CN114097933A - Method for preparing functional biological feed by using feed mill by-products and product thereof - Google Patents

Method for preparing functional biological feed by using feed mill by-products and product thereof Download PDF

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CN114097933A
CN114097933A CN202111421982.8A CN202111421982A CN114097933A CN 114097933 A CN114097933 A CN 114097933A CN 202111421982 A CN202111421982 A CN 202111421982A CN 114097933 A CN114097933 A CN 114097933A
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feed
enzyme
bacterial
bacterial liquid
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周相超
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Hunan Baiyi Feed Technology Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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Abstract

The invention discloses a method for preparing functional biological feed by utilizing the synergy of bacterial enzyme and biological treatment of feed mill by-products and a product thereof, which comprises the following steps: (1) activating a bacterial liquid A and a bacterial liquid B; (2) uniformly stirring and mixing the feed factory byproduct mixture, the bacterial liquid A and the complex enzyme A, and carrying out primary bacterial-enzyme synergistic biological treatment reaction; (3) adding the product obtained in the step (2) into a reaction kettle for steam explosion treatment to obtain a steam explosion product; (4) uniformly stirring and mixing the steam explosion product, the bacterial liquid A, the bacterial liquid B and the complex enzyme B, and performing secondary bacterial-enzyme synergistic biological treatment reaction; (5) and (4) drying and crushing the product obtained in the step (4) to obtain the functional biological feed. The whole process of the invention is matched with each other, the process is simple, the palatability of the functional biological feed added into the feed is good, and the feed intake of animals is high.

Description

Method for preparing functional biological feed by using feed mill by-products and product thereof
Technical Field
The invention belongs to the field of feed additive production, and particularly relates to a method for preparing functional biological feed by using feed factory byproducts and the functional biological feed prepared by the method.
Background
Many byproducts and leftovers (generally called as feed mill byproducts in the invention) are produced in the production process of feed mills, and mainly comprise: the method is characterized in that rice husks generated by husking rice before rice is aged are used, corn ash generated by air-sieving and impurity-removing treatment is carried out on the corn in a warehouse, feed raw materials generated by a dust remover in the process of producing compound feed and dust of feed products are reused, if the three feed factory byproducts are treated, 5-15% of grains are saved for a feed factory, and 500-1500 ten thousand tons of grains can be saved on the assumption that 1 hundred million tons of pig feed are produced in the whole country every year. The main components of the feed mill by-products are organic matters, mainly containing cellulose, hemicellulose, lignin, ash, starch, protein and other components, and the feed mill by-products are mainly directly used as boiler fuel to be burned or even directly discharged into the environment without being treated except for being partially used for papermaking, artificial board manufacturing, active carbon, dietary fiber, biodiesel and ethanol. The current industrial production does not make full use of this renewable resource and also pollutes the environment. How to scientifically and efficiently utilize the byproduct resources of the feed mill, realize the secondary utilization of the byproducts of the feed mill, improve the use efficiency of the byproducts of the feed mill, and have great social and economic benefits and environmental protection benefits.
Disclosure of Invention
In order to overcome the defects, the invention aims to provide a method for preparing functional biological feed by using feed mill byproducts, so that the high-efficiency utilization of the feed mill byproduct resources is realized. In order to solve the technical problems, the technical scheme provided by the invention is as follows:
a method for preparing functional biological feed by using feed mill byproducts comprises the following steps:
(1) mixing different feed mill byproducts generated in the production process of a feed mill to obtain a feed mill byproduct mixture, wherein the weight parts of the rice hulls, the corn ash impurities and the dust remover powder are respectively 30-70, 10-30 and 10-70;
(2) activating saccharomyces cerevisiae, bacillus coagulans and bacillus subtilis to obtain a bacterial liquid A, and activating lactobacillus and enterococcus faecalis to obtain a bacterial liquid B;
(3) uniformly stirring and mixing a feed factory byproduct mixture, a bacterial liquid A and a complex enzyme A, and then carrying out primary enzyme synergistic biological treatment reaction to obtain a primary enzyme synergistic biological treatment product, wherein the complex enzyme A is a mixture of cellulase, amylase, neutral protease, alkaline protease, phytase, zearalenone lactone hydrolase, glucose oxidase and catalase;
(4) adding the primary bacterial enzyme and biological treatment product into a reaction kettle for steam explosion treatment to obtain a steam explosion product;
(5) and (3) uniformly stirring and mixing the steam explosion product with the bacterial liquid A, the bacterial liquid B and the complex enzyme B, and then carrying out secondary bacterial enzyme synergistic biological treatment to obtain a secondary bacterial enzyme synergistic biological treatment product. Wherein the complex enzyme B is a mixture of xylanase, glucosyltransferase, amylase, acid protease and phytase;
(6) the secondary bacterial enzyme is cooperated with the biological treatment product to be directly used in the feed or used in the feed after fluidized bed drying.
In the preparation method, the bacterial liquid A is aerobic and facultative anaerobe, the bacterial liquid B is anaerobe, and the 2 bacterial liquids are separately preserved before production, which is favorable for the stability of strains; the compound enzyme A has the main functions of degrading fiber raw materials into substances which are easier to digest and reducing the content of mycotoxin in feed mill byproducts; the complex enzyme B has the main function of degrading the damaged macromolecular substances into micromolecular substances.
In the above production method, the content of the bacterial suspension a is preferably 1.0 × 108～1.0×1091.0 × 10 cfu/g of saccharomyces cerevisiae8～1.0×109cfu/g of Bacillus coagulans and the content of 1.0 x 108～1.0×109The bacillus subtilis cfu/g is mixed according to the mass ratio of 1:1:5 and then activated to obtain a bacterial liquid; the content of the bacterial liquid B is 1.0 multiplied by 108～1.0×109The content of lactobacillus cfu/g is 1.0 multiplied by 108～1.0×109cfu/g of bacterial liquid obtained after the enterococcus faecalis is activated. The bacillus subtilis in the bacterial liquid A is large in consumption, and can consume a large amount of oxygen in the product at the initial stage of fermentation, so that an anaerobic environment is provided for the subsequent anaerobic bacterial fermentation.
In the preparation method, preferably, in the step (3), the water content of the feed mill byproduct mixture is 30-50%.
In the preparation method, preferably, in the step (3), the feed factory byproduct mixture, the bacterial liquid a and the complex enzyme a are stirred and mixed in the following weight parts: 1000-1500 parts of feed factory by-products, 5-20 parts of bacterial liquid A and 1-2 parts of complex enzyme A.
In the above preparation method, preferably, the primary bacterial enzyme synergistic biological treatment reaction is: reacting at 35-50 ℃ until the acid soluble protein content of the reaction system is more than or equal to 5.0%. The primary bacterium enzyme and biological treatment product has mellow flavor similar to sweet wine.
In the preparation method, preferably, the steam explosion treatment is to release the pressure after the pressure is maintained at 0.4-2.0 Mpa for more than or equal to 4 min.
In the preparation method, preferably, in the step (5), the steam explosion product, the bacterial liquid A and the bacterial liquid B are added with water to adjust the water content to be 30-50%, and then 1-2 parts by weight of complex enzyme B are added to perform secondary bacterial-enzyme synergistic biological treatment. In the preparation method, preferably, the secondary bacterium enzyme synergistic biological treatment reaction is carried out at 35-50 ℃ until the pH value of the reaction system is less than or equal to 4.5 and the total acid (calculated by lactic acid) content is more than or equal to 2.0%.
In the preparation method, preferably, the temperature of the material outlet for fluidized bed drying is controlled to be 50-60 ℃. So as to ensure the activity of functional substances in the functional feed.
In the preparation method, the feed factory by-product firstly performs primary bacterium-enzyme synergistic biological treatment reaction with the bacterium liquid A and the complex enzyme A, then performs steam explosion treatment, and then performs secondary bacterium-enzyme synergistic biological treatment reaction with the bacterium liquid A, the bacterium liquid B and the complex enzyme B. The primary bacterium enzyme synergistic biological treatment reaction has the following functions: 1. the primary fermentation process can carry out alcoholization and enzymolysis, and the physical structure of cells of the material can be conveniently opened after the material is soaked and biologically treated; 2. the primary bacterial enzyme synergistic biological treatment degrades mycotoxin in byproducts of a feed factory besides degrading fiber substances, guarantees product standardization and lays a foundation for the formation of standard products through subsequent steam explosion and secondary bacterial enzyme synergistic biological treatment. The steam explosion treatment mainly comprises the steps of treating fiber raw materials in byproducts of a feed factory by using high-temperature and high-pressure steam, realizing component separation and structural change of the raw materials through an instant pressure release process, bonding cellulose and hemicellulose in the byproducts of the feed factory by lignin, improving the crystallinity of the cellulose, reducing the polymerization degree, partially degrading the hemicellulose, softening the lignin and reducing the transverse connection strength under the action of the high-temperature and high-pressure steam, and when the materials under the steam pressure release pressure suddenly, expanding water vapor in pores rapidly to generate an explosion effect, partially stripping the lignin and tearing the raw materials into fine fibers; meanwhile, the mycotoxin content can be reduced through the bacterial enzyme synergistic treatment; the mycotoxin content can be further reduced through steam explosion treatment; the steam explosion product after the primary bacterial enzyme synergistic treatment is subjected to secondary bacterial enzyme synergistic biological treatment reaction, so that the content of functional substances in the steam explosion product can be increased.
In the preparation method, when primary bacterial enzyme is subjected to synergistic biological treatment, the feed factory by-products contain cellulose and hemicellulose, xylan, cellobiose and the like can be generated after the bacterial enzyme is subjected to synergistic biological treatment, starch polysaccharide can be provided and finally degraded into reducing sugar, and the reducing sugar is one of the nutrient substances which have the functional effect on the functional biological feed, so that the primary bacterial enzyme synergistic biological treatment products mainly contain cellulose, hemicellulose, xylan, cellobiose, maltose and the like. When the secondary bacterial enzyme is used for synergistic biological treatment, xylo-oligosaccharide can be generated after enzymolysis of xylan, dietary fiber can be generated after enzymolysis of fiber, and the product contains a large amount of probiotics and enzyme, so that the main components of the secondary bacterial enzyme synergistic biological treatment product comprise xylo-oligosaccharide, dietary fiber, xylanase, probiotics and the like.
The invention also provides a functional biological feed prepared by the preparation method. The functional biological feed comprises the following components: the functional biological feed comprises, by weight, 0.5-3.0% of xylooligosaccharide, 10.0-15.0% of dietary fiber, 2.0-5.0% of reducing sugar, and 2.0-5.0% of organic acid2.0-5.0%, the weight percentage content of the small peptide is 3.5-5.0%, the weight percentage content of the amino acid is 0.5-1.0%, the weight percentage content of the vitamin is 10-100 mg/kg, the weight percentage content of the trace element is 10-100 mg/kg, and the concentration of the probiotics is 1.0 multiplied by 105～8.0×105cfu/g, the concentration of the enzyme is 1.0X 103～8.0×103u/g。
In the functional biological feed, xylo-oligosaccharide is also called xylo-oligosaccharide, is functional polysaccharide formed by combining 2-7 xylose molecules by beta-1, 4 glycosidic bonds, can selectively promote the proliferation activity of intestinal bifidobacteria, has the bifidus factor function 10-20 times that of other polymeric saccharides, and can activate or promote the immune function of body cells and body fluid, thereby being beneficial to preventing the body from being invaded and damaged by pathogenic bacteria microorganisms; dietary fiber is a polysaccharide, and is considered as a seventh type of nutrient by the nutritional community, and has quite important physiological effect on animals; the reducing sugar can improve the palatability of animal feed and regulate the intestinal health of animals; the functional biological feed is rich in a plurality of digestive enzymes such as acid protease, xylosidase, amylase, non-starch polysaccharidase and the like, and can promote the digestion and absorption of the feed in the intestinal tracts of animals; the functional biological feed disclosed by the invention is rich in organic acid and xylo-oligosaccharide, can inhibit the generation of harmful bacteria in the intestinal tract of an animal body, enables probiotics to propagate in the intestinal tract in a large quantity, and can be used for treating pseudomembranous enteritis caused by using antibiotics in a large quantity, treating constipation and chronic diarrhea, improving immunity and the like.
The functional biological feed is prepared by taking the feed mill by-products as main raw materials, so that the cost is low, the by-products of the feed mill are fully utilized, and the secondary utilization of resources is realized. In the preparation process, non-nutrient substances such as crude fiber, crude ash and the like do not need to be removed, the xylo-oligosaccharide does not need to be precipitated and separated, the dietary fiber, reducing sugar, small peptide, organic acid and the like do not need to be purified and separated, and the by-products of a feed factory can be almost completely utilized. In addition, in the preparation process, a room is closed by the synergy of the bacterial enzyme and the biological treatment, a special settling chamber is established in the drying process, alkaline water is sprayed into the room to neutralize the acidic dust and waste gas generated in the drying process, the production without waste liquid, waste gas and noise is realized, and the outward zero emission of the whole production process is realized.
The invention has the beneficial effects that:
1. when the functional biological feed is prepared, the feed factory by-products are subjected to primary bacterium-enzyme synergistic biological treatment reaction with the bacterium liquid A and the compound enzyme A, then subjected to steam explosion treatment, and subjected to secondary bacterium-enzyme synergistic biological treatment reaction with the bacterium liquid A, the bacterium liquid B and the compound enzyme B, the whole process is mutually matched and forms a system, the feed factory by-products are subjected to full bacterium-enzyme synergistic biological treatment, and the utilization rate of the feed factory by-products is high.
2. The invention prepares the full biological treatment feed, degrades the mycotoxin content, changes unsafe feed factory byproducts into safe feed and can be normally used.
3. The functional biological feed prepared by adopting the feed factory by-product with low cost comprises xylo-oligosaccharide, dietary fiber, reducing sugar, probiotics, enzyme, organic acid, small peptide, vitamin, trace elements and amino acid, and is rich in nutrition.
4. After the functional biological feed prepared by the invention is added into animal feed, the feed has good palatability and high feed intake of animals, and can supplement various nutrient substances required by the animals, improve the intestinal health of the animals and improve the immunity of the animals.
Detailed Description
In order to facilitate an understanding of the present invention, the present invention will be described more fully and in detail with reference to the preferred embodiments, but the scope of the present invention is not limited to the specific embodiments described below.
Unless otherwise defined, all terms of art used hereinafter have the same meaning as commonly understood by one of ordinary skill in the art. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the present invention.
Unless otherwise specifically stated, various raw materials, reagents, instruments, equipment and the like used in the present invention are commercially available or can be prepared by existing methods.
Example 1:
a method for preparing functional biological feed by utilizing feed factory byproduct bacterium enzyme to cooperate with biological treatment comprises the following steps:
(1) mixing different feed mill byproducts generated in the production process of a feed mill according to a certain proportion to obtain a feed mill byproduct mixture, wherein the mixing proportion of the rice hulls, the corn ash and the dust remover powder is 50:25:25, and mixing for later use;
(2) the content of 1.0 is multiplied by 1091.0 × 10 cfu/g of saccharomyces cerevisiae9cfu/g of Bacillus coagulans with a content of 1.0 × 109Adding cfu/g of bacillus subtilis into a culture medium according to the mass ratio of 1:1:5, adding the culture medium into a No. 1 strain tank, and activating to obtain a bacterial liquid A with the content of 1.0 multiplied by 109Adding cfu/g of lactobacillus into the culture medium, and then adding the lactobacillus into the No. 2 strain tank for activation to obtain a bacterium liquid B;
(3) adding the feed factory byproduct mixture, the bacterial liquid A, the complex enzyme A and the water into a mixer, stirring and mixing uniformly, adjusting the water content to 40%, and performing a bacterial-enzyme synergistic biological treatment reaction at 40 ℃ until the reaction system has special alcohol fragrance generated by fermentation, wherein the content of acid soluble protein is more than or equal to 5%. Wherein the complex enzyme A is a mixture of cellulase, amylase, neutral protease, alkaline protease, phytase, zearalenone lactone hydrolase, glucose oxidase, and catalase. The addition amount of the feed factory byproduct mixture, the bacterial liquid A and the complex enzyme A is as follows: 1 ton of feed factory byproduct mixture, 400 kg of clean water, 20 kg of bacterial liquid A and 1 kg of complex enzyme A;
(4) adding the primary bacterial enzyme synergistic biological treatment product obtained in the step (3) into a reaction kettle, maintaining the pressure at 0.8Mpa of steam pressure for more than or equal to 4min under a steam explosion platform, and releasing the pressure to obtain a steam explosion product;
(5) mixing the steam explosion product obtained in the step (4) with the bacterial liquid A and the bacterial liquid B according to 1500 kg, 20 kg and 50 kg respectively, simultaneously adding 1 kg of complex enzyme B, stirring and mixing uniformly, reacting at 40 ℃ until the pH value of a reaction system is less than or equal to 4.5, and obtaining a secondary bacterial enzyme synergistic biological treatment product with the total acid (calculated by lactic acid) content of more than or equal to 2.0 percent, wherein the complex enzyme B is a mixture of xylanase, glucotransglycosidase, amylase, acid protease and phytase;
(6) the secondary bacterium enzyme synergistic biological treatment product obtained in the step (5) can be directly used in feed; or the biological feed can be used in the feed after fluidized bed drying, and the air outlet temperature of the fluidized bed dryer is controlled at 60 ℃ for drying and then crushing to obtain the functional biological feed in the embodiment.
The functional biological feed prepared in this example comprises the following components: the food comprises, by weight, 0.5-3.0% of xylooligosaccharide, 10.0-15.0% of dietary fiber, 2.0-5.0% of reducing sugar, 2.0-5.0% of organic acid, 3.5-5.0% of small peptide, 0.5-1.0% of amino acid, 10-100 mg/kg of vitamin, 10-100 mg/kg of trace elements and 1.0 multiplied by 10 of probiotics, wherein the content of the xylooligosaccharide is 0.5-3.0%, the content of the dietary fiber is 10.0-15.0%, the content of the reducing sugar is 2.0-5.0%, the content of the organic acid is 2.0-5.0%, the content of the small peptide is 0.5-1.0%, and the content of the probiotics is 1.0 multiplied by 105～8.0×105cfu/g, enzyme concentration 1.0X 103～8.0×103u/g。
The detection method of the components comprises the following steps: the content of the xylo-oligosaccharide is detected according to the method in GB/T23747-2009 feed additive xylo-oligosaccharide; the content of the dietary fiber is detected according to the method in GB 5009.88-2014 determination of the dietary fiber in the national standard food for food safety; the content of reducing sugar is detected according to the method in GB 5009.7-2016 (determination of reducing sugar in food safety national standard food); the content of the organic acid is detected according to the method in GB/T22142-2008 'general requirements for organic acids of feed additives'; the content of the small peptide is detected according to the method in GB/T22492-2008 & ltsoybean peptide powder & gt; the content of amino acids was determined as described in GB/T18246 & 2000 determination of amino acids in feed; the content of the vitamin is detected according to the method in NY/T1029-2006 vitamin premixed feed for piglets and growing-finishing pigs; the content of the trace elements is detected according to the method in GB/T13885-2003 atomic absorption spectrometry for measuring the content of calcium, copper, iron, magnesium, manganese, potassium, sodium and zinc in animal feed; the content of the probiotics is detected according to the methods in GB 4789.35-2016 (national food safety Standard for food microbiology inspection for lactic acid bacteria), SN/T2566 (2010) Petrifilm test method for counting mould and saccharomycetes in food and GB/T26428 (2010) detection of bacillus subtilis in feeding microbial preparations); the enzyme content was determined as described in QB/T1803-1993 general test methods for Industrial enzyme preparations.
Example 2:
a method for preparing functional biological feed by utilizing feed factory byproduct bacterium enzyme to cooperate with biological treatment comprises the following steps:
(1) mixing different feed mill byproducts generated in the production process of a feed mill according to a certain ratio to obtain a feed mill byproduct mixture, wherein the mixing ratio of the rice hulls, the corn ash and the dust remover powder is 25:25:50, and mixing for later use;
(2) the content of 1.0 is multiplied by 1091.0 × 10 cfu/g of saccharomyces cerevisiae9cfu/g of Bacillus coagulans with a content of 1.0 × 109Adding cfu/g of bacillus subtilis into a culture medium according to the mass ratio of 1:1:5, adding the culture medium into a No. 1 strain tank, and activating to obtain a bacterial liquid A with the content of 1.0 multiplied by 109Adding cfu/g of lactobacillus into the culture medium, and then adding the lactobacillus into the No. 2 strain tank for activation to obtain a bacterium liquid B;
(3) adding the feed factory byproduct mixture, the bacterial liquid A, the complex enzyme A and the water into a mixer, stirring and mixing uniformly, adjusting the water content to 35%, and performing a bacterial-enzyme synergistic biological treatment reaction at 40 ℃ until the reaction system has special alcohol fragrance generated by fermentation, wherein the content of acid soluble protein is more than or equal to 5%. Wherein the complex enzyme A is a mixture of cellulase, amylase, neutral protease, alkaline protease, phytase, zearalenone lactone hydrolase, glucose oxidase, and catalase. The addition amount of the feed factory byproduct mixture, the bacterial liquid A and the complex enzyme A is as follows: 1 ton of feed factory byproduct mixture, 400 kg of clean water, 20 kg of bacterial liquid A and 1 kg of complex enzyme A;
(4) adding the primary bacterial enzyme synergistic biological treatment product obtained in the step (3) into a reaction kettle, maintaining the pressure at 0.8Mpa of steam pressure for more than or equal to 4min under a steam explosion platform, and releasing the pressure to obtain a steam explosion product;
(5) mixing the steam explosion product obtained in the step (4) with the bacterial liquid A and the bacterial liquid B according to 1500 kg, 20 kg and 50 kg respectively, simultaneously adding 1 kg of complex enzyme B, stirring and mixing uniformly, reacting at 40 ℃ until the pH value of a reaction system is less than or equal to 4.5, and obtaining a secondary bacterial enzyme synergistic biological treatment product with the total acid (calculated by lactic acid) content of more than or equal to 2.0 percent, wherein the complex enzyme B is a mixture of xylanase, glucotransglycosidase, amylase, acid protease and phytase;
(6) the secondary bacterium enzyme synergistic biological treatment product obtained in the step (5) can be directly used in feed; or the biological feed can be used in the feed after fluidized bed drying, and the air outlet temperature of the fluidized bed dryer is controlled at 60 ℃ for drying and then crushing to obtain the functional biological feed in the embodiment.
The functional biological feed prepared in this example comprises the following components: the food comprises, by weight, 0.5-3.0% of xylooligosaccharide, 10.0-15.0% of dietary fiber, 2.0-5.0% of reducing sugar, 2.0-5.0% of organic acid, 3.5-5.0% of small peptide, 0.5-1.0% of amino acid, 10-100 mg/kg of vitamin, 10-100 mg/kg of trace elements and 1.0 multiplied by 10 of probiotics, wherein the content of the xylooligosaccharide is 0.5-3.0%, the content of the dietary fiber is 10.0-15.0%, the content of the reducing sugar is 2.0-5.0%, the content of the organic acid is 2.0-5.0%, the content of the small peptide is 0.5-1.0%, and the content of the probiotics is 1.0 multiplied by 105～8.0×105cfu/g, enzyme concentration 1.0X 103～8.0×103u/g。
The above-mentioned components were detected in the same manner as in example 1.
The functional biological feeds prepared in examples 1-2 were used in the weaned pig feeding test as shown in table 1 below.
The functional biological feed prepared in example 1 and example 2 was used in the feeding test of weaned piglets as follows:
200 weaned piglets in 20 litters are selected and averagely divided into 4 groups according to the principle of similar weight, each group is 5 repeated, each weaned piglet is 10 repeated, 1 control group feed and 2 test group feeds are fed respectively, the test is started after 3 days of pre-feeding, and the formal test is 14 days. The control group was fed with Kaikoule 800S creep feed (provided by hunan Baiyi feed Co., Ltd.), test 1 group was fed with Kaikoule 800S creep feed + 5% of the product of the invention of example 1, and test 2 group was fed with Kaikoule 800S creep feed + 5% of the product of the invention of example 2, and the growth performance indexes of piglets were observed and counted during the test. The test results are shown in Table 1.
Table 1: effect of functional biological feed prepared in examples 1 and 2 on growth Performance of weaned piglets
Figure BDA0003376894880000041
Figure BDA0003376894880000051
As can be seen from table 1, the test group in which weaned piglets were fed with the functional biological feed of the present invention compared to the control group: the daily gain of the group 1 is increased by 10.02%, the daily feed intake is increased by 2.47%, the feed consumption and weight gain ratio is reduced by 6.86%, the daily gain and the daily feed intake of the weaned piglets of the group 1 are obviously superior to those of the control group, and the diarrhea rate of the piglets of the group 1 is obviously reduced compared with that of the control group; the daily gain of the test 2 group is improved by 8.51 percent, the daily feed intake is improved by 3.09 percent, the feed consumption and weight gain ratio is reduced by 5.0 percent, the daily gain and the daily feed intake of the weaned piglets of the test 2 group are both obviously superior to those of the control group, and the diarrhea rate of the piglets of the test 2 group is obviously reduced compared with that of the control group; the daily gain of the 3 groups is improved by 9.90%, the daily feed intake is improved by 5.19%, the feed consumption and weight gain ratio is reduced by 4.29%, the daily gain and the daily feed intake of the 3 groups of weaned piglets are both obviously superior to those of the control group, and the diarrhea rate of the 3 groups of piglets is obviously reduced compared with that of the control group.

Claims (10)

1. The method for preparing the functional biological feed by utilizing the feed mill byproducts is characterized by comprising the following steps of:
(1) mixing different feed mill byproducts generated in the production process of a feed mill to obtain a feed mill byproduct mixture, wherein the weight parts of the rice hulls, the corn ash impurities and the dust remover powder are respectively 10-70, 10-30 and 10-70;
(2) activating saccharomyces cerevisiae, bacillus coagulans and bacillus subtilis to obtain a bacterial liquid A, and activating lactobacillus to obtain a bacterial liquid B;
(3) uniformly stirring and mixing the feed factory byproduct mixture, the bacterial liquid A and the complex enzyme A, and then carrying out primary bacterial-enzyme synergistic biological treatment reaction to obtain a primary bacterial-enzyme synergistic biological treatment product; wherein the complex enzyme A is a mixture of cellulase, amylase, neutral protease, alkaline protease, phytase, zearalenone lactone hydrolase, glucose oxidase and catalase;
(4) adding the primary bacterial enzyme and biological treatment product into a reaction kettle for steam explosion treatment to obtain a steam explosion product;
(5) uniformly stirring and mixing the steam explosion product with the bacterial liquid A, the bacterial liquid B and the complex enzyme B, and then carrying out secondary bacterial enzyme synergistic biological treatment to obtain a secondary bacterial enzyme synergistic biological treatment product; wherein the complex enzyme B is a mixture of xylanase, glucosyltransferase, amylase, acid protease and phytase;
(6) the secondary bacterial enzyme is cooperated with the biological treatment product to be directly used in the feed or used in the feed after fluidized bed drying.
2. The method of claim 1, wherein the bacterial liquid A is contained in an amount of 1.0X 108～1.0×1091.0 × 10 cfu/g of saccharomyces cerevisiae8～1.0×109cfu/g of Bacillus coagulans and the content of 1.0 x 108～1.0×109cfu/g of bacillus subtilis is prepared according to the mass ratio of 1:1:5 and then activated to obtain a bacterial liquid, wherein the bacterial liquid B is 1.0 multiplied by 10 in content8～1.0×109Of cfu/gAnd (3) activating the lactobacillus to obtain a bacterial liquid.
3. The method for preparing functional biological feed according to claim 1, wherein the water content of the feed mill byproduct mixture is 30-50%.
4. The method for preparing functional biological feed according to claim 1, wherein in the step (3), the mixture of the bacterial liquid A, the complex enzyme A and the feed factory by-products is stirred and mixed in the following weight parts: the bacterial liquid A5-20 parts, the complex enzyme A1-2 parts and the feed mill by-product 1000-1500 parts.
5. The method for preparing functional biological feed according to claim 1 or 4, wherein the primary enzyme synergistic biological treatment reaction is carried out at 35-50 ℃ until the acid soluble protein content of the reaction system is more than or equal to 5.0%.
6. The method for preparing functional biological feed according to claim 1, wherein the steam explosion treatment is to release the pressure after maintaining the pressure at 0.4-2.0 MPa for 4min or more.
7. The method for preparing the functional biological feed according to claim 1, wherein in the step (5), the steam explosion product, the bacterial liquid A and the bacterial liquid B are adjusted to have the water content of 30-50%, and 1-2 parts by weight of the complex enzyme B is added.
8. The method for preparing functional biological feed according to claim 1 or 7, wherein the secondary bacterial enzyme synergistic biological treatment reaction is carried out at 35-50 ℃ until the pH value of the reaction system is less than or equal to 4.5, and the total acid content in terms of lactic acid is greater than or equal to 2.0%.
9. The method for preparing functional biological feed according to claim 1, wherein the outlet temperature of the fluidized bed drying material is controlled at 50-60 ℃.
10. A functional biological feed, which is characterized by comprising the following components: the functional biological feed comprises, by weight, 0.5-3.0% of xylooligosaccharide, 10.0-15.0% of dietary fiber, 2.0-5.0% of reducing sugar, 2.0-5.0% of organic acid, 3.5-5.0% of small peptide, 0.5-1.0% of amino acid, 10-100 mg/kg of vitamin, 10-100 mg/kg of trace elements and 1.0 multiplied by 10 of probiotics, wherein the concentration of the functional biological feed is 1.0-10.0% of xylooligosaccharide, the content of dietary fiber, vitamin, trace elements and probiotics, and the content of the amino acids and the reducing sugar is 2.0-5.0%, and the content of the organic acid and the small peptide is 2.0-5.0%, and the content of the probiotics is 1.0 multiplied by 105～8.0×105cfu/g, the concentration of the enzyme is 1.0X 103～8.0×103u/g。
CN202111421982.8A 2021-11-26 2021-11-26 Method for preparing functional biological feed by using feed mill by-products and product thereof Pending CN114097933A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103815119A (en) * 2013-12-31 2014-05-28 吉林中粮生化有限公司 Method for producing microbial feed by fermenting corn deep processing byproducts
CN106942470A (en) * 2017-03-22 2017-07-14 南京致润生物科技有限公司 Biological fermentation feed produced using agricultural wastes and its preparation method and application
CN107410704A (en) * 2017-08-14 2017-12-01 湖南三元农牧有限公司 The functional feedstuff additive for preparing the method for functional feedstuff additive using bagasse and being prepared
CN110613050A (en) * 2019-09-27 2019-12-27 四川铁骑力士实业有限公司 Bean curd residue fermented feed and preparation method and application thereof
CN113308449A (en) * 2021-06-24 2021-08-27 江南大学 Mutant S162P of zearalenone lactone hydrolase with improved thermal stability and application thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103815119A (en) * 2013-12-31 2014-05-28 吉林中粮生化有限公司 Method for producing microbial feed by fermenting corn deep processing byproducts
CN106942470A (en) * 2017-03-22 2017-07-14 南京致润生物科技有限公司 Biological fermentation feed produced using agricultural wastes and its preparation method and application
CN107410704A (en) * 2017-08-14 2017-12-01 湖南三元农牧有限公司 The functional feedstuff additive for preparing the method for functional feedstuff additive using bagasse and being prepared
CN110613050A (en) * 2019-09-27 2019-12-27 四川铁骑力士实业有限公司 Bean curd residue fermented feed and preparation method and application thereof
CN113308449A (en) * 2021-06-24 2021-08-27 江南大学 Mutant S162P of zearalenone lactone hydrolase with improved thermal stability and application thereof

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