CN114322797A - Optical detection cutting method and system for biological membrane tissue based on weak coherent interference - Google Patents
Optical detection cutting method and system for biological membrane tissue based on weak coherent interference Download PDFInfo
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Abstract
The invention discloses a method and a system for detecting and cutting biofilm tissues optically based on weak coherent interference. Placing a die-cutting assembly over the biological tissue, the die-cutting assembly having the biofilm tissue disposed therein; detecting the thickness of a target area of the biofilm tissue through the die cutting assembly by utilizing an optical coherence imaging system (OCT); and controlling a die in the die-cutting assembly to cut the target area to obtain the artificial heart biological valve leaflet based on the thickness of the target area of the biological membrane tissue. The invention can realize the micrometer precision and non-contact measurement of the thickness of the biological membrane tissue, is beneficial to cutting the artificial heart biological valve leaflets with uniform thickness, and can be quickly realized.
Description
Technical Field
The present invention relates to a method and system for treating a biological membrane tissue, and more particularly to a method, system and mold for evaluating and cutting a biological membrane tissue to obtain weak coherent optical detection cuts of bioprosthetic heart valve leaflets.
Background
The normal native heart includes the aortic, mitral, tricuspid, and pulmonary valves, each of which has unidirectional leaflets to control the directional flow of blood through the heart. When the heart valve is diseased or damaged, in order to work normally, the heart valve can be replaced by a heart valve prosthesis.
The artificial heart biological valve is made of biological tissue materials, such as bovine pericardium tissue and porcine pericardium tissue which are used for manufacturing single leaflets of the artificial heart biological valve. A typical procedure commonly used to prepare bioprosthetic heart valve leaflets is to take a fresh animal pericardial sac, cut the pericardial sac flat and remove excess fat and impurities. The tissue is cross-linked with the drug after trimming the apparently unusable area, followed by removal of the rough edges of the tissue.
The treated biofilm tissue is then placed on a platform and the thickness of the biofilm tissue is measured. Thickness measurements are typically made with a touch indicator, which measures the thickness at different spatial locations by moving the biofilm tissue around the platform, while the indicator needs to be moved up and down at different points to touch the biofilm tissue. A further improved thickness measurement head/platen with multiple sensors can be used to measure the thickness at multiple points, selecting the appropriate thickness area to process into leaflets. However, such contact measurements are time consuming, prone to damage to the biofilm tissue, and the quality of the resulting leaflets is susceptible.
There is an urgent need for accurate and efficient evaluation of the processing of biofilm tissue to obtain prosthetic heart valve leaflets. In addition, this need is even more important for the placement of compressible/expandable prosthetic heart valves, as uneven thickness/mismatched leaflets can affect the proper function of the prosthetic heart valve.
Disclosure of Invention
The invention aims to provide a method, a system and a mold for detecting and cutting biofilm tissues based on weak coherent interference aiming at the defects of the prior art.
The purpose of the invention is realized by the following technical scheme:
a method for detecting and cutting a biological membrane tissue optically based on weak coherent interference comprises the following steps:
placing a die-cutting assembly over the biological tissue, the die-cutting assembly having the biofilm tissue disposed therein;
detecting the thickness of a target area of the biofilm tissue through the die cutting assembly by utilizing an optical coherence imaging system (OCT);
and controlling a die in the die-cutting assembly to cut the target area to obtain the artificial heart biological valve leaflet based on the thickness of the target area of the biological membrane tissue.
The biofilm tissue, comprising: pericardium, aortic valve, dura mater, peritoneum, septum, or intestinal submucosa, but is not limited thereto.
The optical coherence imaging system OCT is a weak coherence optical detection system.
Detecting the thickness of the biofilm tissue through the die cutting assembly by utilizing an optical coherence imaging system (OCT); the method comprises the following steps:
acquiring an interference spectrum of the biofilm tissue by using OCT;
extracting a depth domain signal from the interference spectrum of the biofilm tissue;
the thickness measurement is performed with micron accuracy on the biofilm tissue based on the depth domain signal.
The method for acquiring the interference spectrum of the biofilm tissue by using the OCT comprises the following steps:
a time domain OCT imaging method for changing the optical path of the reference arm by scanning;
or a spectral domain OCT imaging method for recording spectral interference signals by a spectrometer;
or a frequency-sweep OCT imaging method which utilizes a frequency-sweep light source to record spectral interference signals.
Detecting a thickness of a biofilm tissue of a target area from below an optically transparent plate with an optical coherence imaging system (OCT), comprising: the OCT detection range is set according to the area of the target region, and the measurement sequence of different space points of the target region is determined by the OCT scanning sequence.
Detecting the thickness of the biofilm tissue through the die cutting assembly by utilizing an optical coherence imaging system (OCT); the method comprises the following steps: when in detection, the center of the detection range of the optical coherence imaging system OCT, the center of the target area of the biomembrane tissue and the center of the die pattern of the cutting die are kept in the same vertical straight line.
Cutting the target area with the mold to obtain a prosthetic heart bioprosthetic valve leaflet based on the OCT thickness measurement of the biofilm tissue, comprising:
laying and placing the biological membrane tissues on an optical transparent plate of a displacement table;
lifting the cutting die over the biofilm tissue by a vertical displacement device;
horizontally moving the displacement table until the target area of the biofilm tissue is positioned right below the cutting die;
measuring a target area thickness of the biofilm tissue from directly below the target area of the biofilm tissue by an optical coherence imaging system OCT;
determining whether the thickness of the target area is suitable for obtaining the prosthetic heart valve leaflet, and particularly considering whether the thickness is suitable or not;
and lowering the cutting die by a vertical displacement device to cut the biological membrane tissue of the target area to obtain the artificial heart biological valve leaflet.
The invention adopts an optical mode to detect and cut the thickness, realizes the processing and obtaining of the artificial heart biological valve leaflet in a new mode, and can overcome the defects and problems of contact measurement.
The die cutting assembly comprises a displacement table with a transparent plate, a vertical displacement device, a transparent glass table top and a cutting die; one side is equipped with vertical displacement device on the transparent glass mesa, and cutting die sets up on the transparent glass mesa, places the displacement platform that has the light transparent plate on the transparent glass mesa, and the tiling is placed the biomembrane and is organized on the displacement platform.
Specifically, the thickness of the target area of the biological membrane tissue is detected by an optical coherence imaging system (OCT) from the lower part of the optical transparent plate to the upper part through the transparent glass table top and the displacement table with the optical transparent plate.
The vertical displacement device comprises a mounting rod, a connecting arm and a height display; the bottom end of the mounting rod is fixed on the transparent glass table-board, and the height display is connected to the middle part of the mounting rod through a connecting arm; the height display extends above the biofilm tissue, and the cutting die is fixedly mounted at the bottom end of the height display, so that the cutting die is positioned above the biofilm tissue. The vertical displacement device drives the cutting die to move up and down.
The cutting die is provided with a die pattern and is used for cutting the biofilm tissue according to the die pattern.
Moving the displacement stage horizontally until a target area of biofilm tissue is directly beneath the cutting die, comprising: the horizontal displacement of the displacement table allows manual adjustment and automation system control.
The displacement table, the vertical displacement device and the cutting die are all connected to the signal processor module and the computing unit 45, and the signal processor module and the computing unit 45 control the work of the displacement table, the vertical displacement device and the cutting die. And the scanning lens 51 is located below the transparent glass mesa.
Secondly, a biomembrane tissue optical detection cutting system based on weak coherent interference comprises:
a set of die cutting components for cutting the biological membrane tissue to obtain the prosthetic heart biological valve leaflets;
the OCT optical coherence detection device is used for collecting the interference spectrum of the biofilm tissue in a two-dimensional or three-dimensional space;
and the one or more processors are coupled to the die cutting assembly and the OCT optical coherence detection device and are respectively used for connecting the die cutting assembly, controlling the die in the die cutting assembly to vertically displace and cut, receiving the OCT interference spectrum from the OCT optical coherence detection device and analyzing and processing the OCT interference spectrum to obtain the depth information of the biomembrane tissue.
The OCT optical coherence detection device adopts one of the following methods:
the system comprises a low-coherence light source, an interferometer and a detector;
or a low coherence light source, an interferometer and a spectrometer;
or a swept-bandwidth spectral light source, an interferometer and a detector.
The method comprises the steps of placing a die cutting assembly above the biological membrane tissue, wherein the die cutting assembly comprises a transparent glass table top and a cutting die with a die pattern, the die is attached to the transparent glass table top, a displacement table with a light transparent plate is placed on the transparent glass table top of the die cutting assembly, the biological membrane tissue is flatly placed on the surface of the light transparent plate in the displacement table, and the optical coherence imaging system OCT is utilized to carry out biological membrane tissue imaging from the lower part of the light transparent plate to measure the thickness of the tissue.
The die cutting assembly of the present invention can be mounted for automated vertical movement and the displacement stage on which the tissue is placed can be moved horizontally. Different target areas on the tissue can be evaluated for thickness by OCT measurements, and when the appropriate target area thickness is detected, the mold cuts the desired prosthetic heart bioprosthetic valve leaflet shape from that area. OCT can achieve precision in the thickness of the tissue of the biofilm, non-contact measurement, help to cut leaflets of bioprosthetic heart valves of uniform thickness, and can quickly accomplish this process.
Compared with the prior art, the invention has the following beneficial effects and advantages:
compared with sensor contact measurement, the invention can realize non-contact simultaneous acquisition of thickness information (micrometer precision) of a plurality of spatial position points (micrometer spacing). Meanwhile, the invention combines the optical detection system and the die cutting assembly, and can realize rapid evaluation of cutting of the biological membrane tissue to obtain the leaflet of the artificial heart biological valve.
Drawings
FIG. 1 is a schematic diagram of the process of the present invention;
FIG. 2 is a schematic view of the apparatus of the present invention;
FIG. 3 is a schematic diagram of an embodiment of the present invention;
figure 4 is a diagram of an OCT architecture according to an exemplary embodiment of the present invention.
Wherein: 1-collecting interference spectrum by using OCT; 2-extracting depth domain signals from the interference spectrum; 3-extracting the tissue thickness based on the depth domain signal; 4-selecting a target area with a proper thickness for die cutting; 11-a light source; 12-a beam splitter; 13-reference arm collimator lens; 14-plane high reflector; 15-sample arm collimator; 16-a scanning galvanometer; 17-an objective lens; 18-a sample to be tested; 19-interference signal detection means; 20-a signal processor; 21-a polarization controller; 31-low coherence broadband light source; 32-an optical circulator; 33-a fiber coupler; 34-a first fiber alignment device; 35-a focusing lens; 36-plane high mirror; 37-a second fiber alignment device; 38-scanning galvanometer; 39-a focusing lens; 41-a third fiber collimating device; 42-a grating; a 43-Fourier transform lens; 44-high speed line camera; 45-signal processor module and computing unit; 46-a first polarization controller; 47-a second polarization controller; 48: a dispersion compensator; 49: a focusing lens; 50: a dichroic mirror; 51: a scanning lens; 100: a die cutting assembly; 102: a displacement stage having an optically transparent plate; 104: a vertical displacement device; 106: biofilm tissue; 108: a transparent glass table top; 114: mounting a rod; 115: a connecting arm; 118: a height display; 120: and cutting the die.
Detailed Description
Reference will now be made in detail to the present embodiments of the invention, examples of which are illustrated in the accompanying drawings, which form a part hereof. It should be noted that the description and illustrations are exemplary only and should not be construed as limiting the scope of the invention, which is defined by the appended claims, as any variation based on the claims is intended to be within the scope of the invention.
To facilitate an understanding of embodiments of the invention, operations are described as multiple discrete operations, however, the order of description does not represent the order in which the operations are performed.
The x-y-z three-dimensional coordinate representation based on spatial direction is adopted for the sample measurement space in the description. This description is merely intended to facilitate discussion and is not intended to limit application of embodiments of the present invention. Wherein: the depth z direction is a direction along the incident optical axis; the x-y plane is a plane perpendicular to the optical axis, where x is orthogonal to y, and x denotes the OCT lateral fast scan direction and y denotes the slow scan direction.
The above I, x, y, z, etc. represent variables used merely to facilitate discussion and are not intended to limit the application of embodiments of the present invention, and can be any of values 1,2,3,4, etc.
As shown in figure 1, the method firstly utilizes OCT to collect interference spectrum, then extracts depth domain signals from the interference spectrum, extracts tissue thickness based on the depth domain signals, and finally selects a target area with proper thickness for die cutting.
The method comprises the steps of collecting interference spectrum of a biological membrane tissue by using an OCT system, carrying out OCT scanning imaging of two-dimensional or three-dimensional space on the tissue sample, repeatedly scanning and imaging for a certain time at the same spatial position, and recording a spectrum interference signal by using a spectrometer (or a time domain OCT imaging method for changing the optical path of a reference arm by scanning and a sweep frequency OCT imaging method for recording the spectrum interference signal by using a sweep frequency light source).
The OCT interference spectrum is used for extracting a depth domain signal, namely, the Fourier transform is carried out on the interference spectrum to obtain a depth domain complex signal (comprising an intensity component and a phase component) of each space point.
And extracting the upper and lower boundaries of the biological membrane tissue based on the intensity component of the depth domain signal to obtain corresponding depth position coordinates, and multiplying the coordinate interval of the two by the actual size represented by a single pixel, namely the corresponding biological membrane thickness. The method comprises the following specific steps: in the three-dimensional signal of the biofilm tissue, I represents the OCT intensity signal, and the intensity difference dI (z1, x1, y1) between the same longitudinal coordinate y1, the same transverse coordinate x1, and the adjacent depth coordinates z is calculated:
dI(z1,x1,y1)＝I(z1,x1,y1)-I(z1-1,x1,y1) (1)
at the upper and lower edge positions of the biological membrane, there will be a difference of the intensity signals of the depth domain with the adjacent image area except the biological membrane signal, the differential value dI will be significantly larger than other positions, because there are two edges, two maximum values are selected by comparison, and the corresponding depth coordinate z ', z "is obtained, and if z' > z" is assumed, then at the transverse coordinate point x1, the longitudinal coordinate point y1, and the thickness dz (x1, y1) of the biological membrane tissue is:
dz(x1,y1)＝(z′-z″)×u (2)
where u represents the actual size of a single pixel representation in the z-direction of the depth coordinate in OCT.
The thickness of the biological membrane tissue can be calculated by the method at any longitudinal coordinate position y and any transverse coordinate position x, and then a two-dimensional membrane thickness matrix dz (x, y) can be obtained. The die pattern size determines the target area size of the cut, and the average and variance of the two-dimensional film thickness matrix dz (x, y) within the target area size are calculated, traversing the entire two-dimensional film thickness matrix. The small variance represents that the thickness of the inner membrane in the area is uniform, which is beneficial to manufacturing the artificial heart biological valve leaflet with normal function. And screening out a target area with uniform film thickness according to the variance, and then selecting proper thickness according to the average value for cutting.
Fig. 2 shows a schematic view of an apparatus for treating biofilm tissue according to the present invention. The main structure of the low coherence interferometry part of the device is an interferometer which is composed of 11-17, 19 and 21, wherein light emitted by a light source 11 is divided into two light beams by a beam splitter 12: one beam of light enters a reference arm of the interferometer and is irradiated on a plane high reflecting mirror 14 through a reference arm collimating mirror 13; the other beam of light enters the sample arm, is focused on a sample to be measured after being collimated by 15 and reflected by a light path; the sample 18 is placed at the focal plane of the sample arm objective 17. Then the light reflected back by the reference arm and the light reflected back by the sample arm are interfered and received by the interference signal detection device 19. For the optical fiber type optical path, the polarization controller 21 is adopted to adjust the polarization state of the light beam, and the signal interference effect is maximized.
The device for treating biofilm tissue shown in fig. 2 comprises in particular, according to different modes of low coherence interference detection signals:
1) a time domain measurement device. The light source 11 uses broadband low coherent light, the plane mirror 14 can move along the optical axis direction, and the interference signal detection device 19 is a point detector. The optical path of the reference arm is changed by moving the plane mirror 14, the interference signals of the two arms are detected by the point detector 19, and the low coherence interference detection is carried out on the scattered signals in the z direction of a certain space depth, so that a sampling body of the depth space dimension is obtained.
2) Spectral domain measuring device. The light source 11 adopts broadband low-coherence light, the plane reflector 14 is fixed, and the interference signal detection device 19 adopts a spectrometer. The interference signal passes through a linear array camera in the spectrometer and simultaneously records the interference spectrum. And analyzing the interference spectrum signals by adopting a Fourier analysis method, and parallelly acquiring scattering information in the depth z direction so as to obtain a sampling body of the depth dimension space.
3) Provided is a sweep frequency measuring device. The light source 11 adopts a sweep frequency light source, the plane reflector 14 is fixed, and the interference signal detection device 19 adopts a point detector. And the point detector records the low coherence interference spectrum of the swept-frequency light source in a time-sharing manner. And (3) carrying out Fourier analysis on the interference spectrum signal, and obtaining the scattering information in the depth z direction in parallel, thereby obtaining a sampling body of the depth dimension space.
For the different measuring devices, the thickness of the biological film can be analyzed and has spatial correspondence respectively in connection with the OCT scanning imaging method referred to in the description of fig. 1.
FIG. 3 illustrates one exemplary embodiment disclosed herein that utilizes the present invention. The device for processing the biofilm tissue comprises a broadband low-coherence light source 31, an optical circulator 32, an optical fiber coupler 33 with a splitting ratio of 50:50, a first polarization controller 46, a first optical fiber collimating device 34, a focusing lens 35, a planar high-reflection mirror 36, a second polarization controller 47, a second optical fiber collimating device 37, a scanning galvanometer 38, an objective lens 39, a sample dispersing device 40, a third optical fiber collimating device 41, a grating 42, a Fourier transform lens 43, a high-speed linear array camera 44, a signal processor module and calculation unit 45, a chromatic dispersion compensator 48, a focusing lens 49, a dichroic mirror 50, a scanning lens 51, a die cutting assembly 100, and displacement tables 102 and 104 with transparent plates: vertical displacement device 104, biofilm tissue 106, transparent glass table 108, mounting bar 114, connecting arm 115, height display 118, cutting die 120. The broadband low-coherence light source 31 adopts a super-light-emitting diode light source with the central wavelength of 1325nm and the bandwidth of 100nm, the high-speed linear array camera 44 adopts a linear array scanning camera consisting of 2048 pixel units, and the scanning lens 51 in the sample arm adopts a lens with the focal length of 54 mm.
The light emitted by the low coherence broadband light source 31 used by the device of the present invention enters the optical fiber coupler 33 with a splitting ratio of 50:50 after passing through the optical circulator 32, and the light emitted from the optical fiber coupler 33 is divided into two sub-beams: one beam of light is connected to a first optical fiber collimating device 34 in the reference arm through an optical fiber through a first polarization controller 46, and is irradiated to a plane high reflecting mirror 36 after being subjected to collimation, dispersion compensation of a dispersion compensator 48 and focusing of a focusing lens 35; the other beam of light is connected to a second optical fiber collimating device 37 of the sample arm part through an optical fiber via a second polarization controller 47, and is irradiated on the measured sample after being reflected by the light path of the collimating and scanning galvanometer 38 and focused by the focusing lens 39 and the focusing lens 49. Before the scanning lens 51, the dichroic mirror 50 is used to make the 90 ° turn of the OCT probe light path by the light emitted from the focusing lens 49. The scanning galvanometer 38 in the sample arm is fixed, so that the low coherence interferometer can parallelly detect and obtain the scattering signals of the same position in the sample space in the depth direction at different moments. Meanwhile, a light path in the sample arm conducts light beams through the single-mode optical fiber, and the light scattered back by the sample to be detected plays a role in spatial filtering, namely multiple scattering components in scattering signals are effectively reduced. The light reflected by the plane high reflecting mirror 36 in the reference arm interferes with the light backscattered from the sample to be measured in the sample arm at the optical fiber coupler 33, the interference light is detected and recorded by a spectrometer (comprising devices 41-44), and then the interference light is collected by a computing unit 45 and is subjected to signal analysis processing. The vertical displacement device 104, the height display 118 and the cutting die 120 are mounted on the bar 114 by means of a connecting arm 115. The mounting bar is attached to the transparent glass tabletop 108. Sample biofilm tissue 106 is placed on a displacement stage 102 with an optically transparent plate on a transparent glass table top 108. Below the displacement stage 102, there is a transparent plate for imaging the OCT scanning beam. The translation stage 102 may effect horizontal translation. The vertical displacement device 104 is controlled by the calculation unit 45.
The thickness of the biological membrane tissue can be obtained according to the OCT depth domain signal, and the artificial heart biological valve leaflets with uniform thickness can be obtained by combining the die cutting assembly.
Fig. 4 shows the result of imaging the leaflets of the bioprosthetic heart valve after cutting, and the OCT can realize three-dimensional structural imaging for the structural image acquired by OCT. Fig. 4a is a projection view of the OCT structure, and fig. 4b is a cross-sectional view of the dashed line in fig. 4 a. Fig. 4c is a projection view of the OCT structure, and fig. 4d is a cross-sectional view of the dashed line in fig. 4 c. The thickness of any spatial location point can be obtained, and fig. 4b and 4d correspond to a uniform biofilm (thickness-0.7 mm) and a uniform biofilm (thickness-1.7 mm), respectively.
The above experimental results fully illustrate that: the invention can realize non-contact simultaneous acquisition of thickness information (micrometer precision) of a plurality of spatial position points (micrometer spacing). Meanwhile, the invention combines an optical detection system (OCT) and a die cutting component, and can realize rapid evaluation of cutting of the biological membrane tissue to obtain the leaflet of the artificial heart biological valve.
Claims (10)
1. A method for detecting and cutting a biological membrane tissue optically based on weak coherent interference comprises the following steps:
placing a die-cut assembly (100) over the biological tissue, the die-cut assembly (100) having the biofilm tissue (106) disposed therein;
detecting a thickness of a target area of the biofilm tissue (106) through the die cut assembly (100) using optical coherence imaging (OCT);
controlling a die in a die-cutting assembly (100) to cut a target area to obtain a prosthetic heart biological valve leaflet based on the thickness of the target area of the biological membrane tissue (106).
2. The optical detection cutting method for the biofilm tissue based on the weak coherent interference as claimed in claim 1, characterized in that: the biofilm tissue (106) comprising: pericardium, aortic valve, dura mater, peritoneum, septum, or intestinal submucosa.
3. The optical detection cutting method for the biofilm tissue based on the weak coherent interference as claimed in claim 1, characterized in that: detecting a thickness of the biofilm tissue (106) through the die cut assembly (100) using an optical coherence imaging system OCT; the method comprises the following steps:
acquiring an interference spectrum of the biofilm tissue (106) using OCT;
extracting a depth domain signal for an interference spectrum of the biofilm tissue (106);
a thickness measurement is made to a micron accuracy of the biofilm tissue (106) based on the depth domain signal.
4. The optical detection cutting method for the biofilm tissue based on the weak coherent interference as claimed in claim 3, characterized in that: the method for acquiring the interference spectrum of the biofilm tissue by using the OCT comprises the following steps:
a time domain OCT imaging method for changing the optical path of the reference arm by scanning;
or a spectral domain OCT imaging method for recording spectral interference signals by a spectrometer;
or a frequency-sweep OCT imaging method which utilizes a frequency-sweep light source to record spectral interference signals.
5. The optical detection cutting method for the biofilm tissue based on the weak coherent interference as claimed in claim 1, characterized in that: detecting a thickness of a biofilm tissue of a target area from below an optically transparent plate with an optical coherence imaging system (OCT), comprising: the OCT detection range is set according to the area of the target region, and the measurement sequence of different space points of the target region is determined by the OCT scanning sequence.
6. The optical detection cutting method for the biofilm tissue based on the weak coherent interference as claimed in claim 1, characterized in that: detecting a thickness of the biofilm tissue (106) through the die cut assembly (100) using an optical coherence imaging system OCT; the method comprises the following steps: during detection, the center of the detection range of the optical coherence imaging system OCT, the center of the target area of the biomembrane tissue (106) and the center of the die pattern of the cutting die are kept in the same vertical straight line.
7. The optical detection cutting method for the biofilm tissue based on the weak coherent interference as claimed in claim 1, characterized in that: cutting the target area with the mold to obtain a prosthetic heart bioprosthetic valve leaflet based on the OCT thickness measurement of the biofilm tissue, comprising:
laying biofilm tissues (106) on an optical transparent plate of a displacement table (102);
lifting a cutting die (120) over the biofilm tissue (106) by a vertical displacement device (104);
horizontally moving a displacement stage (102) until a target area of biofilm tissue (106) is located directly below the cutting die (120);
measuring a target area thickness of the biofilm tissue (106) from directly below the target area of the biofilm tissue (102) by an optical coherence imaging system OCT;
determining whether the target region thickness is suitable for acquiring a prosthetic heart valve leaflet;
and lowering the cutting mould (120) by a vertical displacement device (104) to cut the biological membrane tissue (106) of the target area so as to obtain the artificial heart biological valve leaflet.
8. The optical detection cutting method for the biofilm tissue based on the weak coherent interference as claimed in claim 1, characterized in that: the die cutting assembly (100) comprises a displacement table (102) with a transparent optical plate, a vertical displacement device (104), a transparent glass table top (108) and a cutting die (120); one side is equipped with vertical displacement device (104) on transparent glass mesa (108), and cutting die (120) set up on transparent glass mesa (108), place displacement platform (102) that have the opaque board on transparent glass mesa (108), and the tiling is placed biofilm tissue (106) on displacement platform (102).
9. The optical detection cutting method for the biofilm tissue based on the weak coherent interference as claimed in claim 8, characterized in that: the vertical displacement device (104) comprises a mounting rod (114), a connecting arm (115) and a height display (118); the bottom end of the mounting rod (114) is fixed on the transparent glass table top (108), and the height display (118) is connected to the middle part of the mounting rod (114) through a connecting arm (115); the height display (118) extends above the biological membrane tissue (106), and the cutting die (120) is fixedly arranged at the bottom end of the height display (118).
10. An optical detection cutting system for biofilm tissue based on weak coherent interference for implementing the method as claimed in any one of claims 1 to 9, comprising:
a set of die-cutting assemblies (100) for cutting the biofilm tissue (106) to obtain prosthetic heart bioprosthetic valve leaflets;
a set of OCT optical coherence detection device, which is used for collecting the interference spectrum of the biofilm tissue (106) in two-dimensional or three-dimensional space;
and one or more processors coupled to the die cutting assembly (100) and the OCT optical coherence detection device and used for respectively connecting the die cutting assembly (100) and controlling the die cutting assembly (100) to cut, receiving the OCT interference spectrum from the OCT optical coherence detection device and analyzing and processing the OCT interference spectrum to obtain the depth information of the biomembrane tissue (106).
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