CN114306416B - Astragalus polysaccharide complex and its application in antibiosis and immunity enhancement - Google Patents
Astragalus polysaccharide complex and its application in antibiosis and immunity enhancement Download PDFInfo
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses an astragalus polysaccharide compound and application thereof in antibiosis and immunity enhancement, wherein the astragalus polysaccharide compound is composed of the following raw materials in parts by weight: 8-12 parts of astragalus polysaccharide, 4-6 parts of propyl gallate and 4-6 parts of rhodiola flavone. The invention takes astragalus polysaccharide as a base, and inspects the improvement effect of the compound astragalus polysaccharide on the immunity and the antibacterial ability by compounding the existing substances with the antibacterial and the immunity-enhancing capability with the astragalus polysaccharide. The result shows that after the astragalus polysaccharide, the propyl gallate and the rhodiola rosea flavone are compounded, the astragalus polysaccharide, the propyl gallate and the rhodiola rosea flavone have a synergistic effect on the aspects of improving the immunity and the antibacterial ability. Is expected to be developed into a pharmaceutical preparation or a feed additive with antibacterial and immunity enhancing functions, and has wide application prospect in the aspect of large-scale animal breeding.
Description
Technical Field
The invention belongs to the technical field of medicines, and particularly relates to an astragalus polysaccharide compound and application thereof in antibiosis and immunity enhancement.
Background
With the scale and intensive development of the breeding industry, the management concept of 'prevention over treatment' is accepted by people in the industry. In the prevention of animal diseases, the improvement of immune function and antibacterial function is the basis and key of animal disease resistance.
Radix Astragali (Radix Astragali) is a traditional Chinese medicine for tonifying in China, and modern pharmacological studies prove that Radix Astragali has the function of enhancing the immunity of organisms, the main active ingredients of the Radix Astragali comprise polysaccharide and saponin, and the active ingredients can improve the disease resistance of animals and reduce the morbidity. Wherein, the astragalus polysaccharide is the substance with the strongest immune activity and consists of water-soluble heteropolysaccharide such as glucose, galactose, fructose, arabinose and the like. The astragalus polysaccharide has biological activities of immunoregulation, antioxidation, antitumor, antidiabetic, cardiovascular system protection, anti-inflammation, animal growth promotion and the like, does not have the problem of drug residue, and can be used as a feed additive and an immunopotentiator.
At present, astragalus polysaccharide is mainly used as an immunopotentiator in veterinary drugs, but few research reports on bacteriostasis are available. Generally, the bacteriostatic action of astragalus polysaccharide is not significant. If the prevention capability of animals to diseases is improved by improving the immune function and the antibacterial function, the disease prevention requirement is difficult to meet by singly adopting the astragalus polysaccharide. Therefore, it is one of the hot directions of research to improve the antibacterial and immune functions by compounding astragalus polysaccharides with other substances.
Propyl Gallate (PG) is one of food antioxidants approved for use by FAO/WHO tissues and belongs to natural food antioxidants. At present, the method has wide application in the fields of food, fruit and vegetable fresh keeping, medicine, cosmetics, feed, photosensitive and thermosensitive materials and the like. Radix Rhodiolae flavone has effects of resisting tumor, DNA distortion, aging, fatigue, anoxia, radiation, and sleep. However, the report of the compound application of astragalus polysaccharide, propyl gallate and rhodiola rosea flavone in the aspects of antibiosis and immunity enhancement is not found at present.
Disclosure of Invention
Aiming at the prior art, the invention aims to provide an astragalus polysaccharide compound and application thereof in antibiosis and immunity enhancement.
In order to realize the purpose, the invention adopts the following technical scheme:
the invention provides an astragalus polysaccharide compound, which consists of the following raw materials in parts by weight:
8-12 parts of astragalus polysaccharide, 4-6 parts of propyl gallate and 4-6 parts of rhodiola flavone.
Preferably, the astragalus polysaccharide compound is prepared from the following raw materials in parts by weight:
10 parts of astragalus polysaccharide, 5 parts of propyl gallate and 5 parts of rhodiola rosea flavone.
In a second aspect of the present invention, a method for preparing the astragalus polysaccharide complex is provided, which comprises the following steps:
mixing radix astragali polysaccharide, propyl gallate and radix Rhodiolae flavone in parts by weight.
In a third aspect of the present invention, there is provided an application of the above astragalus polysaccharide complex in the preparation of a pharmaceutical preparation having antibacterial and immune enhancing functions simultaneously.
The fourth aspect of the invention provides an application of the astragalus polysaccharide compound in preparing a feed additive.
In a fifth aspect of the present invention, a feed additive is provided, wherein the feed additive comprises the above astragalus polysaccharide complex as an active ingredient.
Further, the feed additive may also comprise carriers and/or auxiliary materials, such as: corn flour, wheat bran, defatted rice bran, calcium carbonate, silicate, vermiculite and the like.
Preferably, the feed additive can be prepared into powder, granules, pellets and the like.
The invention has the beneficial effects that:
because the improvement of the antibacterial effect by adopting single astragalus polysaccharide is limited, under the management concept of prevention and treatment, aiming at improving the immune function and the antibacterial ability of animals, the invention takes the astragalus polysaccharide as the basis, and inspects the improvement effect of the immunity and the antibacterial ability after the astragalus polysaccharide is compounded by compounding the existing substances with antibacterial and immunity-enhancing capabilities with the astragalus polysaccharide. The result shows that after the astragalus polysaccharide, the propyl gallate and the rhodiola rosea flavone are compounded, the astragalus polysaccharide, the propyl gallate and the rhodiola rosea flavone have a synergistic effect on the aspects of improving the immunity and the antibacterial ability. Is expected to be developed into a pharmaceutical preparation or a feed additive with antibacterial and immunity enhancing functions, and has wide application prospect in the aspect of large-scale animal breeding.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
In order to make the technical solutions of the present application more clearly understood by those skilled in the art, the technical solutions of the present application will be described in detail below with reference to specific embodiments.
The test materials used in the examples of the present invention are all conventional in the art and commercially available. Wherein:
astragalus polysaccharides were purchased from Heng Rui Tong Dai Biotech, inc., sichuan under product batch No. 180601.
Propyl gallate was purchased from Hunan galloya Biotechnology Ltd, product batch number 201809017.
Rhodiola flavone was purchased from Hengreton bioscience, sichuan under product batch No. 180904.
Example 1: preparation of astragalus polysaccharide complex
1. The raw materials comprise:
10g of astragalus polysaccharide, 5g of propyl gallate and 5g of rhodiola rosea flavone.
2. The preparation method comprises the following steps:
weighing the astragalus polysaccharide, the propyl gallate and the rhodiola rosea flavone according to the raw material composition, and uniformly mixing the materials to obtain the astragalus polysaccharide compound.
Example 2: preparation of astragalus polysaccharide complex
1. The raw materials comprise:
12g of astragalus polysaccharide, 4g of propyl gallate and 6g of rhodiola rosea flavone.
2. The preparation method comprises the following steps: the same as in example 1.
Example 3: preparation of astragalus polysaccharide complex
1. The raw materials comprise:
8g of astragalus polysaccharide, 6g of propyl gallate and 4g of rhodiola rosea flavone.
2. The preparation method comprises the following steps: the same as in example 1.
Test example 1: investigation and research on influence of astragalus polysaccharide compound on immune function
1. Cell assay
Lymphocytes, when cultured in vitro, are stimulated by various factors and can exhibit increased cell volume, vigorous metabolism, increased synthesis of proteins and nucleic acids, i.e., transformation into and proliferation of lymphoblasts, a phenomenon known as lymphocyte transformation. The proliferation level of the lymphocytes is an important index for representing the immune response level of T lymphocytes and B lymphocytes.
(1) The test method comprises the following steps:
the chicken peripheral blood T lymphocyte proliferation test is carried out according to the 'purification and identification of platycodon grandiflorum polysaccharide and immunoregulation activity of platycodon grandiflorum polysaccharide on chicken peripheral blood lymphocytes' (master academic paper of Shandong university of agriculture, 2016), and specifically comprises the following steps:
counting the peripheral blood lymphocytes of the chicken, adjusting the cell concentration to 1.5 multiplied by 10 by RPMI1640 culture solution after the viable cells are more than 90 percent 6 each.mL -1 The other half of the total amount was added with Phytohemagglutinin (PHA) at a final concentration of 10. Mu.g.mL -1 Half of the cells were added to 96-well cell culture plates without PHA, 80. Mu.L -1 Then 20. Mu.L of RPMI1640 was added to make up to 100. Mu.L.
The experiment was set up in 4 experimental groups, in which:
the astragalus polysaccharide complex prepared in example 1 was added to each well of test group 1 at a concentration of 50 μ g/ml;
the astragalus polysaccharide with the concentration of 50 mug/ml is added into each hole of the test group 2;
propyl gallate was added to each well of trial group 3 at a concentration of 50 μ g/ml;
rhodiola rosea flavone with the concentration of 50 mug/ml is added into each hole of the test group 4;
repeat 4 wells per sample, with Cell Control (CC) and PHA Control (PC) added, cell control without anything added; phytohemagglutinin (PHA) control group was added at 10. Mu.g/mL -1 The other treatments were the same as the test groups. 38.5 deg.C, 5% CO 2 Culturing under the condition for 44h, taking out, adding 30 μ L MTT into each well, culturing for 4h, adding 100 μ L lysate DMSO into each well, placing the cell plate on a micro-oscillator, shaking for 5min to dissolve the precipitate completely, and detecting the light absorption value (A) at 570nm on an enzyme-linked immunosorbent assay 570 Value) as an index of T lymphocyte proliferation. When test group A 570 Values significantly greater than the control group indicate that the test drug significantly promoted T lymphocyte proliferation (Wang et al,2005 guo et al, 2009. And calculating the highest lymphocyte proliferation rate of each test group according to the formula: maximum lymphocyte proliferation rate = (maximum a in test group) 570 Value-cell control group A 570 Value)/cell control group A 570 Value X100%.
(2) And (3) test results:
the results of the measurement of the highest lymphocyte proliferation rate of the 4 test groups are shown in Table 1.
Table 1: measurement of maximum lymphocyte proliferation Rate
Group of | Highest lymphocyte proliferation rate (%) |
Test group 1 | 85.4 |
Test group 2 | 32.0 |
Test group 3 | 12.5 |
Test group 4 | 20.6 |
The results show that: the astragalus polysaccharide, the propyl gallate and the rhodiola rosea flavone are used in a combined mode, and the proliferation of peripheral blood lymphocytes of the chicken can be synergistically promoted.
2. Animal experiments:
(1) The test method comprises the following steps:
the experiment is carried out by referring to a method of 'exploring the immunity function of a normal mouse regulated by astragalus and compound combination thereof' (Chinese modern Chinese medicine, 20 th volume, 5 th stage, 5 months in 2018), and the method comprises the following specific steps:
BALB/c mice, male, 18-22 g, after adaptive feeding for 3d, randomly divided into 5 groups, each group containing 10 mice, which are blank group, astragalus polysaccharide complex group, astragalus polysaccharide group, propyl gallate group and rhodiola rosea flavone group; wherein:
astragalus polysaccharide complex group the astragalus polysaccharide complex prepared in example 1 was intragastric;
intragastric astragalus polysaccharides;
the propyl gallate group is intragastric propyl gallate;
rhodiola rosea flavone group perfusing rhodiola rosea flavone;
the dosage of each group is 0.1g/kg of mouse body mass, and the blank group is given the same amount of distilled water; the administration is carried out 1 time per day for 14 days. All groups had normal diet and drinking water.
After the last gastric lavage, the mice are fasted for 12 hours, the body mass of the mice is measured, the mice are killed after the neck is removed, the spleen and the thymus are picked up to be weighed, and the organ index is calculated according to the following formula.
Organ index (mg. G) -1 ) = mass of organ (mg)/body mass (g)
(2) And (3) test results:
the measurement results of organ indexes of each group are shown in Table 2.
Table 2: index of mouse organs
Group of | Spleen index (mg. G) -1 ) | Thymus index (mg. G) -1 ) |
Blank group | 3.12±0.15 | 1.28±0.10 |
Astragalus polysaccharide complex group | 4.05±0.20 | 1.84±0.15 |
Astragalus polysaccharides group | 3.46±0.18 | 1.40±0.12 |
Propyl gallate group | 3.28±0.13 | 1.35±0.10 |
Rhodiola flavone group | 3.20±0.12 | 1.32±0.10 |
The spleen index and thymus index were able to visually reflect the immune function of the animals, as can be seen from table 2: the astragalus polysaccharide, the propyl gallate and the rhodiola rosea flavone are compounded, so that the immune function of a mouse can be obviously improved.
Test example 2: investigation and research on influence of astragalus polysaccharide compound on antibacterial function
1. The test method comprises the following steps:
preparing the astragalus polysaccharide complex, the astragalus polysaccharide, the propyl gallate and the rhodiola flavone prepared in the example 1 into medicinal stock solutions with the concentration of 1200 mu g/ml respectively; the bacteria, the culture medium and the drugs are inoculated by a 96-well dot matrix culture plate, the bacteria culture is carried out, and the Minimum Inhibitory Concentration (MIC) of the drugs is observed.
Specific methods of inoculation, culture conditions, and interpretation of the end points were referred to "veterinary pathogen agar dilution drug susceptibility test Specification" (DB 37/T2806-2016).
2. And (3) test results:
quality control strains of astragalus polysaccharide complex, astragalus polysaccharide, propyl gallate and rhodiola rosea flavone prepared in example 1Andthe results of MIC value determination are shown in Table 3.
Table 3: results of antibacterial tests
The above results show that: the astragalus polysaccharide, the propyl gallate and the rhodiola rosea flavone are compounded, so that the quality control strain can be obviously improvedAndthe antibacterial activity of (1).
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims (2)
1. The application of the astragalus polysaccharide compound in preparing a medicinal preparation with antibacterial and immunity enhancing functions;
the astragalus polysaccharide compound is prepared from the following raw materials in parts by weight:
8-12 parts of astragalus polysaccharide, 4-6 parts of propyl gallate and 4-6 parts of rhodiola flavone.
2. The use of claim 1, wherein the astragalus polysaccharide complex is prepared from the following raw materials in parts by weight:
10 parts of astragalus polysaccharide, 5 parts of propyl gallate and 5 parts of rhodiola rosea flavone.
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CN1849927A (en) * | 2006-05-24 | 2006-10-25 | 张彦秋 | Health-care product for relieving fatigue and intensifying immunity and preparing method |
CN101433631A (en) * | 2007-11-16 | 2009-05-20 | 北京因科瑞斯医药科技有限公司 | Antiradiation composition for improving immunity and preparation method thereof |
CN107467666A (en) * | 2017-09-19 | 2017-12-15 | 房洪全 | It is a kind of to improve health food of immunity and preparation method thereof |
CN113274503A (en) * | 2021-06-08 | 2021-08-20 | 山东鑫谷健康产业有限公司 | Plant polyphenol substitute antibiotic additive and application thereof |
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CN1849927A (en) * | 2006-05-24 | 2006-10-25 | 张彦秋 | Health-care product for relieving fatigue and intensifying immunity and preparing method |
CN101433631A (en) * | 2007-11-16 | 2009-05-20 | 北京因科瑞斯医药科技有限公司 | Antiradiation composition for improving immunity and preparation method thereof |
CN107467666A (en) * | 2017-09-19 | 2017-12-15 | 房洪全 | It is a kind of to improve health food of immunity and preparation method thereof |
CN113274503A (en) * | 2021-06-08 | 2021-08-20 | 山东鑫谷健康产业有限公司 | Plant polyphenol substitute antibiotic additive and application thereof |
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