CN113966783A - Chinese herbal medicine additive for improving probiotic fermentation - Google Patents
Chinese herbal medicine additive for improving probiotic fermentation Download PDFInfo
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Abstract
The invention relates to a Chinese herbal medicine additive for improving probiotic fermentation, belonging to the technical field of feed additives, and the additive comprises the following raw materials in parts by weight: 20-30 parts of Chinese herbal medicine extract, 300 parts of astragalus polysaccharide 280-containing powder, 200 parts of codonopsis polysaccharide 180-containing powder, 5-10 parts of microcapsule probiotics, 100 parts of inulin, 10-15 parts of jerusalem artichoke extract and 3-5 parts of auxiliary agent; the additive is prepared by the following steps: mixing the raw materials uniformly, heating to 60-70 deg.C, stirring and mixing for 2-4h, transferring to a granulator for granulation, and drying at 50-60 deg.C for 24h to obtain Chinese herbal medicine additive for improving probiotic fermentation; the invention adopts the mutual benefit of probiotics and Chinese herbal medicines, on one hand, the probiotics promote the exertion of the drug effect of the Chinese herbal medicines and improve the palatability of the Chinese herbal medicines, and on the other hand, the Chinese herbal medicines promote the better metabolism of the probiotics and promote the growth of the raised animals.
Description
Technical Field
The invention belongs to the technical field of feed additives, and particularly relates to a Chinese herbal medicine additive for improving probiotic fermentation.
Background
In the process of animal breeding, the antibiotics can prevent and treat diseases and also can seriously damage the immune system of the animals, so that the animals lose the disease resistance, and the excessive administration can also cause the edible safety of animal products, thereby causing great threat to human health. Antibiotic substitutes largely used by animal feed production enterprises after banning resistance mainly comprise: probiotic preparation, complex enzyme preparation, acidifier, oligosaccharide, antibacterial peptide, saccharicterpenin, polysaccharide, Chinese herbal medicine and plant extract, and plant essential oil.
The probiotic preparation is mainly used for feed fermentation, so that a large amount of beneficial metabolites are generated, a large amount of live beneficial bacteria are contained, the pH value of intestinal tracts can be reduced, the growth of harmful pathogenic bacteria is inhibited, enteritis and diarrhea are prevented, the probiotic preparation and the intestinal tract bacteria are fermented in a combined manner generally, and the probiotic preparation contains a large amount of enzymes, so that the utilization rate of the feed can be improved, and the digestion and absorption of animals are promoted.
The existing probiotic preparation has the advantages of low activity, short shelf life, easy inactivation in animal intestinal tracts, single function, incapability of realizing the mutual benefits of Chinese herbal medicines and probiotics and small growth regulation effect on raised animals, so that the technical problem to be solved at present is to provide the Chinese herbal medicine additive for improving the fermentation of the probiotics.
Disclosure of Invention
The invention aims to provide a Chinese herbal medicine additive for improving probiotic fermentation, so as to solve the technical problems in the background technology.
The purpose of the invention can be realized by the following technical scheme:
a Chinese herbal medicine additive for improving probiotic fermentation comprises the following raw materials in parts by weight: 20-30 parts of Chinese herbal medicine extract, 300 parts of astragalus polysaccharide 280-containing powder, 200 parts of codonopsis polysaccharide 180-containing powder, 5-10 parts of microcapsule probiotics, 100 parts of inulin, 10-15 parts of jerusalem artichoke extract and 3-5 parts of auxiliary agent;
the Chinese herbal medicine additive for improving probiotic fermentation is prepared by the following steps:
adding astragalus polysaccharide, codonopsis pilosula polysaccharide, microcapsule probiotics, jerusalem artichoke extract, inulin and auxiliary agents into the Chinese herbal medicine extract, heating to 60-70 ℃, stirring and mixing for 2-4h, transferring to a granulator for granulation, and drying at 50-60 ℃ for 24h to obtain the Chinese herbal medicine additive for improving probiotic fermentation.
Further, the Chinese herbal medicine extract is prepared by the following steps:
step A1, preparing the following Chinese herbal medicine raw materials in parts by weight: 40-45 parts of daylily, 18-20 parts of salvia miltiorrhiza, 20-22 parts of codonopsis pilosula, 1-1.5 parts of angelica, 60-80 parts of ginseng rhizoma phragmitis, 10-15 parts of ligusticum wallichii and 40-50 parts of eucommia ulmoides;
step A2, drying the Chinese herbal medicine raw materials at 45 ℃ to constant weight, crushing by a crusher, and sieving by a 60-mesh sieve to obtain Chinese herbal medicine powder; choline chloride, citric acid and deionized water are mixed according to the proportion of 0.1 mol: 0.1 mol: 85-114mL, and stirring at 80 ℃ for 30-60min to obtain an extracting solution;
step A3, adding the extracting solution into a reaction kettle, and mixing the extracting solution according to the solid-liquid ratio of 1 g: adding 20mL of the Chinese herbal medicine powder into a reaction kettle, extracting for 15-25min under the condition of 60 ℃ microwave, then centrifuging for 5min at the rotation speed of 10000r/min, taking supernate, and filtering with a 0.22umPES (polyether sulfone) microporous membrane to obtain the Chinese herbal medicine extract.
The red sage root contains a large amount of tanshinone and has the function of delaying cell aging; the codonopsis pilosula contains alkaloid, saponin and protein, the ginseng reed head contains a large amount of ginsenoside, the Chinese herbal medicine extract can play the role of prebiotics, by changing certain nutrient components or growth conditions or being used as a substrate by the probiotics, the growth of the probiotics is promoted, moreover, the Chinese herbal medicine organic acid components have pH buffering effect, can maintain the stability of the pH of the intestinal tract, provide a suitable living environment for increasing the number of probiotics, compared with the prior Chinese herbal medicine extraction technology, the invention adopts the eutectic solvent to replace the traditional water-alcohol solution or organic solvent, overcomes the defects of low boiling point, easy volatilization, high toxicity, low affinity to enzyme and the like of the traditional organic solvent, and the citric acid is edible acid, can enhance normal metabolism in animal body, and choline chloride can prevent fat accumulation and tissue degeneration in liver and kidney, and promote amino acid recombination; the utilization rate of amino acid, especially the essential amino acid methionine in vivo is improved, so that the choline chloride is used as a hydrogen bond acceptor, and the citric acid is used as a hydrogen bond donor, and the method is more beneficial to the growth of the raised animals compared with other extraction solvents.
Further, the microcapsule probiotics are prepared by the following steps:
step B1, sterilizing the MRS culture medium and introducing N2To exclude 02Adding sodium thioglycollate to obtain an activated culture medium, inoculating probiotic strains into the activated culture medium in an anaerobic incubator, culturing until the 0D is 0.3-0.6, centrifuging, and suspending thalli in sterile deoxygenated physiological saline with the same volume to obtain a probiotic dispersion liquid;
step B2, mixing the skim milk powder and the probiotic dispersion liquid, placing the mixture into an extruder to be extruded into strips, discharging the strips, placing the strips into a container containing liquid nitrogen to be frozen, and then freezing and drying the strips for 48 hours at the temperature of minus 40 ℃ to obtain an intermediate product;
and step B3, adding trehalose and vegetable oil into a pre-prepared sodium caseinate solution with the mass fraction of 10%, controlling the temperature to be 40 ℃, dispersing for 3-5min at the rotating speed of 6000rpm, homogenizing for three times at the pressure of 3000psi by a high-pressure homogenizer, storing for 24h at the temperature of 0 ℃, adding an intermediate product, stirring for 10min at the rotating speed of 1000rpm, drying by a spray dryer at the speed of 5mL/min, collecting the dried product at the inlet temperature of 135-145 ℃, the outlet temperature of 80 ℃, and storing at the temperature of 4 ℃ to obtain the microcapsule probiotics.
Further, in the step B1, the amount of the sodium thioglycolate is 10-15% of the mass of the MRS culture medium, and the probiotics is one or more of streptococcus thermophilus, lactobacillus bulgaricus, bifidobacterium lactis, lactobacillus acidophilus, lactobacillus casei, bifidobacterium, lactobacillus brevis and lactobacillus plantarum which are mixed according to any proportion.
Further, the dosage ratio of the skim milk powder to the probiotic dispersion liquid in the step B2 is 800 g: 500-550 mL.
Further, the mass ratio of the trehalose, the vegetable oil, the sodium caseinate solution and the intermediate product in step B3 is 0.25-0.5: 0.25: 10: 2-2.5.
The high temperature resistance and the pH resistance of the probiotic strains are improved by embedding the probiotic strains in microcapsules, so that the probiotics can keep activity in the stomach of animals and play a role.
Further, the adjuvant is made by the following steps:
step C1, cleaning and crushing fresh and non-rotten purple sweet potatoes to obtain purple sweet potato pulp, adding 3.5-4.2% by mass of citric acid solution into the purple sweet potato pulp, stirring for 20-40min, adding cellulase, leaching for 2-4h at 50-55 ℃, cooling to room temperature, centrifuging at the rotation speed of 4000rpm for 10-12min, taking supernatant, and freeze-drying at the temperature of-40 ℃ to constant weight to obtain anthocyanin extract;
step C2, mixing the anthocyanin extract, the corn peptide, the vitamin C and the tea polyphenol according to the mass ratio of 0.5-0.8: 2-3: 4-5: 2.5, uniformly mixing to obtain the auxiliary agent.
Wherein the dosage ratio of the purple sweet potato pulp to the citric acid solution to the cellulase is 15 mL: 1mL of: 0.8-1.4 g.
The invention has the beneficial effects that:
the invention provides a Chinese herbal medicine additive for improving probiotic fermentation, which adopts mutual benefits of probiotics and Chinese herbal medicines, on one hand, the probiotics promote the exertion of the efficacy of the Chinese herbal medicines and improve the palatability of the Chinese herbal medicines, and on the other hand, the Chinese herbal medicines promote the better metabolism of the probiotics and promote the growth of raised animals. The additive disclosed by the invention has the beneficial effects that a small amount of microcapsule probiotics is added, and the high temperature resistance and pH resistance of the probiotics strain are improved by microencapsulating the probiotics strain, so that the activity of the probiotics strain can be kept in the stomach of an animal and the probiotics strain can play a role, most remarkably, the eutectic solvent is adopted to replace the traditional water-alcohol solution or organic solvent, the defects that the traditional organic solvent is low in boiling point, easy to volatilize, high in toxicity, low in enzyme affinity and the like are overcome, the citric acid is edible acid, the normal metabolism in the animal body can be enhanced, the choline chloride can prevent fat accumulation and tissue degeneration in the liver and kidney, and the recombination of amino acids can be promoted; the utilization rate of amino acid, especially the essential amino acid methionine in vivo is improved, so that the choline chloride is used as a hydrogen bond acceptor, and the citric acid is used as a hydrogen bond donor, and the method is more beneficial to the growth of the raised animals compared with other extraction solvents.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
This example provides a herbal extract, which is prepared by the following steps:
step A1, preparing the following Chinese herbal medicine raw materials in parts by weight: 40 parts of daylily, 18 parts of salvia miltiorrhiza, 20 parts of codonopsis pilosula, 1 part of angelica, 60 parts of ginseng rhizoma phragmitis, 10 parts of ligusticum wallichii and 40 parts of eucommia ulmoides;
step A2, drying the Chinese herbal medicine raw materials at 45 ℃ to constant weight, crushing by a crusher, and sieving by a 60-mesh sieve to obtain Chinese herbal medicine powder; choline chloride, citric acid and deionized water are mixed according to the proportion of 0.1 mol: 0.1 mol: 85mL of the mixture is mixed and stirred for 30min at 80 ℃ to obtain an extracting solution;
step A3, adding the extracting solution into a reaction kettle, and mixing the extracting solution according to the solid-liquid ratio of 1 g: adding 20mL of the Chinese herbal medicine powder into a reaction kettle, extracting for 15min under the condition of 60 ℃ microwave, then centrifuging for 5min at the rotating speed of 10000r/min, taking supernate, and filtering with a 0.22umPES (polyether sulfone) microporous membrane to obtain the Chinese herbal medicine extract.
Example 2
This example provides a herbal extract, which is prepared by the following steps:
step A1, preparing the following Chinese herbal medicine raw materials in parts by weight: 42 parts of daylily, 19 parts of salvia miltiorrhiza, 21 parts of codonopsis pilosula, 1.2 parts of angelica, 70 parts of ginseng rhizoma phragmitis, 12 parts of ligusticum wallichii and 45 parts of eucommia ulmoides;
step A2, drying the Chinese herbal medicine raw materials at 45 ℃ to constant weight, crushing by a crusher, and sieving by a 60-mesh sieve to obtain Chinese herbal medicine powder; choline chloride, citric acid and deionized water are mixed according to the proportion of 0.1 mol: 0.1 mol: mixing 95mL of the mixture, and stirring at 80 ℃ for 40min to obtain an extracting solution;
step A3, adding the extracting solution into a reaction kettle, and mixing the extracting solution according to the solid-liquid ratio of 1 g: adding 20mL of the Chinese herbal medicine powder into a reaction kettle, extracting for 18min under the condition of microwave at 60 ℃, then centrifuging for 5min at the rotating speed of 10000r/min, taking supernate, and filtering with a 0.22umPES (polyether sulfone) microporous membrane to obtain the Chinese herbal medicine extract.
Example 3
This example provides a herbal extract, which is prepared by the following steps:
step A1, preparing the following Chinese herbal medicine raw materials in parts by weight: 45 parts of daylily, 20 parts of salvia miltiorrhiza, 22 parts of codonopsis pilosula, 1.5 parts of angelica, 80 parts of ginseng rhizoma phragmitis, 15 parts of ligusticum wallichii and 50 parts of eucommia ulmoides;
step A2, drying the Chinese herbal medicine raw materials at 45 ℃ to constant weight, crushing by a crusher, and sieving by a 60-mesh sieve to obtain Chinese herbal medicine powder; choline chloride, citric acid and deionized water are mixed according to the proportion of 0.1 mol: 0.1 mol: 114mL of the mixture is mixed and stirred for 60min at 80 ℃ to obtain an extracting solution;
step A3, adding the extracting solution into a reaction kettle, and mixing the extracting solution according to the solid-liquid ratio of 1 g: adding 20mL of the Chinese herbal medicine powder into a reaction kettle, extracting for 25min under the condition of 60 ℃ microwave, then centrifuging for 5min at the rotating speed of 10000r/min, taking supernate, and filtering with a 0.22umPES (polyether sulfone) microporous membrane to obtain the Chinese herbal medicine extract.
Comparative example 1
The extract in example 1 was replaced with an ethanol solution with a mass fraction of 80%, and the remaining preparation steps were unchanged.
Example 4
This example provides a microcapsule probiotic, which is prepared by the following steps:
step B1, sterilizing the MRS culture medium and introducing N2To exclude 02Adding sodium thioglycollate to obtain an activated culture medium, inoculating probiotic strains into the activated culture medium in an anaerobic incubator, culturing until the 0D is 0.3, centrifuging, and suspending thalli in sterile deoxygenated physiological saline with the same volume to obtain a probiotic dispersion liquid, wherein the using amount of the sodium thioglycollate is MRS culture medium10% of the amount, wherein the probiotics are streptococcus thermophilus and lactobacillus bulgaricus according to the mass ratio of 1: 1, mixing;
step B2, mixing 800g of skim milk powder with 500mL of probiotic dispersion liquid, extruding the mixture into strips in an extruder, discharging the strips, freezing the strips in a container containing liquid nitrogen, and freeze-drying the strips for 48 hours at the temperature of-40 ℃ to obtain an intermediate product;
and step B3, adding 0.25g of trehalose and 0.25g of vegetable oil into 10g of a 10% sodium caseinate solution prepared in advance, controlling the temperature to be 40 ℃, dispersing for 3min at a rotating speed of 6000rpm, homogenizing for three times at 3000psi by a high-pressure homogenizer, storing for 24h at 0 ℃, adding 2g of an intermediate product, stirring for 10min at a rotating speed of 1000rpm, drying by a spray dryer at a speed of 5mL/min, collecting the dried product, and storing at 4 ℃ to obtain the microcapsule probiotics.
Example 5
This example provides a microcapsule probiotic, which is prepared by the following steps:
step B1, sterilizing the MRS culture medium and introducing N2To exclude 02Adding sodium thioglycollate to obtain an activated culture medium, inoculating probiotic strains into the activated culture medium in an anaerobic incubator, culturing until the D0 is 0.4, centrifuging, and suspending thalli in isometric sterile deoxygenated physiological saline to obtain a probiotic dispersion liquid, wherein the using amount of the sodium thioglycollate is 12% of the mass of the MRS culture medium, and the probiotics are streptococcus thermophilus and lactobacillus bulgaricus according to the mass ratio of 1: 1, mixing;
step B2, mixing 800g of skim milk powder with 520mL of probiotic dispersion liquid, extruding the mixture into strips in an extruder, discharging the strips, freezing the strips in a container containing liquid nitrogen, and freeze-drying the strips for 48 hours at the temperature of-40 ℃ to obtain an intermediate product;
and step B3, adding 0.30g of trehalose and 0.25g of vegetable oil into 10g of a 10% sodium caseinate solution prepared in advance, controlling the temperature to be 40 ℃, dispersing for 4min at a rotating speed of 6000rpm, homogenizing for three times at 3000psi by a high-pressure homogenizer, storing for 24h at 0 ℃, adding 2.2g of an intermediate product, stirring for 10min at a rotating speed of 1000rpm, drying by a spray dryer at a speed of 5mL/min, collecting the dried product, and storing at 4 ℃ to obtain the microcapsule probiotics.
Example 6
This example provides a microcapsule probiotic, which is prepared by the following steps:
step B1, sterilizing the MRS culture medium and introducing N2To exclude 02Adding sodium thioglycollate to obtain an activated culture medium, inoculating probiotic strains into the activated culture medium in an anaerobic incubator, culturing until the D0 is 0.6, centrifuging, and suspending thalli in isometric sterile deoxygenated physiological saline to obtain a probiotic dispersion liquid, wherein the using amount of the sodium thioglycollate is 15% of the mass of the MRS culture medium, and the probiotics are streptococcus thermophilus and lactobacillus bulgaricus according to the mass ratio of 1: 1, mixing;
step B2, mixing 800g of skim milk powder with 550mL of probiotic dispersion liquid, extruding the mixture into strips in an extruder, discharging the strips, freezing the strips in a container containing liquid nitrogen, and freeze-drying the strips for 48 hours at the temperature of-40 ℃ to obtain an intermediate product;
and step B3, adding 0.5g of trehalose and 0.25g of vegetable oil into 10g of a pre-prepared sodium caseinate solution with the mass fraction of 10%, controlling the temperature to be 40 ℃, dispersing for 5min at the rotating speed of 6000rpm, homogenizing for three times at the pressure of 3000psi by a high-pressure homogenizer, preserving for 24h at the temperature of 0 ℃, adding 2.5g of an intermediate product, stirring for 10min at the rotating speed of 1000rpm, drying by a spray dryer at the speed of 5mL/min, preserving at the inlet temperature of 145 ℃ and at the outlet temperature of 80 ℃, collecting the dried product, and preserving at the temperature of 4 ℃ to obtain the microcapsule probiotics.
Example 7
A Chinese herbal medicine additive for improving probiotic fermentation comprises the following raw materials in parts by weight: 20 parts of Chinese herbal medicine extract of example 1, 280 parts of astragalus polysaccharide, 180 parts of codonopsis pilosula polysaccharide, 5 parts of microcapsule probiotics of example 3, 100 parts of inulin, 10 parts of jerusalem artichoke extract and 3-5 parts of auxiliary agent;
the Chinese herbal medicine additive for improving probiotic fermentation is prepared by the following steps:
adding astragalus polysaccharide, codonopsis pilosula polysaccharide, microcapsule probiotics, jerusalem artichoke extract, inulin and auxiliary agents into the Chinese herbal medicine extract, heating to 60 ℃, stirring and mixing for 2 hours, transferring to a granulator for granulation, and drying at 50 ℃ for 24 hours to obtain the Chinese herbal medicine additive for improving probiotic fermentation.
Wherein, the auxiliary agent is prepared by the following steps:
step C1, cleaning and crushing fresh and non-rotten purple sweet potatoes to obtain purple sweet potato pulp, adding 1mL of citric acid solution with the mass fraction of 3.5% into 15mL of purple sweet potato pulp, stirring for 20min, adding 0.8g of cellulase, leaching for 2h at the temperature of 50 ℃, cooling to room temperature, centrifuging at the rotating speed of 4000rpm for 10min, taking supernate, and freeze-drying at the temperature of-40 ℃ to constant weight to obtain an anthocyanin extract;
step C2, mixing the anthocyanin extract, the corn peptide, the vitamin C and the tea polyphenol according to the mass ratio of 0.5: 2: 4: 2.5, uniformly mixing to obtain the auxiliary agent.
Example 8
A Chinese herbal medicine additive for improving probiotic fermentation comprises the following raw materials in parts by weight: 25 parts of Chinese herbal medicine extract of example 2, 285 parts of astragalus polysaccharide, 190 parts of codonopsis pilosula polysaccharide, 8 parts of microcapsule probiotics of example 4, 100 parts of inulin, 12 parts of jerusalem artichoke extract and 4 parts of auxiliary agent;
the Chinese herbal medicine additive for improving probiotic fermentation is prepared by the following steps:
adding astragalus polysaccharide, codonopsis pilosula polysaccharide, microcapsule probiotics, jerusalem artichoke extract, inulin and auxiliary agents into the Chinese herbal medicine extract, heating to 65 ℃, stirring and mixing for 3 hours, transferring to a granulator for granulation, and drying at 55 ℃ for 24 hours to obtain the Chinese herbal medicine additive for improving probiotic fermentation.
Wherein, the auxiliary agent is prepared by the following steps:
step C1, cleaning and crushing fresh and non-rotten purple sweet potatoes to obtain purple sweet potato pulp, adding 1mL of citric acid solution with the mass fraction of 3.8% into 15mL of purple sweet potato pulp, stirring for 30min, adding 1.1g of cellulase, leaching for 3h at the temperature of 52 ℃, cooling to room temperature, centrifuging at the rotating speed of 4000rpm for 11min, taking supernate, and freeze-drying at the temperature of-40 ℃ to constant weight to obtain an anthocyanin extract;
step C2, mixing the anthocyanin extract, the corn peptide, the vitamin C and the tea polyphenol according to the mass ratio of 0.6: 2.5: 4.5: 2.5, uniformly mixing to obtain the auxiliary agent.
Example 9
A Chinese herbal medicine additive for improving probiotic fermentation comprises the following raw materials in parts by weight: 30 parts of Chinese herbal medicine extract of example 3, 300 parts of astragalus polysaccharide, 200 parts of codonopsis pilosula polysaccharide, 10 parts of microcapsule probiotics of example 6, 100 parts of inulin, 15 parts of jerusalem artichoke extract and 5 parts of auxiliary agent;
the Chinese herbal medicine additive for improving probiotic fermentation is prepared by the following steps:
adding astragalus polysaccharide, codonopsis pilosula polysaccharide, microcapsule probiotics, jerusalem artichoke extract, inulin and auxiliary agents into the Chinese herbal medicine extract, heating to 70 ℃, stirring and mixing for 4 hours, transferring to a granulator for granulation, and drying at 60 ℃ for 24 hours to obtain the Chinese herbal medicine additive for improving probiotic fermentation.
Wherein, the auxiliary agent is prepared by the following steps:
step C1, cleaning and crushing fresh and non-rotten purple sweet potatoes to obtain purple sweet potato pulp, adding 1mL of citric acid solution with the mass fraction of 4.2% into 15mL of purple sweet potato pulp, stirring for 40min, adding 1.4g of cellulase, leaching for 4h at the temperature of 55 ℃, cooling to room temperature, centrifuging at the rotation speed of 4000rpm for 12min, taking supernate, and freeze-drying at the temperature of-40 ℃ to constant weight to obtain an anthocyanin extract;
step C2, mixing the anthocyanin extract, the corn peptide, the vitamin C and the tea polyphenol according to the mass ratio of 0.8: 3: 5: 2.5, uniformly mixing to obtain the auxiliary agent.
Comparative example 1
The herb extracts of example 7 were replaced with the herb extracts obtained in comparative example 1, and the remaining raw materials and preparation steps were unchanged.
Comparative example 2
The microcapsule probiotics in the example 8 are replaced by streptococcus thermophilus and lactobacillus bulgaricus in a mass ratio of 1: 1 the mixed strain, the other raw materials and the preparation process are not changed.
Comparative example 3
The adjuvant in example 9 was removed and the remaining raw materials and preparation process were unchanged.
The additives of examples 7 to 9 and comparative examples 1 to 3 were subjected to performance tests:
(I) nursery pig breeding experiment
180 pigs weighing 15kg are selected for the experiment, the average group is divided into 6 groups, the experimental period is 35 days, 2% of the additive of the example group is respectively added into the basic ration in the groups 7, 8 and 9, 2% of the additive of the comparative example is respectively added into the basic ration in the groups 1 to 3, and the pigs are fed freely. The feeding and growth conditions of the pigs are observed and recorded every day in the experimental period, the weight of the pigs is weighed at the end of the experiment, the daily weight gain, the daily feeding and the feed-meat ratio of the pigs in each experimental group are calculated, and the experimental results are shown in table 1:
TABLE 1
As can be seen from Table 1, the additives prepared in examples 7 to 9 can effectively promote the growth of animals, increase the daily feed intake, reduce the feed-meat ratio and improve the utilization rate of the feed.
(II) weaned piglet cultivation test
54 pigs with the weight of 10kg are selected for weaning, and are averagely divided into 6 groups, wherein the number of the male piglets is half that of the female piglets, and the experimental period is 60 days. Examples 7, 8, 9 groups each had 2% of the additive of the example group added to the basal diet, comparative examples 1-3 groups each had 2% of the additive of the comparative example added to the basal diet, live pigs were fed freely, the feeding and health status of piglets were observed and recorded every day during the experimental period, the weight was weighed at the end of the experiment, and the experimental results are shown in table 2:
TABLE 2
| Item | Daily gain (kg) | Daily ingestion (kg/head) | Meat ratio of materials | Diarrhea Rate (%) |
| Example 7 | 0.45 | 0.65 | 1.56 | 3.21 |
| Example 8 | 0.46 | 0.65 | 1.56 | 3.32 |
| Example 9 | 0.46 | 0.65 | 1.56 | 3.29 |
| Comparative example 1 | 0.41 | 0.43 | 1.64 | 7.84 |
| Comparative example 2 | 0.37 | 0.45 | 1.63 | 7.41 |
| Comparative example 3 | 0.38 | 0.41 | 1.65 | 8.17 |
From table 2, the piglets in examples 7-9 have higher daily gain, lower feed conversion ratio and lower diarrhea rate, which shows that the additive prepared by the invention can effectively promote the growth of the piglets and improve the breeding benefit.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing is illustrative and explanatory only and is not intended to be exhaustive or to limit the invention to the precise embodiments described, and various modifications, additions, and substitutions may be made by those skilled in the art without departing from the scope of the invention or exceeding the scope of the claims.
Claims (8)
1. The Chinese herbal medicine additive for improving probiotic fermentation is characterized by comprising the following raw materials in parts by weight: 20-30 parts of Chinese herbal medicine extract, 300 parts of astragalus polysaccharide 280-containing powder, 200 parts of codonopsis polysaccharide 180-containing powder, 5-10 parts of microcapsule probiotics, 100 parts of inulin, 10-15 parts of jerusalem artichoke extract and 3-5 parts of auxiliary agent;
wherein, the Chinese herbal medicine extract is prepared by the following steps:
step A1, drying and crushing the Chinese herbal medicines, and sieving the crushed Chinese herbal medicines with a 60-mesh sieve to obtain Chinese herbal medicine powder; choline chloride, citric acid and deionized water are mixed according to the proportion of 0.1 mol: 0.1 mol: 85-114mL, and stirring at 80 ℃ for 30-60min to obtain an extracting solution;
step A2, according to the solid-liquid ratio of 1 g: adding 20mL of Chinese herbal medicine powder into the extracting solution, extracting for 15-25min under the microwave condition of 60 ℃, centrifuging for 5min, taking supernatant, and filtering to obtain the Chinese herbal medicine extract.
2. The Chinese herbal medicine additive for improving probiotic fermentation according to claim 1, wherein the Chinese herbal medicine in the step A1 is prepared from the following Chinese herbal medicines in parts by weight:
40-45 parts of daylily, 18-20 parts of salvia miltiorrhiza, 20-22 parts of codonopsis pilosula, 1-1.5 parts of angelica, 60-80 parts of ginseng rhizoma phragmitis, 10-15 parts of ligusticum wallichii and 40-50 parts of eucommia ulmoides.
3. The Chinese herbal medicine additive for improving probiotic fermentation according to claim 1, wherein the microcapsule probiotic is prepared by the following steps:
step B1, sterilizing the MRS culture medium, activating with sodium thioglycolate, then inoculating probiotic strains, culturing until the 0D is 0.3-0.6, centrifuging, and re-suspending the strains to obtain a probiotic dispersion liquid;
step B2, mixing the skim milk powder and the probiotic dispersion liquid, extruding into strips, discharging, placing in a container containing liquid nitrogen for freezing, and then carrying out freeze drying at-40 ℃ for 48 hours to obtain an intermediate product;
and step B3, adding trehalose and vegetable oil into the sodium caseinate solution, controlling the temperature to be 40 ℃, dispersing for 3-5min, homogenizing for three times, storing at 0 ℃ for 24h, adding the intermediate product, stirring, performing spray drying, collecting the dried product, and storing at 4 ℃ to obtain the microcapsule probiotics.
4. The Chinese herbal medicine additive for improving probiotic fermentation according to claim 3, wherein the amount of sodium thioglycolate in step B1 is 10-15% of the mass of MRS medium.
5. The Chinese herbal medicine additive for improving probiotic fermentation according to claim 3, wherein the dosage ratio of the skim milk powder to the probiotic dispersion in the step B2 is 800 g: 500-550 mL.
6. The Chinese herbal medicine additive for improving probiotic fermentation according to claim 3, wherein the mass ratio of trehalose, vegetable oil, sodium caseinate solution and intermediate product in step B3 is 0.25-0.5: 0.25: 10: 2-2.5.
7. The Chinese herbal medicine additive for improving probiotic fermentation according to claim 1, characterized in that the adjuvant is prepared by the following steps:
step C1, cleaning and crushing purple sweet potatoes to obtain purple sweet potato pulp, adding a citric acid solution, stirring, adding cellulase, leaching at 50-55 ℃ for 2-4h, cooling, centrifuging, taking supernatant, and freeze-drying to constant weight to obtain an anthocyanin extract;
step C2, mixing the anthocyanin extract, the corn peptide, the vitamin C and the tea polyphenol according to the mass ratio of 0.5-0.8: 2-3: 4-5: 2.5, uniformly mixing to obtain the auxiliary agent.
8. The Chinese herbal medicine additive for improving probiotic fermentation according to claim 7, wherein the dosage ratio of the purple sweet potato pulp, the citric acid solution and the cellulase is 15 mL: 1mL of: 0.8-1.4 g.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115024417A (en) * | 2022-06-20 | 2022-09-09 | 北京都润科技有限公司 | Feed additive for fattening pigs as well as preparation method and application of feed additive |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130049118A (en) * | 2011-11-03 | 2013-05-13 | 경상북도 (관련부서:경상북도축산기술연구소장) | Maeksumseok mixed with herbs livestock feed additives and manufacturing method thereof, mixed feed additives and method for breeding livestock |
| CN103392946A (en) * | 2013-08-22 | 2013-11-20 | 广南(湛江)家丰饲料有限公司 | Pig feed additive capable of effectively improving immunity and intestinal health |
| CN104366147A (en) * | 2014-12-14 | 2015-02-25 | 河南联合英伟饲料有限公司 | Additive for piglet feed |
| CN105639124A (en) * | 2016-01-13 | 2016-06-08 | 河北省科学院生物研究所 | Microcapsule feed additive, and preparation method and application thereof |
| CN105639099A (en) * | 2015-12-30 | 2016-06-08 | 双胞胎（集团）股份有限公司 | Pig feed additive for improving intestinal health |
| CN106974107A (en) * | 2017-04-20 | 2017-07-25 | 合肥岭牧农产品有限公司 | Improve live pig immunity and improve the feed addictive of meat quality |
| CN108088943A (en) * | 2018-02-13 | 2018-05-29 | 沈阳药科大学 | Extractant-eutectic is dissolved in the application during effective component of chinese medicine measures |
| CN108850729A (en) * | 2018-08-30 | 2018-11-23 | 安徽金牧饲料有限公司 | One kind containing compound probiotic and Chinese herbal medicine feed for river crab |
| CN109602722A (en) * | 2018-12-30 | 2019-04-12 | 广东南芯医疗科技有限公司 | The coated probiotic microcapsule preparation of one primary yeast, preparation method and applications |
| CN110151795A (en) * | 2019-05-14 | 2019-08-23 | 广东大泽农生物科技股份有限公司 | A kind of clostridium butyricum active bacteria preparation and its production technology |
| CN111789911A (en) * | 2020-07-13 | 2020-10-20 | 珠海中科先进技术研究院有限公司 | A method for preparing aloe peel extract with eutectic solvent |
| AU2020103400A4 (en) * | 2020-11-11 | 2021-01-28 | Nanjing Forestry University | A fermented plant feed additive for weaned piglet |
| CN112868927A (en) * | 2021-02-01 | 2021-06-01 | 广东省农业科学院动物科学研究所 | Chinese herbal medicine and probiotic compound feed additive for relieving pig stress and improving meat quality and application thereof |
-
2021
- 2021-10-27 CN CN202111256848.7A patent/CN113966783A/en active Pending
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130049118A (en) * | 2011-11-03 | 2013-05-13 | 경상북도 (관련부서:경상북도축산기술연구소장) | Maeksumseok mixed with herbs livestock feed additives and manufacturing method thereof, mixed feed additives and method for breeding livestock |
| CN103392946A (en) * | 2013-08-22 | 2013-11-20 | 广南(湛江)家丰饲料有限公司 | Pig feed additive capable of effectively improving immunity and intestinal health |
| CN104366147A (en) * | 2014-12-14 | 2015-02-25 | 河南联合英伟饲料有限公司 | Additive for piglet feed |
| CN105639099A (en) * | 2015-12-30 | 2016-06-08 | 双胞胎（集团）股份有限公司 | Pig feed additive for improving intestinal health |
| CN105639124A (en) * | 2016-01-13 | 2016-06-08 | 河北省科学院生物研究所 | Microcapsule feed additive, and preparation method and application thereof |
| CN106974107A (en) * | 2017-04-20 | 2017-07-25 | 合肥岭牧农产品有限公司 | Improve live pig immunity and improve the feed addictive of meat quality |
| CN108088943A (en) * | 2018-02-13 | 2018-05-29 | 沈阳药科大学 | Extractant-eutectic is dissolved in the application during effective component of chinese medicine measures |
| CN108850729A (en) * | 2018-08-30 | 2018-11-23 | 安徽金牧饲料有限公司 | One kind containing compound probiotic and Chinese herbal medicine feed for river crab |
| CN109602722A (en) * | 2018-12-30 | 2019-04-12 | 广东南芯医疗科技有限公司 | The coated probiotic microcapsule preparation of one primary yeast, preparation method and applications |
| CN110151795A (en) * | 2019-05-14 | 2019-08-23 | 广东大泽农生物科技股份有限公司 | A kind of clostridium butyricum active bacteria preparation and its production technology |
| CN111789911A (en) * | 2020-07-13 | 2020-10-20 | 珠海中科先进技术研究院有限公司 | A method for preparing aloe peel extract with eutectic solvent |
| AU2020103400A4 (en) * | 2020-11-11 | 2021-01-28 | Nanjing Forestry University | A fermented plant feed additive for weaned piglet |
| CN112868927A (en) * | 2021-02-01 | 2021-06-01 | 广东省农业科学院动物科学研究所 | Chinese herbal medicine and probiotic compound feed additive for relieving pig stress and improving meat quality and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115024417A (en) * | 2022-06-20 | 2022-09-09 | 北京都润科技有限公司 | Feed additive for fattening pigs as well as preparation method and application of feed additive |
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