CN113941454A - Centrifugal device and centrifugal method for collecting Cyr61 target conditioned medium - Google Patents
Centrifugal device and centrifugal method for collecting Cyr61 target conditioned medium Download PDFInfo
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- CN113941454A CN113941454A CN202111151536.XA CN202111151536A CN113941454A CN 113941454 A CN113941454 A CN 113941454A CN 202111151536 A CN202111151536 A CN 202111151536A CN 113941454 A CN113941454 A CN 113941454A
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- cyr61
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- conditioned medium
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
- B04B2005/0478—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation with filters in the separation chamber
Abstract
The invention discloses a centrifugal device and a centrifugal method for collecting a Cyr61 target conditioned medium, wherein the centrifugal device for collecting the Cyr61 target conditioned medium comprises a supporting component, a centrifugal component, a filtering component and a liquid taking component, the supporting component comprises a base, a shell and a supporting frame, the centrifugal component comprises a driving motor, a connecting shaft, a supporting arm and a fixing piece, the filtering component comprises an electric push rod, a connecting frame and a plurality of filtering pieces, and the liquid taking component comprises a suction pump, a main pipe, a plurality of branch pipes and a plurality of suction heads. According to the invention, the culture solution is simply and conveniently separated, and meanwhile, after the separation is finished, the filtration is carried out again and the supernatant is sucked, so that a good separation effect is achieved, and the quality of the supernatant is ensured.
Description
Technical Field
The invention relates to the technical field of centrifugation, in particular to a centrifugal device and a centrifugal method for collecting a Cyr61 target conditioned medium.
Background
The prognosis of the liver metastasis of the pancreatic cancer is poor, so far, an effective treatment means is lacked, and the search for a new treatment target point has important clinical value. Project group early-stage projects prove that Cyr61 is regulated to regulate EMT to inhibit pancreatic cancer liver metastasis when clearing pancreas stasis, and in subsequent researches, people find that the pancreas stasis clearing formula can inhibit tumor-associated macrophage (TAM) infiltration and IL-6 secretion. Therefore, the hypothesis is proposed: the effect of regulating EMT to inhibit pancreatic cancer liver metastasis by Cyr61 in clearing pancreas accumulation is dependent on the participation of TAM in a tumor microenvironment, and Cyr61 can promote the growth and metastasis of pancreatic cancer cells by regulating and controlling a tumor-related macrophage-pancreatic cancer cell interaction feedback loop. In order to verify the hypothesis, the action mechanism of Cyr61 for regulating and controlling tumor-associated macrophage-pancreatic cancer cell interaction and promoting the growth and metastasis of pancreatic cancer cells is determined by means of transfection of liposome vectors, a pancreatic cancer cell and macrophage co-culture system and the like.
In the process of verification, in vitro and in vivo experiments that Cyr61 regulates and controls interaction between tumor-associated macrophages and pancreatic cancer cells to promote growth and metastasis of pancreatic cancer cells are needed. The experiment specifically comprises the steps of pancreatic cancer specimen collection and gene expression analysis, construction of Cyr61 eukaryotic expression plasmid, establishment of Cyr61 stable transfection cell line, collection of Cyr61 conditioned medium and the like, and the specific steps of establishing Cyr61 stable transfection cell line and collection of Cyr61 conditioned medium are as follows: transfecting pcDNA3.1-Cyr61 plasmid into pancreatic cancer cells (PANC-1 and BxPC-3), after 24 hours of transfection, transferring into a culture medium containing G418 antibiotic, culturing for 2-3 weeks to obtain G418 resistant pancreatic cancer cell clone, and carrying out enzyme digestion and mixing on the cell clone to obtain a Cyr61 stable transfected cell line; culturing the transfected cells in a serum-free culture medium for 24 hours, then collecting the culture solution, centrifuging for 5min at 1000 rpm, removing cell debris, subpackaging the supernatant which is the Cyr61 conditioned medium, and freezing for storage.
Centrifugation plays important effect to follow-up experiment as collecting an important ring in the Cyr61 conditioned medium, however, current centrifugal device is in the use, and the supernatant is discharged inconveniently, and after the centrifugation finishes, lacks further filtration to the supernatant, leads to the supernatant quality to worsen, influences follow-up experiment.
Disclosure of Invention
Technical problem to be solved
The invention can solve the problems that the prior centrifugal device is inconvenient in discharging the supernatant, and the quality of the supernatant is deteriorated and subsequent experiments are influenced because the supernatant is lack of further filtration after the centrifugation is finished.
(II) technical scheme
In order to achieve the above object, the present invention provides a centrifuge device for collecting Cyr61 target conditioned medium, comprising a support component, a centrifuge component, a filter component and a liquid-taking component.
The supporting component comprises a base, a shell and a supporting frame, the shell is installed on the upper portion of the base, the interior of the shell is arranged in a hollow mode, and the supporting frame is installed on the upper portion of the shell.
The centrifugal component comprises a driving motor, a connecting shaft, a supporting arm and a fixing piece, wherein the driving motor is installed on the upper portion of the base, the connecting shaft is installed at the output end of the driving motor, the connecting shaft extends into the shell, the supporting arm is installed on the side portion of the connecting shaft, and the fixing piece is installed on the upper portion of the supporting arm.
The filter assembly comprises an electric push rod, a connecting frame and a plurality of filter pieces, the electric push rod is installed on the upper portion of the center of the support frame, the connecting frame is installed at the output end of the electric push rod, the filter pieces are installed on the lower portion of the connecting frame respectively, and the lower portions of the filter pieces are opposite to the upper portion of the fixing piece.
Get liquid subassembly and include suction pump, female pipe, a plurality of branch pipe and a plurality of suction head, the suction pump install in the upper portion of support frame, female union coupling in the feed liquor end of suction pump, it is a plurality of the branch pipe connect respectively in the feed liquor end of female pipe, it is a plurality of the suction head is installed respectively in a plurality of the feed liquor end of branch pipe, it is a plurality of branch pipe and a plurality of the suction head install respectively in the lateral part of link, it is a plurality of the suction head respectively with a plurality of it is adjacent to filter the piece.
As a preferred technical scheme of the invention, the middle part of the base is arranged in a hollow way, and the outer contour of the whole section of the base is arranged in an isosceles trapezoid.
As a preferable technical scheme of the invention, the middle part of the shell is provided with a through hole matched with the connecting shaft, and the inner wall of the shell is in clearance fit with the side part of the supporting arm.
As a preferred technical solution of the present invention, the fixing member includes a supporting plate and a plurality of position limiting rings, the supporting plate is mounted on the upper portion of the supporting arm, the upper portion of the supporting plate is provided with a plurality of grooves, and the plurality of position limiting rings are respectively mounted on the inner sides of the supporting plate adjacent to the grooves.
As a preferable technical solution of the present invention, the center of the support plate and the center of the connecting shaft are coaxially disposed, and lower portions of the plurality of grooves are respectively opposite to lower portions of the plurality of filter elements.
As a preferred technical scheme of the present invention, the filter member includes a connection sleeve, a primary filter screen and a high efficiency filter screen, the connection sleeve is installed at a lower portion of the connection frame, the primary filter screen and the high efficiency filter screen are respectively installed at inner sides of the connection sleeve, and the high efficiency filter screen is located at an upper portion of the primary filter screen.
As a preferable technical scheme of the invention, the outer side of the connecting sleeve is glued with a sealing ring, the lower part of the sealing ring is provided with a guide part, and the section of the guide part is arranged in a conical shape.
As a preferable technical solution of the present invention, the main pipe is provided as a hose.
As a preferable technical scheme of the invention, a pipe clamp matched with the branch pipe is arranged on the side part of the connecting frame, and a flexible pad is arranged on the inner side of the pipe clamp.
In addition, the invention also provides a centrifugation method of the centrifugation device for collecting the Cyr61 target conditioned medium, which comprises the following steps:
s1, fixing the test tube: placing the culture solution to be centrifuged into a plurality of test tubes, wherein the height of the culture solution is not higher than two thirds of the total height of the test tubes, and then fixing the test tubes on the upper parts of the fixing parts;
s2, culture solution centrifugation: running a driving motor, wherein the driving motor runs to drive a connecting shaft, a supporting arm and a fixing piece to rotate so as to rotate a plurality of test tubes, broken cells are gathered on the side walls of the test tubes under the action of centrifugal force, and then the test tubes are kept stand for 1h-3h so as to gather the broken cells at the bottoms of the test tubes under the action of gravity;
s3, filtering the culture solution: the electric push rod is operated to drive the connecting frame to move downwards, so that the plurality of filtering pieces are inserted into the plurality of test tubes, the culture solution is filtered again in the process of descending the filtering pieces, and the broken cells which do not fall down are filtered to the bottoms of the test tubes;
s4, extracting the supernatant, wherein in the process that the connecting frame moves downwards, the suction head extends into the test tube, then the suction pump is operated, the supernatant on the upper part of the test tube is sucked by the operation of the suction pump, and the supernatant is discharged into an external storage container through the suction head, the branch tube, the mother tube and the suction pump in sequence.
(III) advantageous effects
1. The support component of the centrifugal device for collecting the Cyr61 target conditioned medium comprises a base, a shell and a support frame, the support component is prevented from being arranged on an experiment table to be used through the base, the integral support is convenient, the shell provides a centrifugal space, and the support frame is convenient for supporting a filtering component and a liquid taking component;
2. the centrifugal device for collecting the Cyr61 target conditioned medium comprises a centrifugal assembly, a centrifugal component and a centrifugal device, wherein the centrifugal assembly comprises a driving motor, a connecting shaft, a supporting arm and a fixing piece;
3. according to the centrifugal device for collecting the Cyr61 target conditioned medium, the filtering component comprises the electric push rod, the connecting frame and the plurality of filtering pieces, the connecting frame is driven to ascend and descend by the operation of the electric push rod, so that the filtering pieces ascend and descend, and the filtering pieces can conveniently extend into a test tube;
4, the centrifugal device for collecting the Cyr61 target conditioned medium provided by the invention comprises a liquid taking assembly, a liquid taking assembly and a centrifugal device, wherein the liquid taking assembly comprises a suction pump, a main pipe, a plurality of branch pipes and a plurality of suction heads, the suction heads extend into the test tube in the process of moving the connecting frame downwards, then the suction pump is operated, the suction pump is operated to suck the supernatant on the upper part of the test tube, and the supernatant is discharged into an external storage container through the suction heads, the branch pipes, the main pipe and the suction pump in sequence.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a front cross-sectional schematic view of the present invention;
FIG. 2 is a schematic front view of a portion of the support tray of the present invention;
FIG. 3 is a schematic top view of a support plate portion configuration of the present invention;
FIG. 4 is an enlarged schematic view at A of the present invention;
fig. 5 is a schematic view of a part of the pipe clamp structure of the present invention.
In the figure: 100. a support assembly; 110. a base; 120. a housing; 121. a through hole; 130. a support frame; 200. a centrifuge assembly; 210. a drive motor; 220. a connecting shaft; 230. a support arm; 240. a fixing member; 241. a support disc; 242. a limiting ring; 243. a groove; 300. a filter assembly; 310. an electric push rod; 320. a connecting frame; 321. a pipe clamp; 322. a flexible pad; 330. a filter member; 331. connecting sleeves; 332. a primary filter screen; 333. a high-efficiency filter screen; 334. a seal ring; 335. a guide portion; 400. a liquid taking assembly; 410. a suction pump; 420. a main pipe; 430. a branch pipe; 440. a suction head.
The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it is to be understood that the terms "longitudinal", "upper", "lower", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Example 1
As shown in fig. 1 to 5, a centrifuge device for collecting Cyr61 target conditioned medium comprises a support assembly 100, a centrifuge assembly 200, a filter assembly 300 and a liquid extraction assembly 400.
The supporting assembly 100 comprises a base 110, a housing 120 and a supporting frame 130, wherein the housing 120 is mounted on the upper portion of the base 110, and when the supporting assembly is specifically arranged, in order to achieve a better supporting effect on the whole body, the middle portion of the base 110 is arranged in a hollow manner, and the outer contour of the whole section of the base 110 is arranged in an isosceles trapezoid shape; the housing 120 is hollow, the upper portion of the housing 120 is open, and the support 130 is mounted on the upper portion of the housing 120 by bolts.
The centrifugal assembly 200 includes a driving motor 210, a connecting shaft 220, a supporting arm 230 and a fixing member 240, the driving motor 210 is mounted on the upper portion of the base 110, the connecting shaft 220 is mounted on the output end of the driving motor 210, the connecting shaft 220 extends into the interior of the housing 120, the supporting arm 230 is mounted on the side portion of the connecting shaft 220, and the fixing member 240 is mounted on the upper portion of the supporting arm 230.
In particular, in order to enable the connecting shaft 220 to effectively rotate, the middle of the housing 120 is provided with a through hole 121 matched with the connecting shaft 220, and in order to ensure that the supporting arm 230 effectively rotates, the inner wall of the housing 120 is in clearance fit with the side portion of the supporting arm 230.
In this embodiment, the fixing member 240 includes a supporting plate 241 and a plurality of position-limiting rings 242, the supporting plate 241 is installed on the upper portion of the supporting arm 230, the upper portion of the supporting plate 241 is provided with a plurality of grooves 243, the grooves 243 are arranged in an annular array relative to the center of the supporting plate 241, the plurality of position-limiting rings 242 are respectively installed on the inner sides of the supporting plate 241 adjacent to the grooves 243, and the position-limiting rings 242 are arranged as elastic rubber rings, so that the test tube can be better fixed; when specifically setting up, the center of supporting disk 241 and the coaxial setting in center of connecting axle 220, during the specific use, insert the inside of recess 243 respectively with a plurality of test tubes to carry out spacingly and fixed to the test tube through spacing ring 242, reach the good fixed to the test tube.
The filtering assembly 300 includes an electric pushing rod 310, a connecting frame 320 and a plurality of filtering pieces 330, the electric pushing rod 310 is mounted on the upper portion of the center of the supporting frame 130 through bolts, the connecting frame 320 is mounted on the output end of the electric pushing rod 310, the plurality of filtering pieces 330 are respectively mounted on the lower portion of the connecting frame 320, the lower portions of the plurality of filtering pieces 330 are opposite to the upper portion of the fixing piece 240, and when the filtering assembly is specifically set, the lower portions of the plurality of grooves 243 are respectively opposite to the lower portions of the plurality of filtering pieces 330.
In this embodiment, the filter member 330 includes a connection sleeve 331, a primary filter screen 332 and a high efficiency filter screen 333, the connection sleeve 331 is installed at the lower part of the connection frame 320, the primary filter screen 332 and the high efficiency filter screen 333 are respectively installed at the inner side of the connection sleeve 331, and the high efficiency filter screen 333 is located at the upper part of the primary filter screen 332; when the test tube is specifically arranged, the sealing ring 334 is glued to the outer side of the connecting sleeve 331, the guide part 335 is arranged at the lower part of the sealing ring 334, the section of the guide part 335 is arranged in a conical shape, the guide part 335 is used for guiding, the sealing ring 334 can be conveniently inserted into the test tube, and the guide part 335 and the sealing ring 334 are integrally arranged; during the specific use, the adapter sleeve follows the lift and the lift of link 320, inserts the inside of test tube to utilize the inner wall contact of sealing ring 334 with the test tube, seal, utilize the cooperation of primary filter screen 332 and high-efficient filter screen 333, realize the filtration to the culture solution.
The liquid taking assembly 400 comprises a suction pump 410, a main pipe 420, a plurality of branch pipes 430 and a plurality of suction heads 440, wherein the suction pump 410 is installed at the upper part of the support frame 130, the main pipe 420 is connected to the liquid inlet end of the suction pump 410, the main pipe 420 is provided as a hose, the plurality of branch pipes 430 are respectively connected to the liquid inlet end of the main pipe 420, the plurality of suction heads 440 are respectively installed at the liquid inlet ends of the plurality of branch pipes 430, the plurality of branch pipes 430 and the plurality of suction heads 440 are respectively installed at the side parts of the connecting frame 320, in the specific arrangement, the side parts of the connecting frame 320 are provided with pipe clamps 321 matched with the branch pipes 430, and the inner sides of the pipe clamps 321 are provided with flexible pads 322; a plurality of suction heads 440 are respectively adjacent to the plurality of filters 330.
In addition, the invention also provides a centrifugation method of the centrifugation device for collecting the Cyr61 target conditioned medium, which comprises the following steps:
s1, fixing the test tube: putting the culture solution to be centrifuged into a plurality of test tubes, wherein the height of the culture solution is not higher than two thirds of the total height of the test tubes, and then fixing the test tubes on the upper parts of the fixing pieces 240;
s2, culture solution centrifugation: the driving motor 210 is operated, the driving motor 210 drives the connecting shaft 220, the supporting arm 230 and the fixing piece 240 to rotate, so that the test tubes rotate, the broken cells are gathered on the side walls of the test tubes under the action of centrifugal force, and then the test tubes are kept stand for 1h-3h, so that the broken cells are gathered at the bottoms of the test tubes under the action of gravity;
s3, filtering the culture solution: when the electric push rod 310 is operated, the electric push rod 310 drives the connecting frame 320 to move downwards, so that the plurality of filtering pieces 330 are inserted into the plurality of test tubes, the culture solution is filtered again in the process of descending the filtering pieces 330, and the broken cells which do not fall down are filtered to the bottoms of the test tubes;
and S4, extracting the supernatant, wherein in the process that the connecting frame 320 moves downwards, the suction head 440 extends into the test tube, then the suction pump 410 is operated, the operation of the suction pump 410 sucks the supernatant on the upper part of the test tube, and the supernatant passes through the suction head 440, the branch tube 430, the main tube 420 and the suction pump 410 in sequence and is discharged into an external storage container.
In conclusion, the culture solution is simply and conveniently separated, and after the separation is finished, the culture solution is filtered again and the supernatant is sucked, so that a good separation effect is achieved, and the quality of the supernatant is ensured.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A centrifuge device for collecting Cyr61 target conditioned media, comprising:
the supporting component (100) comprises a base (110), a shell (120) and a supporting frame (130), the shell (120) is installed on the upper portion of the base (110), the interior of the shell (120) is hollow, and the supporting frame (130) is installed on the upper portion of the shell (120);
the centrifugal assembly (200) comprises a driving motor (210), a connecting shaft (220), a supporting arm (230) and a fixing piece (240), wherein the driving motor (210) is mounted at the upper part of the base (110), the connecting shaft (220) is mounted at the output end of the driving motor (210), the connecting shaft (220) extends into the shell (120), the supporting arm (230) is mounted at the side part of the connecting shaft (220), and the fixing piece (240) is mounted at the upper part of the supporting arm (230);
the filter assembly (300) comprises an electric push rod (310), a connecting frame (320) and a plurality of filter elements (330), wherein the electric push rod (310) is arranged at the upper central part of the support frame (130), the connecting frame (320) is arranged at the output end of the electric push rod (310), the plurality of filter elements (330) are respectively arranged at the lower parts of the connecting frame (320), and the lower parts of the plurality of filter elements (330) are opposite to the upper part of the fixing element (240);
get liquid subassembly (400), it includes suction pump (410), female pipe (420), a plurality of branch pipe (430) and a plurality of suction head (440) to get liquid subassembly (400), suction pump (410) install in the upper portion of support frame (130), female pipe (420) connect in the inlet end of suction pump (410), it is a plurality of branch pipe (430) connect respectively in the inlet end of female pipe (420), it is a plurality of suction head (440) install respectively in the inlet end of a plurality of branch pipe (430), it is a plurality of branch pipe (430) and a plurality of suction head (440) install respectively in the lateral part of link (320), it is a plurality of suction head (440) respectively with a plurality of it is adjacent to filter piece (330).
2. The centrifuge apparatus for collecting Cyr61 target conditioned medium according to claim 1, wherein: the middle part of base (110) is cavity setting, the overall section's of base (110) outline is isosceles trapezoid and sets up.
3. The centrifuge apparatus for collecting Cyr61 target conditioned medium according to claim 1, wherein: the middle part of the shell (120) is provided with a through hole (121) matched with the connecting shaft (220), and the inner wall of the shell (120) is in clearance fit with the side part of the supporting arm (230).
4. The centrifuge apparatus for collecting Cyr61 target conditioned medium according to claim 1, wherein: the fixing piece (240) comprises a supporting plate (241) and a plurality of limiting rings (242), the supporting plate (241) is installed on the upper portion of the supporting arm (230), a plurality of grooves (243) are formed in the upper portion of the supporting plate (241), and the plurality of limiting rings (242) are installed on the inner side, close to the grooves (243), of the supporting plate (241) respectively.
5. The centrifuge apparatus for collecting Cyr61 target conditioned medium according to claim 4, wherein: the center of the supporting disc (241) is coaxial with the center of the connecting shaft (220), and the lower parts of the grooves (243) are respectively opposite to the lower parts of the filter pieces (330).
6. The centrifuge apparatus for collecting Cyr61 target conditioned medium according to claim 1, wherein: filter (330) including adapter sleeve (331), first effect filter screen (332) and high efficiency filter screen (333), adapter sleeve (331) install in the lower part of link (320), first effect filter screen (332) with high efficiency filter screen (333) install respectively in the inboard of adapter sleeve (331), high efficiency filter screen (333) are in the upper portion of first effect filter screen (332).
7. The centrifuge apparatus for collecting Cyr61 target conditioned medium according to claim 6, wherein: the outside of adapter sleeve (331) is glued and is had sealing ring (334), the lower part of sealing ring (334) is provided with guide part (335), the section of guide part (335) is the setting of taper type.
8. The centrifuge apparatus for collecting Cyr61 target conditioned medium according to claim 1, wherein: the main pipe (420) is arranged as a hose.
9. The centrifuge for collecting Cyr61 target conditioned medium according to claim 1, wherein the connecting rack (320) is provided at its side with a pipe clamp (321) engaged with the branch pipe (430), and the inside of the pipe clamp (321) is provided with a flexible pad (322).
10. A centrifugation method of a centrifugation device for collecting Cyr61 target conditioned medium specifically comprises the following steps:
s1, fixing the test tube: placing the culture solution to be centrifuged into a plurality of test tubes, wherein the height of the culture solution is not higher than two thirds of the total height of the test tubes, and then fixing the test tubes on the upper parts of the fixing parts;
s2, culture solution centrifugation: running a driving motor, wherein the driving motor runs to drive a connecting shaft, a supporting arm and a fixing piece to rotate so as to rotate a plurality of test tubes, broken cells are gathered on the side walls of the test tubes under the action of centrifugal force, and then the test tubes are kept stand for 1h-3h so as to gather the broken cells at the bottoms of the test tubes under the action of gravity;
s3, filtering the culture solution: the electric push rod is operated to drive the connecting frame to move downwards, so that the plurality of filtering pieces are inserted into the plurality of test tubes, the culture solution is filtered again in the process of descending the filtering pieces, and the broken cells which do not fall down are filtered to the bottoms of the test tubes;
s4, extracting the supernatant, wherein in the process that the connecting frame moves downwards, the suction head extends into the test tube, then the suction pump is operated, the supernatant on the upper part of the test tube is sucked by the operation of the suction pump, and the supernatant is discharged into an external storage container through the suction head, the branch tube, the mother tube and the suction pump in sequence.
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| CN210543707U (en) * | 2019-05-20 | 2020-05-19 | 湖北锦顺祥环保有限公司 | Reverse suction device of fiber rotary disc filter |
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| CN112034194A (en) * | 2020-09-14 | 2020-12-04 | 安徽新生医学科技有限公司 | Automatic sample feeding device for measuring selenium content of hair based on fluorescence spectrum |
| CN113214970A (en) * | 2021-05-07 | 2021-08-06 | 锦州医科大学 | Self-adaptive adjustment somatic cell culture device and culture method thereof |
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