CN113943646A - Cancer cell sorting and detecting device for specific binding breast cancer cell research and development - Google Patents
Cancer cell sorting and detecting device for specific binding breast cancer cell research and development Download PDFInfo
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- 201000011510 cancer Diseases 0.000 title claims abstract description 70
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 69
- 206010006187 Breast cancer Diseases 0.000 title claims abstract description 17
- 208000026310 Breast neoplasm Diseases 0.000 title claims abstract description 17
- 238000012827 research and development Methods 0.000 title claims description 6
- 230000009870 specific binding Effects 0.000 title claims description 4
- 239000007788 liquid Substances 0.000 claims abstract description 68
- 238000001514 detection method Methods 0.000 claims abstract description 32
- 238000011160 research Methods 0.000 claims abstract description 3
- 238000007789 sealing Methods 0.000 claims description 14
- 230000027455 binding Effects 0.000 claims description 11
- 239000002699 waste material Substances 0.000 claims description 10
- 238000003780 insertion Methods 0.000 claims description 4
- 230000037431 insertion Effects 0.000 claims description 4
- 238000003466 welding Methods 0.000 claims description 4
- 230000000712 assembly Effects 0.000 claims description 2
- 238000000429 assembly Methods 0.000 claims description 2
- 238000000926 separation method Methods 0.000 abstract description 26
- 238000000034 method Methods 0.000 abstract description 19
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- 210000004027 cell Anatomy 0.000 description 50
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- 239000000243 solution Substances 0.000 description 22
- 210000000265 leukocyte Anatomy 0.000 description 12
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- 238000004140 cleaning Methods 0.000 description 3
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- 210000005259 peripheral blood Anatomy 0.000 description 3
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- 210000004881 tumor cell Anatomy 0.000 description 3
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Abstract
The invention discloses a cancer cell sorting and detecting device for research of specifically-combined breast cancer cells, and particularly relates to the technical field of cancer cell detection. According to the invention, the cancer cell solution in the liquid separating groove can be pumped into the separating groove again through the pump body for multiple separation, so that the concentration of the cancer cell solution can be effectively improved, the loss of the cancer cell solution can be effectively avoided in the process, and the concentration of the cancer cell solution can be rapidly adjusted.
Description
Technical Field
The invention relates to the technical field of cancer cell detection, in particular to a cancer cell sorting and detecting device for specifically combining breast cancer cell research.
Background
Cancer, also known as malignant tumor, is a disease caused by the malfunction of the mechanism controlling cell growth and proliferation. Malignant tumors grow rapidly and can destroy normal tissues and organs of the human body, and finally cause death of the patient. With the change of living habits and living environments of people, the current situation of cancer is more severe and becomes the most major problem affecting the public health in the world. Modern medicine finds that in the early stages of cancer recurrence and metastasis, tumor cells are shed from primary tumor foci into peripheral blood. Tumor cells (also called circulating tumor cells) in peripheral blood are often used for predicting survival of cancer patients, and can also be used for guiding cancer diagnosis and prognosis evaluation, so that a thought is provided for developing anti-cancer drugs. Therefore, the ability to rapidly and efficiently obtain tumor cells from peripheral blood would be of great significance for cancer diagnosis and treatment.
The invention patent of patent application publication No. CN112111385A discloses a cancer cell sorting and detecting device, which integrally completes the sorting and detection of cancer cells. The cancer cell sorting and detecting device provided by the embodiment of the invention sequentially comprises an upper clamp, a sorting unit, a detecting unit and a lower clamp from top to bottom, wherein the upper clamp is provided with a sample liquid inlet, and the lower clamp is provided with a detecting liquid outlet; the sample liquid inlet is communicated with the inlet of the sorting unit, the outlet of the sorting unit is communicated with the inlet of the detection unit, and the outlet of the detection unit is communicated with the detection liquid outlet. The cancer cell sorting and detecting device of the embodiment can separate cancer cells from a mixed solution of white blood cells and cancer cells, can detect the cancer cells quickly and accurately, can finish the sorting and detection of the cancer cells integrally, does not need to sort first, and can detect the cancer cells conveniently and quickly by using the detecting device.
However, when in actual use, the device needs to adjust the concentration of the sample liquid through the cooperation of a plurality of components, and in the process, the sample liquid needs to circulate inside a plurality of concentration channels, so that more sample liquid adheres to the inner wall of the concentration channel in the circulating process, the longer the path of the concentration channel is, the larger the loss of the sample liquid is, and the concentration of the sample liquid cannot be effectively adjusted. Therefore, a device for sorting and detecting cancer cells specifically binding to breast cancer cells is needed to solve the above problems.
Disclosure of Invention
In order to overcome the above drawbacks of the prior art, embodiments of the present invention provide a device for sorting and detecting cancer cells for research and development of specifically binding breast cancer cells, which can perform a circulating liquid separation operation on a sample solution through a circulating assembly, thereby adjusting the concentration of the sample solution and avoiding the loss of the sample solution, so as to effectively solve the above problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: the automatic sorting device comprises a cover plate and a shell arranged below the cover plate, wherein a round hole is formed in the top of the cover plate, a liquid inlet pipe is fixedly connected to the inner wall of the circumference of the round hole, a pressurizing assembly is arranged at the top of the cover plate, a sorting assembly and a detection assembly are respectively arranged in the shell, and a control panel is fixedly connected to the outer wall of one side of the shell;
the bottom fixedly connected with circulation subassembly of selecting separately the subassembly, the one end of circulation subassembly with select separately the subassembly intercommunication each other.
In a preferred embodiment, the pressurizing assembly comprises a fixed seat fixedly connected to the outer wall of the top of the cover plate, an air pump is fixedly connected to the outer wall of the top of the fixed seat, a connecting pipe is inserted into the outer wall of one side of the air pump, and one end of the connecting pipe is communicated with the liquid inlet pipe.
In a preferred embodiment, the top outer wall of the housing is provided with a sealing groove, the bottom outer wall of the cover plate is fixedly connected with a sealing clamping strip, the specification of the sealing clamping strip is matched with that of the sealing groove, and the outer walls of four sides of the housing are fixedly connected with positioning assemblies.
In a preferred embodiment, the positioning assembly includes fixing lugs fixedly connected to the cover plate and the four outer walls of the housing, threaded holes are formed in tops of the fixing lugs located on the four outer walls of the housing, insertion holes are formed in tops of the fixing lugs located on the four outer walls of the cover plate, fixing screws are threadedly connected to circumferential inner walls of the threaded holes, and one ends of the fixing screws penetrate through the insertion holes.
In a preferred embodiment, the sorting assembly comprises a first mounting plate fixedly connected to the inner wall of the shell, a sorting groove is fixedly connected to the outer wall of the top of the first mounting plate, the cross section of the sorting groove is spiral, the liquid inlet pipe is located right above one end of the sorting groove, a liquid separating groove and a waste liquid groove are fixedly connected to one end of the sorting groove, a sewage discharging hole is formed in the inner wall of the bottom of the waste liquid groove, and a turbulent flow assembly is arranged inside the sorting groove.
In a preferred embodiment, the circulation assembly comprises a liquid guide pipe fixedly connected to the outer wall of the bottom of the first mounting plate, a through hole is formed in the inner wall of the bottom of the liquid separation tank, the liquid guide pipe is communicated with the through hole, a first control valve is fixedly connected to the outer wall of one side of the liquid guide pipe, a liquid suction pipe is inserted into one side of the liquid guide pipe, a pump body is inserted into one end, away from the liquid guide pipe, of the liquid suction pipe, a fixed pipe is inserted into one side of the pump body, one end of the fixed pipe extends into the separation tank, and a second control valve is arranged on the outer wall of one side of the fixed pipe.
In a preferred embodiment, the inner walls of four sides of the shell are fixedly connected with second mounting plates, the outer walls of the tops of the second mounting plates are fixedly connected with shielding plates, the tops of the second mounting plates are provided with liquid discharge grooves, and the liquid discharge grooves and the through holes are located on the same vertical plane.
In a preferred embodiment, the detection assembly comprises a detection plate fixedly connected to the inner walls of the four sides of the casing, the detection plate is located below the second mounting plate, and a detection groove is formed in the top of the detection plate.
In a preferred embodiment, the vortex subassembly includes fixed connection and is in the connecting block of sorting groove one side inner wall, one side outer wall of connecting block rotates and is connected with the pivot, the pivot is kept away from the one end fixedly connected with baffle of connecting block, one side outer wall welding of baffle has the spring, the spring is kept away from the one end welding of baffle is in on one side inner wall of sorting groove.
In a preferred embodiment, the bottom outer wall of the baffle is fixedly connected with bristles, and one end of the bristles, far away from the baffle, is in contact with the bottom inner wall of the sorting groove.
The invention has the technical effects and advantages that:
1. by arranging the sorting component and the pressurizing component, compared with the prior art, the sample liquid is added into the sorting tank through the liquid inlet pipe by the staff, then the air pump is started by the staff to increase the air pressure in the sorting tank, so that the sample solution can flow rapidly in the sorting groove, and in the process of the rapid flow of the sample solution, the cancer cells and the white blood cells inside the spiral sorting groove are subjected to different inertial forces and dean forces in the spiral sorting groove, so that the cancer cells and the white blood cells are separated at one end of the spiral sorting groove, in the process, the cancer cell solution enters the liquid separating groove, the leucocyte solution enters the waste liquid groove on the other side and is discharged, the spiral sorting groove is used for separating the cancer cells from the white blood cells, so that the structure is simple, meanwhile, no human intervention is needed in the sorting process, and the sorting efficiency is high, so that the use requirements of people are met;
2. through the arrangement of the circulating assembly, compared with the prior art, in order to improve the detection accuracy of the sample solution, workers need to effectively adjust the concentration of the sample solution, after the sample solution is sorted through the sorting assembly, the workers can close the first control valve on one side of the liquid guide pipe, start the pump body, and can suck the cancer cell solution in the liquid separation groove into the sorting groove again through the pump body for sorting for multiple times, so that the concentration of the cancer cell solution can be effectively improved, the loss of the cancer cell solution can be effectively avoided in the process, the concentration of the cancer cell solution can be quickly adjusted, and the use requirements of people are met;
3. through the arrangement of the turbulence component, compared with the prior art, when a worker sorts and concentrates a sample solution, the air pump can be started to inflate and pressurize the interior of the sorting groove, when the air pump inflates and pressurizes the interior of the sorting groove, the air pressure in the interior of the sorting groove can push the baffle to rotate, and when the baffle rotates, the circulation diameter in the interior of the sorting groove can be effectively adjusted, so that the flow resistance of the sample solution in the interior of the sorting groove can be changed, the opening degree of the baffle can be adjusted through the strength of introduced gas, the concentration of the cancer cell solution in the interior of the sorting groove can be further adjusted, the use requirements of people are met, the sample adhered to the bottom of the sorting groove can be easily scraped away in the rotating process of the baffle, and the situation that more sample solution remained in the interior of the sorting groove affects the subsequent detection work of people is avoided;
after the device detects the completion to the sorting of sample solution, when people need clean the device, the baffle that people can start the air pump once more and make rotates, can carry out cleaning work to the bottom inner wall of sorting groove through the rotation of baffle to can improve the clean effect to the device.
Drawings
Fig. 1 is a schematic view of a disassembled structure of the cover plate of the present invention.
Fig. 2 is a schematic view of the overall structure of the present invention.
Fig. 3 is a schematic top view of the first mounting plate according to the present invention.
Fig. 4 is a schematic diagram of a disassembled structure of the first mounting plate of the present invention.
Fig. 5 is a schematic diagram of a second mounting plate of the present invention.
FIG. 6 is a schematic view of the structure of the circulation assembly of the present invention.
FIG. 7 is a schematic view of the overall structure of the sorting tank of the present invention.
Fig. 8 is an enlarged schematic view of the structure at a of the present invention.
The reference signs are: 1. a cover plate; 2. fixing the ear; 3. fixing the screw rod; 4. a connecting pipe; 5. a fixed seat; 6. an air pump; 7. a control panel; 8. a liquid separating tank; 9. a sorting tank; 10. a sealing groove; 11. a housing; 12. sealing the clamping strip; 13. a liquid inlet pipe; 14. a waste liquid tank; 15. a through hole; 16. a first mounting plate; 17. a liquid discharge tank; 18. a shielding plate; 19. a second mounting plate; 20. detecting a plate; 21. a detection tank; 22. a catheter; 23. a first control valve; 24. a liquid pumping pipe; 25. a pump body; 26. a fixed tube; 27. a second control valve; 28. a sewage draining hole; 29. a baffle plate; 30. a spring; 32. a rotating shaft; 33. and (4) connecting the blocks.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The cancer cell sorting and detecting device for the development of the specific binding breast cancer cells as shown in the attached drawings 1-8 comprises a cover plate 1 and a shell 11 arranged below the cover plate 1, wherein the top of the cover plate 1 is provided with a round hole, the circumferential inner wall of the round hole is fixedly connected with a liquid inlet pipe 13, the top of the cover plate 1 is provided with a pressurizing assembly, the inside of the shell 11 is respectively provided with a sorting assembly and a detecting assembly, and the outer wall of one side of the shell 11 is fixedly connected with a control panel 7;
the bottom fixedly connected with circulation subassembly of selecting separately the subassembly, the one end of circulation subassembly communicates with the selection separately the subassembly each other, can separate cancer cell and leucocyte through selecting separately the subassembly, selects separately the subassembly simple structure not only simultaneously, and need not artificial intervention at the selection process, selects separately efficiently the user demand who has satisfied people.
Further, the pressurizing assembly comprises a fixed seat 5 fixedly connected to the outer wall of the top of the cover plate 1, an air pump 6 is fixedly connected to the outer wall of the top of the fixed seat 5, a connecting pipe 4 is connected to the outer wall of one side of the air pump 6 in a plugging mode, one end of the connecting pipe 4 is communicated with the liquid inlet pipe 13, the air pressure inside the sorting assembly can be adjusted by starting the air pump 6, and therefore the sorting efficiency of the sorting assembly on the sample solution can be improved.
Further, the sealing groove 10 has been seted up to the top outer wall of casing 11, and the bottom outer wall fixedly connected with of apron 1 seals card strip 12, and the specification of sealing card strip 12 and the specification looks adaptation of sealing groove 10, the equal fixedly connected with locating component of four side outer walls of casing 11, through mutually supporting between the sealed card strip 12 that sets up and the sealing groove 10, can effectually guarantee holistic leakproofness of device and health nature.
Further, the locating component includes fixed ear 2 of fixed connection at the four side outer walls of apron 1 and casing 11, 2 tops of fixed ear that are located the four side outer walls of casing 11 have seted up threaded hole, 2 tops of fixed ear that are located the four side outer walls of apron 1 have seted up the jack, the circumference inner wall threaded connection of screw hole has clamping screw 3, clamping screw 3's one end passes from the inside of jack, form threaded connection through between clamping screw 3 and the fixed ear 2, can effectually guarantee the compactness of being connected between apron 1 and the casing 11.
Further, the sorting assembly comprises a first mounting plate 16 fixedly connected to the inner wall of the housing 11, a sorting groove 9 is fixedly connected to the outer wall of the top of the first mounting plate 16, the cross section of the sorting groove 9 is spiral, a liquid inlet pipe 13 is positioned right above one end of the sorting groove 9, one end of the sorting groove 9 is fixedly connected with a liquid dividing groove 8 and a waste liquid groove 14, a sewage discharge hole 28 is formed in the inner wall of the bottom of the waste liquid groove 14, a turbulence assembly is arranged inside the sorting groove 9, after the sample solution is added into the sorting groove 9, the cancer cells and the white blood cells inside the sample solution are subjected to different inertial forces and dean forces in the spiral sorting groove 9, so that the cancer cells and the white blood cells are separated at one end of the spiral sorting groove 9, in the process, the cancer cell solution enters the liquid dividing groove 8, the white blood cell solution enters the waste liquid groove 14 on the other side to be discharged, and the cancer cells and the white blood cells are separated by utilizing the spiral sorting groove 9, not only simple structure need not artificial intervention in the sorting process simultaneously, selects separately efficiently and has satisfied people's user demand.
Further, the circulating assembly comprises a liquid guide tube 22 fixedly connected to the outer wall of the bottom of the first mounting plate 16, a through hole 15 is formed in the inner wall of the bottom of the liquid separation groove 8, the liquid guide tube 22 is communicated with the through hole 15, a first control valve 23 is fixedly connected to the outer wall of one side of the liquid guide tube 22, a liquid suction tube 24 is inserted into one side of the liquid suction tube 22, a pump body 25 is inserted into one end, away from the liquid guide tube 22, of the liquid suction tube 24, a fixed tube 26 is inserted into one side of the pump body 25, one end of the fixed tube 26 extends into the separation groove 9, a second control valve 27 is arranged on the outer wall of one side of the fixed tube 26, after the sample solution is separated through the separation assembly, a worker can close the first control valve 23 on one side of the liquid guide tube 22, then the worker starts the pump body 25, the cancer cell solution in the liquid separation groove 8 can be sucked into the separation groove 9 again through the pump body 25 for multiple times of separation, and therefore the concentration of the cancer cell solution can be effectively improved, the loss of the cancer cell solution can be effectively avoided in the process, so that the concentration of the cancer cell solution can be quickly adjusted, and the use requirements of people are met.
Further, the equal fixedly connected with second mounting panel 19 of four side inner walls of casing 11, the top outer wall fixedly connected with shield plate 18 of second mounting panel 19, fluid-discharge tank 17 has been seted up at the top of second mounting panel 19, and fluid-discharge tank 17 is located same vertical face with through-hole 15, and the shield plate 18 that sets up simultaneously can shield ambient signal, can guarantee not disturbed by external electromagnetic wave at sample solution in subsequent testing process, promotes and detects the precision.
Further, the detection assembly comprises a detection plate 20 fixedly connected to the inner walls of the four sides of the housing 11, the detection plate 20 is located below the second mounting plate 19, a detection groove 21 is formed in the top of the detection plate 20, and the cancer cells in the sample solution can be rapidly detected through the detection plate 20.
Further, the turbulence component comprises a connecting block 33 fixedly connected to the inner wall of one side of the separation groove 9, a rotating shaft 32 is rotatably connected to the outer wall of one side of the connecting block 33, a baffle 29 is fixedly connected to one end, far away from the connecting block 33, of the rotating shaft 32, a spring 30 is welded to the outer wall of one side of the baffle 29, one end, far away from the baffle 29, of the spring 30 is welded to the inner wall of one side of the separation groove 9, when a worker separates and concentrates a sample solution, the interior of the separation groove 9 can be inflated and pressurized by starting the air pump 6, when the air pump 6 inflates and pressurizes the interior of the separation groove 9, the air pressure inside the separation groove 9 can push the baffle 29 to rotate, when the baffle 29 rotates, the circulation diameter inside the separation groove 9 can be effectively adjusted, so that the flow resistance of the sample solution inside the separation groove 9 can be changed, therefore, the opening and closing degree of the baffle 29 can be adjusted through the strength of the introduced gas, further can adjust the inside cancer cell solution concentration of sorting tank 9, satisfy people's user demand, and can scrape the sample of adhesion in the sorting tank 9 bottom easily at baffle 29 pivoted in-process, avoid sorting tank 9 inside to remain and have influenced people's subsequent detection work more sample solution.
Further, the bottom outer wall fixedly connected with brush hair of baffle 29, the one end that baffle 29 was kept away from to the brush hair contacts with the bottom inner wall of sorting groove 9, detects the back at the device to the sorting of sample solution, and when people need clean the device, baffle 29 that people can start air pump 6 once more and make rotates, and the same rotation through baffle 29 and brush hair can carry out cleaning work to the bottom inner wall of sorting groove 9 to can improve the clean effect to the device.
The working principle of the invention is as follows: when the operator needs to sort and detect the cancer cells, the operator adds the sample liquid into the sorting tank 9 through the liquid inlet pipe 13, then the operator starts the air pump 6 to increase the air pressure inside the sorting tank 9, so that the sample solution can flow rapidly inside the sorting tank 9, during the rapid flow of the sample solution, the cancer cells and the white blood cells inside the sample solution are subjected to different inertial force and dean force in the spiral sorting tank 9, so that the cancer cells and the white blood cells are separated at one end of the spiral sorting tank 9, during the process, the cancer cell solution enters the liquid separating tank 8, and the white blood cell solution enters the waste liquid tank 14 at the other side to be discharged, the cancer cells and the white blood cells are separated by using the spiral sorting tank 9, the structure is simple, meanwhile, no human intervention is needed during the sorting process, the sorting efficiency is high, and the use requirements of people are met, in order to improve the detection accuracy of the sample solution, the concentration of the sample solution needs to be effectively adjusted by the operator, after the sample solution is sorted by the sorting component, the operator can close the first control valve 23 on one side of the liquid guide tube 22, then the operator starts the pump body 25, the cancer cell solution in the liquid separating tank 8 can be pumped into the sorting tank 9 again through the pump body 25 for sorting for multiple times, so that the concentration of the cancer cell solution can be effectively improved, the loss of the cancer cell solution can be effectively avoided in the process, therefore, the concentration of the cancer cell solution can be quickly adjusted, the use requirements of people are met, when the operator sorts and concentrates the sample solution, the air pump 6 can be started to inflate and pressurize the interior of the sorting tank 9, and when the air pump 6 inflates and pressurizes the interior of the sorting tank 9, the air pressure in the separation groove 9 can push the baffle 29 to rotate, when the baffle 29 rotates, the circulation diameter in the separation groove 9 can be effectively adjusted, so that the flow resistance of the sample solution in the separation groove 9 can be changed, the opening and closing degree of the baffle 29 can be adjusted through the strength of the introduced gas, the concentration of the cancer cell solution in the separation groove 9 can be further adjusted, the use requirements of people are met, the sample adhered to the bottom of the separation groove 9 can be easily scraped away in the rotating process of the baffle 29, the situation that the subsequent detection work of people is influenced by more sample solution remained in the separation groove 9 is avoided, after the separation detection of the sample solution by the device is completed, people can start the air pump 6 again to rotate the baffle 29, and the inner wall at the bottom of the separation groove 9 can be cleaned through the rotation of the baffle 29, thereby enabling an improved cleaning of the device.
The points to be finally explained are: first, in the description of the present application, it should be noted that, unless otherwise specified and limited, the terms "mounted," "connected," and "connected" should be understood broadly, and may be a mechanical connection or an electrical connection, or a communication between two elements, and may be a direct connection, and "upper," "lower," "left," and "right" are only used to indicate a relative positional relationship, and when the absolute position of the object to be described is changed, the relative positional relationship may be changed;
secondly, the method comprises the following steps: in the drawings of the disclosed embodiments of the invention, only the structures related to the disclosed embodiments are referred to, other structures can refer to common designs, and the same embodiment and different embodiments of the invention can be combined with each other without conflict;
and finally: the above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that are within the spirit and principle of the present invention are intended to be included in the scope of the present invention.
Claims (10)
1. A cancer cell sorting and detecting device for specific binding breast cancer cell research comprises a cover plate (1) and a shell (11) arranged below the cover plate (1), and is characterized in that: a round hole is formed in the top of the cover plate (1), a liquid inlet pipe (13) is fixedly connected to the inner wall of the circumference of the round hole, a pressurizing assembly is arranged on the top of the cover plate (1), a sorting assembly and a detection assembly are respectively arranged inside the shell (11), and a control panel (7) is fixedly connected to the outer wall of one side of the shell (11);
the bottom fixedly connected with circulation subassembly of selecting separately the subassembly, the one end of circulation subassembly with select separately the subassembly intercommunication each other.
2. The apparatus for sorting and detecting cancer cells for the research and development of specifically binding breast cancer cells according to claim 1, wherein: the pressurizing assembly comprises a fixed seat (5) fixedly connected to the outer wall of the top of the cover plate (1), an air pump (6) is fixedly connected to the outer wall of the top of the fixed seat (5), a connecting pipe (4) is inserted into the outer wall of one side of the air pump (6), and one end of the connecting pipe (4) is communicated with the liquid inlet pipe (13).
3. The apparatus for sorting and detecting cancer cells for the research and development of specifically binding breast cancer cells according to claim 1, wherein: the sealing groove (10) is formed in the outer wall of the top of the shell (11), the sealing clamping strip (12) is fixedly connected to the outer wall of the bottom of the cover plate (1), the specification of the sealing clamping strip (12) is matched with that of the sealing groove (10), and positioning assemblies are fixedly connected to the outer walls of the four sides of the shell (11).
4. The apparatus for sorting and detecting cancer cells specifically binding to breast cancer cells according to claim 3, wherein: the positioning assembly comprises fixing lugs (2) fixedly connected to the outer walls of the four sides of the cover plate (1) and the shell (11), threaded holes are formed in the tops of the fixing lugs (2) located on the outer walls of the four sides of the shell (11), insertion holes are formed in the tops of the fixing lugs (2) located on the outer walls of the four sides of the cover plate (1), fixing screw rods (3) are connected to the circumferential inner walls of the threaded holes in a threaded mode, and one ends of the fixing screw rods (3) penetrate through the insertion holes.
5. The apparatus for sorting and detecting cancer cells for the research and development of specifically binding breast cancer cells according to claim 1, wherein: sorting subassembly includes fixed connection and is in the first mounting panel (16) of casing (11) inner wall, the top outer wall fixedly connected with of first mounting panel (16) selects separately groove (9), the cross section of selecting separately groove (9) is the screw-tupe, feed liquor pipe (13) are located directly over selecting separately groove (9) one end, the one end fixedly connected with of selecting separately groove (9) divides cistern (8) and waste liquid groove (14), blowoff hole (28) have been seted up to the bottom inner wall of waste liquid groove (14), the inside of selecting separately groove (9) is provided with the vortex subassembly.
6. The apparatus for sorting and detecting cancer cells specifically binding to breast cancer cells according to claim 5, wherein: circulation subassembly includes fixed connection in catheter (22) of first mounting panel (16) bottom outer wall, through-hole (15) have been seted up to the bottom inner wall of branch cistern (8), catheter (22) with through-hole (15) are linked together, one side outer wall fixedly connected with first control valve (23) of catheter (22), it has liquor suction pipe (24) to peg graft in one side of catheter (22), liquor suction pipe (24) are kept away from the one end of catheter (22) is pegged graft and is had pump body (25), one side of pump body (25) is pegged graft and is had fixed pipe (26), the one end of fixed pipe (26) extends to the inside of sorting tank (9), one side outer wall of fixed pipe (26) is provided with second control valve (27).
7. The apparatus for sorting and detecting cancer cells specifically binding to breast cancer cells according to claim 6, wherein: the inner walls of four sides of the shell (11) are fixedly connected with second mounting plates (19), the outer wall of the top of each second mounting plate (19) is fixedly connected with a shielding plate (18), the top of each second mounting plate (19) is provided with a liquid discharge groove (17), and the liquid discharge grooves (17) and the through holes (15) are located on the same vertical surface.
8. The apparatus for sorting and detecting cancer cells specifically binding to breast cancer cells according to claim 7, wherein: the detection assembly comprises a detection plate (20) fixedly connected to the inner walls of the four sides of the shell (11), the detection plate (20) is located below the second mounting plate (19), and a detection groove (21) is formed in the top of the detection plate (20).
9. The apparatus for sorting and detecting cancer cells specifically binding to breast cancer cells according to claim 5, wherein: the vortex subassembly includes fixed connection and is in connecting block (33) of sorting tank (9) one side inner wall, one side outer wall of connecting block (33) rotates and is connected with pivot (32), pivot (32) are kept away from the one end fixedly connected with baffle (29) of connecting block (33), one side outer wall welding of baffle (29) has spring (30), spring (30) are kept away from the one end welding of baffle (29) on the one side inner wall of sorting tank (9).
10. The apparatus for sorting and detecting cancer cells specifically binding to breast cancer cells according to claim 9, wherein: the outer wall of the bottom of the baffle plate (29) is fixedly connected with bristles, and one ends, far away from the baffle plate (29), of the bristles are in contact with the inner wall of the bottom of the sorting groove (9).
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130014322A (en) * | 2011-07-28 | 2013-02-07 | 한국전자통신연구원 | Multiple analysis device and method for analysis of circulating tumor cells using the device |
| CN107699478A (en) * | 2017-09-19 | 2018-02-16 | 朱嗣博 | A kind of circulating tumor cell（CTC）Detection micro flow control chip device |
| CN110653012A (en) * | 2019-10-18 | 2020-01-07 | 遵义市第一人民医院 | Multistage microfluidic chip device for sorting, enriching and detecting circulating tumor cells |
| CN112111385A (en) * | 2020-08-19 | 2020-12-22 | 东南大学 | Cancer cell sorting and detecting device |
-
2021
- 2021-10-15 CN CN202111205055.2A patent/CN113943646B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130014322A (en) * | 2011-07-28 | 2013-02-07 | 한국전자통신연구원 | Multiple analysis device and method for analysis of circulating tumor cells using the device |
| CN107699478A (en) * | 2017-09-19 | 2018-02-16 | 朱嗣博 | A kind of circulating tumor cell（CTC）Detection micro flow control chip device |
| CN110653012A (en) * | 2019-10-18 | 2020-01-07 | 遵义市第一人民医院 | Multistage microfluidic chip device for sorting, enriching and detecting circulating tumor cells |
| CN112111385A (en) * | 2020-08-19 | 2020-12-22 | 东南大学 | Cancer cell sorting and detecting device |
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