CN114085759A - A device that is used for tumour tissue separation of organoid to cultivate to retrieve - Google Patents
A device that is used for tumour tissue separation of organoid to cultivate to retrieve Download PDFInfo
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- CN114085759A CN114085759A CN202111436268.6A CN202111436268A CN114085759A CN 114085759 A CN114085759 A CN 114085759A CN 202111436268 A CN202111436268 A CN 202111436268A CN 114085759 A CN114085759 A CN 114085759A
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- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 75
- 210000001519 tissue Anatomy 0.000 title claims abstract description 70
- 210000002220 organoid Anatomy 0.000 title claims abstract description 21
- 238000000926 separation method Methods 0.000 title claims description 19
- 238000011010 flushing procedure Methods 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 31
- 239000000243 solution Substances 0.000 claims description 24
- 238000005192 partition Methods 0.000 claims description 20
- 238000003756 stirring Methods 0.000 claims description 20
- 239000002002 slurry Substances 0.000 claims description 11
- 239000002504 physiological saline solution Substances 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 7
- 238000004140 cleaning Methods 0.000 description 7
- 238000012546 transfer Methods 0.000 description 6
- 238000007667 floating Methods 0.000 description 5
- 239000012535 impurity Substances 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 2
- 235000017491 Bambusa tulda Nutrition 0.000 description 2
- 241001330002 Bambuseae Species 0.000 description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 2
- 230000000712 assembly Effects 0.000 description 2
- 238000000429 assembly Methods 0.000 description 2
- 239000011425 bamboo Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 238000013019 agitation Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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Abstract
The invention relates to the technical field of medical instruments, in particular to a device for separating and recovering tumor tissues for organoid culture, which comprises a negative-pressure tractor, a separating tank, a collecting tank and a flushing tank, wherein the collecting tank is connected with the separating tank through a pipeline; medical personnel use the negative pressure tractor to draw tumour tissue to the inside of knockout drum, and the jar that drenches utilizes normal saline to drench tumour tissue and filter earlier, and the reintroduction culture solution for tumour tissue floats in the culture solution, and the collection tank absorbs the culture solution that contains tumour tissue, accomplishes tumour tissue's collection, and has guaranteed tumour tissue's activity.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a device for separating and recovering tumor tissues for organoid culture.
Background
In recent years, the incidence of tumors is on the rise, and according to statistics, 457 thousands of new tumor patients in China in 2020 face a huge challenge in tumor treatment. Organoid drug sensitive technology is a new technology which is newly developed in recent years and is used for separating and culturing tumor tissues of tumor patients to construct organoids with genetic heterogeneity, and further guiding clinical treatment schemes through drug sensitive analysis on the tumor organoids, so that a plurality of problems always exist in how to separate and store the tumor tissues with tissue activity.
Disclosure of Invention
The invention aims to provide a device for separating and recovering tumor tissues for organoid culture, and aims to solve the technical problems that medical staff need to manually collect and clean the tumor tissues after an operation is completed, the burden of the medical staff is increased, and the subsequent medical research is influenced because the tumor leaves a human body and loses activity.
In order to achieve the above purpose, the present invention provides a device for separating and recovering tumor tissue for organoid culture, which comprises a negative pressure tractor, a separation tank, a collection tank and a flushing tank, wherein the collection tank is connected to the separation tank through a pipeline, the separation tank comprises a first tank body, a partition plate, a rotary filter assembly, a vibration assembly and a first motor, the partition plate is arranged inside the first tank body, the first motor is arranged below the partition plate, the rotary filter assembly is rotatably connected to an output end of the first motor and is positioned above the partition plate, the number of the vibration assemblies is multiple, each vibration assembly is uniformly arranged between the partition plate and the rotary filter assembly at intervals, a water outlet is arranged on an outer side wall of the first tank body, an electromagnetic valve is arranged inside the water outlet, the negative pressure tractor and the flushing tank are both connected with a pipeline at the top end of the first tank body, and the collecting tank is connected with a pipeline on the side wall of the first tank body;
the rotary filter component comprises a rotating rod, a filter plate, two stirring blocks and stirring pieces, wherein the rotating rod is rotatably connected with an output shaft of the first motor and is positioned above the partition plate, the two stirring blocks are fixedly connected with the rotating rod and positioned on two sides of the rotating rod, the filter plate is fixedly connected with the rotating rod and is positioned at the top end of the rotating rod, the stirring pieces are multiple, and each stirring piece is fixedly connected with the filter plate and is positioned above the filter plate;
every it all includes spacing frame, roof, toper piece, movable rod, spring and slurcam to vibrate the subassembly, spacing frame with baffle fixed connection, and be located the top of baffle, the roof with spacing frame fixed connection, and be located the top of spacing frame, the toper piece with spacing frame sliding connection, and be located the inside of spacing frame, the spring set up in the toper piece with between the roof, the movable rod with toper piece fixed connection, the movable rod passes the roof, and with roof sliding connection, the slurcam with movable rod fixed connection, and be located the top of movable rod.
Medical personnel use the negative pressure tractor to draw tumour tissue to the inside of knockout drum, the jar that drenches utilizes normal saline to drench and filter tumour tissue earlier, and the reintroduction culture solution for tumour tissue floats in the culture solution, the collection tank absorbs the culture solution that contains tumour tissue, accomplishes tumour tissue's collection to the activity of tumour tissue has been guaranteed.
Wherein, the negative pressure tractor includes the voltage regulator, attracts pipe, the second jar of body and toper pipe, the second jar of body set up in the top of the first jar of body, attract the pipe with the second jar of body intercommunication, the voltage regulator set up in the top of the second jar of body, the toper pipe with the second jar of body fixed connection, and be located the below of the second jar of body, the toper pipe with the first jar of body intercommunication, the inside of toper pipe is provided with the solenoid valve.
Utilize the pressure regulator adjusts suction, will the mouth of pipe of attracting the pipe aims at the position that needs the absorption, absorbs tumor tissue, and tumor tissue soaks the second jar of body, and after the absorption was accomplished, the solenoid valve of below was opened, and tumor tissue follows the toper pipe drops extremely in the first jar of body.
Wherein, the drench jar includes the third jar of body, trunk line, water pump, switching-over valve, auxiliary pipeline and output tube, the third jar of body set up in one side of the first jar of body, the inside of the third jar of body is provided with first cavity and second cavity respectively, the inside packing of first cavity has normal saline, the inside packing of second cavity has the culture solution, the both ends of trunk line respectively with the switching-over valve with first cavity intercommunication, the both ends of auxiliary pipeline respectively with the second cavity with the switching-over valve intercommunication, the both ends of output tube respectively with the water pump with the top intercommunication of the first jar of body, the water pump with the switching-over valve pipe connection.
After the tumor tissue enters the first tank body, the water pump is started, physiological saline is extracted from the first cavity and sent to the first tank body, the reversing valve switches the passage after the first tank body is stirred and filtered, and the water pump extracts culture solution from the second cavity and sends the culture solution to the first tank body.
The spraying tank also comprises a spraying head which is fixedly connected with the output pipe and is positioned inside the first tank body.
When the normal saline is sent to the first tank body, the tumor tissue above the rotary filtering component is flushed through the flushing head.
The collecting tank comprises a fourth tank body and a slurry pump, and two ends of the slurry pump are respectively connected with the first tank body and the fourth tank body through pipelines.
After the culture solution is sent into the first tank, the tumor tissue is dispersed and floats in the culture solution, the slurry pump is used for pumping, and the culture solution containing the tumor tissue is sent into the fourth tank.
The collecting tank further comprises a transferring cylinder, and the transferring cylinder is arranged inside the fourth tank body.
Through setting up the transcompartment section of thick bamboo, the culture solution that contains tumor tissue sends into the inside of transcompartment section of thick bamboo conveniently carries out subsequent processing.
The invention relates to a device for separating and recovering tumor tissues for organoid culture, wherein medical care personnel uses a negative pressure tractor to draw the tumor tissues to the inside of a separation tank, the tumor tissues are firstly flushed by normal saline in the flushing tank, in the flushing process, a first motor is started to drive a rotary filter component to rotate, the flushing area is enlarged, when the liquid level formed by the normal saline exceeds the tumor tissues on the rotary filter component, a water pump is stopped, the first motor continuously rotates to enable shifting blocks on two sides of a rotating rod to rotate, the shifting blocks sequentially push each conical block, the conical blocks push a pushing plate to move through the rotating rod, due to the arrangement of a spring, the pushing plate can descend to form linear reciprocating motion, a plurality of pushing plates move to form local oscillation in the normal saline, the stirring sheet is used for stirring tumor tissues, so that the tumor tissues are reduced in volume and separated from impurities, and the filter plate is not easy to block; after the filtration and separation are finished, the electromagnetic valve of the water outlet is opened, the physiological saline and the impurities are completely discharged and then closed, the culture solution is introduced through the flushing tank, so that the tumor tissue floats in the culture solution, the culture solution containing the tumor tissue is absorbed by the collecting tank, the collection of the tumor tissue is finished, and the activity of the tumor tissue is ensured.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a perspective view of the device for separating and recovering tumor tissue for organoid culture according to the present invention.
Fig. 2 is an enlarged view of a portion of the structure at a provided in the present invention.
Fig. 3 is a sectional view of a separator tank provided by the present invention.
Fig. 4 is a cross-sectional view of an oscillating assembly according to the present invention.
Fig. 5 is a cross-sectional view of a collection canister provided by the present invention.
1-negative pressure tractor, 2-separation tank, 3-collection tank, 4-showering tank, 5-first tank, 6-partition plate, 7-rotary filtering component, 8-oscillating component, 9-first motor, 10-rotary rod, 11-filter plate, 12-toggle block, 13-agitation piece, 14-limit frame, 15-top plate, 16-conical block, 17-movable rod, 18-spring, 19-push plate, 20-water outlet, 21-pressure regulator, 22-suction tube, 23-second tank, 24-conical tube, 25-third tank, 26-main tube, 27-water pump, 28-reversing valve, 29-auxiliary tube, 30-output tube, 31-first cavity, 32-second cavity, 33-a shower head, 34-a fourth tank body, 35-a slurry pump, 36-a transfer cylinder, 37-a second motor, 38-a blade, 39-a cylinder body, 40-a cover plate, 41-a guide frame, 42-a floating plate, 43-a water inlet hole and 44-a cleaning port.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships illustrated in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, are not to be construed as limiting the present invention. Further, in the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Referring to fig. 1 to 5, the present invention provides a device for separating and recovering tumor tissue for organoid culture, the device capable of separating and recovering tumor tissue for organoid culture comprises a negative pressure tractor 1, a separation tank 2, a collection tank 3 and a flushing tank 4, the collection tank 3 is connected to the separation tank 2 through a pipeline, the separation tank 2 comprises a first tank 5, a partition plate 6, a rotating filter assembly 7, a vibrating assembly 8 and a first motor 9, the partition plate 6 is disposed inside the first tank 5, the first motor 9 is disposed below the partition plate 6, the rotating filter assembly 7 is rotatably connected to an output end of the first motor 9 and is disposed above the partition plate 6, the number of the vibrating assemblies 8 is plural, each vibrating assembly 8 is uniformly disposed between the partition plate 6 and the rotating filter assembly 7, the outer side wall of the first tank body 5 is provided with a water outlet 20, an electromagnetic valve is arranged inside the water outlet 20, the negative pressure tractor 1 and the showering tank 4 are both connected with a top pipeline of the first tank body 5, and the collecting tank 3 is connected with a side wall pipeline of the first tank body 5;
the rotating filter assembly 7 comprises a rotating rod 10, a filter plate 11, two stirring blocks 12 and stirring pieces 13, wherein the rotating rod 10 is rotatably connected with an output shaft of the first motor 9 and is positioned above the partition plate 6, the two stirring blocks 12 are fixedly connected with the rotating rod 10 and are positioned at two sides of the rotating rod 10, the filter plate 11 is fixedly connected with the rotating rod 10 and is positioned at the top end of the rotating rod 10, the stirring pieces 13 are multiple, and each stirring piece 13 is fixedly connected with the filter plate 11 and is positioned above the filter plate 11;
each it all includes spacing frame 14, roof 15, toper piece 16, movable rod 17, spring 18 and catch plate 19 to vibrate subassembly 8, spacing frame 14 with 6 fixed connection of baffle, and be located the top of baffle 6, roof 15 with 14 fixed connection of spacing frame, and be located spacing frame 14's top, toper piece 16 with spacing frame 14 sliding connection, and be located spacing frame 14's inside, spring 18 set up in toper piece 16 with between the roof 15, movable rod 17 with 16 fixed connection of toper piece, movable rod 17 passes roof 15, and with 15 sliding connection of roof, catch plate 19 with 17 fixed connection of movable rod, and be located the top of movable rod 17.
In this embodiment, a medical worker pulls tumor tissue into the separation tank 2 by using the negative pressure tractor 1, the flushing tank 4 firstly utilizes normal saline to flush the tumor tissue, in the flushing process, the first motor 9 is started to drive the rotary filter assembly 7 to rotate, the flushing area is enlarged, when the liquid level formed by the normal saline exceeds the tumor tissue on the rotary filter assembly 7, the water pump 27 is stopped, the first motor 9 continuously rotates to rotate the toggle blocks 12 at the two sides of the rotating rod 10, the toggle blocks 12 sequentially push each conical block 16, the conical blocks 16 push the pushing plates 19 to move through the rotating rod 10, due to the arrangement of the springs 18, the pushing plates 19 can descend to form linear reciprocating motion, and the pushing plates 19 move to form local oscillation inside the normal saline, the stirring sheet 13 is used for stirring tumor tissues, so that the tumor tissues are reduced in volume and separated from impurities, and the filter plate 11 is not easy to block; after the filtration and separation are finished, opening the electromagnetic valve of the water outlet 20, and closing the electromagnetic valve after the physiological saline and the impurities are completely discharged; the culture solution is introduced into the leaching tank 4 again, so that the tumor tissue floats in the culture solution, the collection tank 3 absorbs the culture solution containing the tumor tissue to finish the collection of the tumor tissue and ensure the activity of the tumor tissue; the whole is controlled by a PLC system, and the workload of medical personnel is reduced.
Further, the negative pressure tractor 1 comprises a pressure regulator 21, a suction tube 22, a second tank 23 and a conical tube 24, the second tank 23 is arranged above the first tank 5, the suction tube 22 is communicated with the second tank 23, the pressure regulator 21 is arranged above the second tank 23, the conical tube 24 is fixedly connected with the second tank 23 and is positioned below the second tank 23, the conical tube 24 is communicated with the first tank 5, and an electromagnetic valve is arranged inside the conical tube 24; the sprinkling tank 4 comprises a third tank body 25, a main pipeline 26, a water pump 27, a reversing valve 28, an auxiliary pipeline 29 and an output pipe 30, wherein the third tank body 25 is arranged on one side of the first tank body 5, a first cavity 31 and a second cavity 32 are respectively arranged inside the third tank body 25, physiological saline is filled inside the first cavity 31, a culture solution is filled inside the second cavity 32, two ends of the main pipeline 26 are respectively communicated with the reversing valve 28 and the first cavity 31, two ends of the auxiliary pipeline 29 are respectively communicated with the second cavity 32 and the reversing valve 28, two ends of the output pipe 30 are respectively communicated with the water pump 27 and the top end of the first tank body 5, and the water pump 27 is connected with the reversing valve 28 through a pipeline; the sprinkling tank 4 further comprises a sprinkling head 33, and the sprinkling head 33 is fixedly connected with the output pipe 30 and is positioned inside the first tank body 5.
In this embodiment, the pressure regulator 21 is used to adjust the suction force, the nozzle of the suction tube 22 is aligned to the part to be absorbed, the tumor tissue is immersed in the second tank 23, after the absorption is completed, the electromagnetic valve below is opened, the tumor tissue falls into the first tank 5 along the tapered tube 24, after the tumor tissue enters the first tank 5, the water pump 27 is started to pump the physiological saline from the first cavity 31 and send the physiological saline into the first tank 5, after the first tank 5 is stirred and filtered, the reversing valve 28 switches the passage, and the water pump 27 pumps the culture solution from the second cavity 32 and sends the culture solution into the first tank 5; when the physiological saline is sent to the first tank 5, the tumor tissue above the rotary filter assembly 7 is flushed through the flushing head 33.
Further, the collecting tank 3 comprises a fourth tank 34 and a slurry pump 35, and two ends of the slurry pump 35 are respectively connected with the first tank 5 and the fourth tank 34 through pipelines; the collecting tank 3 further comprises a transfer cylinder 36, and the transfer cylinder 36 is arranged inside the fourth tank 34.
In this embodiment, after the culture solution is fed into the first tank 5, the tumor tissue is dispersed and floated in the culture solution, and the culture solution containing the tumor tissue is pumped by the slurry pump 35 and fed into the inside of the transfer cylinder 36 for the convenience of the subsequent treatment.
Further, separator tank 2 still includes second motor 37 and blade 38, second motor 37 set up in the top of the first jar of body 5 is and is located one side of negative pressure tractor 1, blade 38 with the output of second motor 37 rotates to be connected, and is located the inside of the first jar of body 5, and is located the below of negative pressure tractor 1.
In this embodiment, the tumor tissue follows negative pressure tractor 1 gets into when the first jar of body 5, process blade 38, second motor 37 drives blade 38 rotates for the tumor tissue is broken up, drops to the top of rotating filter component 7 accomplishes preliminary separation, improves subsequent separation effect.
Further, the transfer drum 36 includes a cylindrical body 39, a cover plate 40, a guide frame 41, and a floating plate 42, the cylindrical body 39 is disposed inside the fourth tank 34, a water inlet hole 43 is formed at a top end of the cover plate 40, the cover plate 40 is screwed to a top end of the cylindrical body 39, the guide frame 41 is fixedly connected to the cover plate 40 and is positioned below the water inlet hole 43, and the floating plate 42 is slidably connected to the guide frame 41 and is positioned inside the guide frame 41.
In the present embodiment, the culture medium containing the tumor tissue is sent to the transfer cylinder 36 by the slurry pump 35, enters the inside of the cylindrical body 39 through the water inlet hole 43 above the cover plate 40, and the floating plate 42 continues to rise as the liquid level in the cylindrical body 39 rises until the water inlet hole 43 is closed by the floating plate 42, and the slurry pump 35 stops operating.
Further, a cleaning port 44 is arranged on the side wall of the first tank 5, the cleaning port 44 is located above the partition plate 6, and an electromagnetic valve is arranged inside the cleaning port 44.
In this embodiment, after the separation is completed, the electromagnetic valve in the cleaning port 44 is opened, the water pump 27 continuously pumps the physiological saline, the first motor 9 is started, and the tumor tissue remaining on the filter plate 11 is thrown out from the cleaning port 44, thereby completing the self-cleaning of the filter plate 11.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (7)
1. A device for separating and recovering tumor tissues for organoid culture is characterized in that,
the device for separating and recovering tumor tissues for organoid culture comprises a negative pressure tractor, a separation tank, a collection tank and a flushing tank, wherein the collection tank is connected with the separation tank through a pipeline, the separation tank comprises a first tank body, a partition plate, a rotating filtering component, a vibrating component and a first motor, the partition plate is arranged in the first tank body, the first motor is arranged below the partition plate, the rotating filtering component is rotatably connected with the output end of the first motor and is positioned above the partition plate, the vibrating component is in multiple number, each vibrating component is uniformly arranged between the partition plate and the rotating filtering component at intervals, a water outlet is formed in the outer side wall of the first tank body, an electromagnetic valve is arranged in the water outlet, and the negative pressure tractor and the flushing tank are connected with the top pipeline of the first tank body, the collecting tank is connected with a side wall pipeline of the first tank body;
the rotating filter assembly comprises a rotating rod, a filter plate, a stirring block and a stirring piece, wherein the rotating rod is connected with the output shaft of the first motor in a rotating mode and is located above the partition plate, the number of the stirring block is two, the stirring block is fixedly connected with the rotating rod and is located on two sides of the rotating rod, the filter plate is fixedly connected with the rotating rod and is located on the top end of the rotating rod, the number of the stirring piece is multiple, every, and the stirring piece is fixedly connected with the filter plate and is located above the filter plate.
2. The device for separating and recovering tumor tissue in organoid culture according to claim 1,
every it all includes spacing frame, roof, toper piece, movable rod, spring and slurcam to vibrate the subassembly, spacing frame with baffle fixed connection, and be located the top of baffle, the roof with spacing frame fixed connection, and be located the top of spacing frame, the toper piece with spacing frame sliding connection, and be located the inside of spacing frame, the spring set up in the toper piece with between the roof, the movable rod with toper piece fixed connection, the movable rod passes the roof, and with roof sliding connection, the slurcam with movable rod fixed connection, and be located the top of movable rod.
3. The device for separating and recovering tumor tissue in organoid culture according to claim 2,
the negative pressure tractor includes the voltage regulator, attracts pipe, the second jar of body and toper pipe, the second jar of body set up in the top of the first jar of body, attract the pipe with the second jar of body intercommunication, the voltage regulator set up in the top of the second jar of body, the toper pipe with the second jar of body fixed connection, and be located the below of the second jar of body, the toper pipe with the first jar of body intercommunication, the inside of toper pipe is provided with the solenoid valve.
4. The device for separating and recovering tumor tissue in organoid culture according to claim 3,
the drenching tank comprises a third tank body, a main pipeline, a water pump, a reversing valve, an auxiliary pipeline and an output pipe, wherein the third tank body is arranged on one side of the first tank body, a first cavity and a second cavity are respectively arranged in the third tank body, physiological saline is filled in the first cavity, a culture solution is filled in the second cavity, two ends of the main pipeline are respectively communicated with the reversing valve and the first cavity, two ends of the auxiliary pipeline are respectively communicated with the second cavity and the reversing valve, two ends of the output pipe are respectively communicated with the water pump and the top end of the first tank body, and the water pump is connected with the reversing valve through a pipeline.
5. The device for separating and recovering tumor tissue in organoid culture according to claim 4,
the sprinkling tank also comprises a sprinkling head which is fixedly connected with the output pipe and is positioned inside the first tank body.
6. The device for separating and recovering tumor tissue in organoid culture according to claim 5,
the collecting tank comprises a fourth tank body and a slurry pump, and two ends of the slurry pump are respectively connected with the first tank body and the fourth tank body through pipelines.
7. The device for separating and recovering tumor tissue in organoid culture according to claim 6,
the collecting tank further comprises a transferring cylinder, and the transferring cylinder is arranged inside the fourth tank body.
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| US20090148946A1 (en) * | 2007-12-10 | 2009-06-11 | Ryoichi Haga | Separation system for cell, and cell culture system using separator for cell, and method of separation for cell |
| CN110917418A (en) * | 2019-12-26 | 2020-03-27 | 张洪亮 | Device capable of separating and recovering brain tumor tissues |
| CN211862865U (en) * | 2020-02-21 | 2020-11-06 | 东阳市人民医院 | Tumor pathology specimen collecting device |
| CN112358958A (en) * | 2020-11-23 | 2021-02-12 | 浙江省人民医院 | Sorting and filtering device for tumor cell mass for oncology department |
| CN212703443U (en) * | 2020-05-09 | 2021-03-16 | 贵州医科大学附属医院 | Device for separating and recovering brain tumor tissues |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090148946A1 (en) * | 2007-12-10 | 2009-06-11 | Ryoichi Haga | Separation system for cell, and cell culture system using separator for cell, and method of separation for cell |
| CN110917418A (en) * | 2019-12-26 | 2020-03-27 | 张洪亮 | Device capable of separating and recovering brain tumor tissues |
| CN211862865U (en) * | 2020-02-21 | 2020-11-06 | 东阳市人民医院 | Tumor pathology specimen collecting device |
| CN212703443U (en) * | 2020-05-09 | 2021-03-16 | 贵州医科大学附属医院 | Device for separating and recovering brain tumor tissues |
| CN112358958A (en) * | 2020-11-23 | 2021-02-12 | 浙江省人民医院 | Sorting and filtering device for tumor cell mass for oncology department |
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