CN114304188A - Verticillium lecanii dispersible oil suspending agent and preparation method and application thereof - Google Patents
Verticillium lecanii dispersible oil suspending agent and preparation method and application thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of pesticide preparations, and relates to a verticillium lecanii dispersible oil suspending agent and a preparation method thereof. The invention provides a verticillium lecanii dispersible oil suspending agent, which comprises verticillium lecanii spore powder, a dispersion medium, an emulsifier, a dispersant, a viscosity regulator and an ultraviolet protective agent, wherein the dispersion medium is one of soybean oil and methyl oleate; the emulsifier is one of the combination of vegetable oil emulsifier ZR-5 and soybean oil emulsifier, and the combination of OP-10 and span 80; the dispersant is one of calcium lignosulfonate and Tween-80; the viscosity regulator is organic bentonite; the ultraviolet protective agent is fluorescein sodium. The verticillium lecanii dispersible oil suspending agent provided by the invention fully utilizes the living microorganism preparation to obtain the verticillium lecanii preparation with dispersible oil suspending agent performance and good insecticidal effect.
Description
Technical Field
The invention belongs to the technical field of pesticide preparations, and relates to a verticillium lecanii dispersible oil suspending agent.
Background
The pesticide formulation is developing towards multifunction, refinement, labor saving and slow release, and some new formulations which are safe, efficient, economical and environment-compatible are continuously replacing missible oil, powder and other formulations with great pollution. The suspending agent not only avoids the pollution of the powder to the environment, but also avoids the poison of the benzene solvent in the missible oil to mammals. Dispersible Oil suspension (OD) is a new formulation, which is a liquid preparation with active ingredients stably suspended in water-immiscible liquid, and is used after being directly diluted with water. The dispersible oil suspending agent has the advantages of small particle size, easy permeation, no bad smell, high suspension rate and the like, can be mixed with an auxiliary agent for use, can obviously increase the deposition amount of the agent on the surface of a target, is resistant to rain wash, and is convenient for mechanical application.
The verticillium lecanii (Lecanicillium lecanii) is an entomopathogenic fungus, is used as a living biological pesticide, has a completely new action mechanism different from the existing chemical pesticide, has no pollution to the environment and no residue, can be used as a substitute control drug for controlling certain insects which have drug resistance to the bacillus thuringiensis, and has important economic significance for developing and utilizing the verticillium lecanii. The verticillium lecanii is wide in distribution, has good control effects on bemisia tabaci, trialeurodes vaporariorum, aphids, thrips and the like, and has wide application prospects. At present, the market mainly takes beauveria bassiana and metarhizium anisopliae preparations as main materials, but the quantity of commercial preparations of verticillium lecanii is small, so that the application of verticillium lecanii needs to be researched urgently.
Conidia of verticillium lecanii are easily influenced by temperature and humidity, and the storage period is short under the condition of room temperature, but the conidia can prolong the storage period and reduce the influence of temperature and humidity when being stored in an oil carrier. The dispersing medium of the dispersible oil suspending agent comprises vegetable oil, mineral oil, higher aliphatic hydrocarbon, polyalcohol and the like, wherein the vegetable oil and the methylated vegetable oil are from plants, the degradability in the environment is better, the using amount of the dispersing medium is gradually increased, the dispersing medium has the tendency of replacing mineral oil as the dispersing medium, the advantages of taking the dispersible oil suspending agent which takes the vegetable oil and the derivatives thereof as the dispersing medium as an environment-friendly preparation are fully reflected, and the dispersing medium has popularization and application values in practical application. The research on the verticillium lecanii dispersible oil suspending agent has important significance for pest control, pesticide residue reduction, environment protection and income increase promotion. At present, the preparation of verticillium lecanii is few at home, but the dispersible oil suspending agent is almost not available. Therefore, the development of the verticillium lecanii dispersible oil suspending agent with high storage stability and good performance has important significance.
Disclosure of Invention
The invention aims to develop the verticillium lecanii dispersible oil suspending agent with high storage stability and good performance.
In view of the above, the present invention addresses this need in the art by providing a dispersible oil suspension of verticillium lecanii.
On one hand, the invention relates to a verticillium lecanii dispersible oil suspending agent, which comprises verticillium lecanii spore powder, a dispersing medium, an emulsifier, a dispersant, a viscosity regulator and an ultraviolet protective agent; the weight ratio of the verticillium lecanii spore powder to the dispersion medium to the emulsifier to the dispersant to the viscosity regulator to the ultraviolet protective agent is 5:70:20:1:3: 1.
Further, in the verticillium lecanii dispersible oil suspending agent provided by the invention, a dispersion medium is one of soybean oil and methyl oleate, an emulsifier is one of a combination of a vegetable oil emulsifier ZR-5 and a soybean oil emulsifier and a combination of OP-10 and span 80, a dispersant is one of calcium lignosulfonate and Tween-80, a viscosity regulator is organic bentonite, and an ultraviolet protective agent is sodium fluorescein.
Further, in the verticillium lecanii dispersible oil suspending agent provided by the invention, a dispersion medium is soybean oil, an emulsifier is a combination of a vegetable oil emulsifier ZR-5 and a soybean oil emulsifier, a dispersant is calcium lignosulfonate, a viscosity regulator is organic bentonite, and an ultraviolet protective agent is sodium fluorescein; the ratio of the vegetable oil emulsifier ZR-5 to the soybean oil emulsifier is 3: 2-4: 1 by mass ratio.
Furthermore, in the verticillium lecanii dispersible oil suspending agent provided by the invention, a dispersion medium is methyl oleate, an emulsifier is a combination of OP-10 and span-80, a dispersant is Tween-80, a viscosity regulator is organic bentonite, and an ultraviolet protective agent is fluorescein sodium; the mass ratio of the OP-10 to the span 80 is 1: 1-3: 2.
On the other hand, the invention provides application of the verticillium lecanii dispersible oil suspension agent in the aspect of insect killing, particularly in the aspect of killing peach aphids.
On the other hand, the invention provides a preparation method of verticillium lecanii spore powder, which comprises the following steps: transferring Verticillium lecanii strain (L.lecanii patent preservation number CCTCC NO: M218303) stored in PDA slant at 4 deg.C into PDA plate medium, culturing at (25 + -1) deg.C for 7 days, eluting spores with sterile water, and making into 1 × 107cfu/mL spore suspension, stored at 4 ℃ until use. Soaking rice in distilled water for 30min, draining, subpackaging in 1L tissue culture bottles with each bottle being 300mL, sterilizing at 121 deg.C for 30min, cooling to room temperature, inoculating spore suspension at 1%, wherein the inoculated spore suspension at 1% is prepared by inoculating 1mL of spore suspension into 100mL of culture medium. After the rice in the tissue culture bottle is uniformly dispersed by fully shaking, the rice is cultured for 12D in an incubator with the L: D being 12:12 at the temperature of 25 +/-2 ℃. Sieving the rice after 12d culture by a standard sieve of 200 meshes to obtain spore powder, namely the Verticillium lecanii spore powder.
In another aspect, the invention provides a preparation method of a verticillium lecanii dispersible oil suspending agent, which comprises the following steps: weighing 70 parts of dispersion medium, 20 parts of emulsifier, 1 part of dispersant, 3 parts of viscosity regulator and 1 part of ultraviolet protective agent, mixing and shearing for 30min, and then adding 5 parts of verticillium lecanii spore powder under the ice bath condition and uniformly shearing to obtain the verticillium lecanii dispersible oil suspending agent.
Further, in the preparation method of the verticillium lecanii dispersible oil suspending agent provided by the invention, the dispersion medium is soybean oil, the emulsifier is a combination of vegetable oil emulsifier ZR-5 and soybean oil emulsifier, the dispersant is calcium lignosulfonate, the viscosity regulator is organic bentonite, and the ultraviolet protective agent is sodium fluorescein; the ratio of the vegetable oil emulsifier ZR-5 to the soybean oil emulsifier is 3: 2-4: 1 by mass ratio.
Furthermore, in the preparation method of the verticillium lecanii dispersible oil suspending agent provided by the invention, the dispersing medium is methyl oleate, the emulsifier is the combination of OP-10 and span-80, the dispersant is Tween-80, the viscosity regulator is organic bentonite, and the ultraviolet protective agent is fluorescein sodium; the mass ratio of the OP-10 to the span 80 is 1: 1-3: 2.
Compared with the prior art, the invention has the following beneficial effects or advantages:
compared with the prior art, the main contribution of the invention is to provide the verticillium lecanii dispersible oil suspending agent. The inventor of the patent tests the performance of the verticillium lecanii strain, and finds that the verticillium lecanii strain has outstanding performance, low infectious microbe rate, high suspension rate, weak pH value, good pourability and good wet sieve test performance, greatly improves the storage stability of the verticillium lecanii, and realizes the retention of the virulence effect of the verticillium lecanii.
Drawings
FIG. 1 is a 3D response surface and overlay contour plot of oil drainage.
FIG. 2 is a graph showing the storage stability of a Verticillium lecanii dispersible oil suspension.
FIG. 3 is a diagram showing the infection effect of verticillium lecanii dispersible oil suspending agent on myzus persicae; fig. 3-A shows 24h after the semi-finished product is taken as a medicine, and fig. 3-B shows 24h after the verticillium lecanii can disperse the oil suspending agent.
Detailed Description
The following examples are given to illustrate the technical aspects of the present invention, but the present invention is not limited to the following examples.
Example 1
This example provides the preparation of verticillium lecanii spore powder in a verticillium lecanii dispersible oil suspension.
(1) Transferring Verticillium lecanii strain (L.lecanii patent preservation number CCTCC NO: M218303) stored in PDA slant at 4 deg.C into PDA plate medium, culturing at (25 + -1) deg.C for 7 days, eluting spores with sterile water, and making into 1 × 107cfu/mL spore suspension, stored at 4 ℃ until use.
(2) Soaking rice in distilled water for 30min, draining, subpackaging in 1L tissue culture bottles with each bottle being 300mL, sterilizing at 121 deg.C for 30min, cooling to room temperature, inoculating spore suspension at 1% and inoculating 1mL of (1) spore suspension in 100mL of culture medium. After the rice in the tissue culture bottle is uniformly dispersed by fully shaking, the rice is cultured for 12D in an incubator with the L: D being 12:12 at the temperature of 25 +/-2 ℃.
(3) Sieving the rice after 12 days of culture through a 200-mesh standard sieve to obtain spore powder, and storing at 4 ℃. The spore content is about 4.87X 109Per g, water content 11%.
Example 2
This example provides a dispersible oil suspension of Verticillium lecanii with soybean oil as the dispersion medium.
Verticillium lecanii spore powder was obtained in example 1.
The components are as follows: 5 parts of verticillium lecanii spore powder, 70 parts of soybean oil, 70 parts of vegetable oil emulsifier ZR-516 parts, 4 parts of soybean oil emulsifier, 1 part of calcium lignosulphonate, 3 parts of organic bentonite and 1 part of sodium fluorescein.
Weighing quantitative soybean oil, a vegetable oil emulsifier ZR-5, a soybean oil emulsifier, calcium lignosulfonate, organic bentonite and sodium fluorescein according to the mixture ratio requirement, mixing and shearing for 30min, and then adding verticillium lecanii spore powder under the ice bath condition and shearing uniformly to obtain the verticillium lecanii dispersible oil suspending agent.
Example 3
This example provides a dispersible oil suspension of Verticillium lecanii with methyl oleate as the dispersing medium.
Verticillium lecanii spore powder was obtained in example 1.
The components are as follows: 5 parts of verticillium lecanii spore powder, 70 parts of methyl oleate, OP-1012 parts, 808 parts of span, 801 parts of Tween-801 parts, 3 parts of organic bentonite and 1 part of fluorescein sodium.
Weighing quantitative methyl oleate, OP-10, span 80, Tween-80, organic bentonite and sodium fluorescein according to the mixture ratio requirement, mixing and shearing for 30min, and then adding verticillium lecanii spore powder under the ice bath condition and uniformly shearing to obtain the verticillium lecanii dispersible oil suspending agent.
Example 4
This example provides screening tests for components of a verticillium lecanii dispersible oil suspension.
(1) Emulsifying effect of different dispersing media and emulsifier
Uniformly mixing the dispersion medium and the emulsifier in a vortex mode according to the ratio of 5:1, taking 0.5mL at room temperature of 25 ℃, dripping into a measuring cylinder with a plug and filled with 250mL of standard hard water, observing the dispersibility, shaking up, observing the emulsibility, and recording the emulsifying dispersibility of the preparation, wherein the evaluation standard is as follows:
firstly, the fluorescent blue or light white transparent emulsion can be quickly and automatically uniformly dispersed and slightly stirred; secondly, automatically and uniformly dispersing the mixture into blue or light milky semitransparent emulsion by slight stirring; thirdly, the white cloud is dispersed in a fog shape or a filiform shape, and the white cloud is slightly stirred to form light blue or milky opaque emulsion; fourthly, the white particles sink and form opaque emulsion after stirring; and fifthly, sinking in oil state, incompletely emulsifying under stirring, and quickly demixing after stopping.
The emulsifying effect of different emulsifiers on different dispersion media is shown in table 1.
TABLE 1 emulsification Effect of different dispersion media with emulsifiers
| |
|
OP-10 | Emulsifier A | Emulsifier B | |
| Soybean oil | III | Five of them | II | II | II |
| Sunflower seed oil | Five of them | Five of them | Five of them | III | III |
| Peanut oil | Five of them | Five of them | Five of them | III | III |
| Oleic acid methyl ester | II | Fourthly | II | Fourthly | Fourthly |
As shown in Table 1, different emulsifiers have larger difference in emulsification effect on different dispersion media, OP-10, the emulsifier A and the emulsifier B have better emulsification effect on soybean oil, and span 80 and OP-10 have better emulsification effect on methyl oleate.
(2) Emulsifying effect of different emulsifier compound proportions
Because a single emulsifier is unstable in an OD system and has a common emulsification effect, the emulsifier needs to be compounded, and compounding proportion screening is carried out according to the screening result shown in Table 1, because OP-10 is layered with the emulsifier A and the emulsifier B, the emulsifier A and the emulsifier B are selected as the emulsifiers of soybean oil, and the screening result is shown in Table 2.
TABLE 2 emulsifying effect of different emulsifier combination ratios
As shown in table 2, OP-10, span 80 ═ 3:2, gave better emulsification effect on the methyl oleate system, and emulsifier a and emulsifier B were 4:1, gave better emulsification effect on the soybean oil system.
(3) Dispersing effect of different dispersants
The screened soybean oil was taken as a dispersion medium, 20% of an emulsifier (emulsifier a: emulsifier B: 4:1) and 20% of an emulsifier (OP-10: span 80: 3:2) were added with methyl oleate as a dispersion medium, and then 0.3g of a dispersant was added to observe the dispersion, as shown in table 3.
TABLE 3 dispersing effect of different dispersants
As shown in Table 3, calcium lignosulfonate and Tween 80 were found to be the best dispersants for the soybean oil and methyl oleate systems, respectively.
(4) Protective effect of different UV-protective Agents
The screened soybean oil and methyl oleate are used as dispersion media, deionized water (blank control) with the mass fraction of 1%, benzophenone, sodium fluorescein and ascorbic acid are respectively added as ultraviolet protective agents, spore suspension is prepared from the solution, after the spore suspension is respectively irradiated for 50min under a 312nm ultraviolet lamp, the colony numbers of different treatments are determined by using a dilution plate method, the treatment is repeated for 3 times, and after 48h, the colony numbers are counted by using a counter, wherein the details are shown in table 4.
TABLE 4 protective effect of different UV protectors
As shown in Table 4, fluorescein sodium is the best protective effect as the ultraviolet protective agent of the soybean oil and methyl oleate system.
(5) Screening the components in proportion
After a large number of coarse screening tests, the design of a three-factor three-level response surface is finally determined by using an emulsifier (15% -25%), a dispersant (1% -5%) and a viscosity regulator (1% -3%), and the design factors and levels of the response surface test are shown in table 5.
TABLE 5 response surface test design factors and levels
The design principle of Box-Benhnken (BBD) center combined test is adopted, and the design scheme and the result are shown in Table 6.
TABLE 6 Experimental design of response surface and results
The oil separation rate after being stored at 36 +/-2 ℃ for 7 days is used as a response value regression equation, and the significance is detected to be 0.0017(P <0.01), so that the quadratic equation model is very significant, and the results are shown in table 7.
TABLE 7 analysis of variance of regression equation for response surface test
Performing multiple quadratic regression fitting analysis on the response value in the table 7 by using Design expert11.0 software to establish a multiple quadratic linear regression equation of the OD oil separation rate on independent variables A (dispersing agent), B (emulsifying agent) and C (viscosity regulator) by taking the oil separation rate (Y) as a predicted value:
Y＝42.92+2.50A+2.50B-15.00C+2.50AB+5.50AC-1.50BC+3.38B2+5.62C2
wherein, the model correlation coefficient R20.8590, the model adjustment determination coefficient randj 2 0.6710. And (3) carrying out variance analysis on the model regression equation, wherein the integral model reaches a significant level (P is less than 0.05), and the influence is not significant in a simulation failure item (P is more than 0.05), so that the selected model is suitable and can be used for fitting the test. The effect of the first order term C on the oil extraction rate is more significant, whereas the effects of B and C are not significant. Within the range of the factors taken, the results of the influence of the factors on sporulation yield are ranked as viscosity regulator > emulsifier ═ dispersant.
Multiple quadratic regression fitting is performed on the data in table 7 to obtain a response surface graph and a contour graph of the regression equation, which are shown in fig. 1. As can be seen from FIGS. 1-A and 1-B, the interaction between the emulsifier and the dispersant and the viscosity modifier is significant, and the oil separation rate decreases with the increase of the content of the viscosity modifier; in contrast to FIG. 1-C, the interaction of the dispersant with the emulsifier was not significant.
The optimal formula obtained by analyzing according to Design-Expert 11.0 software is as follows: the emulsifier is 1.25g, the dispersant is 0.05g, the viscosity regulator is 0.15g, the oil separation rate under the condition is 17.24%, the relative error with the predicted value is small, the fitting degree of a regression model and an experimental result is high, a regression equation can truly reflect the influence of the emulsifier, the dispersant and the viscosity regulator on an OD preparation, and the model has feasibility. The above test results were combined to obtain the composition ratio of the verticillium lecanii dispersible oil suspension shown in example 2 and example 3.
Example 5
The embodiment provides a test for performance and effect of the verticillium lecanii dispersible oil suspending agent.
Based on the verticillium lecanii dispersible oil suspending agents provided in the examples 2 and 3, the performance of the dispersible oil suspending agent is tested according to the reference standard shown in the table 8.
TABLE 8 reference standards for dispersible oil suspensions
Based on the reference standards for dispersible oil suspending agent performance shown in table 8, the test results are shown in table 9.
TABLE 9 results of performance index test of verticillium lecanii dispersible oil suspension agent
As shown in table 9, the verticillium lecanii dispersible oil suspension provided in example 2 and example 3 has outstanding performance in dispersible oil suspension, low rate of mixed bacteria, high suspension rate, weak pH, good pourability and good performance in wet sieve test.
Example 6
This example provides a test for determining the storage stability of a verticillium lecanii dispersible oil suspension.
The germination rates of spores in the verticillium lecanii dispersible oil suspension provided in example 2(1#) and example 3(2#) were continuously measured for six months, and the germination rates were all reduced (as shown in fig. 2). Test results show that the verticillium lecanii in the formula of the verticillium lecanii dispersible oil suspending agent provided in the example 3 has better storage stability, CK changes along with time, and the spore germination rate is reduced faster, so that the verticillium lecanii dispersible oil suspending agent provided in the examples 2(1#) and 3(2#) increases the stability of the verticillium lecanii spores.
Example 7
The embodiment provides an indoor toxicity effect test of the verticillium lecanii dispersible oil suspending agent.
The verticillium lecanii dispersible oil suspending agents provided in the example 2 and the example 3 are respectively diluted by 4.87 multiplied by 106cfu/mL、2.44×106cfu/mL、1.22×106cfu/mL、6.09×105cfu/mL、3.04×105The concentration of cfu/mL is 5, the half-finished product (without adding spore powder) is respectively diluted by 100 times, 200 times, 400 times, 800 times and 1600 times to be used as a positive control, and the distilled water is used as a negative control. The toxicity is determined by using an insect soaking method, after medicine is soaked for 5s, the obtained product is placed on fresh leaves in a 24-pore plate (2 mL of water agar culture medium is firstly added into each pore, and after the obtained product is solidified, the broccoli leaves are placed on the upper layer), the obtained product is cultured in an illumination incubator with the temperature of 25 +/-1 ℃, the RH being 80% and the L being 14h and 10h, the number of dead insects is recorded every 24h, 24 times of treatment are carried out, each treatment is repeated for 3 times, LC50 is calculated by using a probability analysis method, and the death rate of the green peach aphids is observed and recorded. The death of the dead insects was scored by touching the body with a writing brush, and the control mortality rate of less than 10% was determined as effective and corrected with the control mortality rate, and the test results are shown in table 10. Wherein, 3.04 is multiplied by 105cfu/mL of the infection effect of Verticillium lecanii dispersible oil suspending agent treatment provided in example 3 is shown in FIG. 3, where FIG. 3-A is 24h after the semi-finished product is applied and FIG. 3-B is 24h after the Verticillium lecanii dispersible oil suspending agent is applied.
TABLE 10 results of performance index test of verticillium lecanii dispersible oil suspension agent
The peach aphids are found to be wrapped with hyphae after being treated with the verticillium lecanii dispersible oil suspending agent provided in the embodiment 2 and the embodiment 3 for 2 days, and are cultured by PDA to be consistent with the form of the verticillium lecanii. In the treatment period of 5d, the death rate of the green peach aphids is more than 90%, which shows that the two preparations can play a better role. The verticillium lecanii dispersible oil suspending agent provided by the embodiment 2 and the embodiment 3 can kill peach aphids after 48 hours, the insecticidal effect is obviously enhanced along with the time extension, and the insecticidal effect is better and LC (liquid crystal) in 120 hours50Are respectively 1.99 multiplied by 105cfu/mL、1.17×105The death rate of cfu/mL is more than 90 percent. In the test, the virulence effects of the two semi-finished preparations are not obviously different from those of CK, but are obviously different from those of the two dispersible oil suspending agents, which shows that verticillium lecanii causes the death of the green peach aphids, and other components are non-insecticidal active substances.
As described above, the present invention can be preferably implemented, and the above-mentioned embodiments only describe the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various changes and modifications of the technical solution of the present invention made by those skilled in the art without departing from the design spirit of the present invention shall fall within the protection scope defined by the present invention.
Claims (10)
1. The verticillium lecanii dispersible oil suspending agent is characterized by comprising verticillium lecanii spore powder, a dispersion medium, an emulsifier, a dispersant, a viscosity regulator and an ultraviolet protective agent, wherein the proportion of the verticillium lecanii spore powder, the dispersion medium, the emulsifier, the dispersant, the viscosity regulator and the ultraviolet protective agent is 5:70:20:1:3:1 in terms of mass ratio.
2. The verticillium lecanii dispersible oil suspension agent of claim 1, wherein the dispersion medium is one of soybean oil and methyl oleate, the emulsifier is one of a combination of vegetable oil emulsifier ZR-5 and soybean oil emulsifier, and a combination of OP-10 and span 80, the dispersant is one of calcium lignosulfonate and Tween-80, the viscosity regulator is organic bentonite, and the ultraviolet protective agent is sodium fluorescein.
3. The verticillium lecanii dispersible oil suspension of claim 1, wherein the dispersion medium is soybean oil, the emulsifier is a combination of vegetable oil emulsifier ZR-5 and soybean oil emulsifier, the dispersant is calcium lignosulfonate, the viscosity modifier is organic bentonite, and the ultraviolet protectant is sodium fluorescein.
4. The verticillium lecanii dispersible oil suspension concentrate of claim 1, wherein the dispersion medium is methyl oleate, the emulsifier is a combination of OP-10 and span 80, the dispersant is Tween-80, the viscosity modifier is organobentonite, and the UV protectant is sodium fluorescein.
5. The Verticillium lecanii dispersible oil suspending agent according to claim 3, wherein the ratio of the vegetable oil emulsifier ZR-5 to the soybean oil emulsifier is 3: 2-4: 1 by mass ratio.
6. The verticillium lecanii dispersible oil suspending agent of claim 4, wherein the proportion of OP-10 and span 80 is 1: 1-3: 2 by mass ratio.
7. The process for preparing the verticillium lecanii dispersible oil suspension agent of claim 1, which comprises: weighing a dispersion medium, an emulsifier, a dispersant, a viscosity regulator and an ultraviolet protective agent, mixing and shearing, and then adding verticillium lecanii spore powder under an ice bath condition to uniformly shear to obtain the verticillium lecanii dispersible oil suspending agent;
the dispersion medium is soybean oil, the emulsifier is a combination of a vegetable oil emulsifier ZR-5 and a soybean oil emulsifier, the dispersant is calcium lignosulfonate, the viscosity regulator is organic bentonite, and the ultraviolet protective agent is fluorescein sodium; the ratio of the vegetable oil emulsifier ZR-5 to the soybean oil emulsifier is 3: 2-4: 1 by mass ratio.
8. The process for preparing the verticillium lecanii dispersible oil suspension agent of claim 1, which comprises: weighing a dispersion medium, an emulsifier, a dispersant, a viscosity regulator and an ultraviolet protective agent, mixing and shearing, and then adding verticillium lecanii spore powder under an ice bath condition to uniformly shear to obtain the verticillium lecanii dispersible oil suspending agent;
the dispersing medium is methyl oleate, the emulsifier is a combination of OP-10 and span 80, the dispersant is Tween-80, the viscosity regulator is organic bentonite, and the ultraviolet protective agent is fluorescein sodium; the mass ratio of the OP-10 to the span 80 is 1: 1-3: 2.
9. The application of the verticillium lecanii dispersible oil suspending agent as defined in any one of claims 1-6 in the aspect of killing insects.
10. The use of claim 7, wherein the verticillium lecanii dispersible oil suspension is used for killing myzus persicae.
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| CN106665181A (en) * | 2017-01-10 | 2017-05-17 | 刘继强 | Imitated-skin water-absorbing breathable charcoal cup and preparation method thereof |
| US20210127686A1 (en) * | 2018-06-15 | 2021-05-06 | Bayer Aktiengesellschaft | Liquid and storage-stable formulations for fungal spores |
| WO2020206518A1 (en) * | 2019-04-10 | 2020-10-15 | Agrivalle Brasil Industria E Comércio De Produtos Agrícolas Ltda | Multipurpose biological compositions |
| WO2021221690A1 (en) * | 2020-05-01 | 2021-11-04 | Pivot Bio, Inc. | Modified bacterial strains for improved fixation of nitrogen |
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