CN114085776A

CN114085776A - Adherent cell culture system

Info

Publication number: CN114085776A
Application number: CN202111406469.1A
Authority: CN
Inventors: 张锴; 宋志兵; 蔡罗强; 吴定; 冯振雄; 丁正科; 张舒乐
Original assignee: Hunan Kaiqi Shidai Biotechnology Co ltd
Current assignee: Hunan Kaiqi Shidai Biotechnology Co ltd
Priority date: 2021-11-24
Filing date: 2021-11-24
Publication date: 2022-02-25

Abstract

The invention relates to an adherent cell culture system, comprising: the device comprises a supporting table, a culture box device embedded in the supporting table, a culture dish device supported on the supporting table, a touch display device, a control unit, a container bearing table and an interface unit; the culture dish device comprises: the device comprises a feeding unit, a collecting unit, a cell culture dish, a main pipeline, a first pipe pressure valve and a peristaltic pump; the cell culture dish is positioned in the incubator device; the collecting unit is connected with the container bearing platform; the first pipe pressure valve and the peristaltic pump are arranged on the main pipeline at intervals; the feeding unit is connected with the main pipeline, and the connecting position of the feeding unit and the main pipeline is positioned at the upstream of the first pipe pressure valve; the collecting unit is connected with the main pipeline, and the connecting position of the collecting unit and the main pipeline is positioned between the first pipe pressure valve and the peristaltic pump; the end part of the main pipeline positioned at the downstream of the peristaltic pump penetrates through the incubator device and is integrally arranged with the end cover of the cell culture dish, and the end surface of the end part does not exceed the inner side surface of the end cover.

Description

Adherent cell culture system

Technical Field

The invention relates to an adherent cell culture system.

Background

Cell culture is a method for researching animal cell behaviors, which is established in the beginning of the twentieth century, and is characterized in that cells are taken out from tissues in vivo, the in vivo growth environment is simulated, the cells are grown and propagated under the conditions of sterility, proper temperature, pH value and nutrition, and the structure and the functions of the cells are maintained. Cell preparation refers to a process in which scientific researchers culture a high-purity target cell end product by using a relatively mature cell culture scheme, and the high-purity target cell end product can be directly applied to scientific research and clinical fields after function and quality detection.

The operations of feeding, draining and the like in the cell preparation process are very important, the feeding and draining operations are usually realized in a manual operation mode in the cell preparation process at present, and the operation process is usually realized by opening a cell culture dish, so that the environment in the culture dish is easily polluted, and the cell culture efficiency is seriously influenced.

Disclosure of Invention

The invention aims to provide an adherent cell culture system.

In order to achieve the above object, the present invention provides an adherent cell culture system comprising: a support table, a incubator device embedded in the support table, a culture dish device supported on the support table, a touch display device, a control unit, a container carrying table and an interface unit;

the culture dish device comprises: the device comprises a feeding unit, a collecting unit, a cell culture dish, a main pipeline, a first pipe pressure valve and a peristaltic pump;

the cell culture dish is positioned in the incubator assembly;

the collecting unit is connected with the container bearing table;

the first pipe pressure valve and the peristaltic pump are arranged on the main pipeline at intervals;

the feeding unit is connected with the main pipeline, and the connection position of the feeding unit and the main pipeline is positioned at the upstream of the first pipe pressure valve;

the collecting unit is connected with the main pipeline, and the connecting position of the collecting unit and the main pipeline is positioned between the first pipe pressure valve and the peristaltic pump;

the end part of the main pipeline, which is positioned at the downstream of the peristaltic pump, penetrates through the incubator device and is integrally arranged with the end cover of the cell culture dish, and the end surface of the end part does not exceed the inner side surface of the end cover.

According to one aspect of the invention, the feed unit comprises: the device comprises a plurality of first containers for storing nutrient solution, a plurality of second containers for storing enzyme, a first branch pipe connected with the first containers, a second branch pipe connected with the second containers, and branch pipe pressure valves respectively arranged on the first branch pipe and the second branch pipe;

on the main pipeline, the first branch pipes and the second branch pipes are arranged in a staggered mode at intervals;

the collecting unit includes: the collecting device comprises a plurality of collecting containers, collecting branch pipes connected with the collecting containers, collecting pipes connected with the main pipeline, and collecting pipe pressure valves respectively arranged on the collecting branch pipes;

one end of the collecting branch pipe is connected with the collecting container, and the other end of the collecting branch pipe is connected with the collecting pipe;

the collection container comprises a collection container cover and a collection container body detachably connected with the collection container cover;

the end of the collecting branch pipe is integrally arranged with the collecting container cover.

According to an aspect of the invention, the cell culture dish further comprises: the bottle body is detachably connected with the end cover;

the bottle body includes: a body portion and a neck portion provided at one side of the body portion;

the bottom part of one end of the bottle body part, which is connected with the bottle neck part, is an inclined plane, and the rest part is a plane;

support legs are arranged at the edges of the inclined surfaces;

the bottom ends of the supporting legs are flush with the plane of the bottom of the bottle body part;

the end cover or the bottle body is provided with a medical breathable filter membrane for ventilating and blocking liquid.

According to one aspect of the invention, the incubator assembly comprises: the cell culture dish support device comprises a box body with a heating function, a box cover, a temperature and humidity adjusting device, a culture dish support table, a microscope device and an illuminating device, wherein the temperature and humidity adjusting device is arranged on one side of the box body and is positioned on the outer side of the box body;

meshes penetrating through the side wall of the box body are regularly arranged on one side of the box body connected with the temperature and humidity adjusting device;

the mesh is positioned above the culture dish supporting table;

the temperature and humidity adjusting device is used for performing gas exchange with the inside of the box body through the mesh and assisting in adjusting the temperature and humidity in the box body.

According to one aspect of the invention, the upper end of the box body is provided with an annular sealing heat insulation strip;

the cross section of the annular sealing heat insulation strip is semicircular or semielliptical;

at least one annular groove coaxial with the annular sealing heat-insulating strip is arranged on one side, away from the box body, of the annular sealing heat-insulating strip;

the side edge of the annular sealing heat insulation strip corresponding to the position where the box body is rotatably connected with the box cover is provided with a notch;

the main pipeline is connected with the end cover through the notch.

According to one aspect of the invention, the culture dish support table comprises: the cell culture dish comprises a connecting seat supported at the bottom of the box body, a lifting seat connected with the connecting seat, a driving device and a detection device for detecting the internal state of the cell culture dish;

the driving device is supported on the connecting seat;

one end of the lifting seat is a rotating connecting end which is rotatably connected with the connecting seat, and the other end of the lifting seat is a lifting connecting end which is connected with the driving device;

under the driving action of the driving device, the lifting connecting end can do lifting motion relative to the connecting seat.

According to an aspect of the present invention, the connection socket includes: the connecting frame body and the bottom support are arranged on the lower side of the connecting frame body;

the lifting seat is positioned on the inner side of the connecting frame body and above the bottom support;

the lifting seat is of a frame structure, and a vessel mounting position for mounting the cell culture vessel is arranged on the lifting seat;

the vessel mounting station comprises: a first hollow portion and a second hollow portion communicating with each other;

the second hollow part is provided with an opening at the end part of the rotating connecting end of the lifting seat;

in the width direction of the lifting seat, the lifting seat is provided with penetrating light-transmitting channels at two opposite sides of the vessel mounting position;

the light-transmitting channel is arranged at the position of the first hollow part close to the lifting connecting end of the lifting seat.

According to an aspect of the present invention, the driving device includes: the driver is used for outputting linear displacement, and the fork arm is connected with the driver;

the yoke comprises: a driver connecting arm and a lifting seat connecting arm;

the connecting arm of the lifting seat is perpendicular to the connecting arm of the driver;

a linear channel is arranged on the bottom support;

one end of the lifting seat connecting arm, which is far away from the driver connecting arm, penetrates through the linear channel and is hinged with the lifting connecting end of the lifting seat;

the lifting seat connecting arm is positioned at the lower side of the bottom support, and one side, which is far away from the driver connecting arm, is hinged with the movable end of the driver;

a rubber damping structure is arranged at the hinged position of the driver connecting arm and the driver and is used for silent rotation of the hinged position;

the driver connecting arm is embedded with a position sensor for detecting the moving position of the driver connecting arm and a limiting structure for limiting the maximum displacement of the driver connecting arm.

According to one aspect of the invention, the microscopy apparatus comprises: the focusing unit is provided with an optical lens and a microscopic imaging unit for receiving an image in the optical lens;

the focusing unit is arranged on the bottom support of the connecting seat;

the microscopic imaging unit is arranged at the bottom of the box body and is positioned at the outer side of the box body;

and the gear of the focusing unit is arranged at the bottom of the box body in a driving manner, and the gear is positioned at the outer side of the box body in a driving manner.

According to an aspect of the invention, the interface unit comprises: the device comprises a power supply interface for connecting a power supply, an oxygen interface for connecting an external oxygen source, a carbon dioxide interface for connecting an external carbon dioxide source, a nitrogen interface for connecting an external nitrogen source, a safety structure and a switch structure;

the control unit is respectively connected with the incubator device, the culture dish device, the touch display device and the interface unit.

According to one scheme of the invention, the adherent cell culture system can realize automatic operation of a cell culture process without manual participation. Meanwhile, the system can visually display the running states of all parts through the touch display device, effectively ensures that a user comprehensively grasps the running process of the system and the cell culture process, and ensures high efficiency and convenience of cell culture.

According to a scheme of the invention, the incubator device and the container bearing table are arranged on the front side of the self-supporting table of the system, so that the cell culture process can be conveniently checked and operated by a user, and the user experience is effectively improved.

According to one scheme of the invention, the cell culture dish realizes the closed construction of the cell culture dish unit by combining the structures of the feeding unit, the collecting unit, the cell culture dish, the pipeline and the like, so that the whole cell culture process can be effectively realized as long as the cleanliness of the whole culture system is ensured, no extra manual work is needed, and the risk of the pollution of the culture dish caused by the manual work is effectively avoided. In addition, the structure of the invention also effectively realizes the automation in the cell culture process, and further effectively improves the culture efficiency.

According to one scheme of the invention, the cover body and the pipeline of the cell culture dish are directly and integrally arranged, so that the problem that the sealing property between the pipeline and the cover body is difficult to solve during system assembly is effectively solved, and the risk that the joint position of the pipeline and the cover body is easily corroded by pollutants is also effectively avoided. In addition, the structure that the pipeline and the cover body are integrally arranged further facilitates the connection with the bottle body of the culture dish, effectively eliminates the contact to the inside of the culture dish in the connection process, greatly ensures the cleanliness of the whole system and is beneficial to ensuring the normal operation of the whole period of the cell culture process.

According to one embodiment of the invention, the whole sealing performance is excellent, and the invention is beneficial to ensuring the stable and clean inside of the whole culture dish.

Drawings

FIG. 1 is a perspective view schematically illustrating an adherent cell culture system according to one embodiment of the present invention;

FIG. 2 is a front view schematically illustrating an adherent cell culture system according to one embodiment of the present invention;

FIG. 3 is a rear view schematically showing an adherent cell culture system according to one embodiment of the present invention;

FIG. 4 is a block diagram schematically illustrating a culture dish arrangement according to one embodiment of the present invention;

FIG. 5 is a view schematically showing the structure of a cell culture dish according to an embodiment of the present invention;

FIG. 6 is a perspective view schematically showing an incubator assembly according to one embodiment of the present invention;

FIG. 7 is a view schematically showing the internal structure of an incubator assembly according to an embodiment of the present invention;

FIG. 8 is a plan view schematically showing a incubator assembly according to an embodiment of the present invention;

FIG. 9 is a bottom view schematically illustrating an incubator assembly according to one embodiment of the present invention;

FIG. 10 is a block diagram schematically illustrating an annular sealing and insulating collar, in accordance with one embodiment of the present invention;

FIG. 11 is a view schematically showing the arrangement of a culture dish support table and a microscope device according to an embodiment of the present invention;

FIG. 12 is a side view schematically showing a culture dish support table according to an embodiment of the present invention;

FIG. 13 is a schematic view showing a structure of a dish support table according to an embodiment of the present invention;

FIG. 14 is a bottom view schematically illustrating a culture dish support table according to an embodiment of the present invention;

fig. 15 is a structural view schematically showing a lifting base according to an embodiment of the present invention.

Detailed Description

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments will be briefly described below. It is obvious that the drawings in the following description are only some embodiments of the invention, and that for a person skilled in the art, other drawings can be derived from them without inventive effort.

In describing embodiments of the present invention, the terms "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in an orientation or positional relationship that is based on the orientation or positional relationship shown in the associated drawings, which is for convenience and simplicity of description only, and does not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus, the above-described terms should not be construed as limiting the present invention.

The present invention is described in detail below with reference to the drawings and the specific embodiments, which are not repeated herein, but the embodiments of the present invention are not limited to the following embodiments.

Referring to fig. 1, 2 and 3, according to one embodiment of the present invention, an adherent cell culture system of the present invention comprises: a support table 1, a incubator device 2 embedded in the support table 1, a petri dish device 3 supported on the support table 1, a touch display device 4, a control unit, an interface unit 6 and a container table 5. In the present embodiment, the control unit is connected to the incubator device 2, the culture dish device 3, the touch display device 4 and the interface unit 6, wherein the control unit can control the incubator device 2 and the culture dish device 3 respectively through information input by the touch display device 4, and can display information collected in the incubator device 2 and the culture dish device 3 through the touch display device 4.

Through the arrangement, the adherent cell culture system can realize the automatic operation of the cell culture process without manual participation. Meanwhile, the system can visually display the running states of all parts through the touch display device, effectively ensures that a user comprehensively grasps the running process of the system and the cell culture process, and ensures high efficiency and convenience of cell culture.

Referring to fig. 1, 2 and 3, according to one embodiment of the present invention, the support base 1 is a hollow structure, and the incubator unit 2 and the container platform 5 are provided at a position near the front side of the support base 1, and the support rod and the touch display unit 4 are provided at a position near the rear side of the support base 1. In this embodiment, the support rods are used to support the supply unit 31 in the culture dish device 3 for stable downward liquid delivery. In the present embodiment, the support bar and the touch display device 4 are arranged side by side on the support base 1. In the present embodiment, the control unit is located inside the support table 1. The interface unit 6 is located at the lower portion of the rear side of the support table 1.

Through the arrangement, the incubator device 2 and the container bearing table 5 are arranged from the front side of the supporting table 1, so that the cell culture process can be checked and operated by a user conveniently, and the user experience is effectively improved.

Referring to fig. 1, 2, 3 and 4, according to an embodiment of the present invention, the culture dish assembly 3 includes: a supply unit 31, a collection unit 32, a cell culture dish 33, a main line 34, a first tube pressure valve 35 and a peristaltic pump 36. In this embodiment, the cell culture dish 33 is located in the incubator assembly 2; the collecting unit 32 is connected to the container carrier table 5. In the present embodiment, the first pipe pressure valve 35 and the peristaltic pump 36 are provided on the main line 34 at intervals. The supply unit 31 is connected to the main pipeline 34, and the connection position of the supply unit 31 to the main pipeline 34 is located upstream of the first pipe pressure valve 35; the collecting unit 32 is connected to the main line 34, the position where the collecting unit 32 is connected to the main line 34 is located downstream of the first pipe pressure valve 35, and the position where the collecting unit 32 is connected to the main line 34 is located between the first pipe pressure valve 35 and the peristaltic pump 36. In this embodiment, the end of the main line 34 downstream of the peristaltic pump 36 is integrally provided with an end cap 331 of the cell culture dish 33.

According to an embodiment of the present invention, a through hole is formed in the end cap 331, an end portion of one end of the main pipeline 34 connected to the end cap 331 is integrally disposed with an edge of the through hole located inside (i.e., a side connected to a bottle opening of the bottle body 332) or outside the end cap 331, and an end surface of the end portion of the main pipeline 34 does not extend beyond an inner side surface of the end cap 331.

Through the arrangement, the cell culture dish realizes the airtight construction of the cell culture dish unit by combining the structures of the feeding unit 31, the collecting unit 32, the cell culture dish 33, the pipeline and the like, so that the whole cell culture process can be effectively realized as long as the cleanliness of the whole culture system is ensured, no extra manual work is needed, and the risk that the culture dish is polluted due to manual work is effectively avoided. In addition, the structure of the invention also effectively realizes the automation in the cell culture process, and further effectively improves the culture efficiency.

Through the arrangement, the cover body and the pipeline of the cell culture dish are directly and integrally arranged, so that the problem that the sealing property between the pipeline and the cover body is difficult to solve during system assembly is effectively solved, and the risk that the joint position of the pipeline and the cover body is easily corroded by pollutants is also effectively avoided. In addition, the structure that the pipeline and the cover body are integrally arranged further facilitates the connection with the bottle body of the culture dish, effectively eliminates the contact to the inside of the culture dish in the connection process, greatly ensures the cleanliness of the whole system and is beneficial to ensuring the normal operation of the whole period of the cell culture process.

Through the arrangement, the whole sealing performance is excellent, and the culture dish is beneficial to ensuring the stability and cleanness of the inside of the whole culture dish.

Referring to fig. 1, 2, 3 and 4, according to an embodiment of the present invention, the feeding unit 31 includes: a plurality of first containers 311 for storing a nutrient solution, a plurality of second containers 312 for storing enzymes, a first branch pipe 313 connected to the first containers 311, a second branch pipe 314 connected to the second containers 312, and branch pipe pressure valves 315 provided at the first branch pipe 313 and the second branch pipe 314, respectively. In the present embodiment, on main pipeline 34, first branch pipe 313 and second branch pipe 314 have a spaced staggered arrangement. In this embodiment, the number of the first container 311 and the second container 312 may be adjusted and matched according to the type of cells to be cultured, so as to meet the culture requirement. In the present embodiment, the type of the enzyme stored in the second container may be set as needed, and for example, the e-enzyme for collection may be selectively stored in the second container.

Through the setting, the accurate control of supplying liquid to each container can be realized through setting up the pipe pressure valve on every branch pipe to make the accurate configuration of the nutrient solution of cell culture in-process, guaranteed the normal clear of whole cultivation process.

Through the setting, arrange on the main pipeline with the mode of staggered arrangement through the branch pipe with different kinds of containers to make the liquid in different kinds of containers add the time through the main pipeline simultaneously and can realize the mixture in the pipeline, and then can effectively guarantee that the distribution of different kinds of liquid when getting into the cell culture dish is more even.

Referring to fig. 1, 2, 3 and 4, according to an embodiment of the present invention, the collecting unit 32 includes: a plurality of collecting containers 321, collecting branch pipes 322 connected to the collecting containers 321, collecting pipes 323 connected to the main pipe 34, and collecting pipe pressure valves 324 provided in the collecting branch pipes 322, respectively. In this embodiment, the collection branch pipe 322 is connected to the collection vessel 321 at one end and to the collection pipe 323 at the other end. In the present embodiment, the collecting branch pipes 322 are arranged at intervals on the collecting pipe 323. In this embodiment, the number of the collection containers 321 can be set according to the requirement, so as to ensure the flexibility of the present invention in practical application.

According to one embodiment of the present invention, the collection container 321 includes a collection container cover and a collection container body 3211 detachably coupled to the collection container cover. In this embodiment, the end of the collection manifold 322 is integral with the collection container lid. In this embodiment, the end of the collection manifold 322 may be disposed to extend into the collection container lid or may be flush with the inside surface of the collection container lid.

In this embodiment, two collection containers are provided, one for collecting the culture medium liquid after the culture in the culture dish and the other for collecting the supernatant in the culture dish.

Referring to fig. 1, 2, 3, 4, and 5, according to an embodiment of the present invention, the cell culture dish 33 further includes: bottle body 332 is removably coupled to end cap 331. In this embodiment, the bottle 332 includes: body portion 3321 and neck portion 3322 provided at one side of body portion 3321. In this embodiment, a bottom portion of one end of body portion 3321 connected to neck portion 3322 is provided with an inclined surface 3321a and the rest is a flat surface 3321 b.

With the above arrangement, the position where the neck portion 3322 is connected to the body portion 3321 is advantageous for smooth feeding of liquid by the inclined bottom portion being formed as an inclined surface, so that the liquid is effectively guided to the rear portion of the body 332, which is advantageous for ensuring the normal growth of cells at the rear portion in the culture dish.

In this embodiment, the edge of the inclined surface 3321a provided on the bottle 332 has a leg 3321 c; the bottom ends of legs 3321c are flush with a plane 3321b at the bottom of body portion 3321.

Through the setting, the smoothness of the bottom of the bottle body 332 is effectively guaranteed, the stable placement of the bottom of the bottle body 332 is facilitated, and the stability of the culture environment is guaranteed.

According to one embodiment of the present invention, the vial 332 portion of the cell culture dish 33 is transparent. In the present embodiment, a convex lens is provided on the inclined surface 3321a of the vial 332. In the present embodiment, the convex lens is provided integrally with the inclined surface 3321 a. Through set up convex lens on the inclined plane, the effectual realization is counted and is observed the quantity of cell on the inclined plane, and the cell growth state of the non-horizontal bottom surface position of judgement that can be accurate has guaranteed the accurate grasp to whole culture dish internal culture environment.

According to one embodiment of the invention, the end cap 31 or the bottle body 32 of the cell culture dish 3 is provided with a medical air-permeable filter membrane for air permeation and liquid blocking. In this embodiment, the medical gas permeable filter membrane can only allow gas to pass through, so as to ensure the exchange between the gas in the culture dish and the gas outside the culture dish. The medical air-permeable filter membrane can prevent other substances such as moisture, bacteria and the like from passing through so as to ensure the maintenance of culture solution in the culture dish and play a role of cleaning air.

As shown in fig. 2, according to one embodiment of the present invention, a light sensing calibration device a is disposed on a sidewall of the bottle body 32. Whether the calibration device that whether cell pollutes in the detection culture dish as the culture process through set up light sense calibration device A on the lateral wall at the culture dish can cooperate the sensor to use, improves the promptness to the internal culture environment detects.

Referring to fig. 1, 2, 6, 7, 8 and 9, according to an embodiment of the present invention, the incubator apparatus 2 includes: a box body 21 with a heating function, a box cover 22, a temperature and humidity adjusting device 23 which is arranged at one side of the box body 21 and is positioned at the outer side of the box body 21, a culture dish supporting table 24 which is arranged in the box body 21 and is used for bearing a cell culture dish 33, a microscope device 25 which is arranged at the bottom of the culture dish supporting table 24, and an illuminating device 26 which is positioned above the culture dish supporting table 24; in the present embodiment, the entire case 21 has a symmetrical rectangular structure. The side of the box body 21 connected with the temperature and humidity adjusting device 23 is regularly provided with meshes 21a penetrating through the side wall. In the present embodiment, the meshes 21a are arranged in a symmetrical array on both sides of a center line perpendicular to the longitudinal direction of the case. In the present embodiment, the mesh 21a is positioned above the dish support base 24. In the present embodiment, the temperature/humidity control device 23 performs gas exchange with the inside of the case 21 through the mesh 21a to assist in temperature/humidity control in the case 21.

In the present embodiment, the box 21 has a heating function, and can heat the internal environment to ensure that the internal environment of the box has a suitable cell culture temperature. And when the temperature in the box body is higher, the temperature in the box body can be adjusted by stopping heating the box body 21 and/or operating the temperature and humidity adjusting device 23, so that the temperature of the environment in the box body is always kept in a reasonable range.

In this embodiment, when the humidity control is required to be performed on the environment inside the box body, the humidification or dehumidification can be performed on the environment inside the box body through the independent operation of the temperature and humidity control device 23, so as to keep the humidity of the environment inside the box body constant.

In this embodiment, the number and the size of the openings of the mesh 21a on the box 21 are matched with the volume of the box, and the circulation of the air in the box 21 by the temperature and humidity adjusting device 23 can be realized through the arranged mesh 21a without generating obvious air flow in the box, especially by arranging the mesh 21a on the upper half part of the side wall of the box 21 (namely, above the culture dish supporting table 24), the flow of the air in the box is effectively eliminated, and the stable exchange of the air is realized.

Through the arrangement, the cell culture box has a simple structure, and the temperature and the humidity can be directly controlled in an auxiliary manner through the mesh holes formed in the box wall by directly using the temperature and humidity adjusting device 23 arranged on one side. In addition, the temperature and humidity adjusting device 23 is directly arranged on the wall of the box body 21, so that the ventilation path is effectively shortened, the ventilation pipeline is eliminated, and the temperature and humidity in the box body 21 are accurately controlled.

Referring to fig. 1, 2, 6, 7, 8 and 9, according to an embodiment of the present invention, one side of the cover 22 is detachably and rotatably connected to an upper end of one side of the case 21 connected to the temperature/humidity adjusting device 23. In the present embodiment, the cover 22 is connected to the case 21 by a detachable pin, and when a structure such as a petri dish is mounted in the case 21, the cover 22 is detached from the case 21, and after the petri dish is mounted, the cover 22 is connected to the case 21 to seal the case 21. In the present embodiment, the illumination device 26 is attached to the inner surface of the case cover 22. Wherein the illumination device 26 is removably connected to the cover 22.

Referring to fig. 6, 7, 8 and 10, according to an embodiment of the present invention, an annular sealing and heat insulating strip 211 is provided on an upper end of the box body 21. In the present embodiment, the cross-sectional shape of the annular sealing and heat insulating strip 211 is a semicircle or a semi-ellipse. By setting the upper side of the annular sealing heat insulating strip 211 to be arc-shaped, the line contact with the box cover can be realized, and the line contact with each position of the box cover can be effectively realized.

Through the arrangement, the annular sealing strip is arranged between the box body 21 and the box cover 22, so that a gap between the upper end surface of the box body and the box cover can be effectively eliminated, and further, the stable maintenance of a constant temperature and humidity environment in the box body is ensured.

In the present embodiment, at least one annular groove 211a coaxial with the annular sealing and heat insulating strip 211 is provided on a side of the annular sealing and heat insulating strip 211 away from the box body 21. In the present embodiment, the annular groove 211a has a rectangular cross section. And a vertical surface is formed on the side surface of the annular groove 211a, so that a sharp corner is formed at the connecting position between the upper side of the annular sealing heat insulation strip and the curved surface, and the position where the annular sealing heat insulation strip is contacted with the box cover 22 can realize multiple sealing effects. In addition, the intensity of the sharp corner edge that the annular groove formed on the sealing strip is less, and then can produce small crooked to the recess inboard in the position that contacts with the case lid, and then can produce inseparabler contact with the case lid, in addition can produce when the edge produces small crooked and can with produce the relative slip of less distance between the case lid, can produce the cleaning action to the position that contacts with the case lid, guarantee the leakproofness of contact position. In addition, a plurality of annular grooves 211a can be arranged in parallel at intervals in the width direction of the annular sealing heat insulation strip 211, so that a plurality of edges with different heights can be formed on the surface of the sealing strip, and a multi-sealing effect is achieved.

In the present embodiment, the side of the annular sealing and heat insulating strip 211 corresponding to the position where the box body 21 and the box cover 22 are rotatably connected is provided with a notch 211 b. In this embodiment, the main pipeline 34 is connected to the end cap 331 through the notch 211b, and the width of the notch 211b and the shape of the notch cross section match the shape of the cross section of the main pipeline 34 passing through.

Through the arrangement, the culture dish pipeline can be installed and pass through the gaps by arranging the gaps 211b on the annular sealing heat insulation strips 211. This allows the perimeter of the culture dish tubing to be sealed by the annular sealing insulating strip 211. Especially, under the condition that the box cover is buckled with the box body, the sealing strip is under the action of extrusion force, so that the end face at the position of the notch 211b is in closer contact with the outer side face of the pipeline, and the sealing strip is favorable for ensuring the air tightness around the pipeline. In addition, the positioning effect on the pipeline can be realized through the notch 211b on the sealing strip, an additional clamping structure is not needed, and the installation of the pipeline in the box body 21 is effectively simplified.

Through the setting, through the mode that sets up breach 211b on annular sealed heat insulating strip 211, can be convenient simple replace the mode of opening on box or case lid, and then can effectively eliminate the drawback that leads to the structure gas tightness to worsen at box or case lid upper shed. In addition, the mode of opening on the sealing ring is simple and convenient, and an additional sealing structure is not required to be added, so that the structure is effectively simplified under the condition of ensuring good air tightness.

Referring to fig. 1, 2, 6, 7 and 8, according to an embodiment of the present invention, the portion of the main pipeline 34 extending into the tank may be directly fixed to the side of the tank 21 where the mesh 21a is provided. In the present embodiment, the main pipeline 34 may be extended to be wound in a coil shape on the side of the case 21 where the mesh 21a is disposed, and the center line of the formed loop structure is perpendicular to the side wall of the case 21 to fix the main pipeline 34.

Through the arrangement, when the part of the main pipeline in the box body can realize the exchange between the air flow output by the temperature and humidity adjusting device and the inside of the box body, the temperature of the liquid in the main pipeline is controlled in advance, so that the temperature of the liquid entering the cell culture dish can be closer to the temperature of the liquid in the culture dish, the stable growth of cells is ensured, and the influence caused by the overlarge temperature difference between the external liquid temperature and the liquid temperature in the culture dish is avoided.

In the present embodiment, the part of the main pipeline 34 extending into the box body can also increase the pipe diameter of the main pipeline 34, and the pipe diameter at the end position connected with the end cover 331 of the cell culture dish is reduced (the length of the part with reduced pipe diameter can be set according to actual requirements), and is properly extended so that the main pipeline 34 can be wound and set in a coil shape in a ring shape at the side of the box body 21 where the mesh 21a is arranged, and the center line of the formed ring structure is perpendicular to the side wall of the box body 21 to fix the main pipeline 34.

Through the setting, the liquid that can further make into in the culture dish can have longer dwell time in the incubator to effectively solve the big problem of liquid difference in temperature, and then beneficial to guaranteeing the normal growth of cell in the culture dish.

Referring to fig. 6, 7 and 8, according to an embodiment of the present invention, the box body 21 is a hollow structure surrounded by multiple layers of plate bodies. In the present embodiment, the case 21 includes: the heat-conducting layer, the zone of heating, the heat preservation, the insulating layer that set gradually from inside to outside. In the present embodiment, the heat conductive layer is made of a stainless steel metal plate. The zone of heating is used for heating the heat-conducting layer, and the heat-conducting layer of being made by the metal is convenient for transmit the produced heat of zone of heating to the inside of box 21, and then carries out thermostatic control to the box is inside. In the embodiment, the heating layer is coated on the outer side of the heat conduction layer, the heat preservation layer is coated on the outer side of the heating layer, and the insulation layer is coated on the outer side of the heating layer; the heating layer effectively inhibits the dissipation of heat generated by the heating device, and the constant temperature effect of the accurate control box body is beneficial. In addition, the use safety of the box body 21 is effectively guaranteed through the arranged insulating layer.

In the present embodiment, the position on the heat conduction layer where the mesh 21a is arranged is covered with a sterilization air-permeable film; the isolation of the inside of the box body and the external environment can be realized through the degerming air film arranged, the purification effect on the gas is realized when the gas passes through the degerming air film, and the gas exchange generated gas flow is restrained.

The box body is of a multilayer composite structure, so that the box body is simple in structure and can be conveniently embedded into other systems, and the flexibility of use and installation of the box body is guaranteed. In addition, the box body is provided with the heating layer, so that the direct heating in the box body can be conveniently realized, a middle heat exchange path is eliminated, the contact with the outside is effectively reduced, and the accurate control of the temperature in the box body is facilitated. Meanwhile, because the temperature and humidity adjusting device 23 is directly connected with the box body, the influence of heat generated in the working process of the temperature and humidity adjusting device 23 on the internal environment of the box body can be effectively eliminated by arranging the heat insulating layer and the insulating layer on the box body.

In the present embodiment, case cover 22 is a hollow plate-like body, and its side adjacent to case 21 is made of a heat insulating material. Through the arrangement, the matching with the box body 21 is effectively realized, and the stability of the internal environment of the box body is ensured.

In this embodiment, be provided with carbon dioxide sensor, oxygen sensor, humidity transducer, temperature sensor in box 21 for the internal environment of real-time detection box 21, and then accessible temperature humidity control device 23 and the zone of heating of box realize the real-time accurate adjustment to the internal environment of box, guarantee the equilibrium of temperature and humidity in the box, avoid appearing the gradient difference in the box.

Referring to fig. 7, 8, 9, 11, 12, 13, and 14, according to an embodiment of the present invention, the dish support stand 24 includes: a connecting base 241 supported on the bottom of the case 21, a lifting base 242 connected to the connecting base 241, a driving device 243, and a detecting device 244 for detecting the internal state of the culture dish mounted on the lifting base 242. In the present embodiment, the driving device 243 is supported by the connecting base 241. One end of the lifting seat 242 is a rotation connection end rotatably connected to the connection seat 241, and the other end thereof is a lifting connection end connected to the driving device 243. In this embodiment, the lifting connection end can move up and down relative to the connection base 241 under the driving action of the driving device 243.

Through the arrangement, the lifting seat 242 is used for bearing a culture dish (for example, an adherent cell culture dish), so that the lifting connecting end of the lifting seat can be rotated to vertically lift through the action of the driving device, the lifting seat 242 generates an inclined motion relative to the connecting seat 241, liquid in the culture dish can be enabled to smoothly flow out when the culture dish needs to be replaced, and residues in the culture dish are avoided.

Through the arrangement, the lifting operation of the lifting seat can be automatically realized, manual participation is not required, and the updating efficiency of the liquid in the culture dish can be effectively improved.

Referring to fig. 7, 8, 9, 11, 12, 13 and 14, according to an embodiment of the present invention, the connecting seat 241 includes: a connection frame 2411 and a bottom support 2412 provided on the lower side of the connection frame 2411. In this embodiment, the connection frame 2411 is a continuous rectangular frame, and the size of the frame can be adjusted adaptively according to the size of the inside of the housing. In this embodiment, the bottom support 2412 is a plate-like body and is fixedly attached to the lower side of the connection frame 2411. In the present embodiment, the bottom support 2412 and the connection frame 2411 may be integrally provided, or may be fixed by means of adhesion, screwing, or the like.

In the present embodiment, a plurality of legs are provided at intervals on the lower side of the connection frame 2411. The height of the supporting feet can be adjusted according to the requirement. In the present embodiment, the connection frame 2411 is attached to the bottom of the case 21 by legs. In the present embodiment, the support legs are fixed to the bottom of the case 21 by a connector, so that the petri dish support stand 24 is fixedly attached.

Referring to fig. 7, 8, 9, 11, 12, 13, 14, and 15, according to an embodiment of the present invention, the lifting base 242 is located inside the connecting frame 2411 and above the bottom support 2412, and in this embodiment, the rotating shafts 242a for rotatably connecting with the connecting frame 2411 are respectively provided at opposite sides of the rotating end of the lifting base 242. The rotation shaft 242a is connected to the lifting base 242 and the connection frame 2411 via a bearing, so that the lifting base 242 can rotate flexibly relative to the connection frame 2411. In the present embodiment, in order to connect the lifting base 242 inside the connection frame 2411, the outer shape of the lifting base 242 is matched with the inner shape of the connection frame 2411, and if the inner side of the connection frame 2411 is rectangular, the outer shape of the lifting base 242 should be rectangular.

Referring to fig. 11, 12, 13 and 15, according to an embodiment of the present invention, the lifting base 242 has a frame structure with a dish mounting position 242b for mounting the cell culture dish 33. In this embodiment, the lifting seat 242 is a hollow frame structure, and the dish mounting position 242b for the culture dish thereon is formed by the hollow structure of the lifting seat 242. Specifically, the vessel mounting station 242b includes: a first hollow portion 242b1 and a second hollow portion 242b2 communicating with each other. In the present embodiment, the second hollow portion 242b2 and the first hollow portion 242b1 are sequentially disposed in a direction from the rotation connection end of the lifting and lowering base 242 to the lifting and lowering connection end (i.e., the length direction), wherein the second hollow portion 242b2 has an opening at the end of the rotation connection end of the lifting and lowering base 242. In this embodiment, the widths of the first hollow portion 242b1 and the second hollow portion 242b2 are different, so that a step structure is formed on the vessel mounting position 242b, which is further beneficial to the mounting and positioning of the culture vessel in the first hollow portion 242b1, and the mounting stability of the culture vessel is ensured, and the second hollow portion 242b2 corresponds to the bottle neck and the bottle cap on the culture vessel, and the like, so that the whole culture vessel is stably mounted.

In this embodiment, the second hollow portion 242b2 is open at the end of the rotary connection end to further avoid interference with the structure of the neck portion 3322 and the end cap 331 of the cell culture dish 33, to accommodate the installation of neck portions 3322 and end caps 331 of different sizes, and to provide sufficient space for avoiding interference of the entire dish support table 24 with the culture dish during the lifting and lowering of the lifting and lowering base 242.

Through the above arrangement, the vessel mounting position 242b is arranged to be of a hollow structure, so that the interference position between the vessel mounting position and the culture dish can be effectively reduced, and the stable mounting of the culture dish is facilitated. In addition, the vessel mounting position 242b is hollow, so that the quality of the lifting seat can be greatly reduced, the structure of the lifting seat is simplified, and the long-time stable operation of the lifting seat is ensured. In addition, the hollow vessel mounting position 242b is also beneficial to mounting other optical equipment on the culture vessel supporting table 24 of the invention for observing the state in the culture vessel in real time, thereby effectively improving the use flexibility of the invention.

In this embodiment, the lower side of the end of the rotation connection end of the lifting seat 242 is provided with an arc surface for connecting the end surface and the lower side of the rotation connection end, thereby forming a transition portion at the end position. The arc surface is arranged at the lower side of the end part of the rotating connecting end of the lifting seat 242, which is beneficial to ensuring the smooth rotation of the lifting seat 242 and further ensuring the use stability of the invention.

As shown in fig. 11, 12, 13, 14 and 15, in accordance with one embodiment of the present invention, the lifting seat 242 is provided with light transmission channels 2421 penetrating through at two opposite sides of the vessel mounting position 242b in the width direction of the lifting seat 242; in the present embodiment, the light-transmitting passage 2421 is provided at a position where the first hollow part 242b1 is close to the elevating connection end of the elevating base 242. In the embodiment, the light-transmitting channels 2421 are coaxially arranged on the frame body of the lifting seat 242, and further, when the culture dish is fixed, the light-transmitting channels 2421 can be coaxially arranged on two opposite sides of the culture dish, and further, the light-transmitting channels 2421 on one side are used for polishing, and can pass through the culture dish and then reach the light-transmitting channels 2421 on the other side and be transmitted out, so that the internal state (such as internal environment, cell growth state, pollution condition, nutrient solution turbidity degree and the like) of the culture dish can be collected, and the subsequent detection and analysis can be facilitated. .

In the present embodiment, the light-transmitting passage 2421 is disposed near the lower side of the elevating base 242 in the thickness direction of the elevating base 242, i.e., the light-transmitting passage 2421 is spaced from the lower side by a distance smaller than the distance separating the connecting passage from the upper side.

Through the above arrangement, the light transmission channel 2421 is arranged at the position close to the lifting connection end of the lifting seat 242, so that the internal environment at the rear part of the culture dish can be detected in real time, and accurate detection of the internal environment of the culture dish can be mastered.

Referring to fig. 13 and 15, according to an embodiment of the present invention, a support structure 2422 is disposed at the bottom of the lifting seat 242. In the present embodiment, the support structure 2422 includes: a first support part 2422a and a second support part 2422 b. The first support part 2422a is provided along the inside edge of the bottom of the first hollow part 242b 1; the second support part 2422b is connected to part of the inside edges of the opposite sides of the bottom of the second hollow part 242b 2. In the present embodiment, the first support part 2422a has a long bar-shaped structure, which is continuously or intermittently provided at the inner side edge of the bottom of the first hollow part 242b 1. In the present embodiment, the second support part 2422b is a plate-shaped body, and opposite ends thereof are fixedly connected to inner side edges of portions of the second hollow part 242b2 at opposite sides of the bottom thereof. Effective support for the culture dish edge and the bottleneck portion is provided by providing a support structure 2422 at the bottom edge of the dish mounting station 242 b.

In the present embodiment, the second support part 2422b is provided with a space from the end opening of the rotation connection end. The space between the second support part 2422b and the end opening of the rotary connecting end can play a role of avoiding, so that interference with other structures is avoided, and the normal and stable operation of the invention is ensured.

As shown in fig. 11, 13 and 14, according to an embodiment of the present invention, the driving device 243 includes: a driver 2431 for outputting a linear displacement, and a yoke 2432 connected to the driver 2431. In the present embodiment, the yoke 2432 includes: a driver connecting arm 2432a and a lifting base connecting arm 2432 b; the lifting base connecting arm 2432b is disposed perpendicular to the driver connecting arm 2432 a. In this embodiment, the actuator connecting arm 2432a and the lifter base connecting arm 2432b may be integrally provided, or may be connected by a screw, an adhesive, a caulking, or the like. In this embodiment, two lifting base connecting arms 2432b are provided, which are connected in parallel to the driver connecting arm 2432 a.

Referring to fig. 11, 12 and 14, the bottom support 2412 is provided with a linear channel 2412a according to one embodiment of the present invention. In this embodiment, the end of the lifting base connecting arm 2432b remote from the driver connecting arm 2432a is hinged to the lifting connecting end of the lifting base 242 through the linear passage 2412 a. In this embodiment, a hinge seat for connecting with the driver connecting arm 2432a is provided at the lower side of the lifting connecting end, the hinge seat is formed by two plate-shaped bodies arranged in parallel and at an interval, and the connection can be completed by sequentially passing through the hinge seat and the corresponding mounting positions on the driver connecting arm 2432a through the hinge shaft.

In this embodiment, the lifting base connecting arm 2432b is located on the lower side of the bottom support 2412, and the side away from the driver connecting arm 2432a is hinged to the movable end of the driver 2431. In this embodiment, a first notch is formed on the bottom support 2412 below the rotating connection end of the lifting seat 242, a second notch is formed at a position of the connection frame 2411 of the connection seat 241 corresponding to the first notch, the second notch is communicated with the first notch, and the driving device 243 can be installed at the positions of the first notch and the second notch. In this embodiment, the driver 2431 is a linear driving device, and the telescopic end thereof can move telescopically along a linear direction, so that the free end of the driver 2431 is hinged to the driver connecting arm 2432a to realize the pushing action on the lifting seat 242.

According to an embodiment of the present invention, the driver 2431 is implemented by an electric cylinder, which can precisely control the displacement of the telescopic end thereof through an electric signal, and can automatically and precisely control the inclination angle of the lifting seat through the control of the electric signal, so as to achieve the micro-flow of the nutrient solution in the culture dish and the flexible adjustment of the horizontal position of the nutrient solution. The driver connecting arm 2432a is disposed at the lower side of the bottom support 2412, and the lifting seat 242 is disposed at the upper side of the bottom support 2412, so that in the process of driving the lifting seat 242 to move up and down by connecting the driver 2431 and the driver connecting arm 2432a, the bottom support 2412 can support and limit the driver connecting arm 2432a, thereby facilitating the stable movement of the driver 2431. In addition, through the above arrangement, the accurate control of the inclination angle of the lifting seat 242 can be flexibly realized by controlling the telescopic length of the free end of the driver 2431 without other complex structures, and the advantages of high operation precision, good stability, simple structure and the like are realized.

In the present embodiment, the hinge position of the driver connecting arm 2432a and the driver 2431 is provided with a rubber damper structure for silent rotation of the hinge position. In this embodiment, the accessible sets up the damping structure that rubber was done on articulated position's both sides or articulated shaft to the realization is to articulated position's amortization, but also the effectual smooth and easy stability of having guaranteed the operation and be useful to guaranteeing the accuracy of operating position.

In the present embodiment, the driver connecting arm 2432a is embedded with a position sensor for detecting the movement position thereof. The position of the driver connecting arm 2432a can be accurately controlled by detecting the position of the driver connecting arm 2432a in real time through the position sensor, so that the inclination angle of the lifting seat can be accurately controlled.

In the present embodiment, a limit structure for limiting the maximum displacement of the driver connecting arm 2432a is further provided on the driver connecting arm 2432 a. Excessive movement of the driver connecting arm 2432a can be effectively avoided by changing the limiting structure, so that the maximum rising height of the lifting seat 242 can be effectively restrained, and further, stable operation of the lifting seat is guaranteed, and interference with other structures is eliminated.

As shown in fig. 11 and 12, according to an embodiment of the present invention, a detection device 244 for detecting an internal state of the culture dish mounted on the lifting base 242 is provided on the connection frame 2411. In the present embodiment, the detection device is a correlation detection device. Specifically, the detecting device 244 includes: a laser light source 2441, an optical fiber 2442, and an optical receiver 2443. In this embodiment, mounting through holes penetrating the housing are provided on both opposite sides of the connection housing 2411, respectively, and the laser light source 2441 and the optical receiver 2443 can be mounted in the mounting through holes so as to face each other. The optical fibers 2442 are respectively embedded in the light-transmitting channels 2421 of the lifting seat 242; when the lifting seat 242 is horizontally located in the connecting frame 2411, the laser light source 2441, the optical fiber 2442 and the optical receiver 2443 are aligned, light emitted by the laser light source 2441 can be transmitted to the optical receiver 2443 through the optical fiber 2442, so that the environment in the culture dish can be detected, and when the lifting seat 242 is inclined, the lifting connecting end leaves the connecting frame 2411, so that the detection device 244 can output a lifting signal of the lifting seat 242, so that the environment in the culture dish cannot be detected. In this embodiment, the optical receiver 2443 can process and analyze the received signal by itself, or transmit the signal to other devices for processing and analysis, and the processing and analysis can be set according to practical applications.

Through the arrangement, the automatic detection of the interior of the culture dish can be realized by adopting the detection device to detect the culture dish. In addition, automatic control of the lifting seat can be realized through on-off of the detection device, and the control accuracy of the lifting seat is improved.

In addition, the optical fiber 2442 can be abutted against the culture dish according to the requirement, so that a certain pre-tightening effect can be provided for the culture dish, the reliable fixation of the culture dish is beneficial, the arrangement mode is more beneficial to the condition that the lifting seat is in an inclined state, the problem that the side of the culture dish is turned over due to the accumulation of liquid in the culture dish at the bottleneck end can be effectively avoided through the abutting pre-tightening effect on the rear part of the culture dish, and the operation of the culture dish side-turning device is more reliable. In addition, the optical fiber 2442 and the culture dish can be integrally arranged to further play a role in fixedly connecting the culture dish while eliminating the influence of the contact position on the detection result.

As shown in fig. 11, 12, 13 and 14, according to an embodiment of the present invention, a first opening penetrating the bottom support 2412 is provided on the bottom support 2412, and in this embodiment, the first opening is positioned below the first hollow portion 242b 1. In this embodiment, the size of the first opening can be adjusted as needed to accommodate installation of different structures.

Referring to fig. 9, 11 and 14, according to an embodiment of the present invention, the microscope device 25 includes: a focusing unit 251, an optical lens mounted on the focusing unit 251, and a microscopic imaging unit 252 for receiving an image in the optical lens. In the present embodiment, the focusing unit 251 is mounted on the bottom support 2412 of the joint base 241, and the microscopic imaging unit 252 is located below the bottom support 2412. In the present embodiment, the focusing unit 251 is located at a lower side of the bottom support 2412, and includes: a lens mount 2511, a gear set 2512 for adjusting the vertical position of the lens mount 2511, and a gear drive 2513 for driving the gear set 2512 to rotate. In the present embodiment, the lens mount 2511 is located at the position of the first opening of the bottom support 2412, which performs focus imaging of the transparent culture dish internal state through the installed optical lens for transmission to the microscopic imaging unit 252.

Referring to fig. 9, 11 and 14, according to an embodiment of the present invention, the microscopic imaging unit 252 is installed at the bottom of the box 21, and the microscopic imaging unit 252 is located outside the box 21. In the present embodiment, a second opening is provided at the bottom of the case 21 for transmission of the image in the optical lens to the microscopic imaging unit 252. In the present embodiment, the position where the microscopic imaging unit 252 is connected to the second opening needs to be hermetically connected.

In the present embodiment, the gear drive 2513 of the focus unit 251 is mounted on the bottom of the casing 21, and the gear drive 2513 is located outside the casing 21.

In this embodiment, the focusing unit 251 has a compact structure and high focusing precision by the focusing method of the gear set 512, while the micro-imaging unit 52 has a compact micro-imaging structure, has a small volume, has little influence on the space required for installing the whole cell incubator, ensures the installation flexibility of the cell incubator of the present invention,

according to one embodiment of the invention, the microscopy apparatus 25 further comprises: a first planar displacement structure. In the present embodiment, the focusing unit 251 is connected below the bottom support 2412 by a first plane displacement structure; the first plane displacement structure is used to drive the focusing unit 251 to linearly reciprocate in the horizontal plane. In the present embodiment, the first planar displacement structure includes: the focusing unit is mounted on the first Y-axis drive, and the reciprocating movement of the focusing unit in the horizontal direction is realized through the combined action of the first X-axis drive and the first Y-axis drive.

In this embodiment, to ensure the movement range of the focusing unit, the size of the first opening may be set to a larger opening area according to the movement range, so as to ensure that the focusing unit can detect the focus at each position in the culture dish.

In the present embodiment, in order to ensure that the focusing unit is stably and effectively input to the microscopic imaging unit 252, the microscopic device 25 further includes: a prismatic structure for transferring the image. In the present embodiment, the prism structure includes: a first prism supported on the first planar displacement structure, a second prism supported at the second opening position, and an intermediate prism group for transferring the image in the first prism to the second prism. The prism structure arranged as described above enables an image formed by the optical lens in the focusing unit 251 to be stably input to the microscopic imaging unit 252.

In the present embodiment, the lighting device 26 is provided with a second planar displacement structure for driving the lighting device 26 to linearly reciprocate in a horizontal plane. In the present embodiment, the second planar displacement structure includes: and the focusing unit is arranged on the second Y-axis drive, and the reciprocating movement of the focusing unit in the horizontal direction is realized through the combined action of the second X-axis drive and the second Y-axis drive.

In the present embodiment, the first planar displacement structure and the second planar displacement structure are interlocked.

Through the setting, through setting up lighting device and focusing unit into the linkage, effectual realized the clear formation of image to each part of culture dish, be favorable to comprehensive and clear cell growth state in acquireing the culture dish, guaranteed the comprehensive accurate grasp of the information of whole cell growth cycle to can further provide relevant information to processing system, realize the accurate adjustment to nutrient solution etc. in the culture dish.

As shown in fig. 3, according to an embodiment of the present invention, the interface unit 6 includes: the device comprises a power supply interface 61 for connecting a power supply, an oxygen interface 62 for connecting an external oxygen source, a carbon dioxide interface 63 for connecting an external carbon dioxide source, a nitrogen interface 64 for connecting an external nitrogen source, a safety structure 65 and a switch structure 66. In the embodiment, the input of an external air source and a power supply is realized through the interface unit so as to ensure the normal operation of the whole system. In this embodiment, the gas source interfaces (i.e. the oxygen interface 62, the carbon dioxide interface 63, and the nitrogen interface 64) are connected to the connectors of the incubator device 2, and are respectively controlled by the control unit to open and close, so as to control the input gas during the cell culture process.

As shown in FIG. 1, according to one embodiment of the invention, a container carrier table 5 is arranged on a support table 1 alongside the incubator assembly 2. In the present embodiment, the container support platform 5 is an open platform for supporting the collection containers 321 of the collection unit 32. When the collection container 321 has a plurality, it is disposed side by side on the container stand 5.

According to one embodiment of the invention, the container support platform 5 is rotatably connected to the support platform 1 at one end and is connected to a lifting mechanism for driving the lifting mechanism to lift and lower. In this embodiment, the lifting structure is connected with the control unit, and the lifting height of the lifting structure can be controlled by the control unit, so that the control of the inclination angle of the container bearing platform 5 is realized, the collection container 321 can more easily receive the input liquid, the smooth inflow and inflow speed of the liquid are ensured, and the liquid input efficiency is effectively improved.

According to an embodiment of the present invention, the temperature/humidity adjustment device 23 includes: an air conditioning unit and an air purification unit. In this embodiment, carry out the auxiliary control through the humiture of air conditioning unit to box 21 internal environment, further disinfect and purify the box internal environment through the air purification unit to further guarantee the cleanness of whole box environment.

The foregoing is merely exemplary of particular aspects of the present invention and devices and structures not specifically described herein are understood to be those of ordinary skill in the art and are intended to be implemented in such conventional ways.

The above description is only one embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An adherent cell culture system, comprising: a support table (1), a incubator device (2) embedded in the support table (1), a culture dish device (3), a touch display device (4), a control unit, a container carrying table (5) and an interface unit (6) supported on the support table (1);

the culture dish arrangement (3) comprises: a feeding unit (31), a collecting unit (32), a cell culture dish (33), a main pipeline (34), a first pipe pressure valve (35) and a peristaltic pump (36);

the cell culture dish (33) is located in the incubator device (2);

the collecting unit (32) is connected with the container bearing platform (5);

the first pipe pressure valve (35) and the peristaltic pump (36) are arranged on the main pipeline (34) at intervals;

the feeding unit (31) is connected with the main pipeline (34), and the connection position of the feeding unit (31) and the main pipeline (34) is positioned at the upstream of the first pipe pressure valve (35);

the collecting unit (32) is connected with the main pipeline (34), and the connecting position of the collecting unit (32) and the main pipeline (34) is positioned between the first pipe pressure valve (35) and the peristaltic pump (36);

the end part of the main pipeline (34) positioned at the downstream of the peristaltic pump (36) penetrates through the incubator device (2) and is integrally arranged with an end cover (331) of the cell culture dish (33), and the end surface of the end part does not exceed the inner side surface of the end cover (331).

2. The adherent cell culture system according to claim 1, wherein the feeder unit (31) comprises: a plurality of first containers (311) for storing a nutrient solution, a plurality of second containers (312) for storing enzymes, a first branch pipe (313) connected to the first containers (311), a second branch pipe (314) connected to the second containers (312), and branch pipe pressure valves (315) respectively provided on the first branch pipe (313) and the second branch pipe (314);

-on said main circuit (34), said first branch (313) and said second branch (314) have a spaced staggered arrangement;

the collection unit (32) comprises: a plurality of collecting containers (321), collecting branch pipes (322) connected with the collecting containers (321), collecting pipes (323) connected with the main pipeline (34), and collecting pipe pressure valves (324) respectively arranged on the collecting branch pipes (322);

one end of the collecting branch pipe (322) is connected with the collecting container (321), and the other end of the collecting branch pipe is connected with the collecting pipe (323);

the collection container (321) comprises a collection container cover and a collection container body (3211) detachably connected with the collection container cover;

the end of the collection manifold (322) is integral with the collection container lid.

3. The adherent cell culture system of claim 2, wherein the cell culture dish (33) further comprises: a bottle body (332) detachably connected to the end cap (331);

the bottle body (332) includes: a body portion (3321) and a neck portion (3322) provided at one side of the body portion (3321);

the bottom part of one end of the body part (3321) connected to the neck part (3322) is formed as an inclined surface (3321a), and the rest is a flat surface (3321 b);

the edge of the inclined surface (3321a) is provided with a support leg (3321 c);

the bottom ends of the supporting legs (3321c) are flush with the plane (3321b) of the bottom of the body portion (3321);

the end cover (331) or the bottle body (332) is provided with a medical air-permeable filter membrane for air permeation and liquid resistance.

4. Adherent cell culture system according to claim 3, wherein the incubator device (2) comprises: the cell culture dish heating device comprises a box body (21) with a heating function, a box cover (22), a temperature and humidity adjusting device (23) arranged on one side of the box body (21) and located on the outer side of the box body (21), a culture dish supporting table (24) arranged in the box body (21) and used for bearing a cell culture dish (33), a microscope device (25) arranged at the bottom of the culture dish supporting table (24), and an illuminating device (26) located above the culture dish supporting table (24);

meshes (21a) penetrating through the side wall of the box body (21) are regularly arranged on one side of the box body (21) connected with the temperature and humidity adjusting device (23);

the mesh (21a) is positioned above the culture dish support table (24);

the temperature and humidity adjusting device (23) is in gas exchange with the inside of the box body (21) through the mesh (21a) and is used for assisting in adjusting the temperature and humidity in the box body (21).

5. The adherent cell culture system of claim 4, wherein an upper end of the box (21) is provided with an annular sealing and insulating strip (211);

the cross section of the annular sealing heat insulation strip (211) is semicircular or semielliptical;

at least one annular groove (211a) which is coaxial with the annular sealing heat-insulating strip (211) is arranged on one side, away from the box body (21), of the annular sealing heat-insulating strip (211);

a notch (211b) is formed in the side edge of the annular sealing heat insulation strip (211) corresponding to the position where the box body (21) and the box cover (22) are rotatably connected;

the main pipeline (34) is connected with the end cover (331) through the notch (211 b).

6. The adherent cell culture system of claim 5, wherein the culture dish support platform (24) comprises: a connecting seat (241) supported at the bottom of the box body (21), a lifting seat (242) connected with the connecting seat (241), a driving device (243) and a detecting device (244) for detecting the internal state of the cell culture dish (33);

the drive device (243) is supported on the connecting base (241);

one end of the lifting seat (242) is a rotary connecting end which is rotatably connected with the connecting seat (241), and the other end of the lifting seat is a lifting connecting end which is connected with the driving device (243);

under the driving action of the driving device (243), the lifting connecting end can do lifting motion relative to the connecting seat (241).

7. An adherent cell culture system according to claim 6, wherein the connecting receptacle (241) comprises: a connecting frame body (2411) and a bottom support (2412) arranged on the lower side of the connecting frame body (2411);

the lifting seat (242) is positioned on the inner side of the connecting frame body (2411) and above the bottom support (2412);

the lifting seat (242) is of a frame structure, and a vessel mounting position (242b) for mounting the cell culture vessel (33) is arranged on the lifting seat;

the vessel mounting station (242b) comprises: a first hollow portion (242b1) and a second hollow portion (242b2) communicating with each other;

the second hollow portion (242b2) having an opening at the end of the rotating connection end of the lifting seat (242);

in the width direction of the lifting seat (242), light-transmitting channels (2421) which penetrate through the lifting seat (242) are arranged on two opposite sides of the vessel mounting position (242 b);

the light-transmitting passage (2421) is arranged at a position where the first hollow part (242b1) is close to the lifting connection end of the lifting base (242).

8. The adherent cell culture system of claim 7, wherein the drive device (243) comprises: a driver (2431) for outputting a linear displacement, a yoke (2432) connected to the driver (2431);

the yoke (2432) comprises: a driver connecting arm (2432a) and a lifting base connecting arm (2432 b);

the lifting seat connecting arm (2432b) is arranged perpendicular to the driver connecting arm (2432 a);

a linear channel (2412a) is arranged on the bottom support (2412);

the end of the lifting base connecting arm (2432b) far away from the driver connecting arm (2432a) passes through the linear channel (2412a) and is hinged with the lifting connecting end of the lifting base (242);

the lifting seat connecting arm (2432b) is positioned at the lower side of the bottom support (2412), and one side far away from the driver connecting arm (2432a) is hinged with the movable end of the driver (2431);

the hinging position of the driver connecting arm (2432a) and the driver (2431) is provided with a rubber damping structure for silent rotation of the hinging position;

the driver connecting arm (2432a) is embedded with a position sensor for detecting the moving position thereof, and a limit structure for limiting the maximum displacement of the driver connecting arm (2432 a).

9. Adherent cell culture system according to claim 8, wherein the microscopic means (25) comprise: a focusing unit (251), an optical lens mounted on the focusing unit (251), a microscopic imaging unit (252) for receiving an image in the optical lens;

the focusing unit (251) is mounted on a bottom support (2412) of the connecting base (241);

the microscopic imaging unit (252) is arranged at the bottom of the box body (21), and the microscopic imaging unit (252) is positioned at the outer side of the box body (21);

a gear drive (2513) of the focusing unit (251) is mounted at the bottom of the box body (21), and the gear drive (2513) is positioned at the outer side of the box body (21).

10. Adherent cell culture system according to any of claims 1 to 9, wherein the interface unit (6) comprises: the device comprises a power supply interface (61) for connecting a power supply, an oxygen interface (62) for connecting an external oxygen source, a carbon dioxide interface (63) for connecting an external carbon dioxide source, a nitrogen interface (64) for connecting an external nitrogen source, a safety structure (65) and a switch structure (66);

the control unit is respectively connected with the incubator device (2), the culture dish device (3), the touch display device (4) and the interface unit (6).