CN104887340A - Manufacturing method of sutures used for MACO models of mice - Google Patents
Manufacturing method of sutures used for MACO models of mice Download PDFInfo
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- CN104887340A CN104887340A CN201510285540.3A CN201510285540A CN104887340A CN 104887340 A CN104887340 A CN 104887340A CN 201510285540 A CN201510285540 A CN 201510285540A CN 104887340 A CN104887340 A CN 104887340A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D1/00—Surgical instruments for veterinary use
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/048—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/08—Materials for coatings
- A61L31/10—Macromolecular materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/16—Biologically active materials, e.g. therapeutic substances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L33/00—Antithrombogenic treatment of surgical articles, e.g. sutures, catheters, prostheses, or of articles for the manipulation or conditioning of blood; Materials for such treatment
- A61L33/0005—Use of materials characterised by their function or physical properties
- A61L33/0011—Anticoagulant, e.g. heparin, platelet aggregation inhibitor, fibrinolytic agent, other than enzymes, attached to the substrate
- A61L33/0041—Anticoagulant, e.g. heparin, platelet aggregation inhibitor, fibrinolytic agent, other than enzymes, attached to the substrate characterised by the choice of an antithrombatic agent other than heparin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L33/00—Antithrombogenic treatment of surgical articles, e.g. sutures, catheters, prostheses, or of articles for the manipulation or conditioning of blood; Materials for such treatment
- A61L33/06—Use of macromolecular materials
- A61L33/068—Use of macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/42—Anti-thrombotic agents, anticoagulants, anti-platelet agents
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Abstract
Provided is a manufacturing method of sutures used for MACO models of mice. The sutures are prepared by selection, preparation of material, coating of head ends and marking after coating. The suture material is a filament which is drawn with the diameter ranging from 0.065 to 0. 1 mm at the temperature of 300 DEG C and made by polytetrafluoroethylene resin. At the temperature of 350 DEG C, the surface of the above filament is coated by titanium dioxide in order to obtain the material of the sutures. Coating material, for coating the head ends, is formed by mixing 15% of silicone emulsion, 34% of polyvinylpyrrolidone, 23% of polyvinyl alcohol, 16% of polyethylene glycol, and 12% of polytetrafluoroethylene under ultrasonic concussion based on weight. The manufacturing method of the sutures used for MACO models of mice has following beneficial effects: the sutures are consistent in size and have appropriate hardness and elasticity and blunt and smooth head ends so that the sutures can smoothly enter middle cerebral arteries of mice; inserted sutures can be bent in accordance with the bending blood vessels in order to reduce mechanical damage to blood vessels; obstruction is performed more completely and reliably; the scope of cerebral ischemia is stable; cerebral infarct volume is stable; operation is carried out easily; high success rate of models is achieved; and death rate of mice is low.
Description
Technical field
The invention belongs to life science, be specifically related to a kind of manufacture method of the line bolt for mouse MCAO model.
Background technology
Apoplexy is a kind of general name of acute cerebrovascular disease, and clinical manifestation is the symptom such as hemiplegia, aphasia, has the features such as high fatality rate, high disability rate and high relapse rate.The current research display apoplexy life of serious threat people and quality of life, wherein especially rank patient first with cerebral infarction, account for about 80% of all patients with cerebral apoplexy.
Cerebral infarction animal model is the basis of research cerebral ischemia and treatment, middle cerebral artery is the predilection site of cerebral infarction, therefore often adopt middle cerebral artery thromboembolism (MCAO) animal model as Research foundation, wherein line brush middle cerebral artery cerebral ischemic model is the first-selected modeling method of research ischemic brain injury animal model, focal cerebral ischemia/reperfusion injury in rats animal model the most widely studied at present by this model, has without the need to opening the advantages such as cranium, wound is minimum, cerebral ischemia position is more constant, cerebral infarction is consistent with clinical pathology process.But, this model is used for the research of rat all the time, but due to rat have that cost is high, volume is large, to raise particularly single reagent use cost be that the decades of times of mice is even more in inconvenience, and not meeting the factors such as cerebral ischemia gene level research, increasing research adopts mice to prepare this model.But because operation technique is complicated, mice is small, tolerance to the operation is poor, adds the difficulty preparing cerebral ischemic model, its stability and reliability are also key issues urgently to be resolved hurrily.But as long as we find that line bolt is carried out under study for action, little to blood vessel mechanical damage, cerebral infarction regional stability, infarct volume is stablized, and reproducible, model success rate is just close to 100%, and postoperative survival rate is also very high.Therefore, MCAO line bolt prepares the very crucial experiment material of local cerebral ischemia model.
At present, the manufacture method of MCAO line bolt has employing single line nylon wire, Mus palpus, setline etc., but the common single line nylon wire of multiselect certain diameter, at head end bag by certain silica gel.But common single line nylon wire soft durometer is not ideal enough, in the liquid of uniform temperature, easy deliquescing, easily stops in blood vessel crook, enters cranium more difficult, and the difficulty considerably increasing plug wire makes model success rate decline, and too increases hemorrhage and mortality risk; Although nylon wire must be replaced to have good elasticity and hardness compared with nylon wire with Mus, Mus palpus diameter disunity, not easily processes its head end, is difficult to cause total blockage., in modeling process, the damage of blood vessel is reduced to minimum to the processing intent of bolt the end of a thread end, the long-term surviving rate etc. that focus of infarct is more stable and higher.Conventional gluing method has parafilm wrap, silica gel bag quilt, polyhexene alcohol bolt line etc., wherein easily the coming off of parafilm wrap; Though silica gel bag is relatively stable by the line bolt infarct size gone out, but does not have expansion effect, make infarction incomplete, and mortality rate is higher, not easily long-time observational study; Polyhexene alcohol line bolt hardness runs into blood not simultaneously and can expand, and makes inlet wire not smooth.
Summary of the invention
The object of this invention is to provide a kind of manufacture method of line bolt of mouse MCAO model, and then improve the characteristic of line bolt, making it to be more suitable for for setting up mouse MCAO model, improve the postoperative survival rate of mice, reduce manufacture difficulty and the cost of MCAO model.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of manufacture method of line bolt of mouse MCAO model, this line bolt by the bag of the selection of material and preparation, the former the end of a thread end of line bolt by and wrap by after labelling obtain, its concrete step is as follows:
(1) selection of material and preparation
Polyflon being pulled at 300 DEG C diameter is after the filament of 0.065 ~ 0.01mm, under 350 DEG C of conditions, obtain in this filament surface the former line of line bolt making line bolt by titanium dioxide-coated, then according to weight by the silicon emulsion of 15%, 34% polyvinylpyrrolidone, the polyvinyl alcohol of 23%, the Polyethylene Glycol of 16% and 12% politef at 250 ~ 300 DEG C, after ultrasonic vibration, be mixed to form capsulating material for wrapping quilt;
(2) the bag quilt of the former the end of a thread end of line bolt
First 20 ± 1.0mm is intercepted as the former line line segment of line bolt from the obtained former line of line bolt, positive round is processed at these line segment two ends and is consistent with the diameter of the former thread end of described line bolt, then select wherein one end as the head end of the former line of line bolt, this head end is embedded in the capsulating material obtained by step (1), then the head end of former for line bolt line pull-out capsulating material can be completed the bag quilt to the former the end of a thread end of line bolt;
(3) bag by after labelling
Head end bag by after the former line line segment of line bolt on, the end 11 ~ 12mm of distance bag quilt, carries out labelling in one week around the former line line segment of line bolt, then former for line bolt line line segment is dried the line bolt that can obtain mouse MCAO model.
Carrying out in the process of bag quilt to the former the end of a thread end of line bolt, former for line bolt the end of a thread end after capsulating material stops 1 ~ 3s, then is pulled out capsulating material by the former the end of a thread end of line bolt.
Line bolt made by the present invention can be immersed in the normal saline containing heparin sodium for subsequent use before use, to reduce the thrombosis around line bolt.
Compared with prior art, the present invention has following beneficial effect:
First, the line bolt that the present invention does is in the same size, hardness elasticity is moderate, line bolt head end circle is blunt smooth, make line bolt can enter middle cerebral artery smoothly, and the line bolt after inserting can with the bending of blood vessel, decrease the mechanical damage to blood vessel, block more thoroughly more reliable, cerebral ischemia range stabilises, cerebral infarct volume and stablize, simple to operate, model success rate is high, and mouse death rate is low;
Second, the present invention adopts and first wraps by the method for rear labelling, carry out capsulating material in the process of bag quilt after can avoiding first labelling and can block the situation of even overlay marks, and when wrapping quilt after first labelling, due to the number of capsulating material, make distance between the end of bag quilt and labelling can distance between greater than flag tense marker place and line bolt head end end, and then affect when plug wire the judgement of loop length, and adopt the present invention first to wrap by the method for rear labelling, then can avoid this problem well, control the length of inlet wire accurately;
3rd, the former line of line bolt that the present invention does is non-hollow billet, smooth degree is close to real silk, very faint to tissue injury, and be coated with titanium dioxide, under illumination condition, can there is redox reaction in titanium dioxide, there is the effect of sterilization, make the line bolt after making better keep aseptic condition;
4th, the present invention's capsulating material used is a kind of transparent polymer anti-biotic material, the intracranial infection probability of mice can be reduced, and the stimulation that can reduce blood vessel, thus the survival rate of mice after raising modelling, and, in the raw material making this transparent polymer anti-biotic material, silicon emulsion can reduce the surface tension of material, and Polyethylene Glycol makes capsulating material have certain anticoagulant;
5th, this bag is by process, the head end of the former line of line bolt stops 1 ~ 3s in capsulating material, the head end absorption capsulating material of the former line of line bolt can be conducive to, make it have certain Hardness and toughness, and the combination between capsulating material and the former line of line bolt is tightr, in the process of analogue formation, there will not be bag by situation about coming off, if but overstand, the line bolt head end hardness of making then can be made comparatively large, more easily mechanical damage is caused to blood vessel in the process inserting blood vessel, be unfavorable for the making of model;
6th, in use, after line bolt soaks in heparin sodium, the formation of thrombosis around line bolt can be reduced, favourable extracting with line bolt when pulling out line Reperfu-sion, and blood flow can be kept after pulling out Outlet bolt unobstructed, can not because formation thrombosis blocking blood flow.
Detailed description of the invention
Below by detailed description of the invention, the present invention is further illustrated.
Embodiment 1: a kind of manufacture method of line bolt of mouse MCAO model, by make selection and the preparation of material, head end bag by and wrap by after labelling, finally obtain the line bolt being applicable to mouse MCAO model making, its concrete manufacture method is as follows:
1, selection and the preparation of material is made
1.1, line bolt material
Polyflon being pulled at 300 DEG C diameter is after the filament of 0.065mm, under 350 DEG C of conditions, obtain in this filament surface the former line making line bolt by titanium dioxide-coated, the natural tension force of the made former line of line bolt is 1.96N, and drawing tension is 1.67 ± 0.78 N;
1.2, the capsulating material of head end
Capsulating material for line bolt head end bag quilt is transparent polymer anti-biotic material, this transparent polymer anti-biotic material be by the silicon emulsion of 15%, 34% polyvinylpyrrolidone, the polyvinyl alcohol of 23%, the Polyethylene Glycol of 16% and 12% politef mix after ultrasonic vibration at 250 DEG C;
2, head end bag quilt
2.1, the processing of the former line of line bolt
The former line of above-mentioned made line bolt is placed under the microscope, after measuring the diameter of its end, vertical intercepted length is the former line line segment of line bolt of 19mm, and positive round is processed at the two ends of former for the line bolt obtained line line segment, and the diameter of its diameter and the former thread end of line bolt before processing is consistent;
2.2, bag quilt
Capsulating material obtained in step 1.2 is placed on the glass planar under microscope, then one end of the former line line segment of the line bolt obtained by machining in step 2.1 is elected to be head end, and use microforceps to clamp the former line line segment of line bolt, its head end is embedded in capsulating material, draw and pull it capsulating material rapidly after stopping 1s, can complete the bag quilt to head end, and bag is 5mm by the length of part;
3, wrap by after labelling
3.1, labelling
Step 2.2 is wrapped by after the former line line segment of line bolt be placed in microscope under, slide gauge is used to carry out labelling at the end 11mm of distance bag quilt, and institute makes marks will around the former line line segment of line bolt one week, so as accurately to judge and adjustment inlet wire direction and control the degree of depth of inlet wire, thus make the line bolt after making to enter outside neck smoothly;
3.2, dry
The former line line segment of line bolt through labelling is dried the line bolt finished product that can obtain for mouse MCAO model.
Embodiment 2: a kind of manufacture method of line bolt of mouse MCAO model, by make selection and the preparation of material, head end bag quilt and wrap by after labelling, finally obtain the line bolt being applicable to mouse MCAO model, its concrete manufacture method is as follows:
1, selection and the preparation of material is made
1.1, line bolt material
Polyflon being pulled at 300 DEG C diameter is after the filament of 0.1mm, under 350 DEG C of conditions, obtain in this filament surface the former line making line bolt by titanium dioxide-coated, the natural tension force of the made former line of line bolt is 1.88N, and drawing tension is 1.71 ± 0.82 N;
1.2, the capsulating material of head end
Capsulating material for line bolt head end bag quilt is transparent polymer anti-biotic material, this transparent polymer anti-biotic material be by the silicon emulsion of 15%, 34% polyvinylpyrrolidone, the polyvinyl alcohol of 23%, the Polyethylene Glycol of 16% and 12% politef mix after ultrasonic vibration at 300 DEG C;
2, head end bag quilt
2.1, the processing of the former line of line bolt
The former line of above-mentioned made line bolt is placed under the microscope, after measuring the diameter of its end, vertical intercepted length is the former line line segment of line bolt of 21mm, and positive round is processed at the two ends of former for the line bolt obtained line line segment, and the diameter of its diameter and the former thread end of line bolt before processing is consistent;
2.2, bag quilt
Capsulating material obtained in step 1.2 is placed on the glass planar under microscope, then one end of the former line line segment of the line bolt obtained by machining in step 2.1 is elected to be head end, and use microforceps to clamp the former line line segment of line bolt, its head end is embedded in capsulating material, and draw and pull it capsulating material rapidly after stop 3s, can complete the bag quilt to head end, and bag is 5mm by the length of part;
3, wrap by after labelling
3.1, labelling
Step 2.2 is wrapped by after the former line line segment of line bolt be placed in microscope under, slide gauge is used to carry out labelling at the end 12mm of distance bag quilt, and institute makes marks will around the former line line segment of line bolt one week, so as accurately to judge and adjustment inlet wire direction and control the degree of depth of inlet wire, thus make the line bolt after making to enter outside neck smoothly;
3.2, dry
The former line line segment of line bolt through labelling is dried the line bolt finished product that can obtain for mouse MCAO model.
Embodiment 3: a kind of manufacture method of line bolt of mouse MCAO model, by make selection and the preparation of material, head end bag quilt and wrap by after labelling, finally obtain the line bolt being applicable to mouse MCAO model, its concrete manufacture method is as follows:
1, selection and the preparation of material is made
1.1, line bolt material
Polyflon being pulled at 300 DEG C diameter is after the filament of 0.08mm, under 350 DEG C of conditions, obtain in this filament surface the former line making line bolt by titanium dioxide-coated, the natural tension force of the made former line of line bolt is 1.92N, and drawing tension is 1.66 ± 0.9 N;
1.2, the capsulating material of head end
Capsulating material for line bolt head end bag quilt is transparent polymer anti-biotic material, this transparent polymer anti-biotic material be by the silicon emulsion of 15%, 34% polyvinylpyrrolidone, the polyvinyl alcohol of 23%, the Polyethylene Glycol of 16% and 12% politef mix after ultrasonic vibration at 270 DEG C;
2, head end bag quilt
2.1, the processing of the former line of line bolt
The former line of above-mentioned made line bolt is placed under the microscope, after measuring the diameter of its end, vertical intercepted length is the former line line segment of line bolt of 21mm, and positive round is processed at the two ends of former for the line bolt obtained line line segment, and the diameter of its diameter and the former thread end of line bolt before processing is consistent;
2.2, bag quilt
Capsulating material obtained in step 1.2 is placed on the glass planar under microscope, then one end of the former line line segment of the line bolt obtained by machining in step 2.1 is elected to be head end, and use microforceps to clamp the former line line segment of line bolt, its head end is embedded in capsulating material, and draw and pull it capsulating material rapidly after stop 2s, can complete the bag quilt to head end, and bag is 5mm by the length of part;
3, wrap by after labelling
3.1, labelling
Step 2.2 is wrapped by after the former line line segment of line bolt be placed in microscope under, slide gauge is used to carry out labelling at the end 12mm of distance bag quilt, and institute makes marks will around the former line line segment of line bolt one week, so as accurately to judge and adjustment inlet wire direction and control the degree of depth of inlet wire, thus make the line bolt after making to enter outside neck smoothly;
3.2, dry
The former line line segment of line bolt through labelling is dried the line bolt finished product that can obtain for mouse MCAO model.
Because the mouse brain blood vessel diameter of different weight is variant, the line bolt therefore made by the present invention is particularly useful for the mice that body weight is 24 g ± 1.0g.
Line bolt made by the present invention can be immersed in the normal saline containing heparin sodium for subsequent use before use, to reduce the thrombosis around line bolt.
Claims (2)
1. a manufacture method for the line bolt of mouse MCAO model, is characterized in that: this line bolt by the bag of the selection of material and preparation, the former the end of a thread end of line bolt by and wrap by after labelling obtain, its concrete step is as follows:
(1) selection of material and preparation
Polyflon being pulled at 300 DEG C diameter is after the filament of 0.065 ~ 0.1mm, under 350 DEG C of conditions, obtain in this filament surface the former line of line bolt making line bolt by titanium dioxide-coated, then according to weight by the silicon emulsion of 15%, 34% polyvinylpyrrolidone, the polyvinyl alcohol of 23%, the Polyethylene Glycol of 16% and 12% politef at 250 ~ 300 DEG C, after ultrasonic vibration, be mixed to form capsulating material for wrapping quilt;
(2) the bag quilt of the former the end of a thread end of line bolt
First 20 ± 1.0mm is intercepted as the former line line segment of line bolt from the obtained former line of line bolt, positive round is processed at these line segment two ends and is consistent with the diameter of the former thread end of described line bolt, then select wherein one end as the head end of the former line of line bolt, this head end is embedded in the capsulating material obtained by step (1), then the head end of former for line bolt line pull-out capsulating material can be completed the bag quilt to the former the end of a thread end of line bolt;
(3) bag by after labelling
Head end bag by after the former line line segment of line bolt on, the end 11 ~ 12mm of distance bag quilt, carries out labelling in one week around the former line line segment of line bolt, then former for line bolt line line segment is dried the line bolt that can obtain mouse MCAO model.
2. the manufacture method of the line bolt of a kind of mouse MCAO model according to claim 1, it is characterized in that: carrying out in the process of bag quilt to the former the end of a thread end of line bolt, former for line bolt the end of a thread end after capsulating material stops 1 ~ 3s, then is pulled out capsulating material by the former the end of a thread end of line bolt.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510285540.3A CN104887340B (en) | 2015-05-29 | 2015-05-29 | A kind of manufacture method of the line bolt of mouse MCAO model |
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| CN201510285540.3A CN104887340B (en) | 2015-05-29 | 2015-05-29 | A kind of manufacture method of the line bolt of mouse MCAO model |
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| CN104887340B CN104887340B (en) | 2016-09-28 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110524768A (en) * | 2019-09-06 | 2019-12-03 | 长沙迈越生物科技有限公司 | A kind of full automatic line bolt production system and line bolt production method |
| CN110680554A (en) * | 2019-11-18 | 2020-01-14 | 上海市普陀区人民医院 | Experimental animal molding assembly for focal permanent cerebral ischemia |
| CN114259319A (en) * | 2021-12-01 | 2022-04-01 | 北京市神经外科研究所 | A line bolt for making animal cerebral arterial thrombosis model |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110524768A (en) * | 2019-09-06 | 2019-12-03 | 长沙迈越生物科技有限公司 | A kind of full automatic line bolt production system and line bolt production method |
| CN110680554A (en) * | 2019-11-18 | 2020-01-14 | 上海市普陀区人民医院 | Experimental animal molding assembly for focal permanent cerebral ischemia |
| CN114259319A (en) * | 2021-12-01 | 2022-04-01 | 北京市神经外科研究所 | A line bolt for making animal cerebral arterial thrombosis model |
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| CN104887340B (en) | 2016-09-28 |
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