CN114145880A - Multichannel modular animal inhales exposes case and exposes system - Google Patents
Multichannel modular animal inhales exposes case and exposes system Download PDFInfo
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- CN114145880A CN114145880A CN202111522101.1A CN202111522101A CN114145880A CN 114145880 A CN114145880 A CN 114145880A CN 202111522101 A CN202111522101 A CN 202111522101A CN 114145880 A CN114145880 A CN 114145880A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
- A61D7/04—Devices for anaesthetising animals by gases or vapours; Inhaling devices
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- Life Sciences & Earth Sciences (AREA)
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Abstract
The invention discloses a multi-channel modular animal suction exposure box and an exposure system, which comprise a test box, wherein a side door is hinged on a side opening of the test box, a fog inlet, a detection port, an air inlet and an air outlet are arranged on the test box, and experimental animals are placed in the test box. The multi-channel modularized animal inhalation exposure box is suitable for contamination experiment research with controllable dose and condition in a laboratory and outdoor exposure research in actual environment, namely, an experimental animal can be exposed to atomized aerosol particles, simulated contamination gas, tail gas, smoke and the like and can be exposed to the external actual atmospheric environment; and the simultaneous exposure research of a plurality of groups of animals, different target pollutants and different toxicant exposure doses can be realized simultaneously through modularized combination, the exposure system can be ingested through the natural respiration of the animals, the purpose of actual environment exposure or specific environment exposure is achieved, and the convenience and diversity of the research are realized.
Description
Technical Field
The invention relates to the technical field of exposure experiment devices, in particular to a multi-channel modular animal suction exposure box and an exposure system.
Background
As the health standard of the world health organization on air pollution is gradually tightened, the research on the health hazard of the air pollution is particularly important. The research result of the global disease burden in 2019 shows that the air pollution is high in the fourth position of global death cause, and the air pollutants can directly or carry a large amount of organic chemical components to enter the bronchus, alveolus or even blood through breathing, so that the respiratory system, the cardiovascular system, the immune system, the nervous system and the like of human beings are affected. Therefore, health impact studies of air pollution exposure are of great concern. However, the existing scientific research results can not form comprehensive knowledge of the air pollution health effect and the mechanism thereof, and can not meet the requirement of making environmental health decisions by governments of various countries. The research experiment of exposing animals to air pollution can fully demonstrate the pathophysiological mechanism of air pollution, so the research of the experiment of exposing animals to air pollution is an effective means.
At present, in domestic and foreign researches, animal exposure experimental researches are carried out by adopting a mode of nasal cavity instillation or tracheal instillation, but the actual state of the animal exposure experimental researches is different from that of air pollutants inhaled into a respiratory tract. In part of researches, experimental animals are fixed and subjected to mouth and nose exposure, the exposure time of the method is limited, the autonomous activity of the animals is limited in the exposure process, and the interference on the physiological indexes of the animals cannot be avoided. In addition, the damage of most air pollutants to the organism is long-term and chronic, and the damage caused by one-time large-dose exposure is not in line with the chronic pathological process caused by the repeated inhalation of organisms for a long time.
The exposure box can simulate the exposure condition of animals to air pollutants in an actual environment, but most of the existing whole body exposure systems are commercial integrated products, a computer control system, an air treatment system and an exposure functional unit are arranged in a large cabinet body, and although exposure parameters are automatically adjusted, the whole product is expensive in price, too large in size, occupies a large space in a laboratory and is inconvenient to move; the exposure box and the exposure system for the animal adsorption contamination experiment can only passively inhale contamination through animals, and cannot actively inhale exposure in actual environment. To this end, a multi-channel animal suction exposure cabinet and exposure system is needed to address the above problems.
Disclosure of Invention
The invention aims to provide a multi-channel modular animal inhalation exposure box, which solves the problems in the prior art and enables simultaneous exposure research on multiple groups of animals, different target pollutants and different toxicant exposure doses to be realized under actual environment exposure or specific environment exposure.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a multi-channel modular animal inhalation exposure box which comprises a test box, wherein a side door is hinged to a side opening of the test box, a fog inlet, a detection port, an air inlet and an air outlet are formed in the test box, an experimental animal is placed in the test box, the side door can be opened to be communicated with the outside atmosphere, the fog inlet is used for being communicated with a contaminated air source after different reagents are atomized, and the air inlet is used for being communicated with a purified air source or a contaminated air source.
Preferably, two opposite side faces of the test box are respectively hinged with one side door, and the two side doors are respectively provided with an observation window and a handle.
Preferably, one be provided with air inlet, another on the side door be provided with the gas outlet on the side door, advance the fog mouth with detect the mouth set up respectively in on two other sides of proof box, advance the fog mouth detect the mouth the air inlet with all be provided with the screw thread on the gas outlet.
Preferably, a circle of convex partition plates are respectively arranged on two opposite side openings of the test box, and filter membranes are detachably connected to the partition plates.
Preferably, a fan is detachably arranged on the top surface of the test box.
Preferably, a hygrothermograph is arranged on the side wall of the test chamber.
Preferably, the material of the test box is medical stainless steel 316L, and the length of the test box is 500mm, the width of the test box is 400mm, and the height of the test box is 200 mm.
The invention also relates to a multi-channel modular animal inhalation exposure system which comprises the multi-channel modular animal inhalation exposure box, an atomized contaminated air source assembly and a purified air source assembly or contaminated air source assembly, wherein an air inlet of the multi-channel modular animal inhalation exposure box is communicated with the purified air source assembly or contaminated air source assembly, a mist inlet is communicated with the atomized contaminated air source assembly, and an air outlet is communicated with the outside atmosphere.
Preferably, the atomized toxicant exposure air source assembly comprises a drying filter tube, a medical atomizer, a rotor flow meter and a clean air bottle which are sequentially communicated with the mist inlet; the purified air source component comprises a rotor flow meter, an air filter and an air pump which are sequentially communicated with the air inlet; the gas source component comprises a diluter and a gas source which are communicated with the gas inlet in sequence.
Preferably, the multichannel modular animal inhalation exposure box is provided with a plurality of multichannel modular animal inhalation exposure boxes, and an air inlet of each multichannel modular animal inhalation exposure box is respectively communicated with the purification air source component or the contamination air source component, and a mist inlet is respectively communicated with the atomization contamination air source component.
Compared with the prior art, the invention has the following technical effects:
the multi-channel modularized animal inhalation exposure box is suitable for contamination experiment research with controllable dose and condition in a laboratory and outdoor exposure research in actual environment, namely, an experimental animal can be exposed to atomized aerosol particles, simulated contamination gas, tail gas, smoke and the like and can be exposed to the external actual atmospheric environment; and the simultaneous exposure research of a plurality of groups of animals, different target pollutants and different toxicant exposure doses can be realized simultaneously through modularized combination, the exposure system can be ingested through the natural respiration of the animals, the purpose of actual environment exposure or specific environment exposure is achieved, and the convenience and diversity of the research are realized.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic diagram of the construction of a multi-channel modular animal inhalation exposure system of the present invention;
FIG. 2 is a first schematic view of the multi-channel modular animal intake exposure box of the present invention;
FIG. 3 is a schematic view of the configuration of the multi-channel modular animal intake exposure box of the present invention;
FIG. 4 is a schematic view of the configuration of the multi-channel modular animal intake exposure box of the present invention;
wherein: 1-test box, 2-fog inlet, 3-detection port, 4-partition board, 5-side door, 6-observation window, 7-handle, 8-air inlet, 9-hygrothermograph, 10-air outlet, 11-fan, 12-air pump, 13-medical atomizer, 14-dry filter tube, 15-rotameter, 16-clean air bottle, 17-air filter.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive effort based on the embodiments of the present invention, are within the scope of the present invention.
The invention aims to provide a multi-channel modular animal suction exposure box and an exposure system, which are used for solving the problems in the prior art and realizing the simultaneous exposure research on a plurality of groups of animals, different target pollutants and different toxicant exposure doses under actual environment exposure or specific environment exposure.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
Example 1
As shown in fig. 1 to 4: the embodiment provides a multichannel modularization animal inhales and exposes case, including proof box 1, it has side door 5 to articulate on the side opening of proof box 1, is provided with into fog mouth 2 on the proof box 1, detects mouth 3, air inlet 8 and gas outlet 10, is used for placing the experimental animal in the proof box 1, side door 5 can open with external atmosphere intercommunication, advances the gas source that infects after fog mouth 2 is used for the atomizing of different reagents, air inlet 8 is used for communicateing air purification source or the gas source that infects. If the experiment is carried out in a short period, the experimental animal can be directly placed in the test box 1, and if water needs to be fed, the ventilation cage for feeding can be placed for storing the experimental animal.
In this embodiment, a "multi-channel" inhalation mode of contaminated gas can be realized, that is: the side door 5 can be used for exposing the external actual atmospheric environment after being opened, and the air purified by the filter membrane can enter the test box 1 after the filter membrane is embedded into the partition plate 4; the inlet 2 can be used for exposing atomized aerosol particles; the air inlet 8 can be used for simulating exposure of contaminated gas, tail gas, smoke and the like, or introducing clean air to ensure normal breathing of experimental animals.
Two relative sides of proof box 1 are articulated respectively (through the hinge) have a side door 5 (be provided with the sealing strip on the side door 5 and seal), all are provided with observation window 6 and handle 7 on two side doors 5. An air inlet 8 is formed in one side door 5, an air outlet 10 is formed in the other side door 5, the fog inlet 2 and the detection port 3 are respectively formed in the other two side faces of the test box 1, and threads are formed in the fog inlet 2, the detection port 3, the air inlet 8 and the air outlet 10. After the aerosol concentration in the exposure box is stable, the detection port 3 is connected with an external aerosol detector, the concentration of particles in the air entering the test box 1 can be detected, and the height of the detection port 3 is matched with the height of the activity breath of an animal in the test box 1. The detection port 3 is connected with a catheter through an L-shaped adapter, the catheter can be rotated to a point position to be detected, and after the first point is detected, the position of the catheter is changed to detect a second point position; the tee joint is connected with the guide pipe, and the average concentration of air at three points can be measured, so that the toxicant is ensured to be uniformly dispersed in the test box 1 in an aerosol state. Be provided with convex baffle 4 of round on two relative side openings of proof box 1 respectively, can dismantle on the baffle 4 and be connected with the filter membrane, the filter membrane is for reaching the HEPA standard's filter membrane, and efficiency reaches 99% to the granule removal of micron, proof box 1 behind the embedding filter membrane can regard as the control group. The depth of the partition plate 4 on the test box 1 is matched with that of the side doors 5, when the two side doors 5 are opened, a filter membrane can be embedded into the partition plate 4, so that ambient air enters the test box 1 after being purified by the filter membrane, and meanwhile, the fog inlet 2 and the detection port 3 on the front side and the rear side of the test box 1 are closed, and the air entering the test box 1 is ensured to be purified by the filter membrane; when the two side doors 5 are opened and the partition plate 4 is not provided with a filter membrane, the test chamber 1 can be directly exposed to the actual ambient air.
A fan 11 is detachably disposed on the top surface of the test chamber 1, so that the atomized toxicant is uniformly dispersed inside the test chamber 1. The side wall of the test box 1 is provided with a hygrothermograph 9 which can observe the temperature and humidity change in the test box 1 in real time; the hygrothermograph 9 is fixed on the inner side of the test box 1, the numerical value can be seen through the observation window 6 on the box door, and the observation window 6 is made of transparent organic glass. The material of the test box 1 is medical stainless steel 316L, the length of the test box 1 is 500mm, the width is 400mm, and the height is 200mm, so that a light-proof environment can be provided for experimental animals, the human interference can be reduced, and the material is non-toxic to the animals; can accommodate a maximum of two separate IVC cages, each of which can accommodate 5-6 adult mice. If the two test boxes 1 are used in a combined mode, two groups of animals can be exposed to the actual environment and the filtered air environment respectively, researchers can directly compare differences among the animals in different exposure environments, and influences of the exposure environments on the living states and physiological indexes of the animals can be reflected visually.
The embodiment also relates to a multi-channel modularized animal inhalation exposure system, which comprises the multi-channel modularized animal inhalation exposure box, an atomized contaminated air source component and a purified air source component or contaminated air source component, wherein an air inlet 8 of the multi-channel modularized animal inhalation exposure box is communicated with the purified air source component or contaminated air source component, an air inlet 2 is communicated with the atomized contaminated air source component, and an air outlet is communicated with the outside atmosphere. In the embodiment, the air inlet 8 can be communicated with the air pump 12 through a rotor flow meter 15 and an air filter 17 or communicated with a contaminated air source (a simulated contaminated air source such as contaminated air, automobile exhaust, flue gas and the like) through a diluter, the mist inlet 2 is communicated with a clean air bottle 16 through a drying filter pipe 14 and a medical atomizer 13 in sequence, the rotor flow meter 15 is communicated with the clean air bottle, and the air outlet 10 is communicated with the outside atmosphere. Each part can be used as an independent module, the exposure box is correspondingly connected with different air inlet assemblies (a purification air source assembly or a contamination air source assembly) and an atomization contamination air source assembly, and the exposure experiment of different pollution sources or different experimental animals can be simulated according to the experiment requirements of different variables.
The atomization gas source component comprises a drying filter pipe 14, a medical atomizer 13, a rotor flow meter 15 and a clean air bottle 16 which are sequentially communicated with the mist inlet 2, and can simulate the exposure experiment of different atomization gas sources for the experimental animals according to the experiment requirement. The purified air source component comprises a rotor flow meter 15, an air filter 17 and an air pump 12 which are sequentially communicated with the air inlet 8, and provides a normal breathing environment for the experimental animal according to the experimental requirement. The contamination air source component comprises a diluter and a contamination air source which are sequentially communicated with the air inlet 8, the air pump can be increased according to different contamination air sources, and the exposure experiment of different contamination air sources on experimental animals can be simulated according to the experiment requirements. The test boxes 1 can share one clean air bottle 16, the rotameter 15 can adjust the flow of carrier gas entering the medical atomizer 13 and the test boxes 1 to ensure the stability of atomization, and the toxicant in the liquid medicine cup of the medical atomizer 13 enters the test boxes 1 through the fog inlet 2 after being atomized.
The multi-channel modularized animal inhalation exposure box is provided with a plurality of multi-channel modularized animal inhalation exposure boxes, an air inlet 8 of each multi-channel modularized animal inhalation exposure box is respectively communicated with a purified air source component or a contaminated air source component, and a fog inlet 2 is respectively communicated with an atomized contaminated air source component.
Example 2
This example is an outdoor actual environment exposure experiment: two test chambers 1 were placed in the open air, two separate aeration cages were placed in each chamber, five mice were placed in each cage, and the mice were free to access water and food. Exposure group: the two side doors 5 of the test chamber 1 are opened without any other treatment, and the state of the mouse can be observed at any time. Control group: the test chamber 1 is opened on both sides 5 and a filter membrane of suitable dimensions is inserted into the partition 4 (this is done)Department adopts the HEPA filter membrane), the air passes through the filter membrane and gets into in the experimental box 1, will advance fog mouth 2 simultaneously and detect 3 pipe caps for the mouth stifled, guarantees to get into the filtration of experimental box 1's outside air through the HEPA filter membrane. PM in actual atmospheric environment on the same day is detected by adopting aerosol detector2.5Concentration value, after the air in the test chamber 1 is fully purified, the PM of the control group is measured2.5The concentration value is recorded. After 8 hours of exposure, the mice of the exposure group and the control group are normal in state and have no adverse reaction, which shows that the actual effect of the test box 1 can meet the outdoor experiment requirement and can ensure the feasibility of the control group of the outfield experiment.
Example 3
This example is a laboratory fogging exposure experiment: two groups of experimental animals are adopted, and the first group is a normal saline control; the second group is the use of PM2.5Loading polycyclic aromatic hydrocarbon pollutants on the model particles, resuspending the loaded particles by physiological saline, and storing the particles in a refrigerator at the temperature of 20 ℃ below zero for later use.
In the exposure experiment, the test box 11 is used in cooperation with the air pump 12, the medical atomizer 13, the clean air bottle 16, the fan 11, the high-efficiency air filter 17 and the hygrothermograph 9 to respectively expose two groups of experimental animals. The two test chambers 1 are connected in parallel, a bottle of clean air 16 is used for providing carrier gas required by atomization, the flow rate of the carrier gas of the corresponding test chamber 1 is controlled through a rotor flow meter 15, each test chamber 1 is provided with a toxicant through a medical atomizer 13, and tail gas generated in the experimental process is discharged through an air outlet 10. In the first set of test chambers 1, the air pump 12 was turned on and clean air passed through the air filter 17 was admitted to the test chamber 1 and the air flow through the air inlet 8 was regulated using the rotameter 15. In the second group of test boxes 1, the clean air bottle 16 is unscrewed, so that the clean air carries atomized pollutants in the medical atomizer 13 to contaminate experimental animals, and the flow of the carrier gas is adjusted by the rotameter 15.
In addition, in each embodiment, a plurality of test boxes 1 can be connected in parallel in a modularized mode to perform a plurality of groups of exposure experiments simultaneously, each test box 1 shares one air pump 12, the flow rate of carrier air of the corresponding exposure box is controlled through one rotor flow meter 15, each exposure box is provided with a poisoning solution through one medical atomizer 13 or is actively exposed through a direct exposure channel at the same time, target pollutants can be freely selected, and the concentration of particulate matters in an exposure area can be adjusted through adjusting the flow rate of air in an air inlet 8 and the flow rate of atomized carrier air; the actual exposure scene can be simulated, the animal can be exposed by active natural respiration contamination, and the animal can move freely without influencing the normal growth of the animal; compared with the existing large-scale whole body exposure device, the device has smaller volume, is convenient for carrying out outdoor experiments in actual environment, has lower cost and simple structure, and is convenient to clean; exposure of groups of animals, different target contaminants or different exposure doses can be simultaneously achieved through modular assembly. The embodiment is suitable for whole-body dynamic exposure of common small animals, can atomize the toxic agents and can realize harmful gas exposure; through exposing the free equipment of case and exposure equipment, experimental animals can carry out that the pollutant is adjustable, and concentration is measurable, and the inhalation exposure research that the exposure of toxicant dose is stable and time is controllable for animal inhalation exposure is laminated actual environmental condition more and is exposed, and the research result has more the referential.
The principle and the implementation mode of the present invention are explained by applying specific examples in the present specification, and the above descriptions of the examples are only used to help understanding the method and the core idea of the present invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.
Claims (10)
1. A multi-channel modular animal intake exposure cabinet characterized by: the device comprises a test box, the side opening of the test box is hinged with a side door, the test box is provided with a fog inlet, a detection port, an air inlet and an air outlet, experimental animals are placed in the test box, the side door can be opened to be communicated with the outside atmosphere, the fog inlet is used for communicating toxic gas sources after different reagents are atomized, and the air inlet is used for communicating a purified gas source or a toxic gas source.
2. The multi-channel modular animal intake exposure cabinet of claim 1, wherein: the side door is hinged to two opposite side faces of the test box respectively, and the observation window and the handle are arranged on the two side doors.
3. The multi-channel modular animal intake exposure cabinet of claim 2, wherein: one be provided with air inlet, another on the side door be provided with the gas outlet on the side door, advance the fog mouth with detect the mouth set up respectively in on two other sides of proof box, advance the fog mouth detect the mouth the air inlet with all be provided with the screw thread on the gas outlet.
4. The multi-channel modular animal intake exposure cabinet of claim 2, wherein: and two opposite side openings of the test box are respectively provided with a circle of convex partition plates, and the partition plates are detachably connected with filter membranes.
5. The multi-channel modular animal intake exposure cabinet of claim 1, wherein: the top surface of the test box is detachably provided with a fan.
6. The multi-channel modular animal intake exposure cabinet of claim 1, wherein: and a hygrothermograph is arranged on the side wall of the test box.
7. The multi-channel modular animal intake exposure cabinet of claim 1, wherein: the material of the test box is medical stainless steel 316L, and the length of the test box is 500mm, the width is 400mm, and the height is 200 mm.
8. A multi-channel modular animal inhalation exposure system characterized by: comprising the multi-channel modular animal inhalation exposure box of any one of claims 1-7, an atomized contaminated air source assembly, and a decontamination air source assembly or contaminated air source assembly, wherein the air inlet of the multi-channel modular animal inhalation exposure box is communicated with the decontamination air source assembly or contaminated air source assembly, the mist inlet is communicated with the atomized contaminated air source assembly, and the air outlet is communicated with the outside atmosphere.
9. The multi-channel modular animal intake exposure system of claim 8, wherein: the atomization contamination gas source assembly comprises a drying filter pipe, a medical atomizer, a rotor flow meter and a clean air bottle which are sequentially communicated with the mist inlet; the purified air source component comprises a rotor flow meter, an air filter and an air pump which are sequentially communicated with the air inlet; the gas source component comprises a diluter and a gas source which are communicated with the gas inlet in sequence.
10. The multi-channel modular animal intake exposure system of claim 8, wherein: the multi-channel modular animal inhalation exposure box is provided with a plurality of air inlets, each air inlet is communicated with the purification air source component or the contamination air source component, and the fog inlets are communicated with the atomization contamination air source component.
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| CN202111522101.1A CN114145880A (en) | 2021-12-13 | 2021-12-13 | Multichannel modular animal inhales exposes case and exposes system |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114788498A (en) * | 2022-04-07 | 2022-07-26 | 广州医科大学附属第一医院（广州呼吸中心） | Tail gas generating device for pollutant gas exposure cabin experiment |
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| CN117356453A (en) * | 2023-10-09 | 2024-01-09 | 安徽理工大学第一附属医院 | Animal respiratory dust-dyeing test device and test method thereof |
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