CN114230020A - Treatment method of bamboo pretreatment wastewater - Google Patents
Treatment method of bamboo pretreatment wastewater Download PDFInfo
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- CN114230020A CN114230020A CN202111335962.9A CN202111335962A CN114230020A CN 114230020 A CN114230020 A CN 114230020A CN 202111335962 A CN202111335962 A CN 202111335962A CN 114230020 A CN114230020 A CN 114230020A
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- 239000002351 wastewater Substances 0.000 title claims abstract description 107
- 235000017166 Bambusa arundinacea Nutrition 0.000 title claims abstract description 89
- 235000017491 Bambusa tulda Nutrition 0.000 title claims abstract description 89
- 241001330002 Bambuseae Species 0.000 title claims abstract description 89
- 235000015334 Phyllostachys viridis Nutrition 0.000 title claims abstract description 89
- 239000011425 bamboo Substances 0.000 title claims abstract description 89
- 238000000034 method Methods 0.000 title claims abstract description 38
- 238000000855 fermentation Methods 0.000 claims abstract description 37
- 102000004882 Lipase Human genes 0.000 claims abstract description 36
- 108090001060 Lipase Proteins 0.000 claims abstract description 36
- 239000004367 Lipase Substances 0.000 claims abstract description 35
- 230000004151 fermentation Effects 0.000 claims abstract description 35
- 235000019421 lipase Nutrition 0.000 claims abstract description 35
- 238000003756 stirring Methods 0.000 claims abstract description 33
- 102000004190 Enzymes Human genes 0.000 claims abstract description 30
- 108090000790 Enzymes Proteins 0.000 claims abstract description 30
- 238000002360 preparation method Methods 0.000 claims abstract description 20
- 238000001914 filtration Methods 0.000 claims abstract description 14
- 244000005700 microbiome Species 0.000 claims abstract description 7
- 229940040461 lipase Drugs 0.000 claims description 34
- 229940088598 enzyme Drugs 0.000 claims description 28
- 239000000725 suspension Substances 0.000 claims description 19
- 230000001580 bacterial effect Effects 0.000 claims description 17
- 108010059820 Polygalacturonase Proteins 0.000 claims description 12
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 12
- 108010059892 Cellulase Proteins 0.000 claims description 11
- 229940106157 cellulase Drugs 0.000 claims description 11
- 229940059442 hemicellulase Drugs 0.000 claims description 11
- 108010002430 hemicellulase Proteins 0.000 claims description 11
- 150000001875 compounds Chemical class 0.000 claims description 9
- 241000233866 Fungi Species 0.000 claims description 7
- 239000006228 supernatant Substances 0.000 claims description 7
- 241000948980 Actinobacillus succinogenes Species 0.000 claims description 6
- 241000108664 Nitrobacteria Species 0.000 claims description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 5
- 241000186660 Lactobacillus Species 0.000 claims description 3
- 229940039696 lactobacillus Drugs 0.000 claims description 3
- 229920002488 Hemicellulose Polymers 0.000 abstract description 20
- 239000001913 cellulose Substances 0.000 abstract description 20
- 229920002678 cellulose Polymers 0.000 abstract description 20
- 230000000694 effects Effects 0.000 abstract description 15
- 230000007062 hydrolysis Effects 0.000 abstract description 13
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 13
- 229920005610 lignin Polymers 0.000 abstract description 13
- 239000002253 acid Substances 0.000 abstract description 6
- 238000004065 wastewater treatment Methods 0.000 abstract description 6
- 230000000813 microbial effect Effects 0.000 abstract description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 abstract description 4
- 230000007281 self degradation Effects 0.000 abstract description 4
- 230000020477 pH reduction Effects 0.000 abstract description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 15
- 239000002699 waste material Substances 0.000 description 14
- 239000001814 pectin Substances 0.000 description 13
- 229920001277 pectin Polymers 0.000 description 13
- 235000010987 pectin Nutrition 0.000 description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 11
- 239000008103 glucose Substances 0.000 description 11
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 10
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 10
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 10
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 10
- 230000000593 degrading effect Effects 0.000 description 10
- 230000003301 hydrolyzing effect Effects 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 239000007788 liquid Substances 0.000 description 9
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 8
- IAJILQKETJEXLJ-RSJOWCBRSA-N aldehydo-D-galacturonic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-RSJOWCBRSA-N 0.000 description 8
- 230000003311 flocculating effect Effects 0.000 description 6
- 239000012535 impurity Substances 0.000 description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 238000001556 precipitation Methods 0.000 description 6
- 235000014113 dietary fatty acids Nutrition 0.000 description 5
- 229930195729 fatty acid Natural products 0.000 description 5
- 239000000194 fatty acid Substances 0.000 description 5
- 150000004665 fatty acids Chemical class 0.000 description 5
- 238000005189 flocculation Methods 0.000 description 5
- 230000016615 flocculation Effects 0.000 description 5
- 238000012544 monitoring process Methods 0.000 description 5
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000002378 acidificating effect Effects 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 3
- 239000004310 lactic acid Substances 0.000 description 3
- 235000014655 lactic acid Nutrition 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000010306 acid treatment Methods 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 235000017550 sodium carbonate Nutrition 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 235000011121 sodium hydroxide Nutrition 0.000 description 1
- HUAUNKAZQWMVFY-UHFFFAOYSA-M sodium;oxocalcium;hydroxide Chemical compound [OH-].[Na+].[Ca]=O HUAUNKAZQWMVFY-UHFFFAOYSA-M 0.000 description 1
- 239000001384 succinic acid Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/343—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for digestion of grease, fat, oil
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/26—Nature of the water, waste water, sewage or sludge to be treated from the processing of plants or parts thereof
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Water Supply & Treatment (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Organic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
- Activated Sludge Processes (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a treatment method of bamboo pretreatment wastewater, which comprises the following steps: step 1, adding lipase into bamboo pretreatment wastewater, and stirring for 8-10 hours at the temperature of 40-55 ℃; step 2, continuously adding the complex enzyme preparation, and stirring for 16-48h at the temperature of 30-42 ℃; step 3, placing the bamboo pretreatment wastewater treated in the step 2 into a fermentation container, adding fermentation microorganisms, controlling the temperature to ferment at 22-30 ℃, stirring once every 1-2 hours, detecting the pH value of the wastewater, stopping fermentation when the pH value is less than 6, and standing; and 4, filtering the wastewater treated in the step 3, and separating floccules and supernate. The invention utilizes the functions of self-degradation of cellulose and hemicellulose in the wastewater, biological enzyme hydrolysis and microbial fermentation acidification, naturally acidifies the wastewater and separates out lignin without adding acid, thereby not only reducing the wastewater treatment cost, but also avoiding the negative effects of sulfate radicals, chloride ions and the like on subsequent anaerobic and aerobic procedures.
Description
Technical Field
The invention belongs to the technical field of wastewater treatment, and particularly relates to a treatment method of bamboo pretreatment wastewater.
Background
China is the world with the most abundant bamboo resources, accounts for about one third of the total bamboo resources in the world, and is widely distributed in Fujian, Sichuan, Guangxi, Guangdong, Hunan, Guizhou, Yunnan and other places in China. More than 500 varieties of bamboo are planted, and the planting area, the accumulation and the consumption are the first in the world. According to statistics, the area of the existing bamboo forest in China is more than 420 hectares, the annual cutting amount of the bamboo wood is more than 2500 kilotons, the application of the bamboo wood relates to daily necessities, pharmaceuticals, artware, building industry, textile industry, paper industry and the like, and the annual output value of the bamboo industry exceeds 2000 million yuan.
Bamboo is stiff and compact in structure, and is generally steamed and boiled by an alkaline method in production in order to meet processing requirements, namely, the bamboo is softened by caustic soda, soda ash and lime solution. During the softening process, partial lignin, cellulose, hemicellulose, sugar, fat and other components in the bamboo are dissolved in alkali liquor to form waste water. The waste water is alkaline and has high chemical oxygen content, high treatment difficulty and high cost.
At present, before biochemical treatment, acid treatment is needed to be carried out on the bamboo wastewater, namely, sulfuric acid, hydrochloric acid, acetic acid, oxalic acid and the like are added into the wastewater to separate out lignin in the wastewater, then precipitation removal is carried out, the method for removing the lignin improves the biodegradability of the wastewater, but the difficulty of subsequent treatment is increased, a large amount of acid solution needs to be consumed in the method for acid treatment, the cost of wastewater treatment is increased, sulfate radicals, chloride ions and the like are introduced, and negative effects are easily generated on a subsequent biochemical treatment system.
Disclosure of Invention
The invention aims to: the invention provides a bamboo pretreatment wastewater treatment method, which aims to solve the technical problems that the acid consumption of bamboo wastewater is high, the treatment cost is increased, and the subsequent biochemical treatment is easily influenced.
The technical scheme adopted by the invention is as follows:
a treatment method of bamboo pretreatment wastewater comprises the following steps:
step 1, adding lipase into bamboo pretreatment wastewater, and stirring for 8-10 hours at the temperature of 40-55 ℃;
step 2, continuously adding the complex enzyme preparation, and stirring for 16-48h at the temperature of 30-42 ℃;
step 3, placing the bamboo pretreatment wastewater treated in the step 2 into a fermentation container, adding fermentation microorganisms, controlling the temperature to ferment at 22-30 ℃, stirring once every 1-2 hours, detecting the pH value of the bamboo pretreatment wastewater, stopping fermentation when the pH value is less than 6, and standing;
and 4, filtering the bamboo pretreatment wastewater treated in the step 3, and separating floccules and supernatant.
By adopting the technical scheme, the fat in the bamboo pretreatment wastewater is hydrolyzed by using lipase to generate fatty acid, and the fatty acid neutralizes part of alkali to change the wastewater from alkalescence to alkalescence; continuously adding a complex enzyme preparation to hydrolyze cellulose and hemicellulose in the wastewater into glucose and maltose, hydrolyzing pectin into galacturonic acid, further hydrolyzing the residual fat, simultaneously performing self-fermentation on the cellulose and the hemicellulose to generate part of succinic acid and acetic acid, and further acidifying the wastewater; then carrying out microbial fermentation treatment on the treated wastewater, fermenting glucose and maltose to generate acidic substances such as succinic acid, acetic acid, butyric acid and the like, gradually reducing the pH of the wastewater again, and flocculating and settling impurities such as lignin and the like when the pH is lower than 6; finally separating and removing the floccules to obtain the treated wastewater. The invention utilizes the functions of self-degradation of cellulose and hemicellulose in the wastewater, biological enzyme hydrolysis and microbial fermentation acidification, naturally acidifies the wastewater and separates out lignin without adding acid, thereby not only reducing the wastewater treatment cost, but also avoiding the negative effects of sulfate radicals, chloride ions and the like on subsequent anaerobic and aerobic procedures.
Further, the addition amount of the lipase in the step 1 is 1-5mg per liter of the bamboo pretreatment wastewater.
Further, the adding amount of the complex enzyme preparation in the step 2 is 1-5mg added in each liter of bamboo pretreatment wastewater.
Further, the compound enzyme preparation comprises, by weight, 20-40 parts of cellulase, 40-60 parts of hemicellulase, 5-15 parts of lipase and 5-15 parts of pectinase.
Further, the fermentation microorganism is one or more of lactic acid bacteria, actinobacillus succinogenes, nitrobacteria and white rot fungi.
Further, the fermentation microorganism is prepared into bacterial suspension with the turbidity of 0.8-1.0 by using normal saline, and the bacterial suspension and bamboo pretreatment wastewater are mixed according to the proportion of 1 (1-10) multiplied by 105Is added into the fermentation vessel.
Further, the stirring speed of the step 1 and the step 2 is 100-200r/min, and the stirring speed of the step 3 is 60-100 r/min.
Further, the lipase in the step 1 is alkaline lipase.
Further, the pH value of the bamboo pretreatment wastewater is 10.0-12.0.
Wherein the alkaline lipase is triacylglycerol hydrolase which is commercially available, the enzyme activity of the alkaline lipase is more than or equal to 10 wu/g, the pH application range is 7-11, and the optimal pH is 9.5.
Wherein, the cellulase, hemicellulase, lipase and pectinase in the compound enzyme preparation are all commercially available, the enzyme activity of the cellulase is more than or equal to 20 ten thousand u/g, the pH application range is 7-9, and the optimal pH is 8; the enzyme activity of the hemicellulase is more than or equal to 10 ten thousand u/g, the pH application range is 5-10, and the most suitable pH is 7.5; the enzyme activity of the lipase is more than or equal to 10 ten thousand u/g, the pH application range is 7-11, and the most suitable pH is 9.5; the enzyme activity of the pectinase is more than or equal to 7 wu/g, the pH application range is 6-10, and the most suitable pH is 8.
Wherein, the lactobacillus, the actinobacillus succinogenes, the nitrobacteria and the white rot fungi are all powder and are commercially available.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. the invention utilizes the functions of cellulose and hemicellulose self-degradation, lipase degradation, biological enzyme hydrolysis and microbial fermentation acidification in the bamboo pretreatment wastewater, naturally acidifies the wastewater and separates out lignin without adding acid, thereby not only reducing the wastewater treatment cost, but also avoiding the negative effects of sulfate radicals, chloride ions and the like on subsequent anaerobic and aerobic processes.
2. The method selects commercial lipase with the optimum pH value under the alkaline condition, still has high activity in alkaline wastewater, utilizes the lipase to hydrolyze fat in bamboo pretreatment wastewater to generate fatty acid, the fatty acid neutralizes partial alkali, simultaneously, the self-degradation of each component in the wastewater generates a small amount of acidic substances, so that the wastewater is changed from alkaline to alkalescent, and the pH can be adjusted without adding acid.
3. Cellulose, hemicellulose and pectinase in the wastewater are hydrolyzed into glucose and maltose by cellulase, hemicellulase and pectinase, the glucose and maltose can be used as carbon sources for microbial fermentation, meanwhile, the self-fermentation of the cellulose and the hemicellulose generates partial succinic acid, acetic acid and the like, pectin is hydrolyzed to generate galacturonic acid, and the effect of acidifying the wastewater to a certain degree is achieved; the cellulase group contains xylanase with high activity, cellulose and derivatives thereof in the wastewater can be hydrolyzed into glucose, pectinase can degrade pectin in the wastewater into galacturonic acid, hemicellulase can selectively decompose hemicellulose formed by different types of monosaccharides in the wastewater, and lipase can act on ester bonds of triglyceride to decompose the triglyceride in the wastewater into glycerol and fatty acid; each single enzyme in the compound enzyme preparation has enzyme catalysis specificity, can specifically degrade organic matters, ensures the degradation effect by synergistic use, has stable effect, and enhances the water treatment effect.
4. The degradation products of cellulose and hemicellulose, such as glucose and maltose, are fermented by bacteria such as lactic acid bacteria, actinobacillus succinogenes, nitrobacteria, white rot fungi and the like to generate a large amount of acidic substances such as succinic acid, acetic acid, butyric acid and the like, the pH value of the solution is reduced, when the pH value of the solution is reduced to be below 6, lignin in the waste liquid is flocculated and precipitated, and the removal rate of SS, COD and BOD of the bamboo pretreatment waste water can be effectively improved by filtering and separating.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
A treatment method of bamboo pretreatment wastewater is characterized by comprising the following steps:
(1) degrading fat in the waste liquid:
introducing bamboo pretreatment wastewater with pH value of about 10.0 into a hydrolysis tank, adding alkaline lipase with an addition amount of 1mg/L, stirring at 40-45 deg.C for 8h at a rotation speed of 100 r/min;
(2) hydrolysis of cellulose, hemicellulose, pectin, etc. in the waste liquor:
adding 1mg/L of complex enzyme preparation into the bamboo pretreatment wastewater treated by lipase, stirring for 16h at the temperature of 30-42 ℃ and the rotating speed of 100r/min, hydrolyzing cellulose and hemicellulose in the wastewater into glucose and maltose, and hydrolyzing pectin into galacturonic acid, and further degrading the incompletely treated fat in the step (1); the compound enzyme preparation comprises 20 parts by weight of cellulase, 40 parts by weight of hemicellulase, 5 parts by weight of lipase and 5 parts by weight of pectinase;
(3) flocculation and precipitation by a fermentation method:
placing the bamboo pretreatment wastewater treated in the step (2) in a closed fermentation container, preparing lactobacillus strains into bacterial suspension with the turbidity of 0.8 by using normal saline, wherein the adding amount of the bacterial suspension is 1 multiplied by 105Adding 1 volume of bacterial suspension into bamboo pretreatment wastewater, controlling the temperature to ferment at 22-30 ℃, stirring for 10min every 1h at a stirring speed of 60r/min, monitoring the pH value of the bamboo pretreatment wastewater in real time, stopping fermentation when the pH value is reduced to below 6, standing, and fully flocculating and settling impurities such as lignin and the like;
(4) and (3) filtering:
and (4) filtering the bamboo pretreatment wastewater treated in the step (3), and separating floccules and supernatant to obtain the bamboo pretreatment wastewater solution easy for biochemical treatment.
Example 2
A treatment method of bamboo pretreatment wastewater is characterized by comprising the following steps:
(1) degrading fat in the waste liquid:
introducing the bamboo pretreatment wastewater with pH value of about 11.0 into a hydrolysis tank, adding alkaline lipase with an addition amount of 2mg/L, stirring at 40-45 deg.C for 8.5h at a rotation speed of 120 r/min;
(2) hydrolysis of cellulose, hemicellulose, pectin, etc. in the waste liquor:
adding 2mg/L complex enzyme preparation into the bamboo pretreatment wastewater treated by lipase, stirring for 20h at the temperature of 30-42 ℃ and the rotating speed of 120r/min, hydrolyzing cellulose and hemicellulose in the waste liquid into glucose and maltose, and hydrolyzing pectin into galacturonic acid, and further degrading the incompletely treated fat in the step (1); the compound enzyme preparation comprises 25 parts by weight of cellulase, 45 parts by weight of hemicellulase, 8 parts by weight of lipase and 8 parts by weight of pectinase;
(3) flocculation and precipitation by a fermentation method:
placing the bamboo pretreatment wastewater treated in the step (2) in a closed fermentation container, preparing actinobacillus succinogenes strain into bacterial suspension with the turbidity of 0.85 by using normal saline, wherein the adding amount of the bacterial suspension is 3 multiplied by 105Adding 1 volume of bacterial suspension into bamboo pretreatment wastewater, controlling the temperature to ferment at 22-30 ℃, stirring for 10min every 1h at a stirring speed of 70r/min, monitoring the pH value of the bamboo pretreatment wastewater in real time, stopping fermentation when the pH value is reduced to below 6, standing, and fully flocculating and settling impurities such as lignin and the like;
(4) and (3) filtering:
and (4) filtering the bamboo pretreatment wastewater treated in the step (3), and separating floccules and supernatant to obtain the bamboo pretreatment wastewater solution easy for biochemical treatment.
Example 3
A treatment method of bamboo pretreatment wastewater is characterized by comprising the following steps:
(1) degrading fat in the waste liquid:
introducing bamboo pretreatment wastewater with pH value of about 11.0 into a hydrolysis tank, adding alkaline lipase with the addition amount of 3mg/L, stirring at 45-50 deg.C for 9h at the rotation speed of 150 r/min;
(2) hydrolysis of cellulose, hemicellulose, pectin, etc. in the waste liquor:
adding 3mg/L of complex enzyme preparation into the bamboo pretreatment wastewater treated by lipase, stirring for 30h at the temperature of 30-42 ℃ and the rotating speed of 150r/min, hydrolyzing cellulose and hemicellulose in the waste liquid into glucose and maltose, and hydrolyzing pectin into galacturonic acid, and further degrading the incompletely treated fat in the step (1); the compound enzyme preparation comprises 30 parts by weight of cellulase, 50 parts by weight of hemicellulase, 10 parts by weight of lipase and 10 parts by weight of pectinase;
(3) flocculation and precipitation by a fermentation method:
placing the bamboo pretreatment wastewater treated in the step (2) into a fermentation container, preparing nitrobacteria strain into bacterial suspension with the turbidity of 0.9 in McClure with physiological saline, wherein the addition amount of the bacterial suspension is 5 multiplied by 105Adding 1 volume of bacterial suspension into bamboo pretreatment wastewater, controlling the temperature to ferment at 22-30 ℃, stirring for 10min every 1.5h at a stirring speed of 80r/min, monitoring the pH value of the bamboo pretreatment wastewater in real time, stopping fermentation when the pH value is reduced to below 6, standing, and fully flocculating and settling impurities such as lignin and the like;
(4) and (3) filtering:
and (4) filtering the bamboo pretreatment wastewater treated in the step (3), and separating floccules and supernatant to obtain the bamboo pretreatment wastewater solution easy for biochemical treatment.
Example 4
A treatment method of bamboo pretreatment wastewater is characterized by comprising the following steps:
(1) degrading fat in the waste liquid:
introducing the bamboo pretreatment wastewater with pH value of about 11.0 into a hydrolysis tank, adding alkaline lipase with an addition of 4mg/L, stirring at 45-50 deg.C for 9.5h at a rotation speed of 180 r/min;
(2) hydrolysis of cellulose, hemicellulose, pectin, etc. in the waste liquor:
adding 4mg/L of complex enzyme preparation into the bamboo pretreatment wastewater treated by lipase, stirring for 35h at the temperature of 30-42 ℃ and the rotating speed of 180r/min, hydrolyzing cellulose and hemicellulose in the waste liquid into glucose and maltose, and hydrolyzing pectin into galacturonic acid, and further degrading the incompletely treated fat in the step (1); the compound enzyme preparation comprises 35 parts by weight of cellulase, 55 parts by weight of hemicellulase, 12 parts by weight of lipase and 12 parts by weight of pectinase;
(3) flocculation and precipitation by a fermentation method:
placing the bamboo pretreatment wastewater treated in the step (2) into a fermentation container, preparing white rot fungus strains into fungus suspension with the turbidity of 0.95 in physiological saline, wherein the addition amount of the fungus suspension is 8 multiplied by 10 per minute5Adding 1 volume of bacterial suspension into bamboo pretreatment wastewater, controlling the temperature to ferment at 22-30 ℃, stirring for 10min every 2h at a stirring speed of 90r/min, monitoring the pH value of the bamboo pretreatment wastewater in real time, stopping fermentation when the pH value is reduced to below 6, standing, and fully flocculating and settling impurities such as lignin and the like;
(4) and (3) filtering:
and (4) filtering the bamboo pretreatment wastewater treated in the step (3), and separating floccules and supernatant to obtain the bamboo pretreatment wastewater solution easy for biochemical treatment.
Example 5
A treatment method of bamboo pretreatment wastewater is characterized by comprising the following steps:
(1) degrading fat in the waste liquid:
introducing bamboo pretreatment wastewater with pH value of about 12.0 into a hydrolysis tank, adding alkaline lipase with an addition amount of 5mg/L, and stirring at 50-55 deg.C at a rotation speed of 200r/min for 10 h;
(2) hydrolysis of cellulose, hemicellulose, pectin, etc. in the waste liquor:
adding 5mg/L of complex enzyme preparation into the bamboo pretreatment wastewater treated by lipase, stirring at the temperature of 30-42 ℃ and the rotating speed of 200r/min for 48h to hydrolyze cellulose and hemicellulose in the wastewater into glucose and maltose and pectin into galacturonic acid, and further degrading the incompletely treated fat in the step (1); the compound enzyme preparation comprises 40 parts by weight of cellulase, 60 parts by weight of hemicellulase, 15 parts by weight of lipase and 15 parts by weight of pectinase;
(3) flocculation and precipitation by a fermentation method:
placing the bamboo pretreatment wastewater treated in the step (2) in a closed fermentation container, and mixing lactic acid bacteria and actinobacillus succinogenes according to the proportion of 1: mixing at a weight ratio of 1 to prepare mixed strain, and concocting with normal saline to obtain bacterial suspension with turbidity of 1.0, wherein the addition amount of the bacterial suspension is 1 × 106Adding 1 volume of bacterial suspension into bamboo pretreatment wastewater, controlling the temperature to ferment at 22-30 ℃, stirring for 10min every 2h at a stirring speed of 100r/min, monitoring the pH value of the bamboo pretreatment wastewater in real time, stopping fermentation when the pH value is reduced to below 6, standing, and fully flocculating and settling impurities such as lignin and the like;
(4) and (3) filtering:
and (4) filtering the bamboo pretreatment wastewater treated in the step (3), and separating floccules and supernatant to obtain the bamboo pretreatment wastewater solution easy for biochemical treatment.
Claims (9)
1. A treatment method of bamboo pretreatment wastewater is characterized by comprising the following steps:
step 1, adding lipase into bamboo pretreatment wastewater, and stirring for 8-10 hours at the temperature of 40-55 ℃;
step 2, continuously adding the complex enzyme preparation, and stirring for 16-48h at the temperature of 30-42 ℃;
step 3, placing the bamboo pretreatment wastewater treated in the step 2 into a fermentation container, adding fermentation microorganisms, controlling the temperature to ferment at 22-30 ℃, stirring once every 1-2 hours, detecting the pH value of the bamboo pretreatment wastewater, stopping fermentation when the pH value is less than 6, and standing;
and 4, filtering the bamboo pretreatment wastewater treated in the step 3, and separating floccules and supernatant.
2. The method for treating bamboo pretreatment wastewater as claimed in claim 1, wherein the method comprises the following steps: the addition amount of the lipase in the step 1 is 1-5mg per liter of bamboo pretreatment wastewater.
3. The method for treating bamboo pretreatment wastewater as claimed in claim 1, wherein the method comprises the following steps: the adding amount of the complex enzyme preparation in the step 2 is 1-5mg added in each liter of bamboo pretreatment wastewater.
4. The method for treating bamboo pretreatment wastewater as claimed in claim 3, wherein: the compound enzyme preparation comprises, by weight, 20-40 parts of cellulase, 40-60 parts of hemicellulase, 5-15 parts of lipase and 5-15 parts of pectinase.
5. The method for treating bamboo pretreatment wastewater as claimed in claim 1, wherein the method comprises the following steps: the fermentation microorganism is one or more of lactobacillus, actinobacillus succinogenes, nitrobacteria and white rot fungi.
6. The method for treating bamboo pretreatment wastewater as claimed in claim 5, wherein the method comprises the following steps: the fermentation microorganism is prepared into bacterial suspension with the turbidity of 0.8-1.0 by using normal saline, and the bacterial suspension and bamboo pretreatment wastewater are mixed according to the ratio of 1 (1-10) multiplied by 105Is added into the fermentation vessel.
7. The method for treating bamboo pretreatment wastewater as claimed in claim 1, wherein the method comprises the following steps: the stirring speed of the step 1 and the step 2 is 100-200r/min, and the stirring speed of the step 3 is 60-100 r/min.
8. The method for treating bamboo pretreatment wastewater as claimed in claim 1, wherein the method comprises the following steps: the lipase in the step 1 is alkaline lipase.
9. A method for treating bamboo pretreatment wastewater as claimed in any of claims 1-8, wherein: the pH value of the bamboo pretreatment wastewater is 10.0-12.0.
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| CN116282614A (en) * | 2022-11-03 | 2023-06-23 | 杭州鲲宸环境科技有限公司 | Bamboo product processing wastewater treatment system and treatment method |
| CN116813153A (en) * | 2023-08-25 | 2023-09-29 | 技源生物科技(山东)有限公司 | Method for treating acidic wastewater |
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| CN101250568A (en) * | 2008-04-14 | 2008-08-27 | 大连工业大学 | Method for purifying lignin from paper-making black liquor by fermentation process |
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| CN116813153A (en) * | 2023-08-25 | 2023-09-29 | 技源生物科技(山东)有限公司 | Method for treating acidic wastewater |
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