CN114034225A - Method for testing movement precision of injection needle under microscope - Google Patents
Method for testing movement precision of injection needle under microscope Download PDFInfo
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- CN114034225A CN114034225A CN202111427586.6A CN202111427586A CN114034225A CN 114034225 A CN114034225 A CN 114034225A CN 202111427586 A CN202111427586 A CN 202111427586A CN 114034225 A CN114034225 A CN 114034225A
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- injection needle
- equally divided
- microscope
- measuring tool
- scale
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- 238000002347 injection Methods 0.000 title claims abstract description 59
- 239000007924 injection Substances 0.000 title claims abstract description 59
- 238000012360 testing method Methods 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 22
- 230000000007 visual effect Effects 0.000 claims abstract description 16
- 238000011166 aliquoting Methods 0.000 claims description 6
- 238000005259 measurement Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000001531 micro-dissection Methods 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B5/00—Measuring arrangements characterised by the use of mechanical techniques
Abstract
The invention relates to the technical field of micromanipulation, and discloses a method for testing the movement precision of a shooting needle under a microscope, which comprises the following steps: step 1: placing the measuring tool in the visual field range of the microscope, acquiring a scale image, and removing the measuring tool; step 2: equally dividing the scale image along the extension direction of the measuring tool by using tool software to form a plurality of equally divided scales, forming equally divided units between adjacent equally divided scales to obtain equally divided scale images, wherein the width of each equally divided unit is smaller than the minimum unit length of the measuring tool; and step 3: and moving the injection needle to the visual field range of the microscope containing the equally-divided scale images to test the moving precision of the injection needle. The invention relates to a method for testing the movement precision of a microscope injection needle, which improves the movement precision of the injection needle under the condition of low cost.
Description
Technical Field
The invention relates to the technical field of micromanipulation, in particular to a method for testing the movement precision of a shooting needle under a microscope.
Background
At present, the method is often applied to micromanipulation technology in the technical fields of embryo transplantation, microdissection, microinjection and the like, but the size of an operation object is generally in the micron level, and the existing mode is a laser interferometer, but the laser interferometer is expensive; in the other prior art, the minimum precision of a measuring tool (such as a micrometer) is 10 micrometers, a technician in a general situation finds that the measuring tool cannot meet the requirement of the measuring precision of 1 micrometer or a smaller numerical value in manual operation, and mechanical equipment cannot avoid certain self or installation errors, so that the micrometer and an injection needle are not in the same focal plane under a microscope, the micrometer and the injection needle cannot be adjusted to be in a clear state, and the moving precision of the injection needle is difficult to test.
Disclosure of Invention
The purpose of the invention is: provided is a movement accuracy test method for realizing high-accuracy movement of an injection needle at low cost.
In order to achieve the above object, the present invention provides a method for testing the movement accuracy of a needle under a microscope, comprising the steps of:
step 1: placing a measuring tool in the visual field range of a microscope, acquiring a scale image, and removing the measuring tool;
step 2: equally dividing the scale image along the extension direction of the measuring tool by using tool software to form a plurality of equally divided scales, forming equally divided units between adjacent equally divided scales to obtain an equally divided scale image, wherein the width of each equally divided unit is smaller than the minimum unit length of the measuring tool;
and step 3: and setting the equally-divided scale image in the visual field range of the microscope, and moving the injection needle in the visual field range of the microscope to acquire the movement precision of the injection needle.
Preferably, if the aliquot unit obtained in the step 2 does not satisfy the requirement of the movement accuracy test of the injection needle, the step 1 to the step 3 are repeatedly operated or the step 2 to the step 3 are repeatedly operated.
Preferably, during the repetition, the measuring tool is changed in step 1 and/or the width of the aliquoting unit is adjusted in step 2 to adjust the movement accuracy of the injection needle.
Preferably, in step 1, the operation position of the injection needle is within the visual field range, the injection needle is moved by a set number of the graduation marks, the width of the graduation unit is reduced when the injection needle exceeds the operation position, and the width of the graduation unit is increased when the injection needle does not reach the operation position.
Preferably, in step 1, before the measurement tool is placed, a field of view of the microscope is acquired as an initial image, the initial image is equally divided to obtain an initial equally divided image, the measurement tool is placed in the field of view of the microscope, a scale initial image is acquired, and the initial equally divided image and the scale initial image are correspondingly processed to obtain the scale image.
Preferably, in step 2, at least one of the graduation marks is aligned with the graduation mark of the measuring tool in the graduation image in a direction perpendicular to the extending direction of the measuring tool.
Preferably, the measuring means is a micrometer, the minimum scale of the micrometer is 10 micrometers, and in the step 2, the minimum unit length of the micrometer is divided into 10 equal parts to obtain the divided units with the width of 1 micrometer.
Preferably, the number of the scale images is one.
Preferably, the extending direction of the measuring tool in the step 1 is an X-axis direction or a Y-axis direction.
Compared with the prior art, the method for testing the movement precision of the injection needle under the microscope has the beneficial effects that: in step 1, a scale image of the measuring tool including a scale of a unit length is acquired, and the scale of the unit length in the scale image is used as a comparison basis for equally dividing the width of the divided cell formed in step 2. In step 2, the scale image is equally divided, the width of the equally dividing unit is smaller than the minimum unit length of the measuring tool, so that the equally dividing scale is a unit length value smaller than the minimum unit length value of the measuring tool, and the movement test precision of the injection needle is improved. The moving needle is moved to the visual field range of the microscope containing the equally-divided scale images, so that high-precision moving operation test can be realized, and high-precision moving of the injection needle is improved under the condition of low cost.
Drawings
FIG. 1 is a schematic illustration of an equally scaled image according to an embodiment of the present invention.
Detailed Description
The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
In the description of the present invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. used herein are used to indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
In the description of the present invention, it should be understood that the terms "connected," "fixed," and the like are used in a broad sense, and for example, the terms "connected," "connected," and "fixed" may be fixed, detachable, or integrated; the connection can be mechanical connection or welding connection; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
As shown in fig. 1, a method for testing the movement accuracy of a microscope injection needle according to a preferred embodiment of the present invention includes the following steps:
step 1: placing the measuring tool in the visual field range of the microscope, acquiring a scale image, and removing the measuring tool;
step 2: equally dividing the scale image along the extension direction of the measuring tool by using tool software to form a plurality of equally divided scales, forming equally divided units between adjacent equally divided scales to obtain equally divided scale images, wherein the width of each equally divided unit is smaller than the minimum unit length of the measuring tool;
and step 3: and setting the equally-divided scale image in the visual field range of the microscope, and moving the injection needle in the visual field range of the microscope to acquire the movement precision of the injection needle.
In the method for testing the movement accuracy of the injection needle under the microscope, a scale image of a measuring tool containing unit length scales is acquired in step 1, and the unit length scales in the scale image are used as a comparison basis for the width of an equally divided unit formed by equally dividing in step 2. In step 2, the scale image is equally divided, the width of the equally dividing unit is smaller than the minimum unit length of the measuring tool, and the equally dividing scale is further made to be a unit length value smaller than the minimum unit length value of the measuring tool, so that the movement test precision of the injection needle is improved. The moving needle is moved to the visual field range of the microscope containing the equally divided scale images, so that the high-precision moving operation test can be realized, and the high-precision movement of the injection needle is improved under the condition of low cost.
Further, if the aliquot unit obtained in the step 2 does not meet the requirement of the movement precision test of the injection needle, repeating the steps 1 to 3 or repeating the steps 2 to 3.
When the injection needle performs injection operation on an injection target, the injection needle needs to inject needle liquid in the injection needle to an operation position of the injection target to complete the injection operation, the injection needle moves according to an equal division scale value in an equal division scale image, if the injection needle can inject the needle liquid to the operation position, an operation task is completed, and the equal division scale image can meet the requirement of the movement precision of the injection needle. If the needle is not injected to the operating position due to a certain distance between the needle and the operating position when the needle is injected, the equally divided scale image cannot meet the requirement of the movement precision of the needle. And (3) repeating the step (1) and the step (2) to re-form the equally divided scale image, or repeating the step (2) to re-equally divide the scale image, and then verifying whether the equally divided scale image meets the movement precision requirement of the injection needle or not through the step (3).
Further, during the repetition, the measuring tool is changed in step 1 and/or the width of the aliquoting unit is adjusted in step 2 to adjust the moving accuracy of the injection needle. The reasons for the deviation of the width of the aliquoting unit include the inaccuracy of the scale of the measuring tool and/or the deviation of the amount of the aliquoting, and the precision of the movement of the injection needle is adjusted by replacing the measuring tool and/or adjusting the width of the aliquoting unit.
Furthermore, in step 1, the operating position of the injection needle is in the visual field, the injection needle moves a set number of equal divisions, the operating position refers to the injection position of the injection needle in the injection target, the number of equal divisions corresponding to the movement of the injection needle is estimated according to different experimental requirements, if the injection needle exceeds the operating position, the width of the single equal division unit is reduced, and if the injection needle does not reach the operating position, the width of the single equal division unit is reduced, and if the width of the single equal division unit is too small, the width of the equal division unit is increased.
Further, in step 1, before the measurement tool is placed, a field of view of the microscope is acquired as an initial image, the initial image is equally divided to obtain an initial equally divided image, the measurement tool is placed in the field of view of the microscope to obtain a scale initial image, and the initial equally divided image and the scale initial image are correspondingly processed to obtain a scale image. The initial equally dividing image and the initial scale image are processed correspondingly, and the moving precision of the injection needle is improved as the basis of the equally dividing calibration in the step 2. The correspondence processing means that the dividing direction of the initial divided image is kept the same as the extending direction of the scale, and one side of at least one division in the initial divided image is aligned with the scale on the scale.
Further, in step 2, at least one of the graduation marks is aligned with the graduation mark of the measuring tool in the graduation image in a direction perpendicular to the extending direction of the measuring tool. Through the corresponding arrangement of the equal division scale and the scale of the measuring tool, the equal division quantity or the equal division unit width of the scale image is convenient to set, and the equal division setting accuracy of the equal division scale image is improved.
More specifically, the measuring tool is a micrometer, the minimum scale of the micrometer is 10 micrometers, and in step 2, the minimum unit length of the micrometer is equally divided into 10 parts to obtain an equally divided unit with the width of 1 micrometer, so that the requirement of the measuring precision of 1 micrometer is met.
Furthermore, the number of the scale images is one, a plurality of equally divided scale rows are formed by equally dividing only one scale image along the extending direction of the measuring tool in the step 2, the number of equally divided image images is small, the operation method is simple, convenient and fast, and the operation difficulty of the injection needle movement precision testing method is reduced. More specifically, a scale image is formed by combining one initial equally divided image and one scale initial image in a one-to-one correspondence manner.
In the scale image, a measuring tool is adjusted or placed according to actual requirements, and further, in the step 1, the extending direction of the measuring tool is an X-axis direction or a Y-axis direction. The measuring tool extends in the X-axis direction or the Y-axis direction for operation in conjunction with a coordinate system.
In summary, the embodiment of the present invention provides a method for testing movement accuracy of a needle under a microscope, in which tool software is used to equally divide a scale image along an extending direction of a measuring tool to form a plurality of equally divided scales, and an equally divided unit is formed between adjacent equally divided scales to obtain an equally divided scale image, wherein the width of the equally divided unit is smaller than the minimum unit length of the measuring tool, so as to improve the movement accuracy test of the needle under a low cost.
The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and substitutions can be made without departing from the technical principle of the present invention, and these modifications and substitutions should also be regarded as the protection scope of the present invention.
Claims (9)
1. A method for testing the movement precision of a shooting needle under a microscope is characterized by comprising the following steps: the method comprises the following steps:
step 1: placing a measuring tool in the visual field range of a microscope to obtain a scale image;
step 2: equally dividing the scale image along the extension direction of the measuring tool to form a plurality of equally divided scales, forming equally divided units between adjacent equally divided scales to obtain equally divided scale images, wherein the width of each equally divided unit is smaller than the minimum unit length of the measuring tool;
and step 3: and setting the equally-divided scale image in the visual field range of the microscope, and moving the injection needle in the visual field range of the microscope to acquire the movement precision of the injection needle.
2. The method for testing the movement accuracy of a needle under a microscope according to claim 1, wherein: and if the equal division unit obtained in the step 2 does not meet the requirement of the movement precision test of the injection needle, repeating the steps 1 to 3 or repeating the steps 2 to 3.
3. The method for testing the movement accuracy of a needle under a microscope according to claim 2, wherein: during the repetition, the measuring tool is changed in step 1 and/or the width of the aliquoting unit is adjusted in step 2 to adjust the movement accuracy of the injection needle.
4. The method for testing the movement accuracy of a needle under a microscope according to claim 3, wherein: in the step 1, the operation position of the injection needle is within the visual field, the injection needle moves a set number of the graduation marks, the width of the graduation unit is reduced when the injection needle exceeds the operation position, and the width of the graduation unit is increased when the injection needle does not reach the operation position.
5. The method for testing the movement accuracy of a needle under a microscope according to claim 1, wherein: in the step 1, before the measurement tool is placed, a field of view of the microscope is acquired as an initial image, the initial image is equally divided to obtain an initial equally divided image, the measurement tool is placed in the field of view of the microscope to obtain a scale initial image, and the initial equally divided image and the scale initial image are correspondingly processed to obtain the scale image.
6. The method for testing the movement accuracy of a needle under a microscope according to claim 1, wherein: in step 2, at least one of the graduation marks is aligned with the graduation mark of the measuring tool in the graduation mark image in a direction perpendicular to the extending direction of the measuring tool.
7. The method for testing the movement accuracy of a needle under a microscope according to claim 1, wherein: the measuring tool is a micrometer, the minimum scale of the micrometer is 10 micrometers, and in the step 2, the minimum unit length of the micrometer is equally divided into 10 parts to obtain the equally divided units with the width of 1 micrometer.
8. The method for testing the movement accuracy of a needle under a microscope according to claim 1, wherein: the number of the scale images is one.
9. The method for testing the movement accuracy of a needle under a microscope according to claim 1, wherein: the extending direction of the measuring tool in the step 1 is an X direction or a Y direction.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111427586.6A CN114034225A (en) | 2021-11-25 | 2021-11-25 | Method for testing movement precision of injection needle under microscope |
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| CN202111427586.6A CN114034225A (en) | 2021-11-25 | 2021-11-25 | Method for testing movement precision of injection needle under microscope |
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| CN114034225A true CN114034225A (en) | 2022-02-11 |
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