CN114094988B

CN114094988B - Pulse generating circuit, equipment and method with pulse detection function

Info

Publication number: CN114094988B
Application number: CN202210056090.0A
Authority: CN
Inventors: 马剑豪; 衷兴华; 汪龙; 杨克
Original assignee: Hangzhou Vena Anke Medical Technology Co ltd
Current assignee: Hangzhou Vena Anke Medical Technology Co ltd
Priority date: 2022-01-18
Filing date: 2022-01-18
Publication date: 2022-09-09
Anticipated expiration: 2042-01-18
Also published as: CN114094988A

Abstract

The embodiment of the application provides a pulse generating circuit, equipment and a method with a pulse detection function. The pulse generating circuit with the pulse detection function comprises: a first pulse generating sub-circuit and a first driving circuit; the first driving circuit comprises a first control circuit, a first lead and at least one first gate pole driving circuit group, the first control circuit is in magnetic induction connection with each first gate pole driving circuit group through the first lead, and each first gate pole driving circuit group is correspondingly and electrically connected with a first switch module and a second switch module of one first pulse generating unit; the first control circuit causes the first pulse generating sub-circuit to output a first working pulse signal or a first detection pulse signal to the biological tissue. According to the embodiment of the application, the switch module can be protected through the first lead, and the requirement of the energy storage module of the first pulse generation sub-circuit on quick voltage recovery is met; meanwhile, the real-time curative effect in the irreversible electroporation tumor ablation process can be monitored in real time, and the ablation effect is improved.

Description

Pulse generating circuit, equipment and method with pulse detection function

Technical Field

The present application relates to the field of pulse technology, and in particular, to a pulse generation circuit, a device and a method with a pulse detection function.

Background

The irreversible electroporation technology of the high-voltage pulse electric field adopts a pulse generating circuit to output pulse signals to melt target cells of biological tissues, can kill the target cells and achieves the effect of controlling tumors. With the continuous promotion of clinical application of irreversible electroporation tumor ablation, clinicians find that examining the ablation effect by means of magnetic resonance imaging or CT scanning only 24h or more after surgery has been difficult to meet the treatment requirements of today. However, after the existing pulse generating circuit outputs a pulse signal to a biological tissue to perform ablation of target cells, the real-time curative effect in the irreversible electroporation tumor ablation process cannot be evaluated, so that the ablation effect is poor.

Moreover, most of the high-voltage pulse sources output by the pulse generating circuit are isolated from the driving circuit by using a magnetic driving transformer to provide gate driving. However, the magnetic driving transformer can only control one switch, and the synchronization performance is poor, so that the switch device is easily burnt, and the switch module is damaged.

Disclosure of Invention

The application aims at the defects of the existing mode and provides a pulse generating circuit, equipment and a method with a pulse detection function, and the technical problem that the ablation effect is poor or a switch module of the pulse generating circuit is easy to damage due to the fact that the real-time curative effect in the irreversible electroporation tumor ablation process cannot be evaluated in the prior art is solved.

In a first aspect, an embodiment of the present application provides a pulse generation circuit with a pulse detection function, including: a first pulse generation sub-circuit and a first drive circuit;

the first pulse generation sub-circuit comprises at least one first pulse generation unit which is cascaded in sequence, and the first pulse generation unit comprises a first energy storage module, a first switch module and a second switch module;

the first driving circuit comprises a first control circuit, a first lead and at least one first gate pole driving circuit group, the first control circuit is in magnetic induction connection with each first gate pole driving circuit group through the first lead, and each first gate pole driving circuit group is correspondingly and electrically connected with a first switch module and a second switch module of one first pulse generation unit;

the first control circuit is used for generating a first control signal and transmitting the first control signal to each first gate pole driving circuit group, so that each first gate pole driving circuit group correspondingly controls the connection or disconnection of the first switch module and the second switch module of each first pulse generation unit, and the first pulse generation sub-circuit outputs a first working pulse signal or a first detection pulse signal to biological tissues; the first working pulse signal is used for ablation of target cells of the biological tissue, and the first detection pulse signal is used for detecting a biological property of the biological tissue.

In one possible implementation, the first control circuit comprises a first half-bridge control circuit, a first magnetic drive signal generator and a first signal control power supply;

the first magnetic drive signal generator is electrically connected with the first half-bridge control circuit and used for outputting a first signal to the first half-bridge control circuit;

the first signal control power supply is electrically connected with the first half-bridge control circuit and is used for outputting a second signal to the first half-bridge control circuit;

the first half-bridge control circuit is used for converting the second signal into a first control signal under the control of the first signal and controlling each first gate pole driving circuit group to transmit the first control signal through a first lead, and the first control signal is a bipolar pulse signal.

In one possible implementation, the first gate driving circuit group includes a first gate modulation circuit and a second gate modulation circuit;

the first gate pole modulation circuit is correspondingly and electrically connected with the first switch module of the first pulse generation unit and controls the on/off of the first switch module;

the second gate modulation circuit is correspondingly and electrically connected with the second switch module of the first pulse generation unit and controls the on or off of the second switch module.

In one possible implementation manner, in the first pulse generating unit, a first end of the first switch module is electrically connected to a first end of the first energy storage module, a second end of the second switch module is electrically connected to a second end of the first energy storage module, and a second end of the first switch module is electrically connected to a first end of the second switch module;

the first end and the second end of the first energy storage module of the first pulse generation unit are respectively used for being electrically connected with the first end and the second end of the first power supply;

in any two adjacent first pulse generating units, the first end of the first energy storage module of the next first pulse generating unit is electrically connected with the first end of the first switch module of the previous first pulse generating unit; the second end of the first energy storage module of the next first pulse generation unit is electrically connected with the second end of the first switch module of the previous first pulse generation unit and the first end of the second switch module;

the output of the last first pulse generating unit is used for electrically connecting with the biological tissue.

In one possible implementation manner, the pulse generating circuit with a pulse detection function further includes: a second pulse generation sub-circuit and a second drive circuit;

the second pulse generation sub-circuit comprises at least one second pulse generation unit which is cascaded in sequence, and the second pulse generation unit comprises a second energy storage module, a third switch module and a fourth switch module;

the second driving circuit comprises a second control circuit, a second lead and at least one second gate driving circuit group, the second control circuit is in magnetic induction connection with each second gate driving circuit group through the second lead, and each second gate driving circuit group is correspondingly and electrically connected with a third switch module and a fourth switch module of a second pulse generating unit;

and the second control circuit is used for generating a second control signal and transmitting the second control signal to each second gate electrode driving circuit group, so that each second gate electrode driving circuit group correspondingly controls the on or off of the fourth switch module and the fourth switch module of each second pulse generation unit.

In one possible implementation manner, the first pulse generation sub-circuit and the second pulse generation sub-circuit are used for forming a first pulse generation loop with the biological tissue in a first pulse generation stage, and the first pulse generation sub-circuit outputs a first working pulse signal to the biological tissue; in a second pulse generation stage, the first pulse generation sub-circuit and the second pulse generation sub-circuit form a second pulse generation loop with the biological tissue, and the second pulse generation sub-circuit outputs a second working pulse signal to the biological tissue; the second working pulse signal is used for ablation of target cells of the biological tissue, and the first working pulse signal and the second working pulse signal are pulse signals with opposite polarities.

In one possible implementation manner, in the second pulse generating unit, a first end of a third switching module is electrically connected to a first end of the second energy storage module, a second end of a fourth switching module is electrically connected to a second end of the second energy storage module, and a second end of the third switching module is electrically connected to a first end of the fourth switching module;

the first end and the second end of the second energy storage module of the first second pulse generation unit are respectively used for being electrically connected with the first end and the second end of the second power supply;

in any two adjacent second pulse generation units, the first end of the second energy storage module of the next second pulse generation unit is electrically connected with the first end of the third switch module of the previous second pulse generation unit; the second end of the second energy storage module of the second pulse generation unit is electrically connected with the second end of the third switch module of the previous second pulse generation unit and the first end of the fourth switch module;

the output of the last second pulse generating unit is used for electrical connection with the biological tissue.

In one possible implementation manner, the first pulse generation sub-circuit further includes a first voltage-dividing resistor, a first end of the first voltage-dividing resistor is electrically connected to the output end of the last first pulse generation unit, and a second end of the first voltage-dividing resistor is used for being electrically connected to the biological tissue; and/or the presence of a gas in the gas,

the second pulse generation sub-circuit further comprises a second voltage-dividing resistor, wherein a first end of the second voltage-dividing resistor is electrically connected with the output end of the last second pulse generation unit, and a second end of the second voltage-dividing resistor is used for being electrically connected with the biological tissue.

In one possible implementation manner, the first pulse generation sub-circuit and the second pulse generation sub-circuit are used for forming a third pulse generation loop with the biological tissue in a third pulse generation stage and outputting a first detection pulse signal to the biological tissue; in the third pulse generation circuit, the first energy storage modules of the first pulse generation sub-circuits are all in a parallel charging state, the output voltage of the output end of the last first pulse generation unit is the voltage of the first power supply, the first divider resistor is connected to the third pulse generation circuit, and the biological tissue is grounded through the second pulse generation sub-circuit.

In one possible implementation manner, the first pulse generation sub-circuit and the second pulse generation sub-circuit are used for forming a fourth pulse generation loop with the biological tissue in a fourth pulse generation stage and outputting a second detection pulse signal to the biological tissue; in a fourth pulse generation loop, second energy storage modules of second pulse generation sub-circuits are all in a parallel charging state, the output voltage of the output end of the last second pulse generation unit is the voltage of a second power supply, a second divider resistor is connected into the fourth pulse generation loop, and biological tissues are grounded through the first pulse generation sub-circuit; the second detection pulse signal and the first detection pulse signal are pulse signals with opposite polarities; the second detection pulse signal is used to detect a biological property of the biological tissue.

In one possible implementation, the second control circuit includes a second half-bridge control circuit, a second magnetic drive signal generator, and a second signal control power supply;

the second magnetic drive signal generator is electrically connected with the second half-bridge control circuit and used for outputting a third signal to the second half-bridge control circuit;

the second signal control power supply is electrically connected with the second half-bridge control circuit and used for outputting a fourth signal to the second half-bridge control circuit;

and the second half-bridge control circuit is used for converting the fourth signal into a second control signal under the control of the third signal and controlling each second gate drive circuit group to transmit the second control signal through a second lead, wherein the second control signal is a bipolar pulse signal.

In a second aspect, embodiments of the present application provide a pulse generation device, which includes a pulse generation circuit with a pulse detection function as in the first aspect.

In one possible implementation, the pulse generating apparatus further includes: a control unit;

a control unit for determining a biological property of the biological tissue from the first detection pulse signal, and adjusting a pulse parameter of the first detection pulse signal and/or the first working pulse signal according to the biological property of the biological tissue, wherein the pulse parameter comprises at least one of: pulse width, frequency, amplitude.

In a third aspect, an embodiment of the present application further provides a pulse generating method, which is applied to the pulse generating circuit with a pulse detection function in the first aspect, and includes:

the first control circuit generates a first control signal and transmits the first control signal to each first gate pole driving circuit group;

each first gate pole driving circuit group correspondingly controls the connection or disconnection of the first switch module and the second switch module of each first pulse generation unit according to the first control signal, so that the first pulse generation sub-circuit outputs a first working pulse signal or a first detection pulse signal to the biological tissue; the first working pulse signal is used for ablation of target cells of the biological tissue, and the first detection pulse signal is used for detecting a biological property of the biological tissue.

In one possible implementation, the first pulse generating sub-circuit outputs a first working pulse signal to the biological tissue, including:

in a first pulse generation stage, a first pulse generation sub-circuit and a second pulse generation sub-circuit form a first pulse generation loop with biological tissues, and the first pulse generation sub-circuit outputs a first working pulse signal to the biological tissues; in the first pulse generation loop, the first energy storage modules of the first pulse generation sub-circuit are sequentially connected in series, the second energy storage modules of the second pulse generation sub-circuit are not connected, and the biological tissue is grounded through the second pulse generation sub-circuit.

In one possible implementation, the first pulse generating sub-circuit outputs a first detection pulse signal to the biological tissue, including:

in a third pulse generation stage, the first pulse generation sub-circuit, the first divider resistor, the second pulse generation sub-circuit and the biological tissue form a third pulse generation loop, and a first detection pulse signal is output to the biological tissue; in the third pulse generation circuit, the first energy storage modules of the first pulse generation sub-circuits are all in a parallel charging state, the output voltage of the output end of the last first pulse generation unit is the voltage of the first power supply, the first divider resistor is connected to the third pulse generation circuit, and the biological tissue is grounded through the second pulse generation sub-circuit.

In one possible implementation, the pulse generation method further comprises at least one of:

in a second pulse generation stage, the first pulse generation sub-circuit and the second pulse generation sub-circuit form a second pulse generation loop with the biological tissue, and the second pulse generation sub-circuit outputs a second working pulse signal to the biological tissue; in the second pulse generation loop, the second energy storage modules of the second pulse generation sub-circuit are sequentially connected in series, the first energy storage modules of the first pulse generation sub-circuit are not connected, and the biological tissue is grounded through the first pulse generation sub-circuit;

in a fourth pulse generation stage, the first pulse generation sub-circuit, the second pulse generation sub-circuit and the biological tissue form a fourth pulse generation loop, and a second detection pulse signal is output to the biological tissue; in a fourth pulse generation loop, second energy storage modules of second pulse generation sub-circuits are all in a parallel charging state, the output voltage of the output end of the last second pulse generation unit is the voltage of a second power supply, a second voltage dividing resistor is connected to the fourth pulse generation loop, and biological tissues are grounded through the first pulse generation sub-circuit; the second detection pulse signal and the first detection pulse signal are pulse signals with opposite polarities; the second detection pulse signal is used to detect a biological characteristic of the biological tissue.

The technical scheme provided by the embodiment of the application brings beneficial technical effects that:

each first gate driving circuit group of the pulse generating circuit is electrically connected with the first switch module and the second switch module of one first pulse generating unit correspondingly, the first control circuit generates a first control signal and transmits the first control signal to each first gate driving circuit group through a first lead by utilizing a magnetic induction law, and therefore each first gate driving circuit group can correspondingly control the connection or disconnection of the first switch module and the second switch module of each first pulse generating unit. The first drive circuit of this application embodiment only needs to control first pulse generating unit's first switch module and second switch module simultaneously through a high-voltage conductor, the withstand voltage of utilizing high-voltage conductor can realize high voltage amplitude output, utilize the second switch module to realize charging energy storage capacitor fast as the tail-end switch, do not need complicated and expensive optic fibre control circuit, thereby simplify magnetic drive's isolating circuit structure among the multistage pulse generating circuit greatly, pulse generating circuit's output voltage and output power have been improved, and can realize that magnetic drive transformer control switch switches on synchronous, thereby can protect switch module, satisfy the quick recovery voltage demand of the energy storage module of first pulse generating sub-circuit.

Moreover, the first pulse generation sub-circuit of the embodiment of the present application may output a first working pulse signal or a first detection pulse signal to the biological tissue, where the first working pulse signal is used to ablate target cells of the biological tissue and implement irreversible electroporation of cell membranes, and the first detection pulse signal is used to detect biological characteristics of the biological tissue, so as to monitor real-time curative effect during the irreversible electroporation tumor ablation process in real time, determine an optimal treatment stop time point, and improve the ablation effect.

Additional aspects and advantages of the present application will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the present application.

Drawings

The foregoing and/or additional aspects and advantages of the present application will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:

fig. 1 is a schematic structural diagram of a pulse generating circuit with a pulse detection function according to an embodiment of the present disclosure;

fig. 2 is a schematic structural diagram of another pulse generating circuit with a pulse detection function according to an embodiment of the present disclosure;

fig. 3 is a schematic structural diagram of another pulse generating circuit with a pulse detection function according to an embodiment of the present disclosure;

fig. 4 is a schematic structural diagram of an electrical connection between a pulse generating circuit with a pulse detection function and a load according to an embodiment of the present disclosure;

fig. 5 is a schematic circuit diagram of a first driving circuit according to an embodiment of the present disclosure;

fig. 6 is a schematic structural diagram of an electrical connection between a pulse generating circuit with a pulse detection function and a load according to another embodiment of the present disclosure;

FIG. 7 is a schematic diagram of the pulse generating circuit with pulse detection function of FIG. 6 electrically connected to a load in a charging stage;

FIG. 8 is a schematic diagram of the pulse generating circuit with pulse detection function of FIG. 6 electrically connected to a load at a first pulse generation stage;

FIG. 9 is a schematic diagram of the pulse generating circuit with pulse detection function of FIG. 6 electrically connected to a load at a second pulse generation stage;

FIG. 10 is a schematic diagram of the pulse generating circuit with pulse detection function of FIG. 6 electrically connected to a load at a third pulse generation stage;

FIG. 11 is a schematic diagram of the pulse generating circuit with pulse detection function of FIG. 6 electrically connected to a load at a fourth pulse generation stage;

FIG. 12 is a waveform diagram of a bipolar high voltage pulse signal according to an embodiment of the present application;

FIG. 13 is a waveform diagram of a bipolar high voltage pulse signal and a low voltage measurement signal according to an embodiment of the present application;

fig. 14 is a waveform diagram of another bipolar high voltage pulse signal and a low voltage measurement signal according to an embodiment of the present application.

Reference numerals are as follows:

110-a first power supply, 120-a first pulse generating sub-circuit, 121-a first pulse generating unit, 122-a first energy storage module, 123-a first switching module, 124-a second switching module;

130-a first driving circuit, 136-a first control circuit, 131-a first gate driving circuit group, 132-a first magnetic driving signal generator, 133-a first lead, 134-a first signal control power supply, 135-a first half-bridge control circuit, 1311-a first gate modulation circuit, 1312-a second gate modulation circuit;

140-a second power supply, 150-a second pulse generating sub-circuit, 151-a second pulse generating unit, 152-a second energy storage module, 153-a third switching module, 154-a fourth switching module;

160-a second drive circuit, 166-a second control circuit, 161-a second gate drive circuit group, 162-a second magnetic drive signal generator, 163-a second lead, 164-a second signal control power supply, 165-a second half-bridge control circuit;

170-load.

Detailed Description

Reference will now be made in detail to the present application, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar parts or parts having the same or similar functions throughout. In addition, if a detailed description of the known art is not necessary for illustrating the features of the present application, it is omitted. The embodiments described below with reference to the accompanying drawings are exemplary only for explaining the present application and are not construed as limiting the present application.

It will be understood by those within the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the prior art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

As used herein, the singular forms "a", "an", "the" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms "comprises" and/or "comprising," when used in this specification, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof. It will be understood that when an element is referred to as being "connected" or "coupled" to another element, it can be directly connected or coupled to the other element or intervening elements may also be present. Further, "connected" or "coupled" as used herein may include wirelessly connected or wirelessly coupled. As used herein, the term "and/or" includes all or any element and all combinations of one or more of the associated listed items.

The inventor of the application researches and discovers that the high-voltage pulse electric field irreversible electroporation technology is characterized in that an electrode needle is inserted into a diseased part of a patient, a plurality of nanoscale irreversible pore channels are formed on the surface of a cell membrane by releasing high-voltage electric pulses, the cell homeostasis is destroyed, the apoptosis is promoted, cell fragments after the apoptosis can be phagocytized by phagocytes in vivo, and meanwhile, the immune reaction of an organism occurs, so that the effect of controlling the tumor is achieved. The non-heat-production ablation technology has the advantages that the ablation area is clear in boundary, important tissue structures of nerves, great vessels, ureters, bronchus, large bile ducts, gastrointestinal walls and the like of an ablated area can be reserved, the ablation is not affected by heat or cold elimination of blood flow, the ablation time is short, and the like. The technology makes up the technical defects of radio frequency, microwave and cryoablation.

However, the high-voltage pulse source is a core link of the irreversible electroporation tumor ablation technology, and at present, most of the high-voltage pulse sources form a circuit based on Marx high voltage of a solid-state switch. The Marx high-voltage forming circuit is usually isolated by using a magnetic driving transformer to provide grid drive, but the magnetic driving transformer can only control one path of switch, and has poor synchronization performance, and when the Marx high-voltage forming circuit is applied to a switch series circuit, the switch is not conducted synchronously, so that the switch is burnt; particularly, when the circuit is applied to a Marx type circuit with a switch circuit, the circuit design is complex and the requirement of the Marx energy storage capacitor on quick voltage recovery cannot be met. However, if the Marx energy storage capacitor cannot recover the voltage quickly, the real-time measurement of the tissue impedance by the low-voltage pulse signal is influenced.

The application provides a pulse generating circuit with a pulse detection function, equipment and a method, which aim to solve the technical problems in the prior art.

The following describes the technical solutions of the present application and how to solve the above technical problems with specific embodiments.

An embodiment of the present application provides a pulse generating circuit with a pulse detection function, as shown in fig. 1, including: a first pulse generating sub-circuit 120 and a first driving circuit 130.

The first pulse generating sub-circuit 120 includes at least one first pulse generating unit 121 cascaded in sequence, and the first pulse generating unit 121 includes a first energy storage module 122, a first switching module 123 and a second switching module 124.

The first driving circuit 130 includes a first control circuit 136, a first conducting wire 133 and at least one first gate driving circuit group 131, the first control circuit 136 is magnetically induced and connected to each first gate driving circuit group 131 through the first conducting wire 133, and each first gate driving circuit group 131 is electrically connected to the first switching module 123 and the second switching module 124 of one first pulse generating unit 121.

A first control circuit 136, configured to generate a first control signal and transmit the first control signal to each first gate driving circuit group 131, so that each first gate driving circuit group 131 correspondingly controls the on/off of the first switch module 123 and the second switch module 124 of each first pulse generating unit 121, and the first pulse generating sub-circuit 120 outputs a first working pulse signal or a first detection pulse signal to the biological tissue; the first working pulse signal is used for ablation of target cells of the biological tissue, and the first detection pulse signal is used for detecting a biological property of the biological tissue.

The first driving circuit 130 of the embodiment of the present application can simultaneously control the first switch module 123 and the second switch module 124 of the first pulse generating unit 121 only by one high voltage conducting wire, high voltage amplitude output can be realized by using the withstand voltage of the high voltage conducting wire, the second switch module 124 is used as a tail switch to realize fast charging of the energy storage capacitor, a complex and expensive optical fiber control circuit is not needed, thereby greatly simplifying the isolation circuit structure of magnetic driving in the multi-stage pulse generating circuit, the output voltage and the output power of the pulse generating circuit are improved, and the control switch of the magnetic driving transformer can be turned on synchronously, thereby protecting the switch modules, and satisfying the requirement of fast recovery voltage of the energy storage module of the first pulse generating sub-circuit 120.

Moreover, the first pulse generator 120 of the embodiment of the present application may output a first working pulse signal or a first detection pulse signal to the biological tissue, where the first working pulse signal is used to ablate target cells of the biological tissue and implement irreversible electroporation of cell membranes, and the first detection pulse signal is used to detect biological characteristics of the biological tissue, so as to monitor real-time curative effect during the irreversible electroporation tumor ablation process in real time, determine an optimal treatment stop time point, and improve the ablation effect.

Alternatively, the first control circuit 136 transmits the first control signal to each of the first gate driving circuit groups 131 through the first conductive line 133 using the law of magnetic induction.

Optionally, the first detection pulse signal is used to detect a biological property of the biological tissue, the biological property being a characteristic of an effect of the first working pulse signal on performing ablation of target cells of the biological tissue, the biological property including a load impedance spectrum.

Optionally, the first working pulse signal is a high-voltage pulse signal, and the first detection pulse signal is a low-voltage measurement signal.

The pulse generating circuit with the pulse detection function can output a first detection pulse signal after outputting a first working pulse signal, the first detection pulse signal is used for fusion impedance spectrum real-time measurement based on magnetic driving, and the ablation effect can be detected in real time after target cells are ablated.

In some embodiments, referring to fig. 1, the first control circuit 136 includes a first half-bridge control circuit 135, a first magnetic drive signal generator 132, and a first signal control power supply 134.

The first magnetic driving signal generator 132 is electrically connected to the first half-bridge control circuit 135, and the first magnetic driving signal generator 132 is configured to output a first signal to the first half-bridge control circuit 135.

The first signal control power supply 134 is electrically connected to the first half-bridge control circuit 135, and the first signal control power supply 134 is configured to output the second signal to the first half-bridge control circuit 135.

The first half-bridge control circuit 135 is used for converting the second signal into a first control signal under the control of the first signal, and controlling each of the first gate driving circuit sets 131 to transmit the first control signal through the first conducting wire 133, wherein the first control signal is a bipolar pulse signal.

Optionally, referring to fig. 1, the first pulse generator sub-circuit 120 includes a first power source 110, the first power source 110 is a high voltage dc power source, and the first pulse generator sub-circuit 120 is a Marx main circuit. The first driving circuit 130 is a magnetic driving loop circuit, the first magnetic driving signal generator 132 is connected in parallel with the first power source 110, the first conducting wire 133 is a high-voltage conducting wire, the first conducting wire 133 is connected with the first magnetic driving signal generator 132, the first magnetic driving signal generator 132 and the first gate driving circuit group 131 are electrically connected through the first conducting wire 133 by using the law of magnetic induction, and the specific number of the first gate driving circuit group 131 in the first driving circuit 130 is set according to the number of the first pulse generating units 121 of the first pulse generating sub-circuit 120, and is the same. The first gate driving circuit group 131 is connected to the first pulse generating units 121 of each stage of the first pulse generating sub-circuit 120, respectively. The first pulse generating unit 121 may be connected in parallel with the first power source 110.

Optionally, the first signal control power source 134 is connected in parallel to the first half-bridge control circuit 135, the first magnetic driving signal generator 132 is connected to a signal input terminal of the first half-bridge control circuit 135, and an output terminal of the first half-bridge control circuit 135 and the first gate driving circuit group 131 transmit the first control signal through the first conducting wire 133 by using magnetic induction law.

Optionally, as shown in fig. 4, the first switch module 123 includes a main switch, and the second switch module 124 includes a tail switch, and the main switch and the tail switch are controlled to be turned on or off by a pulse signal output by the first driving circuit 130, so as to implement a pulse signal output to the pulse generating circuit as a whole. Optionally, both the main switch and the tail switch cannot be turned on simultaneously.

Optionally, the pulse generation circuit with the pulse detection function according to the embodiment of the present application may be provided with two sets of pulse generation sub-circuits and driving circuits to implement a bipolar pulse signal. Referring to fig. 2, the pulse generation circuit having a pulse detection function includes a first pulse generation sub-circuit 120, a first driving circuit 130, a second pulse generation sub-circuit 150, and a second driving circuit 160. The first pulse generation sub-circuit 120 and the second pulse generation sub-circuit 150 are both Marx main circuits, which correspond to a positive Marx main circuit and a negative Marx main circuit, respectively, and the first driving circuit 130 and the second driving circuit 160 are both magnetic driving units.

The pulse generating circuit with the pulse detection function can simultaneously control one main switch and one tail switch of the Marx main circuit by only using one high-voltage wire, so that a complex and expensive traditional control loop is avoided, the cost and the system complexity of the whole pulse generating circuit are greatly low, and the reliability of the pulse generating circuit is improved. The positive and negative Marx main circuits generate pulses and apply the pulses to a load 170 through electrodes, as shown in fig. 2, the load 170 representing biological tissue.

In some embodiments, referring to fig. 2, the pulse generation circuit with pulse detection function further includes: a second pulse generating sub-circuit 150 and a second driving circuit 160. The structures of the second pulse generation sub-circuit 150 and the second driving circuit 160 correspond to those of the first pulse generation sub-circuit 120 and the first driving circuit 130, the circuit principles are the same, and the beneficial technical effects that can be achieved are the same, which are not described herein again.

Optionally, the second pulse generating sub-circuit 150 includes at least one second pulse generating unit 151 cascaded in sequence, and the second pulse generating unit 151 includes a second energy storage module 152, a third switching module 153, and a fourth switching module 154.

The second driving circuit 160 includes a second control circuit 166, a second conducting wire 163 and at least one second gate driving circuit group 161, the second control circuit 166 is connected with each second gate driving circuit group 161 through the second conducting wire 163 in a magnetic induction manner, and each second gate driving circuit group 161 is electrically connected with the third switching module 153 and the fourth switching module 154 of one second pulse generating unit 151 correspondingly.

And a second control circuit 166, configured to generate a second control signal and transmit the second control signal to each second gate driving circuit group 161, so that each second gate driving circuit group 161 correspondingly controls on/off of the fourth switch module 154 and the fourth switch module 154 of each second pulse generating unit 151.

Alternatively, the second control circuit 166 transmits the second control signal to the second gate driving circuit group 161 through the second conductive line 163 using the law of magnetic induction.

In some embodiments, referring to fig. 3, the second control circuit 166 includes a second half-bridge control circuit 165, a second magnetic drive signal generator 162, and a second signal control power supply 164.

The second magnetic driving signal generator 162 is electrically connected to the second half-bridge control circuit 165, and the second magnetic driving signal generator 162 is configured to output a third signal to the second half-bridge control circuit 165.

The second signal-controlled power supply 164 is electrically connected to the second half-bridge control circuit 165, and the second signal-controlled power supply 164 is configured to output a fourth signal to the second half-bridge control circuit 165.

The second half-bridge control circuit 165 is configured to convert the fourth signal into a second control signal under the control of the third signal, and control each second gate driving circuit set 161 to transmit the second control signal through a second conducting line 163, where the second control signal is a bipolar pulse signal.

Optionally, referring to fig. 3, the second pulse generation sub-circuit 150 includes a second power supply 140, the second power supply 140 is a high voltage dc power supply, and the second pulse generation sub-circuit 150 is a Marx main circuit. The second driving circuit 160 is a magnetic driving circuit, the second magnetic driving signal generator 162 is connected in parallel with the second power source 140, the second conducting wire 163 is a high-voltage conducting wire, the second conducting wire 163 is connected with the second magnetic driving signal generator 162, the second magnetic driving signal generator 162 and the second gate driving circuit set 161 are electrically connected through the second conducting wire 163 by using the law of magnetic induction, and the specific number of the second gate driving circuit set 161 in the second driving circuit 160 is set according to the number of the second pulse generating units 151 of the second pulse generating sub-circuit 150, and the number is the same. The second gate driving circuit group 161 is connected to the second pulse generating units 151 of each stage of the second pulse generating sub-circuit 150, respectively. The second pulse generating sub-circuit 150 may be connected in parallel with the second power source 140.

Optionally, the second signal control power source 164 is connected in parallel with the second half-bridge control circuit 165, the second magnetic driving signal generator 162 is connected to a signal input terminal of the second half-bridge control circuit 165, and an output terminal of the second half-bridge control circuit 165 and the second gate driving circuit group 161 transmit the second control signal by using the magnetic induction law through the second conducting wire 163.

Alternatively, as shown in fig. 4, the third switch module 153 includes a main switch, and the fourth switch module 154 includes a tail switch, and the main switch and the tail switch are controlled to be turned on or off by the pulse signal output by the second driving circuit 160, so as to implement the pulse signal output to the pulse generating circuit as a whole. Optionally, both the main switch and the tail switch cannot be turned on simultaneously. In fig. 4, neither the first energy storage module 122 nor the second energy storage module 152 is shown.

Optionally, the first end and the second end of the main switch are respectively used as the first end and the second end of the corresponding switch module 154, and the first end and the second end of the tail switch are respectively used as the first end and the second end of the corresponding switch module 154. The control ends of the main switch and the tail switch are respectively and correspondingly electrically connected with the first gate drive circuit group 131 or the second gate drive circuit group 161.

Alternatively, the first power source 110 and the second power source 140 may be external power sources, and the power voltages of the first power source 110 and the second power source 140 may be the same or different.

Alternatively, as shown in fig. 4 and 5, the first gate driving circuit group 131 includes a first output terminal and a second output terminal, which are sequentially connected to the main switch and the tail switch of the first pulse generating unit 121.

Alternatively, each first gate driving circuit group 131 is provided with two output interfaces, the pulse signals output by the two output interfaces are opposite signals, and the two output interfaces are used as a group of interfaces, and the group of interfaces is connected with the main switch and the tail switch in each stage of the first pulse generating unit 121. The first magnetic driving signal generator 132 is a signal source capable of outputting a square wave, the square wave output by the first magnetic driving signal generator is input to the first half-bridge control circuit 135, the first half-bridge control circuit 135 converts the pulse output by the first signal control power source 134 into a bipolar square wave pulse under the control of the square wave, and the bipolar square wave pulse controls the first gate driving circuit group 131 to operate after passing through the first conducting wire 133.

Optionally, a dual-output equivalent circuit of the transformer is formed between the first conducting wire 133 and the first gate driving circuit group 131, and the equivalent circuit is implemented by using the law of magnetic induction to inductively output two opposite pulses from the two square waves output by the first magnetic driving signal generator 132, and input the two opposite pulses into the first gate driving circuit group 131 as the driving signal of the first gate driving circuit group 131.

In some embodiments, referring to fig. 5, a structure of the first driving circuit 130 is taken as an example to describe a transmission process of the control signal. The first gate drive circuit group 131 includes a first gate modulation circuit 1311 and a second gate modulation circuit 1312.

The first gate modulation circuit 1311 is electrically connected to the first switch module 123 of the first pulse generating unit 121, and controls the first switch module 123 to be turned on or off.

The second gate modulation circuit 1312 is electrically connected to the second switch module 124 of the first pulse generation unit 121, and controls on/off of the second switch module 124.

Optionally, as shown in fig. 4 and 5, the first gate modulation circuit 1311 controls the main switch of the first switch module 123 to be turned on and off, and the second gate modulation circuit 1312 controls the tail switch of the second switch module 124 to be turned on and off.

Optionally, the output terminal of the first gate modulation circuit 1311 is electrically connected to the control terminal of the main switch, and the output terminal of the second gate modulation circuit 1312 is electrically connected to the control terminal of the tail switch.

Alternatively, as shown in fig. 3 to fig. 5, the isolation circuit is implemented by using the first driving circuit 130, the first driving circuit 130 is determined by using a magnetic driving loop circuit, and the first magnetic driving signal generator 132 outputs a voltage pulse signal generated by the first signal control power source 134 through the first conducting wire 133 to implement pulse output of the first gate driving circuit group 131, so as to implement control of the main switch and the tail switch of the first pulse generating unit 121. Compared with the existing power supply isolation circuit, the magnetic drive isolation circuit has the advantages that the result is simpler, the isolation effect is good, the control is simple, the charging of the first energy storage module 122 is rapidly realized by utilizing the tail switch, the loss of the first energy storage module 122 in the discharging process is compensated, and the output voltage and the output power of the pulse generation circuit are improved.

Alternatively, fig. 5 shows a specific circuit schematic diagram of the first gate drive circuit group 131 and a PCB board, the diagram selects the gate drive circuit group to be provided with two outputs, and the first gate drive circuit group 131 includes a first gate modulation circuit 1311 and a second gate modulation circuit 1312.

Optionally, referring to fig. 5, the first gate modulation circuit includes a first input terminal, a first MOSFET (Q1-1), a second MOSFET (Q1-2), a first driving resistor (R1-1), a second driving resistor (R1-2), a first gate capacitor Cg1, a first transient suppression diode Z1, a first voltage dividing resistor Rg1, and a first output terminal; one pin of the first input is connected with the S pole of the first MOSFET (Q1-1), and the other pin of the first input is connected with the S pole of the second MOSFET (Q1-2); the G pole of the first MOSFET (Q1-1) is connected with the S pole of the second MOSFET (Q1-2) through a first driving resistor (R1-1); the G pole of the second MOSFET (Q1-2) is connected with the D pole of the second MOSFET (Q1-2) through a second driving resistor (R1-2); the first gate capacitor Cg1 is connected in series between the D pole of the first MOSFET (Q1-1) and the D pole of the second MOSFET (Q1-2); one pin of the first output end is connected with one end of a first transient suppression diode Z1, and is connected with the D pole of a second MOSFET (Q1-2) through a first divider resistor Rg 1; the other pin of the first output terminal is connected to the other terminal of the first transient suppression diode Z1, and is also connected to the D-pole of the first MOSFET (Q1-1).

Optionally, the second gate modulation circuit comprises a second input terminal, a third MOSFET (Q2-1), a fourth MOSFET (Q2-2), a third driving resistor (R2-1), a fourth driving resistor (R2-2), a second gate capacitance Cg2, a second transient suppression diode Z2, a second voltage division resistor Rg2 and a second output terminal; one pin of the second input terminal is connected with the S pole of the third MOSFET (Q2-1), and the other pin of the second input terminal is connected with the S pole of the fourth MOSFET (Q2-2); the G pole of the third MOSFET (Q2-1) is connected with the S pole of the fourth MOSFET (Q2-2) through a third driving resistor (R2-1); the G pole of the fourth MOSFET (Q2-2) is connected with the D pole of the fourth MOSFET (Q2-2) through a fourth driving resistor (R2-2); the second gate capacitance Cg2 is connected in series between the D pole of the third MOSFET (Q2-1) and the D pole of the fourth MOSFET (Q2-2); one pin of the second output end is connected with one end of a second transient suppression diode Z2, and is simultaneously connected with the D pole of a fourth MOSFET (Q2-2) through a second divider resistor Rg 2; the other pin of the second output terminal is connected with the other end of the second transient suppression diode Z2 and is also connected with the D pole of a third MOSFET (Q2-1).

Optionally, when the front end of the magnetic loop receives a bipolar square wave output by the first half-bridge control circuit 135, two gate signals with opposite polarities are respectively formed on the two gate capacitors Cg through the first gate drive circuit group 131, the amplitude of the gate signal is determined by the amplitude of the bipolar square wave, the pulse width of the gate signal is determined by the positive and negative square wave intervals of the bipolar square wave, the frequency of the gate signal is determined by the frequency of the bipolar square wave, and the two gate signals are connected to the control ends of the main switch and the tail switch, so that the main switch and the tail switch are reliably and stably controlled to be turned on and off simultaneously.

Alternatively, the second gate driving circuit group 161 includes a third gate modulation circuit and a fourth gate modulation circuit. The third gate modulation circuit is electrically connected to the third switch module 153 of the second pulse generating unit 151, and controls the third switch module 153 to be turned on or off. The fourth gate modulation circuit is electrically connected to the fourth switching module 154 of the second pulse generating unit 151, and controls the fourth switching module 154 to be turned on or off. Referring to fig. 5, the structure of the second driving circuit 160 corresponds to the structure of the first driving circuit 130, and the principle of the transmission process of the control signal is the same, which is not described herein again.

Alternatively, referring to fig. 5, the first half-bridge control circuit 135 is formed by connecting a switching combination circuit (Q131 and Q132 in the figure and a capacitance combination circuit (C131 and C132 in the figure) in parallel, the first half-bridge control circuit 135 is connected in parallel with the first signal control power source 134, and both ends of the first wire 133 are connected with the switching combination circuit and the capacitance combination circuit in turn, the switching combination circuit includes a MOS transistor Q131, a MOS transistor Q132, the MOS transistor Q131 and the capacitor C132 are connected, the S pole of the MOS transistor Q131 is connected with the D pole of the MOS transistor Q132, the D pole of the MOS transistor Q131 is connected with one end of a capacitor combination circuit, the S pole of the MOS transistor Q132 is connected with the other end of the capacitor combination circuit, the capacitor combination circuit is formed by connecting two capacitors in series, one end of a first lead 133 is connected with the common connecting end of the two capacitors, the other end of the first lead 133 is connected with the D pole of the MOS transistor Q132, and the G pole of the MOS transistor Q131 and the G pole of the MOS transistor Q132 are connected with a control signal.

In some embodiments, the first pulse generating sub-circuit 120 and the second pulse generating sub-circuit 150 are configured to, in a first pulse generating phase, form a first pulse generating loop with the biological tissue by the first pulse generating sub-circuit 120 and the second pulse generating sub-circuit 150, and the first pulse generating sub-circuit 120 outputs a first working pulse signal to the biological tissue; in the second pulse generation stage, the first pulse generation sub-circuit 120 and the second pulse generation sub-circuit 150 form a second pulse generation loop with the biological tissue, and the second pulse generation sub-circuit 150 outputs a second working pulse signal to the biological tissue; the second working pulse signal is used for ablation of target cells of the biological tissue, and the first working pulse signal and the second working pulse signal are pulse signals with opposite polarities.

In some embodiments, as shown in fig. 4 and fig. 6, in the first pulse generating unit 121, a first end of the first switching module 123 is electrically connected to a first end of the first energy storage module 122, a second end of the second switching module 124 is electrically connected to a second end of the first energy storage module 122, and a second end of the first switching module 123 is electrically connected to a first end of the second switching module 124.

The first end and the second end of the first energy storage module 122 of the first pulse generating unit 121 are respectively used for being electrically connected to the first end and the second end of the first power source 110.

In any two adjacent first pulse generating units 121, the first end of the first energy storage module 122 of the next first pulse generating unit 121 is electrically connected to the first end of the first switching module 123 of the previous first pulse generating unit 121; the second end of the first energy storage module 122 of the next first pulse generation unit 121 is electrically connected to both the second end of the first switch module 123 of the previous first pulse generation unit 121 and the first end of the second switch module 124.

The output of the last first pulse generating unit 121 is used for electrical connection to the biological tissue.

In some embodiments, as shown in fig. 4 and 6, in the second pulse generating unit 151, a first terminal of a third switching module 153 is electrically connected to a first terminal of the second energy storage module 152, a second terminal of a fourth switching module 154 is electrically connected to a second terminal of the second energy storage module 152, and a second terminal of the third switching module 153 is electrically connected to a first terminal of the fourth switching module 154.

The first end and the second end of the second energy storage module 152 of the first second pulse generating unit 151 are respectively used for being electrically connected with the first end and the second end of the second power source 140.

In any two adjacent second pulse generation units 151, a first end of the second energy storage module 152 of the next second pulse generation unit 151 is electrically connected with a first end of the third switching module 153 of the previous second pulse generation unit 151; the second terminal of the second energy storage module 152 of the second pulse generation unit 151 is electrically connected to the second terminal of the third switching module 153 and the first terminal of the fourth switching module 154 of the second pulse generation unit 151.

The output of the last second pulse generating unit 151 is used for electrical connection to the biological tissue.

Alternatively, referring to fig. 6, the first pulse generating sub-circuit 120 of the embodiment of the present application includes 5 first pulse generating units 121, the second pulse generating sub-circuit 150 includes a second pulse generating unit 151, and accordingly, the number of the first gate driving circuit group 131 and the second gate driving circuit group 161 is set to be 5. As shown in fig. 4 and 6, the switching devices Q1, Q3, Q5, Q7, Q9, Q11, Q13, Q15, Q17, and Q19 are main switches, and Q2, Q4, Q6, Q8, Q10, Q12, Q14, Q16, Q18, and Q20 are tail switches.

Alternatively, the first and

second switching modules

123 and 124 of the first pulse generating unit 121 of the last stage and the third and

fourth switching modules

153 and 154 of the second pulse generating sub-circuit 150 of the last stage may be controlled to be turned on or off by an additional control circuit, and may be controlled by the control unit of the pulse generating device of the embodiment of the present application. Alternatively, the switching devices Q9, Q10, Q19, and Q20 may be controlled to be turned on or off by the control unit. The control terminals of the switching devices Q9, Q10, Q19, and Q20 are electrically connected to the control unit.

In some embodiments, the first pulse generating sub-circuit 120 further includes a first voltage dividing resistor, a first end of the first voltage dividing resistor is electrically connected to the output end of the last first pulse generating unit 121, and a second end of the first voltage dividing resistor is used for electrically connecting to the biological tissue; and/or the presence of a gas in the gas,

the second pulse generating sub-circuit 150 further comprises a second voltage dividing resistor, a first end of the second voltage dividing resistor is electrically connected to the output end of the last second pulse generating unit 151, and a second end of the second voltage dividing resistor is used for electrically connecting to the biological tissue.

Alternatively, referring to fig. 6, R1 represents a first dividing resistor, and R2 represents a second dividing resistor. The output end of the last stage first pulse generating unit 121 of the first pulse generating sub-circuit 120 and the output end of the last stage second pulse generating unit 151 of the second pulse generating sub-circuit 150 are respectively connected to two sides of the load 170, so as to generate high-voltage processing pulses with adjustable positive and negative polarities: the output end of the last stage first pulse generating unit 121 of the first pulse generating sub-circuit 120 and the output end of the last stage second pulse generating unit 151 of the second pulse generating sub-circuit 150 are respectively connected in series with the first voltage dividing resistor R1 and the second voltage dividing resistor R2, and then are electrically connected with two sides of the load 170, so as to output a low-voltage pulse measuring voltage.

Optionally, referring to fig. 6, the first voltage-dividing resistor R1 and the second voltage-dividing resistor R2 are both adjustable resistors, and are electrically connected to the control unit, and the resistances of the first voltage-dividing resistor R1 and the second voltage-dividing resistor R2 are adjusted by the control unit.

Optionally, referring to fig. 6, the first energy storage module 122 includes capacitors, and the capacitor C1, the capacitor C2, the capacitor C3, the capacitor C4, and the capacitor C5 respectively correspond to the capacitors of the first energy storage modules 122 of the first pulse generation units 121 in each stage. The second energy storage module 152 includes capacitors, and the capacitor C6, the capacitor C7, the capacitor C8, the capacitor C9 and the capacitor C10 respectively correspond to the capacitors of the second energy storage modules 152 of the second pulse generation units 151 in each stage.

Alternatively, referring to fig. 6, by controlling the on and off of the switching devices Q1 to Q20, the output of the pulse signals of the first pulse generation stage to the fourth pulse generation stage of the embodiment of the present application can be realized. The specific contents of controlling the on and off of the switch are specifically described in the pulse generating method.

In some embodiments, the first pulse generation sub-circuit 120 and the second pulse generation sub-circuit 150 are configured to, in a third pulse generation phase, form a third pulse generation loop with the biological tissue through the first pulse generation sub-circuit 120 and the second pulse generation sub-circuit 150, and output a first detection pulse signal to the biological tissue; in the third pulse generation circuit, the first energy storage modules 122 of the first pulse generation sub-circuits 120 are all in a parallel charging state, the output voltage of the output end of the last first pulse generation unit 121 is the voltage of the first power supply 110, the first voltage dividing resistor is connected to the third pulse generation circuit, and the biological tissue is grounded through the second pulse generation sub-circuit 150.

In some embodiments, the first pulse generation sub-circuit 120 and the second pulse generation sub-circuit 150 are configured to, during a fourth pulse generation phase, form a fourth pulse generation loop with the biological tissue through the first pulse generation sub-circuit 120 and the second pulse generation sub-circuit 150, and output a second detection pulse signal to the biological tissue; in the fourth pulse generation circuit, the second energy storage modules 152 of the second pulse generation sub-circuits 150 are all in a parallel charging state, the output voltage of the output end of the last second pulse generation unit 151 is the voltage of the second power supply 140, the second voltage-dividing resistor is connected to the fourth pulse generation circuit, and the biological tissue is grounded through the first pulse generation sub-circuit 120; the second detection pulse signal and the first detection pulse signal are pulse signals with opposite polarities; the second detection pulse signal is used to detect a biological property of the biological tissue.

Alternatively, referring to fig. 6, the voltage of the first power source 110 and the voltage of the second power source 140 are both U1, and the first pulse generation sub-circuit 120 includes a resistor R3, a diode D1, a diode D2, a diode D3, and a diode D4. The second pulse generation sub-circuit 150 includes a resistor R4, a diode D5, a diode D6, a diode D7, and a diode D8.

Optionally, the first working pulse signal in the embodiment of the present application is a high-voltage pulse signal, and the first detection pulse signal is a low-voltage measurement signal. Referring to fig. 6, the first pulse generating sub-circuit 120 generates a pulse and applies a high voltage pulse signal to the load 170 through the electrodes, the high voltage pulse signal being applied to the load 170 at a predetermined frequency; the low voltage measuring pulse is applied at intervals of the high voltage pulse signal. A high-voltage pulse signal is applied to the load 170 to realize irreversible electroporation of the cell membrane; a low voltage measurement pulse is applied to the load 170 for real-time acquisition of the impedance spectrum of the load 170.

Alternatively, the first pulse generation sub-circuit 120 and the second pulse generation sub-circuit 150 according to the embodiments of the present application may output bipolar pulse signals. Referring to fig. 6, the output terminals of the last stage pulse generating units of the first pulse generating sub-circuit 120 and the second pulse generating sub-circuit 150 are connected to two ends of a load 170; the direction of the current flow is defined as positive polarity pulses from the upper side to the lower side of the load 170, and negative polarity pulses. The first pulse generation sub-circuit 120 outputs a positive polarity pulse signal, and the second pulse generation sub-circuit 150 outputs a negative polarity pulse signal. The high-voltage pulse signal comprises a positive polarity high-voltage pulse or pulse train, a negative polarity high-voltage pulse or pulse train and a positive and negative bipolar high-voltage pulse or pulse train; the low voltage measuring pulses comprise positive polarity low voltage pulses or pulse trains, negative polarity low voltage pulses or pulse trains, positive and negative bipolar low voltage pulses or pulse trains.

Optionally, the number of stages of the first pulse generating unit 121 and the second pulse generating unit 151 in the embodiment of the present application may be set according to actual needs, and the number of energy storage modules connected in series in a pulse generating circuit in each pulse generating stage may also be actually required to control different switching device conduction designs, so as to output a pulse voltage with a required amplitude.

Based on the same inventive concept, embodiments of the present application provide a pulse generation device, including a pulse generation circuit having a pulse detection function according to any of the embodiments of the present application.

In some embodiments, the pulse generating apparatus further comprises: a control unit.

A control unit for determining a biological property of the biological tissue from the first detection pulse signal, adjusting a pulse parameter of the first detection pulse signal and/or the first working pulse signal according to the biological property of the biological tissue, the pulse parameter comprising at least one of: pulse width, frequency, amplitude.

Optionally, the biological characteristic comprises a load impedance spectrum.

Optionally, the control unit of the embodiment of the present application is configured to measure in real time to obtain a complete time-domain waveform of the voltage and the response current of the first detection pulse signal after the first detection pulse signal is output to the biological tissue, and calculate the load impedance spectrum of the biological tissue. Optionally, the control unit is configured to perform fourier transform on the voltage and current waveforms of the acquired first detection pulse signal respectively to obtain a voltage-current frequency spectrum, so as to obtain a load impedance spectrum of the biological tissue through calculation.

Optionally, the pulse generating circuit can apply low-voltage measuring pulses with continuously-changed pulse widths according to the impedance spectrum frequency band requirement to adjust the frequency spectrum distribution of the pulse waveform; and can continuously detect the impedance spectrum change of the biological tissue within the high-voltage pulse interval time; and can apply designated low-voltage measuring pulses for a plurality of times within the interval time, thereby realizing the real-time detection of the impedance spectrum of the biological tissue.

Optionally, the control unit is further configured to calculate an initial impedance of the biological tissue after applying a preset low-voltage measurement pulse to the load 170 (biological tissue), and check whether the connection from the output terminal of the first pulse generation sub-circuit 120 and/or the second pulse generation sub-circuit 150 to the biological load is correct, and whether there is an open circuit or a short circuit according to the initial impedance value; and judging whether the response current caused by the high-voltage pulse signal to be applied exceeds a preset current protection threshold value of the circuit or not, and adjusting the high-voltage processing pulse parameter according to the judgment result so that the response current does not exceed the preset current protection threshold value of the circuit.

Optionally, the control unit is further configured to determine a category of a pulse signal sent by the first pulse generation sub-circuit 120 and/or the second pulse generation sub-circuit 150 according to the received instruction, control the pulse generation circuit with a pulse detection function according to a preset high-low voltage pulse sequence and the category of the pulse signal, and switch control among pulse generation stages, where each pulse generation stage includes a first pulse generation stage, a second pulse generation stage, a third pulse generation stage, and a fourth pulse generation stage.

The embodiment of the application can adjust the pulse parameters of the first detection pulse signal and/or the first working pulse signal according to the impedance spectrum of the real-time detection biological tissue, so that the design condition is met, and the better ablation effect is achieved.

Based on the same inventive concept, an embodiment of the present application further provides a pulse generating method, which is applied to a pulse generating circuit with a pulse detection function in any embodiment of the present application, and the method includes: step a1 and step a 2.

In step a1, the first control circuit 136 generates a first control signal and transmits the first control signal to each of the first gate drive circuit groups 131.

Step a2, each of the first gate driving circuit groups 131 correspondingly controls the first switch module 123 and the second switch module 124 of each of the first pulse generating units 121 to be turned on or off according to the first control signal, so that the first pulse generating sub-circuit 120 outputs the first working pulse signal or the first detection pulse signal to the biological tissue; the first working pulse signal is used for ablation of target cells of the biological tissue, and the first detection pulse signal is used for detecting a biological property of the biological tissue.

Optionally, the pulse generation method includes:

in the charging phase, the first power source 110 is controlled to charge the first energy storage module 122 of the first pulse generating unit 121 of each stage, and the second power source 140 charges the second energy storage module 152 of the second pulse generating unit 151 of each stage.

Alternatively, referring to fig. 7 in conjunction with the circuit configuration shown in fig. 6, a charging loop is shown, the charging phase comprising:

the switching device Q9, the switching device Q10, the switching device Q19 and the switching device Q20 of the first pulse generation sub-circuit 120 and the second pulse generation sub-circuit 150 are controlled to be turned off; other switching devices are closed and conducted; the capacitor C1 and the capacitor C10 are synchronously charged in parallel to reach a preset charging voltage U1. The charging voltage of the first power supply 110 and the second power supply 140 is U1.

In some embodiments, the first pulse generator sub-circuit 120 outputs a first working pulse signal to the biological tissue, including:

in the first pulse generation phase, the first pulse generation sub-circuit 120 and the second pulse generation sub-circuit 150 form a first pulse generation loop with the biological tissue, and the first pulse generation sub-circuit 120 outputs a first working pulse signal to the biological tissue. In the first pulse generation circuit, the first energy storage modules 122 of the first pulse generation sub-circuit 120 are sequentially connected in series, the second energy storage modules 152 of the second pulse generation sub-circuit 150 are not connected, and the biological tissue is grounded through the second pulse generation sub-circuit 150.

Alternatively, referring to fig. 8, a schematic diagram of the first pulse generation loop of the first pulse generation phase electrically connected to the load 170 is shown. Referring to fig. 8, the switching devices Q1, Q3, Q5, Q7 and Q9 are controlled to be on, the switching devices Q2, Q4, Q6, Q8 and Q10 are controlled to be off, and the capacitors C1 to C5 are connected in series to discharge the load 170, wherein the voltage is N times that of the U1. Meanwhile, the switching devices Q11, Q13, Q15, Q17, and Q19 are controlled to be off, and the switching devices Q12, Q14, Q16, Q18, and Q20 are controlled to be on. The second terminal of the load 170 is connected to ground through switching devices Q12, Q14, Q16, Q18, and Q20, thereby generating positive polarity high voltage pulses of magnitude NU1 (i.e., N U1) on the load 170, N being the number of stages of the pulse generating unit. N is the number of stages corresponding to the pulse generating unit, N =5 in this embodiment, i.e., the voltage is 5U 1.

In some embodiments, the pulse generation method further comprises:

in the second pulse generation stage, the first pulse generation sub-circuit 120 and the second pulse generation sub-circuit 150 form a second pulse generation loop with the biological tissue, and the second pulse generation sub-circuit 150 outputs a second working pulse signal to the biological tissue; in the second pulse generation circuit, the second energy storage modules 152 of the second pulse generation sub-circuit 150 are sequentially connected in series, the first energy storage modules 122 of the first pulse generation sub-circuit 120 are not connected, and the biological tissue is grounded through the first pulse generation sub-circuit 120.

Alternatively, referring to fig. 9, a schematic diagram of the second pulse generation loop of the second pulse generation phase electrically connected to the load 170 is shown. As shown in fig. 9. The switching devices Q1, Q3, Q5, Q7 and Q9 are controlled to be disconnected, the switching devices Q2, Q4, Q6, Q8 and Q10 are controlled to be switched on, the first end of the load 170 is directly connected with the ground, the switching devices Q11, Q13, Q15, Q17 and Q19 are switched on, the switching devices Q12, Q14, Q16, Q18 and Q20 are switched off, the capacitors C6-C10 are connected in series to discharge the load 170, and the amplitude generated on the load 170 is MU 1. M is a negative high voltage pulse corresponding to the number of stages of the pulse generating unit. M =5 in the present embodiment, i.e., the voltage is 5U 1.

Alternatively, as shown in fig. 8, 9 and 12 in combination, the pulse generating circuit with the pulse detection function may output the bipolar high-voltage pulse signal shown in fig. 12 based on the first pulse generating stage and the second pulse generating stage.

In some embodiments, the first pulse generator sub-circuit 120 outputs a first detection pulse signal to the biological tissue, including:

in the third pulse generation stage, the first pulse generation sub-circuit 120, the first voltage divider resistor, the second pulse generation sub-circuit 150 and the biological tissue form a third pulse generation loop, and output a first detection pulse signal to the biological tissue; in the third pulse generation circuit, the first energy storage modules 122 of the first pulse generation sub-circuits 120 are all in a parallel charging state, the output voltage of the output end of the last first pulse generation unit 121 is the voltage of the first power supply 110, the first voltage dividing resistor is connected to the third pulse generation circuit, and the biological tissue is grounded through the second pulse generation sub-circuit 150.

Alternatively, referring to fig. 10, a schematic diagram of the third pulse generation loop of the third pulse generation phase electrically connected to the load 170 is shown. The switching devices Q1, Q3, Q5, Q7 and Q9 are controlled, the switching devices Q11, Q13, Q15, Q17 and Q19 are turned off, the switching devices Q2, Q4, Q6, Q8, Q12, Q14, Q16, Q18 and Q20 are turned on, and all capacitors are in a parallel charging state at this time. Unlike the charging phase, the Q10 is turned off, the charging voltage U1 may form a discharging loop through the first voltage dividing resistor R1, the load 170 and the switching devices Q12, Q14, Q16, Q18, and Q20, a low-voltage positive polarity pulse with a voltage amplitude U1 × ZL/(ZL + R1) is generated on the load 170, and the resistance of the load 170 is ZL. The low-voltage positive-polarity pulse amplitude can be adjusted through the first voltage dividing resistor R1, and the low-voltage pulse width can be controlled through on-off adjustment of the switching device Q20.

Optionally, the first pulse generating sub-circuit 120 outputs a first detection pulse signal to the biological tissue, including:

in the fourth pulse generation phase, the first pulse generation sub-circuit 120, the second pulse generation sub-circuit 150 and the biological tissue form a fourth pulse generation loop, and a second detection pulse signal is output to the biological tissue; in the fourth pulse generation circuit, the second energy storage modules 152 of the second pulse generation sub-circuits 150 are all in a parallel charging state, the output voltage of the output end of the last second pulse generation unit 151 is the voltage of the second power supply 140, the second voltage-dividing resistor is connected to the fourth pulse generation circuit, and the biological tissue is grounded through the first pulse generation sub-circuit 120; the second detection pulse signal and the first detection pulse signal are pulse signals with opposite polarities; the second detection pulse signal is used to detect a biological characteristic of the biological tissue.

Alternatively, referring to fig. 11, a schematic diagram of the fourth pulse generation loop of the fourth pulse generation phase electrically connected to the load 170 is shown. The switching devices Q1, Q3, Q5, Q7, Q9, Q11, Q13, Q15, Q17 and Q19 are controlled to be turned off, the switching devices Q2, Q4, Q6, Q8, Q10, Q12, Q14, Q16 and Q18 are controlled to be turned on, the switching device Q20 is controlled to be turned off, all capacitors are in a parallel charging state, a charging voltage U1 of the second pulse generation sub-circuit 150 can form a discharging loop through the second voltage dividing resistor R2, the load 170 and the switching devices Q2, Q4, Q6, Q8 and Q10, and low-voltage negative polarity pulses with the voltage amplitude of U1 × ZL/(ZL + R2) are generated on the load 170. The low-voltage negative polarity pulse amplitude can be adjusted through the second voltage-dividing resistor R2, and the low-voltage pulse width can be adjusted through adjusting the on-off control of the switching device Q10, i.e., adjusting the on-time of the switching device Q10.

Alternatively, as shown in fig. 10, 11, 13 and 14, based on the third pulse generation phase and the fourth pulse generation phase, the pulse generation circuit with pulse detection function may output the low voltage measurement pulse signal as shown in fig. 13 and 14, where fig. 13 is a low voltage measurement pulse with a lower amplitude, and fig. 14 is a low voltage measurement pulse with a higher amplitude.

Alternatively, based on the pulse generating circuit with pulse detection function shown in fig. 6, the first pulse generating sub-circuit 120 and the second pulse generating sub-circuit 150 are positive and negative Marx main circuits, respectively. The pulse generation method comprises the following steps:

the method comprises the following steps: the connecting circuit connects the output end of the last-stage pulse generating unit of the positive and negative Marx main circuits to the two ends of the load 170; defining the current direction to be positive polarity pulse from the upper side to the lower side of the load 170, otherwise to be negative polarity pulse; controlling the switching devices Q9, Q10, Q19 and Q20 of the positive and negative Marx main circuits to be switched off; other charging switches are closed and conducted; at this time, the capacitors C1-C10 of the positive and negative Marx main circuits are synchronously charged in parallel to reach the preset charging voltage U1, which is shown in FIG. 7.

Step two: whether the Marx main circuit can normally charge and discharge is checked; if the normal charging and discharging work can be carried out, the following process is continued; if not, an alarm is given and the process is exited; applying preset low-voltage pulses to two ends of the load 170, calculating the initial impedance of the biological tissue, and checking whether the connection between the output end of the Marx main circuit and the load 170 is correct or not and whether the conditions of open circuit or short circuit exist or not according to the initial impedance value; judging whether the response current caused by the high-voltage pulse to be applied exceeds a preset current protection threshold value of the circuit or not, and further optimizing and adjusting the high-voltage processing pulse parameters according to the current protection threshold value; and judging the type of the pulse signal sent by the Marx main circuit according to the received user instruction, entering the following corresponding steps, and switching among the following steps according to a preset high-low voltage pulse sequence.

Step three: if it is necessary to apply a positive polarity low voltage measurement pulse; the switching devices Q1, Q3, Q5, Q7, Q9, Q11, Q13, Q15, Q17 and Q19 are controlled to be turned off, and Q2, Q4, Q6, Q8, Q12, Q14, Q16, Q18 and Q20 are controlled to be turned on, and all capacitors are in a parallel charging state at this time. The only difference is that when Q10 is turned off, the charging voltage U0 may form a discharging loop through the first voltage dividing resistor R1, the load 170 and the switching devices Q12, Q14, Q16, Q18, Q20, generating a low voltage positive polarity pulse with a voltage amplitude U1 × ZL/(ZL + R1) on the load 170. The low-voltage positive polarity pulse amplitude can be adjusted by the first voltage dividing resistor R1, and the low-voltage pulse width can be controlled by adjusting the on/off of the switching device Q20, as shown in fig. 10.

Step four: if it is desired to apply a negative polarity low voltage measurement pulse; the switching devices Q1, Q3, Q5, Q7, Q9, Q11, Q13, Q15, Q17 and Q19 are controlled to be turned off, the switching devices Q2, Q4, Q6, Q8, Q10, Q12, Q14, Q16 and Q18 are turned on, Q20 is turned off, all capacitors are in a parallel charging state, the negative Marx main circuit charging voltage U1 can form a discharging loop through the second voltage dividing resistor R2, the load 170 and the switching devices Q2, Q4, Q6, Q8 and Q10, and low-voltage negative polarity pulses with the voltage amplitude U1 × ZL/(ZL + R2) are generated on the load 170. The amplitude of the low-voltage negative polarity pulse can be adjusted by the second voltage-dividing resistor R2, and the width of the low-voltage pulse can be controlled by adjusting the on/off of the switching device Q10, as shown in fig. 11.

Step five: if a positive polarity high voltage processing pulse is required to be applied; controlling the switching devices Q1, Q3, Q5, Q7 and Q9 to be turned on, the switching devices Q2, Q4, Q6, Q8 and Q10 to be turned off, and the capacitors C1-C5 to be connected in series to discharge the biological load, wherein the voltage is NU0, N is the number of stages corresponding to the pulse generating unit, and N =5 in the embodiment; meanwhile, the switching devices Q11, Q13, Q15, Q17, Q19 are turned off, and the switching devices Q12, Q14, Q16, Q18, Q20 are turned on. Therefore, the lower end of the load 170 is connected to ground through the switches Q12, Q14, Q16, Q18, Q20, thereby generating positive polarity high voltage pulses having an amplitude NU1 and N corresponding to the number of stages of the pulse generating unit on the load, as shown in fig. 8.

Step six: if a negative polarity high voltage processing pulse is required to be applied; the switching devices Q1, Q3, Q5, Q7, Q9 are controlled to be turned off, the switching devices Q2, Q4, Q6, Q8, Q10 are turned on, so that the upper side of the load 170 is directly connected to the ground, the switching devices Q11, Q13, Q15, Q17, Q19 are turned on, Q12, Q14, Q16, Q18, Q20 are turned off, and the capacitors C6-C10 are connected in series to discharge the load 170, so that high voltage pulses of negative polarity having amplitudes MU1 and M corresponding to the number of stages of the pulse generating unit are generated on the bio-load, as shown in fig. 9.

Step seven: calculating the impedance spectrum of the biological tissue: and measuring in real time to obtain complete time domain waveforms of the voltage and the response current of the low-voltage pulse, and performing Fourier transform on the voltage and current waveforms of the collected low-voltage pulse to obtain a voltage-current frequency spectrum, so that the load impedance spectrum of the biological tissue is obtained by calculation.

Those of skill in the art will appreciate that the various operations, methods, steps in the processes, acts, or solutions discussed in this application can be interchanged, modified, combined, or eliminated. Further, other steps, measures, or schemes in various operations, methods, or flows that have been discussed in this application can be alternated, altered, rearranged, broken down, combined, or deleted. Further, steps, measures, schemes in the prior art having various operations, methods, procedures disclosed in the present application may also be alternated, modified, rearranged, decomposed, combined, or deleted.

The terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless otherwise specified.

The particular features, structures, materials, or characteristics may be combined in any suitable manner in any one or more embodiments or examples.

It should be understood that, although the steps in the flowcharts of the figures are shown in order as indicated by the arrows, the steps are not necessarily performed in order as indicated by the arrows. The steps are not performed in the exact order shown and may be performed in other orders unless otherwise indicated herein. Moreover, at least a portion of the steps in the flow chart of the figure may include multiple sub-steps or multiple stages, which are not necessarily performed at the same time, but may be performed at different times, which are not necessarily performed in sequence, but may be performed alternately or alternately with other steps or at least a portion of the sub-steps or stages of other steps.

The foregoing is only a partial embodiment of the present application, and it should be noted that, for those skilled in the art, several modifications and decorations can be made without departing from the principle of the present application, and these modifications and decorations should also be regarded as the protection scope of the present application.

Claims

1. A pulse generating circuit having a pulse detection function, comprising: a first pulse generation sub-circuit and a first drive circuit;

the first driving circuit comprises a first control circuit, a first lead and at least one first gate pole driving circuit group, the first control circuit is in magnetic induction connection with the first gate pole driving circuit groups through the first lead, and each first gate pole driving circuit group is correspondingly and electrically connected with a first switch module and a second switch module of one first pulse generation unit;

the first control circuit is used for generating a first control signal and transmitting the first control signal to each first gate pole driving circuit group, so that each first gate pole driving circuit group correspondingly controls the connection or disconnection of the first switch module and the second switch module of each first pulse generation unit, and the first pulse generation sub-circuit outputs a first working pulse signal or a first detection pulse signal to biological tissues; the first working pulse signal is used for ablation of target cells of the biological tissue, and the first detection pulse signal is used for detecting biological characteristics of the biological tissue;

the first control circuit comprises a first half-bridge control circuit, a first magnetic drive signal generator and a first signal control power supply;

the first signal control power supply is electrically connected with the first half-bridge control circuit and used for outputting a second signal to the first half-bridge control circuit;

the first half-bridge control circuit is used for converting the second signal into a first control signal under the control of the first signal and controlling each first gate pole driving circuit group to transmit the first control signal through the first lead, and the first control signal is a bipolar pulse signal;

the first gate drive circuit group comprises a first gate modulation circuit and a second gate modulation circuit;

the second gate modulation circuit is correspondingly and electrically connected with a second switch module of the first pulse generation unit and controls the second switch module to be switched on or off;

the first gate pole modulation circuit comprises a first input end, a first MOSFET, a second MOSFET, a first driving resistor, a second driving resistor, a first gate pole capacitor, a first transient suppression diode, a first divider resistor and a first output end; the first input end receives the first control signal transmitted by the first half-bridge control circuit through the first wire, one pin of the first input end is connected with the S pole of the first MOSFET, and the other pin of the first input end is connected with the S pole of the second MOSFET; the G pole of the first MOSFET is connected with the S pole of the second MOSFET through the first driving resistor; the G pole of the second MOSFET is connected with the D pole of the first MOSFET through the second driving resistor; the first gate electrode capacitor is connected between the D pole of the first MOSFET and the D pole of the second MOSFET in series; one pin of the first output end is connected with one end of a first transient suppression diode and is connected with the D pole of the second MOSFET through the first divider resistor; the other pin of the first output end is connected with the other end of the first transient suppression diode and is simultaneously connected with the D pole of the first MOSFET; and/or the presence of a gas in the gas,

the second gate modulation circuit comprises a second input end, a third MOSFET, a fourth MOSFET, a third driving resistor, a fourth driving resistor, a second gate capacitor, a second instantaneous suppression diode, a second divider resistor and a second output end; the second input end receives the first control signal transmitted by the first half-bridge control circuit through the first wire, one pin of the second input end is connected with the S pole of the third MOSFET, and the other pin of the second input end is connected with the S pole of the fourth MOSFET; the G pole of the third MOSFET is connected with the S pole of the fourth MOSFET through the third driving resistor; the G pole of the fourth MOSFET is connected with the D pole of the third MOSFET through the fourth driving resistor; the second gate capacitor is connected in series between the D pole of the third MOSFET and the D pole of the fourth MOSFET; one pin of the second output end is connected with one end of the second transient suppression diode and is connected with the D pole of the fourth MOSFET through the second divider resistor; and the other pin of the second output end is connected with the other end of the second transient suppression diode and is simultaneously connected with the D pole of the third MOSFET.

2. The pulse generating circuit with pulse detection function according to claim 1, wherein in the first pulse generating unit, a first end of the first switching module is electrically connected to a first end of the first energy storage module, a second end of the second switching module is electrically connected to a second end of the first energy storage module, and a second end of the first switching module is electrically connected to a first end of the second switching module;

in any two adjacent first pulse generation units, the first end of the first energy storage module of the latter first pulse generation unit is electrically connected with the first end of the first switch module of the former first pulse generation unit; the second end of the first energy storage module of the latter first pulse generation unit is electrically connected with the second end of the first switch module of the former first pulse generation unit and the first end of the second switch module;

and the output end of the last first pulse generating unit is used for being electrically connected with the biological tissue.

3. The pulse generating circuit with a pulse detecting function according to claim 2, further comprising: a second pulse generating sub-circuit and a second driving circuit;

the second driving circuit comprises a second control circuit, a second lead and at least one second gate electrode driving circuit group, the second control circuit is in magnetic induction connection with the second gate electrode driving circuit groups through the second lead, and each second gate electrode driving circuit group is correspondingly and electrically connected with a third switch module and a fourth switch module of one second pulse generation unit;

the second control circuit is configured to generate a second control signal and transmit the second control signal to each second gate driving circuit group, so that each second gate driving circuit group correspondingly controls on or off of a fourth switch module and a fourth switch module of each second pulse generation unit.

4. The pulse generating circuit with pulse detecting function according to claim 3, wherein the first pulse generating sub-circuit and the second pulse generating sub-circuit are configured to form a first pulse generating circuit with the biological tissue in a first pulse generating phase, and the first pulse generating sub-circuit outputs the first working pulse signal to the biological tissue; in a second pulse generation stage, the first pulse generation sub-circuit and the second pulse generation sub-circuit form a second pulse generation loop with the biological tissue, and the second pulse generation sub-circuit outputs a second working pulse signal to the biological tissue; the second working pulse signal is used for ablation of target cells of the biological tissue, and the first working pulse signal and the second working pulse signal are pulse signals with opposite polarities.

5. The pulse generating circuit with the pulse detection function according to claim 4, wherein in the second pulse generating unit, a first end of the third switching module is electrically connected with a first end of the second energy storage module, a second end of the fourth switching module is electrically connected with a second end of the second energy storage module, and a second end of the third switching module is electrically connected with a first end of the fourth switching module;

the first end and the second end of the second energy storage module of the first pulse generation unit are respectively used for being electrically connected with the first end and the second end of a second power supply;

in any two adjacent second pulse generation units, the first end of the second energy storage module of the latter second pulse generation unit is electrically connected with the first end of the third switch module of the former second pulse generation unit; the second end of the second energy storage module of the second pulse generation unit is electrically connected with the second end of the third switch module of the second pulse generation unit and the first end of the fourth switch module;

and the output end of the last second pulse generating unit is used for being electrically connected with the biological tissue.

6. The pulse generating circuit with pulse detecting function as claimed in claim 5, wherein said first pulse generating sub-circuit further comprises a first voltage dividing resistor, a first end of said first voltage dividing resistor is electrically connected to an output end of a last said first pulse generating unit, and a second end of said first voltage dividing resistor is electrically connected to said biological tissue; and/or the presence of a gas in the atmosphere,

7. The pulse generating circuit with pulse detection function according to claim 6, wherein the first pulse generating sub-circuit and the second pulse generating sub-circuit are configured to form a third pulse generating circuit with the biological tissue in a third pulse generating stage, and output the first detection pulse signal to the biological tissue; in the third pulse generation circuit, the first energy storage modules of the first pulse generation sub-circuits are all in a parallel charging state, the output voltage of the output end of the last first pulse generation unit is the voltage of the first power supply, the first voltage dividing resistor is connected to the third pulse generation circuit, and the biological tissue is grounded through the second pulse generation sub-circuit.

8. The pulse generating circuit with pulse detection function according to claim 7, wherein the first pulse generating sub-circuit and the second pulse generating sub-circuit are configured to form a fourth pulse generating circuit with the biological tissue in a fourth pulse generating stage, and output a second detection pulse signal to the biological tissue; in the fourth pulse generation circuit, the second energy storage modules of the second pulse generation sub-circuits are all in a parallel charging state, the output voltage of the output end of the last second pulse generation unit is the voltage of the second power supply, the second voltage-dividing resistor is connected to the fourth pulse generation circuit, and the biological tissue is grounded through the first pulse generation sub-circuit; the second detection pulse signal and the first detection pulse signal are pulse signals with opposite polarities; the second detection pulse signal is used for detecting a biological characteristic of the biological tissue.

9. The pulse generating circuit with pulse detection function according to claim 4, wherein the second control circuit comprises a second half-bridge control circuit, a second magnetic drive signal generator and a second signal control power supply;

the second half-bridge control circuit is configured to convert the fourth signal into a second control signal under the control of the third signal, and control each second gate driving circuit group to transmit the second control signal through the second conducting wire, where the second control signal is a bipolar pulse signal.

10. A pulse generating apparatus comprising a pulse generating circuit having a pulse detecting function according to any one of claims 1 to 9.

11. The pulse generating apparatus of claim 10, further comprising: a control unit;

the control unit is used for determining a biological characteristic of the biological tissue according to the first detection pulse signal, and adjusting a pulse parameter of the first detection pulse signal and/or the first working pulse signal according to the biological characteristic of the biological tissue, wherein the pulse parameter comprises at least one of the following items: pulse width, frequency, amplitude.

12. A pulse generating method applied to a pulse generating circuit with a pulse detecting function according to any one of claims 1 to 9, comprising:

the first control circuit generates a first control signal and transmits the first control signal to each first gate electrode driving circuit group;

each first gate driving circuit group correspondingly controls the connection or disconnection of the first switch module and the second switch module of each first pulse generation unit according to the first control signal, so that the first pulse generation sub-circuit outputs a first working pulse signal or a first detection pulse signal to biological tissues; the first working pulse signal is used for ablation of target cells of the biological tissue, and the first detection pulse signal is used for detecting biological characteristics of the biological tissue.

13. A method of pulse generation as claimed in claim 12, wherein the pulse generation circuit comprises a first pulse generation sub-circuit and a second pulse generation sub-circuit;

the first pulse generating sub-circuit outputs a first working pulse signal to a biological tissue, including:

in a first pulse generation phase, the first pulse generation sub-circuit and the second pulse generation sub-circuit form a first pulse generation circuit with the biological tissue, and the first pulse generation sub-circuit outputs the first working pulse signal to the biological tissue; in the first pulse generation loop, the first energy storage modules of the first pulse generation sub-circuit are sequentially connected in series, the second energy storage modules of the second pulse generation sub-circuit are not connected, and the biological tissue is grounded through the second pulse generation sub-circuit.

14. A method of pulse generation as claimed in claim 12, wherein the pulse generation circuit comprises a first pulse generation sub-circuit and a second pulse generation sub-circuit;

the first pulse generating sub-circuit outputs a first detection pulse signal to a biological tissue, including:

in a third pulse generation phase, the first pulse generation sub-circuit, the first voltage-dividing resistor, the second pulse generation sub-circuit and the biological tissue form a third pulse generation circuit, and the first detection pulse signal is output to the biological tissue; in the third pulse generation circuit, the first energy storage modules of the first pulse generation sub-circuits are all in a parallel charging state, the output voltage of the output end of the last first pulse generation unit is the voltage of a first power supply, the first divider resistor is connected to the third pulse generation circuit, and the biological tissue is grounded through the second pulse generation sub-circuit.

15. A method of pulse generation as claimed in claim 12, wherein the pulse generation circuit comprises a first pulse generation sub-circuit and a second pulse generation sub-circuit;

the pulse generation method further comprises at least one of:

in a fourth pulse generation stage, the first pulse generation sub-circuit, the second pulse generation sub-circuit and the biological tissue form a fourth pulse generation loop, and a second detection pulse signal is output to the biological tissue; in the fourth pulse generation circuit, the second energy storage modules of the second pulse generation sub-circuits are all in a parallel charging state, the output voltage of the output end of the second pulse generation unit of the last second pulse generation sub-circuit is the voltage of a second power supply, the second voltage division resistor is connected into the fourth pulse generation circuit, and the biological tissue is grounded through the first pulse generation sub-circuit; the second detection pulse signal and the first detection pulse signal are pulse signals with opposite polarities; the second detection pulse signal is used for detecting a biological feature of the biological tissue.