CN104415402A - Three-dimensional patch used for nerve regeneration and dura mater repairing - Google Patents
Three-dimensional patch used for nerve regeneration and dura mater repairing Download PDFInfo
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- CN104415402A CN104415402A CN201310361880.0A CN201310361880A CN104415402A CN 104415402 A CN104415402 A CN 104415402A CN 201310361880 A CN201310361880 A CN 201310361880A CN 104415402 A CN104415402 A CN 104415402A
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- dura mater
- sticking patch
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- neuranagenesis
- dimensional patch
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Abstract
The invention aims to provide a three-dimensional patch used for nerve regeneration and dura mater repairing. A supporting layer is composed of regenerated-base bacterial cellulose being high in Young modulus, being high in tensile strength and being excellent in shape maintaining capability in a knitting manner. A middle layer is composed of anti-microbial-enhanced chitosan membrane. An outer layer is composed of crosslinked sodium hyaluronate being antiblocking, being strong in water binding capacity and being water-permeable and breathable. In addition, the bacterial cellulose is excellent in water binding capacity and the water-permeable and breathable performance and contains internally not conventional circular pores but microchannels in different sizes and large pores so that neutrophil in body can pass through the pores of a repairing net and bacteria being liable to cause infection can be phagocytosed. The three-dimensional patch is beneficial to healing of injury parts without causing infection in the injury parts. The three-dimensional patch is free of reoperation for being taken out since the three-dimensional patch is degradable fiber so that pains in patients can be reduced.
Description
Technical field
Patent of the present invention relates to a kind of three-dimensional sticking patch repaired for neuranagenesis and dura mater, and the nerve particularly prepared by tissue engineering technique and dura mater sticking patch fabric are applied in medical non-supported tissue and dural treatment aspect.
Background technology
Cellulose is a kind of attractive natural biological high polymer material, and its fibre structure is consistent with the collagen fiber of composition nerve head in morphology.The microstructure support of its mutual UNICOM has high porosity, is the ideal chose that neuranagenesis and dura mater are repaired.The feature of the extracellular matrix of its architectural feature of nanofiber and tissue is similar, has high specific surface area, sticking and growing advantageously in cell, has better cell adhesion ability and cytoactive, can act on tissue engineering nerve material.Bacterial cellulose has splendid shape maintains ability and anti-tear power.In natural environment, finally can explain into the small-molecule substances such as monosaccharide under the conditions such as acidity, microorganism and cellulase catalytic; The Modulatory character of performance and shape during Bacterial cellulose biosynthesis.By regulating condition of culture, the discrepant Bacterial cellulose of chemical property can be obtained.Meanwhile, it have good biocompatibility, biodegradability, biocompatibility, without anaphylaxis, and high retentiveness and degree of crystallinity, good nanofiber network, high tension force and intensity, especially good mechanical tenacity.Therefore, in tissue engineering bracket, artificial blood vessel, artificial skin and treatment skin injury, transmitting tissue's reparation etc., tool has been widely used, and is one of focus of international bio medical material research.The degraded of its nanofiber creates more space and high porosity for neuranagenesis and dura mater reparation provides interconnective passage, advantageously in the absorption of nervous tissue and dura mater growth and convenient bone cement.But Bacterial cellulose be degraded to acidic materials, long term accumulation can cause acidosis.Chitosan is a kind of avirulence, non-irritating very safe machine body material.Containing a large amount of amino and oh group in chitosan molecule, there is good cellular affinity.The existence of amino group makes chitosan have good hydrophilic, also makes chitosan with alkalescence, thus can make up the acidosis of Bacterial cellulose.Separately will have the repair materials of nanofibrous structures, and by cross-linked surface modification, in repair materials, introduce hyaluronic peptide group, with the hydrophilic of decorative material and cellular affinity, make the requirement that prepared repair materials reaches desirable.
Summary of the invention
Comprise in nerve and cerebral dura mater prosthesis higher to the requirement of repairing in non-supported tissue repair, require that there is splendid shape maintains ability and anti-tear power, have good biocompatibility, biodegradability, biocompatibility, without anaphylaxis, and high retentiveness and degree of crystallinity, good nanofiber network, high tension force and intensity etc.Solve in the prosthesis of the non-supported such as nerve and cerebral dura mater tissue the above-mentioned technical problem existed, and a kind of three-dimensional sticking patch repaired for CO2 laser weld and dura mater is provided.
For achieving the above object, the embodiment of patent of the present invention " a kind of three-dimensional sticking patch repaired for neuranagenesis and dura mater " is as follows:
3, (1) neuranagenesis and dura mater repair the supporting layer of sticking patch: regenerated bacterial cellulose fiber is by weaving the obtained internal layer supporting layer being of a size of the three-dimensional sticking patch of (0.1 ~ 100) cm × (0.1 ~ 100) cm × (0.02 ~ 2) cm;
(2) intermediate layer has the chitosan film composition of antibacterial enhancing; Outer by having tissue adhesion, the crosslinked sodium hyaluronate composition of extremely strong retentiveness and water-permeable and air permeable;
Described chitosan deacylated tRNA degree is 25% ~ 95%, and molecular weight is 50,000 ~ 3,000,000;
Described hyaluronic acid or its salt by extracting or being obtained by bacterial fermentation from microorganism;
(3) ultimate strength of the three-dimensional nerve reparation of inventing and the sticking patch of dura mater reparation is 500 ~ 700CN, elongation at break is 10 ~ 30%, knotting ultimate strength is 400 ~ 600CN, knotting elongation at break is 10 ~ 50%, tensile strength is 500 ~ 1000MPa, average void is 14.5 μm, and voidage is 72 ~ 95%.
(4) advise analyzing and selecting suitable medical regenerated bacteria cellulose to repair the support raw material of sticking patch as the CO2 laser weld of CO2 laser weld and dura mater by physical test and biologic test according to clinical requirement and doctor.
A). repair according to data of literatures test and Selection the organizational structure that neurolemma repairs, three-dimensional sticking patch technological parameter, and it is not enough to separate regenerated bacteria cellulose mechanical strength, a difficult problem for not antibacterial and adhesion.
B). the Main physical performance indications such as density, thickness, tension failure brute force, bursting strength, bending resistance length, porosity of measuring sticking patch, analyze each factor and dependency relation between sticking patch physical properties index is repaired on the impact of sticking patch Main physical performance indications and CO2 laser weld and dura mater.
C). on the basis of orthogonal test, by converting the technological parameter of fabric heat-setting, analyze the impact of thermal finalization Final finishing on CO2 laser weld and dura mater repairing performance index.According to every Main physical performance indications that clinical CO2 laser weld and dura mater are repaired, the parameter factors of choice experiment, makes its every Fundamental Physical Properties index meet or exceed the standard of same kind of products at abroad.Complete the inspection of medical apparatus and instruments Quality Supervision and Inspection Center of State Food and Drug Administration.
Beneficial effect:
The object of type of the present invention is that providing a kind of has a kind of three-dimensional sticking patch repaired for neuranagenesis and dura mater of high-strength machinery, and internal layer is woven by the regeneration base Bacterial cellulose with high Young's modulus, high-tensile and splendid shape maintains ability and forms; Intermediate layer has the chitosan film composition of antibacterial enhancing; Outer by having tissue adhesion, the crosslinked sodium hyaluronate composition of extremely strong retentiveness and water-permeable and air permeable.
The rigidity of regeneration base Bacterial cellulose is relatively large, is more suitable for knitting out having some strength and bending rigidity, the neuranagenesis resisting human body abdominal pressure and defect body pressure and dura mater repair sticking patch.In addition, bacterial fibers have extremely strong retentiveness and water-permeable and air permeable.What its inside existed is not traditional circular aperture, but the minim channel of different size and larger hole can ensure the hole of the neutrophilic leukocyte in human body by repairing net, engulf the antibacterial easily causing infection, the healing at the position that is conducive to being wound and not easily cause the infection at position of being wound.Owing to being that degradable fiber does not need operation again to take out, reduce the misery of patient.
Accompanying drawing explanation
The structural representation of a kind of three-dimensional sticking patch repaired for neuranagenesis and dura mater of Fig. 1 patent of invention, 1 intermediate layer; 2 supporting layers; 3 is outer.
The structure and morphology of supporting layer fiber prepared by Fig. 2 embodiment of the present invention 1.
Detailed description of the invention
Below in conjunction with specific embodiment, set forth patent of the present invention further.Should be understood that these embodiments are only not used in for illustration of patent of the present invention the scope limiting patent of the present invention.In addition should be understood that, after the content of reading patent teaches of the present invention, those skilled in the art can make various changes or modifications patent of the present invention, these equivalent form of values fall within the application's appended claims limited range equally.
Embodiment 1
Be as shown in Figure 1 one prepared by the embodiment of the present invention 1 for neuranagenesis and the three-dimensional sticking patch of dura mater, internal layer (supporting layer) is woven by the regeneration base Bacterial cellulose with high Young's modulus, high-tensile and splendid shape maintains ability and forms; The obtained supporting layer being of a size of the three-dimensional sticking patch of 8cm × 8cm × 0.1cm of braiding; The deacetylation 75% that intermediate layer coated with antimicrobial strengthens, molecular weight is 2,000,000 chitosan films; Outer by having tissue adhesion, the hyaluronate sodium composition of the crosslinked modification of extremely strong retentiveness and water-permeable and air permeable.
The ultimate strength of sticking patch is 500CN, and elongation at break is 30%, and knotting ultimate strength is 400CN, and knotting elongation at break is 20%.
Embodiment 2
Be as shown in Figure 1 one prepared by the present invention for neuranagenesis and the three-dimensional sticking patch of dura mater, supporting layer is woven by the regeneration base Bacterial cellulose with high Young's modulus, high-tensile and splendid shape maintains ability and forms; The obtained supporting layer being of a size of the three-dimensional sticking patch of 15cm × 4cm × 0.5cm of braiding; Intermediate layer has the deacetylation 95% of antibacterial enhancing, and molecular weight is 1,500,000 chitosan compositions; Outer by having tissue adhesion, the hyaluronate sodium composition of the crosslinked modification of extremely strong retentiveness and water-permeable and air permeable.
The ultimate strength of sticking patch is 700CN, and elongation at break is 10%, and knotting ultimate strength is 600CN, and knotting elongation at break is 10%.
Embodiment 3
1, a kind of manufacturing process of the three-dimensional sticking patch repaired for neuranagenesis and dura mater: the cellulosic organizational structure of braiding regenerated bacteria of the cellulosic support of regenerated bacteria, weaving textile technological parameter
Being the one of knitting method through compiling, being employing one group or several groups of yarns arranged in parallel, on all working pin of warp-wise feeding knitting machine, carrying out lopping simultaneously and form knitted fabric.Require to analyze from the physical and mechanical properties of repairing net, the structure of warp-knitting tissue must have the crucial performance indications of enough intensity, enough porositys, suitable rigidity etc. several.
2, a kind of technological parameter of thermal finalization of the three-dimensional sticking patch repaired for neuranagenesis and dura mater
Heat-setting process will cause the change of Fiber super-molecular structure and morphosis, and the construction unit making degree of stability low changes the higher construction unit of degree of stability into.Therefore thermal finalization can accomplish the imperfection structure that formed in the improvement fiberizing of repairing formula or drawing process, instead of destroys thoroughly and rebuild.The technological parameter of sticking patch thermal finalization is as follows.
3, a kind of three-dimensional nerve regeneration of the present embodiment 1 repairs sticking patch with dura mater, and physical property is:
(1) ultimate strength is 500CN, and elongation at break is 30%
(2) longitudinal fracture is powerful: 50N, density: 42.5/5cm
(3) cross-breaking strength: 50N, lateral density: 30.5/5cm
(4) tensile strength: 720MPa
(5) elastic modelling quantity is 1.74GPa
(6) space: 14.5 μm
(7) voidage: 92%.
Claims (3)
1. the three-dimensional sticking patch repaired for neuranagenesis and dura mater, the internal layer that it is characterized in that for the three-dimensional sticking patch of neuranagenesis and dura mater reparation is woven by bacteria cellulose fibre and forms, intermediate layer applies chitosan molecule film by positive and negative chemical bond, the three-dimensional sticking patch that skin is modified by cross-linking hyaluronic acid and prepared;
Described hyaluronic acid or its salt by extracting or being obtained by bacterial fermentation from microorganism;
The deacylated tRNA degree of described chitosan is 25% ~ 95%, and molecular weight is 50,000 ~ 3,000,000.
2. a kind of three-dimensional sticking patch repaired for neuranagenesis and dura mater according to claim 1, it is characterized in that internal layer is woven by bacteria cellulose fibre to form, internal layer, as supporting layer, is of a size of (0.1 ~ 100) cm × (0.1 ~ 100) cm × (0.02 ~ 2) cm.
3. a kind of three-dimensional sticking patch repaired for neuranagenesis and dura mater according to claim 1-2, it is characterized in that the ultimate strength of three-dimensional sticking patch is 500 ~ 700CN, elongation at break is 10 ~ 30%, knotting ultimate strength is 400 ~ 600CN, knotting elongation at break is 10 ~ 50%, tensile strength is 500 ~ 1000MPa, and average void is 14.5 μm, and voidage is 72 ~ 95%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310361880.0A CN104415402A (en) | 2013-08-19 | 2013-08-19 | Three-dimensional patch used for nerve regeneration and dura mater repairing |
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| CN201310361880.0A CN104415402A (en) | 2013-08-19 | 2013-08-19 | Three-dimensional patch used for nerve regeneration and dura mater repairing |
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| CN104415402A true CN104415402A (en) | 2015-03-18 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106552294A (en) * | 2015-09-25 | 2017-04-05 | 上海市东方医院 | A kind of biology patching material for cardiac repair |
| CN107715178A (en) * | 2017-11-09 | 2018-02-23 | 李瑞锋 | A kind of multilayer contains high intensity artificial dura mater of cell factor and preparation method thereof |
| CN107913435A (en) * | 2016-10-10 | 2018-04-17 | 北京邦塞科技有限公司 | Compound hard brain (ridge) membrane implant and its preparation method and application |
| CN108066043A (en) * | 2017-12-22 | 2018-05-25 | 山东赛克赛斯生物科技有限公司 | A kind of medical embedded sticking patch and preparation method and application |
| CN110639065A (en) * | 2019-09-18 | 2020-01-03 | 北京科技大学 | Bacterial cellulose-based asymmetric bilateral anisotropic biological patch and preparation method thereof |
-
2013
- 2013-08-19 CN CN201310361880.0A patent/CN104415402A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106552294A (en) * | 2015-09-25 | 2017-04-05 | 上海市东方医院 | A kind of biology patching material for cardiac repair |
| CN106552294B (en) * | 2015-09-25 | 2021-06-01 | 上海市东方医院 | Biological patch material for heart repair |
| CN107913435A (en) * | 2016-10-10 | 2018-04-17 | 北京邦塞科技有限公司 | Compound hard brain (ridge) membrane implant and its preparation method and application |
| CN107913435B (en) * | 2016-10-10 | 2022-09-09 | 北京邦塞科技有限公司 | Composite type dura mater (spinal) membrane implant, preparation method and use thereof |
| CN107715178A (en) * | 2017-11-09 | 2018-02-23 | 李瑞锋 | A kind of multilayer contains high intensity artificial dura mater of cell factor and preparation method thereof |
| CN107715178B (en) * | 2017-11-09 | 2020-10-09 | 李瑞锋 | Multilayer high-strength artificial dura mater containing cell factors and preparation method thereof |
| CN108066043A (en) * | 2017-12-22 | 2018-05-25 | 山东赛克赛斯生物科技有限公司 | A kind of medical embedded sticking patch and preparation method and application |
| CN110639065A (en) * | 2019-09-18 | 2020-01-03 | 北京科技大学 | Bacterial cellulose-based asymmetric bilateral anisotropic biological patch and preparation method thereof |
| CN110639065B (en) * | 2019-09-18 | 2021-03-30 | 北京科技大学 | Bacterial cellulose-based asymmetric bilateral anisotropic biological patch and preparation method thereof |
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Application publication date: 20150318 |
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