CN114158565A - Bactericidal composition containing fenpyrazamine and chlorophenoxyacetic acid amide and application thereof - Google Patents
Bactericidal composition containing fenpyrazamine and chlorophenoxyacetic acid amide and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/16—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof the nitrogen atom being part of a heterocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
Abstract
The invention discloses a bactericidal composition containing fenpyrazamine and chlorobenzene ether amide and application thereof. The composition and the preparation thereof can effectively prevent and treat various fungal diseases, reduce the dosage and improve the prevention and treatment effect.
Description
Technical Field
The invention relates to the technical field of pesticide compounding, and particularly relates to a bactericidal composition containing fenpyrazamine and chlorphenesin amide and application thereof.
Background
Fenpyrazamine, english name: fenpyrazamine, CAS registry number 473798-59-3, chemical name: s-prop-2-alkenyl-5-amino-4- (2-methylphenyl) -3-oxo-2-prop-2-yl pyrazole-1-thiocarbamate is a novel pyridine heterocyclic bactericide developed by Sumitomo chemical Co., Ltd, has a novel chemical structure, can quickly permeate into crops, can quickly exert the pesticide effect on infected fungi, has an action mechanism of inhibiting the biosynthesis of fungal ergosterol, and can inhibit the growth of hyphae and spore germination. The composition is mainly used for preventing and treating Botrytis cinerea (Botrytis cinerea) of grapes, strawberries in the open air and protected crops such as tomatoes, eggplants, hot peppers, cucumbers and pumpkins. In addition, fenpyrazamine is safe to mammals, can be rapidly degraded in the environment, and can be applied before crop harvest.
Chlorobenzene ether amide, chemical name: n- (2- (2, 4-dichlorophenoxy) phenyl) -3- (difluoromethyl) -1-methyl-1H-pyrazole-4-amide, is a novel succinate dehydrogenase inhibitor (SDHIs) compound rapidly discovered by university of China based on the pharmacophore ligation fragment virtual screening strategy (PFVS). The chlorophenoxyacetic acid amide has good control effect on rice sheath blight, potato late blight and rape sclerotinia rot.
Practice proves that the chemical pesticide single agent is easy to have resistance problem after long-term use, so that the dosage is continuously increased, the risk of drug resistance is increased, and the environmental ecological safety is not facilitated. The scientific and reasonable pesticide compounding is sought, which is a better method for solving the problem. The pyraflufen-ethyl and the chlorphenesin are compounded to achieve the synergistic effect, expand the application range of the pyraflufen-ethyl and the chlorphenesin and improve the control efficiency on different germs.
Disclosure of Invention
Based on the situation, the invention aims to provide the bactericidal composition containing the fenpyrazamine and the chlorophenoxyacetic acid amide and the application thereof, and the bactericidal composition is mainly used for preventing and treating fungal diseases of crops, is environment-friendly, safe and effective.
In order to achieve the purpose, the invention provides a bactericidal composition containing fenpyrazamine and chlorobenzene ether amide and application thereof, wherein the bactericidal composition comprises an active ingredient A and an active ingredient B, the active ingredient A is fenpyrazamine, and the active ingredient B is chlorobenzene ether amide;
further, the mass ratio of the fenpyrazamine to the chlorobenzene ether amide is 1: 50-50: 1;
further, the mass ratio of the fenpyrazamine to the chlorobenzene ether amide is 1:50, 1:30, 1:10, 1:5, 1:3, 1:1, 3:1, 5:1, 10:1, 30:1 and 50: 1;
further, the mass ratio of the fenpyrazamine to the chlorobenzene ether amide is 1: 30-10: 1;
further, the mass ratio of the fenpyrazamine to the chlorobenzene ether amide is 1:30, 1:10, 1:5, 1:3, 1:1, 3:1, 5:1 and 10: 1;
further, the mass ratio of the fenpyrazamine to the chlorobenzene ether amide is 1: 10-10: 1;
further, the mass ratio of the fenpyrazamine to the chlorobenzene ether amide is 1:10, 1:5, 1:3, 1:1, 3:1, 5:1 and 10: 1;
further, the mass ratio of the fenpyrazamine to the chlorobenzene ether amide is 1: 5-5: 1;
further, the mass ratio of the fenpyrazamine to the chlorobenzene ether amide is 1:5, 1:3, 1:1, 3:1 and 5: 1;
further, the mass ratio of the fenpyrazamine to the chlorobenzene ether amide is 1:3, 1:1 and 3: 1;
further, the total weight of the bactericidal composition is 100 wt%, and the content of the active ingredients in the bactericidal composition is 1-80 wt%;
further, the total weight of the bactericidal composition is 100 wt%, and the content of the active ingredients in the bactericidal composition is 5-60 wt%.
The bactericidal composition of the present invention can be prepared by a common processing method known to those skilled in the art, that is, after mixing the active substance with a liquid solvent or a solid carrier, one or more surfactants such as wetting agent, dispersant, emulsifier, thickener, disintegrant, antifreeze, defoamer, solvent, stabilizer, etc. are added;
further, the wetting agent is selected from one or a mixture of more of sodium dodecyl benzene sulfate, sodium dodecyl benzene sulfonate, saponin powder, alkyl sulfate, nekal BX, silkworm excrement, alkylphenol ethoxylates, fatty alcohol-polyoxyethylene ether, alkanolamide-polyoxyethylene ether and phosphate or sulfate thereof, and alkyl polyoxyethylene ether succinate sulfonate;
further, the dispersing agent is selected from one or a mixture of a plurality of polycarboxylate, lignosulfonate, naphthalene or alkyl naphthalene formaldehyde condensate sulfonate, calcium alkylbenzene sulfonate, alkylphenol polyoxyethylene phosphate, fatty alcohol polyoxyethylene polyoxypropylene ether, fatty alcohol polyoxypropylene polyoxyethylene ether and polymeric alkyl aryl sulfonate;
further, the emulsifier is selected from one or more of agricultural milk 500# (calcium alkyl benzene sulfonate), Ningru 36# (phenethylphenol formaldehyde resin polyoxyethylene ether), agricultural milk 400# (benzyldimethylphenol polyoxyethylene ether), agricultural milk 700# (alkylphenol formaldehyde resin polyoxyethylene ether), agricultural milk 1600# (phenethylphenol polyoxyethylene polypropylene ether), OP series (nonylphenol polyoxyethylene ether), BY series (castor oil polyoxyethylene ether), agricultural milk 33# (alkylaryl polyoxyethylene polyoxypropylene ether), span series (sorbitan monostearate), Tween series (sorbitan fatty acid ester polyoxyethylene ether) or AEO series (fatty alcohol polyoxyethylene ether);
further, the thickening agent is selected from one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose or magnesium aluminum silicate;
further, the disintegrating agent is selected from one or more of bentonite, urea, ammonium sulfate, sodium chloride, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate;
further, the antifreezing agent is a mixture consisting of one or more of alcohols, alcohol ethers or inorganic salts;
further, the defoaming agent is selected from one or a mixture of silicone oil and silicone compounds;
further, the solvent is selected from one or a mixture of more of N, N-dimethylformamide, cyclohexanone, benzene, toluene, xylene, dimethyl sulfoxide, methanol, ethanol, trimethylcyclohexanone, N-methylpyrrolidone, N-octylpyrrolidone, triethanolamine, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, propanol, butanol, butyl ether, ethanolamine, isopropylamine, ethyl acetate, acetonitrile, mineral spirits, vegetable oils, vegetable oil derivatives or deionized water;
further, the stabilizing agent is selected from one or more of epoxidized soybean oil, epichlorohydrin, BHT, oxalic acid, succinic acid, adipic acid, ethyl acetate and triphenyl phosphate;
further, the penetrating agent is selected from one or more of a penetrating agent JFC (fatty alcohol-polyoxyethylene ether), a penetrating agent T (diisooctyl maleate sulfonate), azone or organic silicon;
further, the carrier is one, two or three of a solvent or a filler, and water is preferably deionized water;
further, the filler is selected from one or a mixture of more of kaolin, diatomite, bentonite, attapulgite, white carbon black, starch or light calcium carbonate;
all of the above are commercially available.
Further, the bactericidal composition can be prepared into any preparation form acceptable in agriculture;
further, the preparation formulation is selected from any one of powder, granules, soluble powder, soluble granules, soluble tablets, water dispersible granules, wettable powder, water dispersible tablets, microcapsule suspending agents, dispersible agents, missible oil, emulsion in water, microemulsion, suspending agents, suspoemulsion and soluble agents;
furthermore, the preparation formulation can be any one of suspending agent, microemulsion, missible oil, water dispersible granule and wettable powder.
The invention provides an application of a bactericidal composition in preventing and treating fungal diseases of agricultural plants;
further, the fungal diseases are one or more of gray mold, sclerotinia sclerotiorum, sheath blight and late blight;
furthermore, the fungal diseases are gray mold and sclerotinia sclerotiorum;
further, the plant is a protected or field crop.
The technical scheme of the invention has the beneficial effects that:
1) the bactericidal composition has obvious synergistic effect on the prevention and treatment effects of various fungal diseases, and the prevention and treatment effects are obviously improved;
2) the usage amount and the application times of the pesticide are reduced, the prevention and treatment cost is reduced, and the environment-friendly effect is achieved;
3) the two active components are mixed to effectively control pathogenic microorganisms to generate drug resistance, and the service life of the medicament can be prolonged.
Detailed Description
Preparation example 1: 30% of fenpyrazamine-chlorophenoxy ether amide suspension (1:3) (7.5% + 22.5%)
According to the weight percentage, fenpyrazamine 7.5%, chlorobenzene ether amide 22.5%, fatty alcohol-polyoxyethylene ether 1%, styryl phenol-polyoxyethylene ether phosphate 3%, sodium polycarboxylate 1%, alkylaryl polyoxyethylene polyoxypropylene ether 2%, magnesium aluminum silicate 1%, xanthan gum 0.2%, sodium sorbate 0.5%, silicone oil 0.5%, ethylene glycol 5%, and deionized water for the balance.
The preparation method comprises the following steps: uniformly mixing water, a dispersing agent and a wetting agent, sequentially adding a defoaming agent and an antifreezing agent under a stirring state, uniformly mixing through high-speed shearing, adding fenpyrazamine and chlorobenzene ether amide, continuously uniformly mixing through shearing, grinding in a horizontal sand mill, controlling the particle size of the material to be below 5 mu m, finally adding a thickening agent, and uniformly shearing to obtain the suspending agent.
Preparation example 2: 20% Aminopyrafen-chlorophenyl Ether amide suspension (3:1) (15% + 5%)
According to the weight percentage, fenpyrazamine 15%, chlorobenzene ether amide 5%, isotridecanol polyoxyethylene ether 1%, polycarboxylic acid sodium salt 1%, alkylaryl polyoxyethylene polyoxypropylene ether 2%, alkylphenol polyoxyethylene ether phosphate 3%, silicone oil 0.4%, magnesium aluminum silicate 1%, isothiazolinone 0.1%, xanthan gum 0.3%, propylene glycol 5%, and deionized water for the balance.
The preparation method comprises the following steps: the same as in preparation example 1.
Preparation example 3: 3% Aminopyrafen-chlorophenyl Ether amide microemulsion (1:1) (1.5% + 1.5%)
The composition comprises, by weight, 1.5% of fenpyrazamine, 1.5% of chlorophenoxy amide, 15% of xylene, 20% of cyclohexanone, 15% of styryl benzene polyoxyethylene ether, 3% of EO-PO block copolymer, 1% of fatty alcohol polyoxyethylene ether sodium sulfate, 4% of glycerol, 0.05% of an organic silicon defoamer and the balance of deionized water.
The preparation method comprises the following steps: uniformly mixing the active ingredient fenpyrazamine with chlorophenoxy acetic acid amide, a solvent, an emulsifier and the like to prepare an oil phase, uniformly mixing an antifreezing solution with water to prepare a water phase, adding the oil phase into the water phase under the stirring state, uniformly stirring, continuously shearing for 10min, adding a silicone oil antifoaming agent, and uniformly stirring to obtain small liquid drops with the oil phase particles of 0.01-0.1 micron, thus preparing the microemulsion.
Preparation example 4: 30% Aminopyrafen-chlorphenesin wettable powder (5:1) (25% + 5%)
The composition comprises, by weight, 25% of fenpyrazamine, 5% of chlorobenzene ether amide, 7% of sodium lignosulfonate, 4% of dispersant NNO, 3% of sodium dodecyl sulfate and the balance kaolin.
The preparation method comprises the following steps: mixing the active ingredient fenpyrazamine, the chlorophenoxyacetic acid amide, the dispersant, the wetting agent and the filler, uniformly stirring in a stirring kettle, and repeatedly crushing and uniformly mixing through a jet mill to prepare the wettable powder of the composition.
Preparation example 5: 20% fenpyrazamine-chlorobenzene ether amide missible oil (3:2) (12% + 8%)
According to the weight percentage, fenpyrazamine 12%, chlorobenzene ether amide 8%, N, N-dimethylformamide 10%, cyclohexanone 30%, styrylphenol polyoxyethylene ether 13.5%, calcium dodecyl benzene sulfonate 1.5%, and xylene to make up the balance.
The preparation method comprises the following steps: dissolving active ingredients of fenpyrazamine and chlorobenzene ether amide in an organic solvent according to a formula proportion, adding a certain amount of emulsifier, and stirring and mixing to prepare a homogeneous transparent oily liquid.
Preparation example 6: 24% fenpyrazamine-chlorophenoxylate amide water dispersible granule (1:5) (4% + 20%)
The composition comprises, by weight, 4% of fenpyrazamine, 20% of chlorobenzene ether amide, 8% of naphthalenesulfonate formaldehyde condensate, 2% of sodium polycarboxylate, 2% of sodium dodecyl sulfate, 5% of white carbon black and the balance of kaolin.
The preparation method comprises the following steps: adding active ingredients of fenpyrazamine and chlorobenzene ether amide into a carrier, adding a surfactant and other functional auxiliaries, mixing, performing jet milling, adding 10-25% of water, kneading, granulating, drying and screening to obtain the water dispersible granule product.
Indoor activity test:
example 1 Activity measurement of Aminopyrafen and Chlorobenzene Ether amide in the Chamber of Botrytis
The test basis is as follows: the test refers to NY/T1156.6-2006 "indoor bioassay pesticide test criteria fungicide part 6: combined action assay for compounding, NY/T1156.2-2006 "indoor bioassay of pesticides test criteria fungicide part 2: the plate method of the experiment for inhibiting the growth of pathogenic bacteria hypha.
Test targets: botrytis cinerea (Botrytis cinerea), a strain that is continuously cultured by the research and development center of the group.
Test drugs: 95% of chlorphenesin amide raw drug and 97% of fenpyrazamine raw drug, and the experimental drugs are provided by group research and development centers.
Preparing a medicament: dissolving the raw medicine with DMF, and diluting with 0.1% Tween-80 water solution to obtain 5 series of mass concentrations.
The test method comprises the following steps:
adding the dissolved treatment liquid medicines into a PDA culture medium which is melted and cooled to about 35 ℃ in proportion, fully shaking up, pouring into a sterilized culture dish (diameter is 90mm) to prepare a drug-carrying flat plate, repeating the treatment for 4 times, and treating with a sterilized 0.1% Tween-80 aqueous solution as a blank control; and (3) selecting the cultured pathogenic bacteria cake by using a sterilized puncher (with the diameter of 5mm), inoculating the pathogenic bacteria cake to the center of a flat plate with the bacteria in a sterile environment, placing the flat plate in a constant-temperature incubator at 25 ℃ for culture, and vertically measuring the diameter of each bacterial colony by adopting a cross method after culturing for 5 days.
The calculation method comprises the following steps: the inhibition rate of the hyphal growth of the test target bacteria by each treatment concentration was calculated from the results of the investigation according to the following formula and expressed in percentage (%).
D＝D1-D2
In the formula:
d-colony growing diameter;
D1-colony diameter;
D2-the diameter of the cake.
I-hypha growth inhibition rate;
D0-growing the diameter of the blank colony;
Dtmedicament-treated colonies grow in diameter.
And (3) test statistics: and processing the data by adopting a method of analyzing a few rate value. Analyzing by IBM SPSS Statistics 20 statistical analysis system to obtain virulence regression line and EC50Value and correlation coefficient R2The activity of the test agent on the biological sample is evaluated.
The co-toxicity coefficient (CTC value) of the blend was calculated by the following equation:
in the formula:
ATI-actually measured toxicity index of mixed agent;
S-EC of Standard Fungicide50In milligrams per liter (mg/L);
EC of M-mixtures50In milligrams per liter (mg/L).
TTI＝TIA*PA+TIB*PB
In the formula:
TTI-mixture theory virulence index;
TIA-agent virulence index a;
PAthe percentage content of the A medicament in the mixture is hundredFraction (%);
TIB-agent B virulence index;
PB-the percentage of the agent B in the mixture in percent (%).
In the formula:
CTC-co-toxicity coefficient;
ATI-actually measured toxicity index of mixed agent;
TTI-mixture theory virulence index.
The compounded co-toxicity coefficient CTC is more than or equal to 120, and the synergistic effect is shown; CTC is less than or equal to 80 and shows antagonism; 80 < CTC < 120 showed additive effects.
The test results are shown in Table 1.
TABLE 1 measurement results of the joint toxicity of Aminopyridone and Chlorobenzene ether amide mixed pairing Botrytis cinerea at different ratios
| Test reagent and proportion | Regression equation | Coefficient of correlation R2 | EC50(mg/L) | Co-toxicity coefficient |
| Aminopyrafen A | y＝2.422x-0.563 | 0.997 | 1.708 | - |
| Chlorophenylene ether amide B | y＝2.571x-0.449 | 0.993 | 1.495 | - |
| A:B＝1:50 | y＝1.831x-0.180 | 0.984 | 1.254 | 119.511 |
| A:B＝1:30 | y＝1.504x-0.081 | 0.991 | 1.132 | 132.601 |
| A:B＝1:10 | y＝1.566x+0.101 | 0.988 | 0.862 | 175.423 |
| A:B＝1:5 | y＝1.455x+0.166 | 0.996 | 0.769 | 198.535 |
| A:B＝1:3 | y＝1.498x+0.249 | 0.992 | 0.682 | 226.262 |
| A:B＝1:1 | y＝1.413x+0.124 | 0.990 | 0.817 | 195.155 |
| A:B＝3:1 | y＝1.723x+0.003 | 0.983 | 0.996 | 165.588 |
| A:B＝5:1 | y＝1.439x-0.059 | 0.991 | 1.099 | 151.809 |
| A:B＝10:1 | y＝1.835x-0.220 | 0.987 | 1.318 | 127.933 |
| A:B＝30:1 | y＝1.848x-0.347 | 0.998 | 1.541 | 110.330 |
| A:B＝50:1 | y＝1.848x-0.423 | 0.998 | 1.694 | 100.546 |
As can be seen from Table 1, the chlorophenoxylate amide has high toxicity to cucumber botrytis cinerea, EC50The weight ratio of the fenpyrazamine to the chlorophenyl ether amide is 1.495mg/L, the weight ratio of the fenpyrazamine to the chlorophenyl ether amide is in the range of 1: 50-50: 1, and the co-toxicity coefficients are all larger than 80, which indicates that the blending of the fenpyrazamine and the chlorophenyl ether amide in the range shows additive or synergistic effect, and no antagonistic effect occurs.
When the weight ratio of the fenpyrazamine to the chlorobenzene ether amide is 1: 30-10: 1, the co-toxicity coefficients are all larger than 120, and the two show synergistic action within the weight ratio range; when the weight ratio of the fenpyrazamine to the chlorobenzene ether amide is 1: 10-5: 1, the cotoxicity coefficients are both larger than 150, and the synergistic effect of the fenpyrazamine and the chlorobenzene ether amide is remarkable in the weight range; wherein, when the weight ratio of the fenpyrazamine to the chlorobenzene ether amide is 1:3, the co-toxicity coefficient is 226.262, and the synergistic effect is most remarkable.
Example 2 Activity assay of Aminopyrafen and Chlorobenzene Ether amide in-house Activity of Sclerotinia sclerotiorum
The test basis is as follows: the test refers to NY/T1156.6-2006 "indoor bioassay pesticide test criteria fungicide part 6: combined action assay for compounding, NY/T1156.2-2006 "indoor bioassay of pesticides test criteria fungicide part 2: the plate method of the experiment for inhibiting the growth of pathogenic bacteria hypha.
Test targets: sclerotinia sclerotiorum (sclerotitiorum) is a strain continuously cultured by the research and development center of the group.
Test drugs: 95% of chlorphenesin amide raw drug and 97% of fenpyrazamine raw drug, and the experimental drugs are provided by group research and development centers.
Preparing a medicament: dissolving the raw medicine with DMF, and diluting with 0.1% Tween-80 water solution to obtain 5 series of mass concentrations.
The test method comprises the following steps:
adding the dissolved liquid medicine into melted PDA culture medium cooled to about 35 deg.C, shaking thoroughly, pouring into sterilized culture dish (diameter 90mm) to make into drug-carrying plate, repeating for 4 times, and processing with sterilized 0.1% Tween-80 water solution as blank control; picking out the sclerotinia sclerotiorum cake of rape by a sterilized puncher (diameter is 5mm), inoculating the sclerotinia sclerotiorum cake of rape to the center of a flat plate with a medicine in a sterile environment, placing the flat plate in a constant-temperature incubator at 25 ℃ for culture, and measuring the diameter of a bacterial colony by a cross method after culturing for 3 days.
And (3) test statistics: and processing the data by adopting a method of analyzing a few rate value. Can be analyzed by an IBM SPSS Statistics 20 statistical analysis system to obtain a virulence regression line and EC50Value and correlation coefficient R2The activity of the test agent on the biological sample is evaluated.
The formula for calculating the co-toxicity coefficient (CTC value) of the mixture is the same as that in example 1.
The test results are shown in Table 2.
TABLE 2 measurement results of the joint toxicity of the pyriminostrobin and the chlorophenoxyacetic acid amide mixed pairing sclerotinia sclerotiorum in different proportions
| Test reagent and proportion | Regression equation | Coefficient of correlation R2 | EC50(mg/L) | Co-toxicity coefficient |
| Aminopyrafen A | y＝2.979x-1.537 | 0.986 | 3.280 | - |
| Chlorophenylene ether amide B | y＝3.814x-2.933 | 0.996 | 5.874 | - |
| A:B＝1:10 | y＝2.612x-1.715 | 0.993 | 4.535 | 120.838 |
| A:B＝1:5 | y＝2.859x-1.584 | 0.987 | 3.581 | 144.929 |
| A:B＝1:3 | y＝2.543x-1.189 | 0.995 | 2.935 | 167.099 |
| A:B＝1:1 | y＝2.436x-0.808 | 0.985 | 2.146 | 196.154 |
| A:B＝3:1 | y＝2.954x-0.976 | 0.987 | 2.140 | 172.292 |
| A:B＝5:1 | y＝2.437x-0.847 | 0.989 | 2.226 | 159.056 |
| A:B＝10:1 | y＝2.686x-1.124 | 0.991 | 2.621 | 130.377 |
As can be seen from Table 2, fenpyrazamine has a high virulence index for Sclerotinia sclerotiorum and EC503.280mg/L, when the weight ratio of the fenpyrazamine to the chlorobenzene ether amide is 1: 10-10: 1, the co-toxicity coefficients are all larger than 120, and the weight ratio range of the fenpyrazamine to the chlorobenzene ether amide shows a synergistic effect; when the weight ratio of the fenpyrazamine to the chlorobenzene ether amide is in the range of 1: 5-5: 1, the cotoxicity coefficients are all larger than 140, the synergistic effect is obvious, wherein when the weight ratio of the fenpyrazamine to the chlorobenzene ether amide is 1:1, the cotoxicity coefficient is the largest and is 196.154, and the synergistic effect is the most obvious.
And (3) field efficacy test:
example 3 Aminopyrafen and Chlorobenzene Ether amide mix-pair cucumber Gray mold field efficacy test
The test basis is as follows: the test refers to GB/T17980.28-2000 'test criterion for pesticide effect in field for preventing and treating gray mold of vegetables'.
Test site: in the vegetable planting base in shou city, Shandong province, the test land is used for protected land cultivation, the organic matter content of the soil of the test land is high, the field management is good, and the cultivation conditions of all test districts are consistent. The disease condition of the test site occurs in a moderate or light way.
Test targets: cucumber gray mold pathogen (Botrytis cinerea).
And (3) test crops: cucumber (jin Chun IV).
Reagent to be tested: 30% of fenpyrazamine-chlorphenesin amide suspending agent (1:3) (7.5% + 22.5%), 3% of fenpyrazamine-chlorphenesin amide microemulsion (1:1) (1.5% + 1.5%), 20% of fenpyrazamine-chlorphenesin amide suspending agent (3:1) (15% + 5%), 24% of fenpyrazamine-chlorphenesin amide water dispersible granules (1:5) (4% + 20%), 20% of fenpyrazamine-chlorphenesin amide emulsifiable concentrate (3:2) (12% + 8%), 30% of fenpyrazamine-chlorphenesin amide wettable powder (5:1) (25% + 5%), 30% of chlorphenesin suspending agent, 30% of fenpyrazamine suspending agent and 50% of boscalid water dispersible granules.
And (3) experimental design: the experiment designed 10 treatments, each of which was repeated 4 times with clear water as a blank. Cell area 20m2Each cell is arranged in random block groups, and adjacent cells are provided with protection rows.
The application time is as follows: the administration was 2 times, with the first administration on day 5/month 14 of 2020, the second administration on day 5/month 21 of 2020, and no other agent administered during the test period.
The investigation method comprises the following steps: and (3) adopting a random five-point sampling method in each cell, investigating 2 plants at each point, investigating all leaves and all fruits of each cucumber, investigating the disease occurrence base number before application, investigating the disease occurrence condition 7 days after the first application and 10 days after the last application, and calculating the disease index and the prevention and treatment effect.
The recording was carried out in stages according to the following classification method:
the fruit damage grading method comprises the following steps:
level 0: no disease spots;
level 1: residual flowers are attacked;
and 3, level: attack of fruit umbilicus;
and 5, stage: the length of the disease spot is less than 10% of the length of the fruit;
and 7, stage: the length of the disease spots accounts for less than 11-25% of the length of the fruits;
and 9, stage: the length of the disease spot accounts for more than 26 percent of the length of the fruit.
Grading damage of leaves:
level 0: no disease spots;
level 1:3 scabs exist on a single leaf;
and 3, level: 4-6 scabs exist on a single leaf;
and 5, stage: 7-10 scabs exist on a single leaf;
and 7, stage: 11-20 scabs exist on a single leaf;
and 9, stage: the single leaf has more than one fourth of the area of the dense diseased spots.
The efficacy is calculated according to the following formula:
the test results are shown in Table 3.
TABLE 3 field test results of the efficacy of Aminopyrazone and Chlorobenzene Ether amide in combination for cucumber Gray mold
As shown in the field efficacy test of Table 3, the blending of fenpyrazamine and chlorphenesin shows good control effect on cucumber gray mold. Compared with a single agent, the control effect of each mixed treatment is improved to different degrees under the condition of reducing the dosage, wherein the control effect is best when the weight ratio of the fenpyrazamine to the chlorphenesin is 1: 3.
Example 4 Aminopyrafen and Chlorobenzene Ether amide combination rape sclerotinia rot field test
The test basis is as follows: the test refers to GB/T17980.35-2000 'test criterion for pesticide effect in field for preventing and treating vegetable rape sclerotinia rot'.
Test site: in the rape base of pier headends of Haian city of Jiangsu province, the soil layer of the test field is deep, the loam is fertile, the terrain is smooth, the organic matter content is 1.9%, the pH value is 6.8, and the irrigation field is good. The field management is good, the cultivation conditions of all test districts are consistent, and the method accords with local test production practices.
Test targets: sclerotinia sclerotiorum (sclerotitiorum) is used.
And (3) test crops: rape (oil grind No. 10).
Test agents: 30% of fenpyrazamine-chlorphenesin amide suspending agent (1:3) (7.5% + 22.5%), 20% of fenpyrazamine-chlorphenesin amide suspending agent (3:1) (15% + 5%), 3% of fenpyrazamine-chlorphenesin microemulsion (1:1) (1.5% + 1.5%), 30% of chlorphenesin suspending agent, 30% of fenpyrazamine suspending agent and 40% of dimethomorph wettable powder.
And (3) experimental design: the experiment designed 7 treatments, each of which was repeated 4 times with clear water as a blank. Cell area 20m2Each cell randomly adopts block arrangement, and adjacent cells are provided with protection rows.
The application time and the application frequency are as follows: the pesticide is applied for 2 times, and is applied for the first time in 12 days 4 months in 2020, at the moment, the rape is in the flowering phase, a small amount of scabs exist in the stem, and a large area of scabs do not appear; the second administration is given in 21 days 4 and 4 in 2020. No other agents were administered during the trial.
The investigation method comprises the following steps: each cell adopts a checkerboard type 10-point survey, 5 plants (50 plants/cell) are surveyed for 2 times, the disease base number is surveyed before medicine application, the disease plant number of each level of each cell is surveyed 10 days after the last medicine application, and the disease index and the prevention and treatment effect are calculated.
The rating recording was performed according to the following rating method:
level 0: no disease spots;
level 1: the disease is slightly developed, and the area of the disease spot accounts for less than 5% of the whole main stem area;
and 3, level: the disease is slightly attacked, and the area of the disease spots accounts for 6 to 15 percent of the whole main stem area;
and 5, stage: the disease is moderate, and the area of the disease spot accounts for 16 to 25 percent of the whole main stem area;
and 7, stage: the disease is highly developed, and the area of the disease spot accounts for 26 to 50 percent of the whole main stem area;
and 9, stage: the disease is seriously ill, and the area of the disease spot accounts for more than 50 percent of the whole main stem area or withers and dies.
The efficacy is calculated according to the following formula:
the test results are shown in Table 4.
TABLE 4 Aminopyrafen and Chlorobenzene Ether amide combination rape sclerotinia rot field drug effect test results
As can be seen from the field efficacy test in Table 4, the mixture of fenpyrazamine and chlorphenesin amide shows good control effect on sclerotinia rot of colza. 10 days after the second application, the control effects of 30% of the fenpyrazamine-chlorophenoxylate amide suspending agent (1:3) (7.5% + 22.5%), 20% of the fenpyrazamine-chlorophenoxylate amide suspending agent (3:1) (15% + 5%) and 3% of the fenpyrazamine-chlorophenoxylate amide microemulsion (1:1) (1.5% + 1.5%) on sclerotinia rot of colza are 81.67%, 82.47% and 83.05%, respectively.
In conclusion, through indoor toxicity measurement and field efficacy tests, the bactericidal composition containing the fenpyrazamine and the chlorophenoxyacetic acid amide has a good control effect on fungal diseases of vegetables and fruits, is safe to target crops, has an obvious control effect, is superior to a single agent in the aspects of delaying the generation of drug resistance and prolonging the persistence, and can effectively reduce the cost and reduce the drug residue.
Claims (10)
1. The bactericidal composition containing fenpyrazamine and chlorobenzene ether amide is characterized by comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is fenpyrazamine, the active ingredient B is chlorobenzene ether amide, and the mass ratio of the fenpyrazamine to the chlorobenzene ether amide is 1: 50-50: 1.
2. The bactericidal composition according to claim 1, wherein the mass ratio of fenpyrazamine to chlorphenesin is 1: 30-10: 1.
3. The bactericidal composition according to claim 1, wherein the mass ratio of fenpyrazamine to chlorphenesin is 1: 5-5: 1.
4. The bactericidal composition according to claim 1, wherein the active ingredient is present in the bactericidal composition in an amount of 1 to 80 wt%, preferably 5 to 60 wt%, based on 100 wt% of the total weight of the bactericidal composition.
5. The bactericidal composition of claim 1, wherein the bactericidal composition is formulated into any agriculturally acceptable formulation.
6. The bactericidal composition of claim 5, wherein the formulation is selected from any one of powders, granules, soluble powders, soluble granules, soluble tablets, water dispersible granules, wettable powders, water dispersible tablets, microcapsule suspensions, dispersible solutions, emulsifiable concentrates, aqueous emulsions, microemulsions, suspending agents, suspoemulsions, and soluble solutions.
7. The bactericidal composition according to claim 6, wherein the formulation is any one of a suspending agent, a microemulsion, an emulsifiable concentrate, a water dispersible granule and a wettable powder.
8. Use of the fungicidal composition according to any one of claims 1 to 7 for controlling fungal diseases of agricultural plants.
9. The use of claim 8, wherein the fungal diseases are one or more of gray mold, sclerotinia sclerotiorum, sheath blight and late blight;
preferably, the fungal diseases are gray mold and sclerotinia sclerotiorum.
10. Use according to claim 8, wherein the plant is a protected or field crop.
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| CN104557709A (en) * | 2013-10-23 | 2015-04-29 | 华中师范大学 | Pyrazole amide compound containing diphenyl ether, and application and pesticide composition of pyrazole amide compound |
| CN108200909A (en) * | 2016-12-20 | 2018-06-26 | 燕化永乐（乐亭）生物科技有限公司 | A kind of bactericidal composition |
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| CN108770856A (en) * | 2018-08-17 | 2018-11-09 | 陕西上格之路生物科学有限公司 | A kind of bactericidal composition containing fluorine azoles bacterium acyl azanol and amine benzene pyrrole bacterium ketone |
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| CN104557709A (en) * | 2013-10-23 | 2015-04-29 | 华中师范大学 | Pyrazole amide compound containing diphenyl ether, and application and pesticide composition of pyrazole amide compound |
| CN108200909A (en) * | 2016-12-20 | 2018-06-26 | 燕化永乐（乐亭）生物科技有限公司 | A kind of bactericidal composition |
| CN108605961A (en) * | 2018-05-23 | 2018-10-02 | 深圳诺普信农化股份有限公司 | A kind of bactericidal composition containing fluorine azoles bacterium acyl azanol and its application |
| CN108770856A (en) * | 2018-08-17 | 2018-11-09 | 陕西上格之路生物科学有限公司 | A kind of bactericidal composition containing fluorine azoles bacterium acyl azanol and amine benzene pyrrole bacterium ketone |
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