CN114085759B - Tumor tissue separation and recovery device for organoid culture - Google Patents
Tumor tissue separation and recovery device for organoid culture Download PDFInfo
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- CN114085759B CN114085759B CN202111436268.6A CN202111436268A CN114085759B CN 114085759 B CN114085759 B CN 114085759B CN 202111436268 A CN202111436268 A CN 202111436268A CN 114085759 B CN114085759 B CN 114085759B
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- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 77
- 210000001519 tissue Anatomy 0.000 title claims abstract description 72
- 210000002220 organoid Anatomy 0.000 title claims abstract description 21
- 238000000926 separation method Methods 0.000 title description 9
- 238000011084 recovery Methods 0.000 title description 4
- 238000011010 flushing procedure Methods 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- 238000003756 stirring Methods 0.000 claims description 28
- 238000005192 partition Methods 0.000 claims description 23
- 239000000243 solution Substances 0.000 claims description 23
- 238000001914 filtration Methods 0.000 claims description 13
- 239000002002 slurry Substances 0.000 claims description 12
- 239000002504 physiological saline solution Substances 0.000 claims description 8
- 230000010355 oscillation Effects 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 7
- 238000004140 cleaning Methods 0.000 description 7
- 238000007667 floating Methods 0.000 description 4
- 239000012535 impurity Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
Abstract
The invention relates to the technical field of medical equipment, in particular to a device for separating and recovering tumor tissues for organoid culture, which comprises a negative pressure tractor, a separating tank, a collecting tank and a showering tank, wherein the collecting tank is connected with a separating tank pipeline; medical staff uses the negative pressure tractor to lead the tumor tissue to the inside of the separating tank, the flushing tank firstly uses normal saline to flush and filter the tumor tissue, and then the culture solution is led in, so that the tumor tissue floats in the culture solution, the collecting tank absorbs the culture solution containing the tumor tissue, the collection of the tumor tissue is completed, and the activity of the tumor tissue is ensured.
Description
Technical Field
The invention relates to the technical field of medical appliances, in particular to a device for separating and recovering tumor tissues for organoid culture.
Background
In recent years, the incidence of tumors is on the rise, and in statistics, 457 ten thousand new tumor patients in China in 2020 face a great challenge in tumor treatment. The organoid drug-sensitive technology is a new technology which is newly developed in recent years, separates and cultures tumor tissues of tumor patients, constructs organoids with genetic heterogeneity, and guides clinical treatment schemes by drug-sensitive analysis of the tumor organoids, so that a plurality of problems exist in how to separate and store tumor tissues with tissue activity, the recovery of the tumor tissues needs to be manually collected and cleaned by experimenters after the operation is finished, the burden of experimenters and the risk of bacteria infection are increased, and the tumor tissues lose activity after leaving the human body, so that the subsequent experimental results are affected.
Disclosure of Invention
The invention aims to provide a device for separating and recovering tumor tissues for organoid culture, and aims to solve the technical problems that in the prior art, after an operation is completed, medical staff is required to collect and clean the tumor tissues manually, the burden of the medical staff is increased, and the tumor is inactive after leaving a human body, so that the follow-up medical research is influenced.
In order to achieve the above object, the present invention provides a device for separating and recovering tumor tissue for organoid culture, the device capable of separating and recovering tumor tissue for organoid culture includes a negative pressure tractor, a separating tank, a collecting tank and a showering tank, the collecting tank is connected with the separating tank through a pipeline, the separating tank includes a first tank, a partition board, a rotary filter assembly, a vibrating assembly and a first motor, the partition board is disposed in the first tank, the first motor is disposed below the partition board, the rotary filter assembly is rotationally connected with an output end of the first motor and is disposed above the partition board, the vibrating assemblies are plural, each vibrating assembly is uniformly disposed between the partition board and the rotary filter assembly at intervals, a water outlet is disposed on an outer side wall of the first tank, the negative pressure tractor and the showering tank are both connected with a top end pipeline of the first tank, and the collecting tank is connected with a side wall of the first tank through a pipeline;
the rotary filtering assembly comprises a rotary rod, a filter plate, stirring blocks and stirring sheets, wherein the rotary rod is rotationally connected with an output shaft of the first motor and is positioned above the partition plate, the stirring blocks are two in number and are fixedly connected with the rotary rod and positioned on two sides of the rotary rod, the filter plate is fixedly connected with the rotary rod and positioned on the top end of the rotary rod, the stirring sheets are multiple in number, and each stirring sheet is fixedly connected with the filter plate and positioned above the filter plate;
every vibrate subassembly all includes spacing frame, roof, toper piece, movable rod, spring and push plate, spacing frame with baffle fixed connection, and be located the top of baffle, the roof with spacing frame fixed connection, and be located the top of spacing frame, toper piece with spacing frame sliding connection, and be located the inside of spacing frame, the spring set up in between toper piece with the roof, the movable rod with toper piece fixed connection, the movable rod passes the roof, and with roof sliding connection, push plate with movable rod fixed connection, and be located the top of movable rod.
Medical staff uses the negative pressure tractor to lead the tumor tissue to the inside of knockout drum, the flushing jar utilizes normal saline to drench and filter the tumor tissue earlier, and the rethread culture solution for tumor tissue floats in the culture solution, the collection jar absorbs the culture solution that contains tumor tissue, accomplishes the collection of tumor tissue, and guaranteed the activity of tumor tissue.
The negative pressure tractor comprises a pressure regulator, a suction pipe, a second tank body and a conical pipe, wherein the second tank body is arranged above the first tank body, the suction pipe is communicated with the second tank body, the pressure regulator is arranged above the second tank body, the conical pipe is fixedly connected with the second tank body and is positioned below the second tank body, the conical pipe is communicated with the first tank body, and an electromagnetic valve is arranged inside the conical pipe.
And adjusting suction force by using the pressure regulator, aligning the pipe orifice of the suction pipe with a part to be absorbed, absorbing tumor tissues, immersing the tumor tissues into the second tank body, opening an electromagnetic valve below after the absorption is completed, and dropping the tumor tissues into the first tank body along the conical pipe.
The novel flushing tank comprises a first tank body, a second tank body, a main pipeline, a water pump, a reversing valve, an auxiliary pipeline and an output pipe, wherein the first tank body is arranged on one side of the first tank body, a first cavity and a second cavity are respectively arranged in the first tank body, physiological saline is filled in the first cavity, culture solution is filled in the second cavity, two ends of the main pipeline are respectively communicated with the reversing valve and the first cavity, two ends of the auxiliary pipeline are respectively communicated with the second cavity and the reversing valve, two ends of the output pipe are respectively communicated with the water pump and the top end of the first tank body, and the water pump is connected with the reversing valve through the pipeline.
After the tumor tissue enters the first tank body, the water pump is started, physiological saline is pumped from the first cavity body and is sent to the first tank body, after the first tank body is stirred and filtered, the reversing valve switches the passage, and the water pump pumps culture solution from the second cavity body and sends the culture solution to the first tank body.
The shower tank further comprises a shower head, wherein the shower head is fixedly connected with the output pipe and is positioned in the first tank body.
When normal saline is sent to the first tank body, tumor tissues above the rotary filtering component are showered through the showering head.
The collection tank comprises a fourth tank body and a slurry pump, wherein two ends of the slurry pump are respectively connected with the first tank body and the fourth tank body through pipelines.
After the culture solution is sent into the first tank body, tumor tissues are dispersed and float in the culture solution, the slurry pump is used for pumping, and the culture solution containing the tumor tissues is sent into the fourth tank body.
The collecting tank further comprises a transfer cylinder, and the transfer cylinder is arranged in the fourth tank body.
Through setting up the transfer cylinder, the culture solution that contains tumor tissue is sent into the inside of transfer cylinder, conveniently carries out subsequent treatment.
According to the device for separating and recovering the tumor tissues for organoid culture, a medical staff pulls the tumor tissues into the separating tank by using the negative pressure tractor, the flushing tank firstly uses normal saline to flush the tumor tissues, in the flushing process, the first motor is started to drive the rotary filter assembly to rotate, the flushing area is enlarged, when the liquid level formed by the normal saline exceeds the tumor tissues on the rotary filter assembly, the water pump stops, the first motor continuously rotates, so that the stirring blocks on two sides of the rotating rod rotate, the stirring blocks sequentially push each conical block, the conical blocks push the pushing plates to move through the rotating rod, and due to the spring, the pushing plates can descend to form linear reciprocating motion, the pushing plates move, so that local oscillation is formed in the normal saline, the stirring plates stir the tumor tissues, the tumor tissues are reduced in volume and separated from impurities, and the filtering plates are not easy to block; after the filtration and separation are finished, the electromagnetic valve of the water outlet is opened, physiological saline and impurities are completely discharged and then are closed, and then the culture solution is led into the flushing tank through the flushing tank, so that the tumor tissue floats in the culture solution, the culture solution containing the tumor tissue is absorbed by the collecting tank, the collection of the tumor tissue is finished, and the activity of the tumor tissue is ensured.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a perspective view of an apparatus for the isolation and recovery of tumor tissue for organoid culture according to the present invention.
Fig. 2 is an enlarged view of a partial structure at a provided by the present invention.
Fig. 3 is a cross-sectional view of a separator tank provided by the present invention.
Fig. 4 is a cross-sectional view of an oscillating assembly provided by the present invention.
Fig. 5 is a cross-sectional view of a collection tank provided by the present invention.
1-negative pressure tractor, 2-knockout drum, 3-collecting drum, 4-drenching drum, 5-first drum, 6-baffle, 7-rotary filter assembly, 8-oscillating assembly, 9-first motor, 10-rotating rod, 11-filter plate, 12-toggle block, 13-stirring piece, 14-limit frame, 15-top plate, 16-conical block, 17-movable rod, 18-spring, 19-push plate, 20-water outlet, 21-pressure regulator, 22-suction tube, 23-second drum, 24-conical tube, 25-third drum, 26-main tube, 27-water pump, 28-reversing valve, 29-auxiliary tube, 30-output tube, 31-first cavity, 32-second cavity, 33-drenching head, 34-fourth drum, 35-slurry pump, 36-dump drum, 37-second motor, 38-blade, 39-drum, 40-cover plate, 41-guide frame, 42-floating plate, 43-water inlet, 44-cleaning port.
Detailed Description
Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative and intended to explain the present invention and should not be construed as limiting the invention.
In the description of the present invention, it should be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present invention and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention. Furthermore, in the description of the present invention, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
Referring to fig. 1 to 5, the present invention provides a device for separating and recovering tumor tissue for organoid culture, the device capable of separating and recovering tumor tissue for organoid culture includes a negative pressure tractor 1, a separating tank 2, a collecting tank 3 and a showering tank 4, the collecting tank 3 is connected with the separating tank 2 through a pipeline, the separating tank 2 includes a first tank body 5, a partition plate 6, a rotary filtering component 7, a vibrating component 8 and a first motor 9, the partition plate 6 is disposed in the first tank body 5, the first motor 9 is disposed below the partition plate 6, the rotary filtering component 7 is rotationally connected with an output end of the first motor 9 and is located above the partition plate 6, the vibrating components 8 are plural, each vibrating component 8 is uniformly disposed between the partition plate 6 and the rotary filtering component 7 at intervals, a water outlet 20 is disposed on an outer side wall of the first tank body 5, the negative pressure tractor 1 and the first tank 4 are disposed in the interior of the water outlet 20, and the electromagnetic valve are connected with a side wall of the first tank body 5 through a pipeline 3;
the rotary filter assembly 7 comprises a rotary rod 10, a filter plate 11, stirring blocks 12 and stirring sheets 13, wherein the rotary rod 10 is rotationally connected with an output shaft of the first motor 9 and is positioned above the partition plate 6, the stirring blocks 12 are two in number, the stirring blocks 12 are fixedly connected with the rotary rod 10 and are positioned on two sides of the rotary rod 10, the filter plate 11 is fixedly connected with the rotary rod 10 and is positioned at the top end of the rotary rod 10, the stirring sheets 13 are multiple in number, and each stirring sheet 13 is fixedly connected with the filter plate 11 and is positioned above the filter plate 11;
each oscillating assembly 8 comprises a limiting frame 14, a top plate 15, a conical block 16, a movable rod 17, a spring 18 and a pushing plate 19, wherein the limiting frame 14 is fixedly connected with the partition plate 6 and is located above the partition plate 6, the top plate 15 is fixedly connected with the limiting frame 14 and is located above the limiting frame 14, the conical block 16 is slidably connected with the limiting frame 14 and is located inside the limiting frame 14, the spring 18 is arranged between the conical block 16 and the top plate 15, the movable rod 17 is fixedly connected with the conical block 16, the movable rod 17 penetrates through the top plate 15 and is slidably connected with the top plate 15, and the pushing plate 19 is fixedly connected with the movable rod 17 and is located above the movable rod 17.
In this embodiment, a medical staff pulls tumor tissue to the inside of the separation tank 2 by using the negative pressure tractor 1, the flushing tank 4 firstly uses normal saline to flush the tumor tissue, in the flushing process, the first motor 9 is started to drive the rotary filter assembly 7 to rotate, the flushing area is enlarged, when the liquid level formed by the normal saline exceeds the tumor tissue on the rotary filter assembly 7, the water pump 27 stops, the first motor 9 continuously rotates, so that the stirring blocks 12 on two sides of the rotating rod 10 rotate, the stirring blocks 12 sequentially push each conical block 16, the conical blocks 16 push the pushing plates 19 to move through the rotating rod 10, the pushing plates 19 can descend to form linear reciprocating motion due to the springs 18, a plurality of pushing plates 19 move to form local oscillation in the normal saline, the stirring sheets 13 stir the tumor tissue, so that the tumor tissue is reduced in volume and separated from impurities, and the filter plate 11 is not easy to block; after the filtration and separation are completed, the electromagnetic valve of the water outlet 20 is opened, and the physiological saline and impurities are completely discharged and then closed; introducing the culture solution into the showering tank 4 to float the tumor tissues in the culture solution, and absorbing the culture solution containing the tumor tissues by the collecting tank 3 to collect the tumor tissues and ensure the activity of the tumor tissues; the whole PLC system control reduces the workload of medical staff.
Further, the negative pressure tractor 1 includes a pressure regulator 21, a suction tube 22, a second tank 23 and a conical tube 24, the second tank 23 is disposed above the first tank 5, the suction tube 22 is communicated with the second tank 23, the pressure regulator 21 is disposed above the second tank 23, the conical tube 24 is fixedly connected with the second tank 23 and is disposed below the second tank 23, the conical tube 24 is communicated with the first tank 5, and an electromagnetic valve is disposed inside the conical tube 24; the shower tank 4 comprises a third tank body 25, a main pipeline 26, a water pump 27, a reversing valve 28, an auxiliary pipeline 29 and an output pipe 30, wherein the third tank body 25 is arranged on one side of the first tank body 5, a first cavity 31 and a second cavity 32 are respectively arranged in the third tank body 25, physiological saline is filled in the first cavity 31, culture solution is filled in the second cavity 32, two ends of the main pipeline 26 are respectively communicated with the reversing valve 28 and the first cavity 31, two ends of the auxiliary pipeline 29 are respectively communicated with the second cavity 32 and the reversing valve 28, two ends of the output pipe 30 are respectively communicated with the water pump 27 and the top end of the first tank body 5, and the water pump 27 is in pipeline connection with the reversing valve 28; the shower tank 4 further comprises a shower head 33, and the shower head 33 is fixedly connected with the output pipe 30 and is located in the first tank body 5.
In this embodiment, the pressure regulator 21 is used to regulate the suction force, the orifice of the suction tube 22 is aligned to the part to be absorbed, the tumor tissue is immersed in the second tank 23, after the absorption is completed, the electromagnetic valve below is opened, the tumor tissue falls into the first tank 5 along the conical tube 24, after the tumor tissue enters the first tank 5, the water pump 27 is started, physiological saline is pumped from the first cavity 31 and sent into the first tank 5, after the first tank 5 completes stirring and filtering, the reversing valve 28 switches the passage, and the water pump 27 pumps culture fluid from the second cavity 32 and sends into the first tank 5; when the normal saline is sent to the first tank 5, the tumor tissue above the rotary filter assembly 7 is flushed through the flushing head 33.
Further, the collecting tank 3 comprises a fourth tank 34 and a slurry pump 35, and two ends of the slurry pump 35 are respectively connected with the first tank 5 and the fourth tank 34 in a pipeline manner; the collection tank 3 further includes a transfer cylinder 36, and the transfer cylinder 36 is disposed inside the fourth tank 34.
In this embodiment, after the culture solution is fed into the first tank 5, the tumor tissue is dispersed and floated in the culture solution, and the slurry is pumped by the slurry pump 35 to feed the culture solution containing the tumor tissue into the transfer cylinder 36, thereby facilitating the subsequent treatment.
Further, the separation tank 2 further includes a second motor 37 and a blade 38, the second motor 37 is disposed at the top end of the first tank 5 and located at one side of the negative pressure tractor 1, and the blade 38 is rotatably connected with the output end of the second motor 37 and located inside the first tank 5 and below the negative pressure tractor 1.
In this embodiment, when the tumor tissue enters the first tank 5 from the negative pressure tractor 1, the second motor 37 drives the blade 38 to rotate through the blade 38, so that the tumor tissue is scattered and falls above the rotating filter assembly 7, the primary separation is completed, and the subsequent separation effect is improved.
Further, the dump cylinder 36 includes a cylinder 39, a cover plate 40, a guide frame 41, and a floating plate 42, the cylinder 39 is disposed in the fourth tank 34, a water inlet 43 is disposed at a top end of the cover plate 40, the cover plate 40 is screwed to a top end of the cylinder 39, the guide frame 41 is fixedly connected to the cover plate 40 and is located below the water inlet 43, and the floating plate 42 is slidably connected to the guide frame 41 and is located in the guide frame 41.
In this embodiment, the culture solution containing tumor tissue is sent to the storage cylinder 36 by the slurry pump 35, enters the cylinder 39 through the water inlet hole 43 above the cover plate 40, and continuously rises along with the rise of the liquid level in the cylinder 39 until the water inlet hole 43 is blocked by the floating plate 42, and the sealing is completed, and the slurry pump 35 stops working.
Further, a cleaning opening 44 is formed in the side wall of the first tank body 5, the cleaning opening 44 is located above the partition plate 6, and an electromagnetic valve is arranged in the cleaning opening 44.
In this embodiment, after the separation is completed, the electromagnetic valve in the cleaning port 44 is opened, the water pump 27 continuously pumps normal saline, the first motor 9 is started, and the tumor tissue remained on the filter plate 11 is thrown out from the cleaning port 44, so as to complete the self-cleaning of the filter plate 11.
The above disclosure is only a preferred embodiment of the present invention, and it should be understood that the scope of the invention is not limited thereto, and those skilled in the art will appreciate that all or part of the procedures described above can be performed according to the equivalent changes of the claims, and still fall within the scope of the present invention.
Claims (6)
1. A device capable of separating and recovering tumor tissue for organoid culture, characterized in that,
the device capable of separating and recovering the tumor tissues for organoid culture comprises a negative pressure tractor, a separating tank, a collecting tank and a flushing tank, wherein the collecting tank is connected with the separating tank through a pipeline, the separating tank comprises a first tank body, a partition plate, a rotary filtering component, a vibration component and a first motor, the partition plate is arranged in the first tank body, the first motor is arranged below the partition plate, the rotary filtering component is rotationally connected with the output end of the first motor and is positioned above the partition plate, the vibration components are multiple, each vibration component is uniformly arranged between the partition plate and the rotary filtering component at intervals, a water outlet is formed in the outer side wall of the first tank body, an electromagnetic valve is arranged in the water outlet, the negative pressure tractor and the flushing tank are connected with the top end pipeline of the first tank body, and the collecting tank is connected with the side wall pipeline of the first tank body;
the rotary filtering assembly comprises a rotary rod, a filter plate, stirring blocks and stirring sheets, wherein the rotary rod is rotationally connected with an output shaft of the first motor and is positioned above the partition plate, the stirring blocks are two in number and are fixedly connected with the rotary rod and positioned on two sides of the rotary rod, the filter plate is fixedly connected with the rotary rod and positioned on the top end of the rotary rod, the stirring sheets are multiple in number, and each stirring sheet is fixedly connected with the filter plate and positioned above the filter plate;
each vibration assembly comprises a limiting frame, a top plate, a conical block, a movable rod, a spring and a pushing plate, wherein the limiting frame is fixedly connected with the partition plate and is positioned above the partition plate, the top plate is fixedly connected with the limiting frame and is positioned above the limiting frame, the conical block is slidably connected with the limiting frame and is positioned inside the limiting frame, the spring is arranged between the conical block and the top plate, the movable rod is fixedly connected with the conical block, the movable rod penetrates through the top plate and is slidably connected with the top plate, and the pushing plate is fixedly connected with the movable rod and is positioned above the movable rod; the first motor continuously rotates to enable the stirring blocks on two sides of the rotating rod to rotate, each conical block is sequentially pushed by the stirring blocks, the pushing plates are pushed to move by the conical blocks through the rotating rod, the pushing plates can descend to form linear reciprocating motion due to the springs, and a plurality of pushing plates move to enable local oscillation to be formed inside physiological saline.
2. A device capable of isolating and recovering tumor tissue for organoid culture according to claim 1,
the negative pressure tractor comprises a pressure regulator, a suction pipe, a second tank body and a conical pipe, wherein the second tank body is arranged above the first tank body, the suction pipe is communicated with the second tank body, the pressure regulator is arranged above the second tank body, the conical pipe is fixedly connected with the second tank body and is positioned below the second tank body, the conical pipe is communicated with the first tank body, and an electromagnetic valve is arranged inside the conical pipe.
3. A device capable of isolating and recovering tumor tissue for organoid culture according to claim 2,
the flushing tank comprises a third tank body, a main pipeline, a water pump, a reversing valve, an auxiliary pipeline and an output pipe, wherein the third tank body is arranged on one side of the first tank body, a first cavity and a second cavity are respectively arranged in the third tank body, physiological saline is filled in the first cavity, culture solution is filled in the second cavity, two ends of the main pipeline are respectively communicated with the reversing valve and the first cavity, two ends of the auxiliary pipeline are respectively communicated with the second cavity and the reversing valve, two ends of the output pipe are respectively communicated with the water pump and the top end of the first tank body, and the water pump is connected with the reversing valve pipeline.
4. A device capable of isolating and recovering tumor tissue for organoid culture according to claim 3,
the shower tank further comprises a shower head which is fixedly connected with the output pipe and is positioned in the first tank body.
5. A device capable of isolating and recovering tumor tissue for organoid culture according to claim 4,
the collecting tank comprises a fourth tank body and a slurry pump, wherein two ends of the slurry pump are respectively connected with the first tank body and the fourth tank body through pipelines.
6. A device capable of isolating and recovering tumor tissue for organoid culture according to claim 5,
the collecting tank further comprises a transfer cylinder, and the transfer cylinder is arranged in the fourth tank body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111436268.6A CN114085759B (en) | 2021-11-29 | 2021-11-29 | Tumor tissue separation and recovery device for organoid culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111436268.6A CN114085759B (en) | 2021-11-29 | 2021-11-29 | Tumor tissue separation and recovery device for organoid culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114085759A CN114085759A (en) | 2022-02-25 |
| CN114085759B true CN114085759B (en) | 2024-03-26 |
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| CN110917418A (en) * | 2019-12-26 | 2020-03-27 | 张洪亮 | Device capable of separating and recovering brain tumor tissues |
| CN211862865U (en) * | 2020-02-21 | 2020-11-06 | 东阳市人民医院 | Tumor pathology specimen collecting device |
| CN112358958A (en) * | 2020-11-23 | 2021-02-12 | 浙江省人民医院 | Sorting and filtering device for tumor cell mass for oncology department |
| CN212703443U (en) * | 2020-05-09 | 2021-03-16 | 贵州医科大学附属医院 | Device for separating and recovering brain tumor tissues |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110917418A (en) * | 2019-12-26 | 2020-03-27 | 张洪亮 | Device capable of separating and recovering brain tumor tissues |
| CN211862865U (en) * | 2020-02-21 | 2020-11-06 | 东阳市人民医院 | Tumor pathology specimen collecting device |
| CN212703443U (en) * | 2020-05-09 | 2021-03-16 | 贵州医科大学附属医院 | Device for separating and recovering brain tumor tissues |
| CN112358958A (en) * | 2020-11-23 | 2021-02-12 | 浙江省人民医院 | Sorting and filtering device for tumor cell mass for oncology department |
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