CN2375316Y - Double-cavity microsyringe with several injection ranges - Google Patents
Double-cavity microsyringe with several injection ranges Download PDFInfo
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- CN2375316Y CN2375316Y CN99228317U CN99228317U CN2375316Y CN 2375316 Y CN2375316 Y CN 2375316Y CN 99228317 U CN99228317 U CN 99228317U CN 99228317 U CN99228317 U CN 99228317U CN 2375316 Y CN2375316 Y CN 2375316Y
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- microinjection
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Abstract
The utility model relates to a double-cavity micro injecting instrument with a plurality of injecting ranges, which is used for micro injecting technology. The utility model uses a transparent tee body to replace a medical injecting device of the existing product, wherein, the tee body is provided with an air discharging piston used for filling hydraulic media and removing the gas in the media and a micro injecting piston driven by a differential screw rod. The two pistons are provided with sealing structures. The tee body and the micro injecting needle are connected by a pipeline. The tee body is arranged on the horizontal part of a vertical angle machine frame and the horizontal part is connected with a machine base. A driving component of the differential screw rod is connected with the vertical part of a vertical angle photo frame. The structure can eliminate the leakage of the hydraulic media and can provide three injecting ranges.
Description
Microinjection instrument (or claiming micro-notes device) is the precision instrument of indispensability when carrying out the microinjection technique application
[1,2]Its effect is with extremely the liquid substance such as the DNA of trace or the intracytoplasmic sperm injection that is in the suspension pump out in the cell of transferring to other in cell in cell or with nucleus.Domestic employed this quasi-instrument major part is the IM series of products from Japanese Cheng Maoshe import at present
[3], structure as shown in Figure 1, its operation principle is to charge white oil 8 in syringe sleeve 4, flexible pipe 3 needle cannula 2, forms hydraulic system, loads onto micropin 1 then, is thrust in the cell by micromanipulator guiding micropin 1; Rotate handwheel 7, drive screw rod 6 precession and pushing syringe piston 5 and in 4, produce positive/negative pressure, positive/negative pressure is aspirated by the tip that is sent to micropin pipe 1 or the injection trace substance by pressure transmission medium white oil 8.The parts 4 and 5 that IM series has a common architectural feature promptly to produce positive/negative pressure all are to adopt injector for medical purpose (capacity 1-3ml) or chromatograph injector (capacity 800 μ l).In theory, the injection volume of microinjection instrument requires its denier, and the injection volume of each cell is 300-500pl (10 when injection DNA
-9Ml)
[4], therefore require microinjection instrument to have the sensitive performance and the accurate performance of height.When being scale Δ φ of handwheel rotation (handwheel is divided into several Δs φ scale, but the Δ ν injection volume of the corresponding reading of each Δ) should there be the material of Δ ν to outpour at the micro needlepoint place, promptly
KΔφ＝Δν (1)
In the formula, K is a gearing factor
K＝S×L (2)
S: piston cross-section is long-pending; L: the pitch of screw rod
N: handwheel graduation number
Microinjector precision η=Δ ν/V herein, V: the injection volume that handwheel one changes
When using, reality finds, when rotating Δ φ, handwheel do not see that needle tip has the material of Δ ν to inject, then turn over several Δs φ time side continuously and see that needle tip has material to outpour, its volume far surpasses needed quantity and then causes the bursting apart of cell, fragmentation, this phenomenon illustrates the insufficient sensitivity of this class injection instrument, causes the accurate performance of injection volume not good.Analyze this class, its main cause of the topology discovery of microinjection instrument is because due to the systematic error of the positive and negative living parts of cutting down output that adopt.Because medical treatment or chromatographic grade syringe sampling device adopt glass and metal material to make, can in syringe sleeve, twitch for making piston, between piston and sleeve pipe certain space must be arranged, be called fit clearance, when dividing the difference grade to get basic deviation Ff according to the IT6 in the Common Difference ﹠ Cooperation standard of GB1800-1804-79, its piston diameter of the syringe of diameter 3mm is actual to be 2.990-2.994mm, and telescopic effective aperture is its difference of 3.006-3.012mm 16-18 μ m.The syringe of diameter 6mm, the piston actual diameter is 5.985-5.990; Cover pipe orifice actual diameter is 6.010-6.018, and its difference is 26-28 μ m.Thereby the annular gap area that forms between piston and cover pipe orifice is respectively 18956 μ m
2(Φ 3) and 56803 μ m
2(Φ 6).And the bore of micropin tip is 4-5 μ m during intracytoplasmic sperm injection, and sectional area is 20 μ m
2, the bore of DNA injection needle point is 2 μ m, sectional area only is 3.14 μ m
2The gap of syringe is respectively 6000 times (Φ 3mm syringe/Φ 2 μ m transgenic pins) and 2800 times (φ 6mm syringe/Φ 5 μ m monosperm pins) of micropin pipe area by contrast, because white oil is that oily matter has the good penetration performance, based on hydromechanical principle, pressure will transmit to reach syringe internal pressure poised state to the short position of distance.So when handwheel rotates Δ φ, medium when the pressure that is produced makes Δ ν is from the fit clearance leakage and cause the not timely pressure that obtains in micro needlepoint place of far-end to inject to drive material, the susceptiveness and the elaboration of microinjection instrument are affected, this because the systematic error that the machining accuracy of parts own is caused is to have a major defect of the syringe of structure as shown in Figure 1.Can observe the outwards phenomenon of wriggling of white oil pressurized tailing edge tube wall during practical operation, prove absolutely its leakage seriousness to sensitivity and precision influence when carrying out micro-injection.The microinjector of this structure is charging and is being also will constantly the gas that remains in the syringe be discharged from the telescopic head of micropin the operation of the medium that is sucking that telescopic head sucks from micropin during additional hydraulic medium.Therefore the operation that charges medium is very inconvenient, needs to improve.
The purpose of this utility model provides a kind of two-chamber and injects the range microinjection instrument more, the defective that the hydraulic medium that the fit clearance existence causes between piston and piston sleeve in the time of can not only overcoming the existing procucts injection leaks, and have higher injection precision and multiple injection measurement range selection, have the operating characteristics that charges hydraulic medium fast and get rid of gas in the hydraulic medium.
The purpose of this utility model is achieved in that the three-way body that a transparent organic glass material is made is attached on the horizontal component of a square frame, a horizontal component and a support link, the horizontal vestibule of three-way body is connected with a polyfluortetraethylene pipe, the other end of polyfluortetraethylene pipe is connected with the micropin pipe, and the telescopic other end of micropin is installed the microinjection needle tubing.On the vertical vestibule of three-way body, an exhaust pition is set with the helicitic texture installation, exhaust pition has the leakage that hermetically-sealed construction can reach the caused hydraulic medium of fit clearance when eliminating the reciprocal precession of piston, directly charge hydraulic transmission medium from vertical vestibule, make three-way body, polyfluortetraethylene pipe, micropin sleeve pipe and micropin form hydraulic system, the positive/negative pressure that produces during exhaust pition with rotation is got rid of the gas of bringing hydraulic medium when changing micropin into or is sucked the hydraulic medium of a spot of hydraulic medium loss when changing micropin to replenish from the micropin sleeve opening, another stomidium at the horizontal vestibule of three-way body has been installed another microinjection piston with a hermetically-sealed construction, hermetically-sealed construction also is to eliminate the leakage that fit clearance causes, the inner hole thread of the other end of microinjection piston and two threaded screw rods links, outer first threaded portion of two threaded screw rods and a hold-down nut that is contained in the frame vertical component are connected, and pass wherein with a scale handwheel and link, rotate handwheel and can drive two threaded screw rods along its axis precession and drive the reciprocal precession of microinjection piston and carry out microinjection, susceptiveness and the elaboration that spring is injected with raising with the gap of eliminating microinjection piston thread and two threaded screw rod inner hole threads compressed in endoporus installation one at two threaded screw rods, and outer the filling of the thread pitch of microinjection piston and two threaded screw rods has the poor of 0.25mm/ commentaries on classics between thread pitch.Form differential screw drive structure, reaching with this is the purpose of high injection elaboration.Hold-down nut is linking to each other with screw on the frame vertical component along its external screw thread of fluting on the threaded portion of axis direction, suitable thread pitch error when tightening hold-down nut and can eliminating the reciprocal precession of pair lead screw, improve mechanically operated susceptiveness and elaboration purpose to reach, threaded portion at the microinjection piston is installed a synchronous nut, the microinjection piston drove in differential screw drive mode and does reciprocal precession when this nut was in free state, the two threaded screw rods of this nut rotation direction and when fastening with it the microinjection piston change screw rod into and directly drive, the precession amount of microinjection piston is determined with the outer first pitch of two threaded screw rods, the rotation exhaust pition also can carry out microinjection, Gu this forms three kinds of injection measurement range selection.Adopt above-mentioned utility model organization plan to have following positive effect and performance:
One, the not good and coarse problem of injection volume of micro-injection susceptiveness that the leakage of transmission medium causes in the generation system in the time of getting rid of because of the piston precession effectively.Compare susceptiveness with existing similar products and accuracy is significantly improved.
Two, can change and charge transmission medium and can get rid of bubble in the medium apace quickly and easily, Practical Performance obviously improves.
Three, injection is meticulousr, can reach skin liter (pl) level, and can use the different injection range and the precision of different choice in field according to the user.
Four, adopt transparent material as the positive and negative living parts of cutting down output, be convenient to the user and check the working condition of piston and the sealing property of system at any time.
Below in conjunction with drawings and Examples this utility model is further specified.
Fig. 2 is a vertical section of the present utility model structural map.Micropin 1 is installed on the front end of micropin sleeve pipe 2 in the embodiment shown in Figure 2, is attached to three-way body 4 by teflon hose 3; Exhaust pition 5 is installed in helicitic texture in the vestibule 5-6 of three-way body, and exhaust pition is to be used to charge white oil 4-1 and get rid of the bubble of bringing into when changing micropin in the medium.Exhaust pition is by screw rod 5-1, plug 5-2; O-ring seals 5-3; Pad 5-4; O-ring seals degree of opening adjusting nut 5-5 forms, earlier white oil is poured into the T type vestibule that vestibule 5-6 and sag pipe 2 make three-way body during use, complete full of liquid paraffin wax 4-1 in flexible pipe and the micropin pipe-in-pipe 2, form the hydraulic drive path, screw on big piston 5 then, regulate 5-5 to make O type circle be subjected to the inclined-plane 5-7 compression of plug 5-2 head and distortion and radially extend to closely to contact and form gap-free sealed state with vestibule 5-6, the moving range of exhaust pition is in bright finish section 5-6 all the time during use, the function of exhaust pition is to get rid of bubble apace and charge hydraulic medium, microinjection piston 6 is by O-ring seals 8-1, and pad 8-2 and nut 8 are installed in the vestibule 6-1.Suitably screw and be in gap-free sealed state when nut 8 can make 6 precession of microinjection piston all the time.The screw portion of piston 6 is installed in the internal thread hole of two threaded screw rods 7, being installed on the hold-doun nut 11 of screw mandrel 7.The pitch of piston 6 is that 0.75mm/ changes; The external screw thread pitch of screw mandrel 7 is that 1mm/ changes, when handwheel 9 drives screw mandrel 7 rotations one circle, microinjection piston 6 precession 0.25mm, piston 6, screw mandrel 7, handwheel 9, hold-down nut 11 forms differential reducing mechanism, in synchronous its effect of nut 7-1 of installing on microinjection piston 6 threaded portions is when synchronous nut 7-1 being threaded to screw mandrel 7 and can realizing that piston 6 and 7 is synchronized with the movement when fastening with it, the precession of this state lower piston 6 is that 1mm/ changes, for eliminating the fit tolerance gap of 11 of screw mandrel 7 and hold-down nuts, nut 11 can suitably be tightened, owing on the hold-down nut 11 groove that parallels to the axis is arranged, make it can strain and hold screw mandrel 7 tightly, thereby eliminate the gap of screw mandrel 7 and hold-down nut 11.Compression spring 7-2 pushes piston 6 to front end to eliminate the gap of microinjection piston 6 and screw mandrel 7 inner hole threads.The injection that the precession gap, front and back of microinjection piston 6 caused when the positive and negative rotation of handwheel had been eliminated in above-mentioned two interstitial structure measures that disappear record quick property and elaboration is affected may.It is whole that said structure is installed on frame 10 and the support 12 organizator by three-way body and hold-down nut 11.
The diameter of microinjection piston 6 is 1 two kinds of φ 4 and φ, and the outer unit of handwheel is φ 60mm.200 evenly divided lattice of graduation on first outside face, every lattice width is 0.9mm, 1.8 ° of angle-resolved Δ α, it all is only that such structure is to use operating characteristics still to read graduated comfortableness.When the handwheel external diameter surpasses 75-80mm, the control ability when user rotates handwheel will descend.Equally when the width of scale lattice during less than 0.8mm, reading graduated labor intensity will increase.When practical operation, user is read graduation range more than 30-35cm, eye differentiates an angle and reaches 9 ', the angle of human eye the best strive degree of the distinguishing limit 〉=3 '
[5]When considering actual use the restriction of the degree of fatigue of human eye and lighting condition get 9 ' more suitable.
When using φ 1mm microinjection, the injection range is that 196nl/ changes 980pl/ lattice (handwheel).But the in fact wide or also graduation of angle of 1.8 ° of lattice of the lattice of 0.9mm, the sensitivity limit of staff rotation hand wheel can reach 10 ' (on the handwheel of φ 60)
[6], so the calibration of this utility model handwheel is reliably conservative, the injection volume and the injection precision that are produced when table 1 is listed the piston of selecting different-diameter for use with different precession amount.
Table 1 piston diameter mm handwheel precession amount mm/ changes injection volume/tuberculosis ejaculation degree/lattice φ 4 0.25 3.14 μ l 15.7nl φ 41 12.6 μ l 62nl φ 1 0.25 196nl 980pl φ 11 785nl 3.9nl
Three-way body 4 usefulness transparent organic glass are made, and can observe the sealing state of each piston among vestibule 5-6 and the 6-4 easily.Can first pressure testing be in sealing state in use to guarantee piston; method is: sleeve pipe 2 front ends are immersed in the white oil; inhour is rotated piston 5 about 1/4 commentaries on classics and produce negative pressure in medium; if found to have bubble to occur near between sealing ring and medium would illustrate sealing ring to open degree not enough; adjusting nut 5-5 or 8; strengthen the Zhang Du of O type circle, to improve sealing property.Strengthen the Zhang Du of O type circle, to improve sealing property.
List of references
[1] Lu Shengdong.The modern molecular biology experimental technique, Higher Education Publishing House, 1993,13-14
[2] the former seven youths work of pipe, Zhang Zhi is super, and Xu Chunsheng translates, and mammal grows engineering experiment method, publishing house of Nanjing University, 1994,222-223
[3] Japanese Narishige Co Ltd.LVF General Catalog
[4] Chen Jilong, Ma Haifei, developmental biology progress, Higher Education Publishing House, 1994,225
[5] [ripple] W. Tai Lalinsiji work, just fair silk floss etc. is translated micromachine and precision instrument, China Machine Press, 1995,355-356
[6] Chen Guohua, instrument machinery and application thereof, Shanghai science tech publishing house, 1986.9
Claims (5)
1, a kind of two-chamber is injected the range microinjection instrument more, structure as shown in Figure 2, the three-way body (4) that transparent organic glass material is made is attached at the horizontal component of right angle frame (10), the horizontal component of frame and support (12) are connected, the horizontal vestibule (6-1) of three-way body (4) links with an end of a polyfluortetraethylene pipe (3), the other end is connected with micropin sleeve pipe (2), the telescopic other end of micropin is connected with microinjection pin (1), in three-way body (4), polyfluortetraethylene pipe (3), charge hydraulic medium (4-1) in micropin sleeve pipe (2) and microinjection pin (1) the binding path, it is characterized in that: go up with helicitic texture at the vertical vestibule (5-6) of three-way body (4) exhaust pition (5) is installed, horizontal vestibule (6-1) at three-way body (4) has been installed a microinjection piston (6) with the close structure of envelope, the inner bolt hole of the microinjection piston other end and two threaded screw rods (7) is connected, outer first threaded portion of two threaded screw rods be installed in frame (10) vertically in the hold-down nut (11) of part, link and pass wherein and be connected with scale handwheel (9), compression spring (7-2) has been installed in the endoporus of two threaded screw rods (7), synchronous nut (7-1) has been installed at the threaded portion of microinjection piston (6).
2, two-chamber according to claim 1 is injected the range microinjection instrument more, it is characterized in that: O RunddichtringO (5-3), pad (5-4) is installed in the head inclined-plane (5-7) of plug (5-2) successively and locates the endoporus that plug (5-2) passes screw rod (5-1), and the end of thread (5-2) that nut (5-5) is installed in plug constitutes exhaust pition (5).
3, two-chamber according to claim 1 is injected the micro-instrument of range more, it is characterized in that: microinjection piston (6) is by O-ring seals (8-1), pad (8-2), and the hermetically-sealed construction that nut (8) is constituted is installed on the horizontal vestibule (6-1) of three-way body (4).
4, two-chamber according to claim 1 is injected the range microinjection instrument more, the thread pitch that it is characterized in that microinjection piston (6) is that 0.75mm/ changes, outer first thread pitch of two threaded rods (7) is that 1mm/ changes, the scale of handwheel (9) is that graduation is 200 lattice/commentaries on classics, and the external diameter of handwheel (9) is 60mm.
5, two-chamber according to claim 1 is injected the range microinjection instrument more, it is characterized in that: hold-down nut (11) is slotted along axis direction at its threaded portion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99228317U CN2375316Y (en) | 1999-05-12 | 1999-05-12 | Double-cavity microsyringe with several injection ranges |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99228317U CN2375316Y (en) | 1999-05-12 | 1999-05-12 | Double-cavity microsyringe with several injection ranges |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2375316Y true CN2375316Y (en) | 2000-04-26 |
Family
ID=34017721
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN99228317U Expired - Fee Related CN2375316Y (en) | 1999-05-12 | 1999-05-12 | Double-cavity microsyringe with several injection ranges |
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| Country | Link |
|---|---|
| CN (1) | CN2375316Y (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100386055C (en) * | 2006-03-31 | 2008-05-07 | 西安交通大学 | Micro-injection and microring electrode combined probe and manufacturing method thereof |
| CN102573960A (en) * | 2009-09-30 | 2012-07-11 | 赛诺菲-安万特德国有限公司 | Drive mechanism for a drug delivery device |
| CN105420099A (en) * | 2015-12-31 | 2016-03-23 | 河海大学常州校区 | Efficient microinjection device |
| CN106148165A (en) * | 2016-07-21 | 2016-11-23 | 河海大学常州校区 | Micro-fluidic chip, injecting systems and method of work thereof for cell injection |
| CN114034225A (en) * | 2021-11-25 | 2022-02-11 | 广州市华粤行医疗科技有限公司 | Method for testing movement precision of injection needle under microscope |
-
1999
- 1999-05-12 CN CN99228317U patent/CN2375316Y/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100386055C (en) * | 2006-03-31 | 2008-05-07 | 西安交通大学 | Micro-injection and microring electrode combined probe and manufacturing method thereof |
| CN102573960A (en) * | 2009-09-30 | 2012-07-11 | 赛诺菲-安万特德国有限公司 | Drive mechanism for a drug delivery device |
| CN102573960B (en) * | 2009-09-30 | 2014-12-10 | 赛诺菲-安万特德国有限公司 | Drive mechanism for a drug delivery device |
| CN105420099A (en) * | 2015-12-31 | 2016-03-23 | 河海大学常州校区 | Efficient microinjection device |
| CN106148165A (en) * | 2016-07-21 | 2016-11-23 | 河海大学常州校区 | Micro-fluidic chip, injecting systems and method of work thereof for cell injection |
| CN106148165B (en) * | 2016-07-21 | 2019-04-12 | 河海大学常州校区 | Micro-fluidic chip, injecting systems and its working method for cell injection |
| CN114034225A (en) * | 2021-11-25 | 2022-02-11 | 广州市华粤行医疗科技有限公司 | Method for testing movement precision of injection needle under microscope |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |