CN114291985A - Method for inhibiting release of endogenous nitrogen and phosphorus in bottom sediment - Google Patents
Method for inhibiting release of endogenous nitrogen and phosphorus in bottom sediment Download PDFInfo
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- CN114291985A CN114291985A CN202111495043.8A CN202111495043A CN114291985A CN 114291985 A CN114291985 A CN 114291985A CN 202111495043 A CN202111495043 A CN 202111495043A CN 114291985 A CN114291985 A CN 114291985A
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 114
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 title claims abstract description 60
- 229910052698 phosphorus Inorganic materials 0.000 title claims abstract description 60
- 239000011574 phosphorus Substances 0.000 title claims abstract description 60
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 57
- 239000013049 sediment Substances 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 28
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 16
- 230000000813 microbial effect Effects 0.000 claims abstract description 39
- 238000002360 preparation method Methods 0.000 claims abstract description 37
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 33
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 33
- 239000001301 oxygen Substances 0.000 claims abstract description 33
- 239000002245 particle Substances 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 229910052500 inorganic mineral Inorganic materials 0.000 claims abstract description 15
- 239000011159 matrix material Substances 0.000 claims abstract description 15
- 239000011707 mineral Substances 0.000 claims abstract description 15
- 239000002101 nanobubble Substances 0.000 claims abstract description 15
- 241000894006 Bacteria Species 0.000 claims abstract description 8
- 241001107128 Myriophyllum Species 0.000 claims abstract description 6
- 238000009825 accumulation Methods 0.000 claims abstract description 6
- 230000000593 degrading effect Effects 0.000 claims abstract description 5
- 244000063299 Bacillus subtilis Species 0.000 claims description 26
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 26
- 239000000758 substrate Substances 0.000 claims description 21
- 241001495394 Nitrosospira Species 0.000 claims description 11
- 241000589516 Pseudomonas Species 0.000 claims description 9
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 7
- 241000605159 Nitrobacter Species 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 241000168053 Pseudomonas denitrificans (nomen rejiciendum) Species 0.000 claims description 5
- 230000001546 nitrifying effect Effects 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 3
- 238000000227 grinding Methods 0.000 claims description 3
- 238000003306 harvesting Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 241000589970 Spirochaetales Species 0.000 claims description 2
- QYFRTHZXAGSYGT-UHFFFAOYSA-L hexaaluminum dipotassium dioxosilane oxygen(2-) difluoride hydrate Chemical compound O.[O--].[O--].[O--].[O--].[O--].[O--].[O--].[O--].[O--].[F-].[F-].[Al+3].[Al+3].[Al+3].[Al+3].[Al+3].[Al+3].[K+].[K+].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O QYFRTHZXAGSYGT-UHFFFAOYSA-L 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 6
- 239000000969 carrier Substances 0.000 abstract description 2
- 235000010755 mineral Nutrition 0.000 description 10
- 239000010802 sludge Substances 0.000 description 7
- YGANSGVIUGARFR-UHFFFAOYSA-N dipotassium dioxosilane oxo(oxoalumanyloxy)alumane oxygen(2-) Chemical compound [O--].[K+].[K+].O=[Si]=O.O=[Al]O[Al]=O YGANSGVIUGARFR-UHFFFAOYSA-N 0.000 description 5
- 229910052627 muscovite Inorganic materials 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 238000005273 aeration Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 229910021532 Calcite Inorganic materials 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- 229910000278 bentonite Inorganic materials 0.000 description 1
- 239000000440 bentonite Substances 0.000 description 1
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 239000011810 insulating material Substances 0.000 description 1
- 239000012774 insulation material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000013557 nattō Nutrition 0.000 description 1
- 230000033116 oxidation-reduction process Effects 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 229910052604 silicate mineral Inorganic materials 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a method for inhibiting release of endogenous nitrogen and phosphorus in bottom sediment, and belongs to the technical field of bottom sediment treatment. The method comprises the following steps: adding oxygen nano bubble modified mineral particles serving as an improved matrix into the bottom mud to be treated, and providing micro-nano level oxygen-containing bubbles into the overlying water; adding a microbial preparation one week after the improved matrix is put in, degrading and converting nitrogen and phosphorus in the sediment, and inhibiting the release of endogenous nitrogen and phosphorus; finally, submerged plant myriophyllum pratense is planted on the improved matrix, endogenous nitrogen and phosphorus are absorbed and converted, and accumulation of endogenous nitrogen and phosphorus is reduced. According to the application, a treatment method combining a microbial preparation, oxygen nano-bubble modified mineral particles and a planted submerged plant, namely the watermifoil is adopted, the oxygen nano-bubble modified mineral particles provide carriers and places for growth of microbes and watermifoil, phosphorus accumulating bacteria and nitrification-denitrification bacteria provided by the microbial preparation further inhibit release of nitrogen and phosphorus, and meanwhile watermifoil can reduce interference of water body disturbance on oxygen nano-bubbles.
Description
Technical Field
The invention belongs to the technical field of bottom sediment treatment, and particularly relates to a method for inhibiting release of endogenous nitrogen and phosphorus in bottom sediment.
Background
In recent years, most of lakes in China have serious overproof nutrient salts such as nitrogen and phosphorus, which influences the development of socioeconomic performance. Although China increases the external source input control of nutrient salt, the damaged water body is repaired, and the ecology is restored. However, the endogenous release of nitrogen and phosphorus is often ignored because of being difficult to suppress, so that the water body treatment cannot be expected, the sediment serving as a storage part of the endogenous nitrogen and phosphorus in the water body is often influenced by factors such as temperature, dissolved oxygen, oxidation-reduction potential and the like, the nitrogen and phosphorus are released to the water body, meanwhile, the release of the nitrogen and phosphorus is promoted by external disturbance and the decomposition effect of microorganisms in an anaerobic environment, and the suppression of the release of the endogenous nitrogen and phosphorus becomes an important part for treating the water body.
The physical method for inhibiting the release of nitrogen and phosphorus generally uses sediment covering and aeration technologies. Sediment covering is a repairing technology for isolating overlying water and sediment by utilizing a covering layer, the covering layer can prevent pollutants in sediment from diffusing to the overlying water, such as adding sand, zeolite, calcite, soil, bentonite and the like, but can only inhibit in a short term due to the fact that the physicochemical property of the sediment cannot be changed, and the most effective sediment aeration in the aeration technology can improve the dissolved oxygen concentration, but can cause a large amount of release of nitrogen and phosphorus in the sediment, so that the release cannot be effectively controlled for a long time. The oxygen nano bubble modified mineral particles (ONBMMs) are oxygen modified porous muscovite, are not only excellent heat insulation materials, but also can continuously provide micro and nano oxygen-containing bubbles in the overlying water, so that a large amount of nitrogen and phosphorus is not released, and the method is a good choice for improving the bottom environment.
Compared with a chemical method, the method has the characteristics of easy great influence on water bodies, cost saving in bioremediation technology, small environmental influence, good treatment and remediation effects, simple operation and the like, and the main means comprises microbial preparation throwing and submerged plant planting, most of the microbial preparations on the market mainly use bacillus subtilis to inhibit phosphorus release, the influence on nitrogen release is insufficient, the research on the microbial preparation capable of influencing the nitrogen and phosphorus release becomes necessary, and the submerged plant planting can reduce endogenous nitrogen and phosphorus, but takes effect slowly and can only be used as a follow-up protection means. Therefore, it is very important to find a method for inhibiting the release of nitrogen and phosphorus in the sediment for a long time.
Disclosure of Invention
In view of the above drawbacks, the present invention provides a method for suppressing release of endogenous nitrogen and phosphorus in sediment.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a method for inhibiting release of endogenous nitrogen and phosphorus in sediment comprises the following steps: adding oxygen nano bubble modified mineral particles serving as an improved matrix into the bottom mud to be treated, and providing micro-nano level oxygen-containing bubbles into the overlying water; adding a microbial preparation one week after the improved matrix is put in, degrading and converting nitrogen and phosphorus in the sediment, and inhibiting the release of endogenous nitrogen and phosphorus; finally, submerged plant myriophyllum pratense is planted on the improved matrix, endogenous nitrogen and phosphorus are absorbed and converted, and accumulation of endogenous nitrogen and phosphorus is reduced.
Furthermore, the oxygen nano bubble modified mineral particles (ONBMMs) are oxygen modified porous muscovite, so that the content of dissolved oxygen at the bottom can be increased for a long time, the aerobic environment is manufactured to inhibit the release of nitrogen and phosphorus, and the oxygen modified porous muscovite can also be used as a heat insulating material to effectively inhibit the release of nitrogen and phosphorus caused by the increase of temperature.
Further, the thickness of the modified substrate covered above the substrate sludge to be treated is not less than 3 cm.
Further, calculating the initial area of the improved substrate covered above the sediment according to the area of the sediment to be treated, wherein the ratio of the initial area to the area of the sediment to be treated is not lower than 0.1.
Further, the microbial preparation is added one week after the improved matrix is put in, and the addition amount of the microbial preparation every time is 0.1 percent of the total amount of the improved matrix; the microbial preparation was added again every 3 days for 2 weeks.
The microbial preparation in the application consists of different types of microbes, the water content of the microbial preparation is less than 10%, and the bacteria concentration is 10 per gram7-1010A CFU; the method comprises the following specific steps:
(a) bacillus subtilis and Bacillus natto, both of which need to be fermented, harvested, dried and ground into powder with an average particle size of 200 microns, wherein about 60-80% of the mixture has a particle size of 150-500 microns.
(b) The strains of the nitrosospira, the nitrobacter and the pseudomonas denitrificans 3 need to be fermented, harvested, dried and ground into powder with the average particle size of 200 microns, wherein about 60-80 percent of the mixture has the particle size of 150-500 microns.
Wherein the ratio of the total weight of the bacillus subtilis and the bacillus natto to the total weight of the nitrosospira, the nitrobacter and the denitrifying pseudomonas is 1: 10-10: 1.
The weight ratio of the bacillus subtilis to the bacillus natto is 1: 1; the weight ratio of the nitrosospira to the nitrifying bacillus to the denitrifying pseudomonas is 1: 2.
The method is characterized in that nitrosospirillum, nitrobacillus and pseudomonas denitrificans are adopted to carry out nitration-denitrification reaction to inhibit the release of endogenous nitrogen, and bacillus subtilis and bacillus natto are adopted to carry out phosphorus polymerization reaction to inhibit the release of endogenous phosphorus, so that the release of nitrogen and phosphorus in bottom sediment is inhibited.
Furthermore, the myriophyllum is spike myriophyllum, Eh of the sediment can be improved, pH of the sediment is reduced, endogenous nitrogen and phosphorus can be absorbed and converted, and accumulation of endogenous nitrogen and phosphorus is reduced. The planting interval of the spike-shaped myriophyllum is 30 cm.
Has the advantages that: compared with the prior art, the invention has the advantages that:
1) according to the treatment method, the microbial preparation and the oxygen nano-bubble modified mineral particles are combined, the oxygen nano-bubble modified mineral particles provide carriers and places for microbial growth, oxygen is provided at the same time, the microbial reaction is enhanced by the oxygen, and the release of nitrogen and phosphorus is further inhibited by the phosphorus accumulating bacteria and the nitrification-denitrification bacteria provided by the microbial preparation.
2) By adopting the treatment method of combining the oxygen nano-bubble modified mineral particles with the planted submerged plant watermifoil, silicate minerals can provide a large amount of trace elements to promote the growth of watermifoil, and watermifoil can reduce the interference of water disturbance on the oxygen nano-bubbles on the surfaces of the oxygen nano-bubble modified mineral particles.
Detailed Description
The invention is further described with reference to specific examples. These examples are intended to illustrate the invention and are not intended to limit the scope of the invention. Modifications or substitutions to methods, procedures, or conditions of the invention may be made without departing from the spirit and scope of the invention. In the following examples, unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.
The oxygen nanobubble-modified mineral particles, bacillus subtilis, bacillus natto, spirochete, nitrobacter, and pseudomonas denitrificans used in the following examples were all commercially available.
Example 1
Selecting a region with an area of 5m3Into which oxygen nanobubble-modified mineral particles (oxygen-modified porous muscovite) are put as a modified substrate, providing oxygen-containing bubbles of micro-and nano-scale into the overlying water; and the thickness of the modified substrate covered above the substrate sludge to be treated is 4cm, and the ratio of the initial area of the modified substrate covered above the substrate sludge to the area of the substrate sludge to be treated is 0.2.
Fermenting, harvesting and drying bacillus subtilis, bacillus natto, nitrosospira, nitrobacillus and pseudomonas denitrificans, and processing according to the bacillus subtilis budThe weight ratio of the bacillus and the natto bacillus is 1: 1; the weight ratio of the nitrosospira to the nitrifying bacillus to the denitrifying pseudomonas is 1: 2; grinding the total weight of the bacillus subtilis and the bacillus natto and the total weight of the nitrosospira, the nitrobacter and the denitrifying pseudomonas in a ratio of 1: 10 into mixture powder with the average particle size of 200 microns, and mixing to prepare a microbial preparation; keeping the water content of the microbial preparation less than 10 percent and the concentration of the bacteria in the microbial preparation to be 10 per gram7CFU。
Adding a microbial preparation one week after the improved matrix is put in, wherein the addition amount of the microbial preparation every time is 0.1% of the total amount of the improved matrix; the microbial preparation was added again every 3 days for 2 weeks. The microbial preparation is used for degrading and converting nitrogen and phosphorus in the sediment to inhibit the release of endogenous nitrogen and phosphorus.
After the microbial preparation is added, the submerged plant watermifoil is planted on the improved substrate, endogenous nitrogen and phosphorus are absorbed and converted at the planting interval of 30cm, and the accumulation of endogenous nitrogen and phosphorus is reduced.
Example 2
Selecting a region with the area of 8m3The sediment 2 to be treated is added with oxygen nano-bubble modified mineral particles (oxygen modified porous muscovite) as an improved substrate, and oxygen-containing bubbles at micro-nano level are provided in the overlying water; and the thickness of the modified substrate covered above the substrate sludge to be treated is 6cm, and the ratio of the initial area of the modified substrate covered above the substrate sludge to the area of the substrate sludge to be treated is 0.3.
Fermenting, harvesting and drying bacillus subtilis, bacillus natto, nitrosospira, nitrobacillus and denitrifying pseudomonas by weight ratio of 1: 1; the weight ratio of the nitrosospira to the nitrifying bacillus to the denitrifying pseudomonas is 1: 1: 2; the ratio of the total weight of the bacillus subtilis and the bacillus natto to the total weight of the nitrosospira, the nitrobacter and the denitrifying pseudomonas is 10: 1 grinding into mixture powder with the average particle size of 200 microns, and mixing to prepare a microbial preparation; keeping the water content of the microbial preparation less than 10 percent and the concentration of the bacteria in the microbial preparation to be 10 per gram10CFU。
Adding a microbial preparation one week after the improved matrix is put in, wherein the addition amount of the microbial preparation every time is 0.1% of the total amount of the improved matrix; the microbial preparation was added again every 3 days for 2 weeks. The microbial preparation is used for degrading and converting nitrogen and phosphorus in the sediment to inhibit the release of endogenous nitrogen and phosphorus.
After the microbial preparation is added, the submerged plant watermifoil is planted on the improved substrate, endogenous nitrogen and phosphorus are absorbed and converted at the planting interval of 30cm, and the accumulation of endogenous nitrogen and phosphorus is reduced.
The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Within the scope of the technical idea of the invention, many simple modifications can be made to the technical solution of the invention, including combinations of various technical features in any other suitable way, and these simple modifications and combinations should also be regarded as the disclosure of the invention, and all fall within the scope of the invention.
Claims (10)
1. A method for inhibiting release of endogenous nitrogen and phosphorus in bottom sediment is characterized by comprising the following steps: adding oxygen nano bubble modified mineral particles serving as an improved matrix into the bottom mud to be treated, and providing micro-nano level oxygen-containing bubbles into the overlying water; adding a microbial preparation one week after the improved matrix is put in, degrading and converting nitrogen and phosphorus in the sediment, and inhibiting the release of endogenous nitrogen and phosphorus; finally, submerged plant myriophyllum pratense is planted on the improved matrix, endogenous nitrogen and phosphorus are absorbed and converted, and accumulation of endogenous nitrogen and phosphorus is reduced.
2. The method for suppressing the release of endogenous nitrogen and phosphorus in the sediment as recited in claim 1, wherein the oxygen nanobubble modified mineral particles are oxygen modified porous muscovite mica.
3. The method for suppressing the release of endogenous nitrogen and phosphorus in the sediment as claimed in claim 1, wherein the thickness of the modified substrate covered above the sediment to be treated is not less than 3 cm.
4. The method for suppressing the release of endogenous nitrogen and phosphorus in the sediment as claimed in claim 1, wherein the initial area of the modified substrate covered above the sediment is calculated according to the area of the sediment to be treated, and the ratio of the initial area to the area of the sediment to be treated is not lower than 0.1.
5. The method for inhibiting release of endogenous nitrogen and phosphorus in the sediment according to claim 1, wherein the microbial preparation is added one week after the modified substrate is added, and the addition amount of the microbial preparation is 0.1% of the total amount of the modified substrate each time; the microbial preparation was added again every 3 days for 2 weeks.
6. The method for inhibiting release of endogenous nitrogen and phosphorus in bottom sediment according to claim 1, wherein the microbial preparation is prepared by fermenting, harvesting, drying and grinding bacillus subtilis, bacillus natto, nitrosospira, nitrobacter and pseudomonas denitrificans into mixture powder with an average particle size of 200 microns, and mixing; the water content of the microbial preparation is less than 10 percent, and the concentration of the bacteria is 10 per gram7-1010CFU。
7. The method for suppressing the release of endogenous nitrogen and phosphorus in sediment as claimed in claim 6, wherein the particle size of 60% -80% of the mixture powder in the microbial preparation is 150-500 μm.
8. The method for inhibiting release of endogenous nitrogen and phosphorus in sediment according to claim 6, wherein the ratio of the total weight of the bacillus subtilis and the bacillus natto to the total weight of the spirochete, the nitrifying bacillus and the denitrifying pseudomonas is 1: 10-10: 1.
9. The method for inhibiting release of endogenous nitrogen and phosphorus in sediment according to claim 6, wherein the weight ratio of the bacillus subtilis to the bacillus natto is 1: 1; the weight ratio of the nitrosospira to the nitrifying bacillus to the denitrifying pseudomonas is 1: 2.
10. The method for inhibiting release of endogenous nitrogen and phosphorus in sediment according to claim 1, wherein the watermifoil is watermifoil, and the watermifoil is planted at intervals of 30 cm.
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| CN202111495043.8A CN114291985A (en) | 2021-12-08 | 2021-12-08 | Method for inhibiting release of endogenous nitrogen and phosphorus in bottom sediment |
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| CN202111495043.8A CN114291985A (en) | 2021-12-08 | 2021-12-08 | Method for inhibiting release of endogenous nitrogen and phosphorus in bottom sediment |
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Cited By (1)
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