CN114806846A - Cantonese moon cake detection device and detection method - Google Patents

Cantonese moon cake detection device and detection method Download PDF

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Publication number
CN114806846A
CN114806846A CN202210754094.6A CN202210754094A CN114806846A CN 114806846 A CN114806846 A CN 114806846A CN 202210754094 A CN202210754094 A CN 202210754094A CN 114806846 A CN114806846 A CN 114806846A
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China
Prior art keywords
moon cake
culture
sampling
tank
detection device
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Granted
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CN202210754094.6A
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Chinese (zh)
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CN114806846B (en
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黄育东
王永来
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Shenzhen Zhongshi Chuangxin Food Co ltd
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Shenzhen Zhongshi Chuangxin Food Co ltd
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Publication of CN114806846A publication Critical patent/CN114806846A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00Means for pre-treatment of biological substances
    • C12M45/04Phase separators; Separation of non fermentable material; Fractionation

Abstract

The invention discloses a Cantonese moon cake detection device and a Cantonese moon cake detection method, which belong to the technical field of food inspection and detection equipment. The displacement device is arranged in the middle of the shell and is in transmission connection with the sampler, and the displacement device is used for driving the sampler to move in a horizontal plane, so that the sampling and the release of the bacterial suspension are facilitated. The sample thief below is provided with constant temperature and cultivates the platform, and constant temperature cultivates the bottom that the platform is located the casing, and constant temperature cultivates the platform constancy of temperature and is used for cultivateing the bacterium to make the bacterium breed fast, convenient observation. The invention has reasonable design and convenient operation, and solves the problem of complicated steps of moon cake sampling and culturing in the prior art.

Description

Cantonese moon cake detection device and detection method
Technical Field
The invention relates to the technical field of food inspection and detection equipment, in particular to a Cantonese moon cake detection device and method.
Background
The moon cake is one of the traditional Chinese cakes which are well known for a long time, the requirement on the quality of the moon cake is higher and higher along with the improvement of the living standard of people, and merchants propose Cantonese moon cakes without food additives such as preservatives, sweetening agents, pigments and the like in order to meet the requirements of people.
And because the moon cake has no preservative, aflatoxin and other toxins are easy to generate to cause harm to the health of consumers, and the moon cake is not favorable for storage, transportation and sale when sold to the outside. Therefore, the method and the device provide a new invention, sample detection is carried out on the moon cake through automatic equipment, and the bacterial content of the moon cake when the moon cake leaves the factory is detected, so that the quality of the moon cake product is effectively controlled.
Disclosure of Invention
Therefore, the invention provides a Cantonese moon cake detection device and a Cantonese moon cake detection method, which aim to solve the problem that in the prior art, the quality guarantee period is difficult to accurately determine because moon cakes do not contain additives.
In order to achieve the purpose, the invention provides the following technical scheme:
according to a first aspect of the present invention,
the invention discloses a Cantonese moon cake detection device which comprises a casing, a dissolving and diluting component, a displacement device, a sampler and a constant-temperature culture platform, wherein the dissolving and diluting component is arranged at the upper part of the casing, the displacement device is arranged in the middle of the casing and is in transmission connection with the sampler, the constant-temperature culture platform is arranged below the sampler, and the constant-temperature culture platform is positioned at the bottom of the casing.
Further, the sample thief includes hollow shaft, step motor, sampling pipe, spring and sampling awl and bores, the last step motor that installs of displacement device, step motor's main shaft is the hollow shaft, the hollow shaft slides and inserts and establish in the sampling awl, the tip of sampling awl and spring offsets, the spring housing is established on the hollow shaft, the hollow shaft is located the axis department of sampling pipe, the sampling pipe sets up on step motor.
Furthermore, the sampling awl drill comprises a liquid outlet plug, an external thread, a drill bit, an inner slot and a sleeve, the hollow shaft is slidably inserted into the sleeve, the hollow shaft is coaxially connected with the sleeve in a transmission manner, the end part of the sleeve is integrally connected with the drill bit, the inner slot is arranged in the drill bit, the liquid outlet plug is slidably arranged in the inner slot, and the external thread is arranged outside the drill bit.
Furthermore, the displacement device comprises a transverse transmission belt, a transverse motor, a longitudinal transmission belt and a longitudinal motor, the stepping motor is arranged on the transverse transmission belt, the transverse transmission belt is sleeved on the transverse motor, the transverse motor is arranged on the longitudinal transmission belt, the longitudinal transmission belt is sleeved on the longitudinal motor, and the longitudinal motor and the stepping motor are both connected with the linear displacement sensor.
Further, dissolve and dilute the subassembly and include centrifuge, storage water tank, dilution box, peristaltic pump, cross valve and three-way valve, the peristaltic pump links to each other with the three-way valve input, two output of three-way valve link to each other with hollow shaft and storage water tank respectively, the storage water tank links to each other with a port of cross valve, the other three port of cross valve links to each other with centrifuge, dilution box and peristaltic pump respectively.
Further, constant temperature cultivation platform includes culture tank, moon cake groove, arrangement board and lift platform, the sampling pipe is located the top of arrangement board, the arrangement board sets up on lift platform, lift platform sets up the bottom at the casing, be provided with moon cake groove and a plurality of culture tank on the arrangement board.
Further, the culture device is arranged in the culture tank, and the electronic scale is arranged in the moon cake tank;
the culture utensil includes culture dish, slide rail, scraper blade, blow down tank and plastic wrap spool, is equipped with the culture dish in the culture tank, the culture dish side is provided with the slide rail, it is provided with the plastic wrap spool to slide in the slide rail, be provided with the scraper blade on the plastic wrap spool, be provided with the blow down tank in the scraper blade.
Further, the casing includes flip, casing, observation window and base, lift platform sets up on the base, the base is located the casing bottom, the casing side is provided with the observation window, the articulated flip that is provided with in casing top.
Furthermore, a heating resistance wire is arranged in the placing plate.
According to a second aspect of the present invention,
the invention discloses a Cantonese moon cake production detection method, which comprises the following steps of applying the Cantonese moon cake detection device;
s1, placing the moon cake sample into a moon cake groove, weighing by using an electronic scale, then moving a sampling pipe to be in contact with the moon cake groove, sampling by using a sampling awl, storing the sample into the sampling pipe, then weighing the rest of moon cakes again, and measuring the weight of the sample;
s2, a peristaltic pump absorbs water from a water storage tank to dissolve a sample, a sample solution is formed and enters a centrifuge through a four-way valve to complete solid-liquid separation to obtain bacterial suspension, the bacterial suspension is input into a sampling pipe through a three-way valve by the peristaltic pump and is injected into a culture apparatus through the sampling pipe, then the bacterial suspension is reversely absorbed into a dilution box from the sampling pipe by the peristaltic pump, then distilled water in the water storage tank is absorbed into the dilution box through the three-way valve by the peristaltic pump, the bacterial suspension in the dilution box is quantitatively diluted, finally the diluted bacterial suspension is put into the culture apparatus, and the bacterial suspension with different concentrations is repeatedly put into different culture apparatuses;
s3, covering the bacteria suspension on the scraper through the discharge groove, coating the bacteria suspension on the bottom of the culture dish, moving the preservative film reel along with the scraper in the coating process, covering the preservative film on the culture dish, and avoiding the invasion of external bacteria.
The invention has the following advantages:
this technical scheme passes through the drill bit sample, and utilize external screw thread and sampling tube tip screw-thread fit to shutoff sampling tube to prevent that solution from leaking, realized the material sample and dissolved the function of material from this on the sample thief, and the availability factor of equipment has been improved, the major structure of equipment has been simplified, secondly, utilize to dissolve and dilute the subassembly, carry out the technical purpose that proportional dilution and ration were distributed after accomplishing solid-liquid separation to the sample of sampling, thereby make the function of equipment more perfect, thereby can effectively detect the bacterial content of moon cake when dispatching from the factory, and carry out the control to the quality of moon cake product.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
The structures, ratios, sizes, and the like shown in the present specification are only used for matching with the contents disclosed in the specification, so that those skilled in the art can understand and read the present invention, and do not limit the conditions for implementing the invention, so that the present invention has no technical essence, and any structural modification, ratio relationship change or size adjustment should still fall within the scope of the technical contents disclosed in the present invention without affecting the function and the achievable purpose of the present invention.
Fig. 1 is a perspective view of a Cantonese moon cake detection device provided by the invention;
FIG. 2 is a cross-sectional view of a culture device of the Cantonese moon cake detection device provided by the invention;
FIG. 3 is a perspective view of a housing of the Cantonese moon cake detection device provided by the invention;
FIG. 4 is a perspective view of a constant temperature culture platform of the Cantonese moon cake detection device provided by the invention;
FIG. 5 is a perspective view of a dissolving and diluting assembly of the Cantonese moon cake detecting device provided by the invention;
FIG. 6 is a perspective view of a sampling awl of the Cantonese moon cake detection device provided by the invention;
fig. 7 is a cross-sectional view of a sampler of the Cantonese moon cake detection device provided by the invention;
fig. 8 is a perspective view of a displacement device of the Cantonese moon cake detection device provided by the invention;
fig. 9 is a mechanism composition block diagram of a Cantonese moon cake detection device provided by the invention;
fig. 10 is a perspective view of the internal structure of the Cantonese moon cake detection device provided by the invention;
in the figure: 1, a machine shell; 11, turning a cover; 12 a housing; 13 an observation window; 14 a base; 2, a dissolving and diluting component; 21, a centrifuge; 22 a water storage tank; 23 a dilution tank; a 24 peristaltic pump; 25 four-way valves; 26 a three-way valve; 3 a displacement device; 31 a transverse conveyor belt; 32 a transverse motor; 33 a longitudinal conveyor belt; 34 a longitudinal motor; 4, a sampler; 41 a hollow shaft; 42 a stepping motor; 43 a sampling tube; a 44 spring; 45 sampling a conical drill; 451 liquid outlet plug; 452 external threads; 453 a drill bit; 454 inner slots; 455 casing pipe; 5, a constant-temperature culture platform; 51 a culture tank; 52 moon cake grooves; 53 placing the plate; 54 a lifting platform; 6 a culture device; 61 a culture dish; 62 a slide rail; 63 a scraper blade; 64 a drain tank; 65 preservative film reel.
Detailed Description
The present invention is described in terms of particular embodiments, and other advantages and benefits of the invention will become apparent to those skilled in the art from the description herein, wherein it is understood that the described embodiments are illustrative of some, but not all, embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
As shown in fig. 1-10, the invention discloses a Cantonese moon cake detection device, which comprises a casing 1, a dissolving and diluting component 2, a displacement device 3, a sampler 4 and a constant temperature culture platform 5, wherein the dissolving and diluting component 2 is arranged at the upper part of the casing 1 and is used for dissolving a sample to form a bacterial suspension, then gradually diluting the bacterial suspension, and respectively putting the bacterial suspension into different culture dishes for culture, thereby comparing bacterial colonies formed by observation, and then determining the number and sanitary condition of bacteria contained in a moon cake. The displacement device 3 is arranged in the middle of the machine shell 1, the displacement device 3 is in transmission connection with the sampler 4, and the displacement device 3 is used for driving the sampler 4 to move in a horizontal plane, as shown in fig. 10, so that the sampling and the release of the bacterial suspension are facilitated. Sampler 4 below is provided with constant temperature and cultivates platform 5, and constant temperature cultivates platform 5 and is located the bottom of casing 1, and 5 temperatures of constant temperature cultivation platform must be used for cultivateing the bacterium to make the bacterium breed fast, convenient observation.
According to an embodiment of the present disclosure, the sampler 4 includes a hollow shaft 41, a stepping motor 42, a sampling tube 43, a spring 44, and a sampling awl 45, as shown in fig. 7, the stepping motor 42 is installed on the displacement device 3, a main shaft of the stepping motor 42 is the hollow shaft 41, so as to drive the hollow shaft 41 to rotate, and a tile on an outer side of the hollow shaft 41 is a prism, because the hollow shaft 41 is slidably inserted into the sampling awl 45, and an inner side of the sampling awl 45 is also a prism, so that when the hollow shaft 41 rotates, the sampling awl 45 can be driven to rotate. The sampling awl 45 is propped against the end part of the spring 44, the spring 44 is sleeved on the hollow shaft 41, the sampling awl 45 is in contact with the moon cake under the action of spring elasticity, the hollow shaft 41 is positioned at the axis of the sampling pipe 43, and when the sampling awl 45 rotates, moon cake chips can be extracted into the sampling pipe 43. Meanwhile, as the hollow shaft 41 is communicated with the peristaltic pump 24 and the sampling pipe 43 is arranged on the stepping motor 42, distilled water in the water storage tank 22 can be introduced into the sampling pipe 43, so that the scraps on the moon cake can be dissolved.
According to one embodiment of the disclosure, the sampling awl 45 comprises a liquid outlet plug 451, an external thread 452, a drill bit 453, an internal insertion groove 454 and a sleeve 455, the hollow shaft 41 is slidably inserted into the sleeve 455, the hollow shaft 41 is in coaxial transmission connection with the sleeve 455 so as to drive the sleeve 455 to rotate together, the end of the sleeve 455 is integrally connected with the drill bit 453, the drill bit 453 is used for drilling moon cake debris, the external thread 452 is arranged outside the drill bit 453, and the internal thread matched with the external thread 452 is arranged at the end of the sampling tube 43, so that when the drill bit 453 rotates continuously, the external thread 452 is matched with the internal thread to block the end of the sampling tube 43, thereby preventing the moon cake debris dissolving liquid from leaking. An inner slot 454 is arranged in the drill bit 453, a liquid outlet plug 451 is arranged in the inner slot 454 in a sliding manner, side holes are arranged on the side surface of the liquid outlet plug 451 and the side surface of the sleeve 455, the end part of the liquid outlet plug 451 leaks outwards, when the liquid outlet plug 451 is contacted with the culture device 6, the liquid outlet plug 451 can be communicated with the sampling tube, and therefore the sample bacterial suspension in the sampling tube is injected into the discharge slot 64.
According to one embodiment of the present disclosure, the displacement device 3 includes a transverse belt 31, a transverse motor 32, a longitudinal belt 33, and a longitudinal motor 34, and the stepping motor 42 is disposed on the transverse belt 31, so that the stepping motor 42 is moved transversely in the X-axis direction by the transverse belt 31. The transverse transmission belt 31 is sleeved on the transverse motor 32 as shown in fig. 7 and 8, so that the whole sampler 4 is driven to move relatively in a belt transmission manner. Similarly, the transverse motor 32 is arranged on the longitudinal transmission belt 33, and the longitudinal transmission belt 33 is sleeved on the longitudinal motor 34, so that the sampler 4 can move to any position within the range of the same horizontal plane through the mutual matching of the transverse transmission belt 31 and the longitudinal transmission belt 33. The longitudinal motor 34 and the stepping motor 42 are connected with linear displacement sensors which are used for transmitting the moving distance between the transverse transmission belt 31 and the longitudinal transmission belt 33, so as to determine the relative position of the sampler 4, and the sampler 4 is controlled by a program preset by an upper computer to complete sampling.
According to one embodiment of the disclosure, the dilution and dissolution assembly 2 includes a centrifuge 21, a water storage tank 22, a dilution tank 23, a peristaltic pump 24, a four-way valve 25 and a three-way valve 26, as shown in fig. 5 and 9, the peristaltic pump 24 is connected to an input end of the three-way valve 26, and the three-way valve 26 is a solenoid valve three-way valve capable of rapidly adjusting a flow direction of the peristaltic pump 24. Wherein, the four-way valve 25 can communicate any two ports of the four ports together. As shown in fig. 9, after the sampling at the sampler 4 is completed, the distilled water in the storage tank 22 passes through the four-way valve 25, the peristaltic pump 24 and the three-way valve 26 in sequence and enters the sampling pipe 43. Two output ends of the three-way valve 26 are respectively connected with the hollow shaft 41 and the water storage tank 22 in an empty mode, and the hollow shaft 41 is communicated with the three-way valve 26. The water storage tank 22 is connected with one port of a four-way valve 25, and the other three ports of the four-way valve 25 are respectively connected with the centrifuge 21, the dilution tank 23 and the peristaltic pump 24;
the specific working process comprises the following steps: when the dissolution of the material in the sampling tube 43 is completed, the peristaltic pump 24 is operated reversely to suck the solution in the sampling tube 43 into the centrifuge 21, the solid-liquid separation is realized, the left bacterial suspension is supplemented into the sampling pipe 43 through the four-way valve 25, the peristaltic pump 24 and the three-way valve 26, at the moment, the sampling tube 43 can put the preliminarily separated bacterial suspension into the constant-temperature culture platform 5 for culture, then, in order to accurately observe the quantity of bacteria contained in the solution, the peristaltic pump 24 reversely pumps the bacterial suspension to enter the dilution tank 23 through the four-way valve 25, at the moment, the water storage tank 22, the three-way valve 26, the peristaltic pump 24, the four-way valve 25 are communicated with the dilution tank 23, distilled water is supplied to the dilution tank 23 by the peristaltic pump 24 to dilute the bacterial suspension, as shown in figure 9, the diluted bacterial suspension is then fed into the culture tanks 51 again, with different culture tanks 51 containing bacterial suspensions of different concentrations for comparison after culture.
According to one embodiment of the present disclosure, as shown in fig. 4, the constant temperature cultivation platform 5 includes a cultivation tank 51, a moon cake tank 52, a placement plate 53, and a lifting platform 54, the sampling tube 43 is located above the placement plate 53, the placement plate 53 is disposed on the lifting platform 54, and the lifting platform 54 can drive the placement plate 53 to float up and down, so that the sampling tube 43 can be put into the bacterial suspension. The lifting platform 54 is arranged at the bottom of the machine shell 1, the placing plate 53 is provided with a moon cake groove 52 and a plurality of culture grooves 51, the moon cake groove 52 is used for placing moon cakes to be detected, and culture dishes 61 are arranged in the culture grooves 51.
According to an embodiment of the present disclosure, the culture apparatus 6 and the electronic scale (not shown in the figure) are further included, the culture apparatus 6 is disposed in the culture tank 51, and the electronic scale is disposed in the moon cake tank 52; before and after sampling, the quality difference of the moon cake groove 52 needs to be measured so as to determine the quality of the sampled moon cake, and the content of bacteria in the sampled moon cake is calculated.
According to one embodiment of the present disclosure, a culture device 6 is shown in FIG. 2. Including culture dish 61, slide rail 62, scraper blade 63, discharge tank 64 and plastic wrap spool 65, culture dish 61 is equipped with in the culture tank 51, and culture dish 61 side is provided with slide rail 62, and it is provided with plastic wrap spool 65 to slide in the slide rail 62, is provided with scraper blade 63 on the plastic wrap spool 65, is provided with discharge tank 64 in the scraper blade 63. As shown in fig. 2, when the bacterial suspension is specifically put in, the bacterial suspension needs to be scraped evenly by the scraper 63 so as to observe bacterial colonies, in actual implementation, the liquid outlet plug 451 is directly inserted into the discharge groove 64, at this time, the liquid outlet plug 451 retracts into the drill 453, so that the bacterial suspension passes through the liquid outlet plug 451 and the uniform flow channel scraper 63 in the discharge groove 64, meanwhile, the sampling tube moves to drive the liquid outlet plug 451 to move, thereby driving the scraper 63 to move, and pulling the preservative film reel 65 while the scraper 63 moves, so that the preservative film covers the culture dish 61, and other bacteria are prevented from invading the culture dish 61.
According to one embodiment of the present disclosure, the housing 1 includes a flip 11, a housing 12, an observation window 13 and a base 14, the lifting platform 54 is disposed on the base 14, the base 14 is located at the bottom of the housing 12, and the observation window 13 is disposed on a side surface of the housing 12 for observing whether the device is operating normally. The top of the shell 12 is hinged with a flip cover 11 to facilitate the maintenance of the dissolving and diluting component 2.
Example 2
The invention discloses a detection method of Cantonese moon cakes, which comprises the following steps of applying the Cantonese moon cake detection device;
s1, placing the moon cake sample into the moon cake groove 52, weighing by using an electronic scale, then moving the sampling pipe 43 to contact with the moon cake groove 52, sampling by using the sampling awl 45, storing the sample into the sampling pipe 43, then weighing the rest of moon cakes again, and measuring the weight of the sample;
s2, the peristaltic pump 24 absorbs water from the water storage tank 22 to dissolve a sample, a sample solution is formed and enters the centrifuge 21 through the four-way valve 25 to complete solid-liquid separation to obtain a bacterial suspension, the bacterial suspension is input into the sampling pipe 43 through the three-way valve 26 by the peristaltic pump 24 and is injected into the culture apparatus 6 through the sampling pipe 43, then the bacterial suspension is reversely sucked into the dilution box 23 from the sampling pipe 43 by the peristaltic pump 24, then the distilled water in the water storage tank 22 is sucked into the dilution box 23 through the three-way valve 26 by the peristaltic pump 24, the bacterial suspension in the dilution box 23 is quantitatively diluted, finally the diluted bacterial suspension is put into the culture apparatus 6, and the bacterial suspension with different concentrations is repeatedly put into different culture apparatuses 6;
s3, covering the bacterial suspension on the scraper 63 through the discharge groove 64, and coating the bacterial suspension on the bottom of the culture dish 61, wherein in the coating process, the preservative film reel 65 moves along with the scraper 63 and covers the preservative film on the culture dish 61 to prevent the invasion of external bacteria.
The application process of the embodiment of the invention is as follows:
the invention discloses a Cantonese moon cake detection device and a detection method, when in specific use, firstly, a moon cake sample is put into a moon cake groove 52 and is weighed by an electronic scale, then a sampling pipe 43 is moved to be in contact with the moon cake groove 52, sampling is carried out through a sampling awl 45, the weight difference is measured, thus obtaining the weight of the sample, then a peristaltic pump 24 absorbs water from a water storage tank 22 to dissolve the sample, a sample solution enters a centrifuge 21 through a four-way valve 25 to complete solid-liquid separation, thus obtaining bacterial suspension, the bacterial suspension is input into the sampling pipe 43 through a three-way valve 26 by the peristaltic pump 24 and is injected into a culture apparatus 6 through the sampling pipe 43, then the bacterial suspension is reversely sucked into a dilution box 23 from the sampling pipe 43 by the peristaltic pump 24, then distilled water in the water storage tank 22 is sucked into the dilution box 23 through the three-way valve 26 by the peristaltic pump 24, the bacterial suspension in the dilution box 23 is quantitatively diluted, and finally, putting the diluted bacterial suspension into the culture device 6, putting the diluted bacterial suspension into different culture devices 6 repeatedly according to different concentrations of bacterial suspension, covering the diluted bacterial suspension onto the scraper 63 through the discharge groove 64, and coating the diluted bacterial suspension at the bottom of the culture dish 61, wherein in the coating process, the preservative film reel 65 moves along with the scraper 63, and covers the preservative film onto the culture dish 61, so that the invasion of external bacteria is avoided.
Although the invention has been described in detail hereinabove with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims (10)

1. The utility model provides a Cantonese moon cake detection device, its characterized in that, includes casing (1), dissolves dilution subassembly (2), displacement device (3), sample thief (4) and constant temperature cultivation platform (5), dissolve dilution subassembly (2) and set up on casing (1) upper portion, the middle part of casing (1) is provided with displacement device (3), displacement device (3) are connected with sample thief (4) transmission, sample thief (4) below is provided with constant temperature cultivation platform (5), constant temperature cultivation platform (5) are located the bottom of casing (1).
2. The Cantonese moon cake detection device according to claim 1, wherein the sampler (4) comprises a hollow shaft (41), a stepping motor (42), a sampling tube (43), a spring (44) and a sampling awl (45), the stepping motor (42) is mounted on the displacement device (3), the main shaft of the stepping motor (42) is the hollow shaft (41), the hollow shaft (41) is slidably inserted into the sampling awl (45), the sampling awl (45) abuts against the end of the spring (44), the spring (44) is sleeved on the hollow shaft (41), the hollow shaft (41) is located at the axis of the sampling tube (43), and the sampling tube (43) is arranged on the stepping motor (42).
3. The Cantonese moon cake detection device according to claim 2, wherein the sampling awl (45) comprises a liquid outlet plug (451), external threads (452), a drill bit (453), an inner insertion groove (454) and a sleeve (455), the hollow shaft (41) is slidably inserted into the sleeve (455), the hollow shaft (41) is in coaxial transmission connection with the sleeve (455), the end of the sleeve (455) is integrally connected with the drill bit (453), an inner insertion groove (454) is formed in the drill bit (453), the liquid outlet plug (451) is slidably arranged in the inner insertion groove (454), and the external threads (452) are arranged outside the drill bit (453).
4. The Cantonese moon cake detection device according to claim 3, wherein the displacement device (3) comprises a transverse transmission belt (31), a transverse motor (32), a longitudinal transmission belt (33) and a longitudinal motor (34), the stepping motor (42) is arranged on the transverse transmission belt (31), the transverse transmission belt (31) is sleeved on the transverse motor (32), the transverse motor (32) is arranged on the longitudinal transmission belt (33), the longitudinal transmission belt (33) is sleeved on the longitudinal motor (34), and the longitudinal motor (34) and the stepping motor (42) are both connected with a linear displacement sensor.
5. The Cantonese moon cake detection device according to claim 3, wherein the dissolving and diluting component (2) comprises a centrifuge (21), a water storage tank (22), a diluting tank (23), a peristaltic pump (24), a four-way valve (25) and a three-way valve (26), the peristaltic pump (24) is connected with an input end of the three-way valve (26), two output ends of the three-way valve (26) are respectively connected with the hollow shaft (41) and the water storage tank (22), the water storage tank (22) is connected with one port of the four-way valve (25), and the other three ports of the four-way valve (25) are respectively connected with the centrifuge (21), the diluting tank (23) and the peristaltic pump (24).
6. The Cantonese moon cake detection device according to claim 4 or 5, wherein the constant-temperature culture platform (5) comprises a culture tank (51), a moon cake tank (52), a placement plate (53) and a lifting platform (54), the sampling pipe (43) is positioned above the placement plate (53), the placement plate (53) is arranged on the lifting platform (54), the lifting platform (54) is arranged at the bottom of the machine shell (1), and the placement plate (53) is provided with the moon cake tank (52) and a plurality of culture tanks (51).
7. The Cantonese moon cake detection device according to claim 6, further comprising a culture utensil (6) and an electronic scale, wherein the culture utensil (6) is arranged in the culture tank (51), and the electronic scale is arranged in the moon cake tank (52);
culture apparatus (6) are equipped with culture dish (61) in culture dish (51) including culture dish (61), slide rail (62), scraper blade (63), discharge tank (64) and plastic wrap spool (65), culture dish (61) in culture dish (51), culture dish (61) side is provided with slide rail (62), it is provided with plastic wrap spool (65) to slide in slide rail (62), be provided with scraper blade (63) on plastic wrap spool (65), be provided with discharge tank (64) in scraper blade (63).
8. The Cantonese moon cake detection device according to claim 6, wherein the casing (1) comprises a flip cover (11), a casing (12), an observation window (13) and a base (14), the lifting platform (54) is arranged on the base (14), the base (14) is positioned at the bottom of the casing (12), the observation window (13) is arranged on the side surface of the casing (12), and the flip cover (11) is hinged to the top of the casing (12).
9. The Cantonese moon cake detection device according to claim 6, wherein a heating resistance wire is arranged in the placement plate (53).
10. A Cantonese moon cake detection method, which comprises the application of the Cantonese moon cake detection device as set forth in any one of claims 1-9, and is characterized by comprising the following steps;
s1, placing the moon cake sample into the moon cake groove (52), weighing by using an electronic scale, then moving the sampling pipe (43) to contact with the moon cake groove (52), sampling by using a sampling awl (45), storing the sample into the sampling pipe (43), weighing the rest of moon cakes again, and measuring the weight of the sample;
s2, a peristaltic pump (24) absorbs water from a water storage tank (22) to dissolve a sample to form a sample solution, the sample solution enters a centrifuge (21) through a four-way valve (25) to complete solid-liquid separation to obtain a bacterial suspension, the bacterial suspension is input into a sampling pipe (43) through a three-way valve (26) by the peristaltic pump (24), the bacterial suspension is injected into a culture apparatus (6) through the sampling pipe (43), then the bacterial suspension is reversely sucked into a dilution box (23) from the sampling pipe (43) by the peristaltic pump (24), then distilled water in the water storage tank (22) is sucked into the dilution box (23) through the three-way valve (26) by the peristaltic pump (24), the bacterial suspension in the dilution box (23) is quantitatively diluted, finally the diluted bacterial suspension is put into the culture apparatus (6), and different culture apparatuses (6) are put in according to the repetition of the bacterial suspensions with different concentrations;
s3, covering the bacterial suspension on the scraper (63) through the discharge groove (64) and coating the bacterial suspension on the bottom of the culture dish (61), wherein in the coating process, the preservative film reel (65) moves along with the scraper (63) and covers the preservative film on the culture dish (61) to prevent external bacteria from invading.
CN202210754094.6A 2022-06-30 2022-06-30 Cantonese moon cake detection device and detection method Active CN114806846B (en)

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