CN211359217U - High-speed centrifuge for cell separation - Google Patents

High-speed centrifuge for cell separation Download PDF

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Publication number
CN211359217U
CN211359217U CN202021037722.1U CN202021037722U CN211359217U CN 211359217 U CN211359217 U CN 211359217U CN 202021037722 U CN202021037722 U CN 202021037722U CN 211359217 U CN211359217 U CN 211359217U
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China
Prior art keywords
cell separation
speed centrifuge
wall
rack
center pin
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CN202021037722.1U
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Chinese (zh)
Inventor
王保如
王静雪
王立
王永萃
徐大兵
周龙
霍德青
吴挺贵
谢浩杰
石礼森
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Hainan Boao Ruida Medicel International Medical Center Co ltd
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Hainan Boao Ruida Medicel International Medical Center Co ltd
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Abstract

The utility model discloses a high-speed centrifuge is used in cell separation relates to medical cell culture technical field, including the shell, the inner chamber of shell is provided with the center pin, and the upper end outside of center pin is provided with the rack, and the one end of rack is through round pin axle and center pin swing joint, and the lower extreme outside of center pin cup joints and is connected with the ring, the outside fixedly connected with fixed block of ring, and the lower terminal surface middle part of rack has the connecting rod through round pin axle swing joint. The utility model discloses a ring has been cup jointed in the lower extreme outside of center pin, the outside fixedly connected with fixed block of ring, the one end of rack and the lateral wall swing joint of center pin, the both ends of connecting rod respectively with rack and fixed block swing joint, the setting up of ring makes a plurality of racks can rotate in step to guarantee that cell sample is separated the bottom of test tube all the time among the centrifugation process, avoided the possibility that cell sample remixed after the centrifugation finishes.

Description

High-speed centrifuge for cell separation
Technical Field
The utility model relates to a medical treatment cell culture technical field, in particular to high-speed centrifuge is used in cell separation.
Background
The high-speed centrifuge is a machine which separates, concentrates and purifies substances by applying strong centrifugal force according to the difference of the sedimentation coefficient, the mass, the density and the like of the substances, the high-speed centrifuge is widely used in the pharmaceutical field, blood separation, virus research, DNA research, medicine purification and the like are widely used in the high-speed centrifuge, and the centrifugation is to accelerate the sedimentation speed of particles in liquid by utilizing the strong centrifugal force generated by the high-speed rotation of a centrifuge rotor and separate the substances with different sedimentation coefficients and buoyancy densities in a sample. At present, the angle that current cell separation was placed with high speed centrifuge's test tube is fixed, when centrifuge rotational speed increased, the cell sample no longer by separating the test tube bottom, more be the side of being separated the test tube, and after centrifuge stop work, the cell sample mixes together again easily under the action of gravity to seriously influence the separation effect, secondly, the test tube installation that is equipped with the cell sample is not stable enough, leads to among the centrifugal process test tube to break because of bumping easily.
Therefore, it is necessary to develop a high-speed centrifuge for cell separation to solve the above problems.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a high-speed centrifuge is used in cell separation to solve the problem that proposes among the above-mentioned background art.
In order to achieve the above object, the utility model provides a following technical scheme: a high-speed centrifuge for cell separation comprises a shell, wherein a central shaft is arranged in an inner cavity of the shell, a placing frame is arranged on the outer side of the upper end of the central shaft, one end of the placing frame is movably connected with the central shaft through a pin shaft, a circular ring is connected on the outer side of the lower end of the central shaft in a sleeved mode, a fixed block is fixedly connected to the outer side of the circular ring, a connecting rod is movably connected to the middle of the lower end face of the placing frame through a pin shaft, one end of the connecting rod is movably connected with the fixed block through a pin shaft, a placing groove is formed in the upper end face of the placing frame, an extrusion plate is arranged in the inner cavity at one end of the placing groove, a limiting plate is fixedly connected to the upper end of one side face of the extrusion plate, the limiting plate is, one end of the sliding block is fixedly connected with the middle part of the other side surface of the extrusion plate.
Preferably, the placing racks are arranged in a plurality of numbers, and the placing racks are distributed in an annular array.
Preferably, the fixed blocks are arranged in a plurality, and the fixed blocks are distributed on the circular ring in an annular array.
Preferably, the inner wall of the other end of the placing groove is arc-shaped, and a rubber pad is connected to one side surface of the extrusion plate and the lower end surface of the limiting plate in a bonding mode.
Preferably, the inner cavity of one end of the round hole is provided with a thrust spring, and the two ends of the thrust spring are respectively and fixedly connected with the inner wall of one end of the round hole and the other end of the sliding block.
Preferably, the outer side wall of the lower end of the central shaft is provided with a plurality of limiting sliding grooves distributed in an annular array, limiting sliding blocks matched with the limiting sliding grooves are arranged in the limiting sliding grooves, and one ends of the limiting sliding blocks are fixedly connected with the inner wall of the circular ring.
Preferably, the lower extreme of center pin is provided with the backup pad, the backup pad is connected with the inner wall fixed connection of shell, the lower terminal surface middle part fixedly connected with motor of backup pad, the pivot of motor run through the backup pad and with the lower extreme fixed connection of center pin.
The utility model discloses a technological effect and advantage:
1. the utility model discloses a ring has been cup jointed in the lower extreme outside of center pin, the outside fixedly connected with fixed block of ring, the lateral wall swing joint of one end and center pin of rack, the both ends of connecting rod respectively with rack and fixed block swing joint, the setting of ring makes a plurality of racks can rotate synchronously to guarantee that the cell sample is separated to the bottom of test tube all the time in the centrifugation process, avoided the cell sample remixed possibility after the centrifugation finishes;
2. the utility model discloses an inner chamber at the standing groove is provided with the stripper plate, and the inner wall adhesive bonding of standing groove has the rubber pad, a side upper end fixedly connected with limiting plate of stripper plate, and the inner chamber of round hole is provided with thrust spring, and the centrifugal force of this device during operation and thrust spring's thrust make the open end of stripper plate extrusion test tube to the limiting plate can prevent that the test tube from droing from the inner chamber of standing groove, so the installation of test tube is more stable, has avoided the test tube to bump and impaired.
Drawings
Fig. 1 is a schematic view of the overall structure section of the present invention.
Fig. 2 is the ring structure of the present invention is a schematic view of a dive.
Fig. 3 is a schematic view of the structure section of the rack of the present invention.
Fig. 4 is a schematic side view of the extrusion plate structure of the present invention.
In the figure: 1. a housing; 2. a central shaft; 3. placing a rack; 4. a circular ring; 5. a fixed block; 6. a connecting rod; 7. a placement groove; 8. a pressing plate; 9. a limiting plate; 10. a rubber pad; 11. a circular hole; 12. a slider; 13. a thrust spring; 14. a limiting chute; 15. a limiting slide block; 16. a support plate; 17. an electric motor.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
The utility model provides a high-speed centrifuge for cell separation as shown in figures 1-4, as shown in figure 1, comprising a shell 1, wherein the inner cavity of the shell 1 is provided with a central shaft 2, the lower end of the central shaft 2 is provided with a support plate 16, the support plate 16 is fixedly connected with the inner wall of the shell 1, the middle part of the lower end surface of the support plate 16 is fixedly connected with a motor 17, the rotating shaft of the motor 17 penetrates through the support plate 16 and is fixedly connected with the lower end of the central shaft 2, the motor 17 works to drive the central shaft 2 to rotate, the outer side of the upper end of the central shaft 2 is provided with a plurality of placing frames 3, the placing frames 3 are distributed in an annular array, one end of the placing frame 3 is movably connected with the central shaft 2 through a pin shaft, the placing frame 3 can rotate, the outer side of the lower end of the central shaft 2 is sleeved with, fixed block 5 is provided with a plurality ofly, a plurality of fixed blocks 5 are the annular array and distribute on ring 4, there is connecting rod 6 at the lower terminal surface middle part of rack 3 through round pin axle swing joint, the one end of connecting rod 6 is through round pin axle swing joint with fixed block 5, it makes rack 3 from vertical placing tend to the level gradually to place to produce centrifugal force when center pin 2 rotates, and the centrifugal force that rack 3 received is the same with the direction of the resultant force of gravity and rack 3's incline direction, ring 4's setting makes a plurality of racks 3 can rotate in step, the atress is uneven and produce when having avoided this device to rotate at a high speed and rock, standing groove 7 has been seted up to rack 3's up end, standing groove 7 is used.
As shown in fig. 2, a plurality of limiting sliding grooves 14 distributed in an annular array are formed in the outer side wall of the lower end of the central shaft 2, limiting sliding blocks 15 matched with the limiting sliding grooves 14 are arranged in the limiting sliding grooves 14, one ends of the limiting sliding blocks 15 are fixedly connected with the inner wall of the circular ring 4, the limiting sliding grooves 14 are mutually limited by the limiting sliding blocks 15, the circular ring 4 can be prevented from rotating in the outer side of the central shaft 2, the strength of the circular ring 4 can be reinforced by the limiting sliding blocks 15, and the circular ring 4 is prevented from being damaged due to overlarge stress.
As shown in fig. 3 and 4, an extrusion plate 8 is arranged in an inner cavity at one end of a placement groove 7, the inner wall at the other end of the placement groove 7 is arc-shaped, the outer side wall of the bottom of a test tube is attached to the inner wall at the other end of the placement groove 7, therefore, after the test tube is inserted into the inner cavity of the placement groove 7, the bottom of the test tube is limited by the inner wall at the other end of the placement groove 7 and cannot fall off from the inner cavity of the placement groove 7, the stress area of the bottom of the test tube is increased, and the test tube can be effectively prevented from being broken due to concentrated stress at a certain point at the bottom of the test tube, a limiting plate 9 is fixedly connected to the upper end of one side surface of the extrusion plate 8, the limiting plate 9 is obliquely arc-shaped, the lower end surface of the limiting plate 9 is attached to the outer side wall of the open, rubber pad 10's setting can prevent that the test tube wall from receiving the collision and impaired, round hole 11 has been seted up to the one end inner wall of standing groove 7, be provided with in the round hole 11 with the sliding block 12 of round hole 11 looks adaptation, sliding block 12's one end and stripper plate 8's another side middle part fixed connection, round hole 11's one end inner chamber is provided with thrust spring 13, thrust spring 13's both ends respectively with round hole 11's one end inner wall and sliding block 12's other end fixed connection, thrust spring 13 provides thrust and makes the open end of stripper plate 8's a side extrusion test tube all the time, thereby make the installation of test tube more stable, and this device during operation, stripper plate 8 receives the dual function of centrifugal force and thrust spring 13 thrust, therefore this device rotational speed is faster, stripper plate 8 is bigger to the extrusion force.
The utility model discloses the theory of operation:
before the device is used, the extrusion plate 8 is slid, then the test tube is placed in the inner cavity of the placement groove 7, the extrusion plate 8 is loosened, the thrust spring 13 provides thrust to enable one side face of the extrusion plate 8 to extrude the open end of the test tube, and the lower end face of the limiting plate 9 is attached and connected with the outer side wall of the open end of the test tube, so that the test tube can be stably installed in the inner cavity of the placement groove 7;
this device during operation, starter motor 17, motor 17 work drive center pin 2 rotates, and the centrifugal force that produces when center pin 2 rotates makes rack 3 tend to the horizontality by vertical state gradually to the centrifugal force that rack 3 received is the same with the incline direction of rack 3 with the resultant force direction of gravity, and the setting up of ring 4 makes a plurality of racks 3 can rotate in step, and the atress is uneven and produces and rock when having avoided this device to rotate at a high speed.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims (7)

1. A high-speed centrifuge for cell separation, comprising a casing (1), characterized in that: the inner cavity of the shell (1) is provided with a central shaft (2), the outer side of the upper end of the central shaft (2) is provided with a placing frame (3), one end of the placing frame (3) is movably connected with the central shaft (2) through a pin shaft, the outer side of the lower end of the central shaft (2) is connected with a circular ring (4) in a sleeved mode, the outer side of the circular ring (4) is fixedly connected with a fixed block (5), the middle part of the lower end face of the placing frame (3) is movably connected with a connecting rod (6) through a pin shaft, one end of the connecting rod (6) is movably connected with the fixed block (5) through a pin shaft, the upper end face of the placing frame (3) is provided with a placing groove (7), an inner cavity at one end of the placing groove (7) is provided with an extrusion plate (8), the, the inner wall adhesive joint of standing groove (7) has rubber pad (10), round hole (11) have been seted up to the one end inner wall of standing groove (7), be provided with in round hole (11) with round hole (11) looks adaptation sliding block (12), the one end of sliding block (12) and the another side middle part fixed connection of stripper plate (8).
2. The high-speed centrifuge for cell separation according to claim 1, wherein: the rack (3) is provided with a plurality of racks, and the racks (3) are distributed in an annular array.
3. The high-speed centrifuge for cell separation according to claim 2, wherein: the fixed blocks (5) are arranged in a plurality, and the fixed blocks (5) are distributed on the circular ring (4) in an annular array.
4. The high-speed centrifuge for cell separation according to claim 3, wherein: the inner wall of the other end of the placing groove (7) is arc-shaped, and a rubber pad (10) is connected to one side face of the extrusion plate (8) and the lower end face of the limiting plate (9) in an adhering mode.
5. The high-speed centrifuge for cell separation according to claim 4, wherein: the inner cavity of one end of the round hole (11) is provided with a thrust spring (13), and two ends of the thrust spring (13) are fixedly connected with the inner wall of one end of the round hole (11) and the other end of the sliding block (12) respectively.
6. The high-speed centrifuge for cell separation according to claim 5, wherein: the lower end outer side wall of the central shaft (2) is provided with a plurality of limiting sliding grooves (14) distributed in an annular array mode, limiting sliding blocks (15) matched with the limiting sliding grooves (14) are arranged in the limiting sliding grooves (14), and one ends of the limiting sliding blocks (15) are fixedly connected with the inner wall of the circular ring (4).
7. The high-speed centrifuge for cell separation according to claim 6, wherein: the lower extreme of center pin (2) is provided with backup pad (16), the inner wall fixed connection of backup pad (16) and shell (1), the lower terminal surface middle part fixedly connected with motor (17) of backup pad (16), the pivot of motor (17) run through backup pad (16) and with the lower extreme fixed connection of center pin (2).
CN202021037722.1U 2020-06-09 2020-06-09 High-speed centrifuge for cell separation Active CN211359217U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202021037722.1U CN211359217U (en) 2020-06-09 2020-06-09 High-speed centrifuge for cell separation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202021037722.1U CN211359217U (en) 2020-06-09 2020-06-09 High-speed centrifuge for cell separation

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112588457A (en) * 2020-11-30 2021-04-02 北京图腾猎技科技有限公司 Cell engineering is with continuous efficient splitter
CN114405691A (en) * 2021-12-26 2022-04-29 山东维尤纳特生物科技有限公司 Centrifugal equipment for producing metaldehyde

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112588457A (en) * 2020-11-30 2021-04-02 北京图腾猎技科技有限公司 Cell engineering is with continuous efficient splitter
CN112588457B (en) * 2020-11-30 2021-10-26 湖南康润药业股份有限公司 Cell engineering is with continuous efficient splitter
CN114405691A (en) * 2021-12-26 2022-04-29 山东维尤纳特生物科技有限公司 Centrifugal equipment for producing metaldehyde

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