CN101250568A - Method for purifying lignin from paper-making black liquor by fermentation process - Google Patents
Method for purifying lignin from paper-making black liquor by fermentation process Download PDFInfo
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- CN101250568A CN101250568A CNA2008100110345A CN200810011034A CN101250568A CN 101250568 A CN101250568 A CN 101250568A CN A2008100110345 A CNA2008100110345 A CN A2008100110345A CN 200810011034 A CN200810011034 A CN 200810011034A CN 101250568 A CN101250568 A CN 101250568A
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- 238000000034 method Methods 0.000 title claims abstract description 29
- 238000000855 fermentation Methods 0.000 title claims abstract description 24
- 230000004151 fermentation Effects 0.000 title claims abstract description 23
- 229920005610 lignin Polymers 0.000 title claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 51
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 13
- 239000008103 glucose Substances 0.000 claims abstract description 13
- 238000011218 seed culture Methods 0.000 claims abstract description 10
- 108090000790 Enzymes Proteins 0.000 claims abstract description 9
- 102000004190 Enzymes Human genes 0.000 claims abstract description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 9
- 238000002360 preparation method Methods 0.000 claims abstract description 6
- 235000000346 sugar Nutrition 0.000 claims description 43
- 239000002609 medium Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims description 11
- 238000010564 aerobic fermentation Methods 0.000 claims description 9
- 229940041514 candida albicans extract Drugs 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 8
- 239000012138 yeast extract Substances 0.000 claims description 8
- 238000000926 separation method Methods 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- 108010059820 Polygalacturonase Proteins 0.000 claims description 4
- 241000235070 Saccharomyces Species 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 4
- 235000012204 lemonade/lime carbonate Nutrition 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- 230000003389 potentiating effect Effects 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- 238000009423 ventilation Methods 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 15
- 150000004676 glycans Chemical class 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 229920001282 polysaccharide Polymers 0.000 abstract description 3
- 239000005017 polysaccharide Substances 0.000 abstract description 3
- 238000009776 industrial production Methods 0.000 abstract description 2
- 230000003213 activating effect Effects 0.000 abstract 1
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 230000000593 degrading effect Effects 0.000 abstract 1
- 150000002772 monosaccharides Chemical class 0.000 abstract 1
- 238000000605 extraction Methods 0.000 description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 230000001476 alcoholic effect Effects 0.000 description 5
- 239000011324 bead Substances 0.000 description 5
- 239000012530 fluid Substances 0.000 description 4
- 230000007062 hydrolysis Effects 0.000 description 4
- 238000006460 hydrolysis reaction Methods 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- KJFMBFZCATUALV-UHFFFAOYSA-N phenolphthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2C(=O)O1 KJFMBFZCATUALV-UHFFFAOYSA-N 0.000 description 4
- 150000008163 sugars Chemical class 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 229920002488 Hemicellulose Polymers 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 238000009835 boiling Methods 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-LECHCGJUSA-N alpha-D-xylose Chemical compound O[C@@H]1CO[C@H](O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-LECHCGJUSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000003311 flocculating effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 229960003487 xylose Drugs 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a method for purifying lignin from black liquor through a fermentation method, which comprises following steps: taking alcohol yeast as strains, degrading polysaccharide in the black liquor through first level seed culture, second level seed culture and the fermentation method after activating the strains and through adding enzyme preparation in the fermentation process, disassembling the polysaccharide to be monosaccharide which can be used by the alcohol yeast to grow, thereby glucose in fermentation broth is removed, the purity of the lignin is increased, the viscosity of liquid is lowered along the consumption of the glucose, which is more beneficial for separating lignin subsequently. The strains and the enzyme preparation which are used in the method of the invention are comparatively popular and common in industrial production, and in conclusion, the method of the invention which adopts the fermentation method to extract the lignin from the black liquor has important social benefits, economic benefits and environmental benefits.
Description
Technical field
The present invention relates to the technological process for the treatment of papermaking technical field, specifically is to utilize microorganism to carry out the art breading technology of anaerobism or aerobic fermentation.Also relate to simultaneously the method for extracting a kind of natural high moleculer eompound-xylogen.
Background technology
Xylogen is that nature resultant quantity and amount are only second to cellulosic natural high moleculer eompound.Xylogen constitutes the main body of plant with Mierocrystalline cellulose and hemicellulose, is renewable organic resource the abundantest on the earth.Plant more than 20,000,000,000 tons, is the maximum resource that the mankind can be interdependent by photosynthesis and the renewable xylogen of biochemical action on the annual earth.Especially along with petroleum resources are exhausted day by day, it is particularly important to develop new resource-xylogen.
The stalk that paper making raw material is used, all contain Mierocrystalline cellulose, xylogen and hemicellulose (glycan class) three parts, Mierocrystalline cellulose (accounting for 40%) is wherein only taken in papermaking, and wherein account for things such as 25% xylogen and hemicellulose, wood sugar, potassium, nitrogen, phosphorus, then discarded with black liquor, cause serious environmental to pollute.And, be at present the effective ways that Silvola recovery is handled as black liquid of straw pulp paper-making both at home and abroad.Account angle from resource, the alkali recycling engineering is that multiple Biological resources one torches such as xylogen that have high added value in the black liquor are in a large number come to naught, exchange for indirectly and produce required single boiling alkali, from the ecological engineering angle, this is not a kind of recycling economy technology of the best, still has the inadequate problem of the multi-stage biological utilization of resources.
Therefore, separating lignin and it is applied in paper industry not only can reduce production costs, reclaim useful resources, can also reduce or eliminate the pollution of black liquor to environment, does not reach this purpose effective ways report but still have at present.
Summary of the invention
The present invention adopts fermentation method purifying lignin from black liquid, existing sugar in sugar of carrying secretly along with the increase of floc particle when its objective is and the supernatant liquor except that the delignification flocculating settling, improve the purity of xylogen, and, more help the separation of later stage xylogen along with the consumption of sugar has reduced the viscosity of liquid.
Concrete operation method is as follows:
The preparation of the first step, substratum.
Slant medium, quality group becomes: glucose 1g, yeast extract paste 1g, lime carbonate 1.5g, agar 2.0g, water 100ml.
Seed culture medium, quality group becomes:: glucose 2g, yeast extract paste 1g, peptone 2g, water 100ml, 121 ℃ of high pressure steam sterilization 20～30min.
Fermention medium: black liquid (pH value 11.5), hydrochloric acid debugging pH value is 5.4, the sterilization in the 250ml triangular flask of packing into of 100ml fermention medium.121 ℃ of high pressure steam sterilization 20～30min.
Second step, activated spawn.
Under aseptic condition, with the laboratory with distillery yeast (
Saccharomyces cererisiae) be inoculated on the slant medium, cultivated 3～5 days for 30 ℃, put into refrigerator then and preserve.Stay and do first order seed.
The 3rd step, secondary seed are cultivated.
With first order seed, directly be inoculated in the secondary liquid seed culture medium, under 30 ℃, shaking table was cultivated 24 hours.
The 4th step, fermentation culture.
Use the 250ml triangular flask, every bottle adds the 100ml fermention medium, and adds 0.1% enzyme, every bottle graft kind 10% secondary seed nutrient solution.Wherein said enzyme is comparatively popularized potent prozyme and the polygalacturonase of using always for using in the industry, and its mass ratio is 1: 1.
Fermentation can be used any method in following two kinds of methods:
1, anaerobically fermenting: under 30 ℃, constant temperature culture, per three days mensuration residual sugars.
2, ventilation aerobic fermentation: under 30 ℃, shaking table is cultivated, per 12 hours mensuration residual sugars.
Residual sugar content in the fermented liquid stops fermentation when no longer changing, and measures the spirit yield of fermented liquid and the viscosity of fermented liquid.
The separation of the 5th step, xylogen.
Fermented liquid is through centrifugation, and precipitation lignin residual sugar amount is below 0.3%.
The present invention adopts the xylogen in the fermentation method extraction black liquid, and in black liquid, add degrade polysaccharide in the black liquid of zymin, make it be decomposed into the monose that distillery yeast can be grown and be utilized, thereby remove the sugar in the fermented liquid, improve the purity of xylogen, and, more help the separation of later stage xylogen along with the consumption of sugar has reduced the viscosity of liquid.And distillery yeast has alcohol and produces when anaerobically fermenting, the multi-stage biological resource in the black liquid is fully utilized; And distillery yeast carries out the purer xylogen that obtains that aerobic fermentation can be very fast.Employed bacterial classification and zymin all are comparatively universal and commonly used in the industrial production among the present invention.In a word, the present invention adopts the xylogen in the fermentation method extraction black liquid that important social benefit, economic benefit and environmental benefit are arranged.
Description of drawings
Accompanying drawing 1 width of cloth of the present invention, that is: accompanying drawing 1 is the typical curve of glucose, adopts 3, the fixed sugared method of 5-dinitrosalicylic acid colorimetric is carried out colorimetric estimation when 530nm, be worth knowing the total reducing sugar and the reducing sugar content of sample according to the OD that measures.Wherein X-coordinate is the OD value, and ordinate zou is glucose concn g/L.
Embodiment
The invention will be further described below in conjunction with embodiment.
The preparation of the first step, substratum.
Slant medium: glucose 1g, yeast extract paste 1g, lime carbonate 1.5g, agar 2.0g,
Seed culture medium: glucose 2g, yeast extract paste 1g, peptone 2g, water 100ml, 121 ℃ of high pressure steam sterilization 20～30min.
Fermention medium: black liquid (pH value 11.5), hydrochloric acid debugging pH value is 5.4, the sterilization in the 250ml triangular flask of packing into of 100ml fermention medium.121 ℃ of high pressure steam sterilization 20～30min.
Second step, activated spawn.
Under aseptic condition, with the laboratory with distillery yeast (
Saccharomyces cererisiae) be inoculated on the slant medium, cultivated 3～5 days for 30 ℃, put into refrigerator then and preserve.Stay and do first order seed.
The 3rd step, secondary seed are cultivated.
With first order seed, directly be inoculated in the secondary liquid seed culture medium, under 30 ℃, shaking table was cultivated 24 hours.
The 4th step, fermentation culture.
Use the 250ml triangular flask, every bottle adds the 100ml fermention medium, and (employed enzyme is in the invention: use in the industry and comparatively popularize potent prozyme and the polygalacturonase of using always to add 0.1% enzyme, its mass ratio is 1: 1), every bottle graft kind 10% secondary seed nutrient solution, under 30 ℃, constant temperature culture, per three days mensuration residual sugars.Residual sugar content in the fermented liquid stops fermentation when no longer changing, and measures the spirit yield of fermented liquid and the viscosity of fermented liquid.
One, Tang mensuration
1. the extraction of reducing sugar in the sample: accurately take by weighing the 10ml sample, put into the 100ml beaker, add 85% ethanol 50ml.Mixing, insulation is 30 minutes in 50 ℃ of waters bath with thermostatic control, filters, and filter residue is used 85% extraction using alcohol secondary again.Filtrate is merged, boil off ethanol, add less water, move in the 100ml volumetric flask, be diluted with water to scale, standby.
2. the hydrolysis of total reducing sugar and extraction in the sample: accurately take by weighing the 10ml sample, put into the 100ml beaker, add 10ml 6N hydrochloric acid and 15ml distilled water.Mixing heats about half an hour in boiling water bath, makes the total reducing sugar hydrolysis complete.The cooling back adds phenolphthalein indicator, is neutralized to solution with 10% sodium hydroxide and is blush.Filter and be settled to 100ml, accurately draw above-mentioned solution 10ml again, put into the 100ml volumetric flask, be diluted to scale, standby.
Adopt 3, the fixed sugared method of 5-dinitrosalicylic acid colorimetric is carried out colorimetric estimation when 530nm, according to the OD value of measuring, utilize Fig. 1 to learn the total reducing sugar and the reducing sugar content of sample.
The content of total reducing sugar and reducing sugar in table 1 stoste
| Sample | The OD value | Sugar concentration g/L |
| Reducing sugar | 0.098 | 2.96 |
| Total reducing sugar | 0.084 | 25.51 |
The content of the residual sugar in the fermented liquid of table 2 anaerobically fermenting (seven days)
| Sample | Residual reducing sugar g/L | Residual total reducing sugar g/L |
| Anaerobic fermented liquid | 2.58 | 3.40 |
Two, the mensuration of ethanol content
In the time of about 20 ℃, measure sample 100ml with volumetric flask, all move in the 500ml matrass.With 100ml moisture time washing capacity bottle, washing lotion is incorporated in the matrass, addend grain granulated glass sphere.Load onto prolong, feed cold water, receive distillate (adding ice bath) with former 100ml volumetric flask.Add thermal distillation, when collecting the about 95ml of distillate volume, stop distillation.Be cooled to about 20 ℃ in water-bath, the water constant volume shakes up, and pours in the 100ml graduated cylinder, measures the temperature and the alcoholic strength of distillate.Look into the GB/T13662-2000 appendix A, the alcoholic strength when being converted into 20 ℃ by actual temperature that records and alcoholic strength sign value.
Alcoholic strength and spirit yield in the fermented liquid of table 3 anaerobically fermenting
| Sample | Alcoholic strength (v/v) | Spirit yield g/L |
| Anaerobic fermented liquid | 1.4 | 10.92 |
Three, the mensuration of viscosity
Object is fallen in fluid, the high fluid of viscosity, object falls slow more therein, therefore can compare the size of fluid viscosity from speed of fall.Suppose diameter be d bead viscosity be in the fluid of same substance of η with certain speed ν motion, when satisfying conditions such as speed is very little, ball is rigid ball, and, finally can obtain in the influence of having considered factors such as tube wall
η＝{[d
2(ρ
0-ρ)gt]/(18l)}[1-2.104(d/D)+2.09(d/D)
3]
η-liquid viscosity, P
a.s
ρ
0The density of-bead, K
g/ m
3
ρ-fluidic density, K
g/ m
3
The diameter of d-bead, m
The diameter of D-pipe, m
The run duration of t-bead in liquid, s
The distance that bead falls in the l-time t, m
So, just can be in the hope of viscosity as long as measure the lowering time of certain distance and test portion density.
The viscosity of table 4 stoste and anaerobic fermented liquid
| Sample | Time (s) | Density (K g/m 3) | Viscosity (P a.s) |
| Black liquor | 0.79 | 1075.5 | 8.78 |
| Anaerobic fermented liquid | 0.49 | 1055.5 | 5.45 |
The separation of the 5th step, xylogen.
Fermented liquid is through centrifugation, and precipitation xylogen residual sugar amount is below 0.3%.
Embodiment 2.
The preparation of the first step, substratum.
Slant medium: glucose 1g, yeast extract paste 1g, lime carbonate 1.5g, agar 2.0g,
Seed culture medium: glucose 2g, yeast extract paste 1g, peptone 2g, water 100ml, 121 ℃ of high pressure steam sterilization 20～30min.
Fermention medium: black liquid (pH value 11.5) hydrochloric acid debugging pH value is 5.4, the sterilization in the 250ml triangular flask of packing into of 100ml fermention medium.121 ℃ of high pressure steam sterilization 20～30min.
Second step, activated spawn.
Under aseptic condition, with the laboratory with distillery yeast (
Saccharomyces cererisiae) be inoculated on the slant medium, cultivated 3～5 days for 30 ℃, put into refrigerator then and preserve.Stay and do first order seed.
The 3rd step, secondary seed are cultivated.
With first order seed, directly be inoculated in the secondary liquid seed culture medium, under 30 ℃, shaking table was cultivated 24 hours.
The 4th step, fermentation culture.
Use the 250ml triangular flask, every bottle adds the 100ml fermention medium, and add the enzyme (employed enzyme is in the invention: use in the industry and comparatively popularize potent prozyme and the polygalacturonase of using always) of 0.1g, every bottle graft kind 10% secondary seed nutrient solution, under 30 ℃, shaking table is cultivated, per 12 hours mensuration residual sugars.Residual sugar content in the fermented liquid stops fermentation when no longer changing, and measures the viscosity of fermented liquid.
One, Tang mensuration
1, the extraction of reducing sugar in the sample: accurately take by weighing the 10ml sample, put into the 100ml beaker, add 85% ethanol 50ml.Mixing, insulation is 30 minutes in 50 ℃ of waters bath with thermostatic control, filters, and filter residue is used 85% extraction using alcohol secondary again.Filtrate is merged, boil off ethanol, add less water, move in the 100ml volumetric flask, be diluted with water to scale, standby.
2, the hydrolysis of total reducing sugar and extraction in the sample: accurately take by weighing the 10ml sample, put into the 100ml beaker, add 10ml6N hydrochloric acid and 15ml distilled water.Mixing heats about half an hour in boiling water bath, makes the total reducing sugar hydrolysis complete.The cooling back adds phenolphthalein indicator, is neutralized to solution with 10% sodium hydroxide and is blush.Filter and be settled to 100ml, accurately draw above-mentioned solution 10ml again, put into the 100ml volumetric flask, be diluted to scale, standby.
Adopt 3, the fixed sugared method of 5-dinitrosalicylic acid colorimetric is carried out colorimetric estimation when 530nm, according to the OD value of measuring, utilize the glucose typical curve to learn the total reducing sugar and the reducing sugar content of sample.
The content of the residual sugar in the fermented liquid of table 5 aerobic fermentation (two days)
| Sample | Residual reducing sugar g/L | Residual total reducing sugar g/L |
| Aerobic fermentation liquid | 8.67 | 9.21 |
Two, the mensuration of viscosity
Measure the viscosity of black liquor stoste and aerobic fermentation liquid, result such as table 6 with example 1 viscosimetric with quadrat method:
The viscosity of table 6 stoste and aerobic fermentation liquid
| Sample | Time (s) | Density (K g/m 3) | Viscosity (P a.s) |
| Black liquor | 0.79 | 1075.5 | 8.78 |
| Aerobic fermentation liquid | 0.53 | 1080.5 | 5.89 |
The separation of the 5th step, xylogen.
Fermented liquid is through centrifugation, and precipitation xylogen residual sugar amount is below 0.3%.
Claims (3)
1. the method for fermentation method purifying lignin from black liquid, operate according to the following steps:
The preparation of the first step, substratum
Slant medium: quality group becomes: glucose 1g, and yeast extract paste 1g, lime carbonate 1.5g, agar 2.0g adds water 100ml;
Seed culture medium: quality group becomes: glucose 2g, yeast extract paste 1g, peptone 2g, water 100ml, 121 ℃ of high pressure steam sterilization 20～30min;
Fermention medium: it is 5.4 that black liquid adds hydrochloric acid debugging pH value, the 100ml fermention medium 121 ℃ of high pressure steam sterilization 20～30min in the 250ml triangular flask that pack into;
Second step, activated spawn
Under aseptic condition, with distillery yeast (
Saccharomyces cererisiae) be inoculated on the slant medium, cultivated 3～5 days for 30 ℃, put into refrigerator then and preserve, stay and do first order seed;
The 3rd step, secondary seed are cultivated
With first order seed, directly be inoculated in the secondary liquid seed culture medium, under 30 ℃, shaking table was cultivated 24 hours;
The 4th step, fermentation culture
Use the 250ml triangular flask, every bottle adds the 100ml fermention medium, and adds the enzyme of its quality 0.1%, its quality 10% secondary seed nutrient solution of every bottle graft kind;
Adopt anaerobic fermentation method: in 30 ℃ of following constant temperature culture, measured residual sugar in per 2～3 days, when the residual sugar content in the fermented liquid no longer changes till;
The separation of the 5th step, xylogen;
Fermented liquid is through centrifugation, and precipitation xylogen residual sugar amount is below 0.3%.
2. according to the method for the described a kind of fermentation method of claim 1 purifying lignin from black liquid, it is characterized in that in the fermentation culture in described the 4th step that what adopt is the ventilation aerobic fermentation, its fermentation condition is to cultivate in 30 ℃ of following shaking tables, measured residual sugar in per 12 hours, when the residual sugar content in the fermented liquid no longer changes till.
3. according to the method for the described a kind of fermentation method of claim 1 purifying lignin from black liquid, it is characterized in that described to add zymin in the fermented liquid fermenting process be potent prozyme and polygalacturonase, its mass ratio is 1: 1.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105152458A (en) * | 2015-07-18 | 2015-12-16 | 常州大学 | Method for separating and recovering lignin in paper-making wastewater |
| CN105268461A (en) * | 2015-11-04 | 2016-01-27 | 高大元 | Method for recovering lignin from straw pulp black liquid to prepare lignin carbon carrier catalyst |
| CN106800755A (en) * | 2016-12-27 | 2017-06-06 | 常州市阿曼特化工有限公司 | A kind of method that utilization black liquid prepares high-toughness polylactic acid |
| CN114230020A (en) * | 2021-11-11 | 2022-03-25 | 四川工商职业技术学院 | Treatment method of bamboo pretreatment wastewater |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1105688C (en) * | 1998-12-08 | 2003-04-16 | 中国科学院化工冶金研究所 | Biological acid process for separating lignin from alkaline paper-making black liquor |
| CN100500991C (en) * | 2001-11-13 | 2009-06-17 | 徐守才 | Process for reclaiming and comprehensively utilizing waste digestion liquid of alkaline (or neutral) straw pulp generated by sodium sulfite method |
-
2008
- 2008-04-14 CN CN2008100110345A patent/CN101250568B/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105152458A (en) * | 2015-07-18 | 2015-12-16 | 常州大学 | Method for separating and recovering lignin in paper-making wastewater |
| CN105268461A (en) * | 2015-11-04 | 2016-01-27 | 高大元 | Method for recovering lignin from straw pulp black liquid to prepare lignin carbon carrier catalyst |
| CN106800755A (en) * | 2016-12-27 | 2017-06-06 | 常州市阿曼特化工有限公司 | A kind of method that utilization black liquid prepares high-toughness polylactic acid |
| CN114230020A (en) * | 2021-11-11 | 2022-03-25 | 四川工商职业技术学院 | Treatment method of bamboo pretreatment wastewater |
| CN114230020B (en) * | 2021-11-11 | 2024-05-24 | 四川工商职业技术学院 | Treatment method of bamboo pretreatment wastewater |
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| CN101250568B (en) | 2011-07-20 |
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