CN114045695B - Compound biological enzyme preparation and preparation method and application thereof - Google Patents
Compound biological enzyme preparation and preparation method and application thereof Download PDFInfo
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- CN114045695B CN114045695B CN202111304371.5A CN202111304371A CN114045695B CN 114045695 B CN114045695 B CN 114045695B CN 202111304371 A CN202111304371 A CN 202111304371A CN 114045695 B CN114045695 B CN 114045695B
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
- D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21B—FIBROUS RAW MATERIALS OR THEIR MECHANICAL TREATMENT
- D21B1/00—Fibrous raw materials or their mechanical treatment
- D21B1/04—Fibrous raw materials or their mechanical treatment by dividing raw materials into small particles, e.g. fibres
- D21B1/12—Fibrous raw materials or their mechanical treatment by dividing raw materials into small particles, e.g. fibres by wet methods, by the use of steam
- D21B1/14—Disintegrating in mills
- D21B1/16—Disintegrating in mills in the presence of chemical agents
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C9/00—After-treatment of cellulose pulp, e.g. of wood pulp, or cotton linters ; Treatment of dilute or dewatered pulp or process improvement taking place after obtaining the raw cellulosic material and not provided for elsewhere
- D21C9/10—Bleaching ; Apparatus therefor
- D21C9/1026—Other features in bleaching processes
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C9/00—After-treatment of cellulose pulp, e.g. of wood pulp, or cotton linters ; Treatment of dilute or dewatered pulp or process improvement taking place after obtaining the raw cellulosic material and not provided for elsewhere
- D21C9/10—Bleaching ; Apparatus therefor
- D21C9/1026—Other features in bleaching processes
- D21C9/1042—Use of chelating agents
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
- D21C9/00—After-treatment of cellulose pulp, e.g. of wood pulp, or cotton linters ; Treatment of dilute or dewatered pulp or process improvement taking place after obtaining the raw cellulosic material and not provided for elsewhere
- D21C9/10—Bleaching ; Apparatus therefor
- D21C9/16—Bleaching ; Apparatus therefor with per compounds
- D21C9/163—Bleaching ; Apparatus therefor with per compounds with peroxides
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- D—TEXTILES; PAPER
- D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
- D21D—TREATMENT OF THE MATERIALS BEFORE PASSING TO THE PAPER-MAKING MACHINE
- D21D1/00—Methods of beating or refining; Beaters of the Hollander type
- D21D1/20—Methods of refining
Abstract
The invention discloses a compound biological enzyme preparation and a preparation method and application thereof, wherein the biological enzyme preparation comprises 30-35% of endo-cellulase (by mass percent); 20-25% of exocellulase; 2-5% of sodium chloride; 1-3% of potassium sorbate; 0-12% of deionized water. During preparation, deionized water is added into a stirring tank, sodium chloride, pectinase and laccase are added into the stirring tank according to a certain proportion and stirred, then endo-cellulase, exo-cellulase and xylanase are continuously stirred, and potassium sorbate is added according to a certain proportion and stirred to obtain the compound bio-enzyme preparation. The bio-enzyme preparation is used for preparing chemi-mechanical pulp, and when the compound bio-enzyme preparation is used for treating wood chips, fibers can be activated to loosen fiber structures, and the internal decomposition of the fibers promotes the water absorption swelling and the fibrosis degree of the fibers in the pulp grinding process, so that the pulp grinding is easier, and the energy consumption of the pulp grinding is saved. Meanwhile, the compound biological enzyme preparation can remove part of lignin in the wood chips, improve the whiteness of the paper pulp and reduce the dosage of chemicals.
Description
Technical Field
The invention relates to the technical field of papermaking, in particular to a compound biological enzyme preparation and a preparation method and application thereof.
Background
With the further increase of economy in China, the living standard of people is continuously improved, the demand of people on paper products is also continuously increased, and the demand of the market can not be met by high-quality wood raw materials. Meanwhile, the development of the paper industry in China is rapid, the total paper yield is also rapidly increased, and the supply shortage of paper making raw materials becomes an important factor restricting the development of the paper industry in China. Along with the rising of fossil energy in the global scope, the price is continuously increased, so for high-yield pulp production enterprises, the increasing importance is brought to the reduction of electricity consumption of pulping, the improvement of high-yield pulp performance while saving production cost, and the expansion of the application range of paper pulp.
The chemi-mechanical pulp has the advantages of high yield, high bulk, good stiffness and the like, so that the chemi-mechanical pulp is more and more widely applied to the field of papermaking. In recent years, the biological enzyme technology is widely applied in the paper-making industry, the pollution of pulping and paper-making processes to the environment can be reduced by using the biological enzyme treatment, the product performance is improved, and the biological enzyme treatment method has double values of environmental protection and reality.
Chinese patent application CN108374292A provides a production process for preparing chemi-mechanical pulp by using a specific composite biological enzyme preparation, wherein the specific biological enzyme preparation is composed of glutamate dehydrogenase, citrate synthase, bacillus subtilis freeze-dried powder, bacillus aceticus freeze-dried powder, organic acid nutrient solution and water, the biological enzyme is added twice during wood chip thread rolling, but the enzymolysis reaction temperature reaches about 80 ℃, the temperature of a chemi-mechanical pulp pulping system is high, the utilization efficiency of the biological enzyme is low along with the stirring and shearing processes, and the pulping energy consumption is still high.
Disclosure of Invention
In view of the above defects in the prior art, the present invention aims to provide a compound bio-enzyme preparation, and a preparation method and applications thereof.
The purpose of the invention is realized by the following technical scheme:
a compound biological enzyme preparation: the biological enzyme preparation (mass percentage) comprises,
30-35% of endo-cellulase;
20-25% of exo-cellulase;
2-5% of sodium chloride;
1-3% of potassium sorbate;
0 to 12 percent of deionized water.
Preferably, the preparation further comprises 15-20% of xylanase; 10-15% of pectinase. The xylanase and the pectinase can well remove pectin in wood chips, degrade xylan of hemicellulose in the wood chips and enable the wood chip structure to be loose.
Preferably, the formulation further comprises laccase in an amount of 10-15%. The laccase can degrade lignin in wood chips.
Preferably, the enzyme activity of the endo-cellulase is 15000-20000u/ml; the enzyme activity of the exo-cellulase is 15000 to 20000u/ml.
Preferably, the enzyme activity of the xylanase is 20000-25000u/ml, and the enzyme activity of the pectinase is 20000-25000u/ml.
Preferably, the laccase has enzyme activity of 30000-35000u/ml.
Preferably, the preparation method of the compound bio-enzyme preparation comprises the following steps of firstly adding deionized water into a stirring tank, proportionally adding sodium chloride, pectinase and laccase into the stirring tank for stirring, then continuously stirring the endo-cellulase, the exo-cellulase and the xylanase, and finally proportionally adding potassium sorbate for stirring to obtain the compound bio-enzyme preparation.
Preferably, the application of the compound biological enzyme preparation is used for preparing chemi-mechanical pulp.
Preferably, the preparation of the bio-enzyme preparation into chemi-mechanical pulp comprises the following steps:
s1, preparing materials: screening the wood chips to a wood chip bin;
s2, biological enzyme pretreatment: pretreating wood chips entering a wood chip bin, wherein the condition of biological enzyme pretreatment is that the pH is 6-8, the temperature is 30-70 ℃, the dosage of a biological enzyme preparation is 200 g/absolute dry pulp, and the reaction time is more than 48 hours; diluting the compound biological enzyme preparation by 100 times with clear water, uniformly spraying the diluted biological enzyme preparation on wood chips entering a wood chip bin, and storing the wood chips sprayed with the biological enzyme in the wood chip bin for more than 48 hours;
s3, washing: washing the wood chips treated in the S2 and presteaming under normal pressure;
s4, screw extrusion: carrying out screw extrusion on the wood chips treated in the step S3 to obtain extruded pulp;
s5, chemical impregnation: taking alkaline hydrogen peroxide solution as impregnation liquid medicine, and chemically impregnating the extruded slurry;
s6, first-stage coarse grinding: carrying out normal-pressure grinding on the slurry of the S5, wherein the mass concentration of the slurry is 20-25%;
s7, bleaching: performing supplementary bleaching on the slurry of the S6; the solvent of the bleaching type liquid medicine is water, the solutes comprise hydrogen peroxide, sodium hydroxide, sodium silicate and DTPA, and the ratio of the using amount of each solute to the oven-dried mass of the slurry is as follows: 3 to 4 percent of hydrogen peroxide, 2 to 4 percent of sodium hydroxide, 3.0 percent of sodium silicate and 0.5 percent of DTPA;
s8, secondary fine grinding: and (4) carrying out normal-pressure pulping on the pulp obtained in the step (S7) to prepare chemi-mechanical pulp, and respectively carrying out latency elimination, washing, screening and purification on the chemi-mechanical pulp to obtain paper pulp for testing the performance of the paper pulp, wherein the mass concentration of the pulp is 20-25%. The latency elimination conditions are as follows: the latency-eliminating temperature is above 80 ℃, and the latency-eliminating time is 10-30min.
Preferably, the temperature of the normal-pressure presteaming in the S3 is 90-105 ℃, and the time is 10-20min; and in the S4, double-helix extrusion is adopted for the screw extrusion, wherein the opening degree of an inlet is 20mm, and the opening degree of an outlet is 17mm. The extrusion temperature is 45-60 ℃; the solvent for soaking the liquid medicine in the S5 is water, the solutes comprise hydrogen peroxide, sodium hydroxide, sodium silicate and DTPA, and the ratio of the dosage of each solute to the oven-dried mass of the slurry is as follows: the content of hydrogen peroxide is 2-3%, the content of sodium hydroxide is 2-3%, the content of sodium silicate is 1.0%, the content of DTPA is 0.5%, the solute also comprises magnesium sulfate, and the content of magnesium sulfate is 0.1-0.2% of the oven-dried mass of the slurry; in S5, the mass concentration of the slurry in the dipping liquid medicine is 20-25%, the dipping time is 40-60min, and the dipping temperature is 70-90 ℃; the solute in the S7 also comprises magnesium sulfate, and the dosage of the magnesium sulfate is 0.1% -0.2% of the oven-dried mass of the slurry.
The invention has the following outstanding effects: the wood chips treated by the compound biological enzyme preparation can activate fibers to loosen fiber structures, promote the water absorption swelling and the fine fiber degree of the fibers in the pulping process by decomposing the inside of the fibers, make the pulping easier and save the pulping energy consumption. Meanwhile, the compound biological enzyme preparation can remove part of lignin in the wood chips, improve the whiteness of the paper pulp and reduce the dosage of chemicals.
The following detailed description of the embodiments of the present invention is provided in connection with the examples to facilitate understanding and understanding of the technical solutions of the present invention.
Detailed Description
The invention discloses a compound biological enzyme preparation, a preparation method and application thereof, wherein the pulping enzyme preparation is as follows:
the formula A of the compound biological enzyme preparation is as follows: 30% of endo-cellulase (the enzyme activity is 15000-20000 u/ml); 20% of exo-cellulase (the enzyme activity is 15000-20000 u/ml); 15% of xylanase (the enzyme activity is 20000-25000 u/ml); 12% of laccase (the enzyme activity is 30000-35000 u/ml); 13% of pectinase (the enzyme activity is 20000-25000 u/ml); 1% of potassium sorbate and 4% of sodium chloride; 5% of deionized water.
The formula B of the compound biological enzyme preparation is as follows: 32% of endo-cellulase (the enzyme activity is 15000-20000 u/ml); 22% of exo-cellulase (the enzyme activity is 15000-20000 u/ml); 15% of xylanase (the enzyme activity is 20000-25000 u/ml); 12% of laccase (the enzyme activity is 30000-35000 u/ml); 13% of pectinase (the enzyme activity is 20000-25000 u/ml); 1% of potassium sorbate and 3% of sodium chloride; and 2% of deionized water.
The formula C of the compound biological enzyme preparation is as follows: 35% of endo-cellulase (the enzyme activity is 15000-20000 u/ml); 25% of exo-cellulase (the enzyme activity is 15000-20000 u/ml); 12% of xylanase (the enzyme activity is 20000-25000 u/ml); 10% of laccase (the enzyme activity is 30000-35000 u/ml); 10% of pectinase (the enzyme activity is 20000-25000 u/ml); 1% of potassium sorbate and 4% of sodium chloride; 3% of deionized water.
The production process comprises the following steps:
example 1: poplar APMP
Storing poplar chips cut according to standard specifications, and respectively using clear water to mix the biological enzyme preparations of the formulas A, B and C according to the ratio of 1: diluting at 100 proportion. The diluted enzyme preparation solution is uniformly sprayed on wood chips of a feeding bin, 0.2 kg of compound biological enzyme preparation is used for pulp per ton, the wood chips sprayed with the enzyme preparation solution react in the bin for 48 hours for washing, steaming and screw extrusion, the pulp after screw extrusion is chemically impregnated, the dosage of hydrogen peroxide in the impregnating process is 20 kg/ton pulp, the dosage of caustic soda is 35 kg/ton pulp, the dosage of sodium silicate is 30 kg/ton pulp, the dosage of DTPA is 5 kg/ton pulp, and the dosage of magnesium sulfate is 2 kg/ton pulp. And performing primary coarse grinding on the pulp subjected to chemical impregnation, and performing supplementary bleaching, wherein the dosage of hydrogen peroxide is 30 kg/ton pulp, the dosage of caustic soda is 20 kg/ton pulp, the dosage of sodium silicate is 20 kg/ton pulp, the dosage of DTPA is 5 kg/ton pulp, and the dosage of magnesium sulfate is 2 kg/ton pulp in the bleaching process. The bleached pulp is finely ground to prepare chemi-mechanical pulp after grinding, the chemi-mechanical pulp is respectively subjected to latency, washing, screening and purification to form paper pulp for paper pulp performance test and comparison of power consumption of ton pulp, and the comparison result is shown in table 1:
table 1: performance comparison of poplar APMP chemi-mechanical pulp
| Serial number | Formulation of | Freeness (ml) | Whiteness (ISO%) | Yield of finished fiber (%) | Tensile index (N.m/g) | Tear index (N.mm) 2 /g） | Folding endurance (times) | Ton pulp power consumption (KWH) |
| 1 | Blank space | 307 | 81.7 | 87.3 | 29.4 | 4.53 | 37 | 1217 |
| 2 | A | 288 | 84.4 | 88.9 | 30.6 | 4.78 | 41 | 1107 |
| 3 | B | 285 | 84.1 | 88.6 | 30.7 | 4.86 | 40 | 1096 |
| 4 | C | 281 | 83.9 | 89.4 | 30.2 | 4.67 | 40 | 1085 |
Example 2: eucalyptus APMP
Storing eucalyptus pieces cut according to standard specifications, and respectively adding the biological enzyme preparations of the formulas A, B and C into clear water according to the ratio of 1: diluting at 100 proportion. The diluted enzyme preparation solution is uniformly sprayed on wood chips of a feeding bin, 0.2 kg of compound biological enzyme preparation is used for pulp per ton, the wood chips sprayed with the enzyme preparation solution react in the bin for 48 hours for washing, steaming and screw extrusion, the pulp after screw extrusion is chemically impregnated, the dosage of hydrogen peroxide in the impregnating process is 20 kg/ton pulp, the dosage of caustic soda is 30 kg/ton pulp, the dosage of sodium silicate in the impregnating process is 30 kg/ton pulp, the dosage of DTPA in the impregnating process is 5 kg/ton pulp, and the dosage of magnesium sulfate in the impregnating process is 2 kg/ton pulp. And performing primary coarse grinding on the pulp subjected to chemical impregnation, and performing supplementary bleaching, wherein the dosage of hydrogen peroxide is 30 kg/ton pulp, the dosage of caustic soda is 20 kg/ton pulp, the dosage of sodium silicate is 20 kg/ton pulp, the dosage of DTPA is 5 kg/ton pulp, and the dosage of magnesium sulfate is 2 kg/ton pulp in the bleaching process. And (3) fine grinding the bleached pulp, grinding the pulp to obtain chemical-mechanical pulp, and performing latency, washing, screening and purifying on the chemical-mechanical pulp to obtain paper pulp for paper pulp performance testing and comparing the power consumption per ton of the pulp, wherein the comparison result is shown in a table 2.
Table 2: performance comparison of eucalyptus APMP chemi-mechanical pulp
| Serial number | Formulation of | Freeness (ml) | Whiteness (ISO%) | Yield of pulped fiber (%) | Tensile index (N.m/g) | Tear index (N.mm) 2 /g） | Folding endurance (times) | Ton pulp power consumption (KWH) |
| 1 | Blank space | 302 | 80.6 | 88.3 | 27.8 | 4.34 | 35 | 1245 |
| 2 | A | 291 | 83.4 | 89.7 | 30.2 | 4.68 | 39 | 1127 |
| 3 | B | 288 | 83.2 | 89.6 | 30.5 | 4.76 | 38 | 1115 |
| 4 | C | 285 | 82.7 | 90.3 | 30.1 | 4.69 | 37 | 1102 |
Example 3: mixed wood chip (30% eucalyptus and 70% poplar) APMP
Storing eucalyptus pieces cut according to standard specifications, and respectively adding the biological enzyme preparations of the formulas A, B and C into clear water according to the ratio of 1: diluting at 100 proportion. The diluted enzyme preparation solution is uniformly sprayed on wood chips of a feeding bin, 0.2 kg of compound biological enzyme preparation is used for pulp per ton, the wood chips sprayed with the enzyme preparation solution react in the bin for 48 hours, the wood chips are washed, steamed and spirally extruded, the pulp after the spiral extrusion is chemically impregnated, the dosage of hydrogen peroxide in the impregnating process is 20 kg/ton pulp, the dosage of caustic soda is 30 kg/ton pulp, the dosage of sodium silicate is 30 kg/ton pulp, the dosage of DTPA is 5 kg/ton pulp, and the dosage of magnesium sulfate is 2 kg/ton pulp. And performing primary coarse grinding on the pulp subjected to chemical impregnation, and performing supplementary bleaching, wherein the dosage of hydrogen peroxide is 30 kg/ton pulp, the dosage of caustic soda is 20 kg/ton pulp, the dosage of sodium silicate is 20 kg/ton pulp, the dosage of DTPA is 5 kg/ton pulp, and the dosage of magnesium sulfate is 2 kg/ton pulp in the bleaching process. And (3) finely grinding the bleached pulp, preparing chemical-mechanical pulp after grinding, and respectively performing latency, washing, screening and purification on the chemical-mechanical pulp to obtain paper pulp for paper pulp performance test and comparing the power consumption of each ton of pulp, wherein the comparison result is shown in table 3.
Table 3: chemical-mechanical pulp performance comparison of APMP of mixed wood chips (30% eucalyptus and 70% poplar)
| Serial number | Formulation of | Freeness (ml) | Whiteness (ISO%) | Yield of finished fiber (%) | Tensile index (N.m/g) | Tear index (N.mm) 2 /g） | Folding endurance (second) | Ton pulp power consumption (KWH) |
| 1 | Blank space | 294 | 80.9 | 89.3 | 28.8 | 4.43 | 38 | 1225 |
| 2 | A | 286 | 83.9 | 90.7 | 31.2 | 4.74 | 41 | 1107 |
| 3 | B | 280 | 83.7 | 90.6 | 31.4 | 4.85 | 42 | 1098 |
| 4 | C | 275 | 83.4 | 91.3 | 31.2 | 4.89 | 42 | 1087 |
Combining the above 3 embodiments can result in: the chemi-mechanical pulp with the pulp freeness of about 290ml is obtained from the same raw materials, the whiteness value (ISO) of the pulp added with the compound bio-enzyme preparation is increased by about 3 percent compared with the pulp without the bio-enzyme, the fiber yield is improved by 1 percent, the power consumption per ton pulp is reduced by about 110KWH, the tensile index of the pulp is improved by 2.5 N.m/g, and the tearing index of the pulp is improved by 0.3 N.mm 2 The folding strength of the paper pulp is increased by 4 times, and the strength of the paper pulp is improved. The formula C has the best effect on saving energy consumption, and the formula A has the best effect on improving the whiteness of paper pulp.
The invention has various embodiments, and all technical solutions formed by adopting equivalent transformation or equivalent transformation are within the protection scope of the invention.
Claims (5)
1. A compound biological enzyme preparation: the biological enzyme preparation comprises the following components in percentage by mass,
30-35% of endo-cellulase;
20-25% of exo-cellulase;
2-5% of sodium chloride;
1-3% of potassium sorbate;
0-12% of deionized water is not 0;
the preparation also comprises 15-20% of xylanase; 10-15% of pectinase;
the preparation also comprises 10-15% of laccase;
the enzyme activity of the endo-cellulase is 15000-20000u/ml; the enzyme activity of the exo-cellulase is 15000 to 20000u/ml;
the enzyme activity of the xylanase is 20000-25000u/ml, and the enzyme activity of the pectinase is 20000-25000u/ml;
the enzyme activity of the laccase is 30000-35000u/ml.
2. The preparation method of the compound biological enzyme preparation as claimed in claim 1, which is characterized in that: the preparation method comprises the following steps of firstly adding deionized water into a stirring tank, adding sodium chloride, pectinase and laccase into the stirring tank according to a proportion, stirring, then continuously stirring endo-cellulase, exo-cellulase and xylanase, and finally adding potassium sorbate according to a proportion, and stirring to obtain the compound bio-enzyme preparation.
3. The use of a formulated bio-enzyme preparation according to claim 1, wherein: the biological enzyme preparation is used for preparing chemi-mechanical pulp.
4. The use of a formulated bio-enzyme preparation according to claim 3, wherein: the preparation of the chemi-mechanical pulp by the biological enzyme preparation comprises the following steps:
s1, preparing materials: screening the wood chips to a wood chip bin;
s2, biological enzyme pretreatment: pretreating wood chips entering a wood chip bin, wherein the condition of biological enzyme pretreatment is that the pH is 6-8, the temperature is 30-70 ℃, the dosage of a biological enzyme preparation is 200 g/absolute dry pulp, and the reaction time is more than 48 hours; diluting the compound biological enzyme preparation by 100 times with clear water, uniformly spraying the diluted biological enzyme preparation on wood chips entering a wood chip bin, and storing the wood chips sprayed with the biological enzyme in the wood chip bin for more than 48 hours;
s3, washing: washing the wood chips treated in the step S2 and presteaming under normal pressure;
s4, screw extrusion: carrying out screw extrusion on the wood chips treated in the step S3 to obtain extruded pulp;
s5, chemical impregnation: taking alkaline hydrogen peroxide solution as impregnation liquid medicine, and chemically impregnating the extruded slurry;
s6, first-stage coarse grinding: carrying out normal-pressure grinding on the slurry of the S5, wherein the mass concentration of the slurry is 20-25%;
s7, bleaching: performing supplementary bleaching on the slurry of the S6; the solvent of the bleached liquid medicine is water, the solutes comprise hydrogen peroxide, sodium hydroxide, sodium silicate and DTPA, and the ratio of the dosage of each solute to the oven-dried mass of the pulp is as follows: 3 to 4 percent of hydrogen peroxide, 2 to 4 percent of sodium hydroxide, 3.0 percent of sodium silicate and 0.5 percent of DTPA;
s8, two-stage fine grinding: carrying out normal-pressure pulping on the pulp obtained in the step S7 to prepare chemi-mechanical pulp, and carrying out latency elimination, washing, screening and purification on the chemi-mechanical pulp to obtain paper pulp for testing the performance of the paper pulp, wherein the mass concentration of the pulp is 20-25%; the latency elimination conditions are as follows: the latency elimination temperature is above 80 ℃, and the latency elimination time is 10-30min.
5. The use of the reconstituted biological enzyme preparation according to claim 4, characterized in that: the temperature of the normal-pressure presteaming in the S3 is 90-105 ℃, and the time is 10-20min; in the S4, double-screw extrusion is adopted for the screw extrusion, and the extrusion temperature is 45-60 ℃; the solvent for soaking the liquid medicine in the S5 is water, the solutes comprise hydrogen peroxide, sodium hydroxide, sodium silicate and DTPA, and the ratio of the dosage of each solute to the oven-dried mass of the slurry is as follows: the content of hydrogen peroxide is 2-3%, the content of sodium hydroxide is 2-3%, the content of sodium silicate is 1.0%, the content of DTPA is 0.5%, the solute also comprises magnesium sulfate, and the content of magnesium sulfate is 0.1-0.2% of the oven-dried mass of the slurry; in S5, the mass concentration of the slurry in the dipping liquid medicine is 20-25%, the dipping time is 40-60min, and the dipping temperature is 70-90 ℃; the solute in the S7 also comprises magnesium sulfate, and the dosage of the magnesium sulfate is 0.1% -0.2% of the oven-dried mass of the slurry.
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