CN114045210A

CN114045210A - Microorganism detection counting device and counting method thereof

Info

Publication number: CN114045210A
Application number: CN202111349192.3A
Authority: CN
Inventors: 路晓锋; 元小强; 杨颖�; 赵敏; 丁宁
Original assignee: Guangdong Yuegang Water Supply Co ltd; Chinese University of Hong Kong Shenzhen
Current assignee: Guangdong Yuegang Water Supply Co ltd; Chinese University of Hong Kong Shenzhen
Priority date: 2021-11-15
Filing date: 2021-11-15
Publication date: 2022-02-15

Abstract

The application provides a microorganism detection counting device and a counting method thereof, belonging to the field of microorganism detection. The counting device comprises a visual counting platform, a lifting platform, a culture chamber and a transfer mechanism; wherein, the culture platform in the culture chamber is used for colony culture; the clamping piece, the first driving piece and the second driving piece in the transfer mechanism are respectively used for realizing the functions of clamping and overturning the culture dish and transferring the culture dish from the culture platform to the bearing mechanism; the elevating system of elevating platform is used for shifting the culture dish on the bearing mechanism to the position that the vision counting platform can carry out the shooting and carry out intelligent counting. The device is used for counting bacterial colonies, so that the purpose of real-time intelligent counting of the bacterial colonies through a filter membrane method can be achieved, and the problems that the counting is not accurate and the result has hysteresis in the existing filter membrane method are solved.

Description

Microorganism detection counting device and counting method thereof

Technical Field

The application relates to the field of microorganism detection, in particular to a microorganism detection counting device and a counting method thereof.

Background

The filter membrane method is a commonly used evaluation method for the capability assessment of laboratory microorganisms in China, and is widely applied to the field of water quality detection. In a long period of time, the detection process of the technology is operated manually, and the technology has great subjectivity; meanwhile, there is a certain hysteresis in the results by the way of counting after a long culture.

At present, aiming at the problem, intelligent counting equipment capable of counting aiming at a filter membrane method is prepared at home and abroad, bacterial colony counting can be carried out only after culture time is finished, and real-time intelligent counting cannot be realized.

Disclosure of Invention

The application aims to provide a microorganism detection counting device and a counting method thereof so as to achieve the purpose of realizing real-time intelligent counting of bacterial colonies through a filter membrane method.

The embodiment of the application is realized as follows:

in a first aspect, the embodiments of the present application provide a microorganism detection and counting apparatus, including: visual counting platform, elevating platform, cultivation cavity and transfer mechanism.

The elevating platform includes elevating system and bears the weight of the mechanism, and elevating system is used for the drive to bear the weight of the mechanism and goes up and down to make and bear the weight of the mechanism and can be between primary importance and second place reciprocating motion, primary importance is the position that vision count platform can carry out the shooting, and the second place is located one side that vision count platform was kept away from to primary importance.

The culture chamber has a culture platform for placing a culture dish.

The transfer mechanism is provided with a clamping piece, a first driving piece and a second driving piece, the clamping piece is used for clamping the culture dish, the first driving piece is used for overturning the bottom of the clamping piece to the top facing the counting device, and the second driving piece is used for transferring the culture dish from the culture platform to the bearing mechanism at the second position.

In the technical scheme, the culture platform in the culture chamber is used for bacterial colony culture, so that the normal growth of bacterial colonies for counting in a culture dish is ensured; the clamping piece, the first driving piece and the second driving piece in the transfer mechanism are respectively used for realizing the functions of clamping and overturning the culture dish and transferring the culture dish from the culture platform to the bearing mechanism; the elevating system of elevating platform is used for shifting the culture dish on the bearing mechanism to the position that the vision counting platform can carry out the shooting and carry out intelligent counting to the realization carries out real-time intelligent counting's purpose to the bacterial colony through the filter membrane method, thereby compromises and solves the inaccurate and result of current filter membrane method count and has the problem of hysteresis quality.

In some alternative embodiments, the clamping piece is provided with clamping parts which are distributed along the height direction of the counting device at intervals and are used for clamping the upper surface and the lower surface of the culture dish; first recess has all been seted up towards transfer mechanism's direction to culture platform and bearing mechanism, and first recess is used for holding the holder.

In the technical scheme, the upper surface and the lower surface of the culture dish are clamped by the clamping piece, so that the culture dish is better in stability when being clamped, and the culture dish is prevented from falling off from the clamping piece or the body and the cover body of the culture dish are prevented from being separated; the setting of first recess, the holder of being convenient for presss from both sides the culture dish and gets, is favorable to improving counting efficiency.

In some optional embodiments, a clamping cavity is arranged between the clamping parts which are distributed at intervals, and an anti-skid buffer structure is arranged on one side of each clamping part close to the corresponding cavity.

Among the above-mentioned technical scheme, the stability when antiskid buffer structure's setting is carried out the centre gripping in order to further strengthen the holder to the culture dish, prevents the culture dish better and drops.

In some optional embodiments, a second groove for embedding the culture dish is formed at the top of the bearing mechanism.

Among the above-mentioned technical scheme, the setting of second recess is arranged in holding the culture dish, prevents that the culture dish from droing from bearing the mechanism at the in-process that goes up and down, simultaneously, is convenient for fix a position the position that makes things convenient for vision count platform at bearing the mechanism top and shoot with the culture dish.

In some alternative embodiments, the visual counting platform comprises a stop; the limiting piece is used for abutting against the bearing mechanism when the bearing mechanism moves from the second position to the first position so as to prevent the bearing mechanism from continuously moving.

In the technical scheme, the limiting part is arranged to block the bearing mechanism at the position where the visual counting platform can shoot, the positioning mode is convenient and accurate, the shooting effect is ensured, and the counting accuracy is increased; meanwhile, the subsequent step of focal length adjustment is simplified, and the counting time is saved, so that the counting efficiency is improved.

In some alternative embodiments, the limiter is ring-shaped, with a middle cavity in the middle of the limiter; when the bearing mechanism is positioned at the first position, the culture dish borne by the bearing mechanism is positioned in the hollow cavity of the limiting piece; the light sources of the visual counting platform are annularly distributed on the upper surface of the limiting part.

In the technical scheme, the limiting piece is annular, so that a better stabilizing effect can be provided for a culture dish in the bearing mechanism, and the annular structure is convenient to process; the light source annular distribution of vision count platform is in the upper surface of locating part for the light source shines evenly and coverage is wide, thereby ensures the effect of shooting.

In some optional embodiments, an annular protrusion is protruded from the top of the bearing mechanism, and when the bearing mechanism is located at the first position, an outer wall of the annular protrusion abuts against an inner wall of the stopper.

Among the above-mentioned technical scheme, the bellied setting of annular can make bearing mechanism and locating part butt together when the primary importance for the position location of shooing is more accurate, overall structure is more stable.

In some optional embodiments, the visual counting platform further comprises a fine adjustment slider, and the fine adjustment slider is used for adjusting the position of the camera of the visual counting platform in a preset plane, and the preset plane is perpendicular to the height direction of the counting device.

Among the above-mentioned technical scheme, the setting of fine setting slider can further improve the accuracy of shooing the position, the effect of guarantee shooting.

In some optional embodiments, the transfer mechanism further comprises a third driving member for driving the clamping member to move up and down along the height direction of the counting device, and the culture chamber is provided with a plurality of culture platforms along the height direction of the counting device;

optionally, the culture chamber is provided with a rotating shaft along the height direction of the counting device, and the culture chamber can rotate around the rotating shaft; the culture chamber is provided with a plurality of rows of culture platforms, each row of culture platforms are distributed at intervals along the height direction of the counting device, and the plurality of rows of culture platforms are distributed at intervals along the circumferential direction of the culture chamber.

According to the technical scheme, the number of the culture platforms in the culture chamber can be greatly increased by arranging the rotating shaft and the multiple rows of culture platforms in the culture chamber, namely the number of the culture dishes is greatly increased, so that the real-time counting of more bacterial colonies in the culture dishes is realized; meanwhile, the third driving piece is arranged, so that the clamping piece can reach the culture platform at a specific height position in the culture chamber, and the culture dish can be clamped.

In a second aspect, the embodiments of the present application provide a method for detecting and counting microorganisms, which is performed by using the counting device of the first aspect, and includes:

the culture dish is clamped by the clamping piece; the first driving piece turns the bottom of the clamping piece to the top facing the counting device so as to turn the culture dish up and down; the second driving piece transfers the culture dish from the culture platform to the bearing mechanism at a second position; the culture dish is transferred from the second position to the first position by the lifting mechanism; the visual counting platform counts the colonies in the petri dish.

In the technical scheme, the clamping piece, the first driving piece and the second driving piece are respectively used for realizing the functions of clamping and overturning the culture dish and transferring the culture dish from the culture platform to the bearing mechanism; the elevating system of elevating platform is used for shifting the culture dish on the bearing mechanism to the position that vision counting platform can carry out the shooting and carry out intelligent counting to the realization carries out real-time intelligent counting's purpose to the bacterial colony through the filter membrane method.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.

FIG. 1 is a first view of a microorganism detection and counting device provided in an embodiment of the present application;

FIG. 2 is a first view of a transferring mechanism of a microorganism detection and counting device provided in an embodiment of the present application;

FIG. 3 is a first view of a visual counting platform of a microorganism detection and counting device provided in an embodiment of the present application;

FIG. 4 is a first view of the elevating platform of a microorganism detecting and counting device provided in the embodiments of the present application;

FIG. 5 is a first view of a culture chamber of a microorganism detection and counting device provided in the embodiment of the present application;

FIG. 6 is a cross-sectional view of a culture chamber of a microorganism detection and counting device provided in an embodiment of the present application.

Icon: 100-a microorganism detection and counting device; 110-a transfer mechanism; 111-a clamp; 1111-a clamping part; 112-a first drive member; 113-a second drive member; 114-a third drive member; 120-a visual counting platform; 121-fine tuning of the slider; 122-a camera; 123-a light source; 124-a limiting member; 130-a lifting platform; 131-a carrying mechanism; 1311 — first groove; 1312-a second groove; 132-a lifting mechanism; 140-a culture chamber; 141-culture platform; 142-a rotating shaft;

a-height direction of the counting device.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present application, it is to be noted that the terms "upper", "lower", "vertical", "horizontal", "inner", "outer", and the like refer to orientations or positional relationships based on orientations or positional relationships shown in the drawings or orientations or positional relationships conventionally found in use of products of the application, and are used for convenience in describing the present application and for simplicity in description, and do not indicate or imply that the referred devices or elements must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present application. Furthermore, the terms "first," "second," and the like are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal", "vertical" and the like do not imply that the components are required to be absolutely horizontal or vertical, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present application, it is further noted that, unless expressly stated or limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.

The inventor researches and discovers that certain subjectivity and hysteresis exist when the intelligent counting of the bacterial colonies is carried out through a filter membrane method for a long time, and the counting result is inaccurate. Although intelligent equipment capable of counting by aiming at a filter membrane method is prepared at home and abroad, the problem of hysteresis is not well solved. The reason for this is that when colony counting is performed by a filter membrane method, it is necessary that the culture dish faces upward, and in the colony culture process by the filter membrane method, it is necessary that the culture dish faces upward. At present, because the upset of culture dish can't be realized to intelligent counting equipment at home and abroad, also can't carry out the real-time intelligent count of filtering the embrane method through intelligent equipment yet.

The inventor researches and discovers that on the basis of the existing intelligent counting equipment for bacterial colonies, the purpose of intelligently counting the bacterial colonies in real time through a filter membrane method can be achieved by further endowing the intelligent counting equipment with a function of turning a culture dish, so that the problems that the counting is not accurate through the existing filter membrane method and the results have hysteresis are solved.

Referring to fig. 1, in a first aspect, the present disclosure provides a microorganism detection and counting apparatus 100, which includes a visual counting platform 120, a lifting platform 130, a culture chamber 140, and a transfer mechanism 110.

In the drawings of the present application, the first view is a front view in the set position with reference to fig. 1.

The lifting platform 130 includes a lifting mechanism 132 and a bearing mechanism 131, the lifting mechanism 132 is configured to drive the bearing mechanism 131 to lift, so that the bearing mechanism 131 can reciprocate between a first position and a second position, the first position is a position where the visual counting platform 120 can perform shooting, and the second position is located on a side of the first position away from the visual counting platform 120.

The culture chamber 140 has a culture platform 141 for placing a culture dish.

The transferring mechanism 110 has a holding member 111, a first driving member 112 and a second driving member 113, the holding member 111 is used for holding the culture dish, the first driving member 112 is used for turning the bottom of the holding member 111 to the top facing the microorganism detecting and counting device 100, and the second driving member 113 is used for transferring the culture dish from the culture platform 141 to the carrying mechanism 131 at the second position.

In the present application, the culture platform 141 in the culture chamber 140 is used for colony culture, so as to ensure normal growth of colonies for counting in the culture dish; the clamping member 111, the first driving member 112 and the second driving member 113 in the transferring mechanism 110 are respectively used for clamping and overturning the culture dish and transferring the culture dish from the culture platform 141 to the carrying mechanism 131; the lifting mechanism 132 of the lifting platform 130 is used for transferring the culture dish on the bearing mechanism 131 to the position where the visual counting platform 120 can shoot for intelligent counting, so that the purpose of real-time intelligent counting of bacterial colonies through a filter membrane method is achieved, and the problems that the counting is not accurate and the result has hysteresis in the existing filter membrane method are solved.

It is understood that, as an example, the microorganism detection and counting device 100 may further be provided with a bottom plate for mounting and fixing various structures in the microorganism detection and counting device 100. The visual counting platform 120 includes a light source 123, a camera 122, a base, a first lifting slider, a second lifting slider, and a column. The base is used for the installation of each structure in the visual technology platform, fixed, and first lifting slide and second lifting slide all are connected with the stand through dovetail and gear, and can follow the stand up-and-down motion for carry out the focus and adjust, and camera 122 and light source 123 are fixed respectively at first lifting slide and second lifting slide.

For ease of understanding, the working principle of the visual counting platform 120 is described here: when carrying out the bacterial colony count, move along the stand up and down through first lift slider and second lift slider earlier and carry out focus adjustment, then utilize camera 122 to carry out the collection of picture information to the target area, simultaneously, guarantee the shooting quality of picture through adjusting light source 123. In addition, the visual counting platform 120 is connected to a computer, the photographed picture information is stored in the computer, and the picture information of the corresponding bacterial colony is processed by the computer system, so that the intelligent counting of the bacterial colony can be realized.

As an example, the lifting mechanism 132 includes a motor and a linear module, and the linear module can drive the carrying device to lift and lower through the motor drive. In addition, the lifting platform 130 is provided with a supporting seat, the bearing device is fixed on the linear module by screws, and the linear module is fixed on the supporting seat by screws.

In addition, the vision counting platform 120 and the elevating platform 130 may be provided in an integrated form.

As an example, the visual counting platform 120 and the lifting platform 130 are connected to the same column, specifically: the first lifting slide block and the second lifting slide block in the visual counting platform 120 and the linear module in the lifting platform 130 are fixed on the same column to slide up and down.

Further, in consideration of the possibility that the camera 122 and the culture dish may have a position error in a plane perpendicular to the height direction a of the counting device when the vision counting platform 120 performs the positioning shooting, in order to ensure the shooting accuracy, a structure capable of adjusting the position of the camera 122 in the plane may be added to the vision counting platform 120.

As an example, the vision counting platform 120 is provided with a fine adjustment slider 121, the camera 122 is fixed to the fine adjustment slider 121 by a clamping tool, and the fine adjustment slider 121 is fixed to the first elevation slider by a screw. It should be noted that the plane perpendicular to the height direction a of the counting device includes the front and rear directions and the left and right directions, and the front and rear directions are referred to herein.

Referring to fig. 2, it should be noted that, in consideration of the fact that the culture dish may loosen or fall off or the body and the cover of the culture dish may be separated during the clamping of the culture dish by the clamping member 111, in order to make the culture dish have better stability during the clamping, the clamping manner may be improved.

As an example, the clamping member 111 has clamping portions 1111 that are relatively spaced apart in the height direction a of the counting device, and the upper and lower surfaces of the culture dish are clamped by the clamping portions 1111; in other embodiments, the clamping member 111 may be provided with clamping portions 1111 spaced apart from each other in a direction perpendicular to the height direction a of the counting device, and in this case, the side surface of the culture dish may be clamped by the clamping portions 1111.

Meanwhile, in consideration of convenience in the operation of clamping the culture dish by the clamping member 111, the structures of the bearing mechanism 131 and the culture platform 141 can be improved.

As an example, the culture platform 141 and the carrying mechanism 131 are both opened with a first groove 1311 for accommodating the clamping member in the direction toward the transferring mechanism 110. In other embodiments, the first groove 1311 may be provided in a form penetrating the culture platform 141 and the carrying mechanism 131 in the height direction a of the counting device.

In addition, in order to further enhance the stability of the holder 111 when holding the culture dish and prevent the culture dish from falling off, the holder 1111 of the holder 111 may be modified.

As an example, one side of the clamping portion 1111 near the cavity is provided with an anti-slip buffer structure, which may be configured as an anti-slip rubber layer.

Referring to fig. 3, further, in order to prevent the culture dish from falling off from the bearing mechanism 131 in the process of ascending and descending, and meanwhile, the visual counting platform 120 is convenient to position, so that the counting efficiency is improved, the structure of the bearing mechanism 131 can be further improved.

As an example, the top of the carrying mechanism 131 is opened with a second groove 1312 for embedding a culture dish, and the second groove 1312 is circular. In other embodiments, the shape of the second recess 1312 may be rectangular, triangular, elliptical, and regular polygonal, such as square, regular pentagon, regular hexagon, etc., as long as the second recess 1312 can accommodate a culture dish.

It should be noted that, in order to ensure that the culture dish carried on the carrying mechanism 131 can accurately reach the position where the vision counting platform 120 can shoot, the shooting effect is ensured, and thus the counting accuracy is increased; meanwhile, the subsequent steps of focal length adjustment are simplified, the counting time is saved, and the structure of the existing visual counting platform 120 can be improved.

As an example, the vision counting platform 120 is provided with a limiting member 124, and the limiting member 124 is fixed to the second lifting slider by a screw. The limiting member 124 is configured to abut against the bearing mechanism 131 when the bearing mechanism 131 moves from the second position to the first position, so as to prevent the bearing mechanism 131 from moving further.

At the same time, it is considered that better stability and ease of component process preparation can be provided for the culture dish in the carrying mechanism 131.

As an example, the stopper 124 is provided in a ring shape; in other embodiments, the limiting member 124 may also be configured as a rectangle, a triangle, an ellipse, and a regular polygon, such as a square, a regular pentagon, a regular hexagon, etc., it is easy to understand that as long as the limiting member 124 can accommodate the bearing mechanism 131 for positioning and counting.

Referring to fig. 4, in addition, in view of further improving the stability of the overall structure of the bearing mechanism 131 and the limiting member 124, the structure of the bearing mechanism 131 may be further optimized.

As an example, an annular protrusion is protruded from the top of the bearing mechanism 131, and when the bearing mechanism 131 is located at the first position, an outer wall of the annular protrusion abuts against an inner wall of the stopper 124. In other embodiments, the shape of the top convex outer wall of the bearing mechanism 131 may also be set as a rectangle, a triangle, an ellipse, and a regular polygon, such as a square, a regular pentagon, a regular hexagon, etc., it is easy to understand that as long as the bearing mechanism 131 can be accommodated in the limiting member 124 for positioning and counting.

On this basis, in order to make the light source 123 irradiate uniformly and cover a wide range and thus ensure the shooting effect, the position where the light source 123 is disposed may be considered in consideration of the need of brightness adjustment during shooting.

As an example, the light sources 123 are annularly distributed on the upper surface of the limiting member 124 and fixed on the limiting member 124 by screws; in other embodiments, the light sources 123 may also be distributed on the lower surface, the inner surface and the side surface of the limiting member 124. In other embodiments, the light source 123 and the limiting member 124 may be provided in a separate body.

It will be appreciated that the connection between the gripper 111, the first drive member 112 and the second drive member 113 in the transfer mechanism 110 is adjustable.

As an example, the clamping member 111 is connected to the first driving member 112 and then connected to the second driving member 113. In other embodiments, the clamping member 111 is connected to the second driving member 113 first, and then connected to the first driving member 112.

In addition, in order to provide the transfer mechanism 110 with mobility in the height direction a of the counting device, the structure of the transfer mechanism 110 may be improved. As an example, the transfer mechanism 110 is provided with a third drive 114. Accordingly, the connection relationship among the clamping member 111, the first driving member 112, the second driving member 113 and the third driving member 114 is adjustable.

As an example, the clamping member 111 is connected to the first driving member 112, the second driving member 113, and the third driving member 114. In other embodiments, the clamping member 111 is connected to the second driving member 113, the first driving member 112, and the third driving member 114.

In addition, in order to meet the requirement of grabbing culture dishes with different radius ranges, driving members capable of increasing the grabbing radius can be further arranged in the transfer mechanism 110, the driving members are connected between the clamping member and the third driving member 114 along the height direction a perpendicular to the counting device, the connection sequence of the driving members and the first driving member 112 and the second driving member 113 is not limited, and the number of the driving members can be adjusted according to the grabbing radius required actually.

As an example, the transfer mechanism 110 is further provided with a fourth driving member for increasing the gripping radius, and the fourth driving member is connected between the first driving member 112 and the second driving member 113.

Referring to fig. 5 to 6, further, in order to increase the number of the culture platforms 141 in the culture chamber 140, i.e. to increase the number of the culture dishes greatly, the real-time counting of more colonies in the culture dishes is realized; meanwhile, the transfer mechanism 110 and the culture chamber 140 can be matched easily, the culture dish at any position in the culture chamber 140 can be clamped, and the structure of the culture chamber 140 can be improved.

As an example, the culture chamber 140 is provided with a plurality of culture platforms 141 in the height direction a of the counting device; the culture chamber 140 is provided with a rotating shaft 142 along the height direction a of the counting device, and the culture chamber 140 can rotate around the rotating shaft 142; the culture chamber 140 is provided with a plurality of rows of culture platforms 141, each row of culture platforms 141 is distributed at intervals along the height direction a of the counting device, and the plurality of rows of culture platforms 141 are distributed at intervals along the circumferential direction of the culture chamber 140.

It should be noted that the form of the rotating shaft 142 is not limited, and it can be a short shaft form of the rotating platform, and can also be a long shaft form throughout the height of the microorganism detecting and counting device 100, and it can be understood that it is only necessary to rotate the culture platform 141 in the culture chamber 140 around the rotating shaft 142. As an example, the culture chamber 140 further includes a rotation stage, a turntable mount, a support column, an arc-shaped support plate, a cylinder, a bearing, a top flange, and a bottom flange. The revolving stage and the cooperation of revolving stage mount pad performance axis of rotation 142's function, the revolving stage passes through the fix with screw on the revolving stage mount pad, and the rotation axis passes through the fix with screw on the revolving stage mount pad, and the bottom flange passes through the fix with screw on the rotation axis, and the barrel passes through screw and bottom flange joint, and the top flange passes through the fix with screw on the barrel, and the backup pad passes through bearing, screw and top flange joint, and the arc backup pad passes through screw and mount pad, backup pad connection.

In a second aspect, the present application provides a method for detecting and counting microorganisms, which is performed by using the microorganism detection and counting apparatus 100 of the first aspect, and includes:

the clamping piece 111 clamps the culture dish; the first driving member 112 turns the bottom of the clamping member 111 to the top of the microorganism detection and counting device 100, so that the culture dish is turned upside down; the second driving member 113 transfers the culture dish from the culture platform 141 to the carrying mechanism 131 at the second position; the lift mechanism 132 transfers the culture dish from the second position to the first position; the visual counting platform 120 counts the colonies in the culture dish.

In the present application, the clamping member 111, the first driving member 112 and the second driving member 113 are respectively used for clamping and turning the culture dish and transferring the culture dish from the culture platform 141 to the carrying mechanism 131; the lifting mechanism 132 of the lifting platform 130 is used for transferring the culture dish on the bearing mechanism 131 to the position where the visual counting platform 120 can shoot so as to carry out intelligent counting, so that the purpose of carrying out real-time intelligent counting on the bacterial colony through a filter membrane method is achieved.

It should be noted that the counting method may also be modified for continuous real-time intelligent counting of normally growing colonies in the culture chamber 140.

As an example, the holder 111 holds a culture dish; the first driving member 112 turns the bottom of the clamping member 111 to the top of the microorganism detection and counting device 100, so that the culture dish is turned upside down; the second driving member 113 transfers the culture dish from the culture platform 141 to the carrying mechanism 131 at the second position; the lift mechanism 132 transfers the culture dish from the second position to the first position; the visual counting platform 120 counts the colonies in the culture dish.

After the counting is finished, the culture dish is clamped by the clamping piece 111; the first driving member 112 turns the top of the clamping member 111 to face the bottom of the microorganism detection and counting device 100, so that the culture dish is turned upside down; the second drive member 113 transfers the culture dish from the carrier 131 in the second position to the culture platform 141.

To sum up, this application embodiment can carry out the function of upset to the culture dish through giving intelligent counting equipment to reach and realize the purpose of the real-time intelligent count of bacterial colony through the filter membrane method, thereby compromise and solve the inaccurate and result of current filter membrane method count and have the problem of hysteresis quality.

The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A microorganism detection counting device, comprising:

a visual counting platform;

the lifting platform comprises a lifting mechanism and a bearing mechanism, the lifting mechanism is used for driving the bearing mechanism to lift so as to enable the bearing mechanism to reciprocate between a first position and a second position, the first position is a position where the visual counting platform can shoot, and the second position is located on one side, away from the visual counting platform, of the first position;

a culture chamber having a culture platform for placing a culture dish; and

the transfer mechanism is provided with a clamping piece, a first driving piece and a second driving piece, the clamping piece is used for clamping the culture dish, the first driving piece is used for overturning the bottom of the clamping piece to the top of the counting device, and the second driving piece is used for transferring the culture dish from the culture platform to the bearing mechanism at the second position.

2. The counting device of claim 1, wherein the clamping member has clamping portions spaced relatively in a height direction of the counting device for clamping upper and lower surfaces of the culture dish; first grooves are formed in the culture platform and the bearing mechanism in the direction towards the transfer mechanism, and the first grooves are used for accommodating the clamping pieces.

3. The counting device of claim 2, wherein a clamping cavity is formed between the clamping portions which are distributed at intervals, and an anti-slip buffer structure is arranged on one side of each clamping portion close to the corresponding cavity.

4. The counting device according to any one of claims 1 to 3, wherein a second groove for embedding the culture dish is formed at the top of the bearing mechanism.

5. The counting device of any one of claims 1 to 3, wherein the visual counting platform comprises a stop; the limiting part is used for abutting against the bearing mechanism when the bearing mechanism moves from the second position to the first position so as to prevent the bearing mechanism from continuously moving.

6. The counting device of claim 5, wherein the retainer is ring-shaped, and the retainer has a middle cavity in the middle; when the bearing mechanism is located at the first position, the culture dish borne by the bearing mechanism is located in the hollow cavity of the limiting piece; and light sources of the visual counting platform are annularly distributed on the upper surface of the limiting part.

7. The counting device of claim 6, wherein an annular protrusion is protruded from a top of the supporting mechanism, and when the supporting mechanism is located at the first position, an outer wall of the annular protrusion abuts against an inner wall of the limiting member.

8. The counting device according to any one of claims 1 to 3, wherein the visual counting platform further comprises a fine adjustment slider for adjusting the position of the camera of the visual counting platform in a preset plane, and the preset plane is perpendicular to the height direction of the counting device.

9. The counting device according to any one of claims 1 to 3, wherein the transfer mechanism further comprises a third driving member for driving the holding member to move up and down along the height direction of the counting device, and the culture chamber is provided with a plurality of culture platforms along the height direction of the counting device;

10. A method for detecting and counting microorganisms, which is carried out by using the counting device according to any one of claims 1 to 9, and comprises the steps of:

the culture dish is clamped by the clamping piece; the first driving piece turns the bottom of the clamping piece to face the top of the counting device so as to turn the culture dish up and down; the second drive transfers the culture dish from the culture platform to the carrier mechanism at the second position; the lifting mechanism transferring the culture dish from the second position to the first position; the visual counting platform counts the colonies in the culture dish.