CN1140471C - Process for decolouring by co-metabolism of immobilized bionts - Google Patents
Process for decolouring by co-metabolism of immobilized bionts Download PDFInfo
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- CN1140471C CN1140471C CNB011294965A CN01129496A CN1140471C CN 1140471 C CN1140471 C CN 1140471C CN B011294965 A CNB011294965 A CN B011294965A CN 01129496 A CN01129496 A CN 01129496A CN 1140471 C CN1140471 C CN 1140471C
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Abstract
The present invention provides a decolourization method for co-metabolism of immobilized bionts, which comprises the following steps: a nutrient solution is added in non-sterilized water to generate a bacterial suspension; the bacterial suspension is supplied to an upstream type fixing bed reactor whose filling material is a concave-convex rod stone; carbon source and nitrogen source substances can be added in water to be treated. The present invention can make waste water which comprises azo compounds generate biodegradation decolourization. Decolorization superiority fungus in the present invention has short acclimation time, high immobilized decolorization fungus efficiency on the surface of the concave-convex rod stone and simple and reliable immobilized method. The present invention can be used for treating printing and dyeing waste water which comprises 50 mg/L of dye, and the average chroma removing rate can reach about 80%.
Description
Technical field
The invention belongs to a kind of method, relate in particular to a kind of method that azoic dyestuff is adopted decolouring by co-metabolism of immobilized bionts the azoic dyestuff biological decolouring.
Background technology
Treatment of dyeing and printing method commonly used has physical chemistry method and biological process.Materialization technology commonly used at present has adsorption bleaching technology, coagulating sedimentation technology, chemical oxidation technology, ion exchange technique, ultra-filtration membrane decolouring technology etc.Biochemical process mainly contains aerobic treatment, anaerobic treatment and aerobic anaerobic process etc.The investigator generally handles the bacterial strain of isolating the energy degradation of dye the structures from dying industrial wastewater, through adding active sludge after the enlarged culturing or adopting the immobilized cell method bacterial strain to be fixed on the carrier to improve treatment effect (application--the Xu Wendong etc. of microorganism in waste water containing dye is handled, Techniques and Equipment for Environmental Pollution Control, 2000, Vol.1, No.2); 1996, P.K.wong and P.Y.Yuen are at " WaterResearch " Vol.30, No.7 delivers degraded and the decolouring that is entitled as Klebsiella Pneumoniae RS-13 paramethyl red, reported the bacterial strain of from contain dyestuff mud, isolating a kind of being named as " KlebsiellaPneumoniae RS-13 ", under aerobic condition, can produce complete degradation and decolorization 100ppm Acid Red B dyestuff; (microorganism is to the decolouring research-Biotechnology Letters of printing and dyeing industrial waste water for people's such as Poonam Nigam result of study, Vol.18, No1,1996) show, the bacterial strain Ensure Liquid thing (lactose outside of isolated PDW by name, starch) under the condition, printing and dyeing mill's effluent color dilution is removed reached 76%; People such as Niamh Kirby are at Biotechnology Letters, Vol.17, and No7,1995 deliver and are entitled as the decolouring research of Phanerochaete Chrysosporium to the simulation dyestuff, and the result shows that this bacterial classification can make dyestuff decolour fully in 7 days; 1996, Darren.A etc. (Biotechnology letters 1996 Vol.18 No.5:527-530) adopt upflow type anaerobic bed (UAF), adopt rubble be carrier fixedly the PDW bacterium of decolouring also obtained result preferably.The fixed growth diagram of system reveals the pollutant removal stronger than suspension growth system.
The common ground of above research is, the investigator separates bacterial classification from the waste water that contains dyestuff, mud, and adopting the target dyestuff is that sole carbon source carries out orientation domestication, induces to produce corresponding degradation enzyme system, screens and obtains high-efficiency strain.The time of general needs of this method 1 month even some months.Obtain the single bacterial strain of efficient decolorizing, after enrichment culture, be used for the decolouring of dyestuff.Cultivation, domestication time that this process need is long.
Summary of the invention
The objective of the invention is to overcome the shortcoming of prior art, the method for the decolouring by co-metabolism of immobilized bionts that a kind of dominant bacteria domestication time of decolouring is short, steady time is long, process for fixation is simple effectively, expense is cheap is provided.
For achieving the above object, the method for a kind of decolouring by co-metabolism of immobilized bionts provided by the invention, its step comprises:
Do not add nutritive medium in the aqua sterilisa, producing bacteria suspension;
Bacteria suspension is fed in the upflow fixed bed reactor that weighting material is an attapulgite;
Handle adding carbon source and nitrogen source in the former water in desire.
Not aqua sterilisa of the present invention is tap water, sanitary sewage, dyeing waste water.
Nutritive medium of the present invention is peptone or glucose.
Attapulgite of the present invention is the particle of diameter 2-5 millimeter, makes in 3 hours in 750 ℃ of calcinations.
Upflow fixed bed reactor water inlet chemical oxygen demand (COD) of the present invention is at 200 grams per liters, and hydraulic detention time is 30-120 minute.
Carbon source of the present invention and nitrogen source are peptone, glucose, sanitary sewage and/or dyeing and printing sewage.
Adding peptone of the present invention is 2 with the ratio of desiring treating water: 5-3: 5 (weight ratios).
Adding glucose of the present invention is 10 with the ratio of desiring treating water: 5-50: 5 (weight ratios).
Description of drawings
By following embodiment and the detailed description with the accompanying drawing of providing, will have further the present invention and to understand, embodiment given below only is used for illustrating the present invention, rather than is used for limiting the present invention.
Accompanying drawing 1 is the stereoscan photograph on concave-convex rod particle surface;
Accompanying drawing 2 is the stereoscan photograph of bacillus cereus at the adsorption of immobilization on Attapulgite surface;
Accompanying drawing 3 is the saturated extent of adsorption of blank concave-convex rod particle to Acid Red B;
Accompanying drawing 4 is the variation of water outlet biomass behind the fixed-bed reactor immobilization bacterium;
Accompanying drawing 5 moves near 1384 hours Attapulgite surface scan electromicroscopic photographs the water outlet for fixed-bed reactor.
Embodiment
By operation steps, peptone or glucose are added not in the aqua sterilisa, nutrient concentrations is more than 400mg/L, produce bacteria suspension, feeding is in the upflow fixed bed reactor of filler with the Attapulgite ore, leaves standstill the immobilization of finishing the decolouring bacterium behind the 48h, and this immobilization post can make and contain azo dyes waste water and produce metabolic degradation decolorization altogether, the electromicroscopic photograph of immobilization filler and immobilized thallus such as Fig. 1, shown in Figure 2.As seen from Figure 2, metabolism decolouring dominant bacteria-bacillus cereus has the good adsorption immobilization characteristic on the surface of concave-convex rod particle altogether.Add 20g Φ 2-5mm concave-convex rod particle in culture dish, add the 30ml phosphate buffered saline buffer, the 10ml bacteria suspension is inserted in the sterilization back, behind 20 ℃ of Static Adsorption 48h, adopts the plate dilution method of counting, bacterial number in the bacteria suspension before and after the counting absorption.In adsorption of immobilization bacterial classification process, may there be the process of growth, breeding in the bacterium in the suspension, but under the condition of no exogenous nutrition, the bacillus cereus poor growth, adsorption rate is far longer than increment.Therefore, the adsorption rate in the absorption of the Attapulgite shown in the table 1 bacillus cereus amount is minimum adsorption rate.
Table 1
| Bacterial number absorption bacterium amount adsorption rate or immobilization in the bacteria suspension (individual/ml) before the absorption of (individual/the g Attapulgite) rate (%) after the absorption | |
| Parallel 1 parallel 2 | 3.9×10 7 5.1×10 6 6.8×10 7 86.9 3.9×10 7 4.7×10 6 6.9×10 7 87.9 |
Because efficient decolorizing bacteria is removed to being total to metabolic way the degraded of dye composition, thereby in treating processes, needing in former water, to add the carbon source and the nitrogen source of a definite form, carbon source described here and nitrogen source are peptone, glucose, sanitary sewage and/or dyeing and printing sewage.Processing contains the 50mg/L dye printing and dyeing wastewater, and residence time 120min is a peptone when adding nutrition, and the ratio of concentration and dyestuff is 2: 5 to 4: 5 o'clock, and the average chrominance clearance can reach 76.43%.Improve nutraceutical ratio in the water inlet, the decolouring treatment effect improves.Consider to improve and nutraceutically raise the cost than regular meeting, requirement according to the decolouring processing, the ratio that can make the concentration of adding the nutrition peptone and dyestuff is between 2: 5 to 3: 5, and the concentration of interpolation nutrition glucose and the ratio of dyestuff are between 10: 5 to 50: 5.
The method of decolouring by co-metabolism of immobilized bionts of the present invention has good common metabolism biological decoloration performance under the condition of preferred version of the present invention.
For the target dye composition has carried out the adsorption test of blank concave-convex rod particle to dyestuff, the results are shown in Figure 3 with the Acid Red B, this figure shows that Attapulgite is 0.049mg/g to the static saturated extent of adsorption of Acid Red B.This shows that concave-convex rod particle is very low to the eliminating rate of absorption of Acid Red B.
Find out that by the variation tendency of water outlet biomass under Fig. 4 different in flow rate the Attapulgite filler is higher to the immobilization efficiency of decolouring bacterium, being in operation the thalline leakage do not occur.Hydraulic detention time is that 30min, 60min and three common metabolism decolorizing column water outlet suspended solidss of 120min reach the highest at 12h, goes to zero behind the 48h.
Embodiment 1:
The 800mg/L peptone is allocated in the tap water, fed upflow fixed bed reactor, produce bacteria suspension, feeding weighting material is in the upflow fixed bed reactor of attapulgite, leave standstill 48h, this attapulgite is the particle of diameter 2-5 millimeter, and makes in 3 hours in 750 ℃ of calcinations.After finishing the bacterium immobilization, will contain Acid Red B 50mg/L and the different concns peptone is allocated in the tap water, and handle former water as desire and decolour.The reactor cylinder is cylindric synthetic glass post, high 100cm, internal diameter 7.2cm, the position, bottom is hemispherical, is provided with water inlet pipe, and bottom and main body junction are provided with multihole distributor, rise and support filler effect and moisturizing effects, water vent is apart from capital 20cm, and the water inlet oxygen requirement is 200mg/L.Water distribution concentration and corresponding color removal the results are shown in Table 2.
Table 2 (unit: %)
| Water distribution peptone: Acid Red B | Hydraulic detention time 30min | Hydraulic detention time 60min | Hydraulic detention time 120min | ||||||
| Minimum value | Maximum value | Mean value | Minimum value | Maximum value | Mean value | Minimum value | Mean value | | |
| 2∶5 | 46.20 | 46.20 | 46.20 | 62.15 | 62.15 | 62.15 | 74.40 | 74.40 | 74.40 |
| 3∶5 | 32.01 | 83.20 | 63.17 | 51.77 | 85.24 | 68.13 | 67.33 | 87.30 | 79.61 |
It is dyeing waste water that desire is handled former water, and all the other operational conditions the results are shown in Table 3 with among the embodiment 1.
Table 3 (unit: %)
| Water distribution peptone: Acid Red B | Hydraulic detention time 30min | Hydraulic detention time 60min | Hydraulic detention time 120min | ||||||
| Minimum value | Maximum value | Mean value | Minimum value | Maximum value | Mean value | Minimum value | Maximum value | Mean value | |
| 3∶5 | 53.33 | 79.92 | 74.67 | 54.36 | 81.55 | 73.01 | 68.90 | 82.44 | 78.55 |
| 2∶5 | 66.77 | 83.06 | 73.73 | 69.51 | 83.25 | 75.18 | 76.97 | 83.79 | 79.14 |
Embodiment 3
It is dirty for life that desire is handled former water, and all the other operational conditions the results are shown in Table 4 with water embodiment 1.
Table 4 (unit: %)
| Water distribution peptone: Acid Red B | Hydraulic detention time 30min | Hydraulic detention time 60rmin | Hydraulic detention time 120min | ||||||
| Minimum value | Maximum value | Mean value | Minimum value | Maximum value | Mean value | Minimum value | Maximum value | Mean value | |
| 3∶5 | 54.41 | 79.51 | 73.82 | 56.71 | 82.56 | 75.89 | 63.86 | 86.67 | 79.92 |
| 2∶5 | 67.72 | 81.45 | 72.14 | 67.43 | 85.78 | 74.23 | 68.91 | 83.62 | 76.45 |
Embodiment 4
Nutrition and carbon source are glucose, and all the other operational conditions the results are shown in Table 5 with embodiment 1.
Table 5 (unit: %)
| Water distribution glucose: Acid Red B | Hydraulic detention time 30min | Hydraulic detention time 60min | Hydraulic detention time 120min | ||||||
| Minimum value | Maximum value | Mean value | Minimum value | Maximum value | Mean value | Minimum value | Maximum value | | |
| 10∶5 | 47.45 | 47.45 | 47.45 | 61.45 | 61.45 | 61.45 | 76.35 | 76.35 | 76.46 |
| 20∶5 | 31.89 | 82.15 | 64.56 | 49.87 | 84.36 | 70.16 | 68.99 | 88.45 | 81.45 |
| 50∶5 | 68.79 | 87.56 | 76.56 | 78.74 | 85.14 | 80.14 | 85.14 | 88.31 | 85 54 |
It is the upflow fixed bed reactor of attapulgite that the present invention adopts weighting material, very abundant mutually through the long-time running artifact, accompanying drawing 5 moves near 1384 hours Attapulgite surface scan electromicroscopic photographs the water outlet for fixed-bed reactor, as can be seen, through long-time running, decolouring bacterium-bacillus cereus still keeps predominance in the reactor.
Claims (3)
1, a kind of method of decolouring by co-metabolism of immobilized bionts is characterized in that, comprises the following steps:
Do not add nutritive medium in the aqua sterilisa, producing bacteria suspension;
Bacteria suspension is fed in the upflow fixed bed reactor that weighting material is an attapulgite;
In handling former water, add carbon source and nitrogen source;
Described not aqua sterilisa is tap water, sanitary sewage, dyeing waste water;
Described nutritive medium is peptone or glucose;
Described carbon source and nitrogen source are protein, glucose, sanitary sewage and/or dyeing and printing sewage;
Described upflow fixed bed reactor water inlet chemical oxygen demand (COD) is at 200 grams per liters, and hydraulic detention time is 30-120 minute;
Described attapulgite is the particle of diameter 2-5 millimeter, makes in 3 hours in 750 ℃ of calcinations.
2, the method for decolouring by co-metabolism of immobilized bionts according to claim 1 is characterized in that, described adding peptone and desire are handled the ratio of former water and counted 2 by weight: 5-3: 5.
3, the method for decolouring by co-metabolism of immobilized bionts according to claim 1 is characterized in that, described adding glucose counts 10 by weight with the ratio of desiring treating water: 5-50: 5.
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| CNB011294965A CN1140471C (en) | 2001-06-21 | 2001-06-21 | Process for decolouring by co-metabolism of immobilized bionts |
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| CNB011294965A CN1140471C (en) | 2001-06-21 | 2001-06-21 | Process for decolouring by co-metabolism of immobilized bionts |
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| CN1140471C true CN1140471C (en) | 2004-03-03 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP4536158B1 (en) * | 2010-04-15 | 2010-09-01 | 三木理研工業株式会社 | Colored wastewater treatment method and colored wastewater treatment apparatus used in the method |
| CN105800775B (en) * | 2016-05-20 | 2019-02-15 | 浙江新三印印染有限公司 | A kind of discoloration method of wastewater in textile printing and dyeing industry |
| CN107674869A (en) * | 2017-11-06 | 2018-02-09 | 中国科学院寒区旱区环境与工程研究所 | A kind of preparation, measure and the experimental method of attapulgite immobilization oil degradation microbial inoculum |
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