CN114306432B - Plant exosome composition with anti-hair loss and hair growth functions and preparation method thereof - Google Patents
Plant exosome composition with anti-hair loss and hair growth functions and preparation method thereof Download PDFInfo
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Abstract
The invention relates to the technical field of traditional Chinese medicine compositions, and particularly discloses a plant exosome composition with a hair loss prevention and hair growth function and a preparation method thereof. The plant exosome composition comprises the following components in parts by weight: 5-15 parts of plant exosome, 10-20 parts of medicinal sage, 5-10 parts of eucommia bark, 1-8 parts of grease, 3-10 parts of skin conditioner, 8-20 parts of humectant, 1-8 parts of surfactant and 0.6-1.5 parts of thickening agent; the plant exosomes comprise a snowflake exosome, a Chinese arborvitae twig exosome and a giant knotweed exosome. The plant exosome composition can effectively promote the proliferation of the activity of hair follicle cells, balance the biological flora of the scalp, stimulate the growth of new hairs and further improve the effect of preventing alopecia and growing the hairs of the composition.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a plant exosome composition with anti-alopecia and hair-growing functions and a preparation method thereof.
Background
With the acceleration of the pace of life of modern society and the increasing deterioration of the environment, more and more people have sub-health problems of different degrees under the conditions of high-intensity mental stress, overtime, night stay, haze and the like, wherein the problems include the increased scalp grease secretion, scalp inflammation, hair follicle atrophy, hair loss and the like caused by the problems. Alopecia is a topic of increasing concern for modern people, especially young people.
The main solution to the problem of hair loss prevention and hair growth today is hair growth promoting medicine and surgery. The main drug therapy is finasteride and minoxidil, so that the side effects are serious, drug dependence is easy to form, and once the drug is stopped, alopecia is serious; the operation treatment cost is high, the wound is generated, and a certain recovery period is needed. Therefore, the development of natural anti-alopecia and hair-growing raw materials for solving the problem of alopecia has important significance.
Numerous studies have found that an extremely specific structure exists in plants: a plant exosome. The plant exosome is a nano-scale vesicle composed of phospholipid bilayers and composed of lipid, micro RNA (miRNA), protein and a large amount of active substances, the form of the plant exosome can be observed by a transmission electron microscope, and the size of the plant exosome is about 30-150 nm. The exosome derived from the medicinal plant is rich in various components with biological activity, such as lipid, protein, RNA and the like, and has obvious regulation and control effects on the aspects of skin repair, immunoregulation, anti-inflammation, anti-infection, regenerative medicine and the like. In addition, the plant exosome has the advantages of small volume, strong tissue penetrability and the like, and can maintain better physicochemical stability under different pH values and temperatures. The plant exosome has the shape and characteristics of a nano-carrier, and has remarkable advantages in the aspects of biocompatibility, stability, in-vivo distribution, half-life prolongation, cell internalization and the like. However, no relevant report is found about the application of the plant exosome in the anti-alopecia and hair-growing product.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a plant exosome composition with the functions of preventing alopecia and growing hair and a preparation method thereof. According to the invention, centella asiatica exosome, biota orientalis exosome and polygonum cuspidatum exosome are compounded, so that the prepared plant exosome composition has a remarkable anti-alopecia and hair-growing effect, and the problems that active substances or nutrient substances are difficult to absorb by scalp and the like can be solved.
In order to achieve the purpose, the invention adopts the technical scheme that:
the invention provides a plant exosome composition with a hair loss prevention and hair growth function, which comprises the following components in parts by weight:
5-15 parts of plant exosome, 10-20 parts of medicinal sage, 5-10 parts of eucommia bark, 1-8 parts of grease, 3-10 parts of skin conditioner, 8-20 parts of humectant, 1-8 parts of surfactant and 0.6-1.5 parts of thickening agent;
the plant exosomes comprise a snowflake exosome, a Chinese arborvitae twig exosome and a giant knotweed exosome.
Cacumen biotae exosomes: activating hair follicle stem cells, promoting cell activity, and promoting hair growth.
Polygonum cuspidatum exosomes: is prepared from resveratrol and emodin, and has effects of inhibiting lipid peroxide, inhibiting bacteria, promoting blood circulation, and balancing flora.
Medicinal sage: can inhibit sebum secretion.
Eucommia ulmoides: improving the content of substances such as tyrosine, vitamin C, etc., and ensuring the melanin content in melanocyte to keep hair black.
The centella asiatica exosome, the biota orientalis exosome and the polygonum cuspidatum exosome are added into the composition for compounding, so that the composition can inhibit alopecia, stimulate new hair growth, promote cell activity and accelerate microcirculation of scalp capillary blood vessels.
According to the invention, under the condition of other components in proper proportion, plant exosomes, medicinal salvia miltiorrhiza bunge and eucommia ulmoides are matched, so that the hair loss preventing and hair growing effects are better.
As a preferred embodiment of the plant exosome composition according to the present invention, the plant exosome composition comprises the following components in parts by weight:
5 to 10 parts of plant exosome, 10 to 15 parts of medicinal sage, 5 to 8 parts of eucommia bark, 1 to 5 parts of grease, 3 to 8 parts of skin conditioner, 9 to 16 parts of humectant, 1 to 5 parts of surfactant and 0.6 to 1 part of thickener.
As a preferred embodiment of the plant exosome composition, the mass ratio of the centella asiatica exosome to the cacumen biotae exosome to the giant knotweed exosome is (1-5): 2.
When the asiatic centella herb exosomes, the Chinese arborvitae twig exosomes and the giant knotweed rhizome exosomes are in the mass ratio, the proliferation of the hair follicle cell activity can be effectively promoted, and the alopecia preventing and hair growing effects of the composition are further improved.
As a preferred embodiment of the plant exosome composition, the mass ratio of the centella asiatica exosomes to the cacumen biotae exosomes to the polygonum cuspidatum exosomes is (1-3) to 1.
When the plant exosomes are preferably selected according to the mass ratio, the effect of promoting the growth of hair follicle cells is better, so that hairs are not easy to fall off, and the effect of growing the hairs can be achieved.
As a preferred embodiment of the plant exosome composition of the present invention, the skin conditioning agent is dipotassium glycyrrhizinate or/and allantoin. More preferably, the skin conditioning agent is allantoin.
As a preferred embodiment of the plant exosome composition of the present invention, the humectant is at least one of glycerin, 1, 2-hexanediol, ruga carrageenan, oat β -glucan, and sodium hyaluronate. More preferably, the humectant is a mixture of glycerol and 1, 2-hexanediol in a mass ratio of 1.
More preferably, the surfactant is a polyoxyethylated castor oil.
More preferably, the thickener is hydroxyethyl cellulose.
In addition, the present invention provides a method for preparing the above plant exosome composition, comprising the steps of:
1) Mixing the humectant, the thickener and deionized water, heating to 80-85 ℃, and uniformly stirring to obtain a material A;
2) Mixing the grease and the surfactant, heating to 75-85 ℃, and uniformly stirring to obtain a material B;
3) Adding the material A into the material B for homogenization, wherein the homogenization time is 3-7 minutes, the homogenization temperature is 75-80 ℃, after the homogenization is finished, sterilizing and cooling, vacuumizing and defoaming to obtain a mixture;
4) Adding the plant exosome, the medicinal sage, the eucommia bark and the skin conditioner into the cooled mixture, uniformly mixing, cooling to 30-35 ℃, and discharging to obtain the plant exosome composition.
As a preferred embodiment of the preparation method of the plant exosome composition of the present invention, the preparation method of the plant exosome comprises the following methods: grinding herba Centellae, folium Platycladi or rhizoma Polygoni Cuspidati, adding buffer solution, filtering, centrifuging, and collecting supernatant; and (4) carrying out ultracentrifugation on the obtained supernatant, taking the precipitate, carrying out heavy suspension on the precipitate by using a buffer solution, and taking the precipitate.
More preferably, in the preparation method of the plant exosome, the rotating speed of centrifugation is 8000 rpm-9500 rpm; the centrifugation time is 60-80 min.
The invention also provides a hair growth promoting liquid containing the plant exosome composition.
As a preferred embodiment of the hair restorer of the present invention, the plant exosome composition is present in an amount of 1 to 5wt% in the hair restorer. More preferably, the plant exosome composition is present in the hair growth tonic in an amount of 3.5wt%.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a plant exosome composition with anti-alopecia and hair-growing functions and a preparation method thereof.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to specific examples.
In the following examples and comparative examples, the experimental methods used were conventional ones unless otherwise specified, and the materials, reagents and the like used were commercially available ones unless otherwise specified.
In the following examples and comparative examples, medicinal sage, eucommia ulmoides, oils, skin conditioners, moisturizers, surfactants, and thickeners were all the same commercially available products.
Examples 1 to 10 and comparative examples 1 to 6
The formula of the plant exosome composition with the functions of preventing alopecia and growing hair comprises the following raw materials in parts by weight, and is shown in table 1.
The preparation method of the plant exosome composition comprises the following steps:
1) Mixing glycerol, 1, 2-hexanediol and hydroxyethyl cellulose according to parts by weight, heating to 82 ℃, and uniformly stirring to obtain a material A;
2) Mixing the grease and the surfactant, heating to 80 ℃, and uniformly stirring to obtain a material B;
3) Adding the material A into the material B for homogenization, wherein the homogenization time is 5 minutes, the homogenization temperature is 75 ℃, after the homogenization is finished, sterilizing and cooling, and vacuumizing and defoaming to obtain a mixture;
4) Adding the plant exosome, the medicinal sage, the eucommia bark and the skin conditioner into the cooled mixture, uniformly mixing, cooling to 35 ℃, and discharging to obtain the plant exosome composition.
The preparation method of the plant exosome comprises the following steps: grinding the roots of centella asiatica, cacumen biotae or polygonum cuspidatum by a tissue grinder, dissolving the roots of centella asiatica, cacumen biotae or polygonum cuspidatum by PBS (phosphate buffer solution) with the proportion of 1:100, filtering by gauze, centrifuging for 60min at 9000rpm, collecting supernatant, transferring the collected supernatant to an ultracentrifuge tube, accurately balancing under the condition that the error is less than 0.001g, putting the ultracentrifuge tube into a precooled ultracentrifuge, and centrifuging for 70min under the condition of 9500 Xg at the temperature of 4 ℃. The supernatant was discarded, the pellet was retained and resuspended in PBS, and after equilibration, centrifuged again at 9500 Xg for 70min at 4 ℃. After completion, the precipitate was collected, resuspended in a small amount of PBS, and then stored in an ultra-low temperature refrigerator.
TABLE 1
Adding 80 parts by weight of deionized water into the plant exosome compositions prepared in the examples 1-10 and the comparative examples 1-6 to obtain different experimental groups; taking C57BL/6 pure-breed mice with the weight of 15-20g, using both male and female, under the light anesthesia of ether, pulling out the hair on the back of the mice by using a mixture of rosin and paraffin, and dividing the mice into groups with the area of about 3cm multiplied by 4cm, namely a normal control group and each test group. And (3) uniformly coating the test group standby products on the skin of the hair removal area on the back of each group of mice respectively on the next day after hair removal, wherein the dosage of each test group is 10ml/kg, the control group is provided with a normal saline group with the same volume for 1 time/day for 10 days continuously, the skin of the hair removal area on the back of the mice is taken 10 days after the application of the drugs, fixed by formalin, made into pathological sections, and the growth condition of hair follicles is observed.
The results are shown in Table 2.
TABLE 2
Note: in comparison with the control group, ** P〈0.01， * P〈0.05。
according to the results of table 2, the plant exosome compositions prepared in examples 1 to 10 can promote the increase of hair follicle number, balance the biological flora of scalp, stimulate new hair growth, and have a better effect on hair growth, relative to the control group.
Comparative example 1 lacks asiatic pennywort herb exosomes, has insignificant hair growth and growth promoting effects, and cannot significantly increase the number of hair follicles; comparative examples 3 and 4 lack the cacumen biotae exosomes or the polygonum cuspidatum exosomes, and the number of hair follicles is greater than that of comparative example 1, which shows that the centella asiatica exosomes have a greater influence on the increase of the number of hair follicles than the cacumen biotae exosomes or the polygonum cuspidatum exosomes; in the absence of either one of the cacumen biotae exosomes and the polygonum cuspidatum exosomes, the hair growth and hair follicle number promoting effect is certain, but the effect is still inferior to that of example 3.
Comparative examples 2 and 5, the weight parts of asiatic centella exosomes are increased, or the mass ratio of asiatic centella exosomes to biota exosomes to giant knotweed exosomes is changed, the number of hair follicles is less than that of example 3, and the fact that the weight parts of asiatic centella exosomes are within the range of the invention is shown to promote the growth of the hair follicles, increase the number of hair follicles and help the hair growth. The mass ratio of the asiatic centella exosomes to the cacumen biotae exosomes to the giant knotweed exosomes is not (1-5): 2, the growth of hair follicles can be influenced, and alopecia prevention and hair growth are not facilitated.
Comparative example 6 shows that the amount of eucommia ulmoides added by weight is less than that of example 3, and the effect of eucommia ulmoides added by weight to promote the growth of hair follicles is less than that of example 3.
Test examples
The plant exosome compositions prepared in examples 1 to 10 and comparative examples 1 to 6 were formulated into a hair-restorer solution with a content of 3.5wt%.
1) 50 patients are selected, wherein male accounts for 80 percent, female accounts for 20 percent, the longest course of disease is 10 years, the shortest course of disease is 2 years, and the age is between 20 and 60 years.
2) The prepared hair growth promoting liquid is used as a test medicine, a patient uses the test medicine to externally coat or spray the test medicine on an affected part, the dosage is 2ml each time, 2 times (once in the morning and at night) each day, the administration is 4 weeks as a treatment course, the treatment course is 4 treatment courses, and the middle is uninterrupted.
3) And (3) determination standard:
the effect is shown: no alopecia phenomenon, and growing exuberant hair on the part with baldness and becoming thick and black gradually;
the method has the following advantages: no alopecia phenomenon, and sparse hair grows at the position where the scalp is bald and sparse;
and (4) invalidation: has alopecia phenomenon, and no hair grows at bald part of scalp. The results of clinical treatment of 50 patients are shown in table 3.
TABLE 3
| Group of | Show effect | Is effective | Invalidation | High efficiency |
| Example 1 | 47 | 3 | 0 | 100％ |
| Example 2 | 48 | 2 | 0 | 100％ |
| Example 3 | 49 | 1 | 0 | 100％ |
| Example 4 | 45 | 5 | 0 | 100％ |
| Example 5 | 45 | 5 | 0 | 100％ |
| Example 6 | 46 | 4 | 0 | 100％ |
| Example 7 | 43 | 6 | 1 | 98％ |
| Example 8 | 47 | 3 | 0 | 100％ |
| Example 9 | 48 | 2 | 0 | 100％ |
| Example 10 | 47 | 3 | 0 | 100％ |
| Comparative example 1 | 8 | 12 | 30 | 40％ |
| Comparative example 2 | 33 | 3 | 14 | 72％ |
| Comparative example 3 | 36 | 3 | 11 | 78％ |
| Comparative example 4 | 38 | 4 | 8 | 84％ |
| Comparative example 5 | 35 | 5 | 10 | 80％ |
| Comparative example 6 | 40 | 8 | 2 | 86％ |
As can be seen from Table 3, the hair growth liquid prepared from the plant exosome compositions of examples 1-10 has an effective rate of more than or equal to 98% for patients, which indicates that the plant exosome composition can nourish hair follicles, prevent hair loss and grow hair, and has good comprehensive effect.
The comparative example 1 does not contain asiatic pennywort herb exosomes, the treatment effective rate is only 40%, and the fact that the asiatic pennywort herb exosomes with certain content are added into the composition can improve the hair growth effect; comparative examples 3-4 lack cacumen biotae exosomes or polygonum cuspidatum exosomes, the effective rate is lower than that of example 3, and the results show that the centella asiatica exosomes, the cacumen biotae exosomes and the polygonum cuspidatum exosomes and other active components added into the composition can act synergistically, so that the hair follicle cell activity proliferation can be effectively promoted, the scalp biological flora is balanced, new hair growth is stimulated, and the hair loss prevention and hair growth effects of the composition are further improved.
Comparative examples 2 and 5 change the mass ratio of asiatic centella exosomes, biota orientalis exosomes and giant knotweed exosomes, and the treatment effective rate is not as high as that of the composition prepared by the mass ratio of asiatic centella exosomes, biota orientalis exosomes and giant knotweed exosomes (1-5): 2.
Comparative example 6 the effective rate of treatment is reduced by increasing the parts by weight of eucommia ulmoides, which indicates that the effective rate of treatment is affected by the parts by weight of eucommia ulmoides.
On the basis of adding centella asiatica exosome, biota orientalis exosome and polygonum cuspidatum exosome, other components and a proper proportion are combined, so that the plant exosome composition prepared by the invention can improve the treatment effective rate of patients with alopecia, and the comprehensive effect is better.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (9)
1. The plant exosome composition with the functions of preventing alopecia and growing hair is characterized by comprising the following components in parts by weight:
5 to 15 parts of plant exosome, 10 to 20 parts of medicinal sage, 5 to 10 parts of eucommia bark, 1 to 8 parts of grease, 3 to 10 parts of skin conditioner, 8 to 20 parts of humectant, 1 to 8 parts of surfactant and 0.6 to 1.5 parts of thickener;
the plant exosomes are snowplant exosomes, chinese arborvitae twig exosomes and giant knotweed exosomes;
the mass ratio of the asiatic centella exosome to the cacumen biotae exosome to the giant knotweed exosome is (1-5) to 1.
2. The plant exosome composition according to claim 1, consisting of the following components in parts by weight:
5 to 10 parts of plant exosome, 10 to 15 parts of medicinal sage, 5 to 8 parts of eucommia bark, 1 to 5 parts of grease, 3 to 8 parts of skin conditioner, 8 to 16 parts of humectant, 1 to 5 parts of surfactant and 0.6 to 1 part of thickener.
3. The plant exosome composition according to claim 1, wherein the mass ratio of centella asiatica exosomes, biota orientalis exosomes and polygonum cuspidatum exosomes is (1-3) to 2.
4. A plant exosome composition according to claim 1, wherein the skin conditioning agent is dipotassium glycyrrhizinate or/and allantoin.
5. The plant exosome composition of claim 1, wherein said humectant is at least one of glycerol, 1, 2-hexanediol, carrageen crispa, oat beta-glucan and sodium hyaluronate.
6. A method of preparing a plant exosome composition according to any one of claims 1-5, comprising the steps of:
1) Mixing the humectant and the thickener, heating to 80-85 ℃, and uniformly stirring to obtain a material A;
2) Mixing the grease and the surfactant, heating to 75-85 ℃, and uniformly stirring to obtain a material B;
3) Adding the material A into the material B for homogenization, wherein the homogenization time is 3-7 minutes, the homogenization temperature is 75-80 ℃, after the homogenization is finished, sterilizing and cooling, and vacuumizing and defoaming to obtain a mixture;
4) Adding the plant exosome, the medicinal sage, the eucommia bark and the skin conditioner into the cooled mixture, uniformly mixing, cooling to 30-35 ℃, and discharging to obtain the plant exosome composition.
7. The method of claim 6, wherein the method of preparing the plant exosomes comprises the following steps: grinding herba Centellae, folium Platycladi or rhizoma Polygoni Cuspidati, adding buffer solution, filtering, centrifuging, and collecting supernatant; and (4) carrying out ultracentrifugation on the obtained supernatant, taking the precipitate, carrying out heavy suspension on the precipitate by using a buffer solution, and taking the precipitate.
8. A hair growth tonic comprising the plant exosome composition according to any one of claims 1 to 5.
9. The hair growth lotion according to claim 8, wherein the content of the plant exosome composition in the hair growth lotion is 1 to 5wt%.
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| CN116036196A (en) * | 2022-12-29 | 2023-05-02 | 广州远想生物科技股份有限公司 | Anti-hair loss hair-nourishing essence and preparation method thereof |
| CN116942847B (en) * | 2023-08-30 | 2024-04-09 | 深圳市华健生物技术有限公司 | Drug-loaded plant exosome targeting hair follicle, and preparation method and application thereof |
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