CN106769811A - A kind of air microbe tests consersion unit and application - Google Patents
A kind of air microbe tests consersion unit and application Download PDFInfo
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- CN106769811A CN106769811A CN201710028214.3A CN201710028214A CN106769811A CN 106769811 A CN106769811 A CN 106769811A CN 201710028214 A CN201710028214 A CN 201710028214A CN 106769811 A CN106769811 A CN 106769811A
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/06—Investigating concentration of particle suspensions
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
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- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D27/00—Simultaneous control of variables covered by two or more of main groups G05D1/00 - G05D25/00
- G05D27/02—Simultaneous control of variables covered by two or more of main groups G05D1/00 - G05D25/00 characterised by the use of electric means
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/06—Investigating concentration of particle suspensions
- G01N15/075—Investigating concentration of particle suspensions by optical means
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1486—Counting the particles
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Abstract
The invention discloses a kind of air microbe experiment consersion unit and application.The equipment is used for the aerial reactions change of microorganisms, including reaction cabin, empty gasification generator and air sampler, it is provided with air inlet on the side wall of reaction cabin to be connected with empty gasification generator, relative opposite side wall is provided with gas outlet and is connected with air sampler；Compartment roof is provided with fluorescent lamp and uviol lamp, and fan and Temperature Humidity Sensor are provided with cabin；The volume of the reaction cabin is 150 500 liters, and inwall scribbles hydrophobic coating.The hydrophobic surface of reaction cabin, fan disturbance, can largely ensure that microorganism is in air；Its volume can meet different open-assembly times with subsequent acquisition detection needs；The various devices of nacelle over-assemble can change the different condition of the air reaction of microorganism, and can real-time control, real-time detection, help to further appreciate that the microorganism especially aerial propagation characteristic of pathogenic bacteria.
Description
Technical field
The invention belongs to aerosol art, and in particular to a kind of microorganisms the method for reactions change and set in atmosphere
It is standby, the method and apparatus of the exposure reaction of the aerial activity of microorganisms, appeal and change in physical properties.
Background technology
Air is the important medium of microorganisms spreading, many human disease bacterium air-borne transmissions, can produce communicable diseases and break out
It is popular.Microorganism in air is including bacterium, fungi, virus, Richettsia, mycoplasma and Chlamydia etc..These microorganisms
There are many in grain causes the pathogen of infection in respiratory system, such as haemophilus influenzae, legionella pneumophilia, influenza virus etc..
The outburst that produces communicable diseases, such as tuberculosis, measles, Q heat, pig stream are easy in some special occupation environment, crowd massing place etc.
Sense, anthrax, legionella pneumophilia disease etc..Research shows that air borne is the important channel that pathogen is propagated.
Microorganism in atmosphere can by aerosol transmission, due to the diversity of species, its survival ability in the environment,
Virulence change performance is different, therefore the change of microbial aerosol is also in variation.The particulate matter of different-grain diameter is in air
In propagation characteristic and distance it is different.For example, the propagation distance of small size particle is farther out, the propagation distance of large-size particles thing
It is nearer.Additionally, microorganism is propagated in atmosphere influenceed by various environmental factors, including temperature, humidity, electromagnetic radiation and air
Chemical group is graded.Temperature is the key factor for influenceing microbial survival.The influence radiated to microbial activity is relevant with wavelength, ripple
The strong radiation of length, energy, such as ultraviolet radioactive can destroy the DNA of pathogen；Long wave and visible ray can influence cell center line
The generation of cytochromes in plastochondria.Outside atmosphere chemical compositions are complex, and the mechanism of the influence to microorganism is also more
It is complicated.
Therefore, it is necessary under controllable environmental factor, to microorganism, change includes its activity for a long time in atmosphere
Studied.This air borne to evaluating microorganism, it is very to study its aerial physical change and biological decay etc.
It is necessary.
Existing air microbe research device is most of it cannot be guaranteed that microorganism stops in atmosphere for a long time, Er Qiehuan
Border factor poor controllability, open-assembly time is short, it is impossible to enough that the change that pathogenic bacteria occur in atmosphere is apparent from.Microorganism
There occurs that what it is not immediately clear actually in atmosphere, the aerial pathogenic change of such as microorganism and propagation understand
Not enough.The microbial exposure device reported in document is in addition, there will be, general volume is larger, high cost, and is difficult to thoroughly sterilizing,
Easily cause the cross pollution of many experiments reaction.Also study to be transferred to air microbe in liquid phase and reacted, design liquid phase
Exposure reaction, it is impossible to react its aerial truth.
The content of the invention
The main object of the present invention is a kind of method and apparatus of microorganisms of design reactions change in atmosphere, is micro-
The air borne of bioaerosol provides an ideal experiment place, and can real-time control items response parameter it is (warm and humid
Degree, illumination, open-assembly time, to ensure that it is in air medium), the oxidative damage and drying effect of microorganism are detected, study its hair
Physical change and biological decay in raw, propagation etc..
Technical scheme is as follows：
A kind of air microbe tests consersion unit, including reaction cabin, empty gasification generator and air sampler, in reaction
The side wall in cabin is provided with air inlet and is connected with empty gasification generator, and relative opposite side wall is provided with gas outlet and air sampling
Device is connected；Compartment roof is provided with fluorescent lamp and uviol lamp, fan and Temperature Humidity Sensor is provided with cabin, and set on bulkhead
There is back up ventilation mouthful, wherein：The volume of the reaction cabin is 150-500 liters, and cabin inner walls scribble hydrophobic coating.
The nacelle of the reaction cabin is preferably cuboid, is made using stainless steel plate, and cabin inner walls scribble one layer of hydrophobic painting
Layer, to reduce absorption and deposition of the microbe granular in inwall.Coating material is preferably silicon nano-resin, and it also has high temperature resistant
With the characteristic of ultraviolet.In one embodiment of the invention, the size of reaction nacelle is 700mm × 500mm × 500mm (length
× wide × high), volume is 175 liters, can meet requirement of the bioaerosol sampler to volume of air.For example, using
During BioSampler samplers, sampling flow is 12.5L/min, can gather 10min；Sampled using BioStage and Andersen
During device, sampling flow is 28.3L/min, can gather 5min.
Empty gasification generator is connected with the air inlet of reaction cabin, and aerosolized microorganism is passed through into nacelle.Further,
Drying tube can be added between empty gasification generator and air inlet, moisture in microbe granular is reduced, reduces particle diameter, mitigated
Quality, reduces its free settling.The empty gasification generator can use respiration bottle, and carrier gas is done using nitrogen, will be aerosolized
Microbe granular is passed through reaction cabin.
The gas outlet of reaction cabin is connected with air sampler, and the air sampler is biological aerosol sampler, collection
Reacted microorganism, gained sample can be used to detect the indexs such as activity, oxidative damage and drying effect.Meanwhile, the air
Experiment on Microbiology consersion unit can also further include concentration detection apparatus, and such as optical particle counter (OPC) or ultraviolet-gas is molten
Glue particle size analyzer (UV-APS) etc., the gas outlet of reaction cabin is connected with concentration detection apparatus, can micro- life in real-time detection cabin
The concentration of composition granule.
Installed in the fluorescent lamp and uviol lamp of compartment roof, illumination and ultraviolet radioactive are respectively configured to provide.Uviol lamp may be used also
For being cabin sterilization.
The fan can use simple ceiling fan or scavenger fan, for disturbing the air-flow in cabin, reduce microorganism
The sedimentation of grain, increases its aerial stop and reaction time.
The Temperature Humidity Sensor preferably uses split type sensor, and its probe is passed through cabin interior, for monitoring cabin
Interior temperature and humidity, and display pannel is suspended on cabin outside, is easy to real time readouts.
Further, the cabin side wall in reaction cabin is provided with back up ventilation mouthful, can be led to in cabin by back up ventilation mouthful
Enter various reaction gases, such as O3, for carrying out experiment of the gas to air microbe activity influence.When not using, plug can be used
Block.
Further, blower fan or ventilation fan can be set in cabin bottom, increases air agitation.
When in use, first passing through sky gasification generator will be aerosolized for air microbe experiment consersion unit of the invention
Microbe granular is passed through in reaction cabin through air inlet, by reaction cabin for microorganism provides various reaction conditions, for example：Fluorescent lamp
Visible ray illumination is provided, uviol lamp provides ultraviolet radioactive, the gas disturbance situation in fan control cabin, Temperature Humidity Sensor control
Reaction temperature and humidity in cabin processed, back up ventilation mouthful can be passed through the gases such as vapor, ozone.Microorganism after the completion of reaction
Gathered by air sampler by gas outlet, carry out coherent detection.
Beneficial effects of the present invention：The present invention is first for the change that microorganisms occur in atmosphere provides research hand
Section and method, the volume of reaction cabin can meet different open-assembly times with subsequent acquisition detection needs.Secondly, the present invention is by cabin
The various devices of body over-assemble, can change the different condition of the air reaction of microorganism, and can real-time control, real-time detection.Additionally,
There is the exposing device of research and development hydrophobic surface, fan to disturb, and can largely ensure that microorganism is in atmosphere.This grinds
Studying carefully means and method helps to further appreciate that the microorganism especially aerial propagation characteristic of pathogenic bacteria.
Brief description of the drawings
Fig. 1 air microbes test reaction cabin stereogram, wherein：1 is reaction nacelle；2 is air inlet；3-1 is daylight
Lamp；3-2 is uviol lamp；4 is fan；5 is Temperature Humidity Sensor；6 is gas outlet；7 is back up ventilation mouthful.
Specific embodiment
Below in conjunction with the accompanying drawings, the present invention is further illustrated by embodiment.
Using shown in Fig. 1 air microbe experiment reaction cabin microorganisms it is aerial activity, appeal and
Change in physical properties, air microbe experiment reaction cabin includes reaction nacelle 1, and air inlet is provided with the side of reaction nacelle 1
2, relative opposite side is provided with gas outlet 6, and compartment roof sets fluorescent lamp 3-1 and uviol lamp 3-2, and is hung with fan 4, cabin
Temperature Humidity Sensor is inside provided with, back up ventilation mouthful 7 is also left on bulkhead.
The reaction nacelle 1 is cuboid, is made using stainless steel plate, and its size is 700mm × 500mm × 500mm (long
× wide × high), volume is 175 liters, can meet requirement of the bioaerosol sampler to volume of air.For example, using
During BioSampler samplers, sampling flow is 12.5L/min, can gather 10min；Sampled using BioStage and Andersen
During device, sampling flow is 28.3L/min, can gather 5min.
Air inlet 2 is located at the left side in cabin, internal diameter 50mm.Air inlet 2 can be connected with empty gasification generator, will be aerosolized
Microorganism be passed through nacelle.Gas outlet 6 is located at the right side in cabin, internal diameter 50mm.Gas outlet 3 is connected with sampler, collection exists
Reacted microorganism in cabin, gained sample can be used to detect the indexs such as activity, oxidative damage and drying effect；May be used also simultaneously
It is connected with concentration detection apparatus, such as optical particle counter (OPC) or ultraviolet-aerosol particle size analyzer (UV-APS), can
The concentration of microbe granular in real-time detection cabin.
Each one of fluorescent lamp 3-1 and uviol lamp 3-2 is installed in compartment roof, for providing illumination and ultraviolet radioactive.It is ultraviolet
Lamp 3-2 can also be used to be cabin sterilization.
Fan 4 uses simple ceiling fan, flabellum diameter 420mm for disturbing the air-flow in cabin, to reduce microbe granular
Sedimentation, increases its aerial stop and reaction time.
Temperature Humidity Sensor 5 is split type, and probe is passed through cabin interior, for monitoring the humiture in cabin, error model
Enclose ± 2%；Display pannel is suspended on cabin outside, is easy to real time readouts.
In cabin side, wall leaves back up ventilation mouthful 7, its internal diameter 20mm, can be to being passed through various reaction gases, example in cabin
Such as O3, for carrying out experiment of the gas to air microbe activity influence.When not using, can be blocked with plug.
Concrete operations are as follows：
1) respiration bottle (Collison Nebulizer) is connected with air inlet 2, uses nitrogen (N2) carrier gas is done, gas is molten
The microbe granular of gel is passed through reaction cabin；
2) by the switch or condition of each part in control cabinet room, for microorganism provides differential responses parameter, including but
It is not limited to：
A. by being turned on and off fluorescent lamp 3-1, visible ray illumination is controlled；
B. by being turned on and off uviol lamp 3-2 (uviol lamp can not simultaneously be opened with fluorescent lamp), ultraviolet radioactive is controlled；
C. by being turned on and off fan 4, the gas disturbance situation in control cabinet room；
D. cabin indoor temperature is controlled by changing ambient temperature, reading can be carried out by Hygrothermograph 5；
E. by back up ventilation mouthful 7 to the water vapour that atomization is passed through in cabin, cabin indoor humidity is changed, can be by Hygrothermograph
5 carry out reading；
F. by back up ventilation mouthfuls 7 to being passed through other gases, such as ozone (O in cabin3), enter in atmosphere with microorganism
Row reaction.
3) sampler is connected with gas outlet 6, the microorganism after collection reaction completion.Using BioStage or
Andersen samplers samples, can directly be cultivated, the concentration of Bacterial diversity in detection cabin.Use BioSampler
Samplers sample, obtains liquid sample, can carry out molecular Biological Detection, detects activity, oxidative stress and the drying of microorganism
Effect etc..
4) corpuscular counter is connected with gas outlet 6, can real-time detection cabin indoor accelerated test concentration.
Claims (10)
1. a kind of air microbe experiment consersion unit, including reaction cabin, empty gasification generator and air sampler, in reaction cabin
Side wall be provided with air inlet and be connected with empty gasification generator, relative opposite side wall is provided with gas outlet and air sampler
It is connected；Compartment roof is provided with fluorescent lamp and uviol lamp, and fan and Temperature Humidity Sensor are provided with cabin；Wherein：The reaction
The volume in cabin is 150-500 liters, and cabin inner walls scribble hydrophobic coating.
2. air microbe as claimed in claim 1 tests consersion unit, it is characterised in that the hydrophobic coating is silicon nanometer
Resin.
3. air microbe as claimed in claim 1 tests consersion unit, it is characterised in that the air generator produces gas
The microorganism of solation, is passed through in reaction cabin by air inlet；The air sampler is biological aerosol sampler, and collection is anti-
Microorganism after answering.
4. air microbe as claimed in claim 1 experiment consersion unit, it is characterised in that the empty gasification generator with enter
Drying tube is provided between gas port.
5. air microbe as claimed in claim 1 tests consersion unit, it is characterised in that the equipment is also included for real
When detection cabin in microbe granular concentration concentration detection apparatus, the gas outlet phase of the concentration detection apparatus and the reaction cabin
Even.
6. air microbe as claimed in claim 5 tests consersion unit, it is characterised in that the concentration detection apparatus are light
Learn corpuscular counter or ultraviolet-aerosol particle size analyzer.
7. air microbe as claimed in claim 1 tests consersion unit, it is characterised in that the fan is ceiling fan or ventilation
Fan.
8. air microbe as claimed in claim 1 tests consersion unit, it is characterised in that the Temperature Humidity Sensor is to divide
Body formula sensor, its probe is located at cabin interior, and display pannel is located at outside cabin.
9. air microbe as claimed in claim 1 tests consersion unit, it is characterised in that also set in reaction cabin cabin bottom
It is equipped with blower fan or ventilation fan；And/or, the cabin side wall of reaction cabin is provided with back up ventilation mouthful.
10. the method for a kind of microorganisms reactions change in atmosphere, using the sky described in claim 1~9 any one
Gas Experiment on Microbiology consersion unit is carried out, and first passes through sky gasification generator and aerosolized microbe granular is passed through into reaction cabin
In, one or more in providing following reaction condition for microorganism in reaction cabin：Fluorescent lamp provides visible ray illumination, ultraviolet
Lamp provides ultraviolet radioactive, the gas disturbance situation in fan control cabin, the reaction temperature in Temperature Humidity Sensor control cabinet room
And humidity, back up ventilation mouth be passed through reaction needed for gas；Microorganism after the completion of reaction is adopted by gas outlet by air sampler
Collection, carries out coherent detection.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710028214.3A CN106769811A (en) | 2017-01-16 | 2017-01-16 | A kind of air microbe tests consersion unit and application |
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| CN201710028214.3A CN106769811A (en) | 2017-01-16 | 2017-01-16 | A kind of air microbe tests consersion unit and application |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107462555A (en) * | 2017-07-25 | 2017-12-12 | 苏州江南航天机电工业有限公司 | A kind of airborne microorganisms laser detection and alarm system |
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| CN113933217A (en) * | 2021-09-10 | 2022-01-14 | 广东工业大学 | Biological aerosol generating and balancing system and application thereof |
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