CN114133044A - Organic sewage treatment agent embedded with anaerobic microorganisms and preparation method thereof - Google Patents
Organic sewage treatment agent embedded with anaerobic microorganisms and preparation method thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/04—Surfactants, used as part of a formulation or alone
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Abstract
The invention discloses an organic sewage treatment agent embedded with anaerobic microorganisms and a preparation method thereof, wherein the sewage treatment agent is prepared from 3-4 parts of ethylene diamine tetraacetic acid, 6-8 parts of sodium nitrite, 3-4 parts of thiourea, 3-4 parts of acrolein and 3-4 parts of sodium hexametaphosphate according to parts by weight, the microbial preparation is prepared from anaerobic microorganisms, nutrient solution and an embedding material, and the anaerobic microorganisms are formed by mixing alternaria, acidophilic methanogens, sulfate reducing bacteria and acidophilic acetogenic bacteria. The organic sewage treatment agent prepared by the application is mainly used for reacting acrolein, sodium hexametaphosphate, ethylene diamine tetraacetic acid disodium and thiourea aiming at chemical plant wastewater, so that a polyacrylamide solution is prepared, and the obtained polyacrylamide solution can be used as a flocculating agent to adsorb calcium ions and magnesium ions in the organic sewage, so that the effect of sewage treatment can be achieved.
Description
Technical Field
The invention relates to the technical field of sewage treatment agents, in particular to an organic sewage treatment agent embedded with anaerobic microorganisms and a preparation method thereof.
Background
Propionic acid is a raw material of a common food preservative and mildew preventive on the market, sulfate is an essential raw material in normal industrial production, and because industrial equipment cannot be completely sealed, partial sulfate, propionic acid, lime milk and other substances can leak out, so that rainwater, sewage and underground water near a chemical plant contain sulfate, propionic acid and other organic matters.
Most organic matters in the sewage have certain toxicity, and the accumulated organic matters are strong and are not easy to decompose, so that the environment and the human health are seriously harmed, and the current sewage treatment agent on the market has no certain pertinence, so that the treatment effect achieved by the used sewage treatment agent is not obvious in certain specific environments, and therefore the organic sewage treatment agent for embedding anaerobic microorganisms and the preparation method thereof are particularly important.
Disclosure of Invention
The invention aims to provide an organic sewage treatment agent embedded with anaerobic microorganisms and a preparation method thereof, so as to solve the problems in the background technology.
In order to solve the technical problems, the invention provides the following technical scheme: an organic sewage treatment agent for embedding anaerobic microorganisms is prepared from a main sewage treatment agent and a microbial preparation;
the main sewage treatment agent comprises the following raw materials, by weight, 3-4 parts of disodium ethylene diamine tetraacetate, 6-8 parts of sodium nitrite, 3-4 parts of thiourea, 3-4 parts of acrolein and 3-4 parts of sodium hexametaphosphate;
the microbial preparation is prepared from anaerobic microorganisms, nutrient solution and embedding materials.
Further, the anaerobic microorganism is formed by mixing the bacillus muticus, the methanogen acidophilus, the sulfate reducing bacteria and the acetogen acidophilus.
Furthermore, the embedding material comprises, by weight, 30-40 parts of polyvinyl alcohol, 15-20 parts of sodium alginate, 1-2 parts of nano aluminum oxide, 2-3 parts of calcium chloride and 2-3 parts of ammonium sulfate.
Furthermore, the nutrient solution is prepared from L-ascorbic acid, sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate.
Further, the L-ascorbic acid is prepared from glucose, hydrogen, a catalyst and zymophyte.
Further, the catalyst is nickel.
A preparation method of an organic sewage treatment agent embedding anaerobic microorganisms comprises the following steps,
(1) preparing anaerobic microbial liquid: activating anaerobic microorganisms to prepare anaerobic microorganism liquid;
(2) domestication of anaerobic microorganisms: adding nickel powder into the obtained anaerobic microorganism liquid, adding concentrated sulfuric acid to adjust the pH value, and performing domestication to obtain domesticated anaerobic microorganisms;
the sewage treatment agent prepared by the application is mainly used for sewage containing sulfate radicals, propionic acid, calcium ions and other organic matters, and the pH value of the sewage is acidic, so that acidophilic anaerobic microorganisms are purposefully selected when anaerobic microorganism species are selected, the acidophilic anaerobic microorganisms can survive in the sewage environment, and propionic acid in the sewage is decomposed, and then the purpose of treating the sewage is achieved.
The application selects and uses Syntrophobacter woolinii, acidophilic methanogen, sulfate reducing bacteria and acidophilic acetogen, the strains can survive and play a role in an acid environment on the one hand, on the other hand, the added acidophilic acetogen can generate acetic acid and hydrogen when decomposing organic matters, the generated acetic acid and hydrogen can be used as nutrients to promote the growth of the acidophilic methanogen, and because a sewage treatment tank is closed, the acidophilic acetogen can continuously generate hydrogen, the hydrogen content in the sewage treatment tank is increased, the pressure is increased, the growth rate of the acidophilic acetogen can be reduced due to a lighter condition, safety accidents can be caused seriously under the condition, on the one hand, the acetic acid and the hydrogen can be consumed due to the addition of the acidophilic methanogen, the growth of the acidophilic acetogen is accelerated, on the other hand, the acidophilic acetogen can decompose the organic matters only under the condition that the acidophilic methanogen exists, thereby achieving the purpose of degrading organic matters in the sewage.
(3) Preparing a nutrient solution: dissolving glucose, adding a catalyst, heating to 80-85 ℃, placing in a high-pressure reaction kettle, introducing hydrogen, heating and pressurizing to 153-154 ℃ and 3.8-3.9MPa, adding alkali liquor, controlling pH, adding zymocyte, fermenting for 2-3 days at 29-30 ℃, adding sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate, and uniformly stirring to obtain a nutrient solution;
the method comprises the steps of carrying out hydrogenation reduction on glucose, then carrying out fermentation to obtain the L-ascorbic acid, wherein the obtained nutrient solution mainly comprises the L-ascorbic acid, the glucose, the nickel and the fermentation bacteria, and using the L-ascorbic acid as a nutrient substance, but the L-ascorbic acid has the characteristic of easy oxidation, so that the oxidation degree of the L-ascorbic acid is controlled in the preparation process.
The obtained L-ascorbic acid is mixed with sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate, wherein dipotassium hydrogen phosphate and ammonium dihydrogen phosphate can provide nitrogen, phosphorus and potassium for acidophilic methanogen, acidophilic acetogen can provide carbon source, so as to form basic survival conditions of the methanogen acidophilus, the application adds the L-ascorbic acid on the basis, the L-ascorbic acid can stimulate the growth of the methanogen acidophilus, and nickel is added as a catalyst during the preparation of the L-ascorbic acid, and is reserved as a trace element of the methanogen acidophilus after the reaction is finished, the existence of the nickel can promote the generation of catalase in the methanogen acidophilus and stimulate the growth of the methanogen acidophilus, and part of unreacted glucose can be used as nutrients to provide a carbon source for the strains, so that the capacity of the product for treating the organic sewage is improved.
However, the addition of nickel is not friendly to other anaerobic microorganisms, so that the microorganisms are domesticated before the microbial agent is prepared, and the other anaerobic microorganisms can be ensured to have certain nickel resistance.
(4) Preparation of microbial preparation: heating polyvinyl alcohol at 90-93 ℃, adding sodium alginate, stirring, uniformly stirring, adding nano-alumina for ultrasonic modification, adding the obtained nutrient solution and the domesticated anaerobic microorganism, adding calcium chloride and saturated ammonium sulfate solution, and granulating to obtain a microbial preparation;
this application mixes polyvinyl alcohol and ammonium alginate, add nanometer aluminium oxide and carry out the ultrasonic modification to it, and then obtained modified polyvinyl alcohol, the microbial preparation through modified polyvinyl alcohol embedding has comparatively excellent mechanical properties, and modified polyvinyl alcohol has certain removal effect to the dissolved oxygen content and the ammonia nitrogen of sewage, the dissolved oxygen content that reduces in the sewage can provide good living environment for anaerobic microorganism, increase the activity of enzyme among the anaerobic microorganism, and then improve the sewage treatment effect.
(5) Preparing a main sewage treatment agent: mixing ethylene diamine tetraacetic acid, sodium nitrite and thiourea, heating after uniformly stirring, adding acrolein, sodium carbonate and sodium hexametaphosphate, and uniformly stirring to obtain a main sewage treatment agent;
(6) preparing a sewage treatment agent: and adding the obtained microbial preparation into the obtained main sewage treatment agent, and uniformly stirring to obtain the sewage treatment agent.
(7) And (4) placing the sewage treatment agent in a sewage treatment tank for sewage treatment.
Furthermore, the sewage treatment agent is used at the temperature of 25-30 ℃.
Further, the sewage treatment tank is a sealing device, and the top of the sewage treatment tank is provided with a gas return line which is connected with an inlet of the sewage treatment tank.
The application uses anaerobic microorganisms to treat organic pollutants and possibly causes secondary pollution because the sewage of a factory contains a large amount of sulfate, hydrogen sulfide gas can be generated through the decomposition of sulfate reducing bacteria in the anaerobic environment, the hydrogen sulfide is toxic irritant gas, and therefore certain requirements are required on the air tightness of the sewage treatment tank, and the hydrogen sulfide gas is prevented from being leaked to cause harm to the environment and a human body.
The top of the sewage treatment tank used in the application is provided with a gas return line and is connected to a sewage inlet of the sewage treatment tank, the reason is that L-ascorbic acid is used in the application, L-ascorbic acid can be oxidized in the preparation and use processes to form dehydroascorbic acid, the growth rate of anaerobic microorganisms can be greatly reduced by the production of the dehydroascorbic acid, and further the sewage treatment rate is reduced, so hydrogen sulfide is recycled in the application, the hydrogen sulfide is stronger in the sewage treated in the application, introduced into the sewage inlet, mixed with flowing organic sewage, contacted with a microbial agent, and further partial dehydroascorbic acid can be reduced, and further the growth rate of the anaerobic microorganisms is ensured, and the sewage treatment rate of products is ensured.
Furthermore, nitrogen is introduced into the sewage treatment tank to ensure that the sewage treatment tank is in a low-oxygen content state.
Compared with the prior art, the invention has the following beneficial effects: the organic sewage treatment agent of this application preparation is mainly to chemical plant waste water, this chemical plant owner produces propionic acid, in the industrial production that propionic acid was prepared, the sulfate is essential raw materials, because industrial equipment can not totally be airtight, can make partial sulfate, propionic acid, substances such as lime milk leak, lead to near rainwater of this chemical plant, sewage and groundwater contain the sulfate, propionic acid and other organic matters, consequently this application uses acrolein, sodium hexametaphosphate, disodium ethylenediamine tetraacetic acid and thiourea reaction, and then prepare polyacrylamide solution, polyacrylamide solution that obtains is at first can be with the calcium ion in the organic sewage as the flocculating agent, magnesium ion adsorbs, degrade, and then can reach sewage treatment's effect.
Microbial preparation has been added to this application, mainly carries out the embedding through modified polyvinyl alcohol to anaerobic microorganisms and nutrient substance among the anaerobic microorganisms that add, and then has obtained microbial preparation. The added microbial preparation can degrade propionic acid and sulfate ions in the sewage to further obtain methane and hydrogen sulfide gas, so that the sewage treatment tank has sealing property.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
An organic sewage treatment agent for embedding anaerobic microorganisms is prepared from a main sewage treatment agent and a microbial preparation;
the main sewage treatment agent comprises the following raw materials, by weight, 3 parts of disodium ethylene diamine tetraacetate, 6 parts of sodium nitrite, 3 parts of thiourea, 3 parts of acrolein and 3 parts of sodium hexametaphosphate;
the microbial preparation is prepared from anaerobic microorganisms, nutrient solution and embedding materials.
The anaerobic microorganism is formed by mixing syntrophic bacillus, acidophilic methanogen, sulfate reducing bacteria and acidophilic acetogen.
The embedding material comprises the following raw materials, by weight, 30 parts of polyvinyl alcohol, 15 parts of sodium alginate, 1 part of nano aluminum oxide, 2 parts of calcium chloride and 2 parts of ammonium sulfate.
The nutrient solution is prepared from L-ascorbic acid, sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate.
The L-ascorbic acid is prepared from glucose, hydrogen, a catalyst and zymocyte.
The catalyst is nickel.
A preparation method of an organic sewage treatment agent embedding anaerobic microorganisms comprises the following steps,
(1) preparing anaerobic microbial liquid: activating anaerobic microorganisms to prepare anaerobic microorganism liquid;
(2) domestication of anaerobic microorganisms: adding nickel powder into the obtained anaerobic microorganism liquid, adding concentrated sulfuric acid to adjust the pH value, and performing domestication to obtain domesticated anaerobic microorganisms;
(3) preparing a nutrient solution: dissolving glucose, adding a catalyst, heating to 80 ℃, placing in a high-pressure reaction kettle, introducing hydrogen, heating and pressurizing to 153 ℃ and 3.8MPa, adding alkali liquor, controlling the pH, adding zymocyte, fermenting at 29 ℃ for 2 days, adding sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate, and uniformly stirring to obtain a nutrient solution;
(4) preparation of microbial preparation: heating polyvinyl alcohol at 90 ℃, adding sodium alginate, stirring, adding nano-alumina after uniformly stirring for ultrasonic modification, adding the obtained nutrient solution and microorganisms, adding calcium chloride and saturated ammonium sulfate solution, and granulating to obtain a microbial preparation;
(5) preparing a main sewage treatment agent: mixing ethylene diamine tetraacetic acid, sodium nitrite and thiourea, heating after uniformly stirring, adding acrolein, sodium carbonate and sodium hexametaphosphate, and uniformly stirring to obtain a main sewage treatment agent;
(6) preparing a sewage treatment agent: adding the obtained microbial preparation into the obtained main sewage treatment agent, and uniformly stirring to obtain a sewage treatment agent;
(7) and (3) placing a sewage treatment agent in a sewage treatment tank, wherein the temperature of the sewage treatment tank is 25 ℃, the sewage treatment tank is sealing equipment, and the top of the sewage treatment tank is provided with a gas return line which is connected with an inlet of the sewage treatment tank for sewage treatment.
Example 2
An organic sewage treatment agent for embedding anaerobic microorganisms is prepared from a main sewage treatment agent and a microbial preparation;
the main sewage treatment agent comprises the following raw materials, by weight, 4 parts of disodium ethylene diamine tetraacetate, 7 parts of sodium nitrite, 3.5 parts of thiourea, 3.5 parts of acrolein and 3.5 parts of sodium hexametaphosphate;
the microbial preparation is prepared from anaerobic microorganisms, nutrient solution and embedding materials.
The anaerobic microorganism is formed by mixing syntrophic bacillus, acidophilic methanogen, sulfate reducing bacteria and acidophilic acetogen.
The embedding material comprises the following raw materials, by weight, 35 parts of polyvinyl alcohol, 17 parts of sodium alginate, 1.5 parts of nano aluminum oxide, 2.5 parts of calcium chloride and 2.5 parts of ammonium sulfate.
The nutrient solution is prepared from L-ascorbic acid, sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate.
The L-ascorbic acid is prepared from glucose, hydrogen, a catalyst and zymocyte.
The catalyst is nickel.
A preparation method of an organic sewage treatment agent embedding anaerobic microorganisms comprises the following steps,
(1) preparing anaerobic microbial liquid: activating anaerobic microorganisms to prepare anaerobic microorganism liquid;
(2) domestication of anaerobic microorganisms: adding nickel powder into the obtained anaerobic microorganism liquid, adding concentrated sulfuric acid to adjust the pH value, and performing domestication to obtain domesticated anaerobic microorganisms;
(3) preparing a nutrient solution: dissolving glucose, adding a catalyst, heating to 83 ℃, placing in a high-pressure reaction kettle, introducing hydrogen, heating and pressurizing to 153.5 ℃ and 3.85MPa, adding alkali liquor, controlling the pH, adding zymocyte, fermenting for 2.5 days at 29.5 ℃, adding sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate, and uniformly stirring to obtain a nutrient solution;
(4) preparation of microbial preparation: heating polyvinyl alcohol at 92 ℃, adding sodium alginate, stirring, adding nano-alumina after uniformly stirring for ultrasonic modification, adding the obtained nutrient solution and microorganisms, adding calcium chloride and saturated ammonium sulfate solution, and granulating to obtain a microbial preparation;
(5) preparing a main sewage treatment agent: mixing ethylene diamine tetraacetic acid, sodium nitrite and thiourea, heating after uniformly stirring, adding acrolein, sodium carbonate and sodium hexametaphosphate, and uniformly stirring to obtain a main sewage treatment agent;
(6) preparing a sewage treatment agent: adding the obtained microbial preparation into the obtained main sewage treatment agent, and uniformly stirring to obtain a sewage treatment agent;
(7) and (3) placing a sewage treatment agent in a sewage treatment tank, wherein the temperature of the sewage treatment tank is 27 ℃, the sewage treatment tank is sealing equipment, and the top of the sewage treatment tank is provided with a gas return line which is connected with an inlet of the sewage treatment tank for sewage treatment.
Example 3
An organic sewage treatment agent for embedding anaerobic microorganisms is prepared from a main sewage treatment agent and a microbial preparation;
the main sewage treatment agent comprises the following raw materials, by weight, 4 parts of disodium ethylene diamine tetraacetate, 8 parts of sodium nitrite, 4 parts of thiourea, 4 parts of acrolein and 4 parts of sodium hexametaphosphate;
the microbial preparation is prepared from anaerobic microorganisms, nutrient solution and embedding materials.
The anaerobic microorganism is formed by mixing syntrophic bacillus, acidophilic methanogen, sulfate reducing bacteria and acidophilic acetogen.
The embedding material comprises the following raw materials, by weight, 40 parts of polyvinyl alcohol, 15-20 parts of sodium alginate, 2 parts of nano aluminum oxide, 3 parts of calcium chloride and 3 parts of ammonium sulfate.
The nutrient solution is prepared from L-ascorbic acid, sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate.
The L-ascorbic acid is prepared from glucose, hydrogen, a catalyst and zymocyte.
The catalyst is nickel.
A preparation method of an organic sewage treatment agent embedding anaerobic microorganisms comprises the following steps,
(1) preparing anaerobic microbial liquid: activating anaerobic microorganisms to prepare anaerobic microorganism liquid;
(2) domestication of anaerobic microorganisms: adding nickel powder into the obtained anaerobic microorganism liquid, adding concentrated sulfuric acid to adjust the pH value, and performing domestication to obtain domesticated anaerobic microorganisms;
(3) preparing a nutrient solution: dissolving glucose, adding a catalyst, heating to 85 ℃, placing in a high-pressure reaction kettle, introducing hydrogen, heating and pressurizing to 154 ℃ and 3.9MPa, adding alkali liquor, controlling the pH, adding zymocyte, fermenting at 30 ℃ for 3 days, adding sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate, and uniformly stirring to obtain a nutrient solution;
(4) preparation of microbial preparation: heating polyvinyl alcohol at the temperature of 93 ℃, adding sodium alginate, stirring, adding nano-alumina after uniformly stirring for ultrasonic modification, adding the obtained nutrient solution and microorganisms, adding calcium chloride and saturated ammonium sulfate solution, and granulating to obtain a microbial preparation;
(5) preparing a main sewage treatment agent: mixing ethylene diamine tetraacetic acid, sodium nitrite and thiourea, heating after uniformly stirring, adding acrolein, sodium carbonate and sodium hexametaphosphate, and uniformly stirring to obtain a main sewage treatment agent;
(6) preparing a sewage treatment agent: adding the obtained microbial preparation into the obtained main sewage treatment agent, and uniformly stirring to obtain a sewage treatment agent;
(7) and (3) placing the sewage treatment agent in a sewage treatment tank, wherein the temperature of the sewage treatment tank is 30 ℃, the sewage treatment tank is sealing equipment, and the top of the sewage treatment tank is provided with a gas return line which is connected with an inlet of the sewage treatment tank for sewage treatment.
Comparative example 1
The organic sewage treatment agent comprises the following raw materials, by weight, 4 parts of disodium ethylene diamine tetraacetate, 8 parts of sodium nitrite, 4 parts of thiourea, 4 parts of acrolein and 4 parts of sodium hexametaphosphate;
a preparation method of an organic sewage treating agent comprises the following steps,
mixing ethylene diamine tetraacetic acid, sodium nitrite and thiourea, heating after uniformly stirring, adding acrolein, sodium carbonate and sodium hexametaphosphate, and uniformly stirring to obtain a sewage treatment agent;
and (3) placing the sewage treatment agent in a sewage treatment tank, wherein the temperature of the sewage treatment tank is 30 ℃, the sewage treatment tank is sealing equipment, and the top of the sewage treatment tank is provided with a gas return line which is connected with an inlet of the sewage treatment tank for sewage treatment.
Comparative example 2
An organic sewage treatment agent for embedding anaerobic microorganisms is prepared from a main sewage treatment agent and a microbial preparation;
the main sewage treatment agent comprises the following raw materials, by weight, 4 parts of disodium ethylene diamine tetraacetate, 8 parts of sodium nitrite, 4 parts of thiourea, 4 parts of acrolein and 4 parts of sodium hexametaphosphate;
the microbial preparation is prepared from anaerobic microorganisms, nutrient solution and embedding materials.
The anaerobic microorganism is formed by mixing syntrophic bacillus, acidophilic methanogen, sulfate reducing bacteria and acidophilic acetogen.
The embedding material comprises the following raw materials, by weight, 40 parts of polyvinyl alcohol, 15-20 parts of sodium alginate, 2 parts of nano aluminum oxide, 3 parts of calcium chloride and 3 parts of ammonium sulfate.
The nutrient solution is prepared from sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate.
A preparation method of an organic sewage treatment agent embedding anaerobic microorganisms comprises the following steps,
(1) preparing anaerobic microbial liquid: activating anaerobic microorganisms to prepare anaerobic microorganism liquid;
(2) domestication of anaerobic microorganisms: adding nickel powder into the obtained anaerobic microorganism liquid, adding concentrated sulfuric acid to adjust the pH value, and performing domestication to obtain domesticated anaerobic microorganisms;
(3) preparing a nutrient solution: mixing and stirring sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate, and uniformly stirring to obtain a nutrient solution;
(4) preparation of microbial preparation: heating polyvinyl alcohol at the temperature of 93 ℃, adding sodium alginate, stirring, adding nano-alumina after uniformly stirring for ultrasonic modification, adding the obtained nutrient solution and microorganisms, adding calcium chloride and saturated ammonium sulfate solution, and granulating to obtain a microbial preparation;
(5) preparing a main sewage treatment agent: mixing ethylene diamine tetraacetic acid, sodium nitrite and thiourea, heating after uniformly stirring, adding acrolein, sodium carbonate and sodium hexametaphosphate, and uniformly stirring to obtain a main sewage treatment agent;
(6) preparing a sewage treatment agent: adding the obtained microbial preparation into the obtained main sewage treatment agent, and uniformly stirring to obtain a sewage treatment agent;
(7) and (3) placing the sewage treatment agent in a sewage treatment tank, wherein the temperature of the sewage treatment tank is 30 ℃, the sewage treatment tank is sealing equipment, and the top of the sewage treatment tank is provided with a gas return line which is connected with an inlet of the sewage treatment tank for sewage treatment.
Comparative example 3
An organic sewage treatment agent for embedding anaerobic microorganisms is prepared from a main sewage treatment agent and a microbial preparation;
the main sewage treatment agent comprises the following raw materials, by weight, 4 parts of disodium ethylene diamine tetraacetate, 8 parts of sodium nitrite, 4 parts of thiourea, 4 parts of acrolein and 4 parts of sodium hexametaphosphate;
the microbial preparation is prepared from anaerobic microorganisms, nutrient solution and embedding materials.
The anaerobic microorganism is formed by mixing syntrophic bacillus, acidophilic methanogen, sulfate reducing bacteria and acidophilic acetogen.
The embedding material comprises the following raw materials, by weight, 40 parts of polyvinyl alcohol, 15-20 parts of sodium alginate, 2 parts of nano aluminum oxide, 3 parts of calcium chloride and 3 parts of ammonium sulfate.
The nutrient solution is prepared from L-ascorbic acid, sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate.
The L-ascorbic acid is prepared from glucose, hydrogen, a catalyst and zymocyte.
The catalyst is nickel.
A preparation method of an organic sewage treatment agent embedding anaerobic microorganisms comprises the following steps,
(1) preparing anaerobic microbial liquid: activating anaerobic microorganisms to prepare anaerobic microorganism liquid;
(2) domestication of anaerobic microorganisms: adding nickel powder into the obtained anaerobic microorganism liquid, adding concentrated sulfuric acid to adjust the pH value, and performing domestication to obtain domesticated anaerobic microorganisms;
(3) preparing a nutrient solution: dissolving glucose, adding a catalyst, heating to 85 ℃, placing in a high-pressure reaction kettle, introducing hydrogen, heating and pressurizing to 154 ℃ and 3.9MPa, adding alkali liquor, controlling the pH, adding zymocyte, fermenting at 30 ℃ for 3 days, adding sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate, and uniformly stirring to obtain a nutrient solution;
(4) preparation of microbial preparation: heating polyvinyl alcohol at the temperature of 93 ℃, adding sodium alginate, stirring, adding nano-alumina after uniformly stirring for ultrasonic modification, adding the obtained nutrient solution and microorganisms, adding calcium chloride and saturated ammonium sulfate solution, and granulating to obtain a microbial preparation;
(5) preparing a main sewage treatment agent: mixing ethylene diamine tetraacetic acid, sodium nitrite and thiourea, heating after uniformly stirring, adding acrolein, sodium carbonate and sodium hexametaphosphate, and uniformly stirring to obtain a main sewage treatment agent;
(6) preparing a sewage treatment agent: adding the obtained microbial preparation into the obtained main sewage treatment agent, and uniformly stirring to obtain a sewage treatment agent;
(7) and (3) placing the sewage treatment agent in a sewage treatment tank, wherein the temperature of the sewage treatment tank is 30 ℃, and the sewage treatment tank is a sealed device for sewage treatment.
Comparative example 4
An organic sewage treatment agent for embedding anaerobic microorganisms is prepared from a main sewage treatment agent and a microbial preparation;
the main sewage treatment agent comprises the following raw materials, by weight, 4 parts of disodium ethylene diamine tetraacetate, 8 parts of sodium nitrite, 4 parts of thiourea, 4 parts of acrolein and 4 parts of sodium hexametaphosphate;
the microbial preparation is prepared from anaerobic microorganisms, nutrient solution and embedding materials.
The embedding material comprises the following raw materials, by weight, 40 parts of polyvinyl alcohol, 15-20 parts of sodium alginate, 2 parts of nano aluminum oxide, 3 parts of calcium chloride and 3 parts of ammonium sulfate.
The nutrient solution is prepared from L-ascorbic acid, sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate.
The L-ascorbic acid is prepared from glucose, hydrogen, a catalyst and zymocyte.
The catalyst is nickel.
A preparation method of an organic sewage treatment agent embedding anaerobic microorganisms comprises the following steps,
(1) preparing anaerobic microbial liquid: activating anaerobic microorganisms to prepare anaerobic microorganism liquid;
(2) domestication of anaerobic microorganisms: adding nickel powder into the obtained anaerobic microorganism liquid, adding concentrated sulfuric acid to adjust the pH value, and performing domestication to obtain domesticated anaerobic microorganisms;
(3) preparing a nutrient solution: dissolving glucose, adding a catalyst, heating to 85 ℃, placing in a high-pressure reaction kettle, introducing hydrogen, heating and pressurizing to 154 ℃ and 3.9MPa, adding alkali liquor, controlling the pH, adding zymocyte, fermenting at 30 ℃ for 3 days, adding sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate, and uniformly stirring to obtain a nutrient solution;
(4) preparation of microbial preparation: heating polyvinyl alcohol at the temperature of 93 ℃, adding sodium alginate, stirring, adding nano-alumina after uniformly stirring for ultrasonic modification, adding the obtained nutrient solution and microorganisms, adding calcium chloride and saturated ammonium sulfate solution, and granulating to obtain a microbial preparation;
(5) preparing a main sewage treatment agent: mixing ethylene diamine tetraacetic acid, sodium nitrite and thiourea, heating after uniformly stirring, adding acrolein, sodium carbonate and sodium hexametaphosphate, and uniformly stirring to obtain a main sewage treatment agent;
(6) preparing a sewage treatment agent: adding the obtained microbial preparation into the obtained main sewage treatment agent, and uniformly stirring to obtain a sewage treatment agent;
(7) and (3) placing the sewage treatment agent in a sewage treatment tank, wherein the temperature of the sewage treatment tank is 30 ℃, the sewage treatment tank is sealing equipment, and the top of the sewage treatment tank is provided with a gas return line which is connected with an inlet of the sewage treatment tank for sewage treatment.
Comparative example 5
An organic sewage treatment agent for embedding anaerobic microorganisms is prepared from a main sewage treatment agent and a microbial preparation;
the main sewage treatment agent comprises the following raw materials, by weight, 4 parts of disodium ethylene diamine tetraacetate, 8 parts of sodium nitrite, 4 parts of thiourea, 4 parts of acrolein and 4 parts of sodium hexametaphosphate;
the microbial preparation is prepared from anaerobic microorganisms, nutrient solution and embedding materials.
The anaerobic microorganism is formed by mixing syntrophic bacillus, acidophilic methanogen, sulfate reducing bacteria and acidophilic acetogen.
The embedding material comprises the following raw materials, by weight, 40 parts of polyvinyl alcohol, 3 parts of calcium chloride and 3 parts of ammonium sulfate.
The nutrient solution is prepared from L-ascorbic acid, sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate.
The L-ascorbic acid is prepared from glucose, hydrogen, a catalyst and zymocyte.
The catalyst is nickel.
A preparation method of an organic sewage treatment agent embedding anaerobic microorganisms comprises the following steps,
(1) preparing anaerobic microbial liquid: activating anaerobic microorganisms to prepare anaerobic microorganism liquid;
(2) domestication of anaerobic microorganisms: adding nickel powder into the obtained anaerobic microorganism liquid, adding concentrated sulfuric acid to adjust the pH value, and performing domestication to obtain domesticated anaerobic microorganisms;
(3) preparing a nutrient solution: dissolving glucose, adding a catalyst, heating to 85 ℃, placing in a high-pressure reaction kettle, introducing hydrogen, heating and pressurizing to 154 ℃ and 3.9MPa, adding alkali liquor, controlling the pH, adding zymocyte, fermenting at 30 ℃ for 3 days, adding sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate, and uniformly stirring to obtain a nutrient solution;
(4) preparation of microbial preparation: heating polyvinyl alcohol at 93 deg.C, adding the obtained nutrient solution and microorganism, adding calcium chloride and saturated ammonium sulfate solution, and granulating to obtain microbial preparation;
(5) preparing a main sewage treatment agent: mixing ethylene diamine tetraacetic acid, sodium nitrite and thiourea, heating after uniformly stirring, adding acrolein, sodium carbonate and sodium hexametaphosphate, and uniformly stirring to obtain a main sewage treatment agent;
(6) preparing a sewage treatment agent: adding the obtained microbial preparation into the obtained main sewage treatment agent, and uniformly stirring to obtain a sewage treatment agent;
(7) and (3) placing the sewage treatment agent in a sewage treatment tank, wherein the temperature of the sewage treatment tank is 30 ℃, the sewage treatment tank is sealing equipment, and the top of the sewage treatment tank is provided with a gas return line which is connected with an inlet of the sewage treatment tank for sewage treatment.
Experiment of
Comparative examples 1, 2, 3, 4 and 5 were set up with example 3 as a control, in which comparative example 1 was carried out without adding the microbial preparation, comparative example 2 was carried out without adding L-ascorbic acid, comparative example 3 was carried out without a gas reflux route, comparative example 4 was carried out with a conventional microbial preparation, and comparative example 5 was carried out with polyvinyl alcohol.
The samples of example 1, example 2, example 3, comparative example 1, comparative example 4 and comparative example 5 were put into a sewage tank, sewage was introduced, the contents of SS (suspended substances) and organic substances in raw water and effluent were analyzed, and the removal rates of SS and organic substances were calculated, and the results were as follows,
watch 1
The samples of example 1, example 2, example 3, comparative example 2 and comparative example 3 were subjected to a long-term use test, and as a result,
watch two
The reason why the microbial preparation was not added in comparative example 1 resulted in the decrease of the treatment ability of comparative example 1 for propionic acid and suspended matter in sewage is that various anaerobic microorganisms were mainly contained in the biological preparation and the anaerobic microorganisms were able to decompose propionic acid in sewage, and the change of the treatment ability of the suspension was caused by the effect of the modified polyvinyl alcohol as the embedding material in examples 1, 2 and 3.
The reason why the number of total viable bacteria in the use process of the comparative example 2 is smaller than that in the practical examples 1, 2 and 3 because the L-ascorbic acid and the nickel are added in the practical examples 1, 2 and 3 is that the nutrient content is richer, the growth of methanogens can be accelerated, and the content of microorganisms in the treating agent is further ensured.
In comparative example 3, a gas reflux route is not provided, and although L-ascorbic acid and nickel are added in comparative example 3, since part of L-ascorbic acid is inevitably oxidized during the preparation process and the use process, the growth rate of the microorganism in comparative example 1 is low relative to that of examples 1, 2 and 3, thereby causing a decrease in the content of the microorganism in comparative example 3.
The conventional microorganism preparation used in comparative example 4 results in a reduction in the removal rate of suspended matters and organic matters in comparative example 4 compared to examples 1, 3, and examples, because the present application is mainly directed to chemical plant industrial wastewater accompanied by propionic acid and having an acidic pH, and has a certain pertinence in the selection of microorganisms, and thus a higher removal rate of organic matters.
The polyvinyl alcohol used in comparative example 5 has a lower removal rate of suspended substances and organic substances than those in examples 1, 3 and 3, because the polyvinyl alcohol is modified in examples 1, 3 and 3, and the modified polyvinyl alcohol has higher stability than that of the conventional polyvinyl alcohol, thereby creating a good environment for the survival of microorganisms.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. An organic sewage treatment agent for embedding anaerobic microorganisms is characterized in that: the sewage treatment agent is prepared from a main sewage treatment agent and a microbial preparation;
the main sewage treatment agent comprises the following raw materials, by weight, 3-4 parts of disodium ethylene diamine tetraacetate, 6-8 parts of sodium nitrite, 3-4 parts of thiourea, 3-4 parts of acrolein and 3-4 parts of sodium hexametaphosphate;
the microbial preparation is prepared from anaerobic microorganisms, nutrient solution and embedding materials; the anaerobic microorganism is formed by mixing syntrophic bacillus, acidophilic methanogen, sulfate reducing bacteria and acidophilic acetogen.
2. The organic sewage treatment agent embedding anaerobic microorganisms according to claim 1, wherein: the embedding material comprises, by weight, 30-40 parts of polyvinyl alcohol, 15-20 parts of sodium alginate, 1-2 parts of nano aluminum oxide, 2-3 parts of calcium chloride and 2-3 parts of ammonium sulfate.
3. The organic sewage treatment agent embedding anaerobic microorganisms according to claim 1, wherein: the nutrient solution is prepared from L-ascorbic acid, sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate.
4. The organic sewage treatment agent embedding anaerobic microorganisms according to claim 3, wherein: the L-ascorbic acid is prepared from glucose, hydrogen, a catalyst and zymocyte.
5. The organic sewage treatment agent embedding anaerobic microorganisms according to claim 4, wherein: the catalyst is nickel.
6. A preparation method of an organic sewage treatment agent embedded with anaerobic microorganisms is characterized by comprising the following steps: the steps are as follows,
(1) preparing anaerobic microbial liquid: activating anaerobic microorganisms to prepare anaerobic microorganism liquid;
(2) domestication of anaerobic microorganisms: adding nickel powder into the obtained anaerobic microorganism liquid, adding concentrated sulfuric acid to adjust the pH value, and performing domestication to obtain domesticated anaerobic microorganisms;
(3) preparing a nutrient solution: dissolving glucose, adding a catalyst, heating to 80-85 ℃, placing in a high-pressure reaction kettle, introducing hydrogen, heating and pressurizing to 153-154 ℃ and 3.8-3.9MPa, adding alkali liquor, controlling pH, adding zymocyte, fermenting for 2-3 days at 29-30 ℃, adding sodium chloride, dipotassium hydrogen phosphate and ammonium dihydrogen phosphate, and uniformly stirring to obtain a nutrient solution;
(4) preparation of microbial preparation: heating polyvinyl alcohol at 90-93 ℃, adding sodium alginate, stirring, uniformly stirring, adding nano-alumina for ultrasonic modification, adding the obtained nutrient solution and the domesticated anaerobic microorganism, adding calcium chloride and saturated ammonium sulfate solution, and granulating to obtain a microbial preparation;
(5) preparing a main sewage treatment agent: mixing ethylene diamine tetraacetic acid, sodium nitrite and thiourea, heating after uniformly stirring, adding acrolein, sodium carbonate and sodium hexametaphosphate, and uniformly stirring to obtain a main sewage treatment agent;
(6) preparing a sewage treatment agent: and adding the obtained microbial preparation into the obtained main sewage treatment agent, and uniformly stirring to obtain the sewage treatment agent.
7. The method for preparing an anaerobic microorganism-embedded organic sewage treatment agent according to claim 6, wherein: when the sewage treatment agent is used for sewage treatment, nitrogen is introduced and the sewage treatment is carried out in a closed environment, the temperature of the sewage treatment is 25-30 ℃, and hydrogen sulfide gas generated in the treatment process flows back to a sewage inlet.
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