CN113973822A - High-efficiency fruit coloring agent and preparation method thereof - Google Patents

High-efficiency fruit coloring agent and preparation method thereof Download PDF

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Publication number
CN113973822A
CN113973822A CN202010731251.2A CN202010731251A CN113973822A CN 113973822 A CN113973822 A CN 113973822A CN 202010731251 A CN202010731251 A CN 202010731251A CN 113973822 A CN113973822 A CN 113973822A
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polyoxyethylene
fruit
acid
sorbitan
coloring agent
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Chinese (zh)
Inventor
吴桐
梁晓
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Zhengzhou Nietzsche Biotechnology Co ltd
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Zhengzhou Nietzsche Biotechnology Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N33/00Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
    • A01N33/02Amines; Quaternary ammonium compounds
    • A01N33/12Quaternary ammonium compounds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The embodiment of the invention relates to the technical field of plant growth regulators, in particular to a high-efficiency fruit coloring agent which comprises the following components in percentage by weight: 1-50% of propyl dihydrojasmonate, 1-50% of choline bitartrate, 1-50% of methionine, 1-50% of EDDHA-Fe 1-50%, 1-10% of acid-base regulator, 1-80% of emulsifier and the balance of solvent. The embodiment of the invention has the beneficial effects that: the fruit inducing colorant can promote rapid coloring of fruit, increase sugar degree, and improve fruit quality; by promoting photosynthesis of plants, dry substance accumulation in fruits is promoted, fruit fullness is improved, and soft fruits are prevented.

Description

High-efficiency fruit coloring agent and preparation method thereof
Technical Field
The invention relates to the technical field of plant growth regulators, and particularly relates to a high-efficiency fruit coloring agent and a preparation method thereof.
Background
The dihydrojasmonic acid propyl ester can effectively promote synthesis and early expression of abscisic acid and ethylene in plants, because ethylene is a key substance for promoting fruit coloring, a market product directly sprayed with ethephon is available on the market, ethylene-like components are applied from an external source to achieve a coloring effect, but adverse effects such as soft fruit, fruit falling, fruit intolerance and storage intolerance are easy to occur due to strict requirements on the pesticide application technology, so that the use is strictly limited, in addition, the existing common coloring agent has low coloring efficiency, the fruit can not be promoted by promoting the filling degree of the fruit, and the storage property of the fruit is poor.
Disclosure of Invention
In order to solve the above problems, the embodiment of the present invention provides an efficient fruit colorant, which comprises the following components by weight: 1-50% of propyl dihydrojasmonate, 1-50% of choline bitartrate, 1-50% of methionine, 1-50% of EDDHA-Fe 1-50%, 1-10% of acid-base regulator, 1-80% of emulsifier and the balance of solvent.
Preferably, the composition comprises the following components in percentage by weight: 2-10% of propyl dihydrojasmonate, 2-10% of choline bitartrate, 1-5% of methionine, 1-5% of EDDHA-Fe 1-5%, 1-10% of acid-base regulator, 20-50% of emulsifier and the balance of solvent.
Preferably, the emulsifier is at least one of sorbitan monopalmitate, sorbitan fatty acid ester, castor oil polyoxyethylene ether, polyoxyethylene dioleate, polyoxyethylene (20EO) sorbitan monostearate, polyoxyethylene (20EO) sorbitan monopalmitate, tetraethylene glycol monolaurate, triethanolamine oleate, sorbitan laurate, polyoxyethylene fatty alcohol, polyoxyethylene (20EO) sorbitan monooleate, polyoxyethylene (20EO) oleyl ether, polyoxyethylene sorbitan trioleate, polyoxyethylene (20EO) sorbitan monolaurate, polyoxyethylene (4 EO) sorbitan monolaurate, polyoxyethylene cetyl ether, polyoxyethylene monostearate, and alkyl glycoside.
Preferably, the emulsifier is at least one of castor oil polyoxyethylene ether, polyoxyethylene (20EO) sorbitan monostearate, polyoxyethylene (20EO) sorbitan monooleate, polyoxyethylene (20EO) oleyl ether, polyoxyethylene (20EO) sorbitan monolaurate and alkyl glycoside.
Preferably, the weight percentage of the solvent is 1% -50%.
Preferably, the solvent is one or more of methanol, ethanol, water, glycerol, ethylene glycol, propylene glycol, acetone, glycerol, butanone, n-propanol and isopropanol.
Preferably, the solvent is 1-3 of water, methanol, ethanol, ethylene glycol, n-propanol and isopropanol.
Preferably, the acid-base modifier comprises an alkaline modifier and an acidic modifier, the alkaline modifier comprises one or more of sodium hydroxide, potassium hydroxide, calcium hydroxide, sodium carbonate, potassium pyrosulfate and sodium bicarbonate, and the acidic modifier comprises one or more of organic acids of citric acid, tartaric acid, salicylic acid, fumaric acid, malic acid and acetic acid.
Preferably, the alkaline regulator comprises 1-2 of sodium hydroxide and potassium pyrosulfate, and the acidic regulator comprises 1-2 of citric acid, tartaric acid and salicylic acid.
Preferably, the preparation method of the high-efficiency fruit coloring agent comprises the following steps:
step one, mixing propyl dihydrojasmonate with a solvent, adding an emulsifier, chelating and stirring at the temperature of 20-100 ℃, and chelating for 2-6 hours to obtain a solution I;
dissolving an alkaline regulator in water, and adding methionine, choline bitartrate and EHHDA-Fe to obtain a solution II;
dissolving an acid regulator in a solvent to obtain a solution III;
and step four, mixing the solution I in the step one with the solution III in the step three under the condition of slow stirring, carrying out heat release treatment, slowly adding the solution II after fully stirring and standing for 2-4 hours, and stirring and standing to obtain the high-efficiency fruit coloring agent.
The embodiment of the invention has the beneficial effects that:
the dihydrojasmonic acid propyl ester (PDJ) is a derivative of plant endogenous hormone jasmonic acid, the endogenous hormone jasmonic acid as a plant stress-resistant substance can be quickly transmitted and expressed in a plant body and release a synthesis signal for promoting the synthesis and early expression of plant endogenous ethylene and abscisic acid, and the ethylene as a necessary precursor for plant fruit coloring can promote the apparent appearance of fruit coloring when reaching a certain amount;
the method is characterized in that the propyl dihydrojasmonate is sprayed by an external source, and aims to provide a plant endogenous jasmonic acid analogue, so that the mature period of plant fruits is shortened, the stress response of crops is improved, nutrition is absorbed quickly, sufficient dry matter accumulation is provided for the fruits, and the increase and accumulation of soluble sugar in the fruits are promoted; the exogenous spraying of the dihydrojasmonic acid propyl ester can simultaneously promote the synthesis and the early expression of endogenous abscisic acid and ethylene and promote the accumulation of soluble sugar in fruits, thereby effectively promoting the natural color change and ripening of the fruits and having obvious advantages;
ethylene is used as a necessary endogenous hormone precursor in the coloring process, soluble sugar is accumulated to be used as a necessary nutrition precursor in the coloring process, and the ethylene and the soluble sugar are cooperated, so that the coloring of fruits can be more natural, the fruits are more excellent, the sugar-acid ratio is higher, and the storage resistance is stronger.
EDDHA-Fe provides iron ions which can be efficiently utilized for plant growth, and iron is a component of cytochrome and non-heme ferritin in photosynthesis, biological nitrogen fixation and respiration;
the choline bitartrate is a novel choline compound, has high choline content and stable character, has strong oxidation resistance, can effectively promote plant photosynthesis and dry matter accumulation, improves the filling degree of fruits and prevents soft fruits;
methionine is a single amino acid, is a precursor substance for plant ethylene synthesis, and can provide a sufficient precursor substance source for the synthesis of ethylene in the fruit coloring period;
the fruit inducing colorant composition has the advantages of stability, long lasting period, stable effect, no ethephon, no threshing, no leaf drop and other side effects; the fruit coloring agent can promote the fruit to be colored quickly, increase the sugar degree and improve the fruit quality; the photosynthesis of plants is promoted, so that the accumulation of dry substances in fruits, particularly the accumulation of sugar in the fruits, is promoted, and the color conversion is promoted; the efficient fruit coloring agent which is prepared by combining the substances can achieve the effects of quick coloring, fruit filling, fruit sugar-acid ratio increase, fruit increase, storage resistance improvement and the like.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The efficient fruit coloring agent comprises the following components in percentage by weight: 2.5% of propyl dihydrojasmonate, 3% of choline bitartrate, 0.5% of methionine, 2.5% of EDDHA-Fe2.5%, 4% of an acid-base regulator, 29.5% of an emulsifier and the balance of a solvent. Mixing the propyl dihydrojasmonate with a solvent, adding an emulsifier, chelating and stirring at the temperature of 20-100 ℃, and chelating for 2-6 hours to obtain a solution I; then dissolving methionine in an alkaline aqueous solution, and adding choline bitartrate and EHHDA-Fe to obtain a solution II; then dissolving an acid regulator by using a solvent to form a solution III; and finally, mixing the solution I and the solution III under the condition of slow stirring, slowly adding the solution II after fully stirring and standing for 2-4 hours, well stirring for heat release treatment, and naturally cooling to obtain the high-efficiency fruit coloring agent.
Example 2
The efficient fruit coloring agent comprises the following components in percentage by weight: the efficient fruit coloring agent comprises the following components in percentage by weight: 5% of propyl dihydrojasmonate, 4% of choline bitartrate, 0.8% of methionine, EDDHA-Fe3%, 6% of an acid-base regulator, 36% of an emulsifier and the balance of a solvent. Mixing the propyl dihydrojasmonate with a solvent, adding an emulsifier, chelating and stirring at the temperature of 20-100 ℃, and chelating for 2-6 hours to obtain a solution I; then dissolving methionine in an alkaline aqueous solution, and adding choline bitartrate and EHHDA-Fe to obtain a solution II; then dissolving an acid regulator by using a solvent to form a solution III; and finally, mixing the solution I and the solution III under the condition of slow stirring, slowly adding the solution II after fully stirring and standing for 2-4 hours, well stirring for heat release treatment, and naturally cooling to obtain the high-efficiency fruit coloring agent.
Example 3
The efficient fruit coloring agent comprises the following components in percentage by weight: the efficient fruit coloring agent comprises the following components in percentage by weight: 10% of propyl dihydrojasmonate, 9% of choline bitartrate, 1.2% of methionine, EDDHA-Fe6%, 10% of an acid-base regulator, 44% of an emulsifier and the balance of a solvent. Mixing the propyl dihydrojasmonate with a solvent, adding an emulsifier, chelating and stirring at the temperature of 20-100 ℃, and chelating for 2-6 hours to obtain a solution I; then dissolving methionine in an alkaline aqueous solution, and adding choline bitartrate and EHHDA-Fe to obtain a solution II; then dissolving an acid regulator by using a solvent to form a solution III; and finally, mixing the solution I and the solution III under the condition of slow stirring, slowly adding the solution II after fully stirring and standing for 2-4 hours, well stirring for heat release treatment, and naturally cooling to obtain the high-efficiency fruit coloring agent.
Comparative experiment
To further examine the actual effect of the fruit colorants of the present invention on crops, the inventors conducted further comparative experiments on crops.
(1) Test object
The experimental land is arranged in a certain apple orchard in Zhengzhou, the experimental terrain is flat, the fertility is moderate and uniform, the organic matter content of the soil is 12.1g/kg, and the pH = 7.2. The apple trees which grow uniformly in the growth period of the same crop are equally divided into 5 areas, 100 fruit trees in each area are respectively sprayed once by using the coloring agent of the embodiment 1, the coloring agent of the embodiment 2, the coloring agent of the embodiment 3 and a 40% ethephon aqua under the condition of avoiding direct sunlight 45 days and 30 days before the picking of the apples, and the condition that a layer of water mist is formed on the surface layer of the leaf surface but does not drop is taken as a standard. The remaining area was used as a control, which was treated with clean water as well. During harvesting, 100 apples were randomly harvested in each area, and the fruit coloring degree, soluble sugar content, and organic acid content were measured, and the results are shown in tables 1, 2, and 3.
The color index calculation formula is that the color index (%) is (Σ number of fruit pieces per stage × representative value per stage)/(total number of pieces × highest representative value) × 100%.
Figure DEST_PATH_IMAGE001
Table 1: comparative Table of the Effect of the fruit inducing colorant composition of the present invention on apple coloring
Figure DEST_PATH_IMAGE002
Table 2: data are the average of 10 determinations
As can be seen from tables 1 and 2, the coloring agents of examples 1 to 3 all had better effect on apple coloring than the control group and also better effect than the single group of 40% ethephon solution; the color index of apples treated with the colorants of examples 1-3 increased from more than 60% to more than 90%. The product can effectively promote the fruit coloring, and the experimental research shows that the fruit can be rapidly colored within one week by spraying at the early stage of the fruit coloring, so that the fruit can be brought into the market 7-9 days in advance.
Figure DEST_PATH_IMAGE003
The average value of 10 experiments for the measurement is shown in Table 3 when no numeral is specified
As can be seen from Table 3, the coloring agents of examples 1-3 all affected apples better than the control group and better than the single group; the content of soluble sugar in the apple fruits treated by the coloring agent in the embodiment 1-3 is improved by 11.82 percent to the maximum extent, and the content of organic acid is reduced by 23.08 percent to the maximum extent, so that the product can effectively increase the sugar degree of the apple, reduce the content of the organic acid in the fruits, improve the taste of the fruits and improve the quality of the fruits.
The photosynthesis test of the apple treated with the coloring agent of the present invention shows that the coloring agent can raise the physiological activity of crop and promote the growth of crop, and the photosynthesis strength can be raised by over 50%.
It should be noted that those skilled in the art can easily understand that the scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the invention, one skilled in the art can make equivalent changes or substitutions on the related technical features, and the technical solutions after the changes or substitutions will fall into the protection scope of the invention.

Claims (10)

1. The efficient fruit coloring agent is characterized by comprising the following components in percentage by weight: 1-50% of propyl dihydrojasmonate, 1-50% of choline bitartrate, 1-50% of methionine, 1-50% of EDDHA-Fe 1-50%, 1-10% of acid-base regulator, 1-80% of emulsifier and the balance of solvent.
2. The high-potency fruit colorant according to claim 1, comprising the following components in percentage by weight: 2-10% of propyl dihydrojasmonate, 2-10% of choline bitartrate, 1-5% of methionine, 1-5% of EDDHA-Fe 1-5%, 1-10% of acid-base regulator, 20-50% of emulsifier and the balance of solvent.
3. A high fruit coloring agent according to claim 1 or 2, the emulsifier is at least one of sorbitan monopalmitate, sorbitan fatty acid ester, castor oil polyoxyethylene ether, polyoxyethylene dioleate, polyoxyethylene (20EO) sorbitan monostearate, polyoxyethylene (20EO) sorbitan monopalmitate, tetraethylene glycol monolaurate, triethanolamine oleate, sorbitan laurate, polyoxyethylene fatty alcohol, polyoxyethylene (20EO) sorbitan monooleate, polyoxyethylene (20EO) oleyl ether, polyoxyethylene sorbitan trioleate, polyoxyethylene (20EO) sorbitan monolaurate, polyoxyethylene (4 EO) sorbitan monolaurate, polyoxyethylene cetyl ether, polyoxyethylene monostearate and alkyl glycoside.
4. A high performance fruit coloring agent according to claim 3, wherein said emulsifier is at least one selected from the group consisting of castor oil polyoxyethylene ether, polyoxyethylene (20EO) sorbitan monostearate, polyoxyethylene (20EO) sorbitan monooleate, polyoxyethylene (20EO) oleyl ether, polyoxyethylene (20EO) sorbitan monolaurate, and alkyl glycoside.
5. A high fruit efficiency colorant according to claim 1 or 2, wherein the solvent is present in an amount of 1% to 50% by weight.
6. A high performance fruit coloring agent according to claim 5, wherein said solvent is one or more of methanol, ethanol, water, glycerol, ethylene glycol, propylene glycol, acetone, glycerol, methyl ethyl ketone, n-propanol, and isopropanol.
7. The high-potency fruit colorant according to claim 6, wherein the solvent is 1-3 selected from the group consisting of water, methanol, ethanol, ethylene glycol, n-propanol, and isopropanol.
8. The high-potency fruit colorant according to claim 1 or 2, wherein the pH modifier comprises an alkaline modifier and an acidic modifier, the alkaline modifier comprises one or more of sodium hydroxide, potassium hydroxide, calcium hydroxide, sodium carbonate, potassium pyrosulfate, and sodium bicarbonate, and the acidic modifier comprises one or more organic acids selected from citric acid, tartaric acid, salicylic acid, fumaric acid, malic acid, and acetic acid.
9. The high-potency fruit colorant according to claim 8, wherein the alkaline modifier comprises 1-2 of sodium hydroxide and potassium pyrosulfate, and the acidic modifier comprises 1-2 of citric acid, tartaric acid and salicylic acid.
10. A method of producing a high fruit coloring agent according to any one of claims 1 to 9, comprising the steps of:
step one, mixing propyl dihydrojasmonate with a solvent, adding an emulsifier, chelating and stirring at the temperature of 20-100 ℃, and chelating for 2-6 hours to obtain a solution I;
dissolving an alkaline regulator in water, and adding methionine, choline bitartrate and EHHDA-Fe to obtain a solution II;
dissolving an acid regulator in a solvent to obtain a solution III;
and step four, mixing the solution I in the step one with the solution III in the step three under the condition of slow stirring, carrying out heat release treatment, slowly adding the solution II after fully stirring and standing for 2-4 hours, and stirring and standing to obtain the high-efficiency fruit coloring agent.
CN202010731251.2A 2020-07-27 2020-07-27 High-efficiency fruit coloring agent and preparation method thereof Pending CN113973822A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4309205A (en) * 1979-08-27 1982-01-05 The State Of Israel Ministry Of Agriculture Enhancement of reproductive plant development by means of choline salts
CN86104810A (en) * 1985-06-07 1987-03-04 三菱瓦斯化学株式会社 Promote the method for plant growing
CN101142925A (en) * 2007-11-02 2008-03-19 祖小力 Reverse-resisting yield-increasing preparation for plant
CN103609593A (en) * 2013-12-04 2014-03-05 河北省林业科学研究院 Agricultural composition with function of sugar increase and coloring of fruit
CN105961435A (en) * 2016-06-02 2016-09-28 郑州卓越农业科技有限公司 Fruit induction coloring agent composition

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4309205A (en) * 1979-08-27 1982-01-05 The State Of Israel Ministry Of Agriculture Enhancement of reproductive plant development by means of choline salts
CN86104810A (en) * 1985-06-07 1987-03-04 三菱瓦斯化学株式会社 Promote the method for plant growing
CN101142925A (en) * 2007-11-02 2008-03-19 祖小力 Reverse-resisting yield-increasing preparation for plant
CN103609593A (en) * 2013-12-04 2014-03-05 河北省林业科学研究院 Agricultural composition with function of sugar increase and coloring of fruit
CN105961435A (en) * 2016-06-02 2016-09-28 郑州卓越农业科技有限公司 Fruit induction coloring agent composition

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
何平等: "茉莉酸酯类对秋甜桃果实着色及品质的影响", 《分子植物育种》 *
党纳等: "新型苹果品质改良剂的作用效果研究", 《北方园艺》 *

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