CN114303793A - Sparassis crispa culture medium and cultivation method thereof - Google Patents
Sparassis crispa culture medium and cultivation method thereof Download PDFInfo
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- 239000001963 growth medium Substances 0.000 title claims abstract description 32
- 238000012364 cultivation method Methods 0.000 title claims abstract description 14
- 241000272503 Sparassis radicata Species 0.000 title claims description 43
- 238000000034 method Methods 0.000 claims abstract description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 40
- 239000000463 material Substances 0.000 claims description 39
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 26
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 20
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 20
- 238000005286 illumination Methods 0.000 claims description 18
- 239000000843 powder Substances 0.000 claims description 18
- 230000001954 sterilising effect Effects 0.000 claims description 18
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 17
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 14
- 235000003704 aspartic acid Nutrition 0.000 claims description 14
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims description 14
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 14
- 240000008042 Zea mays Species 0.000 claims description 13
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 13
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 13
- 235000005822 corn Nutrition 0.000 claims description 13
- 235000013312 flour Nutrition 0.000 claims description 13
- 239000010440 gypsum Substances 0.000 claims description 13
- 229910052602 gypsum Inorganic materials 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 235000015099 wheat brans Nutrition 0.000 claims description 13
- 239000000292 calcium oxide Substances 0.000 claims description 12
- 235000012255 calcium oxide Nutrition 0.000 claims description 12
- 238000003306 harvesting Methods 0.000 claims description 12
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 12
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 12
- 238000011081 inoculation Methods 0.000 claims description 11
- 238000012258 culturing Methods 0.000 claims description 10
- 235000012343 cottonseed oil Nutrition 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 8
- 239000011691 vitamin B1 Substances 0.000 claims description 8
- 239000002023 wood Substances 0.000 claims description 8
- 229920000742 Cotton Polymers 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 230000005855 radiation Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 2
- 239000000853 adhesive Substances 0.000 claims 2
- 230000001070 adhesive effect Effects 0.000 claims 2
- 235000014486 Hydrangea macrophylla Nutrition 0.000 abstract description 4
- 244000005700 microbiome Species 0.000 abstract description 2
- 241001092080 Hydrangea Species 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 12
- 235000015073 liquid stocks Nutrition 0.000 description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 9
- 239000008103 glucose Substances 0.000 description 9
- 239000007836 KH2PO4 Substances 0.000 description 6
- 239000001888 Peptone Substances 0.000 description 6
- 108010080698 Peptones Proteins 0.000 description 6
- 244000061456 Solanum tuberosum Species 0.000 description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 description 6
- 235000019319 peptone Nutrition 0.000 description 6
- 239000011782 vitamin Substances 0.000 description 5
- 229930003231 vitamin Natural products 0.000 description 5
- 235000013343 vitamin Nutrition 0.000 description 5
- 229940088594 vitamin Drugs 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 235000019733 Fish meal Nutrition 0.000 description 3
- 244000267823 Hydrangea macrophylla Species 0.000 description 3
- 230000003213 activating effect Effects 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 238000009835 boiling Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000004467 fishmeal Substances 0.000 description 3
- 238000009630 liquid culture Methods 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000222383 Polyporales Species 0.000 description 1
- 241000123261 Sparassidaceae Species 0.000 description 1
- 241000123241 Sparassis Species 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
Images
Abstract
The invention is applicable to the technical field of microorganisms, and provides a hydrangea culture medium and a cultivation method thereof. The method has the advantages of simple operation, low cost and high hypha growth speed, can effectively recover products and generates good benefits.
Description
Technical Field
The invention belongs to the field of microorganisms, and particularly relates to a Sparassis crispa culture medium and a cultivation method thereof.
Background
Sparassis crispa (Sparassis crispa), also called Sparassis crispa and Sparassis crispa, belonging to the Basidiomycotina, Isobasidiomycetes, Aphyllophorales, Sparassidaceae and Sparassis, and is named because it is shaped like giant Sparassis crispa.
Sparassis crispa is used as a rare edible and medicinal dual-purpose bacterium, wild resources are rare, artificial cultivation is realized, but the preparation time of Sparassis crispa mother strains is long due to slow growth speed of hypha and low biomass. How to promote the growth of hypha and improve the biomass becomes a key problem which restricts the industrial development of the Sparassis crispa.
Disclosure of Invention
The invention provides a Sparassis crispa culture medium and a cultivation method thereof, and aims to solve the problems in the prior art.
The invention is realized in such a way that, on one hand, the invention provides a Sparassis crispa culture medium, which comprises the following components in parts by mass: 55-65 parts of miscellaneous tree sawdust, 20-30 parts of cottonseed hull, 10-15 parts of wheat bran, 5-15 parts of corn flour, 1-2 parts of gypsum powder, 1-2 parts of quicklime, 0.1-0.2 part of monopotassium phosphate, 0.1-0.2 part of aspartic acid, 0.1-0.2 part of magnesium sulfate, vitamin B10.0005 to 0.0015 portion.
Preferably, the Sparassis crispa culture medium comprises the following components in parts by mass: 50 parts of mixed wood chips, 25 parts of cottonseed hulls, 12 parts of wheat bran, 5 parts of corn flour, 1.5 parts of gypsum powder, 1.3 parts of quicklime, 0.15 part of monopotassium phosphate, 0.15 part of aspartic acid, 0.15 part of magnesium sulfate, and vitamin B10.0012 part.
On the other hand, the invention also provides a method for cultivating the sparassis crispa, which comprises the following steps:
s1, preparing culture materials: weighing appropriate amount of miscellaneous sawdust, cotton seed hull, testa Tritici, semen Maydis powder, Gypsum Fibrosum powder, calx, potassium dihydrogen phosphate, aspartic acid, magnesium sulfate, and vitamin B according to any one of the above formulas1Adding water, and uniformly stirring to obtain the product with the water content of 60-65 percentCulturing materials;
s2, sterilization: sterilizing the culture materials obtained in the step S1, cooling the culture materials to room temperature, and subpackaging the culture materials in culture bags, wherein the mass of the culture materials in each culture bag is 0.6 kg-0.75 kg;
s3, inoculation; inoculating in a sterile room;
s4, cultivation: placing the culture bag into a culture chamber, and firstly keeping the temperature in the culture chamber at 24-26 ℃; when the hypha covers the surface of the culture material, the temperature in the culture chamber is kept to be 22-24 ℃; in the first 30 days of culture, the illumination in the culture room is adjusted to be no light or weak light; keeping the culture room for 300-500 luxes 35 days after the beginning of the culture;
s5, fruiting: after one month, when the primordia appear, transferring the culture bag to a fruiting room; then, within 30 days, controlling the temperature to be 21-23 ℃, keeping the relative humidity of air at 75-85%, and keeping the illumination at the heat radiation light of 500-800 lux; after 30 days of entering the fruiting room, adjusting the air humidity to 90-95%, and adjusting the illumination intensity to 800-1000 lux; continuously culturing for 30 days to expose the fruiting body, and continuously culturing for 30 days;
s6, harvesting: and (4) harvesting according to the development status of sparassis crispa sporocarp.
Compared with the prior art, the invention has the beneficial effects that: the Sparassis crispa culture medium and the cultivation method thereof adopt miscellaneous sawdust, cottonseed hulls, wheat bran, corn flour, gypsum powder, quicklime, monopotassium phosphate, aspartic acid, magnesium sulfate and vitamins as culture materials, and obtain the Sparassis crispa with high yield and high quality by preparing the culture materials, sterilizing, inoculating, cultivating, fruiting and harvesting in sequence. The method has the advantages of simple operation, low cost and high hypha growth speed, can effectively recover products and generates good benefits.
Drawings
FIG. 1 is a schematic flow chart of a method for cultivating Sparassis crispa according to the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
The embodiment provides a technical scheme: a hydrangea culture medium and a cultivation method thereof are provided, wherein the culture medium comprises the following components by mass: 50 parts of mixed wood chips, 25 parts of cottonseed hulls, 12 parts of wheat bran, 5 parts of corn flour, 1.5 parts of gypsum powder, 1.3 parts of quicklime, 0.15 part of monopotassium phosphate, 0.15 part of aspartic acid, 0.15 part of magnesium sulfate, and vitamin B10.0012 part.
Referring to fig. 1, the cultivation method includes the following steps: before cultivation, mother seeds need to be prepared:
(1) activating the mother seeds. Inoculating Sparassis crispa to PDA (peeled potato 200g, glucose 20g, agar powder 16g) slant culture medium, and culturing at 23-25 deg.C for 25 days to obtain activated Sparassis crispa mother strain; (2) preparing a liquid mother seed. Peeling potato 200g, slicing, adding water 1L, boiling, filtering, adding glucose 20g, peptone 2g, KH2PO43g，MgSO4·7H2O1g, uniformly stirring, fixing the volume to 1L, and adjusting the pH to 5.5; (3) subpackaging the liquid culture medium into 250ml triangular flasks, filling the liquid into the flasks with the volume of 100ml, and sterilizing for 30min by high-pressure steam; (4) sterilizing, inoculating 5-8 blocks (0.5cm × 0.5cm) of activated slant mother strain, and culturing in shaker at 150rpm and 23-25 deg.C for 10 days to obtain liquid mother strain.
Stock preparation using the mother seed is also required: (1) preparing a liquid stock culture medium: 20g of glucose, 6g of fish meal peptone and KH2PO45g，MgSO4·7H2O3g, initial pH 5. (2) And inoculating the cultured liquid mother culture medium into the liquid stock culture medium, wherein the inoculation amount is 8%, the culture temperature is 23-25 ℃, the shaking rotation speed is 150rpm, and the shaking culture is carried out for 15 days under the natural illumination condition, so as to obtain the sparassis crispa liquid stock. Sparassis crispa liquid stock is inoculated into the culture bag of the example for cultivation of Sparassis crispa.
S1, preparing culture materials: weighing a proper amount of miscellaneous wood chips, cotton seed hulls, wheat bran, corn flour, gypsum powder, quicklime, monopotassium phosphate, aspartic acid, magnesium sulfate and vitamins according to the formula, adding water, and uniformly stirring to obtain the culture material with the water content of 60-65%.
S2, sterilization: and (4) sterilizing the culture materials obtained in the step (S1), cooling the culture materials to room temperature, and subpackaging the culture materials in culture bags, wherein the mass of the culture materials in each culture bag is 0.6 kg-0.75 kg. The culture bag has a size of 150X 300mm X0.05 mm.
S3, inoculation; the inoculation operation is carried out in a sterile room.
S4, cultivation: placing the culture bag into a culture chamber, and firstly keeping the temperature in the culture chamber at 24-26 ℃; when the hypha covers the surface of the culture material, the temperature in the culture chamber is kept to be 22-24 ℃; in the first 30 days of culture, the illumination in the culture room is adjusted to be no light or weak light; the culture is maintained in the culture chamber for 300 to 500 luxes 35 days after the start of the culture.
S5, fruiting: after one month, when the primordia appear, transferring the culture bag to a fruiting room; then, within 30 days, controlling the temperature to be 21-23 ℃, keeping the relative humidity of air at 75-85%, and keeping the illumination at the heat radiation light of 500-800 lux; after 30 days of entering the fruiting room, adjusting the air humidity to 90-95%, and adjusting the illumination intensity to 800-1000 lux; the culture was continued for 30 days to expose the fruiting bodies to the outside, and then the culture was continued for 30 days.
S6, harvesting: and (4) harvesting according to the development status of sparassis crispa sporocarp.
Example 2
The embodiment provides a technical scheme: a hydrangea culture medium and a cultivation method thereof are provided, wherein the culture medium comprises the following components by mass: 55 parts of mixed wood chips, 20 parts of cottonseed hulls, 15 parts of wheat bran, 10 parts of corn flour, 2 parts of gypsum powder, 2 parts of quicklime, 0.2 part of monopotassium phosphate, 0.2 part of aspartic acid, 0.2 part of magnesium sulfate and vitamin B10.0012 part.
Referring to fig. 1, the cultivation method includes the following steps:
before cultivation, mother seeds need to be prepared:
(1) activating the mother seeds. Inoculating Sparassis crispa to PDA (peeled potato 200g, glucose 20g, agar powder 16g) slant culture medium, and culturing at 23-25 deg.C for 25 days to obtain activated Sparassis crispa mother strain; (2) preparing a liquid mother seed. Peeling potato 200g, slicing, adding water 1L, boiling, filtering, adding glucose 20g, peptone 2g, KH2PO43g，MgSO4·7H2O1g, uniformly stirring, fixing the volume to 1L, and adjusting the pH to 5.5; (3) subpackaging the liquid culture medium into 250ml triangular flasks, filling the liquid into the flasks with the volume of 100ml, and sterilizing for 30min by high-pressure steam; (4) sterilizing, inoculating 5-8 blocks (0.5cm × 0.5cm) of activated slant mother strain, and culturing in shaker at 150rpm and 23-25 deg.C for 10 days to obtain liquid mother strain.
Stock preparation using the mother seed is also required: (1) preparing a liquid stock culture medium: 20g of glucose, 6g of fish meal peptone and KH2PO45g，MgSO4·7H2O3g, initial pH 5. (2) And inoculating the cultured liquid mother culture medium into the liquid stock culture medium, wherein the inoculation amount is 8%, the culture temperature is 23-25 ℃, the shaking rotation speed is 150rpm, and the shaking culture is carried out for 15 days under the natural illumination condition, so as to obtain the sparassis crispa liquid stock. Sparassis crispa liquid stock is inoculated into the culture bag of the example for cultivation of Sparassis crispa.
S1, preparing culture materials: weighing a proper amount of miscellaneous wood chips, cotton seed hulls, wheat bran, corn flour, gypsum powder, quicklime, monopotassium phosphate, aspartic acid, magnesium sulfate and vitamins according to the formula, adding water, and uniformly stirring to obtain the culture material with the water content of 60-65%.
S2, sterilization: and (4) sterilizing the culture materials obtained in the step (S1), cooling the culture materials to room temperature, and subpackaging the culture materials in culture bags, wherein the mass of the culture materials in each culture bag is 0.6 kg-0.75 kg. The culture bag has a size of 150X 300mm X0.05 mm.
S3, inoculation; the inoculation operation is carried out in a sterile room.
S4, cultivation: placing the culture bag into a culture chamber, and firstly keeping the temperature in the culture chamber at 24-26 ℃; when the hypha covers the surface of the culture material, the temperature in the culture chamber is kept to be 22-24 ℃; in the first 30 days of culture, the illumination in the culture room is adjusted to be no light or weak light; the culture is maintained in the culture chamber for 300 to 500 luxes 35 days after the start of the culture.
S5, fruiting: after one month, when the primordia appear, transferring the culture bag to a fruiting room; then, within 30 days, controlling the temperature to be 21-23 ℃, keeping the relative humidity of air at 75-85%, and keeping the illumination at the heat radiation light of 500-800 lux; after 30 days of entering the fruiting room, adjusting the air humidity to 90-95%, and adjusting the illumination intensity to 800-1000 lux; the culture was continued for 30 days to expose the fruiting bodies to the outside, and then the culture was continued for 30 days.
S6, harvesting: and (4) harvesting according to the development status of sparassis crispa sporocarp.
Example 3
The embodiment provides a technical scheme: a hydrangea culture medium and a cultivation method thereof are provided, wherein the culture medium comprises the following components by mass: 65 parts of mixed wood chips, 20 parts of cottonseed hulls, 10 parts of wheat bran, 10 parts of corn flour, 1 part of gypsum powder, 1 part of quicklime, 0.1 part of monopotassium phosphate, 0.1 part of aspartic acid, 0.1 part of magnesium sulfate and vitamin B10.0005 portion.
Referring to fig. 1, the cultivation method includes the following steps:
before cultivation, mother seeds need to be prepared:
(1) activating the mother seeds. Inoculating Sparassis crispa to PDA (peeled potato 200g, glucose 20g, agar powder 16g) slant culture medium, and culturing at 23-25 deg.C for 25 days to obtain activated Sparassis crispa mother strain; (2) preparing a liquid mother seed. Peeling potato 200g, slicing, adding water 1L, boiling, filtering, adding glucose 20g, peptone 2g, KH2PO43g，MgSO4·7H2O1g, uniformly stirring, fixing the volume to 1L, and adjusting the pH to 5.5; (3) subpackaging the liquid culture medium into 250ml triangular flasks, filling the liquid into the flasks with the volume of 100ml, and sterilizing for 30min by high-pressure steam; (4) sterilizing, inoculating 5-8 blocks (0.5cm × 0.5cm) of activated slant mother strain, and culturing in shaker at 150rpm and 23-25 deg.C for 10 days to obtain liquid mother strain.
Stock preparation using the mother seed is also required: (1) preparing a liquid stock culture medium: 20g of glucose, 6g of fish meal peptone and KH2PO45g，MgSO4·7H2O3g, initial pH 5. (2) And inoculating the cultured liquid mother culture medium into the liquid stock culture medium, wherein the inoculation amount is 8%, the culture temperature is 23-25 ℃, the shaking rotation speed is 150rpm, and the shaking culture is carried out for 15 days under the natural illumination condition, so as to obtain the sparassis crispa liquid stock. Sparassis crispa liquid stock is inoculated into the culture bag of the example for cultivation of Sparassis crispa.
S1, preparing culture materials: weighing a proper amount of miscellaneous wood chips, cotton seed hulls, wheat bran, corn flour, gypsum powder, quicklime, monopotassium phosphate, aspartic acid, magnesium sulfate and vitamins according to the formula, adding water, and uniformly stirring to obtain the culture material with the water content of 60-65%.
S2, sterilization: and (4) sterilizing the culture materials obtained in the step (S1), cooling the culture materials to room temperature, and subpackaging the culture materials in culture bags, wherein the mass of the culture materials in each culture bag is 0.6 kg-0.75 kg. The culture bag has a size of 150X 300mm X0.05 mm.
S3, inoculation; the inoculation operation is carried out in a sterile room.
S4, cultivation: placing the culture bag into a culture chamber, and firstly keeping the temperature in the culture chamber at 24-26 ℃; when the hypha covers the surface of the culture material, the temperature in the culture chamber is kept to be 22-24 ℃; in the first 30 days of culture, the illumination in the culture room is adjusted to be no light or weak light; the culture is maintained in the culture chamber for 300 to 500 luxes 35 days after the start of the culture.
S5, fruiting: after one month, when the primordia appear, transferring the culture bag to a fruiting room; then, within 30 days, controlling the temperature to be 21-23 ℃, keeping the relative humidity of air at 75-85%, and keeping the illumination at the heat radiation light of 500-800 lux; after 30 days of entering the fruiting room, adjusting the air humidity to 90-95%, and adjusting the illumination intensity to 800-1000 lux; the culture was continued for 30 days to expose the fruiting bodies to the outside, and then the culture was continued for 30 days.
S6, harvesting: and (4) harvesting according to the development status of sparassis crispa sporocarp.
The Sparassis crispa culture medium and the cultivation method thereof adopt miscellaneous sawdust, cottonseed hulls, wheat bran, corn flour, gypsum powder, quicklime, monopotassium phosphate, aspartic acid, magnesium sulfate and vitamins as culture materials, and obtain the Sparassis crispa with high yield and high quality by preparing the culture materials, sterilizing, inoculating, cultivating, fruiting and harvesting in sequence. The method has the advantages of simple operation, low cost and high hypha growth speed, can effectively recover products and generates good benefits.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (3)
1. A Sparassis crispa culture medium is characterized in that: the adhesive comprises the following components in parts by weight: 55-65 parts of miscellaneous tree sawdust, 20-30 parts of cottonseed hull, 10-15 parts of wheat bran, 5-15 parts of corn flour, 1-2 parts of gypsum powder, 1-2 parts of quicklime, 0.1-0.2 part of monopotassium phosphate, 0.1-0.2 part of aspartic acid, 0.1-0.2 part of magnesium sulfate, vitamin B10.0005 to 0.0015 portion.
2. The Sparassis crispa culture medium of claim 1, wherein: the adhesive comprises the following components in parts by weight: 50 parts of mixed wood chips, 25 parts of cottonseed hulls, 12 parts of wheat bran, 5 parts of corn flour, 1.5 parts of gypsum powder, 1.3 parts of quicklime, 0.15 part of monopotassium phosphate, 0.15 part of aspartic acid, 0.15 part of magnesium sulfate, and vitamin B10.0012 part.
3. A sparassis crispa cultivation method is characterized in that: the method comprises the following steps:
s1, preparing culture materials: weighing appropriate amount of miscellaneous sawdust, cotton seed hull, wheat bran, corn flour, gypsum powder, calx, potassium dihydrogen phosphate, aspartic acid, magnesium sulfate, vitamin B1Adding water, and stirring to obtain water content of 60%65% of culture material;
s2, sterilization: sterilizing the culture materials obtained in the step S1, cooling the culture materials to room temperature, and subpackaging the culture materials in culture bags, wherein the mass of the culture materials in each culture bag is 0.6 kg-0.75 kg;
s3, inoculation; inoculating in a sterile room;
s4, cultivation: placing the culture bag into a culture chamber, and firstly keeping the temperature in the culture chamber at 24-26 ℃; when the hypha covers the surface of the culture material, the temperature in the culture chamber is kept to be 22-24 ℃; in the first 30 days of culture, the illumination in the culture room is adjusted to be no light or weak light; keeping the culture room for 300-500 luxes 35 days after the beginning of the culture;
s5, fruiting: after one month, when the primordia appear, transferring the culture bag to a fruiting room; then, within 30 days, controlling the temperature to be 21-23 ℃, keeping the relative humidity of air at 75-85%, and keeping the illumination at the heat radiation light of 500-800 lux; after 30 days of entering the fruiting room, adjusting the air humidity to 90-95%, and adjusting the illumination intensity to 800-1000 lux; continuously culturing for 30 days to expose the fruiting body, and continuously culturing for 30 days;
s6, harvesting: and (4) harvesting according to the development status of sparassis crispa sporocarp.
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| CN112889578A (en) * | 2021-01-18 | 2021-06-04 | 陕西科技大学 | Thelephora ganbajun zang strain and preparation method and application thereof |
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2021
- 2021-12-09 CN CN202111496780.XA patent/CN114303793A/en active Pending
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| CN101955392A (en) * | 2010-09-19 | 2011-01-26 | 福建省农业科学院食用菌研究所 | Formula of culture medium for industrial production of sparasis crispa and production process |
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