CN112358958A

CN112358958A - Sorting and filtering device for tumor cell mass for oncology department

Info

Publication number: CN112358958A
Application number: CN202011322998.9A
Authority: CN
Inventors: 陈晓怡
Original assignee: Zhejiang Provincial Peoples Hospital
Current assignee: Zhejiang Provincial Peoples Hospital
Priority date: 2020-11-23
Filing date: 2020-11-23
Publication date: 2021-02-12
Anticipated expiration: 2040-11-23
Also published as: CN112358958B

Abstract

The invention discloses a sorting and filtering device for tumor cell clusters used in oncology department, and mainly relates to the sorting field of tumor cell clusters. It includes a base, the base is provided with a limit plate, the movable rod is slidably connected with the limit plate, one end of the movable rod is provided with a connecting block, the outer shell of the movable rod is provided with a first spring, and one side of the limit plate is A push block is rotated on the base of the machine, and the push block is in contact with the connection block. The test tube is connected with the test tube, and a filter part is detachably arranged in the test tube. The beneficial effects of the present invention are: shaking the test tube in the process of filtering and sorting the tumor cell mass, improving the efficiency of filtering and sorting, without manual shaking, saving manpower and further improving the sorting efficiency.

Description

Sorting and filtering device for tumor cell mass for oncology department

Technical Field

The invention relates to the field of sorting of tumor cell clusters, in particular to a sorting and filtering device of tumor cell clusters for oncology.

Background

The doctor of oncology often needs to sort tumour cell group in the course of working, has appeared on the market at present a device for sorting cell group, is used for specially sorting tumour cell.

For example, the chinese patent application No. CN201721033350.3 discloses a simple sorting and filtering device for tumor cell mass, which comprises a first filtering member, a second filtering member and a connecting pipe. The bottoms of the two filtering pieces are both filter screens; the top of the connecting pipe is provided with a funnel, the outer wall of the connecting pipe is provided with a pipe nozzle, and the position of the pipe nozzle is not lower than the height 1/3 above the bottom of the funnel. When the device is used, sample liquid sequentially passes through the first filtering piece and the second filtering piece, the first filtering piece filters large impurities such as tissue blocks, tumor cell masses are intercepted by the second filtering piece, and single cells and fine impurity particles pass through the second filtering piece and flow into the connecting pipe to be removed. The device can filter once to obtain cell clusters with uniform size, and can remarkably reduce the cell cluster quantity loss and the cell cluster activity damage caused by repeated operation; and the single cell and the fine impurities can be effectively removed, the interference of background signals generated in the subsequent test due to excessive fine impurity particles is avoided, the cell cluster sorting process is standardized on the whole, and the quality controllability of the test process is improved.

However, the simple sorting and filtering device for tumor cell mass has the following defects: the device is at the sorting process of tumour cell group, only relies on the self gravity of cell group to filter for tumour cell group's filtration is selected separately under the inefficiency, and if relies on the artifical filtration that rocks the test tube and promote cell group to select separately, will increase the workman originally, and wastes time and energy, and efficiency is still not high.

Disclosure of Invention

The invention aims to provide a sorting and filtering device for tumor cell clusters for oncology, which can shake a test tube in the process of filtering and sorting the tumor cell clusters, improves the efficiency of filtering and sorting, does not need manual shaking, saves manpower, and further improves the sorting efficiency.

In order to achieve the purpose, the invention is realized by the following technical scheme:

the sorting and filtering device for the tumor cell clusters for the oncology department comprises a base, wherein a limiting plate is arranged on the base, a movable rod penetrating through the limiting plate is arranged on the limiting plate and is in sliding connection with the limiting plate,

one end of the movable rod is provided with a connecting block, a first spring is sleeved outside the movable rod and is positioned between the connecting block and the limiting plate, a pushing block is rotatably arranged on the base at one side of the limiting plate, the length and the width of the pushing block are different, the outer side of the pushing block is contacted with one end of the connecting block, which is far away from the movable rod, a first motor is arranged on the base and is in transmission connection with the pushing block through a meshed bevel gear,

one end of the movable rod, which is far away from the connecting block, is connected with a test tube through a connecting piece, a filter part is detachably arranged in the test tube, one side of the test tube is communicated with an air outlet pipe,

the filter component comprises a first filter screen and a second filter screen which are arranged in a bowl shape from top to bottom, the aperture of the first filter screen is larger than that of the second filter screen, the outer sides of the first filter screen and the second filter screen are fixedly connected through a connecting rod, and the top end of the connecting rod is detachably connected with the pipe orifice wall of the test tube.

Further, the test tube comprises a tube part and a cup part, and the cup part is in threaded connection with the bottom end of the tube part.

Further, the connecting piece is joint bearing, the test tube bottom is equipped with the traction ring, the traction ring passes through elevating gear and installs on the base, the traction ring passes through elevating gear and can dismantle with the test tube bottom and be connected.

Furthermore, elevating gear includes the screw rod of being connected with the base rotation, traction ring one side is equipped with the connecting plate, the screw rod run through the connecting plate and with connecting plate threaded connection, still be equipped with the gag lever post that runs through the connecting plate on the base of screw rod one side.

Furthermore, draw the intra-annular to be equipped with the rubber circle, rubber circle top is for leaking hopper-shaped that suits with the test tube bottom.

Further, be equipped with the bracing piece on the base, the bracing piece is located between limiting plate and the test tube, the bracing piece top is equipped with the spacing ring, the movable rod run through the spacing ring mesopore and with spacing ring sliding connection.

Furthermore, be equipped with a plurality of arcs in the spacing ring, it is a plurality of become an annular after the arc concatenation, it is a plurality of the annular internal diameter that the arc concatenation becomes is less than the diameter of movable rod, be connected with the second spring between the arc outside and the spacing ring inboard, the surface contact in movable rod surface and the arc.

Furthermore, one side of the arc-shaped plate, which is close to the movable rod, is provided with a ball.

Furthermore, a beating rod is rotatably arranged on the base, the end part of the beating rod can be in contact with the supporting rod, a second motor is arranged on the base, and the second motor is in transmission connection with the beating rod through a meshed bevel gear.

Furthermore, the cross section of the pushing block is rhombic, the included angle of the rhombic pushing block is arc-shaped, and one end, close to the pushing block, of the connecting block is also arc-shaped.

Compared with the prior art, the invention has the beneficial effects that:

1. install the test tube at the tip of movable rod through the connecting piece, first motor drives the ejector pad through the bevel gear of meshing and rotates, pivoted ejector pad will promote the connecting block of the movable rod other end, make the movable rod drive the test tube to the direction motion of keeping away from the ejector pad and extrude first spring, because the length and the width of ejector pad are different, when the width one side of ejector pad contacts with the connecting block, make the movable rod move to the direction that is close to the ejector pad under the effect of first spring, thereby make the test tube be horizontal reciprocating motion under the drive of device whole, play the effect that the level rocked to the test tube, improve the efficiency that cell group filters and selects separately, use manpower sparingly;

2. first filter screen and second filter screen pass through the connecting rod and can dismantle with the test tube and be connected, take out first filter screen and second filter screen through the connecting rod after filtering sorting to the cell group after the convenient screening is collected and is preserved.

Drawings

FIG. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a schematic top view of the push block of the present invention.

Fig. 3 is a schematic top view of the connector block of the present invention.

Fig. 4 is a front structural cross-sectional view of the traction ring of the present invention.

Figure 5 is a side view of a stop collar of the present invention.

Fig. 6 is an enlarged view of the structure of the portion a in fig. 1 of the present invention.

Reference numerals shown in the drawings:

1. a base; 2. a limiting plate; 3. a movable rod; 4. connecting blocks; 5. a first spring; 6. a push block; 7. a first motor; 8. a test tube; 9. an air outlet pipe; 10. a first filter screen; 11. a second filter screen; 12. a connecting rod; 13. a tube portion; 14. a cup portion; 15. a connecting member; 16. a traction ring; 17. a screw; 18. a connecting plate; 19. a limiting rod; 20. a rubber ring; 21. a support bar; 22. a limiting ring; 23. an arc-shaped plate; 24. a second spring; 25. a ball bearing; 26. a striking rod; 27. a second motor.

Detailed Description

The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and these equivalents also fall within the scope of the present application.

The invention relates to a sorting and filtering device for tumor cell clusters for oncology, which comprises a base 1, wherein a vertical limiting plate 2 is arranged on the base 1, a movable rod 3 penetrating through the limiting plate 2 is arranged on the limiting plate 2, the movable rod 3 is connected with the limiting plate 2 in a sliding way,

the device is characterized in that a connecting block 4 is arranged at one end of the movable rod 3, a first spring 5 is sleeved outside the movable rod 3, the first spring 5 is positioned between the connecting block 4 and the limiting plate 2, a push block 6 is arranged on the base 1 on one side of the limiting plate 2 in a rotating manner, the length and the width of the push block 6 are different, the outer side of the push block 6 is in contact with one end of the connecting block 4, which is far away from the movable rod 3, a first motor 7 is arranged on the base 1, the first motor 7 is in transmission connection with the push block 6 through a meshed bevel gear, one end of the movable rod 3, which is far away from the connecting block 4, is connected with a test tube 8 through a connecting piece 15, the connecting piece 15 can be an annular fixed part such as a hoop sleeve, a filtering part is detachably arranged in the test tube 8, an outlet pipe 9 is communicated with one side of the test tube 8, the first motor 7 is a speed reducing, the movable rod 3 is pushed to move in the direction far away from the push block 6, the first spring 5 is extruded, then the push block 6 rotates to the position where the width side edge is contacted with the connecting block 4, the distance between the central shaft of the push block 6 and the outer side of the connecting block 4 is reduced, the movable rod 3 is pushed to the position close to the push block 6 under the action of the first spring 5, and along with the rotation of the push block 6, the movable rod 3 is enabled to do horizontal reciprocating motion regularly, so that the test tube 8 at the end part of the movable rod 3 is shaken, the cell mass filtering and sorting efficiency is improved, and manpower is saved;

the filter part includes first filter screen 10 and the second filter screen 11 of the bowl form that top-down set up, the aperture of first filter screen 10 is greater than the aperture of second filter screen 11, guarantees the filtration separation effect of cell group, connecting rod 12 fixed connection is passed through in the outside of first filter screen 10 and second filter screen 11, the mouth of pipe wall overlap joint of horizontal plate and test tube 8 is passed through on the top of connecting rod 12, or the top of connecting rod 12 is fixed at the mouth of pipe of test tube 8 through an elastic splint centre gripping, realizes connecting rod 12 and orificial dismantlement and is connected, puts into test tube 8 and takes out from test tube 8 with first filter screen 10 and second filter screen 11 are whole through connecting rod 12, conveniently collects the processing to the cell group after the sorting and the great tissue that filters down, and the filter screen of bowl form also is convenient for depositing the dress.

Preferably, the test tube 8 includes pipe portion 13 and cup portion 14, cup portion 14 and the threaded connection of pipe portion 13 bottom, the cell sap or the interstitial fluid that are filtered are collected in cup portion 14, pull down cup portion 14 from pipe portion 13 bottom after sorting and can carry out recovery processing to cell sap or interstitial fluid, need not dismantle whole test tube 8, and under the effect of connecting rod 12, the operation of collecting the cell group after sorting also need not dismantle whole test tube 8, prevent that the test tube 8 from dismantling the in-process and taking place the damage of colliding with.

Preferably, connecting piece 15 is joint bearing, 8 bottoms of test tube are equipped with traction ring 16, traction ring 16 passes through elevating gear and installs on base 1, traction ring 16 passes through elevating gear and can dismantle with 8 bottoms of test tube and be connected, make the fixed cover of traction ring 16 in 8 bottoms of test tube through elevating gear, when ejector pad 6 promotes 3 reciprocating motion of movable rod, traction ring 16 carries out the tractive to 8 bottoms of test tube and fixes, at whole reciprocating motion's in-process, the rigidity in position of 8 bottoms of test tube, make 8 upper portions of test tube become around the state of 8 bottoms of test tube left and right rocking under joint bearing's mating reaction, increase the range that 8 rocks of test tube, further promote cell mass sorting filtration speed, thereby improve sorting efficiency.

Preferably, the lifting device comprises a screw rod 17 rotationally connected with the base 1, a connecting plate 18 is arranged on one side of the traction ring 16, the screw rod 17 penetrates through the connecting plate 18 and is in threaded connection with the connecting plate 18, a limiting rod 19 penetrating through the connecting plate 18 is further arranged on the base 1 on one side of the screw rod 17, the screw rod is rotated, under the limitation of the limiting rod 19, the connecting plate 18 can only drive the traction ring 16 to move up and down along the height of the screw rod 17, so that the traction ring 16 is lifted and sleeved at the bottom of the test tube 8 during sorting, the traction ring 16 is lowered after sorting is finished so that the cup part 14 is detached from the bottom end of the tube part 13, and the convenient operation is realized while the structural function of the traction ring.

Preferably, be equipped with rubber circle 20 in the pull ring 16, the top of rubber circle 20 is for leaking hopper-shaped with test tube 8 bottom adaptation, and the setting of rubber circle 20 increases the frictional force of pull ring 16 and test tube 8 bottom on the one hand, prevents that test tube 8 bottom from breaking away from pull ring 16, and on the other hand provides a buffering for test tube 8 bottom, prevents that test tube 8 bottom and pull ring 16 inner wall from colliding with the damage.

Preferably, be equipped with bracing piece 21 on the base 1, bracing piece 21 is located between limiting plate 2 and the test tube 8, bracing piece 21 top is equipped with spacing ring 22, movable rod 3 run through spacing ring 22 mesopore and with spacing ring 22 sliding connection, play the effect of auxiliary stay to the mid portion of movable rod 3, prevent that movable rod 3 from being oppressed by tip test tube 8 and producing slight radian, guide movable rod 3 is normal to pass limiting plate 2 and is the horizontal motion.

Preferably, be equipped with a plurality of arcs 23 in the spacing ring 22, it is a plurality of become an annular after 23 splices of arc, it is a plurality of the annular internal diameter that 23 splices of arc is less than the diameter of movable rod 3, be connected with second spring 24 between 23 outsides of arc and the spacing ring 22 inboard, thereby 3 surface and 23 internal surface contact of arc of movable rod, because the annular internal diameter that 23 splices of arc is less than the diameter of movable rod 3, consequently after movable rod 3 penetrates a plurality of arcs 23 middle parts, thereby movable rod 3 outwards pushes away arc 23 extrusion second spring 24, makes 23 medial surfaces of arc and 3 surface in close contact with of movable rod under the effect of second spring 24, further guarantees the effect of spacing ring 22 to the auxiliary stay and the spacing guide of movable rod 3.

Preferably, one side that the arc 23 is close to the movable rod 3 is equipped with ball 25, through the contact of ball 25 with the surface of movable rod 3, makes movable rod 3 when horizontal motion, becomes movable rod 3 and the rolling friction of arc 23 with the sliding friction of movable rod 3 with ball 25, frictional force when reducing movable rod 3 and moving in spacing ring 22, reduces the friction loss when making the horizontal motion of movable rod 3 more smooth, the life of extension equipment.

Preferably, the base 1 is rotatably provided with a striking rod 26, the end of the striking rod 26 can contact with the support rod 21, the base 1 is provided with a second motor 27, the second motor 27 is in transmission connection with the striking rod 26 through a meshed bevel gear, the second motor 27 drives the striking rod 26 to slowly rotate, so that the end of the striking rod 26 regularly strikes the support rod 21, the support rod 21 vibrates after being struck and transmits the vibration to the movable rod 3 through the limit ring 22, and finally the vibration is transmitted to the test tube 8, so that the test tube 8 can also regularly vibrate in the shaking process to achieve the effect of vibrating screen, thereby further improving the efficiency of cell mass sorting and filtering, the rotation speed of the second motor 27 is slow, the striking rod 26 is made of an elastic material, the striking with large force cannot be caused to the support rod 21, the equipment is prevented from being damaged, and the support rod 21 cannot generate large vibration to affect the horizontal reciprocating motion of the movable rod 3, ensuring the normal use of the device.

Preferably, the cross-section of ejector pad 6 is the rhombus, the rhombus the contained angle of ejector pad 6 is the arc, the one end that connecting block 4 is close to ejector pad 6 also is the arc, makes ejector pad 6 length side, width side when promoting connecting block 4 and the conversion process of connecting block 4 contact softer to make the reciprocating motion of movable rod 3 more smooth and more steady.

By the device, the test tube 8 can be shaken and vibrated when the tumor cell mass is sorted and filtered, so that the sorting speed of the cell mass is increased, the sorting efficiency is improved, manual operation is not needed, the labor is saved, and the working efficiency is improved.

Claims

1. Tumour cell group's that oncology was used sorting filter equipment which characterized in that: comprises a base (1), a limiting plate (2) is arranged on the base (1), a movable rod (3) penetrating through the limiting plate (2) is arranged on the limiting plate (2), the movable rod (3) is connected with the limiting plate (2) in a sliding way,

one end of the movable rod (3) is provided with a connecting block (4), the movable rod (3) is sleeved with a first spring (5), the first spring (5) is positioned between the connecting block (4) and the limiting plate (2), the base (1) on one side of the limiting plate (2) is rotatably provided with a push block (6), the length and the width of the push block (6) are different, the outer side of the push block (6) is in contact with one end of the connecting block (4) far away from the movable rod (3), the base (1) is provided with a first motor (7), the first motor (7) is in transmission connection with the push block (6) through a meshed bevel gear,

one end of the movable rod (3) far away from the connecting block (4) is connected with a test tube (8) through a connecting piece (15), a filtering part is detachably arranged in the test tube (8), one side of the test tube (8) is communicated with an air outlet pipe (9),

the filter component comprises a first filter screen (10) and a second filter screen (11) which are bowl-shaped and arranged from top to bottom, the aperture of the first filter screen (10) is larger than that of the second filter screen (11), the outer sides of the first filter screen (10) and the second filter screen (11) are fixedly connected through a connecting rod (12), and the top end of the connecting rod (12) is detachably connected with the pipe orifice wall of the test tube (8).

2. The apparatus for sorting and filtering tumor cell mass for oncology department according to claim 1, wherein: the test tube (8) comprises a tube part (13) and a cup part (14), wherein the cup part (14) is in threaded connection with the bottom end of the tube part (13).

3. The apparatus for sorting and filtering tumor cell masses for oncology department according to claim 1 or 2, characterized in that: connecting piece (15) are joint bearing, test tube (8) bottom is equipped with traction ring (16), traction ring (16) are installed on base (1) through elevating gear, traction ring (16) are passed through elevating gear and can dismantle with test tube (8) bottom and be connected.

4. The apparatus for sorting and filtering tumor cell mass for use in oncology department according to claim 3, wherein: the lifting device comprises a screw rod (17) rotatably connected with the base (1), a connecting plate (18) is arranged on one side of the traction ring (16), the screw rod (17) penetrates through the connecting plate (18) and is in threaded connection with the connecting plate (18), and a limiting rod (19) penetrating through the connecting plate (18) is further arranged on the base (1) on one side of the screw rod (17).

5. The apparatus for sorting and filtering tumor cell mass for use in oncology department according to claim 4, wherein: be equipped with rubber circle (20) in pull ring (16), rubber circle (20) top is for leaking hopper-shaped that suits with test tube (8) bottom.

6. The apparatus for sorting and filtering tumor cell mass for oncology department according to claim 1, wherein: be equipped with bracing piece (21) on base (1), bracing piece (21) are located between limiting plate (2) and test tube (8), bracing piece (21) top is equipped with spacing ring (22), movable rod (3) run through spacing ring (22) mesopore and with spacing ring (22) sliding connection.

7. The apparatus for sorting and filtering tumor cell mass for use in oncology department according to claim 6, wherein: be equipped with a plurality of arcs (23) in spacing ring (22), it is a plurality of become an annular after arc (23) concatenation, it is a plurality of the annular internal diameter that arc (23) concatenation becomes is less than the diameter of movable rod (3), be connected with second spring (24) between arc (23) outside and spacing ring (22) inboard, movable rod (3) surface and arc (23) internal surface contact.

8. The apparatus for sorting and filtering tumor cell mass for use in oncology department according to claim 7, wherein: and a ball (25) is arranged on one side of the arc-shaped plate (23) close to the movable rod (3).

9. The device for sorting and filtering tumor cell masses for oncology department according to any one of claims 6 to 8, wherein: the base (1) is provided with a striking rod (26) in a rotating mode, the end portion of the striking rod (26) can be in contact with the supporting rod (21), the base (1) is provided with a second motor (27), and the second motor (27) is in transmission connection with the striking rod (26) through a bevel gear in meshing mode.

10. The apparatus for sorting and filtering tumor cell mass for oncology department according to claim 1, wherein: the cross section of the push block (6) is rhombic, the included angle of the push block (6) is arc-shaped, and one end, close to the push block (6), of the connecting block (4) is also arc-shaped.