CN113973701A - Method for producing pinellia ternata bulbil by water culture method - Google Patents
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Abstract
The invention discloses a method for producing pinellia ternata bulbil by a water culture method, aiming at the problems of harm of seed-borne diseases in the production process of pinellia ternata seedlings, overhigh propagation cost of tissue culture seedlings and the like, the following technical scheme is established: firstly, a pinellia ternata clump seedling, namely a clumped sterile tissue culture seedling, is obtained by utilizing traditional tissue culture or plant bioreactor propagation; secondly, transferring the seedlings to rock wool and other water culture carriers for culture on the basis of hardening off the clustered seedlings, and forming guarantee conditions for the development of bulbils by promoting the vegetative growth in the early growth stage; in the development stage of the bulbil, a good foundation is laid for the growth and development of the bulbil by applying a microbial agent and improving the proportion of P/K fertilizer; and finally, promoting the transfer of the nutritive organs to propagules such as bulbels and the like through methyl jasmonate and the like and high-temperature water control treatment. The preparation method is simple, the process is controllable, and the prepared pinellia ternata bulbels are large and uniform in size, can be used for seedling breeding and can also be used as production seeds.
Description
Technical Field
The invention belongs to the technical field of agricultural biology, and particularly relates to a method for producing pinellia ternata bulbil by a water culture method.
Background
Pinellia tuber [ pinellia tuber ], [ solution of pinellia tuber ]Pinelliaternata (Thunb.) Briet ]The pinellia ternate is also called as pinellia ternata or pinellia ternata, is a herbal medicinal plant of Araceae, is a high-frequency used traditional Chinese medicinal material, and is propagated mainly through tubers, bulbils and seeds; plant tissue culture is also applied to pinellia ternata germplasm resource excavation and seedling rapid propagation as a modern biotechnology, and a corresponding breeding system is established (Chengji Shuang, Jiaming Liang, Ouyangkai, pinellia ternata biological resource and cell engineering, science publishing Co., 2013.). However, the conventional production method mainly involving tuber breeding has the following disadvantages. Firstly, the propagation coefficient is low, and the production is 1: 3-6/year in general; secondly, the seeds are transported and planted in the field for a long time, so that soil-borne diseases and seed-borne diseases are cross-polluted, and germplasm resources are disordered; thirdly, the plant tissue culture can improve the propagation efficiency and realize the purification of germplasm resources, but the period from the propagation mode of tissue culture seedlings to the acquisition of production seeds is long, and the economic benefit is low. The limitation links and the planting risks of pinellia ternate production are long-term seed adjustment and large-area high-density planting, which cause disease outbreaks such as bacterial rot and fungal damping-off, and even cause large-area production reduction and top-off. The production of sterile seeds can reduce the threat of seed-borne diseases to a great extent, but a production technical scheme matched with the production technical scheme is needed, and the biotechnological advantage of tissue culture is brought into play, so that the production method is effectively applied to production practice. To solve the above problems, many attempts have been made by the former.
CN202110201618.4 belongs to the field of seedling culture and relates to a method and a device for water culture high-yield seedling culture of pinellia ternata. The water culture method disclosed by the invention has the advantages that under the controllable environmental condition, illumination and nutrient solution circulation are automatically controlled, and the three-dimensional multilayer planting and efficient breeding of pinellia ternata seedlings are realized. However, in this document, only a method of producing pinellia tubers by planting in a hydroponic planting basket was attempted; the water culture scheme of the pinellia ternata effectively avoids the influence of soil-borne pathogens on the growth of the pinellia ternata; however, the invention uses the seed stems in the production, 2 percent carbendazim powder suspension is adopted to soak the seed stems for 15 minutes, and sodium hypochlorite or soap water is used for cleaning and sterilization, so as to reduce pathogenic bacteria carried by the seed stems. Both bacterial rot and fungal damping-off are infectious diseases, and bactericides and the like are difficult to completely permeate into pinellia tuber seed stems, so that the sterilization is not complete in the cleaning process, and cross contamination is easy to form.
Because the bulbels are produced by asexual propagation and do not directly contact with soil, aseptic seedlings can be more easily obtained compared with seed stems, and the development degrees of the bulbels are synchronous, so that the bulbels are more suitable for being used as breeders or protospecies compared with seed stems with different development periods. For example, CN 111226798B discloses a method for directly obtaining pinellia ternata bulbil by tissue culture, which comprises taking leaves of pinellia ternata aseptic seedling as explant, inoculating into bulbil induction medium, and inducing with multiple plant growth regulators to make the leaves of pinellia ternata in vitro generate bulbil. The produced bulbils can grow into plantlets through substrate cultivation. However, tests prove that the formation of the bulbels by the method completely depends on the nutrition of the culture medium, and the value of the accumulation of plant photosynthesis products is not exerted, so that the bulbels generated by the tissue culture are small in size, limited in quantity and not suitable for productive application.
Seed propagation is also a way of wild pinellia tuber, CN110679417a discloses a method for propagating seeds of pinellia tuber; the method is valuable for screening excellent individual plants; CN110036851B also discloses a water-dry segmented seed breeding method for pinellia ternata, which comprises picking incompletely mature pinellia ternata seeds or mature pinellia ternata seeds, carrying out pinellia ternata floating seedling culture on Chinese medicine residues or perlite substrates, and then transplanting the seeds into a field, wherein the used propagules are not aseptic seedlings; CN110679417A also describes a breeding method of pinellia ternata seeds, which differs from CN109105194A in that mature pinellia ternata seeds are picked, cultivated by a matrix cultivation method after being stored in winter, and transplanted. Seed propagation methods all illustrate the importance and advantages of producing sterile (virus-free) seedlings from seeds. However, since pinellia ternata is a polyploid species, progeny are severely differentiated, the yield of pinellia ternata seeds is small, and the conditions for producing seeds (such as the need for perennial large tubers) are not easy to control, so that pinellia ternata is not suitable for production.
In the case of sufficient water supply, pinellia tends to maintain vegetative growth without falling seedlings, which facilitates the formation of larger tubers, seeds and bulblets. CN 112790031A discloses a method and a device for water culture high-yield seedling culture of pinellia ternata, which realize the collection of pinellia ternata seed stems or seeds from seeds in a planting basket through the design of the device, and collect tubers after water culture growth. The method emphasizes the control value of rhizoma Pinelliae hydroponics and facility cultivation on diseases, and can also improve production efficiency. However, the main propagation mode of pinellia ternata is bulbil, the method does not emphasize the growth promotion and collection of bulbil, and meanwhile, the method makes disinfectants such as sodium hypochlorite and soapy water unfavorable to the growth of pinellia ternata, cannot effectively remove viruses, and is not good for selecting tissue culture seedlings. CN 109804914B discloses a pinellia ternate soilless floating seedling raising method, which comprises the steps of supporting by using a sponge mat, selecting disinfected and sterilized pinellia ternate tubers for raising seedlings, transplanting the seedlings to a field after the seedlings are grown, and completing a medicinal material production period. This method emphasizes the control of soil-borne diseases by substrate cultivation, but it is difficult to completely remove tubers that have been contaminated with pathogenic bacteria as propagules by soaking disinfection, even causing cross-contamination during soaking.
Based on the bottleneck problem of the shortage of pinellia tuber seeds in the current industrial application, a new technical scheme is urgently needed to be developed to meet the production requirement of pinellia tuber seedlings.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for producing a large amount of pinellia ternata bulbil by a water culture method, and the practice proves that the bulbil can be used as a more efficient mode: each tuber with the size of 1cm can generate 3-5 leaves in a 50-day growth cycle under the normal production condition, and each leaf can generate at least 1 bulbil; each cluster of seedlings can generate 25 leaves in a growth period of 50 days, namely more than 25 bulbels and 4-6 expanded tubers are formed. The aseptic (and virus-free) seedlings are selected for moisturizing culture, so that the method is favorable for controlling diseases and prolonging the growing period of the pinellia ternata, realizes tuber expansion and mass production of propagules such as bulbels and the like, and has remarkable advantages.
In order to solve the problems of the prior art, the invention adopts the technical scheme that:
a method for producing pinellia ternata bulbil by a water culture method comprises the following steps:
step 1, obtaining pinellia ternata seedlings; step 2, hardening seedlings of pinellia ternata seedlings; step 3, water culture and bulbil growth promotion of pinellia ternata seedlings; step 4, collecting and storing mature pinellia ternata bulbels; and 5, utilizing the pinellia ternata bulbil.
In a modification, the pinellia ternata clump seedling in the step 1 is pinellia ternata clump bud with the characteristics of clump tubers; the method for obtaining the pinellia ternata clump seedlings in the step 1 is a clump aseptic seedling obtained by adopting a traditional plant tissue culture method, and comprises the following specific steps:
firstly, pinellia tuber petiole or immature seed is used as an explant, and a sterile explant is obtained through explant disinfection treatment;
secondly, inoculating the sterile explant into a pinellia ternata cluster bud culture medium for continuous culture for 30-40 days until vigorous cluster buds grow;
thirdly, cutting the induced cluster buds, transferring the cut cluster buds to a new pinellia ternata cluster bud culture medium, culturing for 25-60 days to form pinellia ternata cluster seedlings, judging whether the seedling hardening stage is suitable or not according to the growth condition of the cluster seedlings or directly entering a water culture stage for culturing when the cluster seedlings are continuously cultured to be strong enough to obtain bulbels; wherein, the clump seedlings entering the seedling hardening stage are provided with more than 3 formed native tubers or more than 5 leaves, and when the clump seedlings are strong enough, the clump seedlings have the state of 10 or more complete leaves.
The further improvement is that the pinellia ternata cluster buds growing vigorously in the second step can be transferred to a new cluster bud culture medium, one round of transfer is carried out after 13-20 days of culture, a large amount of cluster buds and cluster seedlings are continuously obtained through propagation, and the next round of breeding of the cluster seedlings in the third step is carried out.
The improvement is that the hardening off of the pinellia ternata clump seedlings in the step 2 adopts a seedling culture medium cultivation method, and concretely, the pinellia ternata clump seedlings are placed in a seedling culture medium and grow to form clumped pinellia ternata clump seedlings at the temperature of 18-30 ℃, and each clumped pinellia ternata clump seedling has more than 10 complete leaves; in the seedling hardening process, 1/1000-1/6000 urea, Huabao or N/P/K compound fertilizer are sprayed, and natural illumination or artificial light source is adopted; the seedling exercising time is 15-75 days.
The improvement is that the step 3 of water culture of the pinellia ternata clump seedlings means that the clump seedlings qualified in hardening seedlings are transplanted into a water culture device in a whole, and the seedlings are cultured for 35-110 days at 18-35 ℃ under natural illumination or artificial light source, and the qualified water culture effect is that more than 15 complete leaves grow out of each clump seedling and an expanded bulbil is formed; the culture solution used for culturing is a solution which is prepared by dissolving Huabao or an N/P/K compound fertilizer and then diluting the solution by 1-1000 times so as to preserve moisture and provide nutrients; the method for promoting the growth of the bulbels comprises the following steps: the first step, the semi-summer clump seedlings are irrigated with bacillus amyloliquefaciens 25 to 35 days before the pearl buds are harvestedBacillus amyloliquefaciens) Culturing for 1-2 times; and secondly, spraying monopotassium phosphate 1 for 15-20 days before the pearl buds are harvested: spraying 650 parts of solution at an interval of 1-2 days for 3 times continuously at a ratio of 250-1; thirdly, spraying methyl jasmonate solution with the concentration of 1/1000-1/3000 for 1-2 times 7-10 days before the harvest of the bulblets; and fourthly, increasing the water culture temperature and stopping watering 5 to 6 days before the pearl buds are harvested so as to promote the pearl buds to mature and completely pour the seedlings.
The further improvement is that in the step 3, the water culture device is a seedling tray, a seedling basket or a seedling pool containing a support; the water culture seedling support is formed by mixing one or more of rock wool, sponge, seedling substrate or flower mud.
The improvement is that the collection of the mature pinellia ternata bulbels in the step 4 is to collect the mature pinellia ternata bulbels, retain tubers of the bases for continuous culture, simultaneously replace the hydroponic liquid, add urea or a nitrogen-phosphorus-potassium compound fertilizer, and continue the culture to promote the generation of new leaves and bulbels; the preservation refers to drying the collected mature pinellia tuber bulbels to remove surface moisture, covering in fine sand, keeping the relative humidity at 20-35% and the temperature at 4-15 ℃, and keeping away from light; the preservation time of the bulbil is 12-18 months, and the bulbil can be used as a sterile original seed or a production seed of pinellia ternata.
As an improvement, the utilization of the pinellia ternata bulbil in the step 5 means that when the bulbil is used as a production seed in the cultivation process, a seedling culture substrate or nutrient soil is adopted for covering, and the covering thickness is 1.5-3.5 times of the diameter of the bulbil.
Has the advantages that:
compared with the prior art, the method for producing the pinellia ternata bulbil by the water culture method firstly obtains sterile (and virus-free) clustered seedlings by adopting modern biotechnology means such as tissue culture and the like, and provides standardized planting management to ensure the quantity and quality of bulbil formation and ensure the large-scale production of the pinellia ternata sterile seedlings. The method effectively solves the problem that a large amount of expanded bulbils are obtained by utilizing sterile clustered seedlings, the expanded bulbils do not carry specific pathogenic bacteria (such as bacterial soft rot, fungal damping-off and the like), and the growth periods are basically consistent; the bulbil obtained by the invention can be continuously propagated by original seeds and can also be used as raw medicinal materials for producing pinellia ternata by planting production seeds; as the effect of the invention, the ratio of the bulbil and the tuber of pinellia ternata obtained by each cluster seedling is mostly 6: 1-25: 1, in some cases, 50:1 or more can be obtained.
Drawings
FIG. 1 is a flow chart of water culture of high-yield tuber pinellia bulblet by clumping seedlings.
FIG. 2 is a diagram showing the effect of the culture process of pinellia tuber bulbil, wherein (A) the clumped seedlings obtained by tissue culture; (B) hardening off the cluster seedlings; (C) water culture of cluster seedlings; (D) and (5) a bulbil harvesting stage.
FIG. 3 shows the buds (right) and tubers (left) of pinellia ternata obtained by hydroponics.
FIG. 4 is the effect diagram of the process of forming bulbil by directly cultivating the cluster seedling of pinellia ternata, (A) transplanting for 20 days (the initial stage of vegetative growth); (B) transplanting for 55 days (mature pearl bud).
Detailed Description
The flow scheme used in example 1 of the present invention is shown in FIG. 1.
The pinellia ternata clump seedling is pinellia ternata clump bud with clump tuber characteristics, as shown in figure 2-A.
Example 1
The method comprises the steps of taking peony leaf type pinellia ternate and obtaining pinellia ternate cluster buds by a method of a reference document (Chengjishuang, Jiaming good, Ouyangkai, pinellia ternate biological resource and cell engineering, science publishing Co., Ltd 2013.), wherein a pinellia ternate cluster bud induction culture medium comprises Ms +6-BA 1.0 mg/L + NAA 0.02 mg/L + sucrose 30 g/L + agar 6.0 g/L, and the pH value is 5.8. Using pinellia ternata petiole as an explant, and performing explant disinfection and sterile water cleaning treatment to obtain a sterile petiole explant, wherein the cutting length of the petiole is 0.3-0.5 cm; transferring the cluster buds to a new pinellia tuber cluster bud culture medium for amplification culture after the cluster buds are formed, and continuously subculturing for 20 generations; and (3) continuously culturing the vigorously growing cluster buds for 30 days to obtain vigorously growing cluster seedlings of the pinellia ternata, wherein the obtained cluster seedlings have 3-7 formed native tubers and more than 5 complete leaves, and the process is in accordance with the seedling hardening stage.
Then, the pinellia ternata clump seedlings are placed in a new seedling culture substrate (the seedling culture substrate refers to CN 201911395941.9), the lower substrate is 5cm, the humidity is kept at 60-90%, and the growth temperature is 22-28 ℃. During the seedling hardening process, 1/1000-1/600 urea and Huabao are sprayed at intervals in the first 10 days. Natural illumination or artificial light source is adopted; hardening the seedlings for 25 days to obtain the pinellia ternata seedlings with cluster growth.
The water culture of the pinellia tuber tufted seedlings is a key link, the pinellia tuber seedlings growing in clusters are transplanted into carriers such as water-cultured rock wool and the like, new agricultural rock wool is soaked in tap water for more than 24 hours to remove redundant surface water, and then the new agricultural rock wool is placed in a seedling culture pond, and the rock wool is moisturized by 1000 times of diluent of N/P/K compound fertilizer. Culturing at 22-25 deg.C under natural illumination for 55 days. Pouring the Bacillus amyloliquefaciens culture solution for 2 times (the used Bacillus amyloliquefaciens culture is NB culture medium (10 g/L peptone, 3 g/L beef extract and 5 g/L sodium chloride) for fermentation culture of bacterial solution for 48-72 hrs) 30 days before the collection of the bulbils; spraying monopotassium phosphate 1 every other day 15 days before the harvest of the bulbels: 500 times of the solution for 3 times; spraying methyl jasmonate solution for 2 times every other day from 8 days before the harvesting of the bulbels, wherein the concentration of the methyl jasmonate is 1/3000; stopping watering 5 days before the bead buds are harvested; then collecting and grading mature pinellia ternata bulbels; meanwhile, tubers formed by the clumped seedlings are collected. Counting to obtain the size of the bulbil which is 0.41 plus or minus 0.27 cm, and the ratio of the bulbil to the tuber is 100: 7; as shown in fig. 3.
Separately storing the collected pinellia tuber bulbil and tuber in fine sand, keeping a certain dryness, and using as original seeds in the next year; the propagation coefficients of the harvested bulbels and tubers in the next growing season (3-6 months) respectively reach 1: 13 (w/w) and 1:7 (w/w), showing vigorous breeding of the resulting beads.
Example 2
Taking a peach leaf type pinellia ternate single plant, culturing by a reference method (Chenggangshuang, Jiaming liang, Ouyangkai, pinellia ternate biological resource and cell engineering, science publisher 2013.) to obtain pinellia ternate cluster buds, and continuously culturing for 40 days to obtain cluster seedlings suitable for cultivation; each cluster seedling has 5-7 complete leaves on average;
after hardening off, transplanting the seedlings to rock wool, culturing the seedlings in a greenhouse at the temperature of 22-30 ℃ for 20 days under the natural illumination condition, wherein the transplanting survival rate is close to 100 percent, and beginning to form bulbels (shown in figure 4 (A)); continuously culturing at 25-35 deg.C for 20 days, and spraying potassium dihydrogen phosphate 1: 350 solution, 3 times continuously; spraying methyl jasmonate solution with the concentration of 1/2000 for 2 times every other day from the 10 th day; stopping watering 5 days before the pearl buds are harvested so as to promote the pearl buds to mature and completely pour seedlings; and then collecting mature pinellia ternata bulbels, and basically pouring seedlings of the plants after the bulbels are rapidly expanded, wherein the average size of the bulbels is 0.61 +/-0.14 cm (figure 4 (B)), and the bulbels are full and have cultivation value.
Example 3
Taking a peach leaf type pinellia ternata single plant, culturing by a method of a reference document (Chenggangshuang, Jiaming liang, Ouyangkai, pinellia ternata biological resource and cell engineering, science publishing company 2013.) to obtain pinellia ternata cluster buds, and continuously culturing for 40 days to obtain pinellia ternata cluster seedlings suitable for cultivation;
the seedling exercising step refers to the operation of example 1;
hardening seedlings, transplanting the seedlings into flower mud for water culture, culturing the seedlings in a greenhouse at the temperature of between 22 and 30 ℃ for 55 days under the natural illumination condition, and forming mature pearl buds by the treatment according to the example 1; then collecting mature bulblets, keeping tubers of the base parts for continuous culture, simultaneously replacing hydroponic culture liquid, supplementing nitrogen, phosphorus and potassium compound fertilizer every other day, and continuously culturing to promote tubers to generate new leaves and bulblets; in the growth stage of the bulbels, monopotassium phosphate is added to promote the bulbels to expand; continuously culturing and collecting the bulbels for 4 times in one year, wherein the number of the bulbels generated in each cluster reaches 60-90; collecting the bulbils for use as production seeds.
The above description is only a preferred embodiment of the present invention, and the scope of the present invention is not limited thereto, and any simple modifications or equivalent substitutions of the technical solutions that can be obviously obtained by those skilled in the art within the technical scope of the present invention are within the scope of the present invention.
Claims (8)
1. A method for producing pinellia ternata bulbil by a water culture method is characterized by comprising the following steps:
step 1, obtaining pinellia ternata seedlings; step 2, hardening seedlings of pinellia ternata seedlings; step 3, water culture and bulbil growth promotion of pinellia ternata seedlings; step 4, collecting and storing mature pinellia ternata bulbels; and 5, utilizing the pinellia ternata bulbil.
2. The method for producing pinellia ternata bulbil by a hydroponic method according to claim 1, wherein the pinellia ternata seedling in step 1 is a pinellia ternata bud with tuber characteristics; the method for obtaining the pinellia ternata clump seedlings in the step 1 is a clump aseptic seedling obtained by adopting a traditional plant tissue culture method, and comprises the following specific steps:
firstly, pinellia tuber petiole or immature seed is used as an explant, and a sterile explant is obtained through explant disinfection treatment;
secondly, inoculating the sterile explant into a pinellia ternata cluster bud culture medium for continuous culture for 30-40 days until vigorous cluster buds grow;
thirdly, cutting the induced cluster buds, transferring the cut cluster buds to a new pinellia ternata cluster bud culture medium, culturing for 25-60 days to form pinellia ternata cluster seedlings, judging whether the seedling hardening stage is suitable or not according to the growth condition of the cluster seedlings or directly entering a water culture stage for culturing when the cluster seedlings are continuously cultured to be strong enough to obtain bulbels; wherein, the clump seedlings entering the seedling hardening stage are provided with more than 3 formed native tubers or more than 5 leaves, and when the clump seedlings are strong enough, the clump seedlings have the state of 10 or more complete leaves.
3. The method for producing pinellia ternata bulblet by hydroponics as claimed in claim 2, wherein the vigorous pinellia ternata cluster buds in the second step can be transferred to a new cluster bud culture medium, and after culturing for 13-20 days, one round is transferred, and a large number of cluster buds and cluster seedlings are continuously obtained by propagation, and then the breeding of the cluster seedlings in the third step of the next round is carried out.
4. The method for producing pinellia ternata bulbil through a hydroponic method according to claim 1, wherein the hardening of seedlings of the pinellia ternata clump seedlings in the step 2 is implemented through a seedling culture substrate cultivation method, specifically, the pinellia ternata clump seedlings are placed in a seedling culture substrate and grow to form clustered pinellia ternata clump seedlings at the temperature of 18-30 ℃, and each clustered pinellia ternata clump seedling has more than 10 complete leaves; in the seedling hardening process, 1/1000-1/6000 urea, Huabao or N/P/K compound fertilizer are sprayed, and natural illumination or artificial light source is adopted; the seedling exercising time is 15-75 days.
5. The method for producing pinellia ternata bulbil by a hydroponic method according to claim 1, wherein the step 3 of hydroponics of the pinellia ternata clump seedlings is that the clumps of qualified acclimatized seedlings are transplanted into a hydroponic device in a whole manner and cultured for 35-110 days at 18-35 ℃ under natural illumination or artificial light source, and the qualified hydroponics effect is that more than 15 complete leaves grow out of each clump of qualified seedlings and expanded bulbels are formed; the culture solution used for culturing is a solution which is prepared by dissolving Huabao or an N/P/K compound fertilizer and then diluting the solution by 1-1000 times so as to preserve moisture and provide nutrients; the method for promoting the growth of the bulbels comprises the following steps: the first step, the semi-summer clump seedlings are irrigated with bacillus amyloliquefaciens 25 to 35 days before the pearl buds are harvestedBacillus amyloliquefaciens) Culturing for 1-2 times; and secondly, spraying monopotassium phosphate 1 for 15-20 days before the pearl buds are harvested: spraying 650 parts of solution at an interval of 1-2 days for 3 times continuously at a ratio of 250-1; thirdly, spraying methyl jasmonate solution with the concentration of 1/1000-1/3000 for 1-2 times 7-10 days before the harvest of the bulblets; and fourthly, increasing the water culture temperature and stopping watering 5 to 6 days before the pearl buds are harvested so as to promote the pearl buds to mature and completely pour the seedlings.
6. The method for producing pinellia ternata bulbil through the hydroponics method according to claim 5, wherein in the step 3, the hydroponics device is a seedling tray, a seedling basket or a seedling pool containing a support; the water culture seedling support is formed by mixing one or more of rock wool, sponge, seedling substrate or flower mud.
7. The method for producing pinellia ternata bulbil through the hydroponics method according to claim 1, wherein the collection of the mature pinellia ternata bulbil in the step 4 is to collect the mature pinellia ternata bulbil, retain tubers of the base part for continuous culture, replace the hydroponics solution, add urea or nitrogen-phosphorus-potassium compound fertilizer, continue the culture and promote the production of new leaves and bulbil; the preservation refers to drying the collected mature pinellia tuber bulbels to remove surface moisture, covering in fine sand, keeping the relative humidity at 20-35% and the temperature at 4-15 ℃, and keeping away from light; the preservation time of the bulbil is 12-18 months, and the bulbil can be used as a sterile original seed or a production seed of pinellia ternata.
8. The method for producing pinellia ternata bulbil by a hydroponic method according to claim 1, wherein the pinellia ternata bulbil in the step 5 is covered by a seedling culture medium or nutrient soil during the cultivation process when the pinellia ternata bulbil is used as a production seed, and the covering thickness is 1.5-3.5 times of the diameter of the pinellia ternata bulbil.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1799342A (en) * | 2005-12-30 | 2006-07-12 | 浙江省农业科学院 | Pinellia detoxification, tissue culture and quick propagation method |
| CN102150624A (en) * | 2011-04-29 | 2011-08-17 | 南京工业大学 | Tissue culture and rapid propagation method of pinellia genus plant |
| CN103109688A (en) * | 2013-02-06 | 2013-05-22 | 淮北师范大学 | Method for delaying sprout tumbling at high temperature and raising yield and alkaloid content of pinellia ternate |
| JP2014150771A (en) * | 2013-02-12 | 2014-08-25 | Sumitomo Electric Ind Ltd | Medicinal plant cultivation method |
| CN110278871A (en) * | 2019-07-01 | 2019-09-27 | 长江大学 | Using one step of Jing Banxia tissue culture tufted seedling at the tissue culture method of kind |
| CN110278870A (en) * | 2019-07-01 | 2019-09-27 | 长江大学 | Utilize the tissue culture method with leaf petiole forming seedling through one step culture of Jing Banxia tissue culture tufted seedling |
| CN111226798A (en) * | 2020-04-15 | 2020-06-05 | 广西壮族自治区农业科学院 | Method for inducing bulbil by pinellia ternata leaf |
| CN112790031A (en) * | 2021-02-23 | 2021-05-14 | 中国中药有限公司 | Method and device for water culture high-yield seedling culture of pinellia ternata |
| CN113481125A (en) * | 2021-07-12 | 2021-10-08 | 华中农业大学 | Bacillus amyloliquefaciens and application thereof in disease resistance and growth promotion of pinellia ternata |
-
2021
- 2021-10-25 CN CN202111239025.3A patent/CN113973701B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1799342A (en) * | 2005-12-30 | 2006-07-12 | 浙江省农业科学院 | Pinellia detoxification, tissue culture and quick propagation method |
| CN102150624A (en) * | 2011-04-29 | 2011-08-17 | 南京工业大学 | Tissue culture and rapid propagation method of pinellia genus plant |
| CN103109688A (en) * | 2013-02-06 | 2013-05-22 | 淮北师范大学 | Method for delaying sprout tumbling at high temperature and raising yield and alkaloid content of pinellia ternate |
| JP2014150771A (en) * | 2013-02-12 | 2014-08-25 | Sumitomo Electric Ind Ltd | Medicinal plant cultivation method |
| CN110278871A (en) * | 2019-07-01 | 2019-09-27 | 长江大学 | Using one step of Jing Banxia tissue culture tufted seedling at the tissue culture method of kind |
| CN110278870A (en) * | 2019-07-01 | 2019-09-27 | 长江大学 | Utilize the tissue culture method with leaf petiole forming seedling through one step culture of Jing Banxia tissue culture tufted seedling |
| CN111226798A (en) * | 2020-04-15 | 2020-06-05 | 广西壮族自治区农业科学院 | Method for inducing bulbil by pinellia ternata leaf |
| CN112790031A (en) * | 2021-02-23 | 2021-05-14 | 中国中药有限公司 | Method and device for water culture high-yield seedling culture of pinellia ternata |
| CN113481125A (en) * | 2021-07-12 | 2021-10-08 | 华中农业大学 | Bacillus amyloliquefaciens and application thereof in disease resistance and growth promotion of pinellia ternata |
Non-Patent Citations (1)
| Title |
|---|
| 李西文等: "半夏有性繁殖研究", 《现代中药研究与实践》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116762653A (en) * | 2023-07-27 | 2023-09-19 | 江苏农牧科技职业学院 | Method for rapidly obtaining pinellia ternate seedlings through seeds |
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