CN114295853A - Full-automatic chemiluminescence immunoassay analyzer and application method thereof - Google Patents

Abstract

The invention relates to the technical field of chemiluminescence immunoassay equipment, and particularly discloses a full-automatic chemiluminescence immunoassay analyzer and a using method thereof, wherein the full-automatic chemiluminescence immunoassay analyzer comprises a box body, photomultiplier tubes are fixed on two sides of the box body, a plurality of reagent tubes are arranged on one side of the box body, a conveying assembly is fixed on one side of the box body, a pushing assembly is arranged at the top of the conveying assembly, a positioning frame is fixed at the bottom of the box body, a third motor is fixed on the inner wall of the positioning frame, a second gear disc is connected with an output shaft of the third motor, a first gear disc is meshed with the circumferential outer wall of the second gear disc, a rotating rod is fixed on the first gear disc, a connecting disc is connected on the rotating rod, a plurality of second tooth blocks are fixed on the connecting disc, a connecting ring is arranged on the connecting ring, a plurality of placing grooves are arranged on the bottom of the connecting ring, a sleeve is clamped in the positioning grooves, and a first tooth block is connected on the sleeve, the bottom of the positioning groove is connected with a friction gasket. The invention has the characteristics of full reagent mixing and good detection accuracy.

Description

Full-automatic chemiluminescence immunoassay analyzer and application method thereof

Technical Field

The invention relates to the technical field of chemiluminescence immunoassay equipment, in particular to a full-automatic chemiluminescence immunoassay analyzer and a using method thereof.

Background

Chemiluminescence immunoassay comprises two systems of immunoassay and chemiluminescence immunoassay. The immunoassay system directly marks chemiluminescent substances or enzymes on antigens or antibodies as markers, and forms antigen-antibody immune complexes through the reaction of the antigens and the antibodies. The chemiluminescence analysis system is characterized in that after the immune reaction is finished, a luminous substrate of an oxidant or an enzyme is added, a chemiluminescent substance is oxidized by the oxidant to form an intermediate in an excited state, photons are emitted to release energy so as to return to a stable ground state, and the luminous intensity can be detected by a luminous signal measuring instrument (a photomultiplier). According to the relation between the chemiluminescence marker and the luminescence intensity, the content of the detected object can be calculated by using a standard curve.

The chinese patent of invention with publication number CN107831326A in the prior art discloses a full-automatic chemiluminescence immunoassay analyzer, which comprises: the frame body comprises a support frame, a first workbench and a second workbench which are arranged in a layered manner in the vertical direction of the support frame and are mutually communicated; the functional workgroup and the reaction cup storage area are respectively arranged on the second workbench, and the functional workgroup comprises an incubation area, a cleaning area and a detection area; the liquid taking work station group is arranged on the first workbench and comprises a sample area, a reagent area and a gun head storage area for storing the gun head of the liquid transfer gun; a control system; the conveying mechanism is arranged on the frame body, can mix the sample and the reagent obtained from the sample area and the reagent area in the reaction cup under the control of the control system to obtain the object to be detected, and sequentially conveys the object to be detected to the incubation area, the cleaning area and the detection area. The invention can overcome the defects of large occupied area and low efficiency of the full-automatic chemiluminescence immunoassay analyzer in the prior art, thereby providing the full-automatic chemiluminescence immunoassay analyzer with small occupied area and high efficiency.

However, the above device still has the following disadvantages that the incubation area arranged inside the device needs to be heated by external heat energy in the process of heating the reagent, so that the temperature of the reagent box close to the heat source is higher, and the reagent box far away from the heat source cannot obtain more heat, so that the reagent box is heated unevenly, and the reagent box cannot be mixed one by one in the process of heating the reagent in the incubation area, so that the reagent in the reagent box cannot fully react to generate precipitation, thereby affecting the subsequent detection work of the reagent.

Disclosure of Invention

The invention provides a full-automatic chemiluminescence immunoassay analyzer with sufficient reagent mixing and good detection accuracy and a using method thereof, aiming at solving the technical problems of the existing chemiluminescence immunoassay.

The first technical scheme of the invention is as follows: a full-automatic chemiluminescence immunoassay analyzer comprises a box body, wherein a control panel is fixedly connected to the outer wall of one side of the box body, photomultiplier tubes are fixedly connected to the inner walls of the two sides of the box body, a plurality of reagent tubes are arranged on one side of the box body, a conveying assembly for driving the reagent tubes to do horizontal motion is fixedly connected to one side of the box body, a material pushing assembly for feeding the reagent tubes is arranged at the top of the conveying assembly, a positioning frame is fixedly connected to the inner wall of the bottom of the box body, a third motor is fixedly connected to the inner wall of the circumference of the positioning frame, a second gear disc is fixedly connected to one end of an output shaft of the third motor, a first gear disc is meshed with the outer wall of the circumference of the second gear disc, a rotating rod is fixedly connected to the inner wall of the circumference of the first gear disc, and a connecting disc is fixedly connected to one end of the rotating rod, the reagent tube comprises a connecting disc, a plurality of second tooth blocks, a connecting ring, a plurality of placing grooves, a positioning groove and a sleeve, wherein the second tooth blocks are circularly distributed on the outer wall of the connecting disc, the connecting ring is arranged on the outer wall of the connecting ring, the placing grooves are circularly distributed on the outer wall of the top of the connecting ring, the positioning groove is formed in the inner wall of the bottom of each placing groove, the sleeve is connected to the inner wall of the positioning groove, the first tooth blocks are fixedly connected to the outer wall of the sleeve, the first tooth blocks are meshed with the second tooth blocks, the reagent tube is inserted into the inner portion of the sleeve, friction gaskets are fixedly connected to the inner wall of the bottom of each positioning groove, and the inner wall of the bottom of the reagent tube is in contact with the friction gaskets. According to the reagent tube automatic mixing device, when the reagent tube is transferred into the sleeve through the conveying assembly and the pushing assembly until the bottom of the reagent tube is contacted with the friction gasket, a worker starts the third motor, the third motor drives the second gear disc to rotate, the second gear disc is meshed with the first gear disc in the process to drive the rotating rod to rotate, the connecting disc can rotate in the rotating process of the rotating rod, and the second gear block arranged on the circumferential outer wall of the connecting disc is meshed with the first gear block on the outer wall of the sleeve, so that the sleeve can rotate in the placing groove in the rotating process of the connecting disc, reagents in the reagent tube can be fully mixed through the rotation of the sleeve, and the accuracy of a subsequent detection result is improved; the invention has better effect of revolution mixing around the connecting disc through the rotation of the sleeve, and the condition that the reagent spills out from the inside of the reagent tube can be led out due to overlarge centrifugal force when the sleeve is subjected to revolution mixing; in the process of autorotation of the sleeve, the bottom of the reagent tube is in contact with the top of the friction gasket, so that a certain speed reduction effect can be achieved on the sleeve through the friction gasket, the phenomenon that the reagent spills out of the reagent tube due to over-fast rotation of the sleeve is avoided, in addition, in the process of rotation of the reagent tube, the bottom of the reagent tube can be in contact with the friction gasket to generate heat, a good greenhouse environment can be provided for the reagent, the reagent can better react, and because the rotation force applied to each reagent tube is the same, the heat applied to each reagent tube can be ensured to be the same, and the error of subsequent sample detection is greatly reduced.

Preferably, the conveying assembly comprises a sealing cover fixedly connected with the outer wall of one side of the box body, a fixing frame is fixedly connected with the outer wall of one side of the sealing cover, one end of the fixing frame far away from the sealing cover is fixedly connected with a mounting plate, a second motor is fixedly connected with the outer wall of one side of the mounting plate, one end of an output shaft of the second motor is fixedly connected with a driving roller, the circumferential outer wall of the transmission roller is in transmission connection with a transmission belt, the outer wall of one side of the box body is provided with an opening, the inner wall of the bottom of the opening is fixedly connected with a connecting groove, the outer wall of the bottom of the transmission belt is contacted with the inner wall of the bottom of the connecting groove, the top of sealed cowling is provided with the liquid feeding subassembly, the both sides inner wall of spread groove all is provided with direction subassembly, another one side outer wall fixedly connected with reinforcing frame of mounting panel, one side fixed connection of reinforcing frame is in on one side outer wall of spread groove. When the staff need examine reagent, the staff can place the reagent pipe in the spread groove inside and start the second motor, and the second motor drives the driving roller and rotates, can drive driving belt through the rotation of driving roller and rotate together, and driving belt contacts with the bottom of spread groove, consequently can drive the reagent pipe and be horizontal motion.

Preferably, the liquid adding assembly comprises a liquid storage tank and a reagent tank which are fixedly connected to the outer wall of the top of the sealing cover, liquid guide pipes are inserted into the bottoms of the liquid storage tank and the reagent tank, a shell is inserted into one end of each liquid guide pipe, a liquid discharge pipe is inserted into the bottom of the shell and located right above the reagent pipes, and a mixing assembly is arranged inside the shell. When the conveying assembly moves the reagent tube to the position right below the liquid discharge tube, the sample and the reagent in the reagent box and the liquid storage box can be guided into the shell through the liquid guide tube.

Preferably, the mixing assembly is connected including rotating the rotation post of casing circumference inner wall, the circumference outer wall fixedly connected with equidistance of rotation post is the flabellum of circular distribution, the through-hole has been seted up to one side of flabellum, the fixed ware of one side outer wall fixedly connected with of through-hole, fixed ware is the arc structure. The in-process inside leading-in casing of reagent can take place the striking with the flabellum for the flabellum carries out the rotation work, and through set up the through-hole in one side of flabellum and set up fixed ware, can the striking degree of depth of effectual increase mixed reagent and flabellum, has further improved the atress effect of flabellum, makes the rotation that the flabellum can be better carry out mixed work to sample and reagent, has further reduced subsequent detection error.

Preferably, the guide assembly comprises guide plates fixedly connected to the inner walls of the two sides of the connecting groove, the two guide plates are distributed on the two sides of the reagent tube at equal intervals, the inner walls of the two sides of the guide plates are rotatably connected with guide columns, and the circumferential outer walls of the guide columns are in contact with the circumferential outer walls of the reagent tube. When the reagent tube is conveyed to one end of the connecting groove, the reagent tube can be accurately moved to the position under the material pushing assembly through the two groups of guide plates and the guide columns.

As preferred, the material pushing assembly comprises a partition plate fixedly connected to the inner walls of the two sides of the box body, a first through groove is formed in the outer wall of one side of the partition plate, a transverse plate is fixedly connected to the inner wall of the top of the first through groove, a driving assembly is fixedly connected to the outer wall of the bottom of the transverse plate, a limiting assembly is arranged at the bottom of the transverse plate, and the limiting assembly is fixedly connected with the driving assembly.

Preferably, the driving assembly comprises a vertical plate fixedly connected to the outer wall of the bottom of the transverse plate, a first motor is fixedly connected to the outer wall of one side of the vertical plate, a threaded screw rod is fixedly connected to one end of an output shaft of the first motor, a threaded sleeve is meshed to the outer wall of the circumference of the threaded screw rod, an electric push rod is fixedly connected to the outer wall of the bottom of the threaded sleeve, a sealing cover is fixedly connected to the output end of the electric push rod, and the sealing cover is matched with the reagent tube in specification. When reagent pipe passed through conveying assembly and removed under the sealed lid start first motor and electric putter simultaneously, first motor drives the screw lead screw and rotates, can drive screw sleeve and sealed lid through the screw lead screw and remove to reagent pipe directly over, electric putter drives sealed lid and descends until it with reagent pipe looks joint afterwards, restart first motor and electric putter at this moment remove reagent pipe to the inside of the sleeve pipe to full-automatic material loading work to reagent pipe has been realized.

Preferably, the limiting assembly comprises a sliding groove formed in the outer wall of the bottom of the transverse plate, sliding blocks are connected to the inner walls of the two sides of the sliding groove in a sliding mode, an inclined rod is fixedly connected to the outer wall of the bottom of the sliding block, and one end, far away from the sliding block, of the inclined rod is fixedly connected with the threaded sleeve. When the threaded sleeve rotates along with the rotation of the threaded screw rod, the sliding block slides inside the sliding groove, so that the threaded sleeve can be well limited, and the threaded sleeve can stably move horizontally.

Preferably, a second through groove is formed in one side of the sealing cover, a third through groove is formed in the bottom of the box body, the connecting groove penetrates through the second through groove, and the transmission belt penetrates through the third through groove.

Preferably, the reagent tubes are distributed at equal intervals; the plurality of second tooth blocks are distributed at equal intervals; the plurality of placing grooves are distributed at equal intervals; the reagent tubes are distributed at the top of the transmission belt at equal intervals; the cross section of the liquid guide pipe is of a broken line type; the cross section of the shell is spherical.

The second technical scheme of the invention is as follows: a method for using a full-automatic chemiluminescence immunoassay analyzer comprises the following steps,

(S01) when the worker needs to detect the reagent, the worker can place the reagent tube in the connecting groove and start the second motor, the second motor drives the driving roller to rotate, the driving belt can be driven to rotate together through the rotation of the driving roller, and the driving belt is contacted with the bottom of the connecting groove, so that the reagent tube can be driven to move horizontally;

(S02) when the reagent tube is moved to the position under the liquid discharge tube by the conveying assembly, the sample and the reagent in the reagent box and the liquid storage box can be guided into the shell through the liquid guide tube, the sample and the reagent can collide with the fan blades in the process of guiding the reagent into the shell, so that the fan blades can perform autorotation work, and through arranging the through holes and the fixing dishes on one sides of the fan blades, the collision depth of the mixed reagent and the fan blades can be effectively increased, the stress effect of the fan blades is further improved, the fan blades can better rotate to perform mixing work on the sample and the reagent, and the subsequent detection error is further reduced;

(S03) when the reagent tube is conveyed to one end of the connecting groove, the reagent tube can be accurately moved to the position under the material pushing assembly through the two groups of guide plates and guide columns, when the reagent tube is moved to the position under the sealing cover through the conveying assembly, the first motor and the electric push rod are simultaneously started, the first motor drives the threaded lead screw to rotate, the threaded sleeve and the sealing cover can be driven to move to the position over the reagent tube through the threaded lead screw, then the electric push rod drives the sealing cover to descend until the sealing cover is clamped with the reagent tube, and at the moment, the first motor and the electric push rod are started again to move the reagent tube to the inner part of the sleeve, so that the full-automatic feeding work of the reagent tube is realized;

(S04) in the process of analyzing and detecting a sample by the device, the reagent tube is transferred into the sleeve through the conveying assembly and the pushing assembly until the bottom of the reagent tube is contacted with the friction gasket, at the moment, a worker starts the third motor which drives the second gear disc to rotate, the second gear disc is meshed with the first gear disc to drive the rotating rod to rotate, the connecting disc can rotate in the rotating process of the rotating rod, and the second tooth block arranged on the circumferential outer wall of the connecting disc is meshed with the first tooth block on the outer wall of the sleeve, so that the sleeve can rotate in the placing groove in the rotating process of the connecting disc, the reagent in the reagent tube can be fully mixed through the rotation of the sleeve, the accuracy of the subsequent detection result is improved, and the effect of revolution mixing around the connecting disc is better than the traditional method through the rotation of the sleeve, and the situation that the reagent is spilled out from the inside of the reagent tube due to overlarge centrifugal force can occur when the sleeve tube is subjected to revolution mixing;

(S05) at the in-process that the sleeve pipe carries out the rotation, because the bottom of reagent pipe contacts with the top of friction gasket, consequently, can play certain deceleration effect to the sleeve pipe through the friction gasket, avoid the sleeve pipe because rotate the condition that leads to reagent to spill from the reagent intraduct to take place at the excessive speed, and reagent pipe pivoted in-process, its bottom can contact and produce the heat with the friction gasket, through thereby can give a good greenhouse environment of reagent, make that reagent can be better react, because the commentaries on classics power that every reagent pipe received is all the same, consequently, can guarantee that the heat that every reagent pipe received is all the same, greatly reduced follow-up error to the sample detection.

The invention has the following beneficial effects:

(1) when the reagent tube is transferred into the sleeve through the conveying assembly and the pushing assembly until the bottom of the reagent tube is contacted with the friction gasket, the staff starts the third motor, the third motor drives the second gear disc to rotate, the second gear disc is meshed with the first gear disc in the process to drive the rotating rod to rotate, the connecting disc can be rotated in the rotating process of the rotating rod, and the second tooth block arranged on the circumferential outer wall of the connecting disc is meshed with the first tooth block on the outer wall of the sleeve, so that the sleeve can rotate in the placing groove in the rotating process of the connecting disc, and reagents in the reagent tube can be fully mixed through the rotation of the sleeve, so that the accuracy of subsequent detection results is improved;

(2) the effect of revolution mixing by the rotation of the sleeve is better than that of the traditional method of revolution mixing around the connecting disc, and the condition that the reagent is spilled out from the inside of the reagent tube can be led out due to overlarge centrifugal force when the sleeve is subjected to revolution mixing;

(3) in the process of autorotation of the sleeve, the bottom of the reagent tube is in contact with the top of the friction gasket, so that a certain speed reduction effect can be achieved on the sleeve through the friction gasket, the phenomenon that the reagent spills out of the reagent tube due to over-fast rotation of the sleeve is avoided, in addition, in the process of rotation of the reagent tube, the bottom of the reagent tube can be in contact with the friction gasket to generate heat, a good greenhouse environment can be provided for the reagent, the reagent can better react, and because the rotation force applied to each reagent tube is the same, the heat applied to each reagent tube can be ensured to be the same, and the error of subsequent sample detection is greatly reduced.

Drawings

FIG. 1 is a schematic view of the internal structure of the case of the present invention;

FIG. 2 is a schematic overall front view of the present invention;

FIG. 3 is an enlarged schematic view of FIG. 1 at A according to the present invention;

FIG. 4 is a schematic view of the overall backside structure of the present invention;

FIG. 5 is a schematic diagram of a disassembled structure of the box body of the present invention;

FIG. 6 is an enlarged view of the structure at B in FIG. 5 according to the present invention;

FIG. 7 is a schematic bottom view of the interface disc of the present invention;

FIG. 8 is a schematic view of the split structure of the bushing of the present invention;

fig. 9 is a schematic diagram of a hybrid module according to the present invention in a disassembled configuration.

The labels in the figures are: 100-a box body; 200-a connecting ring; 201-placing grooves; 202-a cannula; 203-a first tooth block; 204-a second tooth block; 205-connecting disc; 206-a first gear wheel; 207-rotating rods; 208-a third motor; 209-a positioning frame; 210-a positioning groove; 211-rubbing pads; 300-a photomultiplier tube; 400-a separator; 401-a transverse plate; 402-a first through slot; 403-vertical plate; 404-a first motor; 405-a threaded screw; 406-a slider; 407-a chute; 408-a threaded sleeve; 409-an electric push rod; 410-a sealing cover; 500-sealing the cover; 501-a liquid storage tank; 502-a connecting trough; 503-driving roller; 504-a drive belt; 505-a mounting plate; 506-a second motor; 507-a fixing frame; 508-a second through slot; 509-drain pipe; 510-a catheter; 511-guide post; 512-a guide plate; 513-a reinforcing frame; 514-reagent box; 515-a housing; 516-a via; 517-a fixed vessel; 518-rotating the column; 519-fan blades; 600-a control panel; 700-reagent tube.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention.

The components of embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention.

All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The full-automatic chemiluminescence immunoassay analyzer shown in fig. 1 and 2 comprises a box body 100, wherein a control panel 600 is fixedly connected to the outer wall of one side of the box body 100, photomultiplier tubes 300 are fixedly connected to the inner walls of two sides of the box body 100, reagent tubes 700 shown in fig. 4 are arranged on one side of the box body 100 in an equidistant distribution manner, a conveying assembly for driving the reagent tubes 700 to move horizontally is fixedly connected to one side of the box body 100, a material pushing assembly for feeding the reagent tubes 700 is arranged at the top of the conveying assembly, a positioning frame 209 is fixedly connected to the inner wall of the bottom of the box body 100, a third motor 208 is fixedly connected to the inner wall of the circumference of the positioning frame 209, a second gear disc is fixedly connected to one end of an output shaft of the third motor 208, a first gear disc 206 is engaged to the outer wall of the circumference of the second gear disc, a rotating rod 207 is fixedly connected to the inner wall of the circumference of the first gear disc 206, and a connecting disc 205 shown in fig. 7 is fixedly connected to one end of the rotating rod 207, the second tooth blocks 204 which are distributed in a circular shape at equal intervals are fixedly connected to the outer circumferential wall of the connecting disc 205, the connecting ring 200 is arranged on the outer circumferential wall of the connecting disc 205, the circle centers of the connecting ring 200 and the connecting disc 205 are the same, the outer top wall of the connecting ring 200 is provided with the placing grooves 201 which are distributed in a circular shape at equal intervals, the inner bottom wall of each placing groove 201 is provided with a positioning groove 210, the inner circumferential wall of each positioning groove 210 is connected with a sleeve 202 in a clamping manner, the outer circumferential wall of each sleeve 202 is fixedly connected with a first tooth block 203 shown in FIG. 8, the first tooth blocks 203 are mutually meshed with the second tooth blocks 204, the reagent tube 700 is inserted into the sleeve 202, the inner bottom wall of each positioning groove 210 is fixedly connected with a friction gasket 211, the inner bottom wall of the reagent tube 700 is contacted with the friction gasket 211, when the reagent tube 700 is transferred into the sleeve 202 through the conveying assembly and the material pushing assembly until the bottom of the reagent tube 700 is contacted with the friction gasket 211, at this time, the worker starts the third motor 208, the third motor 208 drives the second gear disc to rotate, in the process, the second gear disc is meshed with the first gear disc 206 to drive the rotating rod 207 to rotate, in the process, the connecting disc 205 can be rotated, as the second tooth blocks 204 arranged on the circumferential outer wall of the connecting disc 205 are meshed with the first tooth blocks 203 on the outer wall of the sleeve 202, the sleeve 202 can rotate in the placing groove 201 in the process of rotating the connecting disc 205, reagents in the reagent tube 700 can be fully mixed through the rotation of the sleeve 202, the accuracy of subsequent detection results is improved, meanwhile, the rotation through the sleeve 202 has a better effect than the traditional revolution mixing around the connecting disc 205, and in the process of revolution mixing of the sleeve 202, the reagent can be led out from the inside of the reagent tube 700 due to overlarge centrifugal force, in the process of autorotation of the sleeve 202, the bottom of the reagent tube 700 is in contact with the top of the friction gasket 211, so that a certain deceleration effect can be achieved on the sleeve 202 through the friction gasket 211, the situation that the reagent spills out of the reagent tube 700 due to too fast rotation of the sleeve 202 is avoided, in the process of rotation of the reagent tube 700, the bottom of the reagent tube can be in contact with the friction gasket 211 to generate heat, a good greenhouse environment can be provided for the reagent, the reagent can better react, and because the rotation force applied to each reagent tube 700 is the same, the heat applied to each reagent tube 700 can be ensured to be the same, and the error of subsequent sample detection is greatly reduced;

the conveying component comprises a sealing cover 500 fixedly connected to the outer wall of one side of the box body 100 and shown in fig. 5, a fixing frame 507 is fixedly connected to the outer wall of one side of the sealing cover 500, a mounting plate 505 is fixedly connected to one end, away from the sealing cover 500, of the fixing frame 507, a second motor 506 is fixedly connected to the outer wall of one side of the mounting plate 505, a driving roller 503 is fixedly connected to one end of an output shaft of the second motor 506, a driving belt 504 is connected to the circumferential outer wall of the driving roller 503 in a driving manner, an opening is formed in the outer wall of one side of the box body 100, a connecting groove 502 is fixedly connected to the inner wall of the bottom of the opening, the outer wall of the bottom of the driving belt 504 is in contact with the inner wall of the bottom of the connecting groove 502, the reagent tubes 700 are distributed at equal intervals at the top of the driving belt 504, a liquid adding component is arranged at the top of the sealing cover 500, guide components are arranged on the inner walls of two sides of the connecting groove 502, a reinforcing frame 513 shown in fig. 6 is fixedly connected to the outer wall of one side of the other mounting plate 505, one side fixed connection of strengthening frame 513 is on one side outer wall of spread groove 502, when the staff need examine reagent, the staff can place reagent pipe 700 inside spread groove 502 and start second motor 506, and second motor 506 drives driving roller 503 and rotates, can drive driving belt 504 together to rotate through the rotation of driving roller 503, and driving belt 504 contacts with the bottom of spread groove 502, consequently can drive reagent pipe 700 and do horizontal motion.

The liquid adding assembly comprises a liquid storage tank 501 and a reagent tank 514 which are fixedly connected to the outer wall of the top of the sealing cover 500, liquid guide pipes 510 are inserted into the bottoms of the liquid storage tank 501 and the reagent tank 514, the cross sections of the liquid guide pipes 510 are broken lines, a shell 515 shown in figure 9 is inserted into one end of each liquid guide pipe 510, a liquid discharge pipe 509 is inserted into the bottom of the shell 515, the liquid discharge pipe 509 is located right above the reagent pipe 700, the cross section of the shell 515 is spherical, a mixing assembly is arranged inside the shell 515, and when the reagent pipe 700 is moved to the position right below the liquid discharge pipe 509 by the conveying assembly, samples and reagents inside the reagent tank 514 and the liquid storage tank 501 can be guided into the shell 515 through the liquid guide pipes 510.

Mix the subassembly including rotating the rotation post 518 of connection at casing 515 circumference inner wall, the circumference outer wall fixedly connected with equidistance that rotates post 518 is circular distribution's flabellum 519, through-hole 516 has been seted up to one side of flabellum 519, the fixed ware 517 of one side outer wall fixedly connected with of through-hole 516, fixed ware 517 is the arc structure, can take place the striking with flabellum 519 at the inside in-process of the leading-in casing 515 of reagent, make flabellum 519 carry out rotation work, and set up through-hole 516 and set up fixed ware 517 in one side of flabellum 519, can effectual increase mix the impact depth degree of reagent and flabellum 519, further improved the atress effect of flabellum 519, make rotation that flabellum 519 can be better carry out mixed work to sample and reagent, further reduced subsequent detection error.

The guide assembly comprises guide plates 512 fixedly connected to the inner walls of the two sides of the connecting groove 502, the two groups of guide plates 512 are distributed on the two sides of the reagent tube 700 at equal intervals, the inner walls of the two sides of the guide plates 512 are rotatably connected with guide columns 511, the outer circumferential wall of each guide column 511 is in contact with the outer circumferential wall of the reagent tube 700, and when the reagent tube 700 is conveyed to one end of the connecting groove 502, the reagent tube 700 can accurately move to the position right below the pushing assembly through the two groups of guide plates 512 and the guide columns 511.

The material pushing assembly comprises a partition plate 400 fixedly connected to the inner walls of the two sides of the box body 100, a first through groove 402 is formed in the outer wall of one side of the partition plate 400, a transverse plate 401 is fixedly connected to the inner wall of the top of the first through groove 402, a driving assembly is fixedly connected to the outer wall of the bottom of the transverse plate 401, a limiting assembly is arranged at the bottom of the transverse plate 401, and the limiting assembly and the driving assembly are fixedly connected.

The driving component comprises a vertical plate 403 fixedly connected to the outer wall of the bottom of the transverse plate 401, a first motor 404 is fixedly connected to the outer wall of one side of the vertical plate 403, a threaded lead screw 405 is fixedly connected to one end of an output shaft of the first motor 404, a threaded sleeve 408 is meshed with the outer wall of the circumference of the threaded lead screw 405, an electric push rod 409 is fixedly connected to the outer wall of the bottom of the threaded sleeve 408, a sealing cover 410 is fixedly connected to the output end of the electric push rod 409, the specification of the sealing cover 410 is matched with that of the reagent tube 700, the first motor 404 and the electric push rod 409 are simultaneously started when the reagent tube 700 moves to the position right below the sealing cover 410 through the conveying component, the first motor 404 drives the threaded lead screw 405 to rotate, the threaded sleeve 408 and the sealing cover 410 can be driven to move to the position right above the reagent tube 700 through the threaded lead screw 405, and then the electric push rod drives the sealing cover 410 to descend until the sealing cover is clamped with the reagent tube 700, at this time, the first motor 404 and the electric push rod 409 are started again to move the reagent tube 700 to the inside of the sleeve 202, so that the full-automatic feeding work of the reagent tube 700 is realized.

Spacing subassembly is including offering the spout 407 at diaphragm 401 bottom outer wall, the equal sliding connection of both sides inner wall of spout 407 has slider 406, the bottom outer wall fixedly connected with down tube of slider 406, the down tube is kept away from and is formed fixed connection between the one end of slider 406 and threaded sleeve 408, when threaded sleeve 408 rotates along with the rotation of threaded lead screw 405, slide in spout 407 inside through slider 406, can play good limiting displacement to threaded sleeve 408, make threaded sleeve 408 can be stable do the horizontal motion.

A second through groove 508 is formed in one side of the sealing cover 500, a third through groove is formed in the bottom of the box body 100, the connecting groove 502 penetrates through the second through groove 508, and the transmission belt 504 penetrates through the third through groove.

A method for using a full-automatic chemiluminescence immunoassay analyzer comprises the following steps,

(S01) when the worker needs to test the reagent, the worker can place the reagent tube 700 in the connecting groove 502 and start the second motor 506, the second motor 506 drives the driving roller 503 to rotate, the driving belt 504 can be driven to rotate together by the rotation of the driving roller 503, and the driving belt 504 is in contact with the bottom of the connecting groove 502, so that the reagent tube 700 can be driven to move horizontally;

(S02) when the conveying assembly moves the reagent tube 700 to the position under the liquid discharge tube 509, the sample and the reagent in the reagent box 514 and the liquid storage box 501 can be guided into the shell 515 through the liquid guide tube 510, the sample and the reagent can collide with the fan blade 519 in the process of guiding the reagent into the shell 515, so that the fan blade 519 performs autorotation work, and through arranging the through hole 516 and the fixing dish 517 on one side of the fan blade 519, the collision depth of the mixed reagent and the fan blade 519 can be effectively increased, the stress effect of the fan blade 519 is further improved, the fan blade 519 can rotate better to perform mixing work on the sample and the reagent, and the subsequent detection error is further reduced;

(S03) when the reagent tube 700 is conveyed to one end of the connecting groove 502, the reagent tube 700 can be accurately moved to the position right below the material pushing component through the two sets of guide plates 512 and guide posts 511, when the reagent tube 700 is moved to the position right below the sealing cover 410 through the conveying component, the first motor 404 and the electric push rod 409 are simultaneously started, the first motor 404 drives the threaded screw rod 405 to rotate, the threaded sleeve 408 and the sealing cover 410 can be driven to move to the position right above the reagent tube 700 through the threaded screw rod 405, then the electric push rod 409 drives the sealing cover 410 to descend until the sealing cover is clamped with the reagent tube 700, at the moment, the first motor 404 and the electric push rod 409 are started again to move the reagent tube 700 to the inside of the sleeve 202, and therefore full-automatic feeding work of the reagent tube 700 is achieved;

(S04) the reagent tube 700 is transferred into the sleeve 202 through the conveying component and the pushing component until the bottom of the reagent tube 700 contacts with the friction pad 211, at this time, the staff starts the third motor 208, the third motor 208 drives the second gear disc to rotate, in the process, the second gear disc is meshed with the first gear disc 206 to drive the rotating rod 207 to rotate, in the rotating process of the rotating rod 207, the connecting disc 205 can be rotated, because the second gear block 204 arranged on the outer wall of the circumference of the connecting disc 205 is meshed with the first gear block 203 on the outer wall of the sleeve 202, in the rotating process of the connecting disc 205, the sleeve 202 can rotate in the placing groove 201, the reagent in the reagent tube 700 can be fully mixed through the rotation of the sleeve 202, the accuracy of the subsequent detection result is improved, and the effect of the revolution mixing performed around the connecting disc 205 through the rotation of the sleeve 202 is better than the conventional method of performing the revolution mixing around the connecting disc 205, and when the sleeve 202 performs revolution mixing, the reagent may be spilled from the inside of the reagent tube 700 due to excessive centrifugal force;

(S05) in the process of sleeve 202 rotation, because the bottom of reagent pipe 700 contacts with the top of friction gasket 211, therefore can play certain deceleration effect to sleeve 202 through friction gasket 211, avoid sleeve 202 because rotate the condition that causes reagent to spill from reagent pipe 700 inside too fast to take place, and reagent pipe 700 pivoted in-process, its bottom can contact with friction gasket 211 and produce the heat, through thereby can give reagent a good greenhouse environment, make reagent can better react, because the rotation force that each reagent pipe 700 received is all the same, consequently can guarantee that the heat that each reagent pipe 700 received is all the same, the follow-up error to the sample detection that has greatly reduced.

According to the invention, through the connecting ring 200, the sleeve 202, the connecting disc 205, the first tooth block 203 and the second tooth block 204, in the process of analyzing and detecting a sample by the device, the reagent tube 700 is transferred into the sleeve through the conveying assembly and the pushing assembly until the bottom of the reagent tube is contacted with the friction gasket 211, at this time, a worker starts the third motor 208, the third motor drives the second gear disc to rotate, the second gear disc is meshed with the first gear disc 206 to drive the rotating rod 207 to rotate in the process, the connecting disc can rotate in the rotating process of the rotating rod, as the second tooth block arranged on the circumferential outer wall of the connecting disc is meshed with the first tooth block on the outer wall of the sleeve, the sleeve can rotate in the placing groove 201 in the rotating process of the connecting disc, and reagents in the reagent tube can be fully mixed through the self-rotation of the sleeve, the accuracy of subsequent detection results is improved, the effect of revolution mixing around the connecting disc is better than that of the traditional method by the rotation of the sleeve, and when the sleeve is subjected to revolution mixing, the reagent is led out from the inside of the reagent tube possibly due to overlarge centrifugal force;

in-process that carries out the rotation at sleeve pipe 202, because the bottom of reagent pipe 700 contacts with the top of friction gasket 211, consequently, can play certain deceleration effect to the sleeve pipe through the friction gasket, avoid the sleeve pipe because rotate the condition that leads to reagent to spill from the reagent intraduct to take place at the excessive speed, and reagent pipe pivoted in-process, its bottom can contact and produce the heat with the friction gasket, through thereby can give a good greenhouse environment of reagent, make that reagent can be better react, because the commentaries on classics power homogeneous phase that every reagent pipe received is the same, consequently, can guarantee that the heat homogeneous phase that every reagent pipe received is the same, greatly reduced follow-up error to the sample detection.

According to the reagent tube feeding device, due to the arrangement of the conveying assembly and the guide assembly, when a worker needs to detect a reagent, the worker can place the reagent tube 700 in the connecting groove 502 and start the second motor 506, the second motor drives the driving roller 503 to rotate, the driving belt 504 can be driven to rotate together through the rotation of the driving roller, the driving belt is in contact with the bottom of the connecting groove, so that the reagent tube can be driven to move horizontally, when the reagent tube is conveyed to one end of the connecting groove, the reagent tube can be accurately moved to the position right below the material pushing assembly through the two groups of the guide plates 512 and the guide columns 511, the subsequent material pushing assembly can conveniently take the reagent tube, and the guide columns are of a circular structure, so that the reagent tube cannot be damaged in the process of guiding the reagent tube.

According to the invention, through the arranged material pushing assembly, when the reagent tube 700 moves to the position right below the sealing cover 410 through the conveying assembly, the first motor 404 and the electric push rod 409 are simultaneously started, the first motor drives the threaded screw rod 405 to rotate, the threaded screw rod can drive the threaded sleeve 408 and the sealing cover to move to the position right above the reagent tube, then the electric push rod drives the sealing cover to descend until the sealing cover is clamped with the reagent tube, and at the moment, the first motor and the electric push rod are started again to move the reagent tube to the interior of the sleeve, so that the full-automatic feeding work of the reagent tube is realized.

According to the invention, through the arrangement of the liquid adding assembly and the mixing assembly, when the reagent tube 700 is moved to the position right below the liquid discharge tube 509 by the conveying assembly, the sample and the reagent in the reagent box 514 and the liquid storage box 501 can be guided into the shell 515 through the liquid guide tube 510, and can collide with the fan blade 519 in the process of guiding the reagent into the shell, so that the fan blade performs autorotation operation, and through the arrangement of the through hole 516 and the arrangement of the fixed dish 517 on one side of the fan blade, the collision depth of the mixed reagent and the fan blade can be effectively increased, the stress effect of the fan blade is further improved, the sample and the reagent can be mixed through better rotation of the fan blade, and the subsequent detection error is further reduced.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims (10)

1. The utility model provides a full-automatic chemiluminescence immunoassay appearance, includes box (100), its characterized in that: the device comprises a box body (100), a control panel (600) is fixedly connected to the outer wall of one side of the box body (100), photomultiplier tubes (300) are fixedly connected to the inner walls of the two sides of the box body (100), a plurality of reagent tubes (700) are arranged on one side of the box body (100), a conveying assembly used for driving the reagent tubes (700) to move horizontally is fixedly connected to one side of the box body (100), a material pushing assembly used for feeding the reagent tubes (700) is arranged at the top of the conveying assembly, a positioning frame (209) is fixedly connected to the inner wall of the bottom of the box body (100), a third motor (208) is fixedly connected to the inner wall of the circumference of the positioning frame (209), a second gear disc is fixedly connected to one end of an output shaft of the third motor (208), a first gear disc (206) is meshed to the outer wall of the circumference of the second gear disc, a rotating rod (207) is fixedly connected to the inner wall of the circumference of the first gear disc (206), one end of the rotating rod (207) is fixedly connected with a connecting disc (205), the circumference outer wall of the connecting disc (205) is fixedly connected with a plurality of second tooth blocks (204) which are circularly distributed, the circumference outer wall of the connecting disc (205) is provided with a connecting ring (200), the circle centers of the connecting ring (200) and the connecting disc (205) are the same, the top outer wall of the connecting ring (200) is provided with a plurality of placing grooves (201) which are circularly distributed, the bottom inner wall of each placing groove (201) is provided with a positioning groove (210), the circumference inner wall of each positioning groove (210) is connected with a sleeve (202), the circumference outer wall of each sleeve (202) is fixedly connected with a first tooth block (203), the first tooth blocks (203) are mutually meshed with the second tooth blocks (204), the reagent tube (700) is inserted into the sleeve (202), and the bottom inner wall of each positioning groove (210) is fixedly connected with a friction gasket (211), the inner wall of the bottom of the reagent tube (700) is in contact with the friction pad (211).

2. The full-automatic chemiluminescence immunoassay analyzer according to claim 1, wherein: the conveying assembly comprises a sealing cover (500) fixedly connected to the outer wall of one side of the box body (100), a fixing frame (507) is fixedly connected to the outer wall of one side of the sealing cover (500), one end, far away from the sealing cover (500), of the fixing frame (507) is fixedly connected with a mounting plate (505), one outer wall of one side of the mounting plate (505) is fixedly connected with a second motor (506), one end of an output shaft of the second motor (506) is fixedly connected with a transmission roller (503), the outer wall of the circumference of the transmission roller (503) is in transmission connection with a transmission belt (504), an opening is formed in the outer wall of one side of the box body (100), a connecting groove (502) is fixedly connected to the inner wall of the bottom of the opening, the outer wall of the bottom of the transmission belt (504) is in contact with the inner wall of the bottom of the connecting groove (502), and a liquid adding assembly is arranged at the top of the sealing cover (500), the both sides inner wall of spread groove (502) all is provided with direction subassembly, another one side outer wall fixedly connected with of mounting panel (505) strengthens frame (513), one side fixed connection of strengthening frame (513) is in on the one side outer wall of spread groove (502).

3. The full-automatic chemiluminescence immunoassay analyzer according to claim 2, wherein: the liquid feeding assembly comprises a liquid storage box (501) and a reagent box (514) which are fixedly connected to the outer wall of the top of the sealing cover (500), a liquid guide pipe (510) is inserted into the bottom of the liquid storage box (501) and the bottom of the reagent box (514), a shell (515) is inserted into one end of the liquid guide pipe (510), a liquid discharge pipe (509) is inserted into the bottom of the shell (515), the liquid discharge pipe (509) is located right above the reagent pipe (700), and a mixing assembly is arranged inside the shell (515).

4. The full-automatic chemiluminescence immunoassay analyzer according to claim 3, wherein: the hybrid module is including rotating the connection and being in rotation post (518) of casing (515) circumference inner wall, the circumference outer wall fixedly connected with equidistance of rotation post (518) is circular distribution flabellum (519), through-hole (516) have been seted up to one side of flabellum (519), one side outer wall fixedly connected with of through-hole (516) is fixed ware (517), fixed ware (517) are the arc structure.

5. The full-automatic chemiluminescence immunoassay analyzer according to claim 2, wherein: the direction subassembly includes fixed connection in deflector (512) of spread groove (502) both sides inner wall, two sets of deflector (512) equidistance distributes the both sides of reagent pipe (700), the both sides inner wall of deflector (512) all rotates and is connected with guide post (511), the circumference outer wall of guide post (511) with the circumference outer wall of reagent pipe (700) contacts.

6. The full-automatic chemiluminescence immunoassay analyzer according to claim 1, wherein: the material pushing assembly comprises a partition plate (400) fixedly connected to the inner walls of two sides of the box body (100), a first through groove (402) is formed in the outer wall of one side of the partition plate (400), a transverse plate (401) fixedly connected to the inner wall of the top of the first through groove (402), a driving assembly fixedly connected to the outer wall of the bottom of the transverse plate (401), a limiting assembly is arranged at the bottom of the transverse plate (401), and the limiting assembly is fixedly connected with the driving assembly.

7. The full-automatic chemiluminescence immunoassay analyzer according to claim 6, wherein: the drive assembly comprises a vertical plate (403) fixedly connected to the outer wall of the bottom of the transverse plate (401), a first motor (404) fixedly connected to the outer wall of one side of the vertical plate (403), a threaded screw rod (405) fixedly connected to one end of an output shaft of the first motor (404), a threaded sleeve (408) meshed to the outer wall of the circumference of the threaded screw rod (405), an electric push rod (409) fixedly connected to the outer wall of the bottom of the threaded sleeve (408), a sealing cover (410) fixedly connected to the output end of the electric push rod (409), and the specification of the sealing cover (410) is matched with the specification of the reagent tube (700).

8. The full-automatic chemiluminescence immunoassay analyzer according to claim 7, wherein: the limiting assembly comprises a sliding groove (407) formed in the outer wall of the bottom of the transverse plate (401), sliding blocks (406) are connected to the inner walls of the two sides of the sliding groove (407) in a sliding mode, an inclined rod is fixedly connected to the outer wall of the bottom of each sliding block (406), and one end, far away from the corresponding sliding block (406), of each inclined rod is fixedly connected with the corresponding threaded sleeve (408).

9. The full-automatic chemiluminescence immunoassay analyzer according to claim 2, wherein: a second through groove (508) is formed in one side of the sealing cover (500), a third through groove is formed in the bottom of the box body (100), the connecting groove (502) penetrates through the second through groove (508), and the transmission belt (504) penetrates through the third through groove.

10. A use method of a full-automatic chemiluminescence immunoassay analyzer is characterized by comprising the following steps: comprises the following steps of (a) carrying out,

(S01) when the worker needs to detect the reagent, the worker can place the reagent tube (700) in the connecting groove (502) and start the second motor (506), the second motor (506) drives the driving roller (503) to rotate, the driving roller (503) rotates to drive the driving belt (504) to rotate together, and the driving belt (504) is in contact with the bottom of the connecting groove (502), so that the reagent tube (700) can be driven to move horizontally;

(S02) when the conveying assembly moves the reagent tube (700) to the position under the liquid discharge tube (509), the sample and the reagent in the reagent box (514) and the liquid storage box (501) can be guided into the shell (515) through the liquid guide tube (510), the sample and the reagent can collide with the fan blade (519) in the process of guiding the reagent into the shell (515), so that the fan blade (519) can rotate, and the collision depth of the mixed reagent and the fan blade (519) can be effectively increased by arranging the through hole (516) and the fixed dish (517) on one side of the fan blade (519), the stress effect of the fan blade (519) is further improved, the sample and the reagent can be mixed through better rotation of the fan blade (519), and the subsequent detection error is further reduced;

(S03) when the reagent tube (700) is conveyed to one end of the connecting groove (502), the reagent tube (700) can be accurately moved to the right below the material pushing component through the two groups of guide plates (512) and guide columns (511), when the reagent tube (700) moves to the position right below the sealing cover (410) through the conveying component, the first motor (404) and the electric push rod (409) are started at the same time, the first motor (404) drives the threaded screw rod (405) to rotate, the threaded sleeve (408) and the sealing cover (410) can be driven to move to the position right above the reagent tube (700) through the threaded screw rod (405), then the electric push rod (409) drives the sealing cover (410) to descend until the sealing cover and the reagent tube (700) are clamped, at the moment, the first motor (404) and the electric push rod (409) are started again to move the reagent tube (700) to the interior of the sleeve (202), and therefore full-automatic feeding work of the reagent tube (700) is achieved;

(S04) in the process of analyzing and detecting a sample by the device, the reagent tube (700) is transferred into the sleeve (202) through the conveying assembly and the pushing assembly until the bottom of the reagent tube (700) is contacted with the friction gasket (211), at the moment, a worker starts the third motor (208), the third motor (208) drives the second gear disc to rotate, the second gear disc is meshed with the first gear disc (206) to drive the rotating rod (207) to rotate, the connecting disc (205) can be rotated in the rotating process of the rotating rod (207), as the second gear block (204) arranged on the circumferential outer wall of the connecting disc (205) is meshed with the first gear block (203) on the outer wall of the sleeve, the sleeve (202) can rotate in the placing groove (201) in the rotating process of the connecting disc (205), and reagents in the reagent tube (700) can be fully mixed through the rotation of the sleeve (202), the accuracy of subsequent detection results is improved, meanwhile, the effect of revolution mixing performed around the connecting disc (205) through the rotation of the sleeve (202) is better than that of the traditional method, and the situation that the reagent spills out from the inside of the reagent tube (700) due to overlarge centrifugal force can occur when the sleeve (202) performs revolution mixing;

(S05) in the process of rotation of the sleeve (202), because the bottom of the reagent tube (700) is in contact with the top of the friction gasket (211), the friction gasket (211) can play a certain speed reduction effect on the sleeve (202), the phenomenon that the reagent spills out of the reagent tube (700) due to the fact that the sleeve (202) rotates too fast is avoided, in the process of rotation of the reagent tube (700), the bottom of the reagent tube can be in contact with the friction gasket (211) to generate heat, a good greenhouse environment can be provided for the reagent through the reagent tube, the reagent can better react, the rotation force received by each reagent tube (700) is the same, the heat received by each reagent tube (700) can be guaranteed to be the same, and the subsequent error of sample detection is greatly reduced.

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