeds, which comprises the steps of carrying out ultrasonic germination acceleration on sedum alfredii hance seeds stored at normal temperature, then carrying out germination acceleration treatment under a low-temperature variable magnetic field environment, then carrying out high-temperature hormone germination acceleration treatment, and finally carrying out normal-temperature high-intensity magnetic field germination acceleration treatment.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a seed treatment method for accelerating germination of sedum alfredii hance seeds comprises the following steps: the method comprises the following process steps:
(1) Seed sorting
Selecting the sedum alfredii seeds stored at normal temperature, removing impurities, undeveloped seeds and damaged seeds in the sedum alfredii seeds, and then putting the selected sedum alfredii seeds into a container;
(2) Ultrasonic germination acceleration
Putting the container filled with the sedum alfredii seeds in the step (1) into an ultrasonic instrument with the temperature of 35 to 40 ℃, the ultrasonic power of 80 to 100W and the ultrasonic frequency of 45 to 50Hz for ultrasonic germination acceleration for 10 to 15 minutes,
(3) Low-temp. variable magnetic field germination accelerating
Putting the container filled with the sedum alfredii seeds in the step (2) into a magnetic field auxiliary refrigerating box, setting the temperature of the magnetic field auxiliary refrigerating box to be 3-5 ℃, turning off an illuminating lamp in the magnetic field auxiliary refrigerating box, setting the magnetic field auxiliary refrigerating box to be a frequency conversion mode, setting the magnetic field intensity of the magnetic field auxiliary refrigerating box to be 1mT, and accelerating germination of the sedum alfredii seeds for 2-3 hours under the magnetic field intensity of 1 mT; then setting the magnetic field intensity of the magnetic field auxiliary refrigerating box to be 3mT, and accelerating germination of the sedum alfredii seeds for 4 to 5 hours under the magnetic field intensity of 3 mT; finally setting the magnetic field intensity of the magnetic field auxiliary refrigerating box to be 5mT, accelerating germination of the Sedum alfredii Hance seeds for 6 to 8 hours under the magnetic field intensity of 5mT,
(4) High temperature hormone germination acceleration
Taking the container filled with the sedum alfredii seeds in the step (3) out of the magnetic field assisted refrigeration box, adding a hormone solution into the container, uniformly stirring, putting the container into an illumination incubator, and performing high-temperature culture on the sedum alfredii seeds in a dark environment, wherein the culture temperature is 50-55 ℃;
(5) Normal temp. strong magnetic field germination accelerating
Taking the container filled with the sedum alfredii seeds in the step (4) out of the illumination incubator, then putting the container into a magnetic field catalysis constant temperature and humidity chamber, keeping a dark environment in the magnetic field catalysis constant temperature and humidity chamber, and accelerating germination of the sedum alfredii seeds under normal temperature, dark and strong magnetic field environments;
(6) Cleaning and disinfecting
Taking the container filled with the sedum alfredii seeds in the step (5) out of the magnetization catalysis constant-temperature and constant-humidity box, taking the sedum alfredii seeds out of the container, draining, soaking the sedum alfredii seeds in a potassium permanganate solution for disinfection, taking the sedum alfredii seeds out of the potassium permanganate solution after disinfection, putting the sedum alfredii seeds into distilled water for washing, and draining, so that the sedum alfredii seeds are treated;
(7) Seeding and sowing
And (5) sowing the sedum alfredii seeds processed in the step (6) in a planting field or a seedling bed.
The technical scheme of the invention can also be realized as follows: when the seeds are selected in the step (1), the sedum alfredii seeds stored at normal temperature are placed into water, the weight to water volume ratio of the sedum alfredii seeds is 1g.
The technical scheme of the invention can also be realized as follows: when the high-temperature hormone is used for accelerating germination in the step (4), the hormone solution is a mixed solution of lanthanum nitrate and melatonin, wherein the lanthanum nitrate and the melatonin contain an organic silicon assistant, the volume molar concentration of the lanthanum nitrate in the mixed solution is 0.2-0.4 mol/L, the volume molar concentration of the melatonin is 10-15 umol/L, the volume percentage of the organic silicon assistant to the mixed solution is 0.05-0.1%, and the volume ratio of the weight of the Sedum alfredii Hance seed to the hormone solution is 1g.
The technical scheme of the invention can also be realized as follows: and (4) during high-temperature hormone germination acceleration, putting the container filled with the sedum alfredii seeds and the hormone solution into an illumination incubator, turning off an illuminating lamp in the illumination incubator, setting the temperature of the illumination incubator to be 50-55 ℃, and accelerating germination with the high-temperature hormone for 8-10 hours.
The technical scheme of the invention can also be realized as follows: and (5) when germination is performed in a normal-temperature high-intensity magnetic field, putting a container filled with the sedum alfredii seeds and the paclobutrazol solution into a magnetic-field-catalysis constant-temperature and constant-humidity box, then turning off an internal illuminating lamp of the magnetic-field-catalysis constant-temperature and constant-humidity box, setting the temperature of the magnetic-field-catalysis constant-temperature and constant-humidity box to be 25-30 ℃, setting the magnetic field intensity to be 5-7 mT, and setting the germination time to be 6-8 hours in the normal-temperature high-intensity magnetic field.
The technical scheme of the invention can also be realized as follows: and (3) during cleaning and disinfection in the step (6), the concentration of the potassium permanganate solution is 0.1% -0.2%, the sedum alfredii seeds treated in the step (5) are soaked in the potassium permanganate solution for disinfection for at least 3 minutes, and the disinfected sedum alfredii seeds are cleaned for 3-5 times by using distilled water.
The technical scheme of the invention can also be realized as follows: and (7) during sowing, if the sedum alfredii seeds treated in the step (6) cannot be sown in time and need to be stored, keeping the temperature of the storage environment less than or equal to 25 ℃ and keeping ventilation, and keeping the storage time less than or equal to 3 days.
The invention has the beneficial effects that:
1. the method comprises the steps of carrying out ultrasonic germination acceleration on sedum alfredii seeds stored at normal temperature, carrying out germination acceleration treatment under the environment of a low-temperature variable magnetic field, carrying out high-temperature hormone germination acceleration treatment, and carrying out normal-temperature strong magnetic field germination acceleration treatment. The sedum alfredii seeds subjected to germination accelerating treatment have the advantages that the seed germination rate is obviously improved, the seed germination period is obviously shortened, the seedling rate of the sedum alfredii seeds can be effectively improved, the production period of sedum alfredii can be obviously shortened, the sedum alfredii breeding cost is reduced, the sedum alfredii breeding efficiency is improved, and the increasingly serious remediation requirement of heavy metal contaminated soil in China is met.
2. In the ultrasonic germination acceleration in the step (2), the seeds of the sedum alfredii are subjected to ultrasonic germination acceleration for 10 to 15 minutes by adopting an ultrasonic instrument with the ultrasonic power of 80 to 100W and the ultrasonic frequency of 45 to 50Hz under the temperature condition of 35 to 40 ℃, and the seeds of the sedum alfredii are promoted to germinate by ultrasonic germination acceleration.
3. In the step (3), after a container filled with the sedum alfredii seeds is placed in a magnetic field auxiliary refrigerating box in the low-temperature variable-magnetic-field germination process, the sedum alfredii seeds are firstly germinated for 2 to 3 hours under the magnetic field strength of 1mT in a low-temperature, dark and variable-frequency mode, then the sedum alfredii seeds are germinated for 4 to 5 hours under the magnetic field strength of 3mT, finally the sedum alfredii seeds are germinated for 6 to 8 hours under the magnetic field strength of 5mT, the magnetic field strength is increased from 1mT to 5mT, and the sedum alfredii seeds are subjected to germination acceleration by using a variable magnetic field, so that the sedum alfredii seeds are promoted to germinate.
4. The method has simple steps and is quick and convenient to operate, the germination rate of the sedum alfredii seeds treated by the method can reach about 78% in 7 days of sowing, about 85% in 14 days of sowing, about 90% in 21 days of sowing, about 95% in 28 days of sowing and about 98% in 35 days of sowing, so that the germination rate of the sedum alfredii seeds can be effectively improved, the dormancy period of the sedum alfredii seeds can be shortened, and the devices such as a magnetic field auxiliary refrigerating box, an illumination incubator, a magnetic field catalysis constant-temperature constant-humidity box and the like are common devices in the field, so that the method has low treatment cost for the sedum alfredii seeds, can effectively reduce the germination acceleration cost of the sedum alfredii seeds and shorten the germination acceleration time.
Detailed Description
The present invention will be described in further detail with reference to examples.
The first embodiment is as follows:
a seed treatment method for accelerating germination of sedum alfredii seeds comprises the following steps: the method comprises the following process steps:
(1) And sorting seeds
Selecting the sedum alfredii seeds stored at normal temperature, removing impurities, underdeveloped seeds and damaged seeds in the sedum alfredii seeds, and then putting the selected sedum alfredii seeds into a container;
when the seeds are selected, the sedum alfredii seeds stored at normal temperature are placed into water, the weight ratio of the sedum alfredii seeds to the volume of the water is 1g to 3mL, the sedum alfredii seeds are stirred uniformly and then stand, impurities floating on the water surface, underdeveloped seeds and seeds suspended in the water are removed, and then the sedum alfredii seeds precipitated at the bottom of a container are taken out, drained and placed into the container.
(2) Ultrasonic germination acceleration
Putting the container filled with the sedum alfredii seeds in the step (1) into an ultrasonic instrument with the temperature of 35 ℃, the ultrasonic power of 80W and the ultrasonic frequency of 45Hz for ultrasonic germination acceleration for 10 minutes,
(3) Low-temp. variable magnetic field germination accelerating
Putting the container filled with the sedum alfredii seeds in the step (2) into a magnetic field auxiliary refrigerating box, setting the temperature of the magnetic field auxiliary refrigerating box to be 3-5 ℃, turning off an illuminating lamp in the magnetic field auxiliary refrigerating box, setting the magnetic field auxiliary refrigerating box to be in a frequency conversion mode, setting the magnetic field intensity of the magnetic field auxiliary refrigerating box to be 1mT, and accelerating germination of the sedum alfredii seeds for 2 hours under the magnetic field intensity of 1 mT; then setting the magnetic field intensity of the magnetic field auxiliary refrigerating box to be 3mT, and accelerating germination of the sedum alfredii seeds for 4 hours under the magnetic field intensity of 3 mT; finally setting the magnetic field intensity of the magnetic field auxiliary refrigerating box to 5mT, accelerating germination of the seeds of the sedum alfredii hance for 6 hours under the magnetic field intensity of 5mT,
(4) High temperature hormone germination acceleration
And (3) taking the container filled with the sedum alfredii seeds in the step (3) out of the magnetic field assisted refrigerating box, adding the hormone solution into the container, uniformly stirring, putting the container into an illumination incubator, turning off an illuminating lamp in the illumination incubator, setting the temperature of the illumination incubator to be 50-55 ℃, and accelerating germination by using the high-temperature hormone for 8 hours.
The hormone solution is a mixed solution of lanthanum nitrate and melatonin, wherein the lanthanum nitrate and melatonin contain an organic silicon assistant, the volume molar concentration of the lanthanum nitrate in the mixed solution is 0.2-0.4 mol/L, the volume molar concentration of the melatonin is 10-15 umol/L, the volume percentage of the organic silicon assistant to the mixed solution is 0.05-0.1%, and the volume ratio of the weight of the rhodiola sachalinensis seeds to the volume of the hormone solution is 1g.
(5) Normal temp. strong magnetic field germination hastening
Taking the container filled with the sedum alfredii seeds in the step (4) out of the illumination incubator, then putting the container into a magnetic field catalysis constant temperature and humidity chamber, turning off an internal illuminating lamp of the magnetic field catalysis constant temperature and humidity chamber, setting the temperature of the magnetic field catalysis constant temperature and humidity chamber to be 25 ℃, setting the magnetic field intensity to be 5mT, and setting the normal-temperature strong magnetic field germination accelerating time to be 6 hours;
(6) Cleaning and disinfecting
Taking out the container filled with the sedum alfredii seeds in the step (5) from a magnetization catalysis constant temperature and humidity box, taking out the sedum alfredii seeds from the container, draining off water, then soaking the sedum alfredii seeds in a potassium permanganate solution with the concentration of 0.1% for disinfection treatment for 3 minutes, taking out the sedum alfredii seeds from the potassium permanganate solution after the disinfection is finished, putting the sedum alfredii seeds into distilled water for washing for 3 to 5 times, and draining off the water, thus finishing the treatment of the sedum alfredii seeds;
(7) Seeding and sowing
And (5) sowing the sedum alfredii seeds processed in the step (6) in a planting field or a seedling bed.
Example two:
a seed treatment method for accelerating germination of sedum alfredii seeds comprises the following steps: the method comprises the following process steps:
(1) Seed sorting
Selecting the sedum alfredii seeds stored at normal temperature, removing impurities, underdeveloped seeds and damaged seeds in the sedum alfredii seeds, and then putting the selected sedum alfredii seeds into a container;
when the seed is selected, the sedum alfredii seed that preserves at normal temperature is put into aqueous, and the weight of sedum alfredii seed and the volume ratio of water are 1g 3mL to standing after the stirring, getting rid of the water surface floating impurity, the seed that does not develop completely and the seed of suspension in aqueous, later, taking out the sedum alfredii seed that deposits in the container bottom, and the dripdry is put into the container.
(2) Ultrasonic germination acceleration
Putting the container filled with the sedum alfredii seeds in the step (1) into an ultrasonic instrument with the temperature of 40 ℃, the ultrasonic power of 100W and the ultrasonic frequency of 50Hz for ultrasonic germination acceleration for 15 minutes,
(3) Low-temp. variable magnetic field germination accelerating
Putting the container filled with the sedum alfredii seeds in the step (2) into a magnetic field auxiliary refrigerating box, setting the temperature of the magnetic field auxiliary refrigerating box to be 3-5 ℃, turning off an illuminating lamp in the magnetic field auxiliary refrigerating box, setting the magnetic field auxiliary refrigerating box to be in a frequency conversion mode, setting the magnetic field intensity of the magnetic field auxiliary refrigerating box to be 1mT, and accelerating germination of the sedum alfredii seeds for 3 hours under the magnetic field intensity of 1 mT; then setting the magnetic field intensity of the magnetic field auxiliary refrigerating box to be 3mT, and accelerating germination of the sedum alfredii seeds for 5 hours under the magnetic field intensity of 3 mT; finally setting the magnetic field intensity of the magnetic field auxiliary refrigerating box to 5mT, accelerating germination of the seeds of the sedum alfredii hance for 8 hours under the magnetic field intensity of 5mT,
(4) High temperature hormone germination acceleration
And (4) taking the container filled with the sedum alfredii seeds in the step (3) out of the magnetic field assisted refrigerating box, then adding a hormone solution into the container, uniformly stirring, putting the container into an illumination incubator, turning off an illuminating lamp in the illumination incubator, setting the temperature of the illumination incubator to be 50-55 ℃, and accelerating germination by using high-temperature hormone for 8 hours.
The hormone solution is a mixed solution of lanthanum nitrate and melatonin, wherein the lanthanum nitrate and melatonin contain an organic silicon assistant, the volume molar concentration of the lanthanum nitrate in the mixed solution is 0.2-0.4 mol/L, the volume molar concentration of the melatonin is 10-15 umol/L, the volume percentage of the organic silicon assistant to the mixed solution is 0.05-0.1%, and the volume ratio of the weight of the rhodiola sachalinensis seeds to the volume of the hormone solution is 1g.
(5) Normal temp. strong magnetic field germination hastening
Taking the container filled with the sedum alfredii seeds in the step (4) out of the illumination incubator, then putting the container into a magnetic field catalysis constant temperature and humidity chamber, turning off an internal illuminating lamp of the magnetic field catalysis constant temperature and humidity chamber, setting the temperature of the magnetic field catalysis constant temperature and humidity chamber to be 25 ℃, setting the magnetic field intensity to be 7 mT, and setting the normal-temperature strong magnetic field germination accelerating time to be 8 hours;
(6) Cleaning and disinfecting
Taking the container filled with the sedum alfredii seeds in the step (5) out of the magnetization catalysis constant-temperature and constant-humidity box, taking the sedum alfredii seeds out of the container, draining off water, soaking the sedum alfredii seeds in a potassium permanganate solution with the concentration of 0.2% for disinfection treatment for 3 minutes, taking the sedum alfredii seeds out of the potassium permanganate solution after the disinfection treatment is finished, putting the sedum alfredii seeds into distilled water for washing for 5 times, and draining off the water, thus finishing the treatment of the sedum alfredii seeds;
(7) Seeding and sowing
And (4) sowing the sedum alfredii seeds treated in the step (6) in a planting field or a seedling bed.
Example three:
a seed treatment method for accelerating germination of sedum alfredii seeds comprises the following steps: the method comprises the following process steps:
(1) Seed sorting
Selecting the sedum alfredii seeds stored at normal temperature, removing impurities, underdeveloped seeds and damaged seeds in the sedum alfredii seeds, and then putting the selected sedum alfredii seeds into a container;
when the seed is selected, the sedum alfredii seed that preserves at normal temperature is put into aqueous, and the weight of sedum alfredii seed and the volume ratio of water are 1g 3mL to standing after the stirring, getting rid of the water surface floating impurity, the seed that does not develop completely and the seed of suspension in aqueous, later, taking out the sedum alfredii seed that deposits in the container bottom, and the dripdry is put into the container.
(2) Ultrasonic germination acceleration
Putting the container filled with the sedum alfredii seeds in the step (1) into an ultrasonic instrument with the temperature of 40 ℃, the ultrasonic power of 100W and the ultrasonic frequency of 50Hz for ultrasonic germination acceleration for 12 minutes,
(3) Low temp. variable magnetic field germination accelerating
Putting the container filled with the sedum alfredii seeds in the step (2) into a magnetic field auxiliary refrigerating box, setting the temperature of the magnetic field auxiliary refrigerating box to be 3-5 ℃, turning off an illuminating lamp in the magnetic field auxiliary refrigerating box, setting the magnetic field auxiliary refrigerating box to be in a frequency conversion mode, setting the magnetic field intensity of the magnetic field auxiliary refrigerating box to be 1mT, and accelerating germination of the sedum alfredii seeds for 2.5 hours under the magnetic field intensity of 1 mT; then setting the magnetic field intensity of the magnetic field auxiliary refrigerating box to be 3mT, and accelerating germination of the seeds of the sedum alfredii hance for 4.5 hours under the magnetic field intensity of 3 mT; finally setting the magnetic field intensity of the magnetic field auxiliary refrigerating box to 5mT, accelerating germination of the seeds of the sedum alfredii hance for 7 hours under the magnetic field intensity of 6 mT,
(4) High temperature hormone germination acceleration
And (3) taking the container filled with the sedum alfredii seeds in the step (3) out of the magnetic field assisted refrigerating box, adding the hormone solution into the container, uniformly stirring, putting the container into an illumination incubator, turning off an illuminating lamp in the illumination incubator, setting the temperature of the illumination incubator to be 50-55 ℃, and accelerating germination by using the high-temperature hormone for 8 hours.
The hormone solution is a mixed solution of lanthanum nitrate and melatonin, wherein the lanthanum nitrate and melatonin contain an organic silicon assistant, the volume molar concentration of the lanthanum nitrate in the mixed solution is 0.2-0.4 mol/L, the volume molar concentration of the melatonin is 10-15 umol/L, the volume percentage of the organic silicon assistant to the mixed solution is 0.05-0.1%, and the volume ratio of the weight of the rhodiola sachalinensis seeds to the volume of the hormone solution is 1g.
(5) Normal temp. strong magnetic field germination accelerating
Taking the container filled with the sedum alfredii seeds in the step (4) out of the illumination incubator, then putting the container into a magnetic field catalysis constant temperature and humidity chamber, turning off an internal illuminating lamp of the magnetic field catalysis constant temperature and humidity chamber, setting the temperature of the magnetic field catalysis constant temperature and humidity chamber to be 30 ℃, setting the magnetic field intensity to be 6 mT, and setting the normal-temperature strong magnetic field germination accelerating time to be 7 hours;
(6) Cleaning and disinfecting
Taking the container filled with the sedum alfredii seeds in the step (5) out of the magnetization catalysis constant-temperature constant-humidity box, taking the sedum alfredii seeds out of the container, draining off water, soaking the sedum alfredii seeds in a potassium permanganate solution with the concentration of 0.2% for disinfection treatment for 3 minutes, taking the sedum alfredii seeds out of the potassium permanganate solution after disinfection, putting the sedum alfredii seeds into distilled water for washing for 5 times, and draining off water, thus finishing the treatment of the sedum alfredii seeds;
(7) And seeding
And (4) sowing the sedum alfredii seeds treated in the step (6) in a planting field or a seedling bed.
Comparative example one:
soaking the seeds of Sedum alfredii Hance dried and stored at normal temperature (30 deg.C) in distilled water for 24 hr, washing the seeds with distilled water for 3-5 times, removing impurities, washing, and draining.
Comparative example two:
soaking seeds of Sedum alfredii Hance stored at room temperature in distilled water at 30 deg.C for 24 hr, sterilizing in 0.1% potassium permanganate solution for 3 min, cleaning with distilled water for 3-5 times, removing impurities, cleaning, and draining.
Germination experiments are carried out on the sedum alfredii seeds treated in the first embodiment, the second embodiment, the third embodiment and the first comparative embodiment and the second comparative embodiment, and the germination rate of the treated sedum alfredii seeds is counted, wherein the specific operation steps are as follows:
10 petri dishes were prepared and two layers of filter paper were laid in each petri dish. Taking 2 culture dishes paved with two layers of filter paper, and putting 50 sedum alfredii seeds treated according to the first embodiment into each culture dish. And 2 culture dishes containing the sedum alfredii seeds treated in the first embodiment are distinguished.
And taking 2 culture dishes paved with two layers of filter paper, and putting 50 sedum alfredii seeds treated in the second embodiment into each culture dish. And 2 culture dishes filled with the sedum alfredii seeds treated in the second embodiment are distinguished and identified.
And taking 2 culture dishes paved with two layers of filter paper, and putting 50 sedum alfredii seeds treated in the third embodiment into each culture dish. And 2 culture dishes containing the sedum alfredii seeds treated in the third embodiment are distinguished and identified.
And taking 2 culture dishes paved with two layers of filter paper, and putting 50 sedum alfredii seeds treated by the first control example into each culture dish. And 2 petri dishes containing the sedum alfredii seeds treated in the first comparative example were differentially labeled.
In the last 2 dishes spread with two layers of filter paper, 50 seeds of sedum alfredii treated in the second control example were placed in each dish. And 2 petri dishes filled with the sedum alfredii seeds treated in the second comparative example are distinguished and identified.
And (3) placing 10 culture dishes in a dark illumination incubator at 25 ℃ for germination, observing the moisture condition in the culture dishes every day, replenishing moisture in time, and counting the germination number every 7 days. Counting to the end of 35 days to obtain the germination numbers of the five treated seeds in 7 days, 14 days, 21 days, 28 days and 35 days respectively. The calculated germination rates are shown in table 1.
TABLE 1
As can be seen from table 1:
(1) The germination rates of the sedum alfredii seeds treated in the first comparative example and the sedum alfredii seeds treated in the second comparative example are only 44.87% and 69.23% when the sedum alfredii seeds treated in the first comparative example are cultured and germinated for 35 days, the germination rate of the sedum alfredii seeds treated in the second example reaches 98.23%, the germination rate of the sedum alfredii seeds treated in the second example can reach 97.76% when the sedum alfredii seeds treated in the second example are cultured and germinated for 35 days, and the germination rate of the sedum alfredii seeds treated in the third example can reach 96.95%, so that the germination rate of the sedum alfredii seeds can be effectively improved.
(2) The germination rates of the sedum alfredii seeds treated in the first and second control examples are only 23.08% and 44.87% when the sedum alfredii seeds treated in the first embodiment are cultured and germinated for 7 days, while the germination rates of the sedum alfredii seeds treated in the first embodiment are 76.53%, the germination rates of the sedum alfredii seeds treated in the second embodiment are 78.12% when the sedum alfredii seeds treated in the second embodiment are cultured and germinated for 7 days, and the germination rates of the sedum alfredii seeds treated in the third embodiment are 77.2%, namely, the germination rates of the sedum alfredii seeds treated by the method can reach about 78% when the sedum alfredii seeds treated by the method are cultured and germinated for 7 days, and are higher than the germination rates of the sedum alfredii seeds treated in the first and second control embodiments for 35 days, so that the method can effectively shorten the dormancy time of the sedum alfredii seeds.
It should be noted that the above-mentioned embodiments illustrate rather than limit the technical solutions of the present invention, and that equivalent substitutions or other modifications made by those skilled in the art according to the prior art are intended to be included within the scope of the claims of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (7)
1. A seed treatment method for accelerating germination of sedum alfredii seeds is characterized by comprising the following steps: the method comprises the following process steps:
(1) And sorting seeds
Selecting the sedum alfredii seeds stored at normal temperature, removing impurities, underdeveloped seeds and damaged seeds in the sedum alfredii seeds, and then putting the selected sedum alfredii seeds into a container;
(2) Ultrasonic germination acceleration
Putting the container filled with the sedum alfredii seeds in the step (1) into an ultrasonic instrument with the temperature of 35 to 40 ℃, the ultrasonic power of 80 to 100W and the ultrasonic frequency of 45 to 50Hz for ultrasonic germination acceleration for 10 to 15 minutes,
(3) Low-temp. variable magnetic field germination accelerating
Putting the container filled with the sedum alfredii seeds in the step (2) into a magnetic field auxiliary refrigerating box, setting the temperature of the magnetic field auxiliary refrigerating box to be 3-5 ℃, turning off an illuminating lamp in the magnetic field auxiliary refrigerating box, setting the magnetic field auxiliary refrigerating box to be a frequency conversion mode, setting the magnetic field intensity of the magnetic field auxiliary refrigerating box to be 1mT, and accelerating germination of the sedum alfredii seeds for 2-3 hours under the magnetic field intensity of 1 mT; then setting the magnetic field intensity of the magnetic field auxiliary refrigerating box to be 3mT, and accelerating germination of the Sedum alfredii Hance seeds for 4 to 5 hours under the magnetic field intensity of 3 mT; finally setting the magnetic field intensity of the magnetic field auxiliary refrigerating box to 5mT, accelerating germination of the seeds of the sedum alfredii hance for 6 to 8 hours under the magnetic field intensity of 5mT,
(4) High temperature hormone germination acceleration
Taking the container filled with the sedum alfredii seeds in the step (3) out of the magnetic field assisted refrigeration box, adding a hormone solution into the container, uniformly stirring, putting the container into an illumination incubator, and performing high-temperature culture on the sedum alfredii seeds in a dark environment, wherein the culture temperature is 50-55 ℃;
(5) Normal temp. strong magnetic field germination hastening
Taking the container filled with the sedum alfredii seeds in the step (4) out of the illumination incubator, then putting the container into a magnetic field catalysis constant temperature and humidity chamber, keeping a dark environment in the magnetic field catalysis constant temperature and humidity chamber, and accelerating germination of the sedum alfredii seeds under normal temperature, dark and strong magnetic field environments;
(6) Cleaning and disinfecting
Taking the container filled with the sedum alfredii seeds in the step (5) out of the magnetization catalysis constant-temperature constant-humidity box, taking the sedum alfredii seeds out of the container, draining, soaking the sedum alfredii seeds in a potassium permanganate solution for disinfection treatment, taking the sedum alfredii seeds out of the potassium permanganate solution after the disinfection treatment is finished, putting the sedum alfredii seeds into distilled water for cleaning, and draining, thus finishing the treatment of the sedum alfredii seeds;
(7) Seeding and sowing
And (5) sowing the sedum alfredii seeds processed in the step (6) in a planting field or a seedling bed.
2. The seed treatment method for accelerating germination of sedum alfredii hance seeds as claimed in claim 1, wherein: and (2) when the seeds are sorted, putting the sedum alfredii seeds stored at normal temperature into water, wherein the weight ratio of the sedum alfredii seeds to the water is 1g.
3. The seed treatment method for accelerating germination of sedum alfredii hance seeds as claimed in claim 1, wherein: when the high-temperature hormone is used for accelerating germination in the step (4), the hormone solution is a mixed solution of lanthanum nitrate and melatonin, wherein the lanthanum nitrate and the melatonin contain an organic silicon assistant, the volume molar concentration of the lanthanum nitrate in the mixed solution is 0.2-0.4 mol/L, the volume molar concentration of the melatonin is 10-15 umol/L, the volume percentage of the organic silicon assistant to the mixed solution is 0.05-0.1%, and the volume ratio of the weight of the Sedum alfredii Hance seed to the hormone solution is 1g.
4. The seed treatment method for accelerating germination of sedum alfredii hance seeds as claimed in claim 3, wherein: and (4) when high-temperature hormone germination is carried out, putting the container filled with the sedum alfredii seeds and the hormone solution into an illumination incubator, turning off an illuminating lamp in the illumination incubator, setting the temperature of the illumination incubator to be 50-55 ℃, and carrying out high-temperature hormone germination for 8-10 hours.
5. The seed treatment method for accelerating germination of sedum alfredii hance seeds as claimed in claim 1, wherein: and (5) when germination is performed in a normal-temperature high-intensity magnetic field, putting a container filled with the sedum alfredii seeds and the paclobutrazol solution into a magnetic-field-catalysis constant-temperature and constant-humidity box, then turning off an internal illuminating lamp of the magnetic-field-catalysis constant-temperature and constant-humidity box, setting the temperature of the magnetic-field-catalysis constant-temperature and constant-humidity box to be 25-30 ℃, setting the magnetic field intensity to be 5-7 mT, and setting the germination time to be 6-8 hours in the normal-temperature high-intensity magnetic field.
6. The seed treatment method for accelerating germination of sedum alfredii seeds as claimed in claim 1, wherein: and (3) during cleaning and disinfection in the step (6), the concentration of the potassium permanganate solution is 0.1% -0.2%, the sedum alfredii seeds treated in the step (5) are soaked in the potassium permanganate solution for disinfection for at least 3 minutes, and the disinfected sedum alfredii seeds are cleaned for 3-5 times by using distilled water.
7. The seed treatment method for accelerating germination of sedum alfredii seeds as claimed in claim 1, wherein: and (7) during sowing, if the sedum alfredii seeds treated in the step (6) cannot be sowed in time and need to be stored, the storage environment temperature is less than or equal to 25 ℃, ventilation is kept, and the storage time is less than or equal to 3 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210999953.8A CN115529889A (en) | 2022-08-19 | 2022-08-19 | Seed treatment method for accelerating germination of sedum alfredii seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210999953.8A CN115529889A (en) | 2022-08-19 | 2022-08-19 | Seed treatment method for accelerating germination of sedum alfredii seeds |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115529889A true CN115529889A (en) | 2022-12-30 |
Family
ID=84725086
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210999953.8A Pending CN115529889A (en) | 2022-08-19 | 2022-08-19 | Seed treatment method for accelerating germination of sedum alfredii seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115529889A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20050004555A (en) * | 2003-07-03 | 2005-01-12 | 이강수 | Presown seed treatments to elevate seedling emergence of Rhodiola |
| CN101057542A (en) * | 2007-05-22 | 2007-10-24 | 浙江大学 | Sexual reproductive method of sedum alfredii |
| JP2013022016A (en) * | 2011-07-20 | 2013-02-04 | Miyazaki Mokuzaiten:Kk | Method for cultivating sedum grass |
| US20180160629A1 (en) * | 2015-05-26 | 2018-06-14 | Bruce K. Redding, Jr. | Ultrasonic treatment of seeds |
| CN112335371A (en) * | 2020-12-09 | 2021-02-09 | 河南省农业科学院经济作物研究所 | Seed treatment method for accelerating germination of polygonatum sibiricum seeds |
| CN112930751A (en) * | 2021-03-08 | 2021-06-11 | 河南省农业科学院经济作物研究所 | Seed treatment method for accelerating germination of sedum alfredii seeds |
| CN114342599A (en) * | 2022-01-24 | 2022-04-15 | 黑龙江八一农垦大学 | Method for breaking cranberry seed dormancy by exogenous hormone |
-
2022
- 2022-08-19 CN CN202210999953.8A patent/CN115529889A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20050004555A (en) * | 2003-07-03 | 2005-01-12 | 이강수 | Presown seed treatments to elevate seedling emergence of Rhodiola |
| CN101057542A (en) * | 2007-05-22 | 2007-10-24 | 浙江大学 | Sexual reproductive method of sedum alfredii |
| JP2013022016A (en) * | 2011-07-20 | 2013-02-04 | Miyazaki Mokuzaiten:Kk | Method for cultivating sedum grass |
| US20180160629A1 (en) * | 2015-05-26 | 2018-06-14 | Bruce K. Redding, Jr. | Ultrasonic treatment of seeds |
| CN112335371A (en) * | 2020-12-09 | 2021-02-09 | 河南省农业科学院经济作物研究所 | Seed treatment method for accelerating germination of polygonatum sibiricum seeds |
| CN112930751A (en) * | 2021-03-08 | 2021-06-11 | 河南省农业科学院经济作物研究所 | Seed treatment method for accelerating germination of sedum alfredii seeds |
| CN114342599A (en) * | 2022-01-24 | 2022-04-15 | 黑龙江八一农垦大学 | Method for breaking cranberry seed dormancy by exogenous hormone |
Non-Patent Citations (1)
| Title |
|---|
| 张明芹编著: "《物理》", 哈尔滨：东北林业大学出版社, pages: 136 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111226533B (en) | Method for germinating mallotus japonicus seeds and seedling culture method | |
| CN101595831B (en) | Seedlings culturing method of rare or endangered species-acer yangbiense seedlings and cultivating method thereof | |
| CN111557219A (en) | Polygonatum cyrtonema seedling growing method | |
| CN103858771B (en) | A kind of transplanting method of corn gene plantlet in vitro | |
| CN106665357A (en) | Method for establishing lycoris regeneration system | |
| CN101455179B (en) | Tissue culture method of aged Sinojackia xylocarpa | |
| CN112930751A (en) | Seed treatment method for accelerating germination of sedum alfredii seeds | |
| CN107593443B (en) | Tissue culture rapid propagation method of kaempferia galanga | |
| CN106550749B (en) | A method of enhancing bluish dogbane absorbs Cd in soil | |
| CN101622922B (en) | Symbiotic germination method of Chinese cymbidium hybrid seeds and bacteria | |
| CN101675740A (en) | Repair plant black nightshade seed-soaking solution and application thereof | |
| CN110809933A (en) | Method for rapid germination of phoenix tree seeds | |
| CN1869202A (en) | Free small spore culturing technology of non heading cabbage | |
| CN109601388B (en) | Tissue culture rapid propagation method of hybrid clematis | |
| CN111903488A (en) | Soilless culture method for orchid | |
| CN109863855A (en) | The method for improving Germination Percentage of Rice under Cd stress | |
| CN102388807A (en) | Rapid propagation method for tissue culture of wild Thymus Mongolicus Ronn and application thereof | |
| CN115529889A (en) | Seed treatment method for accelerating germination of sedum alfredii seeds | |
| CN106106145A (en) | A kind of method for in-vitro rapid propagation of snowball embryo Seedling stem section | |
| CN113317158B (en) | Seedling raising method for improving rapid seedling formation of Australian water lily seed seedlings | |
| CN105165610A (en) | High-efficiency propagation method of Zinnia elegans virus-free seedling | |
| CN112410254B (en) | Bioremediation strain for promoting plant growth and enhancing soil heavy metal extraction and remediation method | |
| CN111771726B (en) | Transplanting method of delicious kiwi fruit rootstock rootless tissue culture seedlings | |
| CN111034557B (en) | Seedling for indoor inoculation of barley yellow mosaic disease and culture method of seedling after inoculation | |
| CN113615540A (en) | Artificial composite matrix, artificially cultured eel grass seedlings, and culture method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |