CN112410311A - Papermaking biological enzyme composition and preparation thereof - Google Patents

Papermaking biological enzyme composition and preparation thereof Download PDF

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Publication number
CN112410311A
CN112410311A CN202011286108.3A CN202011286108A CN112410311A CN 112410311 A CN112410311 A CN 112410311A CN 202011286108 A CN202011286108 A CN 202011286108A CN 112410311 A CN112410311 A CN 112410311A
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amylase
cellulase
parts
treatment
biological enzyme
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CN112410311B (en
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孙新富
武运佐
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Suzhou Aisitengte Biotechnology Co ltd
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Suzhou Aisitengte Biotechnology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y110/00—Oxidoreductases acting on diphenols and related substances as donors (1.10)
    • C12Y110/03—Oxidoreductases acting on diphenols and related substances as donors (1.10) with an oxygen as acceptor (1.10.3)
    • C12Y110/03002—Laccase (1.10.3.2)
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/02—Working-up waste paper
    • D21C5/025—De-inking
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/02—Working-up waste paper
    • D21C5/025—De-inking
    • D21C5/027—Chemicals therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/64—Paper recycling

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  • Paper (AREA)

Abstract

The invention relates to the technical field of waste paper stickies and printing ink treatment, in particular to a papermaking biological enzyme composition and a preparation thereof, wherein the biological enzyme composition comprises the following components in parts by weight: 5-10 parts of amylase, 2-5 parts of laccase, 5-10 parts of modified cellulase, 6-10 parts of xylanase and 1-3 parts of lipase.

Description

Papermaking biological enzyme composition and preparation thereof
Technical Field
The invention relates to the technical field of waste paper stickies and printing ink treatment, in particular to a papermaking biological enzyme composition and a preparation thereof.
Background
Along with economic development and industrial progress, various papers are demanded more and more strongly, but the cost of raw wood pulp used as a raw material is higher and higher, so the significance of energy conservation and consumption reduction is increasingly profound to the papermaking industry, the problem is well solved by improving the recycling rate of the waste paper, however, various impurities with complex components and various types are entrained in the waste paper and enter a circulation system of a paper machine, and a series of problems are brought to the recycling process of the waste paper, wherein the influence caused by stickies and printing ink is the most prominent. The increase of stickies can pollute the paper-making net, the blanket, the drying cylinder and the calender roll, simultaneously can cause paper defects and paper breakage, and finally can influence the subsequent processing and printing operation.
Currently, the most common sticker control technologies can be summarized into three broad categories according to their basic principles: mechanical physical methods, chemical methods and biological methods.
The mechanical (physical) method is mainly used for controlling large stickies and part of fine stickies with larger size, is a main method for controlling stickies in a paper mill using waste paper as a main raw material, and has been widely used in actual production. The mechanical and physical method is the most common economic and efficient method for controlling stickies in the production process, but the effect of removing stickies by the method depends on the design of the whole production process and the performance of production equipment, and the quality of the design of the production process and the performance of the equipment determine the removal efficiency of the stickies.
The chemical control method is to dissolve, passivate, disperse or maintain the dispersed state of the stickies in the waste paper pulp or white water circulation system by adding one or more stickies control agents, or to make the stickies adsorb or combine on the fibers or the wet paper sheet and finally remain in the paper sheet so as to leave the water circulation system. The control target objects of the chemical control method are mainly fine stickies and secondary stickies which are difficult to completely remove by using a physical method. These glues have in common the fact that the particle size is generally less than a few tens of microns, even a considerable fraction of particles belonging to the colloidal category having a size of less than 1 μm being present. However, the chemical control method causes serious fiber loss, the strength of the treated fiber is also reduced, and the use of chemical agents seriously pollutes the environment.
The use of bio-enzyme preparations in the pulp and paper industry has been experienced for many years, primarily for controlling microbial deposits to enhance bleaching and cleaning of paper machine systems, but the use of bio-enzyme preparations to control stickies in waste pulp and white water recycling systems has recently begun, and specific bio-enzyme preparations have allowed for size reduction and effective stickies control, and have also reduced stickies viscosity and prevented flocculation of stickies particles. Studies of the chemical composition of the glue show that: most adhesives contain a large number of ester linkages which link the basic structural components of the adhesive together. The greatest advantage of the bio-enzyme control method is that once an ester bond is broken, the basic components of the adhesive are difficult to repolymerize in the system, and the bio-enzyme can be naturally degraded and does not pollute the environment.
The Chinese patent application CN104694515A discloses a biological enzyme composition for synchronously treating cultural paper ink and stickies and an application thereof, wherein the biological enzyme composition consists of a plurality of biological enzymes and auxiliaries, the biological enzyme composition synchronously treats cultural paper deinking and stickies removal through the synergistic effect of the biological enzymes and the auxiliaries in the biological enzyme composition at a proper temperature and pH, the papermaking process steps are simplified, the treatment efficiency is high, the flotation whiteness improvement value is averagely 4.8% ISO, the flotation residual ink removal rate is averagely 41.4%, the flotation large stickies removal rate is averagely 76.3%, the micro flotation stickies removal rate is averagely 66.1%, the deinking effect and stickies removal effect are good, the paper pulp quality can be improved, and the service life of a paper machine is prolonged.
Chinese patent application CN106318930A discloses a method for removing stickies from waste paper pulp by using a complex enzyme preparation comprising high temperature esterase TTEST, amylase, pectinase, xylanase, glycerol, polyethylene glycol and water.
Chinese patent application CN103669066A discloses a method for improving the removal effect of stickies in waste cardboard paper, which comprises the following steps: adding waste boxboard and water into a pulper, simultaneously adding 0.01-0.03% of composite enzyme consisting of lipase, cellulase and xylanase in weight of the waste boxboard and 0.01-0.03% of flotation collector in weight of the waste boxboard, and performing disintegration for 15-25min at 35-65 ℃ to obtain paper pulp with pulp concentration of 10-18%; then adding the paper pulp into a flotation tank for flotation; finally, the paper pulp is further diluted to the pulp concentration of 0.1-0.2% and washed by a 120-mesh copper net. Through the mode, the method disclosed by the invention enables the removal rate of the stickies in the waste cardboard paper to be as high as 60.1-64%.
However, the stickies and the ink compositions in different types of waste paper pulp are different, most of the methods in the prior art can only effectively treat one type of waste paper pulp, and two problems of deinking and stickies treatment are often separately studied, so that the treatment efficiency is low.
Therefore, it is necessary to develop a bio-enzyme composition and a preparation thereof that can solve the above-mentioned technical problems.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a papermaking biological enzyme composition and a preparation thereof, wherein the papermaking biological enzyme composition has wide application range, good effect of treating stickies and deinking and high treatment efficiency.
The invention is realized by the following technical scheme:
a biological enzyme composition comprises the following components in parts by weight: 5-10 parts of amylase, 2-5 parts of laccase, 5-10 parts of modified cellulase, 6-10 parts of xylanase and 1-3 parts of lipase.
Preferably, the amylase is a mixture of alpha-amylase and beta-amylase, and the mass ratio of the alpha-amylase to the beta-amylase is 2-4: 1.
Preferably, the preparation process of the modified cellulase comprises the following steps: dissolving cellulase in a buffer solution to obtain a cellulase solution, sequentially adding N-hydroxysuccinimide and mixed amine, dialyzing and removing unreacted mixed amine and N-hydroxysuccinimide after the reaction is finished, and freeze-drying the residual solution to obtain the modified cellulase.
More preferably, the mixed amine is a mixture of diethylenetriamine and 1, 6-hexanediamine, and the mass ratio of the diethylenetriamine to the 1, 6-hexanediamine is 1.5-3: 1.
More preferably, the mass fraction of cellulase in the cellulase solution is 2-4%.
More preferably, the mass ratio of the N-hydroxysuccinimide to the cellulase solution is 0.002-0.005: 1.
More preferably, the mass ratio of the mixed amine to the cellulase solution is 0.01-0.03: 1.
More preferably, the preparation process of the modified cellulase comprises the following steps: dissolving cellulase in phosphate buffer solution with pH of 7.2 to obtain cellulase solution, adding N-hydroxysuccinimide, stirring for 3-5min, slowly adding mixed amine, reacting for 3-6h at 30-40 ℃ under stirring, dialyzing unreacted mixed amine and N-hydroxysuccinimide to remove, and freeze-drying the residual solution to obtain the modified cellulase.
More preferably, the enzyme activity of the cellulase is 1000-3000U/g.
Preferably, the enzyme activity of the amylase is 4000-6000U/g, the enzyme activity of the laccase is 20000-30000U/g, the enzyme activity of the xylanase is 10000-20000U/g, and the enzyme activity of the lipase is 10000-20000U/g.
The invention also relates to a biological enzyme preparation which comprises the biological enzyme composition.
Preferably, the biological enzyme preparation further comprises a surfactant, an organic solvent, a stabilizer and water.
More preferably, the biological enzyme preparation further comprises 10-20 parts by weight of a surfactant, 30-40 parts by weight of an organic solvent, 5-10 parts by weight of a stabilizer and 15-25 parts by weight of water.
More preferably, the surfactant comprises at least one of fatty alcohol-polyoxyethylene ether, alkylphenol ethoxylate and octylphenol-polyoxyethylene ether.
More preferably, the organic solvent includes at least one of ethanol, n-butanol, and polyethylene glycol.
More preferably, the organic solvent is a mixed solvent of ethanol, n-butanol and polyethylene glycol, and the volume ratio of the three is 2-4:1-3: 1.
More preferably, the stabilizer includes at least one of glycerin and sorbitol.
The invention also relates to a method for simultaneously treating the adhesive and the printing ink by adopting the biological enzyme preparation, which comprises the following steps:
(1) shredding waste paper, adding a surfactant, an organic solvent, a stabilizer and water, uniformly mixing, and then sequentially adding amylase, laccase, modified cellulase, xylanase and lipase under the stirring condition to obtain a mixture A; the mass of the biological enzyme preparation is 0.03-0.05% of the mass of the waste paper;
(2) the mixture A is pulped in a high-concentration pulper to obtain a substance B;
(3) pouring the substance B into a fine screening device, and pouring the substance B into a flotation tank after the fine screening is finished to obtain a substance C;
(4) the material C was washed in a washer and passed through a 120 mesh screen with water until only fiber remained.
Preferably, the amylase treatment is carried out for 10-15min, the laccase treatment is carried out for 5-8min, the modified cellulase treatment is carried out for 10-20min, the xylanase treatment is carried out for 8-15min, and the lipase treatment is carried out for 3-5min in sequence in the step (1), so as to obtain a mixture A.
More preferably, the method comprises the steps of:
(1) shredding waste paper, adding surfactant, organic solvent, stabilizer and water, mixing uniformly, adjusting temperature to 40-60 deg.C, adjusting pH to 6.0-8.0, and then sequentially adding amylase for treatment for 10-15min, laccase for treatment for 5-8min, modified cellulase for treatment for 10-20min, xylanase for treatment for 8-15min, and lipase for treatment for 3-5min under stirring condition; obtaining a mixture A; the mass of the biological enzyme preparation is 0.03-0.05% of the mass of the waste paper.
(2) The mixture A is pulped in a high-concentration pulper for 20-30min, the temperature of the pulping is 40-60 ℃, the pH of the pulping is 6.0-8.0, and the concentration of the pulping is 15-18%, so that a substance B is obtained;
(3) pouring the substance B into a fine screen device, adjusting the concentration of the slurry to 1-3% after the fine screen is finished, and then pouring the slurry into a flotation tank, wherein the flotation time is 5-8min, and the flotation temperature is 40-60 ℃, so as to obtain a substance C;
(4) the material C was washed in a washer and passed through a 120 mesh screen with water until only fiber remained.
The invention has the beneficial effects that:
the invention optimizes the composition and the dosage ratio of the biological enzyme composition, and obviously improves the removal effect of stickies and printing ink.
The invention adopts modified cellulase to replace cellulase, optimizes the preparation process thereof, for example, adopts N-hydroxysuccinimide and mixed amine to modify the cellulase, optimizes the composition of the mixed amine and improves the removal effect of stickies and printing ink.
The biological enzyme preparation of the invention selects specific surfactant, organic solvent and stabilizer, which is beneficial to improving the stability of the preparation, ensuring the full contact of the biological enzyme and paper pulp and improving the treatment efficiency of the biological enzyme.
The invention optimizes the treatment sequence of the biological enzyme in the treatment process, and obviously improves the removal effect of the stickies and the printing ink.
Detailed Description
The invention will be further described with reference to specific embodiments, and the advantages and features of the invention will become apparent as the description proceeds. These examples are illustrative only and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
Example 1
A biological enzyme preparation comprises the following components in parts by weight: 5 parts of amylase, 2 parts of laccase, 5 parts of modified cellulase, 6 parts of xylanase, 1 part of lipase, 10 parts of fatty alcohol-polyoxyethylene ether, 30 parts of organic solvent, 5 parts of glycerol and 15 parts of water; the enzyme activity of the amylase is 4000U/g, the enzyme activity of the laccase is 20000U/g, the enzyme activity of the xylanase is 10000U/g, and the enzyme activity of the lipase is 10000U/g.
The amylase is a mixture of alpha-amylase and beta-amylase, and the mass ratio of the alpha-amylase to the beta-amylase is 2: 1.
The organic solvent is a mixed solvent of ethanol, n-butyl alcohol and polyethylene glycol, and the volume ratio of the ethanol to the n-butyl alcohol to the polyethylene glycol is 2:1: 1.
The preparation process of the modified cellulase comprises the following steps: dissolving cellulase (the enzyme activity is 1000U/g) in phosphate buffer solution with the pH value of 7.2 to obtain cellulase solution, wherein the mass fraction of the cellulase in the cellulase solution is 2%, adding 0.2% of N-hydroxysuccinimide, stirring for 3min, slowly adding 1% of mixed amine, wherein the mixed amine is a mixture of diethylenetriamine and 1, 6-hexanediamine, the mass ratio of the diethylenetriamine to the 1, 6-hexanediamine is 1.5:1, reacting for 3h under the stirring condition at 30 ℃, dialyzing and removing the unreacted mixed amine and the N-hydroxysuccinimide, and freeze-drying the residual solution to obtain the modified cellulase.
A method for simultaneously treating cultural paper stickies and printing ink by using the biological enzyme preparation comprises the following steps:
(1) shredding waste paper, adding fatty alcohol-polyoxyethylene ether, an organic solvent, glycerol and water, uniformly mixing, adjusting the temperature to 40 ℃, adjusting the pH to 6.0, and then sequentially adding amylase for treatment for 10min, laccase for treatment for 5min, modified cellulase for treatment for 10min, xylanase for treatment for 8min and lipase for treatment for 3min under the stirring condition; obtaining a mixture A; the mass of the biological enzyme preparation is 0.03 percent of the mass of the waste paper.
(2) The mixture A is pulped in a high-concentration pulper for 20min, the temperature of the pulping is 40 ℃, the pH of the pulping is 6.0, and the concentration of the pulping is 15 percent, so that a substance B is obtained;
(3) pouring the substance B into a fine screening device, adjusting the concentration of the slurry to 1% after the fine screening is finished, and then pouring the slurry into a flotation tank, wherein the flotation time is 5min, and the flotation temperature is 40 ℃, so as to obtain a substance C;
(4) the material C was washed in a washer and passed through a 120 mesh screen with water until only fiber remained.
Example 2
A biological enzyme preparation comprises the following components in parts by weight: 10 parts of amylase, 5 parts of laccase, 10 parts of modified cellulase, 10 parts of xylanase, 3 parts of lipase, 20 parts of alkylphenol polyoxyethylene, 40 parts of organic solvent, 10 parts of sorbitol and 25 parts of water; the enzyme activity of the amylase is 6000U/g, the enzyme activity of the laccase is 30000U/g, the enzyme activity of the xylanase is 20000U/g, and the enzyme activity of the lipase is 20000U/g.
The amylase is a mixture of alpha-amylase and beta-amylase, and the mass ratio of the alpha-amylase to the beta-amylase is 4: 1.
The organic solvent is a mixed solvent of ethanol, n-butyl alcohol and polyethylene glycol, and the volume ratio of the ethanol to the n-butyl alcohol to the polyethylene glycol is 4:3: 1.
The preparation process of the modified cellulase comprises the following steps: dissolving cellulase (the enzyme activity is 3000U/g) in phosphate buffer solution with the pH value of 7.2 to obtain cellulase solution, wherein the mass fraction of the cellulase in the cellulase solution is 4%, adding 0.5% of N-hydroxysuccinimide, stirring for 5min, slowly adding 3% of mixed amine, the mixed amine is a mixture of diethylenetriamine and 1, 6-hexanediamine, the mass ratio of the diethylenetriamine to the 1, 6-hexanediamine is 3:1, reacting for 6h under the stirring condition at 40 ℃, dialyzing and removing the unreacted mixed amine and the N-hydroxysuccinimide, and freezing and drying the residual solution to obtain the modified cellulase.
A method for simultaneously treating used cardboard paper stickies and ink with the biological enzyme preparation, comprising the steps of:
(1) shredding waste paper, adding alkylphenol ethoxylates, an organic solvent, sorbitol and water, uniformly mixing, adjusting the temperature to 60 ℃, adjusting the pH to 8.0, and then sequentially adding amylase for treatment for 15min, laccase for treatment for 8min, modified cellulase for treatment for 20min, xylanase for treatment for 15min and lipase for treatment for 5min under the stirring condition; obtaining a mixture A; the mass of the biological enzyme preparation is 0.05 percent of the mass of the waste paper.
(2) The mixture A is pulped in a high-concentration pulper for 30min, the temperature of the pulping is 60 ℃, the pH value of the pulping is 8.0, and the concentration of the pulping is 18 percent, so that a substance B is obtained;
(3) pouring the substance B into a fine screen device, adjusting the concentration of the slurry to 3% after the fine screen is finished, and then pouring the slurry into a flotation tank, wherein the flotation time is 8min, and the flotation temperature is 60 ℃, so as to obtain a substance C;
(4) the material C was washed in a washer and passed through a 120 mesh screen with water until only fiber remained.
Example 3
A biological enzyme preparation comprises the following components in parts by weight: 8 parts of amylase, 3 parts of laccase, 7 parts of modified cellulase, 8 parts of xylanase, 2 parts of lipase, 15 parts of octyl phenol polyoxyethylene ether, 35 parts of organic solvent, 9 parts of sorbitol and 20 parts of water; the enzyme activity of the amylase is 5000U/g, the enzyme activity of the laccase is 25000U/g, the enzyme activity of the xylanase is 15000U/g, and the enzyme activity of the lipase is 15000U/g.
The amylase is a mixture of alpha-amylase and beta-amylase, and the mass ratio of the alpha-amylase to the beta-amylase is 3: 1.
The organic solvent is a mixed solvent of ethanol, n-butyl alcohol and polyethylene glycol, and the volume ratio of the ethanol to the n-butyl alcohol to the polyethylene glycol is 3:2: 1.
The preparation process of the modified cellulase comprises the following steps: dissolving cellulase (the enzyme activity is 2000U/g) in phosphate buffer solution with the pH value of 7.2 to obtain cellulase solution, wherein the mass fraction of the cellulase in the cellulase solution is 3%, adding 0.4% of N-hydroxysuccinimide, stirring for 4min, slowly adding 2% of mixed amine, the mixed amine is a mixture of diethylenetriamine and 1, 6-hexanediamine, the mass ratio of the diethylenetriamine to the 1, 6-hexanediamine is 2:1, reacting for 5h under the stirring condition at 35 ℃, dialyzing and removing the unreacted mixed amine and the N-hydroxysuccinimide, and freezing and drying the residual solution to obtain the modified cellulase.
A method for simultaneously treating cultural paper stickies and printing ink by using the biological enzyme preparation comprises the following steps:
(1) shredding waste paper, adding octyl phenol polyoxyethylene ether, an organic solvent, sorbitol and water, uniformly mixing, adjusting the temperature to 50 ℃, adjusting the pH to 7.0, and then sequentially adding amylase for treatment for 12min, laccase for treatment for 7min, modified cellulase for treatment for 15min, xylanase for treatment for 10min and lipase for treatment for 4min under the stirring condition; obtaining a mixture A; the mass of the biological enzyme preparation is 0.04 percent of the mass of the waste paper.
(2) The mixture A is pulped in a high-concentration pulper for 25min, the temperature of the pulping is 50 ℃, the pH value of the pulping is 7.0, and the concentration of the pulping is 16 percent, so that a substance B is obtained;
(3) pouring the substance B into a fine screening device, adjusting the concentration of the slurry to 2% after the fine screening is finished, and then pouring the slurry into a flotation tank, wherein the flotation time is 7min, and the flotation temperature is 50 ℃, so as to obtain a substance C;
(4) the material C was washed in a washer and passed through a 120 mesh screen with water until only fiber remained.
Comparative example 1
The difference from example 3 is only that the composition of the bio-enzyme composition is different, 2 parts of lipase is replaced by 2 parts of pectinase, and the rest conditions are the same.
Comparative example 2
The only difference from example 3 was that the modified cellulase was replaced with an equal amount of cellulase and the conditions were the same.
Comparative example 3
The difference from example 3 is only that the mixed amine was replaced with equal amount of tetraethylenepentamine during the preparation of the modified cellulase, and the other conditions were the same.
Comparative example 4
The difference from example 3 is only that in the preparation process of the modified cellulase, the mixed amine is replaced by the same amount of diethylenetriamine, and the rest conditions are the same.
Comparative example 5
The only difference from example 3 is that the mixed amine was replaced with the same amount of 1, 6-hexanediamine during the preparation of the modified cellulase, and the other conditions were the same.
Comparative example 6
The difference from the example 3 is that the total volume of the organic solvent is unchanged, the composition is different, and the rest conditions are the same, specifically as follows: the organic solvent is a mixed solvent of 1, 2-propylene glycol, 1, 4-butanediol and ethanol, and the volume ratio of the three is 2:1: 1.
Comparative example 7
The organic solvent is a mixed solvent of ethanol, n-butyl alcohol and polyethylene glycol, the volume ratio of the ethanol to the n-butyl alcohol to the polyethylene glycol is 1:2:3, and the rest conditions are the same.
Comparative example 8
The difference from the example 3 is only that the treatment sequence of the biological enzyme is different in the process of treating the waste paper by using the biological enzyme preparation, and the rest conditions are the same, and the specific conditions are as follows:
(1) shredding waste paper, adding octyl phenol polyoxyethylene ether, an organic solvent, sorbitol and water, uniformly mixing, adjusting the temperature to 50 ℃, adjusting the pH to 7.0, and then sequentially adding amylase for treatment for 12min, lipase for treatment for 4min, xylanase for treatment for 10min, modified cellulase for treatment for 15min and laccase for treatment for 7min under the stirring condition; obtaining a mixture A; the mass of the biological enzyme preparation is 0.04 percent of the mass of the waste paper.
Test example 1
1. Measurement of whiteness and residual ink: the slurry before and after flotation was collected and sheeted on a standard sheet former (basis weight 200 g/m)2) Measuring the whiteness and residual ink content, and measuring the whiteness and residual ink concentration (ERIC) by Tech2nidyne Color Touch [ Eric950 ]]And (4) measuring.
2. Determination of large stickies: pulp before and after flotation with the absolute dry content of 2g is measured, screened in a Pulmac-MasterScreen sticky matter screening instrument after defibering, dyed, tabletted, scanned and analyzed by software carried by the instrument.
3. Measuring the fine adhesive substance: 200mL of slurry before and after flotation is measured, centrifugal separation is carried out for 15min at the rotating speed of 5000rpm, supernatant fluid is taken, suction filtration is carried out on an ultrafiltration membrane of 0.22um, and then weighing is carried out after constant weight.
The results are shown in tables 1 and 2.
Wherein the blank group is treated without adding biological enzyme preparation, and the rest conditions are the same as those in the example 3, and the details are as follows:
a method for simultaneously treating cultural paper stickies and printing ink by using the biological enzyme preparation comprises the following steps:
(1) shredding waste paper, adding water, mixing well, adjusting temperature to 50 deg.C, adjusting pH to 7.0, and then treating for 48min under stirring; obtaining a mixture A; the mass of the water was 0.04% of the mass of the waste paper.
(2) The mixture A is pulped in a high-concentration pulper for 25min, the temperature of the pulping is 50 ℃, the pH value of the pulping is 7.0, and the concentration of the pulping is 16 percent, so that a substance B is obtained;
(3) pouring the substance B into a fine screening device, adjusting the concentration of the slurry to 2% after the fine screening is finished, and then pouring the slurry into a flotation tank, wherein the flotation time is 7min, and the flotation temperature is 50 ℃, so as to obtain a substance C;
(4) the material C was washed in a washer and passed through a 120 mesh screen with water until only fiber remained.
And testing results before flotation, namely testing results of materials before the materials enter the flotation tank after the material B is finely screened.
The test result after flotation is the material test result of substance C.
TABLE 1 test results for whiteness, residual ink level, high stickies content and fine stickies content before flotation
Figure BDA0002782465110000081
Examples 1 and 3 are the same as the raw materials for each comparative example.
Table 2 test results of whiteness, residual ink amount, large stickies content and fine stickies content after flotation
Figure BDA0002782465110000082
Figure BDA0002782465110000091
The above detailed description is specific to one possible embodiment of the present invention, and the embodiment is not intended to limit the scope of the present invention, and all equivalent implementations or modifications without departing from the scope of the present invention should be included in the technical scope of the present invention.

Claims (10)

1. The biological enzyme composition is characterized by comprising the following components in parts by weight: 5-10 parts of amylase, 2-5 parts of laccase, 5-10 parts of modified cellulase, 6-10 parts of xylanase and 1-3 parts of lipase.
2. The biological enzyme composition according to claim 1, wherein the amylase is a mixture of alpha-amylase and beta-amylase, and the mass ratio of the alpha-amylase to the beta-amylase is 2-4: 1.
3. The biological enzyme composition according to claim 1, wherein the preparation process of the modified cellulase comprises the following steps: dissolving cellulase in a buffer solution to obtain a cellulase solution, sequentially adding N-hydroxysuccinimide and mixed amine, dialyzing and removing unreacted mixed amine and N-hydroxysuccinimide after the reaction is finished, and freeze-drying the residual solution to obtain the modified cellulase.
4. The biological enzyme composition according to claim 3, wherein the mixed amine is a mixture of diethylenetriamine and 1, 6-hexanediamine at a mass ratio of 1.5-3: 1.
5. The biological enzyme composition according to claim 3, characterized in that the mass fraction of cellulase in the cellulase solution is 2-4%; the mass ratio of the N-hydroxysuccinimide to the cellulase solution is 0.002-0.005: 1; the mass ratio of the mixed amine to the cellulase solution is 0.01-0.03: 1.
6. The bio-enzyme composition as claimed in claim 1, wherein the enzyme activity of said amylase is 4000-6000U/g, the enzyme activity of said laccase is 20000-30000U/g, the enzyme activity of said xylanase is 10000-20000U/g, and the enzyme activity of said lipase is 10000-20000U/g.
7. A bio-enzyme preparation comprising the bio-enzyme composition according to any one of claims 1 to 6.
8. The bio-enzyme formulation according to claim 7, further comprising a surfactant, an organic solvent, a stabilizer and water.
9. A method of simultaneously treating stickies and inks with the bio-enzyme preparation of claim 8, comprising the steps of:
(1) shredding waste paper, adding a surfactant, an organic solvent, a stabilizer and water, uniformly mixing, and then sequentially adding amylase, laccase, modified cellulase, xylanase and lipase under the stirring condition to obtain a mixture A; the mass of the biological enzyme preparation is 0.03-0.05% of the mass of the waste paper;
(2) the mixture A is pulped in a high-concentration pulper to obtain a substance B;
(3) pouring the substance B into a fine screening device, and pouring the substance B into a flotation tank after the fine screening is finished to obtain a substance C;
(4) the material C was washed in a washer and passed through a 120 mesh screen with water until only fiber remained.
10. The method of claim 9, wherein the amylase treatment for 10-15min, the laccase treatment for 5-8min, the modified cellulase treatment for 10-20min, the xylanase treatment for 8-15min, and the lipase treatment for 3-5min are sequentially added in the step (1) to obtain the mixture A.
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