CN114133029B - Fungus-rich porous denitrification filler and preparation method thereof - Google Patents
Fungus-rich porous denitrification filler and preparation method thereof Download PDFInfo
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- CPLXHLVBOLITMK-UHFFFAOYSA-N Magnesium oxide Chemical compound [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 claims description 20
- 238000009630 liquid culture Methods 0.000 claims description 17
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 15
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 14
- 238000001354 calcination Methods 0.000 claims description 13
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 239000005995 Aluminium silicate Substances 0.000 claims description 10
- 239000004115 Sodium Silicate Substances 0.000 claims description 10
- 235000012211 aluminium silicate Nutrition 0.000 claims description 10
- 239000004927 clay Substances 0.000 claims description 10
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims description 10
- 239000000395 magnesium oxide Substances 0.000 claims description 10
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims description 10
- 229910052911 sodium silicate Inorganic materials 0.000 claims description 10
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- 239000008187 granular material Substances 0.000 claims description 8
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- 239000007787 solid Substances 0.000 claims description 7
- 229940041514 candida albicans extract Drugs 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 239000012137 tryptone Substances 0.000 claims description 6
- 239000012138 yeast extract Substances 0.000 claims description 6
- 230000003100 immobilizing effect Effects 0.000 claims description 5
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims description 4
- 230000004913 activation Effects 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
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- 230000008014 freezing Effects 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 32
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 16
- 239000010865 sewage Substances 0.000 abstract description 16
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- 244000005700 microbiome Species 0.000 description 14
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- 230000009286 beneficial effect Effects 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F2003/001—Biological treatment of water, waste water, or sewage using granular carriers or supports for the microorganisms
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biological Treatment Of Waste Water (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a bacteria-rich porous denitrification filler and a preparation method thereof. The filler has high porosity and large specific surface area, the internal porous structure is rich in a large amount of denitrifying bacteria, the biological activity is high, and the uniform and dense porous structure can provide perfect habitat and attachment points for the denitrifying bacteria, so that the denitrifying bacteria can be effectively fixed, and the binding force and stability are good. The preparation method comprises the following steps: preparing a granular porous carrier, preparing denitrifying bacteria fermentation liquor and fixing denitrifying bacteria to the granular porous carrier. The filler is applied to the denitrification biological filter, and a large amount of denitrification bacteria are fixed on the filler, so that the denitrification effect can be rapidly exerted, the stability is strong, the film forming time is saved, the denitrification rate in the reactor can be effectively improved, the treatment capacity of the reactor is enhanced, and the filler has important significance for improving the problem that the total nitrogen of a sewage treatment plant does not reach the standard.
Description
Technical Field
The invention relates to the field of sewage microorganism treatment, in particular to a bacterial porous denitrification filler and a preparation method thereof.
Background
In recent years, along with the rapid development of urban land in China, the problem of water pollution is increasingly serious. The requirements for the quality of effluent from sewage treatment plants are also gradually increasing, and many areas begin to execute the first-level A standard of effluent or the local standard which is more strict than the first-level A standard. Especially total nitrogen, is an important conventional index for urban sewage treatment, and has been the focus of research. The existing urban sewage treatment plants in China basically adopt an activated sludge method, namely a nitrification and denitrification treatment process for removing total nitrogen, wherein the denitrification process controls NO 3 - -N gradually changes to N 2 Is a key step in removing nitrogen from a body of water. This process is catalyzed by heterotrophic denitrifying bacteria under anoxic conditions. However, in actual operation, the total nitrogen removal effect is not ideal due to various influences of complex water quality of the inlet water, high concentration of pollutants, low water temperature in winter and the like, and the problem that total nitrogen does not reach standards exists in many sewage treatment plants.
The denitrification biological filter is widely accepted due to low investment, simple structure, large biomass and high treatment efficiency, and gradually becomes a novel sewage advanced denitrification treatment technology. The technology is characterized in that the existing denitrification biological filter adopts quartz sand with low manufacturing cost as a filler, bacteria gradually accumulate on the surface of the quartz sand by taking the quartz sand as a film-forming medium, and the denitrification bacteria in sewage are fixed while forming a biological film, so that the denitrification effect is exerted to degrade the total nitrogen in the sewage. However, the denitrifying bacteria in the sewage have quite small proportion and need to be accumulated slowly to a certain amount to play a role, so that the film hanging is a slow process, usually about 2 weeks are required from the starting of the equipment to the stabilization of the water outlet, and even the film hanging is unsuccessful due to the influence of the water quality of the water inlet. In addition, other heterotrophic microorganisms can be greatly propagated while film formation is carried out, and the heterotrophic microorganisms compete with denitrifying bacteria for carbon sources to form a certain competition relationship, so that the denitrifying efficiency is affected.
The immobilized microorganism technology is a bio-enhancement technology which utilizes physical or chemical means to immobilize microorganisms in a specific space, so that the microorganisms are highly dense and maintain inherent biological activity, and can rapidly proliferate and act under the condition of proper environment. The denitrifying bacteria are prepared into a specific bacterial product in a certain form by utilizing an immobilized microorganism technology and are added into a reactor of a sewage treatment plant, so that the number of denitrifying bacteria in the reactor can be effectively increased, the denitrification efficiency is improved, and the treatment capacity of the reactor is further improved. The immobilized microorganism technology mainly comprises four methods: adsorption, embedding, crosslinking and self-immobilization. The adsorption method is a method of forming a biofilm by immobilizing microorganisms on the surface or inside of a carrier using physical action between the microorganisms and an adsorption carrier. The method is widely applied due to simple preparation, low cost and high biological activity, but the method also has the defects of poor binding force, insufficient stability and the like.
In view of this, the present invention has been made.
Disclosure of Invention
The invention aims to provide a bacteria-rich porous denitrification filler and a preparation method thereof, which can strengthen the binding force of microorganisms, improve the stability of the denitrification filler and further solve the technical problems in the prior art.
The invention aims at realizing the following technical scheme:
the embodiment of the invention provides a bacteria-rich porous denitrification filler, which is a filler with water absorption rate of more than 30% formed by immobilizing denitrifying bacteria on a particle porous carrier with porosity of more than 90%.
The embodiment of the invention also provides a preparation method of the bacteria-rich porous denitrification filler, which is used for preparing the bacteria-rich porous denitrification filler and comprises the following steps:
step 1, preparing a particle porous carrier: adding water into tourmaline powder, kaolin, magnesia clay, diatomite, corn stalk powder, sodium silicate and sodium bicarbonate, mixing uniformly, granulating, and granulating; preheating and calcining the particles in sequence; calcining and cooling to obtain a granular porous carrier;
step 2, preparing denitrifying bacteria fermentation liquid: inoculating the cryopreserved denitrifying bacteria strain on a solid culture medium for activation, and culturing at constant temperature until single colony grows; picking the activated denitrifying bacteria single colony, and inoculating the single colony into a liquid culture medium to obtain seed liquid; inoculating the seed liquid into a liquid culture medium to obtain denitrifying bacteria fermentation liquor;
step 3, fixing denitrifying bacteria to the particle porous carrier: putting the porous carrier obtained in the step 1 into the denitrifying bacteria fermentation liquid obtained in the step 2, and adsorbing the bacteria liquid until the bacteria liquid is saturated; and (5) performing air drying treatment to obtain the bacteria-rich porous denitrification filler.
Compared with the prior art, the bacteria-rich porous denitrification filler and the preparation method thereof provided by the invention have the beneficial effects that:
the denitrifying bacteria are immobilized on the particle porous carrier with the porosity of more than 90%, so that the combination amount of the denitrifying bacteria and the porous carrier is improved, the stability of the filler is also improved, and the water absorption rate of the filler is more than 30%, so that the sufficiency of contact between sewage and the denitrifying bacteria is ensured, and the denitrification treatment effect is further improved.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the description of the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a flow chart of a preparation method of the bacteria-rich porous denitrification filler provided by the embodiment of the invention;
fig. 2 is a graph showing the comparison effect of the bacteria-rich porous denitrification filler provided by the embodiment of the invention and the quartz sand as the conventional filler.
Detailed Description
The technical scheme in the embodiment of the invention is clearly and completely described below in combination with the specific content of the invention; it will be apparent that the described embodiments are only some embodiments of the invention, but not all embodiments, which do not constitute limitations of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to fall within the scope of the invention.
The terms that may be used herein will first be described as follows:
the term "and/or" is intended to mean that either or both may be implemented, e.g., X and/or Y are intended to include both the cases of "X" or "Y" and the cases of "X and Y".
The terms "comprises," "comprising," "includes," "including," "has," "having" or other similar referents are to be construed to cover a non-exclusive inclusion. For example: including a particular feature (e.g., a starting material, component, ingredient, carrier, formulation, material, dimension, part, means, mechanism, apparatus, step, procedure, method, reaction condition, processing condition, parameter, algorithm, signal, data, product or article of manufacture, etc.), should be construed as including not only a particular feature but also other features known in the art that are not explicitly recited.
The term "consisting of … …" is meant to exclude any technical feature element not explicitly listed. If such term is used in a claim, the term will cause the claim to be closed, such that it does not include technical features other than those specifically listed, except for conventional impurities associated therewith. If the term is intended to appear in only a clause of a claim, it is intended to limit only the elements explicitly recited in that clause, and the elements recited in other clauses are not excluded from the overall claim.
The term "parts by mass" means a mass ratio relationship between a plurality of components, for example: if the X component is described as X parts by mass and the Y component is described as Y parts by mass, the mass ratio of the X component to the Y component is expressed as x:y;1 part by mass may represent any mass, for example: 1 part by mass may be expressed as 1kg or 3.1415926 kg. The sum of the mass parts of all the components is not necessarily 100 parts, and may be more than 100 parts, less than 100 parts, or 100 parts or equal. The parts, proportions and percentages described herein are by mass unless otherwise indicated.
Unless specifically stated or limited otherwise, the terms "mounted," "connected," "secured," and the like should be construed broadly to include, for example: the connecting device can be fixedly connected, detachably connected or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the terms herein above will be understood by those of ordinary skill in the art as the case may be.
When concentrations, temperatures, pressures, dimensions, or other parameters are expressed as a range of values, the range is to be understood as specifically disclosing all ranges formed from any pair of upper and lower values within the range of values, regardless of whether ranges are explicitly recited; for example, if a numerical range of "2 to 8" is recited, that numerical range should be interpreted to include the ranges of "2 to 7", "2 to 6", "5 to 7", "3 to 4 and 6 to 7", "3 to 5 and 7", "2 and 5 to 7", and the like. Unless otherwise indicated, numerical ranges recited herein include both their endpoints and all integers and fractions within the numerical range.
The terms "center," "longitudinal," "transverse," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," etc. refer to an orientation or positional relationship based on that shown in the drawings, merely for ease of description and to simplify the description, and do not explicitly or implicitly indicate that the apparatus or element in question must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present disclosure.
The bacteria-rich porous denitrification filler and the preparation method thereof provided by the invention are described in detail below. What is not described in detail in the embodiments of the present invention belongs to the prior art known to those skilled in the art. The specific conditions are not noted in the examples of the present invention and are carried out according to the conditions conventional in the art or suggested by the manufacturer. The reagents or apparatus used in the examples of the present invention were conventional products commercially available without the manufacturer's knowledge.
As shown in FIG. 1, the embodiment of the invention provides a bacteria-rich porous denitrification filler, which is a filler with water absorption rate of more than 30% formed by immobilizing denitrification bacteria on a particle porous carrier with porosity of more than 90%.
In the denitrification filler, the granular porous carrier is prepared by uniformly mixing tourmaline powder, kaolin, magnesia clay, diatomite, corn straw powder, sodium silicate and sodium bicarbonate with water to prepare granules with the diameter of 8-10 mm, and then preheating and calcining the granules in sequence. Preferably, the preheating temperature of the particles is 400-500 ℃ and the preheating time is 30min; calcining the particles at 1000-1100 ℃ for 120min.
The granule porous carrier comprises the following raw materials in percentage by mass: tourmaline powder 10%, kaolin 20%, magnesia clay 40%, diatomite 10%, corn stalk powder 10%, sodium silicate 8% and sodium bicarbonate 2%.
The corn stalk powder is obtained by crushing corn stalk and sieving with 100 mesh sieve. The corn stalk powder as biological pore forming agent can raise the porosity of porous carrier, and has low cost and easy obtaining of material.
The embodiment of the invention also provides a preparation method of the bacteria-rich porous denitrification filler, which is used for preparing the bacteria-rich porous denitrification filler and comprises the following steps:
step 1, preparing a particle porous carrier: adding water into tourmaline powder, kaolin, magnesia clay, diatomite, corn stalk powder, sodium silicate and sodium bicarbonate, mixing uniformly, granulating, and granulating; preheating and calcining the particles in sequence; calcining and cooling to obtain a granular porous carrier;
step 2, preparing denitrifying bacteria fermentation liquid: inoculating the cryopreserved denitrifying bacteria strain on a solid culture medium for activation, and culturing at constant temperature until single colony grows; picking the activated denitrifying bacteria single colony, and inoculating the single colony into a liquid culture medium to obtain seed liquid; inoculating the seed liquid into a liquid culture medium to obtain denitrifying bacteria fermentation liquor;
step 3, fixing denitrifying bacteria to the particle porous carrier: putting the porous carrier obtained in the step 1 into the denitrifying bacteria fermentation liquid obtained in the step 2, and adsorbing the bacteria liquid until the bacteria liquid is saturated; and (5) performing air drying treatment to obtain the bacteria-rich porous denitrification filler.
In the step 1 of the method, the diameter of the prepared particles is 8-10 mm, and the water absorption rate is more than 30%;
the granule porous carrier comprises the following raw materials in percentage by mass:
tourmaline powder 10%, kaolin 20%, magnesia clay 40%, diatomite 10%, corn stalk powder 10%, sodium silicate 8% and sodium bicarbonate 2%.
The corn stalk powder is obtained by crushing corn stalk and sieving with 100 mesh sieve.
In the step 1 of the method, the preheating temperature of the particles is 400-500 ℃ and the preheating time is 30min;
calcining the particles at 1000-1100 ℃ for 120min.
In the step 2 of the method, denitrifying bacteria strains are stored in a refrigerator or freezer at the temperature of minus 80 ℃ in a freezing way;
the temperature of the constant temperature culture was 30 ℃.
In the step 2 of the method, inoculating into a liquid culture medium, and culturing for 24 hours at 30 ℃ and 150r/min to obtain seed liquid;
inoculating the seed liquid into a liquid culture medium according to the volume ratio of 3%, and culturing for 24 hours at the temperature of 30 ℃ and the speed of 150r/min to obtain denitrifying bacteria fermentation liquor.
In the step 2 of the method, the solid culture medium contains 1% of tryptone, 0.5% of yeast extract powder, 1% of sodium chloride and 1.8% of agar powder according to the mass ratio; adjusting the pH value to 6.8-7.2, and sterilizing for 20min at 121 ℃;
the liquid culture medium contains 1% of tryptone, 1% of yeast extract powder, 0.5% of sodium chloride and 1% of liquid filling amount 60% according to the mass ratio, the pH value is regulated to 6.8-7.2, and then the liquid culture medium is sterilized at 121 ℃ for 20min.
In the step 3 of the method, the air drying treatment is to air-dry for 2-3 hours at 45-50 ℃.
In summary, the bacteria-rich porous denitrification filler combines the immobilized microorganism technology and the denitrification filler technology, and the denitrifying bacteria are immobilized on the porous carrier with the porosity of more than 90% through high-temperature calcination, thus the bacteria-rich porous denitrification filler belongs to the adsorption immobilized microorganism technology, and has the advantages of high porosity, large specific surface area, high biological activity, uniform and dense porous structure, capability of providing perfect habitat and attachment points for the denitrifying bacteria, and good binding force and stability. The filler is applied to the denitrification biological filter, and can be used for rapidly playing a role and has strong stability because a large amount of denitrification bacteria are fixed on the filler, so that the film forming time is saved, the denitrification rate in the reactor can be effectively improved, the treatment capacity of the reactor is enhanced, and the filler has important significance for improving the problem that the total nitrogen of a sewage treatment plant does not reach the standard.
In order to clearly show the technical scheme and the technical effects provided by the invention, the bacteria-rich porous denitrification filler and the preparation method thereof provided by the embodiment of the invention are described in detail in the following by specific embodiments.
Example 1
The embodiment of the invention provides a bacteria-rich porous denitrification filler, which is a granular filler formed by immobilizing denitrifying bacteria on a granular porous carrier, wherein the porosity of the granular filler is more than 90%, and the water absorption rate is more than 30%. The bacteria-enriched porous denitrification filler is prepared by the following steps (see figure 1):
step 1, preparing a particle porous carrier: a1. according to the mass ratio, adding water into 10% tourmaline powder, 20% kaolin, 40% magnesia clay, 10% diatomite, 10% corn stalk powder, 8% sodium silicate and 2% sodium bicarbonate, uniformly mixing, granulating, and the particle size is 8-10 mm; b1. preheating the particles at 400-500 ℃ for 30min, and calcining at 1000-1100 ℃ for 120min; c1. discharging from the furnace, cooling to room temperature to obtain the granular porous carrier;
the corn stalk powder is used as a biological pore-forming agent and is obtained by crushing corn stalk and sieving the crushed corn stalk with a 100-mesh sieve. Because the corn stalk is simple and easy to obtain and low in cost, the powder can effectively improve the porosity of the granular porous carrier and reduce the preparation cost.
Step 2, preparation of denitrifying bacteria fermentation liquid: a2. dipping a denitrifying bacterial strain which is environment-friendly and stored in a refrigerator at-80 ℃, inoculating the denitrifying bacterial strain on a solid culture medium for activation, and culturing at the constant temperature of 30 ℃ until a single colony is grown; b2. picking an activated denitrifying bacteria single colony, inoculating the single colony into a liquid culture medium, and culturing for 24 hours at 30 ℃ and 150r/min, wherein the single colony is seed liquid; c2. inoculating the seed solution into a liquid culture medium according to the volume ratio of 3%, and culturing for 24 hours at the temperature of 30 ℃ at the speed of 150r/min to obtain denitrifying bacteria fermentation liquor.
In the step 2, the solid culture medium comprises (by volume ratio): 1% of tryptone, 0.5% of yeast extract powder, 1% of sodium chloride and 1.8% of agar powder, adjusting pH value to 6.8-7.2 before digestion, and sterilizing for 20min at 121 ℃.
The liquid culture medium comprises the following components in percentage by volume: 1% of tryptone, 0.5% of yeast extract, 1% of sodium chloride, 60% of liquid loading, 6.8-7.2 of pH value before digestion, and sterilization at 121 ℃ for 20min.
Step 3, fixing denitrifying bacteria to the particle porous carrier: a3, putting the porous carrier in the step 1 into the denitrifying bacteria fermentation liquid in the step 2, and adsorbing the bacteria liquid until the bacteria liquid is saturated; b3 Air-drying at 45-50deg.C for 2-3h to obtain the final product.
The application of the prepared bacteria-rich porous denitrification filler is as follows: the bacteria-rich porous denitrification filler is filled into an upflow denitrification biological filter, the filling ratio is 50-60%, and the hydraulic retention time is 1.5-2 h.
Compared with the prior art, the invention has the following positive effects:
(1) The particle porous carrier is mainly formed by sintering natural mineral materials at high temperature, and has high mechanical strength and is not easy to damage in the practical application process. The carrier uses 100-mesh corn straw and sodium bicarbonate as pore-forming agents, has fluffy structure after calcination, has uniform and dense porous structure on the surface and inside, has porosity up to more than 90%, water absorption rate up to more than 30%, can adsorb a large amount of denitrifying bacteria and prevent bacterial loss, and effectively fixes the denitrifying bacteria, thus having high bioactivity. The carrier has high porosity and large specific surface area, so that the contact area with sewage can be increased, and the treatment capacity is improved.
(2) The porous carrier adsorbs bacteria liquid and then is subjected to air drying treatment at 45-50 ℃, so that the binding force of denitrifying bacteria and materials can be enhanced, the fixation of denitrifying bacteria is facilitated, the denitrifying bacteria cannot be damaged due to low-temperature drying at 45-50 ℃, and the high biological activity of the filler is reserved.
(3) Compared with the traditional filler, the bacteria-rich porous denitrification filler is applied to the denitrification biological filter, and can rapidly play a role and has strong stability because a large amount of denitrification bacteria are fixed on the filler, so that the film forming time is saved, the denitrification rate in the reactor can be effectively improved, and the treatment capacity of the reactor is enhanced.
(4) The invention combines the immobilized microorganism technology and the denitrification filler technology, is applied to the upflow denitrification biological filter, has a certain technical innovation, and has important significance for improving the problem that the total nitrogen of the sewage treatment plant does not reach the standard.
Example 2:
the bacterial-rich porous denitrification filler prepared by the invention is filled into a simulated reactor of a denitrification biological filter, the filling ratio is 50%, the reactor adopts an upflow water inlet mode, the water inlet is domestic sewage with total nitrogen of about 25mg/L, COD of about 100mg/L, and the hydraulic retention time is 1.5h. The total nitrogen removal rate was measured by sampling every 24 hours, and a reactor packed with quartz sand was used as a control (see fig. 2). The experimental result shows that the effluent of the reactor filled with the bacteria-rich porous denitrification filler is stable for 3 days, the total nitrogen removal rate is always kept high, the total nitrogen removal rate in the early stage of the control group is quite low, the total nitrogen removal rate gradually rises along with the operation of the reactor, and the total nitrogen removal rate is stable after 15 days of operation. Running to the 30 th day, the total nitrogen removal rate of the reactor effluent filled with the bacteria-rich porous denitrification filler is 95.96%, which is far higher than 73.76% of that of the control group. Therefore, the bacteria-rich porous denitrification filler can effectively fix denitrifying bacteria and keep higher activity, so that the film forming time is saved, the denitrification efficiency of the reactor is improved, and the treatment capacity of the reactor is enhanced.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions easily contemplated by those skilled in the art within the scope of the present invention should be included in the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope of the claims. The information disclosed in the background section herein is only for enhancement of understanding of the general background of the invention and is not to be taken as an admission or any form of suggestion that this information forms the prior art already known to those of ordinary skill in the art.
Claims (8)
1. A bacteria-rich porous denitrification filler is characterized in that the denitrification filler is a filler with water absorption rate reaching more than 30% formed by immobilizing denitrification bacteria on a particle porous carrier with porosity more than 90%;
the granular porous carrier is prepared by uniformly mixing tourmaline powder, kaolin, magnesia clay, diatomite, corn stalk powder, sodium silicate and sodium bicarbonate with water to prepare granules with the diameter of 8-10 mm, and then preheating and calcining the granules in sequence; the granule porous carrier comprises the following raw materials in percentage by mass: tourmaline powder 10%, kaolin 20%, magnesia clay 40%, diatomite 10%, corn stalk powder 10%, sodium silicate 8% and sodium bicarbonate 2%.
2. A method for preparing a bacteria-rich porous denitrification filler according to claim 1, comprising:
step 1, preparing a particle porous carrier: adding water into tourmaline powder, kaolin, magnesia clay, diatomite, corn stalk powder, sodium silicate and sodium bicarbonate, mixing uniformly, granulating, and granulating; preheating and calcining the particles in sequence; calcining and cooling to obtain a granular porous carrier; the diameter of the prepared particles is 8-10 mm, and the water absorption rate is more than 30%;
the granule porous carrier comprises the following raw materials in percentage by mass: tourmaline powder 10%, kaolin 20%, magnesia clay 40%, diatomite 10%, corn stalk powder 10%, sodium silicate 8% and sodium bicarbonate 2%;
step 2, preparing denitrifying bacteria fermentation liquid: inoculating the cryopreserved denitrifying bacteria strain on a solid culture medium for activation, and culturing at constant temperature until single colony grows; picking the activated denitrifying bacteria single colony, and inoculating the single colony into a liquid culture medium to obtain seed liquid; inoculating the seed liquid into a liquid culture medium to obtain denitrifying bacteria fermentation liquor;
step 3, fixing denitrifying bacteria to the particle porous carrier: putting the porous carrier obtained in the step 1 into the denitrifying bacteria fermentation liquid obtained in the step 2, and adsorbing the bacteria liquid until the bacteria liquid is saturated; and (5) performing air drying treatment to obtain the bacteria-rich porous denitrification filler.
3. The method for preparing the bacteria-rich porous denitrification filler according to claim 2, wherein the corn stalk powder is obtained by crushing corn stalk and sieving the crushed corn stalk with a 100-mesh sieve.
4. The method for preparing the bacteria-rich porous denitrification filler according to claim 2, wherein in the step 1, the preheating temperature of the particles is 400-500 ℃ and the preheating time is 30min;
calcining the particles at 1000-1100 ℃ for 120min.
5. The method for preparing the bacteria-rich porous denitrification filler according to claim 2, wherein in the step 2, denitrifying bacteria strains are stored in a refrigerator or freezer at-80 ℃ in a freezing way;
the temperature of the constant temperature culture was 30 ℃.
6. The method for preparing the bacteria-rich porous denitrification filler according to claim 2, wherein in the step 2, the bacteria-rich porous denitrification filler is inoculated into a liquid culture medium and cultured for 24 hours at the temperature of 30 ℃ under the condition of 150r/min to obtain seed liquid;
inoculating the seed liquid into a liquid culture medium according to the volume ratio of 3%, and culturing for 24 hours at the temperature of 30 ℃ and the speed of 150r/min to obtain denitrifying bacteria fermentation liquor.
7. The method for preparing a fungus-rich porous denitrification filler according to claim 2 or 6, wherein in the step 2, the solid medium contains 1% tryptone, 0.5% yeast extract, 1% sodium chloride and 1.8% agar powder in mass ratio; adjusting the pH value to 6.8-7.2, and sterilizing for 20min at 121 ℃;
the liquid culture medium contains 1% of tryptone, 1% of yeast extract powder, 0.5% of sodium chloride and 1% of liquid filling amount 60% according to the mass ratio, the pH value is regulated to 6.8-7.2, and then the liquid culture medium is sterilized at 121 ℃ for 20min.
8. The method for producing a bacterial-enriched porous denitrification filler according to any one of claims 2 to 6, wherein in the step 3, the air-drying treatment is air-drying at 45 to 50 ℃ for 2 to 3 hours.
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