CN201127647Y

CN201127647Y - Nylon thread for focal cerebral ischemia animal model patching method

Info

Publication number: CN201127647Y
Application number: CNU2007200087424U
Authority: CN
Inventors: 李永生
Original assignee: Individual
Current assignee: Individual
Priority date: 2007-11-13
Filing date: 2007-11-13
Publication date: 2008-10-08
Anticipated expiration: 2017-11-13

Abstract

The utility model provides a nylon stopper thread for focal cerebral ischemia animal model suture method, which is formed by connecting a stoppage columnar part with a narrow neck fishing wire. The top end of the stoppage columnar part is in the shape of semi-sphere, and the diameters and the lengths of the stoppage columnar part and the narrow neck fishing wire are different according to the animals with different weights. The prior nylon stopper thread or the nylon stopper thread which is one to two sizes smaller than the prior nylon stopper thread can be used, and the threads with the size of 6 to below zero which can not be used previously and the black and the blue monofilament threads can be used. The nylon stopper thread has simple and reasonable structure, simple production method, low cost, easy use, good operation effect and high success rate.

Description

Focal cerebral ischemia animal model suture method nylon embolus line

Technical field

This utility model belongs to medical science cerebrovascular field, more specifically relates to a kind of focal cerebral ischemia animal model suture method nylon embolus line.

Background technology

Cerebrovascular disease is one of the highest disease of human sickness rate, and often causes patient's deformity.Great changes have taken place at its cause of disease, clinical manifestation, happening part for human cerebral infarction, and its strict pathological tissue, biochemistry and Physiologic Studies often need by the invasive surgical operation, thereby directly win cerebral tissue.And the cerebral ischemia ischemic injuries research to a few minutes in initial several seconds, can only rely on laboratory animal to finish.In addition, ischemia is abnormal hemoperfusion, thereby needs the participation of vascular system, and the tissue of In vitro culture or cell lack this key character.Therefore, the simulation clinical disease is made cerebral ischemia animal model comparatively reliably, its pathogeny of systematic study, pathology, physiology are changed, and curative effect of medication, prophylactico-therapeutic measures etc., all have great importance.

Most of laboratorys select rat as object of study, in recent years, have set up mice focal cerebral ischemia model again.The mouse brain ischemia model is used for the cerebral tissue biochemical metabolism more, estimates the research of curative effect of medication aspect.With respect to its advantage of rat be: be easier to of (1) kind raised more, and price is cheaper; (2) the special type Mus of some requirement of experiment transgenic, this more is easy to get with mice; (3) drug use amount is little; (4) in animal behavior is measured, better expressive ability is arranged; (5) can reduce experiment place, the investment of equipment facility.Shortcoming is because the animal volume is little, with existing existing operation method certain degree of difficulty is arranged, and uses not too extensive.

Existing focal cerebral ischemia model: 1. with Mus anesthesia, lie on the back on operating-table.2. neck medisection skin separates right carotid (CCA), external carotid artery (ECA), internal carotid artery (ICA) successively, irons the bifurcated artery of disconnected ECA, and ligation ECA far-end also irons disconnected.3. close CCA and ICA with the bulldog clamp folder.4. cut an osculum at ECA, inject the heparin sodium normal saline solution with syringe and (during injection, unclamp the bulldog clamp of ICA.After the injection, folder closes once more).5. will install and insert ECA, and with surgical thread firmly ligation gently.6. unclamp the bulldog clamp of ICA, device is taken advantage of a situation insert ICA, to anterior cerebral artery (ACA) initial part, the blood supply of blocking-up middle cerebral artery (MCA).

The shortcoming of existing focal cerebral ischemia model is: on (1) ligation pillow, the thyroid, pharynx liters, tongue, the upper jaw is outer and the certain operation skill of wing corner of the eyes tremulous pulse needs.Operation has changed incidence vascular anatomy structure, and the hemodynamics structure is measured influential to animal behavior.(2) operation is complicated, wound surface is big, hemorrhage many.Blood volume influence to mice is big.Especially to detecting cerebral blood flow, the center blood pressure, influence such as blood parameters is significantly.(3), vessel branch is arranged on the way again, so be difficult for feeling dwell point because route is long.(4) operating time is long, easily exerts an influence because of the stability of anaesthetizing and body temperature changes model.

Existing focal cerebral ischemia model----suture method:

At present its manufacture method is the most popular in the world two kinds for suture method, and a kind of is the Longa method, and another kind is the koizumi method.The former becomes a bead with nylon wire one end scorification, and the latter is coated with one deck silica gel with nylon wire one end.But which kind of method no matter, the diameter of its silk thread front end is difficult for unified.In the Longa method, the silk thread front end is point-to-point with contacting of MCA in addition, does not easily reach intracranial hemorrhage entirely because of animal activity causes infraction.

In suture method, after MCA is blocked the some time, nylon yarn is extracted from ACA, retreat to and recover perfusion again in the ECA.

Summary of the invention

The purpose of this utility model provides a kind of focal cerebral ischemia animal model suture method nylon embolus line; This nylon embolus line structure advantages of simple, preparation method is simple, and is with low cost, easy to use, good operation effect, success rate height.

Focal cerebral ischemia animal model suture method nylon embolus line of the present utility model, this nylon embolus line connects into thin cervical traction line by stopping up cylindrical portion, the top of stopping up cylindrical portion is the hemispherical top, stop up the animal of the diameter of cylindrical portion and thin cervical traction line and length correspondence different weight and different, adopt in the past the nylon embolus line or than in the past little 1 to No. 2 bolt line of nylon embolus line, all can as line body below the 6-0 that can't use or black, blue monofilament lines in the past.

Focal cerebral ischemia animal model suture method of the present utility model with the remarkable advantage of nylon embolus line is:

(1) operation does not change incidence vascular anatomy structure and hemodynamics structure, and is little to the animal behavior shadow;

(2) operation is simple, wound surface is little, go out insufficiency of blood or do not have hemorrhage.Blood volume influence to mice is little.Especially be fit to detect cerebral blood flow, center blood pressure, blood parameters etc.;

(3), easily feel dwell point because route is short;

(4) it is shorter to stay the silk thread of cervical region, can reduce the incidence rate that infraction does not reach intracranial hemorrhage entirely;

(5) operating time short (can finish in three minutes) reduces so anesthesia duration shortening and body temperature are changed, and improves model stability and repeatability;

(6) because of the special shape of its piston-like the end of a thread coated with the hemispherical top, the silk thread front end is point-to-area with contacting of MCA, and infraction reaches fully and is difficult for intracranial hemorrhage takes place, and the success rate of operation is improved.

Description of drawings

Fig. 1 is a nylon embolus line of the present utility model and the photo figure of nylon embolus line in the past, and wherein A is the nylon yarn of common 4-0, and B is the nylon embolus line of Longa method, and C is the nylon embolus line of koizumi method, and D is a nylon embolus skiagram of the present utility model.

Fig. 2 is the structure chart of nylon embolus line of the present utility model.

Fig. 3 is the axonometric chart of nylon embolus line of the present utility model.

Fig. 4 is the vertical view of nylon embolus line of the present utility model.

Fig. 5 is a nylon embolus toe-in composition in the past, and wherein 1 is the 6-0 that can not be shaped, the 7-0 nylon yarn, and 2 is the nylon embolus line (4-0) of Longa method, and head is spherical, and 3 is the nylon embolus line (4-0) of koizumi method, outside irregular.

The specific embodiment

Nylon embolus line preparation process of the present utility model is as follows:

(1) nylon yarn of suitable diameter is penetrated the tubule of regulation bore;

(2) use the interior line end of small power electric flatiron scorification pipe to required length;

(3) extract silk thread out, scorification the end of a thread is to hemispherical, the device product that can obtain to adhere to specification.

Nylon embolus line of the present utility model is used in through internal carotid artery route focal cerebral ischemia animal model---suture method, specifically uses step as follows:

(1) with Mus anesthesia, lies on the back on operating-table the preserved skin sterilization;

(2) neck medisection skin 2CM separates a side common carotid artery (CCA), internal carotid artery (ICA) successively;

(3) 7-0 silk thread ligation and draw the ICA proximal part, the dissecting forceps folder closes the ICA distal end;

(4) on the ICA tube wall at ICA and wing corner of the eyes arterial branch 1mm place, make an osculum with entry needle;

(5) the nylon embolus line is inserted ICA;

(6) unclamp the dissecting forceps that folder closes ICA, the nylon embolus line is pushed through MCA;

(7), wipe out unnecessary nylon embolus line and traction silk thread with the fixing nylon embolus line of silk thread.

After MCA is blocked the some time, nylon yarn is extracted from ICA, simultaneously close the ICA distal end with the dissecting forceps folder, with the fixing osculum of seccotine, remove and draw silk thread, sew up the incision.

Employing is through ICA route focal cerebral ischemia animal model, cooperates nylon embolus line apparatus of the present utility model to use, and only with experimental requirement, selects the suitable thing of inserting, and adopts the shortest path, minimum step, the damage of minimum and reach the purpose of experiment.Reduce the variation of relevant index in the experiment, to avoid producing wrong experimental result.Physiology, biochemistry, morphology, the ethological monitoring of animal nerve had science and advance more.

Claims

1. focal cerebral ischemia animal model suture method nylon embolus line is characterized in that: described nylon embolus line is by stopping up cylindrical portion and thin cervical traction line connects into.

2. focal cerebral ischemia animal model suture method nylon embolus line according to claim 1, it is characterized in that: the top of described obstruction cylindrical portion is the hemispherical top.

3. focal cerebral ischemia animal model suture method nylon embolus line according to claim 1 is characterized in that: the animal of the diameter of the obstruction cylindrical portion of described nylon embolus line and thin cervical traction line and the corresponding different weight of length and different.

4. focal cerebral ischemia animal model suture method nylon embolus line according to claim 1 is characterized in that: described nylon embolus line, adopt in the past nylon embolus line or ratio little 1 to No. 2 bolt line of nylon embolus line in the past.

5. focal cerebral ischemia animal model suture method nylon embolus line according to claim 4 is characterized in that: described nylon embolus line adopts following line body of the 6-0 that in the past can't use or black, blue monofilament lines all can.