CN114250264A - Microbial detection method in moon cake production process and heat preservation box for detection - Google Patents
Microbial detection method in moon cake production process and heat preservation box for detection Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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Abstract
The invention relates to a microorganism detection method in the production process of moon cakes, which samples all the production flows of a single moon cake in a production line, divides the moon cake into two groups, and each group is provided with N objects to be detected; ten times serial dilution is carried out on each substance to be tested of the N substances to be tested in the first group, 5 ml samples are respectively taken from the most suitable three 10 times serial dilutions according to the estimated number, and the number of test tubes growing in each dilution is recorded after culture; dissolving N objects to be detected in the second group to form a liquid to be detected, carrying out gradient dilution on the bacterial liquid to be detected, uniformly mixing a certain volume of the liquid to be detected after each gradient dilution with a proper solid culture medium before solidification, then carrying out heat preservation culture, and recording the culture duration when the colony number exceeds the bacterial standard of the moon cake. The invention realizes the quantitative detection of the bacteria generated in each process, and can determine the disinfection time in each process according to the production cycle of the bacteria to formulate an optimal disinfection scheme.
Description
Technical Field
The invention relates to a microorganism detection method in a moon cake production process and an insulation can for detection, and belongs to the technical field of food detection.
Background
Moon cakes, also called moon cakes, small cakes, rich harvest cakes, round cakes and the like, are one of Chinese traditional gourmet food, have certain requirements on sanitation when making the moon cakes, but the moon cakes are final products of detection and sampling detection at present, and when samples with excessive microorganisms are detected, which flow is problematic cannot be determined, the whole production line needs to be disinfected, and the disinfection period of the production line cannot be determined, so that a detection method capable of determining the flow and disinfecting according to the period is necessary to be designed.
Disclosure of Invention
Aiming at the problems, the invention provides a microorganism detection method in the moon cake production process and an incubator for detection, which can determine which flow has problems according to the detection result and determine the disinfection period of each flow.
In order to achieve the purpose, the invention provides the following technical scheme:
a microorganism detection method in the production process of moon cakes is characterized by comprising the following steps:
1-sampling all the making processes of a single moon cake in a production line at certain time intervals or certain quantity, namely obtaining 2N objects to be tested if N making processes exist; dividing the test object into two groups, wherein each group is provided with N objects to be tested;
2-continuously diluting each substance to be measured of the N substances to be measured in the first group ten times in series, taking 5 ml samples from the most suitable three 10 times of continuous dilution solutions according to the estimated number, inoculating 1 ml to 3 groups of 15 test tubes filled with culture solution, recording the number of test tubes in which each dilution degree grows after culture, then checking a maximum likelihood table MPN to obtain the number of bacteria in the bacteria sample, and calculating the content of viable bacteria in the moon cake according to the sample dilution times;
3-dissolving N objects to be detected in the second group to form a liquid to be detected, carrying out gradient dilution on the bacterial liquid to be detected, uniformly mixing a certain volume of the liquid to be detected after each gradient dilution with a proper solid culture medium before solidification, then carrying out heat preservation culture, taking pictures by a photographing microscope at intervals of 8 hours in the culture process, recording and converting the number of bacterial colonies in a single moon cake according to the pictures, and recording the culture duration when the number of bacterial colonies exceeds the bacterial standard of the moon cake;
4-determining the disinfection interval of each manufacturing flow in the production line according to the culture time.
The invention is further provided with a heat preservation incubator for culture when in heat preservation culture.
The invention is further provided that the culture tray in the heat-preservation incubator is divided into a plurality of placing areas, and cultures are placed in the placing areas.
In the technical scheme, each flow is sampled and divided into two groups, wherein the first group is used for determining the content of viable bacteria in each flow; the second group is used for determining a disinfection period, so that quantitative detection of bacteria generated in each process is realized, disinfection time in each process can be determined according to the production period of the bacteria, and an optimal disinfection scheme is formulated.
The invention also provides an insulation can for detecting microorganisms in moon cakes, which is characterized by comprising a box body and a bearing mechanism arranged in the box body, wherein the bearing mechanism comprises a mounting base, supporting legs, a lifting disc, a first rotating gear and a driving device linked with the lifting disc, the supporting legs are provided with a plurality of parts, one ends of the supporting legs are connected with the mounting base, the other ends of the supporting legs are linked with the bottom of the box body, a stepped seat is arranged in the circumferential direction of the mounting base, the first rotating gear is sleeved on the stepped seat, a through hole is arranged in the center of the mounting base, the lifting disc is lifted and lowered in the through hole through the driving device, the driving device is uniformly distributed with four parts in the circumferential direction of the mounting base, and comprises a second rotating gear, a driving motor, a driving shaft, a first connecting rod and a second connecting rod, the two ends of the driving shaft are respectively connected with the second rotating gear and the first connecting rod, the other end of the first connecting rod is connected with one end of the second connecting rod, the other end of the second connecting rod is connected with the lifting disc, the second rotating gear is meshed with the first rotating gear to rotate, and the top of the heat preservation box is further provided with a photographing microscope.
The lifting box is further provided with an air cylinder at the bottom, and the output end of the air cylinder is abutted against or separated from the bottom of the lifting disc.
The invention further provides that a plurality of placing areas are formed in the lifting disc, and cultures are placed in the placing areas.
Among the above-mentioned technical scheme, when the temperature is cultivateed, can have the difference in temperature in the box to can influence the cultivation effect of culture, thereby influence final result, consequently go up and down the lifting disk with this technical scheme, guarantee the interior unity in each culture temperature of box, and can guarantee the good mobility of bacterium at the lift in-process, improve the accuracy that detects.
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Fig. 1 is a perspective view of an embodiment of the present invention (with a first rotation gear removed).
FIG. 2 is a top view of an embodiment of the present invention.
Fig. 3 is a side view of an embodiment of the present invention.
Fig. 4 is a sectional view of the first rotating gear according to the embodiment of the present invention.
Reference numerals: 10. a box body; 20. arranging a base; 30. supporting legs; 40. a lifting plate; 50. a first rotating gear; 60. a step seat; 70. a through hole; 80. a second rotating gear; 90. a drive motor; 100. a drive shaft; 110. a first link; 120. a second link; 130. a cylinder; 140. and (4) a microscope.
Detailed Description
The following embodiments will be described in detail with reference to the accompanying examples, so that how to implement the technical means for solving the technical problems and achieving the technical effects of the present application can be fully understood and implemented.
The invention relates to a microorganism detection method in the production process of moon cakes, which comprises the following steps:
1-sampling all the making processes of a single moon cake in a production line at certain time intervals or certain quantity, namely obtaining 2N objects to be tested if N making processes exist; dividing the test object into two groups, wherein each group is provided with N objects to be tested;
2-continuously diluting each substance to be measured of the N substances to be measured in the first group ten times in series, taking 5 ml samples from the most suitable three 10 times of continuous dilution solutions according to the estimated number, inoculating 1 ml to 3 groups of 15 test tubes filled with culture solution, recording the number of test tubes in which each dilution degree grows after culture, then checking a maximum likelihood table MPN to obtain the number of bacteria in the bacteria sample, and calculating the content of viable bacteria in the moon cake according to the sample dilution times;
3-dissolving N objects to be detected in the second group to form a liquid to be detected, carrying out gradient dilution on the bacterial liquid to be detected, uniformly mixing a certain volume of the liquid to be detected after each gradient dilution with a proper solid culture medium before solidification, then carrying out heat preservation culture, taking pictures by a photographing microscope at intervals of 8 hours in the culture process, recording and converting the number of bacterial colonies in a single moon cake according to the pictures, and recording the culture duration when the number of bacterial colonies exceeds the bacterial standard of the moon cake;
4-determining the disinfection interval of each manufacturing flow in the production line according to the culture time.
The invention is further provided with a heat preservation incubator for culture when in heat preservation culture.
The invention is further provided that the culture tray in the heat-preservation incubator is divided into a plurality of placing areas, and cultures are placed in the placing areas.
In the technical scheme, each flow is sampled and divided into two groups, wherein the first group is used for determining the content of viable bacteria in each flow; the second group is used for determining a disinfection period, so that quantitative detection of bacteria generated in each process is realized, disinfection time in each process can be determined according to the production period of the bacteria, and an optimal disinfection scheme is formulated.
As shown in fig. 1 to 4, the present invention further provides an incubator for detecting microorganisms in moon cakes, which comprises a box body 10 and a bearing mechanism arranged in the box body 10, wherein the bearing mechanism comprises a placement base 20, supporting legs 30, a lifting disk 40, a first rotating gear 50, and a driving device linked with the lifting disk 40, the supporting legs 30 are provided with a plurality of supporting legs, one end of each supporting leg 30 is connected with the placement base 20, the other end of each supporting leg is linked with the bottom of the box body 10, a stepped seat 60 is arranged in the circumferential direction of the placement base 20, the first rotating gear 50 is sleeved on the stepped seat 60, a through hole 70 is arranged in the center of the placement base 20, the lifting disk 40 is driven by the driving device to ascend and descend in the through hole 70, four driving devices are uniformly distributed in the circumferential direction of the placement base 20, and each driving device comprises a second rotating gear 80, The heat preservation box comprises a driving motor 90, a driving shaft 100, a first connecting rod 110 and a second connecting rod 120, wherein two ends of the driving shaft 100 are respectively connected with the second rotating gear 80 and the first connecting rod 110, the other end of the first connecting rod 110 is connected with one end of the second connecting rod 120, the other end of the second connecting rod 120 is connected with the lifting disc 40, the second rotating gear 80 is meshed with the first rotating gear 50 to rotate, and the top of the heat preservation box is further provided with a photographing microscope 140.
According to the present invention, the bottom of the box 10 is further provided with an air cylinder 130, and an output end of the air cylinder 130 is abutted to or separated from the bottom of the lifting plate 40.
The invention further provides that a plurality of placing areas are formed in the lifting plate 40, and cultures are placed in the placing areas.
Among the above-mentioned technical scheme, when the temperature is cultivateed, can have the difference in temperature in the box 10 to can influence the cultivation effect of culture, thereby influence final result, consequently go up and down lifting disk 40 with this technical scheme, guarantee the unity in each culture temperature in the box 10, and can guarantee the good mobility of bacterium at the lift in-process, improve the accuracy that detects.
The lifting principle is that the driving motor 90 rotates, then the first connecting rod 110 rotates to drive the second connecting rod 120 to do circular motion, and the second connecting rod 120 drives the lifting disc 40 to lift.
In addition, in the description of the embodiments of the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In the description of the present invention, it should be noted that the terms "first", "second", and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Finally, it should be noted that: the above-mentioned embodiments are only specific embodiments of the present invention, which are used for illustrating the technical solutions of the present invention and not for limiting the same, and the protection scope of the present invention is not limited thereto, although the present invention is described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: any person skilled in the art can modify or easily conceive the technical solutions described in the foregoing embodiments or equivalent substitutes for some technical features within the technical scope of the present disclosure; such modifications, changes or substitutions do not depart from the spirit and scope of the embodiments of the present invention, and they should be construed as being included therein. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (6)
1. A microorganism detection method in the production process of moon cakes is characterized by comprising the following steps:
1-sampling all the making processes of a single moon cake in a production line at certain time intervals or certain quantity, namely obtaining 2N objects to be tested if N making processes exist; dividing the test object into two groups, wherein each group is provided with N objects to be tested;
2-continuously diluting each substance to be measured of the N substances to be measured in the first group ten times in series, taking 5 ml samples from the most suitable three 10 times of continuous dilution solutions according to the estimated number, inoculating 1 ml to 3 groups of 15 test tubes filled with culture solution, recording the number of test tubes in which each dilution degree grows after culture, then checking a maximum likelihood table MPN to obtain the number of bacteria in the bacteria sample, and calculating the content of viable bacteria in the moon cake according to the sample dilution times;
3-dissolving N objects to be detected in the second group to form a liquid to be detected, carrying out gradient dilution on the bacterial liquid to be detected, uniformly mixing a certain volume of the liquid to be detected after each gradient dilution with a proper solid culture medium before solidification, then carrying out heat preservation culture, taking pictures by a photographing microscope at intervals of 8 hours in the culture process, recording and converting the number of bacterial colonies in a single moon cake according to the pictures, and recording the culture duration when the number of bacterial colonies exceeds the bacterial standard of the moon cake;
4-determining the disinfection interval of each manufacturing flow in the production line according to the culture time.
2. The method according to claim 1, wherein the incubation culture is performed by placing the moon cake in an incubation incubator.
3. The method according to claim 2, wherein the culture tray in the incubation incubator is divided into a plurality of placement areas, and cultures are placed in the placement areas.
4. An insulation can for detecting microorganisms in moon cakes is characterized by comprising a box body and a bearing mechanism arranged in the box body, wherein the bearing mechanism comprises a placement base, supporting legs, a lifting disc, a first rotating gear and a driving device linked with the lifting disc, the supporting legs are provided with a plurality of supporting legs, one ends of the supporting legs are connected with the placement base, the other ends of the supporting legs are connected with the bottom of the box body, a step seat is arranged in the circumferential direction of the placement base, the first rotating gear is sleeved on the step seat, a through hole is formed in the center of the placement base, the lifting disc is lifted up and down in the through hole through the driving device, the driving device is uniformly distributed with four supporting legs along the circumferential direction of the placement base, the driving device comprises a second rotating gear, a driving motor, a driving shaft, a first connecting rod and a second connecting rod, the two ends of the driving shaft are respectively connected with the second rotating gear and the first connecting rod, the other end of the first connecting rod is connected with one end of the second connecting rod, the other end of the second connecting rod is connected with the lifting disc, the second rotating gear is meshed with the first rotating gear to rotate, and the top of the heat preservation box is further provided with a photographing microscope.
5. The heat preservation box for detecting the microorganisms in the moon cakes according to claim 4, wherein an air cylinder is further arranged at the bottom of the box body, and the output end of the air cylinder is abutted against or separated from the bottom of the lifting disc.
6. The incubator as claimed in claim 5, wherein the elevating plate has a plurality of areas for placing therein the culture.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111596847.7A CN114250264B (en) | 2021-12-24 | 2021-12-24 | Microbial detection method in moon cake production process and heat preservation box for detection |
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| CN202111596847.7A CN114250264B (en) | 2021-12-24 | 2021-12-24 | Microbial detection method in moon cake production process and heat preservation box for detection |
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| CN114250264B CN114250264B (en) | 2022-09-27 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114806846A (en) * | 2022-06-30 | 2022-07-29 | 深圳中食匠心食品有限公司 | Cantonese moon cake detection device and detection method |
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| CN109082360A (en) * | 2017-06-13 | 2018-12-25 | 十堰德远科技开发有限公司 | A kind of laboratory microbial cultivation device |
| CN111735919A (en) * | 2020-07-02 | 2020-10-02 | 浙江莫干山食业有限公司 | Preserved fruit microorganism detection technology |
| CN213060848U (en) * | 2020-06-05 | 2021-04-27 | 邹文兵 | A incubator for microbial detection |
| CN214937392U (en) * | 2021-04-29 | 2021-11-30 | 兰州市妇幼保健院 | Microbial cultivation device |
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- 2021-12-24 CN CN202111596847.7A patent/CN114250264B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109082360A (en) * | 2017-06-13 | 2018-12-25 | 十堰德远科技开发有限公司 | A kind of laboratory microbial cultivation device |
| CN108148729A (en) * | 2018-03-02 | 2018-06-12 | 宁波帝杨电子科技有限公司 | A kind of multifunctional microbial incubator |
| CN213060848U (en) * | 2020-06-05 | 2021-04-27 | 邹文兵 | A incubator for microbial detection |
| CN111735919A (en) * | 2020-07-02 | 2020-10-02 | 浙江莫干山食业有限公司 | Preserved fruit microorganism detection technology |
| CN214937392U (en) * | 2021-04-29 | 2021-11-30 | 兰州市妇幼保健院 | Microbial cultivation device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114806846A (en) * | 2022-06-30 | 2022-07-29 | 深圳中食匠心食品有限公司 | Cantonese moon cake detection device and detection method |
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