CN114259319A - A line bolt for making animal cerebral arterial thrombosis model - Google Patents
A line bolt for making animal cerebral arterial thrombosis model Download PDFInfo
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- CN114259319A CN114259319A CN202111450550.XA CN202111450550A CN114259319A CN 114259319 A CN114259319 A CN 114259319A CN 202111450550 A CN202111450550 A CN 202111450550A CN 114259319 A CN114259319 A CN 114259319A
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Abstract
The invention relates to the technical field of animal experiment modeling instruments, in particular to a wire bolt for manufacturing an animal ischemic stroke model. The wire bolt for manufacturing the animal ischemic stroke model is simple in structure and low in manufacturing cost, can effectively overcome the defects caused by insufficient experience of operators, and greatly improves the molding success rate.
Description
Technical Field
The invention relates to the technical field of animal experiment modeling instruments, in particular to a wire plug for manufacturing an animal ischemic stroke model.
Background
Stroke occurs due to an interruption of blood supply to the brain area and can lead to death or permanent neurological deficits including balance problems, hemiplegia, loss of sensation and vibration sensation, numbness, impaired reflexes, ptosis, visual field deficits, aphasia, and disuse. The cerebral apoplexy can be classified into ischemic stroke or hemorrhagic stroke according to the pathological basis, and the ischemic stroke accounts for a higher proportion of the whole cerebral stroke. After ischemia, neuron cells cannot maintain normal transmembrane ion gradient and balance, so that a series of cell death processes including apoptosis, excitotoxicity, oxidative stress, inflammation and the like are caused, the pathological and physiological processes seriously damage the neuron cells, glial cells and endothelial cells, are mutually associated, mutually triggered and form malignant circulation, and finally cause apoptosis or necrosis of the neuron cells, so that clinically corresponding nerve function loss expression is generated, and the life quality of a patient is seriously influenced.
The normal blood supply to the brain is provided by the carotid and vertebral arteries. Under pathological conditions, the blood supply is reduced due to the stenosis of internal carotid artery or the stenosis of cerebral artery lumen is caused due to cerebral atherosclerosis, the acute cerebral blood supply is insufficient due to the blood circulation disturbance of brain, the local cerebral hemorheology abnormality and the hypercoagulability of blood coagulation occur, the disintegration and the damage of tissue structures such as nerve cells, fibers, blood vessels and the like in a certain range are caused, and a series of pathological processes such as biochemical metabolism disorder, tissue structure change, physiological function loss and the like occur. The pathophysiology characteristics and pathogenesis of ischemic stroke are deeply researched, and a theoretical basis is provided for establishing an animal model of the cerebral arterial thrombosis. Among them, the most commonly used experimental model for rodent ischemic stroke is the middle cerebral artery embolism model, which does not require craniotomy. It is inserted into a plug along the internal carotid artery and advanced until the middle cerebral artery is initially occluded. The wire bolt model is mainly used for a permanent ischemia model and can also be used as a focal ischemia model, and reperfusion can be realized by pulling out the wire bolt.
At present, when a wire bolt method is adopted for molding, the selection of the wire bolt, a specific operation method and the operation experience of an operator can have great influence on the success rate of molding. Firstly, when selecting the wire bolt, a wire bolt with a proper thickness is generally selected according to the type and the weight of an animal model, the selected wire bolt is moderate in hardness, and if the wire bolt is too soft, the wire bolt is not easy to insert in the molding process; if the wire plug is too hard, the blood vessel is easy to puncture during the molding process, which leads to hemorrhagic brain injury or permanent cerebral ischemia injury. Secondly, in the molding process, the insertion depth of the wire bolt is required to be controlled, and when the insertion depth of the wire bolt is insufficient, the blood flow of the ACA cannot be completely blocked, so that the animal can have no obvious neurologic impairment or light symptoms; when the insertion depth of the thread plug is too large, the subarachnoid space of the experimental animal is possibly bled, the bleeding amount of the animal is directly increased or the animal dies, and due to the difference of the administration, the growth cycle and the individual among modeling animals, even if the animals with the same weight are different in the insertion depth of the thread plug during modeling, an operator is required to flexibly control the insertion depth during modeling; in addition, because the cerebral vessels have more bifurcations, the advancing force of the wire plug needs to be paid attention to in the advancing process of the wire plug so as to avoid puncturing the vessels, and the requirement on the experience of an operator is high. The factors directly cause lower success rate and higher molding difficulty in the molding of the experimental animal.
Disclosure of Invention
In order to solve the above technical problems, an object of the present invention is to provide a wire plug for manufacturing an animal model of cerebral arterial thrombosis, which can overcome the defects in the prior art, has a moderate hardness, a smooth surface, and a rounded tip, and can be smoothly pushed along a blood vessel during molding, and the molding success rate is effectively improved.
In order to achieve the technical effect, the invention adopts the following technical scheme:
the wire bolt for manufacturing the animal ischemic stroke model comprises a head end and a tail end, wherein the diameter of the head end is smaller than or equal to the diameter of the tail end, the length of the head end is larger than or equal to the length of the tail end, at least one flexible section is arranged between the head end and the tail end, and the diameter of the flexible section is smaller than the diameter of the head end.
Further, the front end of the head end is provided with a smooth round shape, preferably a hemisphere shape.
Further, the length of the flexible section is less than the head end length.
Further, the head end comprises a raw material line positioned on the inner layer, and a coating layer is arranged outside the raw material line.
Preferably, the raw material line and the flexible section are both selected from any one of polyethylene, silica gel, rubber, silicone rubber, polyvinyl chloride, polypropylene, polytetrafluoroethylene, polyamide, ABS plastic, silk fibroin and nylon materials, and preferably, the raw material line and the flexible section are both made of the same material.
Is any one of polyethylene, silica gel, rubber, silicon rubber, polyvinyl chloride, polypropylene, polytetrafluoroethylene, polyamide, ABS plastic, silk fibroin and nylon materials.
Preferably, the coating layer is any one of silica gel, paraffin, silicone rubber, polyamide, polytetrafluoroethylene and polyurethane.
Further, the coating layer is arranged outside the coating layer, the coating layer is preferably a coating with an antibacterial effect, a hydrophilic effect or a smooth surface, specifically, a polyvinylpyrrolidone hydrophilic coating or a paraffin coating can be selected, and the thickness of the coating layer is preferably 50-300 um.
Further, the tail end raw material is any one selected from ninone, silica gel, polyethylene, polyvinyl chloride and polytetrafluoroethylene.
Furthermore, the diameter of the head end is 0.1mm-6mm, and the length of the head end is 5mm-400 mm.
Further, the length of the flexible section is 5mm-100 mm.
Further, the diameter of the flexible section is 0.1mm-4 mm.
Furthermore, at least one length mark is arranged on the head end, the length marks are preferably 3-5, and the distance from one length mark on the head end to the front end of the head end is 17mm-19 mm.
Preferably, the length mark is preferably a color mark or an inwardly recessed scale mark.
In a second aspect, the invention further provides a preparation method of the wire plug for making an animal ischemic stroke model, which specifically comprises the following steps:
s1: stretching the material into filaments, wherein one end of each filament is used as a head end raw material line, and the other end of each filament is used as a flexible section;
s2: polishing the front end of the head end raw material line into a smooth round blunt shape, then uniformly coating or wrapping a layer of coating layer on the outer part of the head end raw material line, and polishing one end of the head end raw material line, which is far away from the flexible section, into the smooth round blunt shape;
s3: coating a layer of coating layer on the outer part of the head end raw material line with the coating layer, enabling the thickness of the coating layer to be uniform so as to prepare the head end raw material line into a bolt head end, and polishing the bolt head end into a smooth round blunt shape;
s4: the flexible segment is attached to the tail end of the wire bolt.
Compared with the prior art, the invention has the beneficial effects that:
according to the first aspect, the wire bolt for manufacturing the animal ischemic stroke model is provided with the tail end and the flexible section, the tail end is held by an operator, the head end is inserted into a blood vessel to block blood flow, and the flexible section is located between the head end and the tail end.
In a first aspect, the wire plug for manufacturing an animal model with cerebral ischemic stroke provided by the invention has a head end with an inner layer structure and an outer layer structure, and the wire plug sequentially comprises a raw material wire, a coating layer and a coating layer from inside to outside, wherein the raw material wire with a proper thickness is selected, the coating layer is arranged outside the raw material wire, so that the head end can still keep good flexibility when the diameter of the head end is larger, and the coating layer is arranged outside the coating layer, so that the head end can be smoothly pushed in a blood vessel. The molding efficiency and the success rate are improved.
Drawings
Fig. 1 is a schematic overall structure diagram of a cord plug for making an animal model of ischemic stroke according to embodiment 1 of the present invention;
fig. 2 is a schematic partial enlarged structural diagram of a wire plug for making an animal model of ischemic stroke provided in embodiment 1 of the present invention at a position a;
fig. 3 is a schematic structural diagram of a wire plug for making an animal model of ischemic stroke according to embodiment 2 of the present invention;
fig. 4 is a schematic structural diagram of a wire plug for making an animal model of ischemic stroke according to embodiment 3 of the present invention;
the reference signs are: 10, tail end, 20, head end, 21, raw material line, 22, coating layer, 23, coating layer, 30, flexible section, 40 and secondary tail end.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.
Example 1
As shown in fig. 1-2, the following is a first embodiment of the present invention, specifically as follows:
a cord plug for making an animal model of ischemic stroke comprises a head end 20 and a tail end 10, wherein the head end 20 and the tail end 10 are identical in structure. Specifically, the ends of the head end 20 and the tail end 10 away from each other are both provided with smooth round blunt shapes, and the head end 20 and the tail end 10 each include a raw material line 21 located at an inner layer, a coating layer 22 located at the outer part of the raw material line 21, and a coating layer 23.
In this embodiment, the raw material line 21 is made of nylon material, the coating layer 22 is silica gel, and the coating layer 23 is a polyvinylpyrrolidone hydrophilic coating, the lengths of the head end 20 and the tail end 10 are both 5mm, and the diameters thereof are equal and are both 0.1mm to 6 mm. More specifically, the diameter of the raw material wire 21 was 0.15mm, and the thickness of the polyvinylpyrrolidone hydrophilic coating layer was 50 um. In practice, the thickness of the coating 22 can be flexibly adjusted to obtain different diameters of the head end 20 and the tail end 10 to match the animal species and body weight.
In this embodiment, the flexible section 30 is made of the same material as the material line 21 of the head end 20 and the material line 21 of the tail end 10, so as to reduce the manufacturing cost, and the flexible section 30 is made of nylon material, and has a length of 5mm to 20mm and a diameter of 0.1 mm.
In addition, in order to observe the insertion depth, three length marks are arranged on the head end 20 and the tail end 10 respectively and are marked by different colors, wherein the length mark at the middle position is 18mm +/-1 mm away from the end part of the head end 20, and the distance between the adjacent length marks is equal.
When the method is specifically implemented, firstly, the wire bolt with the proper diameter is selected according to the type and the weight of an animal, in the operation process, the head end 20 or the tail end 10 can be selected as an insertion end at will, the other end can be held, the flexible section 30 is small in diameter and thin and soft, and the resistance of the wire bolt in the advancing process can be reflected according to the bending degree of the flexible section 30 in the inserting process, so that an operator can adjust the operation force and the advancing direction according to the resistance in the advancing process, and the experience requirement of the operator is reduced.
Example 2
As shown in fig. 3, the following is a second embodiment of the present invention, specifically as follows:
the utility model provides a line bolt for making animal ischemic stroke model, includes head end 20 and tail end 10, head end 20 and tail end 10 structure and length all are different, still be provided with flexible section 30 between head end 20 and the tail end 10, specifically, the one end that tail end 10 was kept away from to head end 20 all sets up to glossy round blunt shape, head end 20 diameter is less than or equal to tail end 10 diameter, head end 20 length is greater than tail end 10 length, tail end 10 can supply to take, the front end can be used for blocking the blood vessel as inserting the section.
In this embodiment, the head end 20 and the tail end 10 each include a raw material line 21 located in an inner layer, a coating layer 22 located outside the raw material line 21, and a coating layer 23, where the raw material line 21 is polytetrafluoroethylene, the coating layer 22 is a polyurethane material, and the coating layer 23 is a polyvinylpyrrolidone hydrophilic coating. The head end 20 is 200mm long so that the length is suitable for rat modeling. The length of the tail end 10 is 20mm-40mm, the diameter of the head end 20 is 0.25mm-2mm, and the diameter of the tail end 10 is 0.25-4 mm.
In this example, the diameter of the raw material wire 21 is 0.2mm, the thickness of the polyvinylpyrrolidone hydrophilic coating layer is 100um, and the thickness of the coating layer is 0.2 mm.
In this embodiment, the flexible section 30 and the material line 21 of the head end 20 are made of the same material to reduce the manufacturing cost, and are made of polytetrafluoroethylene, and the length of the flexible section 30 is 20mm to 40mm, and the diameter is 0.2 mm.
In this embodiment, for the convenience of observing the insertion depth, five length marks are respectively arranged on the head end 20 and the tail end 10 and respectively marked by different colors, and the distances from the five length marks to the end of the head end 20 are respectively 16mm, 17mm, 18mm, 19mm and 20 mm.
In practice, a wire plug with a suitable diameter is selected according to the species and weight of the animal, the head end 20 is used as an insertion end by holding and rotating the tail end 10 during the operation, and a length mark is selected as a reference mark according to the weight and growth cycle of the animal body (preferably a rat) during the insertion process, and the insertion speed can be increased before the reference mark so as to improve the molding efficiency. When the depth of insertion reaches this reference mark, can slow down the propulsion speed of line bolt to observe flexible section 30 degree of deformation in advancing the in-process, with the resistance change when feeling the line bolt and impel, adjust line bolt advancing direction and advancing force in good time according to this resistance change, with avoid blindly impel and lead to the vascular to break, with the survival rate of guaranteeing the animal. The mode can effectively overcome the defect that the hardness of the whole wire bolt in the prior art is consistent, is convenient for an operator to sense the pressure change in the propulsion process in the operation process, and can greatly improve the survival rate of model animals.
Example 3
As shown in fig. 4, the following is a third embodiment of the present invention, specifically as follows:
a wire bolt for manufacturing an animal ischemic stroke model comprises a tail end 10, a flexible section 30, a secondary tail end 40 and a head end 20 which are sequentially arranged, wherein the tail end 10 and the secondary tail end 40 are identical in length and structure and can be held, and the head end 20 can be inserted into a blood vessel to block the blood vessel, so that the cerebral ischemic model is built.
In this embodiment, the ends of the head end 20 away from the tail end 10 are both provided with smooth round blunt shapes, and the diameter of the head end 20 is equal to the diameter of the tail end 10 and the diameter of the secondary tail end 40. Specifically, head end 20 is including being located the raw materials line 21 of inlayer, being located coating 22 and the dope layer 23 of the outside of raw materials line 21, wherein, raw materials line 21 is polytetrafluoroethylene or nylon materials, coating 22 is polyurethane or silica gel material, and dope layer 23 is the hydrophilic coating of polyvinylpyrrolidone. The length of the head end 20 is 200mm-400mm, the length of the tail end 10 is 20mm-50mm, the diameter of the head end 20 is 2mm-6mm, and the diameter of the tail end 10 is 2mm-8 mm.
In this example, the diameter of the raw material line 21 is 0.2mm to 1.8mm, and the thickness of the polyvinylpyrrolidone hydrophilic coating layer is 300 um. In practice, the thickness of the coating 22 can be flexibly adjusted to obtain different diameters of the head end 20 and the tail end 10 to match the animal species and body weight.
In this embodiment, the flexible section 30 and the material line 21 of the head end 20 are made of the same material, and are made of polyamide material, and the length of the flexible section 30 is 40mm to 100mm, and the diameter is 0.2mm to 1 mm.
In the present embodiment, for the convenience of observing the insertion depth, five length marks are respectively arranged on the head end 20 and the tail end 10, and are marked by concave scales, and the distances from the five length marks to the end of the head end 20 are respectively 14mm, 16mm, 18mm, 20mm and 22 mm.
In the concrete implementation, firstly, the wire bolt with the proper diameter is selected according to the type and the weight of the animal, one length mark is selected as a reference mark, in the early stage of operation, the secondary tail end 40 can be held and the part in front of the reference mark can be quickly placed into the blood vessel, when the insertion depth is close to the reference mark, the placing speed is slowed down, the tail end 10 is held, the resistance change of the wire bolt in the advancing process is judged by observing the deformation degree of the flexible section 30, and the advancing direction and the advancing force of the wire bolt are timely adjusted according to the resistance change, so that the blood vessel is prevented from being inserted, and the survival rate and the molding success rate of the model animal are improved.
Example 4
The method is characterized in that 68 male mice of SD rats (with the weight of 270g-285g and the age of 50-52d) are randomly divided into a control group and an experimental group for MCAO molding, wherein the control group adopts rats of Guangzhou Jialing biotechnology limited to mold a wire plug, the experimental group adopts the wire plug prepared in the embodiment 2 to mold, and the molding success rate is shown in the following table 1:
table 1: the power of the experimental group and the molding composition is compared as follows:
although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the invention as defined in the appended claims. The techniques, shapes, and configurations not described in detail in the present invention are all known techniques.
Claims (7)
1. A wire bolt for manufacturing an animal model of cerebral ischemic stroke is characterized in that: including head end (20) and tail end (10), head end (20) diameter is less than or equal to tail end (10) diameter, just be provided with one flexible section (30) between head end (20) and tail end (10) at least, the diameter of flexible section (30) is less than head end (20) diameter, head end (20) are including raw materials line (21) that are located the inlayer, the outside of raw materials line (21) is equipped with coating (22).
2. The wire plug for making an animal model of ischemic stroke of claim 1, wherein: the raw material line (21) and the flexible section (30) are made of any one of polyethylene, silica gel, rubber, silicon rubber, polyvinyl chloride, polypropylene, polytetrafluoroethylene, polyamide, ABS plastic, silk fibroin and nylon materials.
3. The wire plug for making an animal model of ischemic stroke of claim 1, wherein: the coating layer (22) is any one of silica gel, paraffin, silicone rubber, polyamide, polytetrafluoroethylene and polyurethane.
4. The wire plug for making an animal model of ischemic stroke as claimed in claim 3, wherein: and a coating layer (23) is arranged outside the coating layer (22).
5. The wire plug for making an animal model of ischemic stroke of claim 1, wherein: the diameter of the head end (20) is 0.1mm-6mm, the length of the head end (20) is 5mm-400mm, the length of the flexible section (30) is 5mm-100mm, and the diameter of the flexible section (30) is 0.1mm-4 mm.
6. The wire plug for making an animal model of ischemic stroke of claim 1, wherein: the head end (20) is provided with at least one length mark.
7. The method for preparing the wire plug for the animal model of ischemic stroke as claimed in claim 1, comprising the steps of:
s1: stretching the material into filaments, one end of the filaments being used as a head end (20) raw material line (21) and the other end being used as a flexible section (30);
s2: polishing the front end of a raw material line (21) of the head end (20) into a smooth round blunt shape, then uniformly coating or wrapping a coating layer (22) on the outer part of the raw material line (21) of the head end (20), and polishing one end of the raw material line, which is far away from the flexible section (30), into a smooth round blunt shape;
s3: coating a layer of coating layer (23) on the outer portion of a head end (20) raw material line (21) with a coating layer (22) to enable the thickness of the coating layer (23) to be uniform so as to prepare the head end (20) raw material line (21) into a bolt head end (20), and polishing the bolt head end (20) into a smooth round blunt shape;
s4: connecting the flexible section (30) to the wire bolt tail end (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202111450550.XA CN114259319A (en) | 2021-12-01 | 2021-12-01 | A line bolt for making animal cerebral arterial thrombosis model |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111450550.XA CN114259319A (en) | 2021-12-01 | 2021-12-01 | A line bolt for making animal cerebral arterial thrombosis model |
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| CN114259319A true CN114259319A (en) | 2022-04-01 |
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| CN202111450550.XA Pending CN114259319A (en) | 2021-12-01 | 2021-12-01 | A line bolt for making animal cerebral arterial thrombosis model |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050283182A1 (en) * | 2004-06-21 | 2005-12-22 | Concentric Medical, Inc. | Systems and methods for intraluminal delivery of occlusive elements |
| CN104887340A (en) * | 2015-05-29 | 2015-09-09 | 河南科技大学第一附属医院 | Manufacturing method of sutures used for MACO models of mice |
| CN204744287U (en) * | 2015-04-07 | 2015-11-11 | 深圳市第二人民医院 | A line is tied for making big, mouse ischemic cerebral apoplexy model |
| CN111870303A (en) * | 2020-08-06 | 2020-11-03 | 聚辉医疗科技(深圳)有限公司 | Embolization device |
| CN216628805U (en) * | 2021-12-01 | 2022-05-31 | 北京市神经外科研究所 | A line bolt for making animal cerebral arterial thrombosis model |
-
2021
- 2021-12-01 CN CN202111450550.XA patent/CN114259319A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050283182A1 (en) * | 2004-06-21 | 2005-12-22 | Concentric Medical, Inc. | Systems and methods for intraluminal delivery of occlusive elements |
| CN204744287U (en) * | 2015-04-07 | 2015-11-11 | 深圳市第二人民医院 | A line is tied for making big, mouse ischemic cerebral apoplexy model |
| CN104887340A (en) * | 2015-05-29 | 2015-09-09 | 河南科技大学第一附属医院 | Manufacturing method of sutures used for MACO models of mice |
| CN111870303A (en) * | 2020-08-06 | 2020-11-03 | 聚辉医疗科技(深圳)有限公司 | Embolization device |
| CN216628805U (en) * | 2021-12-01 | 2022-05-31 | 北京市神经外科研究所 | A line bolt for making animal cerebral arterial thrombosis model |
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