CN114053203A - Preparation method of exosome essence - Google Patents
Preparation method of exosome essence Download PDFInfo
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- CN114053203A CN114053203A CN202111216512.8A CN202111216512A CN114053203A CN 114053203 A CN114053203 A CN 114053203A CN 202111216512 A CN202111216512 A CN 202111216512A CN 114053203 A CN114053203 A CN 114053203A
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- water
- exosome
- phase pot
- stirring
- later use
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/606—Nucleosides; Nucleotides; Nucleic acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention discloses a preparation method of an exosome essence, which comprises the following steps of S1: mixing glutamic acid, arginine, threonine, alanine, lysine, valine and ribonucleic acid with water, and soaking uniformly for later use; s2: adding a small amount of sterilized water into hyaluronic acid for dilution for later use; s3: adding coagulated fat into the water phase pot, heating the water phase pot to 90-120 ℃, and keeping the temperature for more than 20 minutes; s4: cooling the mixed solution in the water phase pot to 20-25 ℃, and discharging part of water for later use; s5: adding glutamic acid, arginine, threonine, alanine, lysine, valine and ribonucleic acid into a water-phase pot, and stirring; wherein the stirring temperature is 20-40 ℃, and the stirring time is 50-80 minutes; s6: standing for 15-30 hours; s7: oligopeptide-3 and the water released in step S4 were added. The essence can prolong the attachment time of the exosome on the skin after being used, improve the efficiency of the exosome entering the body and better exert the function of the exosome.
Description
Technical Field
The invention relates to the technical field of production processes of essence, in particular to a preparation method of exosome essence.
Background
With the continuous improvement of living standard, people pay more and more attention to their own appearance and image after satisfying the problem of satiety, and the demand for skin care products is higher and higher, and besides the most basic effects of skin care and beautifying and concealing, people tend to pursue the change of their own skin by using skin care products, such as whitening, removing freckles, increasing the tension and elasticity of the skin, and the like. However, most of the existing cosmetic products contain many chemical components, which can cause certain stimulation to human skin, especially some chemical synthetic products also contain lead, alcohol, lemon oil, pigment, antiseptic, etc., which not only are very bad for human health, but also can cause skin relaxation, edema, thin horny layer, easy allergy, etc. after long-term use, especially the existing essence products are commonly added with active components to promote the absorption of skin nutrient components, which can temporarily relieve skin problems, but the skin becomes more fragile and easy to age after long-term use. Therefore, how to select green, healthy and safe cosmetics is of great significance.
Disclosure of Invention
The present invention is directed to solving, at least to some extent, one of the technical problems in the related art. To this end, an object of the present invention is to provide a method for preparing an exosome essence, comprising the following steps, S1: mixing glutamic acid, arginine, threonine, alanine, lysine, valine and ribonucleic acid with water, and soaking uniformly for later use; s2: adding a small amount of sterilized water into hyaluronic acid for dilution for later use; s3: adding coagulated fat into the water phase pot, heating the water phase pot to 90-120 deg.C, and maintaining the temperature for more than 20 min; s4: cooling the mixed solution in the water phase pot to 20-25 ℃, and discharging part of water for later use; s5: adding glutamic acid, arginine, threonine, alanine, lysine, valine and ribonucleic acid into a water-phase pot, and stirring; wherein the stirring temperature is 20-40 ℃, and the stirring time is 50-80 minutes; s6: standing for 15-30 hours; s7: adding oligopeptide-3 and the water discharged in the step S4, and stirring for 20-30 minutes.
As a preferred technical scheme of the invention: the proportion of the used raw materials per kilogram in the S1 is as follows: 98.7233% of water, 0.0416% of glutamic acid, 0.0123% of arginine, 0.0050% of threonine, 0.0044% of alanine, 0.0078% of lysine, 0.0032% of valine and 0.0024% of ribonucleic acid.
By adopting the technical scheme, the invention has the beneficial effects that: the sodium hyaluronate is added into the exosome, so that the exosome can be maintained to be stored at low temperature for a long time, the moisturizing capability of the skin can be improved after the exosome is used, the attachment time of the exosome on the skin is prolonged, the exosome is promoted to enter the skin, the efficiency of the exosome entering the body is improved, and the function of the exosome is better exerted.
Detailed Description
The technical solutions in the embodiments of the present invention will be described below clearly and completely in connection with the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a preparation method of an exosome essence, which comprises the following steps: s1: mixing glutamic acid, arginine, threonine, alanine, lysine, valine and ribonucleic acid with water, and soaking uniformly for later use;
s2: adding a small amount of sterilized water into hyaluronic acid for dilution for later use;
s3: adding coagulated fat into the water phase pot, heating the water phase pot to 90-120 ℃, and keeping the temperature for more than 20 minutes;
s4: cooling the mixed solution in the water phase pot to 20-25 ℃, and discharging part of water for later use;
s5: adding glutamic acid, arginine, threonine, alanine, lysine, valine and ribonucleic acid into a water-phase pot, and stirring; wherein the stirring temperature is 20-40 ℃, and the stirring time is 50-80 minutes;
s6: standing for 15-30 hours; s7: adding oligopeptide-3 and the water discharged in the step S4, and stirring for 20-30 minutes.
Further, the ratio of the used raw materials per kilogram in the S1 is as follows: 98.7233% of water, 0.0416% of glutamic acid, 0.0123% of arginine, 0.0050% of threonine, 0.0044% of alanine, 0.0078% of lysine, 0.0032% of valine and 0.0024% of ribonucleic acid.
The sodium hyaluronate is added into the exosome, so that the exosome can be maintained at low temperature for a long time, the moisturizing capability of skin can be improved after the exosome is used, the attachment time of the exosome on the skin is prolonged, the exosome is promoted to enter the skin, the efficiency of the exosome entering the body is improved, and the function of the exosome is better exerted.
Wherein, the exosome refers to: the cell secretion, namely the exosome secreted by the cells is added with the humectant, and is not chemically synthesized like a mask, so that the essence can also increase the moisturizing capability of the skin after being used, prolong the attachment time of the exosome on the skin and promote the exosome to enter the skin, thereby improving the efficiency of the exosome entering the body and better exerting the function of the exosome.
The embodiments of the present invention have been described in detail, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in the embodiments without departing from the principles and spirit of the invention, and still fall within the scope of the invention.
Claims (2)
1. A preparation method of an exosome essence is characterized by comprising the following steps: comprising the following steps, S1: mixing glutamic acid, arginine, threonine, alanine, lysine, valine and ribonucleic acid with water, and soaking uniformly for later use;
s2: adding a small amount of sterilized water into hyaluronic acid for dilution for later use;
s3: adding coagulated fat into the water phase pot, heating the water phase pot to 90-120 ℃, and keeping the temperature for more than 20 minutes;
s4: cooling the mixed solution in the water phase pot to 20-25 ℃, and discharging part of water for later use;
s5: adding glutamic acid, arginine, threonine, alanine, lysine, valine and ribonucleic acid into a water-phase pot, and stirring; wherein the stirring temperature is 20-40 ℃, and the stirring time is 50-80 minutes;
s6: standing for 15-30 hours; s7: adding oligopeptide-3 and the water discharged in the step S4, and stirring for 20-30 minutes.
2. The method for preparing an exosome essence according to claim 1, which is characterized in that: the proportion of the used raw materials per kilogram in the S1 is as follows: 98.7233% of water, 0.0416% of glutamic acid, 0.0123% of arginine, 0.0050% of threonine, 0.0044% of alanine, 0.0078% of lysine, 0.0032% of valine and 0.0024% of ribonucleic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111216512.8A CN114053203A (en) | 2021-10-19 | 2021-10-19 | Preparation method of exosome essence |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111216512.8A CN114053203A (en) | 2021-10-19 | 2021-10-19 | Preparation method of exosome essence |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114053203A true CN114053203A (en) | 2022-02-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111216512.8A Pending CN114053203A (en) | 2021-10-19 | 2021-10-19 | Preparation method of exosome essence |
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| Country | Link |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190005653A (en) * | 2017-07-07 | 2019-01-16 | 종근당건강 주식회사 | Cosmetic composition for skin regeneration |
| CN109431918A (en) * | 2018-12-30 | 2019-03-08 | 浙江因兆艾生物科技有限公司 | A kind of skin barrier is impaired to repair MASQUE APAISANT HYDRATANT and preparation method thereof |
| CN110123713A (en) * | 2019-05-28 | 2019-08-16 | 儒兰（福建）生物科技有限公司 | A kind of tax running water and its preparation process |
| CN111840124A (en) * | 2020-08-03 | 2020-10-30 | 广州市丽泽化妆品有限公司 | Oligopeptide water-sensitive skin beautifying mask, essence thereof and preparation method of essence |
| CN113274312A (en) * | 2021-06-02 | 2021-08-20 | 上海活彩生物科技有限公司 | Essence for improving skin aging and injury and preparation method thereof |
-
2021
- 2021-10-19 CN CN202111216512.8A patent/CN114053203A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20190005653A (en) * | 2017-07-07 | 2019-01-16 | 종근당건강 주식회사 | Cosmetic composition for skin regeneration |
| CN109431918A (en) * | 2018-12-30 | 2019-03-08 | 浙江因兆艾生物科技有限公司 | A kind of skin barrier is impaired to repair MASQUE APAISANT HYDRATANT and preparation method thereof |
| CN110123713A (en) * | 2019-05-28 | 2019-08-16 | 儒兰（福建）生物科技有限公司 | A kind of tax running water and its preparation process |
| CN111840124A (en) * | 2020-08-03 | 2020-10-30 | 广州市丽泽化妆品有限公司 | Oligopeptide water-sensitive skin beautifying mask, essence thereof and preparation method of essence |
| CN113274312A (en) * | 2021-06-02 | 2021-08-20 | 上海活彩生物科技有限公司 | Essence for improving skin aging and injury and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 国家药品监督管理局: "极谷护肤青春精华液" * |
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