CN114272428A - Antibacterial hemostatic band-aid and preparation method thereof - Google Patents
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Abstract
The invention discloses an antibacterial hemostatic band-aid and a preparation method thereof. Step 1: mixing and homogenizing a chitosan solution, a polyvinyl alcohol solution and a polyvinyl pyrrolidone solution to obtain a solution A; performing microwave crosslinking on the hydrogel to obtain hydrogel A; step 2: adding the hydrogel A into the curcumin solution, and stirring to obtain the antibacterial hydrogel; and step 3: coating the middle section of the medical adhesive tape with antibacterial hydrogel; spraying a papain solution on the antibacterial hydrogel layer, and performing ultraviolet irradiation; and drying to obtain the antibacterial hemostatic band-aid. Has the advantages that: (1) obtaining the double-network hydrogel which takes polyvinyl alcohol/polyvinylpyrrolidone as a soft structure network and takes bacterial cellulose, chitosan and calcium ions as a hard structure network. (2) Papain and curcumin are introduced to increase wound healing and antibacterial property, and the papain is effectively anchored on the surface of a hydrogel network by using the curcumin as a cross-linking agent to synergistically increase the stability and activity of the substance.
Description
Technical Field
The invention relates to the technical field of band-aid, in particular to an antibacterial hemostatic band-aid and a preparation method thereof.
Background
The band-aid is a surgical medical product with a hemostatic function and is widely applied to daily life. The types of the band-aids on the market are various. In the common band-aid, the material used for the wound hemostasis part is mostly a sponge cushion layer, although the type avoids the secondary injury of the wound when being stripped, the band-aid is used for the wound, the adhesiveness is not added, and the wound hemostasis recovery is slow. In recent years, hydrogel band-aids have been widely studied, and have good biocompatibility and good adhesion to wounds; due to the softness and smoothness of the hydrogel, secondary damage to the wound can not be caused when the wound is stripped; meanwhile, due to the self-swelling property of the hydrogel, the hydrogel can absorb tissue fluid of a wound and accelerate the healing of the wound.
In the current hydrogel, the hemostatic materials used mainly comprise collagen powder, zeolite, starch, chitosan and the like. Wherein, the zeolite or starch can generate heat due to absorbing tissue fluid, so that the wound generates inflammation; the tissue adhesion of the collagen powder is low; on the other hand, chitosan has natural antibacterial properties, but when used in a gel-like material, it has high adhesiveness, and when used in an excessive amount, it causes secondary damage upon peeling, but when used in an insufficient amount, it has weak antibacterial properties and is insufficient for inhibiting inflammation. In addition, the air permeability of the hydrogel is one of the key points, and is low, so that when the wound is wrapped for a long time, water vapor cannot be dispersed to cause the breeding of anaerobic bacteria, and the wound is not favorable for recovery.
On the other hand, active substances are often used to increase the antibacterial and anti-inflammatory properties of the band-aid, but the addition of the antibacterial substances has the problems of unstable active components and faster volatilization.
In conclusion, the preparation of the antibacterial hemostatic adhesive bandage is of great significance in solving the problems.
Disclosure of Invention
The invention aims to provide an antibacterial hemostatic band-aid and a preparation method thereof, so as to solve the problems in the background technology.
In order to solve the technical problems, the invention provides the following technical scheme:
a preparation method of the antibacterial hemostatic band-aid comprises the following steps:
step 1: mixing and homogenizing a chitosan solution, a polyvinyl alcohol solution and a polyvinyl pyrrolidone solution to obtain a solution A; performing microwave crosslinking, washing and purifying to obtain hydrogel A;
step 2: adding the hydrogel A into the curcumin solution, stirring, and filtering to obtain the antibacterial hydrogel;
and step 3: coating the middle section of the medical adhesive tape with antibacterial hydrogel; spraying a papain solution on the antibacterial hydrogel layer, and performing ultraviolet irradiation; and drying to obtain the antibacterial hemostatic band-aid.
Preferably, in the step 1, the concentration of the chitosan solution is 16-20 wt%; the concentration of the polyvinyl alcohol solution is 18-24 wt%; the concentration of the polyvinylpyrrolidone solution is 36-40 wt%; the volume ratio of the three solutions is 2:2 (2.5-3).
Preferably, in step 1, the reaction conditions of the microwave crosslinking are as follows: the microwave power is 500-600W, and the temperature of the microwave is 75-80 ℃; the microwave time is 2-3 minutes.
Preferably, in the step 2, the curcumin solution is a curcumin-ethanol solution with the concentration of 0.1-0.15 wt%, and the stirring time is 40-60 minutes.
Preferably, in step 3, the preparation method of the papain solution comprises the following steps: papain was dissolved in phosphate buffer solution at pH 7 ± 0.2 at a concentration of 0.1 mmol/L.
Optimally, in the step 3, in the ultraviolet illumination process, the wavelength is 350-365 nm; the illumination time is 10-15 minutes; the drying temperature is 50-60 ℃, and the drying time is 1-2 hours.
Preferably, the method comprises the following steps:
step 1: mixing and homogenizing a chitosan solution, a polyvinyl alcohol solution and a polyvinyl pyrrolidone solution to obtain a solution A; adding a bacterial cellulose solution containing calcium chloride into the solution A, and uniformly mixing to obtain a solution B; microwave crosslinking is carried out; then placing the hydrogel in a sulfuric acid solution, stirring and thickening, cooling, washing and purifying to obtain hydrogel B;
step 2: adding the hydrogel B into the curcumin solution, stirring, and filtering to obtain the antibacterial hydrogel;
and step 3: coating the middle section of the medical adhesive tape with antibacterial hydrogel; spraying a papain solution on the antibacterial hydrogel layer, and performing ultraviolet irradiation; and drying to obtain the antibacterial hemostatic band-aid.
Preferably, the concentration of the bacterial cellulose solution is 15-20 g/L; the addition amount of the calcium chloride is 12-14 wt% of the addition amount of the bacterial cellulose.
In the technical scheme, the double-network hydrogel which is a hard-structure network and is formed by taking polyvinyl alcohol/polyvinylpyrrolidone as a soft-structure network and bacterial cellulose, chitosan and calcium ions is prepared by utilizing microwave crosslinking and acid catalysis crosslinking. The prepared hydrogel has excellent toughness, good air permeability, and proper adhesiveness and hemostatic property. And the papain is anchored by curcumin, so that the antibacterial property and the wound healing are synergistically increased.
The specific explanation is as follows:
(1) normally, the crosslinking of the hydrogel will contain a crosslinking agent or initiator, but the present scheme uses microwave method and acid catalysis method, and utilizes covalent crosslinking and coordination of metal ions; two structural networks are formed, and the stability and the elasticity of the hydrogel are effectively enhanced. Meanwhile, the solubility of the chitosan is effectively increased in the acid catalysis process, and the formation of a gel network is facilitated. Moreover, the bacterial cellulose has a porous structure, and the porosity and the liquid absorption of the formed network hydrogel effectively ensure the dispersion of water vapor and inhibit the damage of long-term water to the wound.
(2) Papain and curcumin are introduced in the scheme. The papain is effectively anchored on the surface of the hydrogel network by using curcumin as a cross-linking agent. Curcumin is a hydrophobic polyphenol compound, hydrogel is soaked in curcumin solution and absorbed, and weak hydrogen bonds can be formed between the hydrogel and curcumin due to the existence of amino and carbonyl in the hydrogel, so that curcumin is adsorbed on the surface of the gel, then under ultraviolet irradiation, papain also contains amino and carbonyl, the two groups generate free radical reaction of oxygen and nitrogen, and covalent crosslinking is formed between the two groups and an ortho-dihydroxy group in the curcumin, so that the papain is immobilized on the surface of the hydrogel by taking curcumin as a medium. The method can be operated at room temperature by utilizing photo-crosslinking, and the activity and the structure of the enzyme are protected. Meanwhile, curcumin enables the activity of papain to be better.
It should be noted that: compared with the method of directly and covalently connecting papain with hydrogel, curcumin is adsorbed firstly, and because curcumin has ultraviolet absorptivity, the damage of ultraviolet rays to the hydrogel structure can be effectively avoided, bond breakage is avoided, the stability of the hydrogel is improved, and the load uniformity and load capacity of the papain are increased. Meanwhile, the curcumin is always limited in use due to hydrophobicity, can be loaded on hydrogel, can slowly release active components of the curcumin and promote wound healing, is matched with papain for use, has a good effect, has synergistic performance and promotes wound healing. Compared with free enzyme, the papain is fixed, so that the deformation and inactivation of the papain are effectively inhibited, the molecular structure of the papain has rigidity and is not changed, the biological catalytic activity of the papain is increased, inflammation is reduced, and tissue repair is facilitated. In addition, as bacterial cellulose, it has high porosity and high hydrophilicity, making it possible to physically immobilize biomolecules by adsorption, protecting enzymes from external interfaces such as aggregation and proteolysis.
Compared with the prior art, the invention has the following beneficial effects: (1) the double-network hydrogel which takes polyvinyl alcohol/polyvinylpyrrolidone as a soft structure network and takes bacterial cellulose, chitosan and calcium ions as a hard structure network is prepared. The prepared hydrogel has excellent toughness, good air permeability, and proper adhesiveness and hemostatic property. (2) According to the scheme, papain and curcumin are introduced to increase wound healing and antibacterial property, and the curcumin is used as a cross-linking agent to effectively anchor the papain on the surface of a hydrogel network so as to synergistically increase the stability and activity of the substance.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
step 1: mixing and homogenizing a chitosan solution, a polyvinyl alcohol solution and a polyvinylpyrrolidone solution in a volume ratio of 2:2:2.8 to obtain a solution A; adding a bacterial cellulose solution containing calcium chloride into the solution A, and uniformly mixing to obtain a solution B; setting the microwave power to 600W and the temperature of the microwave to 80 ℃; microwave crosslinking for 2.5 minutes; then placing the hydrogel in 40% sulfuric acid solution, stirring, concentrating, thickening, cooling, washing and purifying to obtain hydrogel B;
step 2: adding the hydrogel B into the curcumin solution, stirring for 45 minutes, and filtering to obtain the antibacterial hydrogel;
and step 3: coating the middle section of the medical adhesive tape with antibacterial hydrogel; spraying papain solution on the antibacterial hydrogel layer, and setting the ultraviolet wavelength at 365nm for illumination for 10 minutes; drying at 55 deg.C for 1.5 hr to obtain the final product.
In the technical scheme, the concentration of the chitosan solution is 20 wt%; the concentration of the polyvinyl alcohol solution is 24 wt%; the concentration of the polyvinylpyrrolidone solution was 40%. The concentration of the bacterial cellulose solution is 18 g/L; the addition amount of the calcium chloride is 14 percent of the addition amount of the bacterial cellulose. The curcumin solution is a curcumin-ethanol solution with the concentration of 0.12%. The preparation method of the papain solution comprises the following steps: papain was dissolved in phosphate buffer solution at pH 7.1 at a concentration of 0.1 mmol/L.
Example 2:
step 1: mixing and homogenizing a chitosan solution, a polyvinyl alcohol solution and a polyvinylpyrrolidone solution in a volume ratio of 2:2:2.5 to obtain a solution A; adding a bacterial cellulose solution containing calcium chloride into the solution A, and uniformly mixing to obtain a solution B; setting the microwave power to be 500W and the temperature of the microwave to be 75 ℃; microwave crosslinking is carried out for 2 minutes; then placing the hydrogel in 40% sulfuric acid solution, stirring, concentrating, thickening, cooling, washing and purifying to obtain hydrogel B;
step 2: adding the hydrogel B into the curcumin solution, stirring for 40 minutes, and filtering to obtain the antibacterial hydrogel;
and step 3: coating the middle section of the medical adhesive tape with antibacterial hydrogel; spraying a papain solution on the antibacterial hydrogel layer, and setting the ultraviolet wavelength at 350nm for illumination for 10 minutes; drying at 50 deg.C for 1 hr to obtain the final product.
In the technical scheme, the concentration of the chitosan solution is 16 wt%; the concentration of the polyvinyl alcohol solution is 18 wt%; the concentration of the polyvinylpyrrolidone solution was 36%. The concentration of the bacterial cellulose solution is 15 g/L; the addition amount of the calcium chloride is 12 percent of the addition amount of the bacterial cellulose. The curcumin solution is a curcumin-ethanol solution with the concentration of 0.1 percent. The preparation method of the papain solution comprises the following steps: papain was dissolved in phosphate buffer solution at pH 6.9 at a concentration of 0.1 mmol/L.
Example 3:
step 1: mixing and homogenizing a chitosan solution, a polyvinyl alcohol solution and a polyvinylpyrrolidone solution in a volume ratio of 2:2:3 to obtain a solution A; adding a bacterial cellulose solution containing calcium chloride into the solution A, and uniformly mixing to obtain a solution B; setting the microwave power to 600W and the temperature of the microwave to 80 ℃; microwave crosslinking for 3 minutes; then placing the hydrogel in 40% sulfuric acid solution, stirring, concentrating, thickening, cooling, washing and purifying to obtain hydrogel B;
step 2: adding the hydrogel B into the curcumin solution, stirring for 60 minutes, and filtering to obtain the antibacterial hydrogel;
and step 3: coating the middle section of the medical adhesive tape with antibacterial hydrogel; spraying a papain solution on the antibacterial hydrogel layer, and setting the ultraviolet wavelength to be 365nm for illumination for 15 minutes; drying at 60 deg.C for 2 hr to obtain the final product.
In the technical scheme, the concentration of the chitosan solution is 20 wt%; the concentration of the polyvinyl alcohol solution is 24 wt%; the concentration of the polyvinylpyrrolidone solution was 40%. The concentration of the bacterial cellulose solution is 20 g/L; the addition amount of the calcium chloride is 14 percent of the addition amount of the bacterial cellulose. The curcumin solution is a curcumin-ethanol solution with the concentration of 0.15 percent. The preparation method of the papain solution comprises the following steps: papain was dissolved in phosphate buffered saline at pH 7 at a concentration of 0.1 mmol/L.
Example 4: no bacterial cellulose is added;
step 1: mixing and homogenizing a chitosan solution, a polyvinyl alcohol solution and a polyvinylpyrrolidone solution in a volume ratio of 2:2:2.8 to obtain a solution A; setting the microwave power to 600W and the temperature of the microwave to 80 ℃; microwave crosslinking for 2.5 minutes; washing and purifying to obtain hydrogel A;
step 2: adding the hydrogel A into the curcumin solution, stirring for 45 minutes, and filtering to obtain the antibacterial hydrogel;
and step 3: coating the middle section of the medical adhesive tape with antibacterial hydrogel; spraying papain solution on the antibacterial hydrogel layer, and setting the ultraviolet wavelength at 365nm for illumination for 10 minutes; drying at 55 deg.C for 1.5 hr to obtain the final product.
In the technical scheme, the concentration of the chitosan solution is 20 wt%; the concentration of the polyvinyl alcohol solution is 24 wt%; the concentration of the polyvinylpyrrolidone solution was 40%. The curcumin solution is a curcumin-ethanol solution with the concentration of 0.12%. The preparation method of the papain solution comprises the following steps: papain was dissolved in phosphate buffer solution at pH 7.1 at a concentration of 0.1 mmol/L.
Example 5: reducing the addition of copper polyvinylpyrrolidone;
step 1: mixing and homogenizing a chitosan solution, a polyvinyl alcohol solution and a polyvinylpyrrolidone solution in a volume ratio of 2:2:1 to obtain a solution A; adding a bacterial cellulose solution containing calcium chloride into the solution A, and uniformly mixing to obtain a solution B; setting the microwave power to 600W and the temperature of the microwave to 80 ℃; microwave crosslinking for 2.5 minutes; then placing the hydrogel in 40% sulfuric acid solution, stirring, concentrating, thickening, cooling, washing and purifying to obtain hydrogel B;
step 2: adding the hydrogel B into the curcumin solution, stirring for 45 minutes, and filtering to obtain the antibacterial hydrogel;
and step 3: coating the middle section of the medical adhesive tape with antibacterial hydrogel; spraying papain solution on the antibacterial hydrogel layer, and setting the ultraviolet wavelength at 365nm for illumination for 10 minutes; drying at 55 deg.C for 1.5 hr to obtain the final product.
In the technical scheme, the concentration of the chitosan solution is 20 wt%; the concentration of the polyvinyl alcohol solution is 24 wt%; the concentration of the polyvinylpyrrolidone solution was 40%. The concentration of the bacterial cellulose solution is 18 g/L; the addition amount of the calcium chloride is 14 percent of the addition amount of the bacterial cellulose. The curcumin solution is a curcumin-ethanol solution with the concentration of 0.12%. The preparation method of the papain solution comprises the following steps: papain was dissolved in phosphate buffer solution at pH 7.1 at a concentration of 0.1 mmol/L.
Example 6: no curcumin is used;
step 1: mixing and homogenizing a chitosan solution, a polyvinyl alcohol solution and a polyvinylpyrrolidone solution in a volume ratio of 2:2:2.8 to obtain a solution A; adding a bacterial cellulose solution containing calcium chloride into the solution A, and uniformly mixing to obtain a solution B; setting the microwave power to 600W and the temperature of the microwave to 80 ℃; microwave crosslinking for 2.5 minutes; then placing the hydrogel in 40% sulfuric acid solution, stirring, concentrating, thickening, cooling, washing and purifying to obtain hydrogel B;
step 2: coating hydrogel B on the middle section of the medical adhesive tape; spraying papain solution on the antibacterial hydrogel layer, and setting the ultraviolet wavelength at 365nm for illumination for 10 minutes; drying at 55 deg.C for 1.5 hr to obtain the final product.
In the technical scheme, the concentration of the chitosan solution is 20 wt%; the concentration of the polyvinyl alcohol solution is 24 wt%; the concentration of the polyvinylpyrrolidone solution was 40%. The concentration of the bacterial cellulose solution is 18 g/L; the addition amount of the calcium chloride is 14 percent of the addition amount of the bacterial cellulose. The preparation method of the papain solution comprises the following steps: papain was dissolved in phosphate buffer solution at pH 7.1 at a concentration of 0.1 mmol/L.
Example 7: papain is not used;
step 1: mixing and homogenizing a chitosan solution, a polyvinyl alcohol solution and a polyvinylpyrrolidone solution in a volume ratio of 2:2:2.8 to obtain a solution A; adding a bacterial cellulose solution containing calcium chloride into the solution A, and uniformly mixing to obtain a solution B; setting the microwave power to 600W and the temperature of the microwave to 80 ℃; microwave crosslinking for 2.5 minutes; then placing the hydrogel in 40% sulfuric acid solution, stirring, concentrating, thickening, cooling, washing and purifying to obtain hydrogel B;
step 2: adding the hydrogel B into the curcumin solution, stirring for 45 minutes, and filtering to obtain the antibacterial hydrogel;
and step 3: coating the middle section of the medical adhesive tape with antibacterial hydrogel; drying at 55 deg.C for 1.5 hr to obtain the final product.
In the technical scheme, the concentration of the chitosan solution is 20 wt%; the concentration of the polyvinyl alcohol solution is 24 wt%; the concentration of the polyvinylpyrrolidone solution was 40%. The concentration of the bacterial cellulose solution is 18 g/L; the addition amount of the calcium chloride is 14 percent of the addition amount of the bacterial cellulose. The curcumin solution is a curcumin-ethanol solution with the concentration of 0.12%.
Experiment: the antibacterial hemostatic band-aid prepared in examples 1 to 7 was tested as follows:
(1) the hydrogel on the woundplast is quantitatively smeared in the center of a filter paper sheet by adopting a paper sheet method diffusion method and is stuck to the agar surface of the staphylococcus aureus to be tested. And judging the antibacterial efficiency through the diameter of the antibacterial zone. (2) The 180 DEG peel force was tested at a speed of 300mm/min using an electronic peel tester. (3) SPF male SD rats purchased from Changzhou Kavens laboratory animals GmbH weighed 250-270 g. 21 rats were previously fed for 2 weeks under the same conditions and then subjected to the experiment. Pentobarbital sodium is injected into the abdominal cavity, and rumen injection is performed for anesthesia; under aseptic conditions, back hair was removed, a 1 × 8mm wound was created using a scalpel, the prepared wound patch was placed on the wound, the average hemostatic time was measured, and the surface, the degree of closure of the wound after day 5 was measured. The results obtained are all expressed as mean. + -. Standard Deviation (SD) of n > 3. Meanwhile, the difference of each group is analyzed by adopting single-factor variance, and the statistical significance is achieved when p is less than 0.05 and p is less than 0.01.
| Diameter of zone of inhibition | Mean time to hemostasis | Degree of wound closure | Peel strength | |
| Example 1 | 2.31cm | 7s | 58％ | 5.83N/25mm |
| Example 2 | 2.26cm | 10s | 56％ | 5.81N/25mm |
| Example 3 | 2.28cm | 8s | 57％ | 5.84N/25mm |
| Example 4 | 2.29cm | 8s | 55％ | 5.92N/25mm |
| Example 5 | 2.19cm | 11s | 52％ | 5.85N/25mm |
| Example 6 | 2.08cm | 14s | 50％ | 5.92N/25mm |
| Example 7 | 2.11cm | 12s | 51％ | 5.88N/25mm |
And (4) conclusion: as can be seen from the table: the band-aid prepared in the examples 1 to 3 has excellent antibacterial property, hemostatic property and wound healing promoting property. In example 4, the bacterial cellulose was not added, so that the rigidity of the hydrogel was reduced, and the hydrogel network was not uniform, so that the peel strength was increased, and there was damage to the wound during peeling. Meanwhile, due to the porosity and the hydrophilicity of the bacterial cellulose, the papain can be physically adsorbed, the activity of the papain is increased, and the wound closure degree is improved, so that the performance in the aspect is slightly reduced. The examples show that the performance is slightly reduced by reducing the addition amount of the polyvinylpyrrolidone, because the polyvinylpyrrolidone has amphiphilicity and can have higher affinity for curcumin of a hydrophobic polyphenol compound, and therefore, the hydrogel has a large amount of PVP (polyvinyl pyrrolidone), and can promote the adsorption of the curcumin, so that the loading of the papain is increased. Increasing the biological activity of the adhesive bandage. In examples 6 to 7, however, it was found that: if curcumin and papain lack one, the performance is reduced because both have good antibacterial activity and wound healing property. And the curcumin fixes the papain, thereby effectively inhibiting the deformation and inactivation of the protease. The rigidity of the molecular structure of the papain is increased, thereby enhancing the biological activity of the papain. The two have synergistic effect. At the same time, it can be found that: the papain is directly loaded on the hydrogel, so that the structure of the hydrogel is influenced, the loading capacity of the papain is reduced, and the antibacterial and hemostatic properties are influenced.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. A preparation method of an antibacterial hemostatic band-aid is characterized by comprising the following steps: the method comprises the following steps:
step 1: mixing and homogenizing a chitosan solution, a polyvinyl alcohol solution and a polyvinyl pyrrolidone solution to obtain a solution A; performing microwave crosslinking, washing and purifying to obtain hydrogel A;
step 2: adding the hydrogel A into the curcumin solution, stirring, and filtering to obtain the antibacterial hydrogel;
and step 3: coating the middle section of the medical adhesive tape with antibacterial hydrogel; spraying a papain solution on the antibacterial hydrogel layer, and performing ultraviolet irradiation; and drying to obtain the antibacterial hemostatic band-aid.
2. The preparation method of the antibacterial hemostatic band-aid according to claim 1, characterized in that: the method comprises the following steps: in the step 1, the concentration of the chitosan solution is 16-20 wt%; the concentration of the polyvinyl alcohol solution is 18-24 wt%; the concentration of the polyvinylpyrrolidone solution is 36-40 wt%; the volume ratio of the three solutions is 2:2 (2.5-3).
3. The preparation method of the antibacterial hemostatic band-aid according to claim 1, characterized in that: the method comprises the following steps: in the step 1, the reaction conditions of the microwave crosslinking are as follows: the microwave power is 500-600W, and the temperature of the microwave is 75-80 ℃; the microwave time is 2-3 minutes.
4. The preparation method of the antibacterial hemostatic band-aid according to claim 1, characterized in that: the method comprises the following steps: in the step 2, the curcumin solution is a curcumin-ethanol solution with the concentration of 0.1-0.15 wt%, and the stirring time is 40-60 minutes.
5. The preparation method of the antibacterial hemostatic band-aid according to claim 1, characterized in that: the method comprises the following steps: in step 3, the preparation method of the papain solution comprises the following steps: papain was dissolved in phosphate buffer solution at pH 7 ± 0.2 at a concentration of 0.1 mmol/L.
6. The preparation method of the antibacterial hemostatic band-aid according to claim 1, characterized in that: the method comprises the following steps: in the step 3, in the ultraviolet illumination process, the wavelength is 350-365 nm; the illumination time is 10-15 minutes; the drying temperature is 50-60 ℃, and the drying time is 1-2 hours.
7. The preparation method of the antibacterial hemostatic band-aid according to claim 1, characterized in that: the method comprises the following steps:
step 1: mixing and homogenizing a chitosan solution, a polyvinyl alcohol solution and a polyvinyl pyrrolidone solution to obtain a solution A; adding a bacterial cellulose solution containing calcium chloride into the solution A, and uniformly mixing to obtain a solution B; microwave crosslinking is carried out; then placing the hydrogel in a sulfuric acid solution, stirring and thickening, cooling, washing and purifying to obtain hydrogel B;
step 2: adding the hydrogel B into the curcumin solution, stirring, and filtering to obtain the antibacterial hydrogel;
and step 3: coating the middle section of the medical adhesive tape with antibacterial hydrogel; spraying a papain solution on the antibacterial hydrogel layer, and performing ultraviolet irradiation; and drying to obtain the antibacterial hemostatic band-aid.
8. The preparation method of the antibacterial hemostatic band-aid according to claim 7, characterized in that: the method comprises the following steps: the concentration of the bacterial cellulose solution is 15-20 g/L; the addition amount of the calcium chloride is 12-14 wt% of the addition amount of the bacterial cellulose.
9. The antibacterial hemostatic adhesive bandage prepared by the preparation method of the antibacterial hemostatic adhesive bandage according to any one of claims 1 to 8.
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