CN108484936B - Hydrogel prepared from graft modified material and preparation method and application thereof - Google Patents
Hydrogel prepared from graft modified material and preparation method and application thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 108010039918 Polylysine Proteins 0.000 claims abstract description 59
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- 238000012986 modification Methods 0.000 claims abstract description 6
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- BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 3
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- LDXJRKWFNNFDSA-UHFFFAOYSA-N 2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-1-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]ethanone Chemical compound C1CN(CC2=NNN=C21)CC(=O)N3CCN(CC3)C4=CN=C(N=C4)NCC5=CC(=CC=C5)OC(F)(F)F LDXJRKWFNNFDSA-UHFFFAOYSA-N 0.000 description 1
- YLZOPXRUQYQQID-UHFFFAOYSA-N 3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)-1-[4-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidin-5-yl]piperazin-1-yl]propan-1-one Chemical compound N1N=NC=2CN(CCC=21)CCC(=O)N1CCN(CC1)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F YLZOPXRUQYQQID-UHFFFAOYSA-N 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
- C08J3/075—Macromolecular gels
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0019—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F2/00—Processes of polymerisation
- C08F2/46—Polymerisation initiated by wave energy or particle radiation
- C08F2/48—Polymerisation initiated by wave energy or particle radiation by ultraviolet or visible light
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F283/00—Macromolecular compounds obtained by polymerising monomers on to polymers provided for in subclass C08G
- C08F283/04—Macromolecular compounds obtained by polymerising monomers on to polymers provided for in subclass C08G on to polycarbonamides, polyesteramides or polyimides
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2377/00—Characterised by the use of polyamides obtained by reactions forming a carboxylic amide link in the main chain; Derivatives of such polymers
- C08J2377/04—Polyamides derived from alpha-amino carboxylic acids
Abstract
The invention relates to a hydrogel prepared from a graft modification material, and a preparation method and application thereof. The hydrogel is formed by grafting polylysine to polyacrylamide and has an interpenetrating network structure. The hydrogel provided by the invention not only has stronger mechanical properties, proper wound adhesion and excellent antibacterial property, but also has higher saturated water absorption rate, and can be used for preparing wound repair products. The preparation method of the hydrogel provided by the invention has the advantages of simple process and high yield.
Description
Technical Field
The invention belongs to the technical field of medical materials, and particularly relates to hydrogel prepared from a grafting modified material, and a preparation method and application thereof.
Background
The hydrogel medical wound auxiliary material is a novel wound auxiliary material developed in recent years. Compared with the traditional auxiliary materials, the hydrogel can promote the wound to heal better, relieve the pain of patients, improve the microenvironment of the wound surface and inhibit the growth of bacteria.
In 1977, Japanese scholars extracted homomorphic monomer polymers containing 25-30 lysine residues from actinomycetes culture filtrate. This lysine polymer is formed by linking lysine residues via amide bonds formed by alpha-carboxyl groups and-amino groups, and is called polylysine. The application of polylysine as a natural polymer in the preparation of hydrogels has the following advantages: firstly, a molecular chain has a large number of amino active groups, the amino active groups are combined with positive charges on hydrogen ions under an aqueous solution or an acidic environment to form a cationic polymer, the cationic polymer can be well combined on the surface of cells so as to achieve the purpose of adhesion of wound tissues, secondly, polylysine is a natural high molecular material and has good biocompatibility, and a degradation product lysine is essential amino acid for a human body, and finally, polylysine is an amino acid polymer and has good water solubility, so that the difficulty that a plurality of tissue healing materials are difficult to dissolve in water is overcome. However, in the prior art, polylysine is used for preparing hydrogel, and the main method is irradiation crosslinking, chemical crosslinking or preparation of hydrogel by physical mixing. Hydrogels prepared by these techniques have the problem of poor mechanical properties, leading to fragility and reduced adhesion of the hydrogels, limiting their clinical applications.
Therefore, the development of the polylysine hydrogel with good mechanical properties has important research significance and application value.
Disclosure of Invention
The invention aims to overcome the defect and deficiency of the prior polylysine hydrogel medical material in poor mechanical property, and provides a hydrogel with strong mechanical property and suitable tissue adhesion. The hydrogel provided by the invention is the hydrogel with an interpenetrating network structure, which is obtained by grafting polyacrylamide on polylysine, combines the advantages of natural macromolecules and synthetic macromolecules, can retain the mechanical strength of the synthetic macromolecules, has good biocompatibility, suitable tissue adhesion and excellent antibacterial performance, and simultaneously improves the saturated water absorption rate of colloid.
Another object of the present invention is to provide a method for producing the above hydrogel.
The invention also aims to provide application of the hydrogel in preparation of chronic wound repair plugging products, diabetic foot repair products or surgical wound repair products.
In order to achieve the purpose, the invention adopts the following technical scheme:
the hydrogel is formed by grafting polylysine to polyacrylamide and has an interpenetrating network structure.
The invention provides the hydrogel with better mechanical property, which is obtained by grafting and modifying polylysine, wherein the hydrogel is grafted with polyacrylamide on the polylysine to form an interpenetrating cross-linked network, so that the mechanical property of the hydrogel is greatly improved, the hydrogel has good tissue adhesion, the saturated water absorption of the gel is improved, and the hydrogel has excellent antibacterial property.
Preferably, the grafting rate of the polylysine is 10 to 45 percent.
Preferably, the polylysine has a molecular weight of 3000-5000 Da. Polylysine has a molecular weight between 3000 and 5000 daltons, and is similar in protein composition and function to native extracellular matrix (ECM).
Preferably, the tensile elongation of the hydrogel is 1000-2500%, and the saturated water absorption is 400-800%.
Preferably, the tissue adhesion strength of the hydrogel is 1-3 Mpa, which is superior to that of the hydrogel prepared by polylysine alone.
The invention also provides a preparation method of the hydrogel, which comprises the following steps:
s1: adding 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide EDC and N-hydroxysuccinimide NHS into a polylysine aqueous solution, reacting for 4-8 h, dialyzing, and freeze-drying the dialyzed solution to obtain modified polylysine;
s2: dissolving the modified polylysine obtained in the step S1 in water, adding an acrylamide monomer, stirring, uniformly mixing, adding a photoinitiator ammonium persulfate and N, N' -methylene-bisacrylamide, continuously stirring, and then irradiating by ultraviolet light;
s3: and then adjusting the pH value to 8-10, and standing to obtain the hydrogel.
The hydrogel with strong mechanical property, high saturated water absorption and better wound healing promoting effect can be obtained by the preparation method of the hydrogel. The modified polylysine is obtained by activating the carboxyl of the polylysine, then the mixture comprising the polyacrylamide and the modified polylysine is obtained by photopolymerization, and finally the amino on the polyacrylamide is grafted to the carboxyl of the polylysine to form an interpenetrating cross-linked network structure, so that the mechanical strength and the saturated water absorption rate of the polylysine are greatly improved, and the polylysine has good antibacterial property. The preparation method of the hydrogel material provided by the invention has the advantages of simple process and high yield.
In the invention, polylysine can be dissolved in deionized water, purified water, ultrapure water and the like, and preferably, the mass concentration of the polylysine solution is 5-10%.
Preferably, the mass ratio of EDC to NHS in S1 is 1: 1-5, and the mass ratio of the sum of EDC and NHS to polylysine is 5: 100-10: 100; preferably, the mass ratio of EDC and NHS is 1: 1. Under the condition, the activation condition of the carboxyl on the polylysine is optimal, which is beneficial to improving the grafting rate and obtaining the hydrogel with stronger mechanical property.
Preferably, the mass concentration of the modified polylysine in S2 after being dissolved in water is 10-20%; the mass ratio of the modified polylysine to the acrylamide monomer is 1: 10-10: 1, and preferably 1: 1. The inventor of the invention discovers that the grafting rate can be influenced by controlling the mass ratio of the modified polylysine to the acrylamide monomer through multiple researches, and the higher the grafting rate is in a certain range, the stronger the mechanical capacity of the obtained interpenetrating network hydrogel is. And the saturated water absorption of the hydrogel gradually increases with the increase of the dosage of the used modified polylysine, and gradually decreases with the increase of the dosage of the modified polylysine after reaching the maximum value. The hydrogel contains partially ionized-COO on the molecular chain－and-NH3 +Containing both non-ionised-COOH and-NH2. On the one hand due to-COO－and-NH3 +The existence of the hydrogel increases the hydrophilicity, and water molecules are easier to diffuse into the gel, so that the water absorption performance of the gel is increased; on the other hand due to-COO－and-NH3 +Easy formation of internal salts, plus the presence of non-ionized-COOH and-NH2Gels ofHydrogen bonds are easily formed between molecular chains, and both the molecular chains play a role of physical cross-linking points; as the crosslinking density of the gel increases, the water absorption performance decreases.
Preferably, the mass ratio of ammonium persulfate to N, N' -methylene-bisacrylamide in S2 is 1: 1; the mass ratio of the sum of the mass of the ammonium persulfate and the mass of the N, N' -methylene-bisacrylamide to the mass of the acrylamide monomer is 1: 100-5: 100.
Preferably, the photoinitiator ammonium persulfate and the N, N' -methylene-bisacrylamide are added into S2, and the stirring is continued for 30 min-2 h.
Preferably, the wavelength of the ultraviolet light in S2 is 300-500 nm. More preferably, the wavelength of the ultraviolet light in S2 is 350-370 nm, and the irradiation time of the ultraviolet light is 8-12 h.
Preferably, the pH in S3 is adjusted to 9. This condition modified polylysine to the highest degree of ionization of the activated carboxyl groups.
Preferably, the standing time in S3 is 30 min.
The application of the hydrogel in preparing chronic wound repair plugging products, diabetic foot repair products or surgical wound repair products is also within the protection scope of the invention.
Compared with the prior art, the invention has the following beneficial effects:
the hydrogel prepared from the graft modification material provided by the invention is a polylysine-polyacrylamide graft gel polymer with an interpenetrating cross-linked network, which is obtained by grafting polyacrylamide on polylysine, has strong mechanical properties, good biocompatibility, suitable tissue adhesion and excellent antibacterial performance, improves the saturated water absorption rate of colloid, and can be used for preparing wound repair products. The preparation method of the material provided by the invention has the advantages of simple process and high yield.
Drawings
FIG. 1 shows the zone of inhibition.
Detailed Description
The invention is further illustrated by the following examples. These examples are intended to illustrate the invention and are not intended to limit the scope of the invention. Experimental procedures without specific conditions noted in the examples below, generally according to conditions conventional in the art or as suggested by the manufacturer; the raw materials, reagents and the like used are, unless otherwise specified, those commercially available from the conventional markets and the like. Any insubstantial changes and substitutions made by those skilled in the art based on the present invention are intended to be covered by the claims.
Example 1
This example provides a hydrogel that is prepared by the following method:
weighing 5g of polylysine (molecular weight is 3000-5000) into a container, adding 100ml of deionized water, stirring to dissolve, adding 0.125g of EDC and 0.125g of NHS, and reacting for 4 h. Taking out the solution, dialyzing, and freeze-drying the solution in the dialysis bag to obtain the modified polylysine. 2g of modified polylysine is weighed into a container, 10ml of deionized water is added, the mixture is stirred to be dissolved, 2g of acrylamide monomer is added into the container, and the mixture is stirred to be dissolved. 0.01g of ammonium persulfate photoinitiator and 0.01g of N, N' -methylenebisacrylamide were added to the vessel and stirring was continued for 30 min. The solution was poured into a vessel and irradiated overnight using a 365nm UV light generator. The pH was adjusted to 8 using a sodium borate buffer solution. Standing for 30min to obtain the polylysine-polyacrylamide grafted gel-like polymer with the grafting rate of 10%.
Example 2
This example provides a hydrogel prepared by the following method:
weighing 1.5g polylysine (molecular weight is 3000-5000) in a container, adding 60ml purified water, stirring to dissolve, adding 0.25g EDC and 1.25g NHS, and reacting for 8 h. Taking out the solution, dialyzing, and freeze-drying the solution in the dialysis bag to obtain the modified polylysine. 2g of modified polylysine is weighed into a container, 20ml of deionized water is added, the mixture is stirred and dissolved, 10g of acrylamide monomer is added into the container, and the mixture is stirred and dissolved. 0.05g of ammonium persulfate photoinitiator and 0.05g of N, N' -methylenebisacrylamide were added to the vessel and stirring was continued for 1 h. The solution was poured into a vessel and irradiated overnight using a 350nm UV light generator. The pH was adjusted to 10 using a sodium borate buffer solution. Standing for 30min to obtain the polylysine-polyacrylamide grafted gel-like polymer with the grafting rate of 45%.
Example 3
This example provides a hydrogel prepared by the following method:
5g of polylysine (molecular weight is 3000-5000) is weighed into a container, 50ml of ultrapure water is added for dissolution, 0.25g of EDC and 0.5g of NHS are added, and reaction is carried out for 6 h. Taking out the solution, dialyzing, and freeze-drying the solution in the dialysis bag to obtain the modified polylysine. 2g of modified polylysine is weighed into a container, 15ml of deionized water is added, the mixture is stirred to be dissolved, 0.5g of acrylamide monomer is added into the container, and the mixture is stirred to be dissolved. 0.025g of ammonium persulfate photoinitiator and 0.025g of N, N' -methylenebisacrylamide were added to the vessel and stirring was continued for 2 h. The solution was poured into a vessel and irradiated overnight using a 370nm UV light generator. The pH was adjusted to 9 using a sodium borate buffer solution. Standing for 30min to obtain the polylysine-polyacrylamide grafted gel-like polymer with the grafting rate of 32%.
Comparative example 1
The comparative example provides a polylysine hydrogel, which is prepared by the following preparation method:
5g of polylysine (molecular weight is 3000-5000) is weighed into a container, 50ml of ultrapure water is added for dissolution, 0.25g of EDC and 0.25g of NHS are added, and the reaction is carried out for 16 h. Co60 gamma ray 40kGy is irradiated for 12h to obtain the polylysine hydrogel.
Performance testing
(1) Mechanical Property test
The samples of examples 1 to 3 and comparative example 1 were used to prepare a hydrogel columnar sample having a size of 1cm × 2cm, and a tensile test was performed after sufficient stress relaxation at a test speed of 100mm/min to calculate the tensile elongation.
Tensile elongation (L)1-L0)/L0100% of, wherein L0Is an initial length, L1Is the stretched length.
TABLE 1 tensile elongation test results
| Test example | L0/cm | L1/cm | Tensile elongation |
| Example 1 | 2 | 24 | 1100％ |
| Example 2 | 2 | 48 | 2300％ |
| Example 3 | 2 | 32 | 1500％ |
| Comparative example 1 | 2 | 6 | 200％ |
According to the results in table 1, the polylysine-polyacrylamide hydrogel prepared by the graft modification material of the invention has mechanical properties significantly higher than those of the common polylysine hydrogel.
(2) Antibacterial experiments
The bacteria can draw up nutrients from the culture medium and grow rapidly in the culture medium, once placed in the culture medium
When the material has an antibacterial function, the bacteriostatic activity of the material causes difficulty in survival of bacteria around the material, which results in
An obvious sterile zone, also called bacteriostatic zone, appears around the sample, as shown in fig. 1.
Selecting staphylococcus aureus and escherichia coli as test strains, and adopting agar plate diffusion method to test the table
And (4) characterizing the antibacterial effect of the hydrogel. The cylindrical hydrogel is placed in the center of an agar culture medium coated with a bacterial liquid, cultured in a constant-temperature incubator at 37 ℃ for 24 hours, and the diameter of a bacteriostatic zone is measured. The antibacterial activity of the hydrogel is represented by the width (H) of the zone of inhibition. Wherein the calculation of the width (H) of the bacteriostatic band is shown as the formula 1:
(formula 1)
Wherein: d is the average value (mm) of the outer diameter of the bacteriostatic circle; d is the diameter (mm) of the hydrogel.
The samples in the embodiment 1 are selected to carry out the antibacterial experiment test, the maximum inhibition zone widths of escherichia coli and staphylococcus aureus are respectively (9.56 +/-0.13) mm and (12.35 +/-0.72) mm, and the antibacterial performance is excellent.
(3) Adhesion test
To determine adhesion, the hydrogel of the present invention was tested for its adhesive strength to gelatin by uniformly coating a thick glass slide with a layer of gelatin to simulate human tissue. The aqueous gelatin solution was uniformly coated onto 5mmx20mmx50mm glass slides. And placing the mixture in an oven at 70 ℃ for 30min to remove residual water in the gelatin, thus obtaining the required gelatin glass sheet. Then, the two gelatin glass sheets are mutually lapped, a layer of hydrogel is uniformly coated on the overlapped part and then is clamped, after the gelatin glass sheets are placed for 30min, the bonding strength of the two glass sheets is tested at room temperature by using a universal mechanical testing machine, and the chuck speed is 5 mm/min.
The hydrogels obtained in examples 1 to 3 and comparative example 1 were tested according to the above method. The results are as follows:
table 2 adhesion strength test results
| Examples of the experiments | Adhesive strength |
| Example 1 | 1.53MPa |
| Example 2 | 2.24MPa |
| Example 3 | 1.74MPa |
| Comparative example 1 | 0.32MPa |
(4) Saturated Water absorption test
A sample having a mass m0And soaked in PBS solution for 24h at 37 ℃. Taking out the gel, weighing to obtain gel mass m after soaking1The saturated water absorption was calculated as (m)1-m0)/m0*100％
TABLE 3 saturated Water absorption test results
| Sample (I) | m0 | m1 | Saturated water absorption |
| Example 1 | 1g | 4.6g | 360％ |
| Example 2 | 1g | 7.8g | 680％ |
| Example 3 | 1g | 5.2g | 420％ |
| Comparative example 1 | 1g | 2.4g | 140％ |
The hydrogel provided by the embodiments of the invention has higher saturated water absorption rate, and the saturated water absorption rate of the colloid is improved, so that the wound surface can be fully adsorbed, and the physical compression hemostasis effect is increased.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (11)
1. A method for preparing a hydrogel, comprising the steps of:
s1: adding 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide EDC and N-hydroxysuccinimide NHS into a polylysine aqueous solution, reacting for 4-8 h, dialyzing, and freeze-drying the dialyzed solution to obtain modified polylysine;
s2: dissolving the modified polylysine obtained in the step S1 in water, adding an acrylamide monomer, stirring, uniformly mixing, adding a photoinitiator ammonium persulfate and N, N' -methylene-bisacrylamide, continuously stirring, and then irradiating by ultraviolet light; the mass ratio of the modified polylysine to the acrylamide monomer is 1: 10-10: 1;
s3: then adjusting the pH value to 8-10, and standing to obtain the hydrogel; the grafting rate of polylysine in the hydrogel is 10-45%.
2. The method for preparing the hydrogel according to claim 1, wherein the mass ratio of EDC to NHS in S1 is 1: 1-1: 5, and the mass ratio of the sum of EDC and NHS to polylysine is 5: 100-10: 100.
3. The method for preparing the hydrogel according to claim 2, wherein the mass ratio of EDC to NHS is 1: 1.
4. The method for producing the hydrogel according to claim 1, wherein the modified polylysine in S2 has a mass concentration of 10 to 20% after being dissolved in water.
5. The method for preparing the hydrogel according to claim 1, wherein the mass ratio of the modified polylysine to the acrylamide monomer is 1: 1.
6. The method for preparing the hydrogel according to claim 1, wherein the mass ratio of ammonium persulfate to N, N' -methylenebisacrylamide in S2 is 1: 1; the mass ratio of the sum of the mass of the ammonium persulfate and the mass of the N, N' -methylene-bisacrylamide to the mass of the acrylamide monomer is 1: 100-5: 100.
7. The method for preparing the hydrogel according to claim 1, wherein the polylysine in S1 has a molecular weight of 3000-5000 Da.
8. The method for producing a hydrous gel as claimed in claim 1, wherein the pH in S3 is adjusted to 9.
9. The hydrogel prepared from the graft modification material is characterized in that the hydrogel is formed by polylysine grafted polyacrylamide and has an interpenetrating network structure, and is prepared by the preparation method of any one of claims 1 to 8.
10. The hydrogel according to claim 9, wherein the hydrogel has a tensile elongation of 1000 to 2500% and a saturated water absorption of 400 to 800%.
11. Use of the hydrogel according to any one of claims 9 to 10 in preparation of a chronic wound repair plugging product, a diabetic foot repair product or a surgical wound repair product.
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