CN113943646B - Cancer cell sorting and detecting device for research and development of specifically-combined breast cancer cells - Google Patents
Cancer cell sorting and detecting device for research and development of specifically-combined breast cancer cells Download PDFInfo
- Publication number
- CN113943646B CN113943646B CN202111205055.2A CN202111205055A CN113943646B CN 113943646 B CN113943646 B CN 113943646B CN 202111205055 A CN202111205055 A CN 202111205055A CN 113943646 B CN113943646 B CN 113943646B
- Authority
- CN
- China
- Prior art keywords
- wall
- fixedly connected
- sorting
- groove
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 65
- 201000011510 cancer Diseases 0.000 title claims abstract description 65
- 206010006187 Breast cancer Diseases 0.000 title claims abstract description 12
- 208000026310 Breast neoplasm Diseases 0.000 title claims abstract description 12
- 238000012827 research and development Methods 0.000 title claims abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 73
- 238000000926 separation method Methods 0.000 claims abstract description 61
- 238000001514 detection method Methods 0.000 claims abstract description 32
- 238000007789 sealing Methods 0.000 claims description 10
- 239000002699 waste material Substances 0.000 claims description 10
- 238000003466 welding Methods 0.000 claims description 6
- 230000027455 binding Effects 0.000 claims description 5
- 230000000712 assembly Effects 0.000 claims description 2
- 238000000429 assembly Methods 0.000 claims description 2
- 239000010865 sewage Substances 0.000 claims description 2
- 238000011161 development Methods 0.000 claims 4
- 238000003780 insertion Methods 0.000 claims 2
- 230000037431 insertion Effects 0.000 claims 2
- 230000009870 specific binding Effects 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 17
- 230000008569 process Effects 0.000 abstract description 13
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 49
- 239000012488 sample solution Substances 0.000 description 24
- 239000000243 solution Substances 0.000 description 22
- 239000000523 sample Substances 0.000 description 20
- 210000000265 leukocyte Anatomy 0.000 description 13
- 238000004140 cleaning Methods 0.000 description 5
- 101100460844 Mus musculus Nr2f6 gene Proteins 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- FGRBYDKOBBBPOI-UHFFFAOYSA-N 10,10-dioxo-2-[4-(N-phenylanilino)phenyl]thioxanthen-9-one Chemical compound O=C1c2ccccc2S(=O)(=O)c2ccc(cc12)-c1ccc(cc1)N(c1ccccc1)c1ccccc1 FGRBYDKOBBBPOI-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000005259 peripheral blood Anatomy 0.000 description 3
- 239000011886 peripheral blood Substances 0.000 description 3
- 210000004881 tumor cell Anatomy 0.000 description 3
- RPNUMPOLZDHAAY-UHFFFAOYSA-N Diethylenetriamine Chemical compound NCCNCCN RPNUMPOLZDHAAY-UHFFFAOYSA-N 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 229940041181 antineoplastic drug Drugs 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000036210 malignancy Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000009401 metastasis Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
- C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/10—Waste collection, transportation, transfer or storage, e.g. segregated refuse collecting, electric or hybrid propulsion
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a cancer cell sorting and detecting device for research and development of specifically combined breast cancer cells, and particularly relates to the technical field of cancer cell detection. According to the invention, the cancer cell solution in the liquid separation tank can be pumped into the separation tank again through the pump body for separation for multiple times, so that the concentration of the cancer cell solution can be effectively improved, and the loss of the cancer cell solution can be effectively avoided in the process, so that the concentration of the cancer cell solution can be rapidly regulated.
Description
Technical Field
The invention relates to the technical field of cancer cell detection, in particular to a cancer cell sorting and detecting device for research and development of specifically combined breast cancer cells.
Background
Cancer, also known as malignancy, is a disease caused by an aberration in the control of the mechanisms of cell growth and proliferation. Malignant tumor grows fast and damages normal tissues and organs of human body, and finally causes death of patients. Along with the change of living habits and living environments of people, the current cancer situation is more severe, and the cancer situation becomes the most important problem affecting the public health of the world. Modern medicine has found that in the early stages of cancer recurrence and metastasis, tumor cells fall off from the original tumor focus into the peripheral blood. Tumor cells (also called circulating tumor cells) in peripheral blood are often used for predicting the survival time of cancer patients, and can also be used for guiding cancer diagnosis and prognosis evaluation, thereby providing ideas for developing anticancer drugs. Therefore, the ability to rapidly and efficiently obtain tumor cells from peripheral blood would be of great importance for cancer diagnosis and treatment.
The invention patent of patent application publication No. CN112111385A discloses a cancer cell sorting and detecting device, which completes the sorting and detecting of cancer cells integrally. The cancer cell sorting and detecting device comprises an upper clamp, a sorting unit, a detecting unit and a lower clamp from top to bottom in sequence, wherein the upper clamp is provided with a sample liquid inlet, and the lower clamp is provided with a detecting liquid outlet; the sample liquid inlet is communicated with the inlet of the sorting unit, the outlet of the sorting unit is communicated with the inlet of the detection unit, and the outlet of the detection unit is communicated with the detection liquid outlet. The cancer cell sorting and detecting device can separate cancer cells from the mixed solution of white blood cells and cancer cells, can rapidly and accurately detect the cancer cells, completes the sorting and detecting of the cancer cells integrally, does not need to sort first, and then uses the detecting device for detection, thereby being convenient and quick.
But in actual use, the device needs to cooperate together through a plurality of subassemblies to adjust sample liquid concentration, and in this process, because sample liquid needs to circulate in a plurality of concentrated passageway to can lead to there more sample liquid adhesion at concentrated passageway inner wall in the in-process of circulation, the longer concentrated passageway route is just also bigger the loss of sample liquid, thereby can not effectually adjust the concentration of sample liquid. Therefore, there is a need for a cancer cell sorting and detecting device for developing specifically binding to breast cancer cells to solve the above problems.
Disclosure of Invention
In order to overcome the defects in the prior art, the embodiment of the invention provides a cancer cell sorting and detecting device for research and development of specifically combined breast cancer cells, and the circulating assembly can be used for carrying out circulating liquid separation on sample liquid, so that the concentration of the sample liquid can be regulated, the loss of the sample liquid is avoided, and the problems in the background art are effectively solved.
In order to achieve the above purpose, the present invention provides the following technical solutions: the device comprises a cover plate and a shell arranged below the cover plate, wherein a round hole is formed in the top of the cover plate, a liquid inlet pipe is fixedly connected to the inner wall of the circumference of the round hole, a pressurizing assembly is arranged at the top of the cover plate, a sorting assembly and a detecting assembly are respectively arranged in the shell, and a control panel is fixedly connected to the outer wall of one side of the shell;
the bottom of sorting subassembly fixedly connected with circulation subassembly, circulation subassembly's one end with sorting subassembly intercommunication each other.
In a preferred embodiment, the pressurizing assembly comprises a fixing seat fixedly connected with the outer wall of the top of the cover plate, an air pump is fixedly connected with the outer wall of the top of the fixing seat, a connecting pipe is inserted into the outer wall of one side of the air pump, and one end of the connecting pipe is mutually communicated with the liquid inlet pipe.
In a preferred embodiment, the top outer wall of the shell is provided with a sealing groove, the bottom outer wall of the cover plate is fixedly connected with a sealing clamping strip, the specification of the sealing clamping strip is matched with that of the sealing groove, and the four side outer walls of the shell are fixedly connected with positioning assemblies.
In a preferred embodiment, the positioning assembly comprises fixing lugs fixedly connected with the cover plate and the four-side outer walls of the shell, a threaded hole is formed in the top of each fixing lug located on the four-side outer walls of the shell, a jack is formed in the top of each fixing lug located on the four-side outer walls of the cover plate, a fixing screw is connected with the inner wall of the circumference of each threaded hole in a threaded mode, and one end of each fixing screw penetrates through the jack.
In a preferred embodiment, the separation assembly comprises a first mounting plate fixedly connected with the inner wall of the shell, a separation groove is fixedly connected with the outer wall of the top of the first mounting plate, the cross section of the separation groove is spiral, the liquid inlet pipe is located right above one end of the separation groove, one end of the separation groove is fixedly connected with a liquid separation groove and a waste liquid groove, a sewage draining hole is formed in the inner wall of the bottom of the waste liquid groove, and a turbulence assembly is arranged inside the separation groove.
In a preferred embodiment, the circulation assembly comprises a liquid guide pipe fixedly connected with the outer wall of the bottom of the first mounting plate, a through hole is formed in the inner wall of the bottom of the liquid separation groove, the liquid guide pipe is communicated with the through hole, a first control valve is fixedly connected with the outer wall of one side of the liquid guide pipe, a liquid suction pipe is inserted into one side of the liquid guide pipe, a pump body is inserted into one side of the liquid suction pipe away from the liquid guide pipe, a fixing pipe is inserted into one side of the pump body, one end of the fixing pipe extends to the inside of the separation groove, and a second control valve is arranged on the outer wall of one side of the fixing pipe.
In a preferred embodiment, the four side inner walls of the shell are all fixedly connected with a second mounting plate, the top outer wall of the second mounting plate is fixedly connected with a shielding plate, the top of the second mounting plate is provided with a liquid draining groove, and the liquid draining groove and the through hole are located on the same vertical surface.
In a preferred embodiment, the detection assembly comprises a detection plate fixedly connected to the inner walls of the four sides of the shell, the detection plate is located below the second mounting plate, and a detection groove is formed in the top of the detection plate.
In a preferred embodiment, the vortex subassembly includes fixed connection be in the connecting block of separation tank one side inner wall, one side outer wall of connecting block rotates and is connected with the pivot, the pivot is kept away from one end fixedly connected with baffle of connecting block, one side outer wall welding of baffle has the spring, the spring is kept away from one end welding of baffle is in on one side inner wall of separation tank.
In a preferred embodiment, bristles are fixedly connected to the outer bottom wall of the baffle, and one end of the bristles away from the baffle is in contact with the inner bottom wall of the sorting tank.
The invention has the technical effects and advantages that:
1. compared with the prior art, the device has the advantages that by arranging the sorting assembly and the pressurizing assembly, a worker adds sample liquid into the sorting tank through the liquid inlet pipe, then starts the air pump to increase the air pressure in the sorting tank, so that the sample liquid can flow rapidly in the sorting tank, cancer cells and white blood cells in the sample liquid are subjected to different magnitudes of inertia force and Dien force in the spiral sorting tank in the process of flowing rapidly, the cancer cells and the white blood cells are separated at one end of the spiral sorting tank, the cancer cell solution enters the inside of the liquid separating tank in the process, the white blood cell solution enters the waste liquid tank at the other side and is discharged, and the cancer cells and the white blood cells are separated by utilizing the spiral sorting tank, so that the device is simple in structure, does not need manual intervention in the sorting process, and the sorting efficiency is high, and the use requirements of people are met;
2. compared with the prior art, the circulating assembly is arranged, so that the concentration of the sample solution can be effectively adjusted by workers in order to improve the detection accuracy of the sample solution, after the sample solution is sorted by the sorting assembly, the workers can close the first control valve at one side of the liquid guide tube, then the workers start the pump body, and cancer cell solution in the liquid separation tank can be pumped into the sorting tank again by the pump body for sorting for multiple times, so that the concentration of the cancer cell solution can be effectively improved, the loss of the cancer cell solution can be effectively avoided in the process, and therefore, the concentration of the cancer cell solution can be rapidly adjusted, and the use requirements of people are met;
3. compared with the prior art, through setting up the vortex subassembly, when the staff carries out sorting and concentration work to the sample solution, can aerify pressurization work to the separation tank inside through starting the air pump, when the air pump aerifys pressurization to the separation tank inside, the inside atmospheric pressure of separation tank can promote the baffle and rotate, can effectually adjust the circulation diameter of separation tank inside when the baffle rotates, thereby can change the flow resistance of sample solution at the separation tank inside, consequently, adjust the degree of opening and shutting of baffle through the intensity of letting in gas, further can adjust the inside cancer cell solution concentration of separation tank, the user demand of people has been satisfied, and can scrape the sample of adhesion in separation tank bottom easily at baffle pivoted in-process, it has more sample solution to influence the subsequent detection work of people to avoid the separation tank inside to remain.
After the device is used for sorting and detecting sample solution, when people need to clean the device, people can start the baffle plate which is used by the air pump to rotate again, and cleaning work can be carried out on the inner wall of the bottom of the sorting groove through the rotation of the baffle plate, so that the cleaning effect of the device can be improved.
Drawings
Fig. 1 is a schematic diagram of a cover plate splitting structure of the present invention.
Fig. 2 is a schematic diagram of the overall structure of the present invention.
Fig. 3 is a schematic top view of the first mounting plate of the present invention.
Fig. 4 is a schematic view of a first mounting board split structure according to the present invention.
Fig. 5 is a schematic diagram of a second mounting plate split structure according to the present invention.
FIG. 6 is a schematic view of a circulation assembly structure according to the present invention.
Fig. 7 is a schematic diagram of the overall structure of the sorting tank according to the present invention.
Fig. 8 is an enlarged schematic view of the structure of the present invention at a.
The reference numerals are: 1. a cover plate; 2. a fixed ear; 3. a fixed screw; 4. a connecting pipe; 5. a fixing seat; 6. an air pump; 7. a control panel; 8. a liquid dividing tank; 9. a sorting tank; 10. sealing grooves; 11. a housing; 12. sealing clamping strips; 13. a liquid inlet pipe; 14. a waste liquid tank; 15. a through hole; 16. a first mounting plate; 17. a liquid discharge tank; 18. a shielding plate; 19. a second mounting plate; 20. a detection plate; 21. a detection groove; 22. a catheter; 23. a first control valve; 24. a liquid suction pipe; 25. a pump body; 26. a fixed tube; 27. a second control valve; 28. a blow-down hole; 29. a baffle; 30. a spring; 32. a rotating shaft; 33. and (5) connecting a block.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The cancer cell sorting and detecting device for research and development of specifically combined breast cancer cells as shown in the accompanying drawings 1-8 comprises a cover plate 1 and a shell 11 arranged below the cover plate 1, wherein a round hole is formed in the top of the cover plate 1, a liquid inlet pipe 13 is fixedly connected to the inner wall of the circumference of the round hole, a pressurizing assembly is arranged at the top of the cover plate 1, a sorting assembly and a detecting assembly are respectively arranged in the shell 11, and a control panel 7 is fixedly connected to the outer wall of one side of the shell 11;
the bottom fixedly connected with circulation subassembly of separation subassembly, circulation subassembly's one end and separation subassembly are linked together, can separate cancer cell and leucocyte through separation subassembly, and separation subassembly not only simple structure simultaneously, and need not artificial intervention at the separation process, and separation efficiency is high has satisfied people's user demand.
Further, the pressurization subassembly includes fixing base 5 of fixed connection at apron 1 top outer wall, and the top outer wall fixedly connected with air pump 6 of fixing base 5, and one side outer wall grafting of air pump 6 has connecting pipe 4, and the one end and the feed liquor pipe 13 of connecting pipe 4 communicate each other, can adjust the inside atmospheric pressure of separation subassembly through starting air pump 6 to can improve the separation efficiency of separation subassembly to the sample solution.
Further, seal groove 10 has been seted up to the top outer wall of casing 11, and the bottom outer wall fixedly connected with of apron 1 seals card strip 12, and the specification of sealed card strip 12 and the specification looks adaptation of seal groove 10, and the equal fixedly connected with locating component of four side outer walls of casing 11, through the cooperation between sealed card strip 12 and the seal groove 10 that sets up, can effectually guarantee holistic leakproofness and the sanitation of device.
Further, locating component includes fixed ear 2 of fixed connection at apron 1 and casing 11 four sides outer wall, and threaded hole has been seted up at the fixed ear 2 top that is located casing 11 four sides outer wall, and the jack has been seted up at the fixed ear 2 top that is located apron 1 four sides outer wall, and the circumference inner wall threaded connection of threaded hole has fixing screw 3, and fixing screw 3's one end passes from the inside of jack, forms threaded connection between through fixing screw 3 and the fixed ear 2, can effectually guarantee the compactness of being connected between apron 1 and the casing 11.
Further, select separately the subassembly including fixed connection at the first mounting panel 16 of casing 11 inner wall, the top outer wall fixedly connected with separation tank 9 of first mounting panel 16, separation tank 9's cross section is spiral, feed liquor pipe 13 is located separation tank 9 one end directly over, separation tank 9's one end fixedly connected with divides cistern 8 and waste liquid cistern 14, waste liquid cistern 14's bottom inner wall has seted up blow off hole 28, separation tank 9's inside is provided with vortex subassembly, after sample solution adds to separation tank 9 inside, sample solution inside cancer cell and white blood cell receive the inertial force and the dien's of equidimension in spiral separation tank 9, thereby make cancer cell and white blood cell separated in spiral separation tank 9's one end, cancer cell solution gets into separation tank 8 inside in this process, and white blood cell solution gets into the waste liquid cistern 14 of opposite side and discharges, through utilizing spiral separation tank 9 to separate cancer cell and white blood cell, not only simple structure, need not artificial intervention simultaneously at separation process, separation efficiency is high has satisfied people's user demand.
Further, circulation subassembly includes catheter 22 of fixed connection at first mounting panel 16 bottom outer wall, through-hole 15 has been seted up to the bottom inner wall of branch cistern 8, catheter 22 is linked together with through-hole 15, one side outer wall fixedly connected with first control valve 23 of catheter 22, one side grafting of catheter 22 has the liquid-extracting tube 24, the one end that liquid-extracting tube 24 kept away from catheter 22 has pegged graft the pump body 25, one side grafting of pump body 25 has fixed pipe 26, the one end of fixed pipe 26 extends to the inside of sorting tank 9, one side outer wall of fixed pipe 26 is provided with second control valve 27, after the sample solution is selected separately through the separation subassembly, the staff can close the first control valve 23 of catheter 22 one side, then the staff starts the pump body 25, can draw in the inside cancer cell solution of branch cistern 8 again and select separately the inside of 9 a lot of carrying out the separation through pump body 25, thereby can effectually improve the concentration of cancer cell solution, the loss of cancer cell solution can effectually be avoided in this process, consequently, can be quick adjust the concentration of cancer cell solution, people's user demand has been satisfied.
Further, the four side inner walls of the shell 11 are fixedly connected with the second mounting plate 19, the top outer wall of the second mounting plate 19 is fixedly connected with the shielding plate 18, the top of the second mounting plate 19 is provided with the liquid discharge groove 17, the liquid discharge groove 17 and the through hole 15 are located on the same vertical surface, and meanwhile, the shielding plate 18 can shield external signals, so that the sample solution can be prevented from being interfered by external electromagnetic waves in the subsequent detection process, and the detection precision is improved.
Further, the detection assembly comprises a detection plate 20 fixedly connected to the inner walls of the four sides of the shell 11, the detection plate 20 is located below the second mounting plate 19, a detection groove 21 is formed in the top of the detection plate 20, and cancer cells in the sample solution can be rapidly detected through the detection plate 20.
Further, vortex subassembly includes the connecting block 33 of fixed connection at sorting tank 9 one side inner wall, one side outer wall rotation of connecting block 33 is connected with pivot 32, one end fixedly connected with baffle 29 of connecting block 33 is kept away from to pivot 32, one side outer wall welding of baffle 29 has spring 30, one end welding that baffle 29 was kept away from to spring 30 is on sorting tank 9 one side inner wall, when the staff carries out sorting and concentration work to the sample solution, can aerify pressurization work to sorting tank 9 inside through starting air pump 6, when air pump 6 carries out the pressurization to sorting tank 9 inside, the inside atmospheric pressure of sorting tank 9 can promote baffle 29 rotation, can effectually adjust the inside circulation diameter of sorting tank 9 when baffle 29 rotates, thereby can change the flow resistance of sample solution in sorting tank 9 inside, therefore the degree of opening and shutting of baffle 29 is adjusted through the intensity of letting in gas, further can adjust the inside cancer cell solution concentration of sorting tank 9, people's user demand has been satisfied, and in the in-process that baffle 29 pivoted can be with the sample of adhesion in sorting tank 9 bottom can scrape easily, the inside the sorting tank 9 has left more sample solution of influence the work of detecting later.
Further, the bottom outer wall fixedly connected with brush hair of baffle 29, the one end that baffle 29 was kept away from to the brush hair contacts with the bottom inner wall of sorting tank 9, and when the device is to the sorting detection of sample solution accomplish the back, people need to clean the device, and people can start the baffle 29 rotation that air pump 6 made again, can carry out cleaning work to the bottom inner wall of sorting tank 9 through the rotation of baffle 29 and brush hair together to can improve the cleaning performance to the device.
The working principle of the invention is as follows: when the staff needs to sort and detect cancer cells, the staff adds sample liquid into the sorting tank 9 through the liquid inlet pipe 13, then the staff starts the air pump 6 to increase the air pressure in the sorting tank 9, so that the sample liquid can flow rapidly in the sorting tank 9, in the process of the sample liquid flowing rapidly, cancer cells and white blood cells in the sample liquid are subjected to different magnitudes of inertia force and dean force in the spiral sorting tank 9, so that the cancer cells and the white blood cells are separated at one end of the spiral sorting tank 9, in the process, the cancer cell solution enters the inside of the liquid separating tank 8, and the white blood cell solution enters the waste liquid tank 14 at the other side and is discharged, by utilizing the spiral sorting tank 9 to separate the cancer cells and the white blood cells, the structure is simple, no manual intervention is needed in the sorting process, the sorting efficiency is high, the use requirements of people are met, in order to improve the detection accuracy of the sample solution, the worker needs to effectively adjust the concentration of the sample solution, after the sample solution is sorted by the sorting assembly, the worker can close the first control valve 23 at one side of the catheter 22, then the worker starts the pump body 25, the cancer cell solution in the liquid separation tank 8 can be pumped into the sorting tank 9 again by the pump body 25 for sorting for a plurality of times, so that the concentration of the cancer cell solution can be effectively improved, the loss of the cancer cell solution can be effectively avoided in the process, the concentration of the cancer cell solution can be quickly adjusted, the use requirement of the worker is met, when the worker sorts and concentrates the sample solution, the air pump 6 can be started to perform inflation and pressurization work in the sorting tank 9, and when the air pump 6 performs inflation and pressurization in the sorting tank 9, the inside atmospheric pressure of separation groove 9 can promote baffle 29 rotation, can effectually adjust the inside circulation diameter of separation groove 9 when baffle 29 rotates, thereby can change the flow resistance of sample solution at separation groove 9 inside, consequently adjust baffle 29's opening and shutting degree through the intensity of letting in gas, further can adjust separation groove 9 inside cancer cell solution concentration, the user demand of people has been satisfied, and can scrape the sample of adhesion in separation groove 9 bottom easily at baffle 29 pivoted in-process, avoid separation groove 9 inside to remain there being more sample solution to influence people subsequent detection work, after the separation detection of device to sample solution is accomplished, people need to clean the device when, people can start the baffle 29 rotation that air pump 6 made again, can clean the bottom inner wall of separation groove 9 through baffle 29's rotation, thereby can improve the cleaning effect to the device.
The last points to be described are: first, in the description of the present application, it should be noted that, unless otherwise specified and defined, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be mechanical or electrical, or may be a direct connection between two elements, and "upper," "lower," "left," "right," etc. are merely used to indicate relative positional relationships, which may be changed when the absolute position of the object being described is changed;
secondly: in the drawings of the disclosed embodiments, only the structures related to the embodiments of the present disclosure are referred to, and other structures can refer to the common design, so that the same embodiment and different embodiments of the present disclosure can be combined with each other under the condition of no conflict;
finally: the foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (5)
1. The utility model provides a cancer cell sorting and detecting device for research and development of specific binding breast cancer cells, includes apron (1) and set up in casing (11) of apron (1) below, its characterized in that: the top of the cover plate (1) is provided with a round hole, the circumferential inner wall of the round hole is fixedly connected with a liquid inlet pipe (13), the top of the cover plate (1) is provided with a pressurizing assembly, the inside of the shell (11) is respectively provided with a sorting assembly and a detecting assembly, and the outer wall of one side of the shell (11) is fixedly connected with a control panel (7);
the bottom of the sorting assembly is fixedly connected with a circulating assembly, and one end of the circulating assembly is communicated with the sorting assembly;
the pressurizing assembly comprises a fixed seat (5) fixedly connected to the outer wall of the top of the cover plate (1), an air pump (6) is fixedly connected to the outer wall of the top of the fixed seat (5), a connecting pipe (4) is inserted into the outer wall of one side of the air pump (6), and one end of the connecting pipe (4) is communicated with the liquid inlet pipe (13);
a sealing groove (10) is formed in the outer wall of the top of the shell (11), a sealing clamping strip (12) is fixedly connected to the outer wall of the bottom of the cover plate (1), the specification of the sealing clamping strip (12) is matched with that of the sealing groove (10), and positioning assemblies are fixedly connected to the four side outer walls of the shell (11);
the positioning assembly comprises fixing lugs (2) fixedly connected to the cover plate (1) and the four-side outer walls of the shell (11), threaded holes are formed in the tops of the fixing lugs (2) positioned on the four-side outer walls of the shell (11), insertion holes are formed in the tops of the fixing lugs (2) positioned on the four-side outer walls of the cover plate (1), fixing screws (3) are connected with the circumferential inner walls of the threaded holes in a threaded mode, and one ends of the fixing screws (3) penetrate through the insertion holes;
the sorting assembly comprises a first mounting plate (16) fixedly connected to the inner wall of the shell (11), a sorting groove (9) is fixedly connected to the outer wall of the top of the first mounting plate (16), the cross section of the sorting groove (9) is spiral, the liquid inlet pipe (13) is positioned right above one end of the sorting groove (9), one end of the sorting groove (9) is fixedly connected with a liquid separating groove (8) and a waste liquid groove (14), a sewage draining hole (28) is formed in the inner wall of the bottom of the waste liquid groove (14), and a turbulence assembly is arranged in the sorting groove (9);
the circulating assembly comprises a liquid guide tube (22) fixedly connected with the outer wall of the bottom of the first mounting plate (16), a through hole (15) is formed in the inner wall of the bottom of the liquid separation groove (8), the liquid guide tube (22) is communicated with the through hole (15), a first control valve (23) is fixedly connected with the outer wall of one side of the liquid guide tube (22), a liquid suction tube (24) is inserted and connected to one side of the liquid guide tube (22), a pump body (25) is inserted and connected to one side of the liquid suction tube (24) away from one end of the liquid guide tube (22), a fixing tube (26) is inserted and connected to one side of the pump body (25), one end of the fixing tube (26) extends to the inside of the separation groove (9), and a second control valve (27) is arranged on the outer wall of one side of the fixing tube (26).
2. The cancer cell sorting and detecting device for development of specifically-binding breast cancer cells according to claim 1, wherein: the novel anti-theft device is characterized in that second mounting plates (19) are fixedly connected to four side inner walls of the shell (11), shielding plates (18) are fixedly connected to the top outer walls of the second mounting plates (19), liquid discharge grooves (17) are formed in the tops of the second mounting plates (19), and the liquid discharge grooves (17) and the through holes (15) are located on the same vertical surface.
3. The cancer cell sorting and detecting device for development of specifically-binding breast cancer cells according to claim 1, wherein: the detection assembly comprises a detection plate (20) fixedly connected to the inner walls of the four sides of the shell (11), the detection plate (20) is located below the second mounting plate (19), and a detection groove (21) is formed in the top of the detection plate (20).
4. The cancer cell sorting and detecting device for development of specifically-binding breast cancer cells according to claim 1, wherein: the vortex subassembly includes fixed connection be in connecting block (33) of separation tank (9) one side inner wall, one side outer wall rotation of connecting block (33) is connected with pivot (32), pivot (32) are kept away from one end fixedly connected with baffle (29) of connecting block (33), one side outer wall welding of baffle (29) has spring (30), one end welding that spring (30) kept away from baffle (29) is in on one side inner wall of separation tank (9).
5. The device for sorting and detecting cancer cells for development of specifically-binding breast cancer cells according to claim 4, wherein: the bottom outer wall of baffle (29) fixedly connected with brush hair, the brush hair keep away from the one end of baffle (29) with the bottom inner wall of separation groove (9) contacts.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111205055.2A CN113943646B (en) | 2021-10-15 | 2021-10-15 | Cancer cell sorting and detecting device for research and development of specifically-combined breast cancer cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111205055.2A CN113943646B (en) | 2021-10-15 | 2021-10-15 | Cancer cell sorting and detecting device for research and development of specifically-combined breast cancer cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113943646A CN113943646A (en) | 2022-01-18 |
| CN113943646B true CN113943646B (en) | 2023-12-19 |
Family
ID=79330883
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111205055.2A Active CN113943646B (en) | 2021-10-15 | 2021-10-15 | Cancer cell sorting and detecting device for research and development of specifically-combined breast cancer cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113943646B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130014322A (en) * | 2011-07-28 | 2013-02-07 | 한국전자통신연구원 | Multiple analysis device and method for analysis of circulating tumor cells using the device |
| CN107699478A (en) * | 2017-09-19 | 2018-02-16 | 朱嗣博 | A kind of circulating tumor cell（CTC）Detection micro flow control chip device |
| CN110653012A (en) * | 2019-10-18 | 2020-01-07 | 遵义市第一人民医院 | Multistage microfluidic chip device for sorting, enriching and detecting circulating tumor cells |
| CN112111385A (en) * | 2020-08-19 | 2020-12-22 | 东南大学 | Cancer cell sorting and detecting device |
-
2021
- 2021-10-15 CN CN202111205055.2A patent/CN113943646B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20130014322A (en) * | 2011-07-28 | 2013-02-07 | 한국전자통신연구원 | Multiple analysis device and method for analysis of circulating tumor cells using the device |
| CN107699478A (en) * | 2017-09-19 | 2018-02-16 | 朱嗣博 | A kind of circulating tumor cell（CTC）Detection micro flow control chip device |
| CN110653012A (en) * | 2019-10-18 | 2020-01-07 | 遵义市第一人民医院 | Multistage microfluidic chip device for sorting, enriching and detecting circulating tumor cells |
| CN112111385A (en) * | 2020-08-19 | 2020-12-22 | 东南大学 | Cancer cell sorting and detecting device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113943646A (en) | 2022-01-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20240173716A1 (en) | Microfluidic chip, and automatic separation and detection system and method for circulating tumor cell | |
| CN205027766U (en) | Arena analysis appearance | |
| CN113943646B (en) | Cancer cell sorting and detecting device for research and development of specifically-combined breast cancer cells | |
| US20210170409A1 (en) | Microfluidic chip for circulating tumor cell separation, circulating tumor cell separation method and counting method | |
| CN106442323A (en) | New clinical laboratory blood analysis instrument | |
| CN107988042B (en) | Circulating tumor cell separation device and detection method thereof | |
| CN109234152A (en) | A kind of circulating tumor cell separator | |
| CN109374913B (en) | Liquid path system device and control method | |
| CN204462005U (en) | A kind of water quality heavy metal on-line sample detection system | |
| CN209485830U (en) | The device of hospital and the home-use extracellular vesica of enrichment urine that can make | |
| CN208537233U (en) | A kind of cluster sampling cabinet for sewage treatment | |
| CN208520615U (en) | A kind of parasitism fecula stool detection sampler | |
| CN110283714A (en) | Separator is detected based on Terahertz and micro-fluidic circulating tumor cell | |
| CN116068156A (en) | Device for detecting chronic diseases based on body fluid | |
| CN212845349U (en) | Novel water quality testing analysis is special device | |
| CN115112463A (en) | Micro blood filtering device and application thereof in blood nutrient element detection | |
| CN210103484U (en) | Detection mechanism for sewage treatment | |
| CN108414511A (en) | A kind of tumour urine detector and method | |
| CN211633391U (en) | Colonoscope specimen collecting device | |
| CN220304902U (en) | Automatic blood sample adding device and cell enrichment and dyeing integrated equipment | |
| CN220323328U (en) | Western blot antibody incubation and membrane washing device | |
| CN213022435U (en) | A liquid medicine sampling device for pharmaceutical analysis | |
| CN219996893U (en) | Kit for rapidly detecting cytoheme in urine | |
| CN211652464U (en) | Lung cancer circulating tumor cell detection device | |
| CN214621937U (en) | Device for separating biological fluid from tissue based on vacuum pumping |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |