CN113940974A

CN113940974A - Traditional Chinese medicine composition for treating cancer, preparation method and application

Info

Publication number: CN113940974A
Application number: CN202111374025.4A
Authority: CN
Inventors: 王泽达
Original assignee: Wang Zeda
Current assignee: Wang Zeda
Priority date: 2021-11-19
Filing date: 2021-11-19
Publication date: 2022-01-18

Abstract

The invention belongs to the technical field of traditional Chinese medicine compositions, and discloses a traditional Chinese medicine composition for treating cancer, a preparation method and an application thereof, wherein the traditional Chinese medicine composition for treating cancer comprises, by mass, 20-30 parts of pseudo-ginseng, 20-30 parts of chrysanthemum morifolium, 15-20 parts of tricholoma matsutake, 15-20 parts of aloe, 6-8 parts of loofah sponge, 6-8 parts of edible tulip, 6-8 parts of selfheal, 3-5 parts of blackberry lily, 2-3 parts of almond and 0.1-05 parts of centipede. By adding various anti-cancer Chinese herbal medicines, the growth of cancer cells can be effectively inhibited, and the metastasis and transformation of the cancer cells can be controlled; the invention can effectively relieve the pain of cancer patients, treat and relieve the symptoms of cancer, and has good treatment effect and low cost. The invention adopts natural Chinese herbal medicines, has high safety, does not cause damage to human bodies, has no other side effects and has simple production process.

Description

Traditional Chinese medicine composition for treating cancer, preparation method and application

Technical Field

The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a traditional Chinese medicine composition for treating cancer, a preparation method and application thereof.

Background

At present, cancer is one of common diseases, wherein breast cancer, colon cancer and rectal cancer have the characteristic of high morbidity in cancer, the cure rate of the existing medicines is low, the existing medicines are easy to repeatedly attack and high in treatment cost when the existing medicines are used for treating the cancer, the treatment effect is poor when the existing medicines are mainly used for clinically treating the cancer through operations, chemotherapy and the like, and great pain is brought to patients. In addition, for fibrous cancer on the body surface and cyst inside and outside the body, the modern therapies mostly adopt surgical treatment and chemotherapy at present, and have not only great pain for patients, but also unsatisfactory effect and heavy economic cost.

Through the above analysis, the problems and defects of the prior art are as follows: the existing traditional Chinese medicine composition for treating cancer has poor effect; and other treatment methods have high cost, poor experience of patients and great side effect.

Disclosure of Invention

Aiming at the problems in the prior art, the invention provides a traditional Chinese medicine composition for treating cancer, a preparation method and application thereof.

The traditional Chinese medicine composition for treating the cancer is prepared from 20-30 parts of pseudo-ginseng, 20-30 parts of chrysanthemum morifolium, 15-20 parts of tricholoma matsutake, 15-20 parts of aloe, 6-8 parts of loofah sponge, 6-8 parts of edible tulip, 6-8 parts of selfheal, 3-5 parts of blackberry lily, 2-3 parts of almond and 0.1-05 parts of centipede in parts by mass.

Another object of the present invention is to provide a method for preparing a cancer-treating Chinese medicinal composition, which comprises the following steps:

weighing pseudo-ginseng, chrysanthemum morifolium, tricholoma matsutake, aloe, loofah sponge, edible tulip, selfheal, blackberry lily, almond and centipede according to a proportion;

step two, pretreating the weighed edible tulip, and performing supercritical extraction and dispersion by using carbon dioxide to obtain a colchicine extract;

step three, preparing a panax notoginseng nano dispersion liquid by cleaning, drying, crushing, extracting and homogenizing the weighed panax notoginseng;

step four, carrying out pretreatment, extraction, separation and purification on the weighed prunella vulgaris to obtain a prunella vulgaris extract;

step five, cleaning, drying, pretreating and crushing Hangzhou chrysanthemum, matsutake mushroom, loofah sponge, blackberry lily, almond and centipede to obtain mixed powder;

and step six, stirring and uniformly mixing the mixed powder, the prepared colchicine extract, the nano dispersion liquid, the prunella vulgaris extract and the aloe, performing ultrasonic extraction, homogenizing and sterilizing to obtain the traditional Chinese medicine composition for treating cancers.

Further, in the second step, the step of pretreating the weighed iphigenia indica and performing supercritical extraction and dispersion by using carbon dioxide to obtain a colchicine extract comprises the following steps:

firstly, washing the weighed edible tulip with flowing water for 2-3 times, and naturally drying; carrying out high-pressure treatment on the air-dried iphigenia indica by using a high-pressure rotary cooker;

secondly, performing microwave treatment on the high-pressure treated iphigenia indica; obtaining processed edible tulip;

finally, performing carbon dioxide supercritical extraction on the treated iphigenia indica to obtain a primary colchicine extract; and performing nano-dispersion treatment on the initial colchicine extract to obtain the colchicine extract.

Further, the high pressure processing comprises: treated at 4MPa for 1 hour.

Further, the microwave treatment comprises: treating for 20 minutes under 2-3KW microwave.

Further, in the third step, the preparation of the notoginseng nano dispersion liquid by washing, drying, crushing, extracting and homogenizing the weighed notoginseng comprises:

cleaning and drying pseudo-ginseng, and crushing by using a crusher to obtain pseudo-ginseng fragments; treating the pseudo-ginseng fragments by using high-temperature water vapor to obtain pseudo-ginseng volatile oil and dregs;

adding a certain amount of ethanol into the dregs of a decoction for carrying out ethanol extraction to obtain an extracting solution; concentrating the extracting solution to obtain a pseudo-ginseng concentrated paste;

mixing the radix Notoginseng concentrated paste with radix Notoginseng volatile oil, and homogenizing under dynamic ultrahigh pressure micro-jet to obtain radix Notoginseng nanometer dispersion.

Further, the ethanol concentration was 65%.

Further, in the fourth step, the step of preprocessing, extracting, separating and purifying the weighed prunella vulgaris to obtain the prunella vulgaris extract comprises the following steps:

soaking the selfheal in clear water for 2-3 minutes, and then drying in the sun in the burning sun; and crushing the dried selfheal, and sieving the crushed selfheal with a sieve of 20 to 30 meshes;

sterilizing the crushed prunella vulgaris at high temperature, and simultaneously performing ultrasonic extraction on the sterilized prunella vulgaris; adding an adsorbent into the extracting solution for decoloring, and filtering to remove the adsorbent;

and (4) separating and purifying the decolorized selfheal extracting solution to obtain a purified selfheal extract.

Further, the separation and purification treatment of the decolorized prunella vulgaris extract comprises the following steps:

extracting the decolorized prunella vulgaris extract by using petroleum ether to obtain prunella vulgaris extract;

concentrating the selfheal extract, passing through a silica gel column, and eluting by using a mixed solvent of ethyl acetate and normal hexane to obtain a selfheal extract;

adding water into Prunellae Spica extract to obtain suspension, adsorbing with macroporous resin to saturation, eluting with water to remove impurities and solvent, recovering solvent from the eluate under reduced pressure, and vacuum concentrating to obtain purified Prunellae Spica extract.

Further, the silica gel column is 200-280 meshes.

The invention also aims to provide application of the traditional Chinese medicine composition for treating cancer in preparation of capsule medicines or liquid medicine medicines for treating and relieving cancer.

By combining all the technical schemes, the invention has the advantages and positive effects that: by adding various anti-cancer Chinese herbal medicines, the growth of cancer cells can be effectively inhibited, and the metastasis and transformation of the cancer cells can be controlled; the invention can effectively relieve the pain of cancer patients, treat and relieve the symptoms of cancer, and has good treatment effect and low cost. The invention adopts natural Chinese herbal medicines, has high safety, does not cause damage to human bodies, has no other side effects and has simple production process.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.

Fig. 1 is a flow chart of a method for preparing a Chinese medicinal composition for treating cancer according to an embodiment of the present invention.

Fig. 2 is a flow chart of a method for pretreating weighed edible tulip bulb, and performing supercritical carbon dioxide extraction and dispersion to obtain a colchicine extract, according to an embodiment of the present invention.

Fig. 3 is a flow chart of a method for preparing a notoginseng nano dispersion by washing, drying, crushing, extracting and homogenizing weighed notoginseng provided by the embodiment of the invention.

Fig. 4 is a flow chart of a method for obtaining a prunella vulgaris extract by pretreating, extracting, separating and purifying the weighed prunella vulgaris according to an embodiment of the present invention.

FIG. 5 is a flow chart of a method for separating and purifying a decolorized Prunella vulgaris extract according to an embodiment of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Aiming at the problems in the prior art, the invention provides a traditional Chinese medicine composition for treating cancer and a preparation method thereof, and the invention is described in detail with reference to the accompanying drawings.

The traditional Chinese medicine composition for treating cancer provided by the embodiment of the invention comprises, by mass, 20-30 parts of pseudo-ginseng, 20-30 parts of chrysanthemum morifolium, 15-20 parts of tricholoma matsutake, 15-20 parts of aloe, 6-8 parts of loofah sponge, 6-8 parts of edible tulip, 6-8 parts of selfheal, 3-5 parts of blackberry lily, 2-3 parts of almond and 0.1-05 parts of centipede.

As shown in fig. 1, the preparation method of the traditional Chinese medicine composition for treating cancer provided by the embodiment of the invention comprises the following steps:

s101, weighing pseudo-ginseng, chrysanthemum morifolium, tricholoma matsutake, aloe, loofah sponge, edible tulip, selfheal, blackberry lily, almond and centipede according to a proportion;

s102, pretreating the weighed edible tulip, and performing supercritical extraction and dispersion by using carbon dioxide to obtain a colchicine extract;

s103, preparing a panax notoginseng nano dispersion liquid by cleaning, drying, crushing, extracting and homogenizing the weighed panax notoginseng;

s104, preprocessing, extracting, separating and purifying the weighed prunella vulgaris to obtain a prunella vulgaris extract;

s105, cleaning, drying, pretreating and crushing Hangzhou chrysanthemum, matsutake mushroom, loofah sponge, blackberry lily, almond and centipede to obtain mixed powder;

s106, stirring and uniformly mixing the mixed powder, the prepared colchicine extract, the nano dispersion liquid, the prunella vulgaris extract and the aloe, performing ultrasonic extraction, homogenizing and sterilizing to obtain the traditional Chinese medicine composition for treating the cancer.

As shown in fig. 2, the pretreatment of the weighed iphigenia indica, supercritical carbon dioxide extraction, and dispersion to obtain the colchicine extract according to the embodiment of the present invention includes:

s201, washing the weighed iphigenia indica with flowing water for 2-3 times, and naturally drying; treating the air-dried edible tulip for 1 hour at 4MPa by using a pressure rotary cooker;

s202, treating the high-pressure treated iphigenia indica with 2-3KW of microwave for 20 minutes; obtaining processed edible tulip;

s203, performing carbon dioxide supercritical extraction on the processed edible tulip to obtain a primary colchicine extract; and performing nano-dispersion treatment on the initial colchicine extract to obtain the colchicine extract.

As shown in fig. 3, the preparation of the notoginseng nano dispersion liquid by washing, drying, pulverizing, extracting and homogenizing the weighed notoginseng provided by the embodiment of the present invention includes:

s301, cleaning and drying pseudo-ginseng, and crushing the pseudo-ginseng by using a crusher to obtain pseudo-ginseng fragments; treating the pseudo-ginseng fragments by using high-temperature water vapor to obtain pseudo-ginseng volatile oil and dregs;

s302, adding a certain amount of ethanol into the medicine dregs for ethanol extraction to obtain an extracting solution; concentrating the extracting solution to obtain a pseudo-ginseng concentrated paste;

s303, mixing the panax notoginseng concentrated paste with the panax notoginseng volatile oil, and homogenizing by dynamic ultrahigh pressure microjet to obtain a panax notoginseng nano dispersion liquid.

The ethanol concentration provided by the embodiment of the invention is 65%.

As shown in fig. 4, the obtaining of the prunella vulgaris extract by pretreating, extracting, separating and purifying the weighed prunella vulgaris according to the embodiment of the present invention includes:

s401, soaking the selfheal in clear water for 2-3 minutes, and drying in the sun in the burning sun; and crushing the dried selfheal, and sieving the crushed selfheal with a sieve of 20 to 30 meshes;

s402, sterilizing the crushed prunella vulgaris at high temperature, and simultaneously performing ultrasonic extraction on the sterilized prunella vulgaris; adding an adsorbent into the extracting solution for decoloring, and filtering to remove the adsorbent;

and S403, separating and purifying the decolorized selfheal extracting solution to obtain a purified selfheal extract.

As shown in fig. 5, the separation and purification treatment of the decolorized prunella vulgaris extract according to the embodiment of the present invention includes:

s501, extracting the decolorized selfheal extracting solution by using petroleum ether to obtain selfheal extracting solution;

s502, concentrating the selfheal extract, passing through a silica gel column, and eluting by using a mixed solvent of ethyl acetate and normal hexane to obtain a selfheal extract;

s503, adding water into the selfheal extract to prepare a suspension, adsorbing the suspension to saturation through macroporous resin, eluting impurities and a solvent with water again, recovering the solvent from the eluate under reduced pressure, and concentrating in vacuum to obtain the purified selfheal extract.

The silica gel column provided by the embodiment of the invention is 200-280 meshes.

The technical solution of the present invention will be further described with reference to the following embodiments.

Example 1

The traditional Chinese medicine composition for treating cancer provided by the embodiment of the invention comprises 20-30 parts of pseudo-ginseng, 20 parts of chrysanthemum morifolium, 15 parts of tricholoma matsutake, 15 parts of aloe, 6 parts of loofah sponge, 6 parts of edible tulip, 6 parts of selfheal, 3 parts of blackberry lily, 2 parts of almond and 0.1 part of centipede in parts by mass.

Example 2

The traditional Chinese medicine composition for treating cancer provided by the embodiment of the invention comprises 30 parts of pseudo-ginseng, 30 parts of chrysanthemum morifolium, 20 parts of tricholoma matsutake, 20 parts of aloe, 8 parts of loofah sponge, 8 parts of edible tulip, 8 parts of selfheal, 5 parts of blackberry lily, 3 parts of almond and 05 parts of centipede in parts by mass.

Example 3

The traditional Chinese medicine composition for treating cancer provided by the embodiment of the invention comprises, by mass, 25 parts of pseudo-ginseng, 25 parts of chrysanthemum morifolium, 17.5 parts of tricholoma matsutake, 17.5 parts of aloe, 7 parts of loofah sponge, 7 parts of edible tulip, 7 parts of selfheal, 4 parts of blackberry lily, 2.5 parts of almond and 0.3 part of centipede.

The technical effects of the present invention will be further described below in conjunction with pharmacological analysis.

Notoginseng radix is dried root of Panax notoginseng (Burk.) F.H.Chen of Araliaceae. Has effects of removing blood stasis, stopping bleeding, promoting blood circulation, and relieving pain. It can be used for treating hemorrhage, traumatic injury, and swelling and pain due to blood stasis.

Hangzhou chrysanthemum, sweet, bitter and slightly cold, enters lung and liver channels, has the efficacies of dispelling wind and heat, nourishing liver, improving eyesight, clearing heat and removing toxicity, and has the following uses: affection of exogenous wind-heat, fever and headache: it is often combined with herbs such as sang Ye and Bo He; it is combined with Huang Qin and Zhi Zi to treat restlessness due to excessive heat. For blood-shot eyes with swelling and pain, it is often combined with Chan Yi and Jue Ming Zi; for dim eyes due to liver yang deficiency, it is often combined with Di Huang, shan Yu Rou and Gou Qi Zi. Carbuncle, deep-rooted carbuncle, sore and ulcer, red swelling, heat pain, furuncle and pyogenic infections: it is often combined with jin Yin Hua and gan Cao. Headache, fullness in the head, dizziness, blurred vision due to hyperactivity of liver-yang: it is often combined with Bai Shao, Gou Teng and Zhen Zhu mu.

The tricholoma matsutake has a series of effects of tonifying kidney, strengthening essence, restoring physical strength, tonifying stomach, tonifying qi, strengthening heart, enriching blood, strengthening brain, improving intelligence, regulating qi, reducing phlegm and the like. The tricholoma matsutake can be used as food materials clinically to achieve the effect of food and medicine supplementation, such as resisting malignant tumors, resisting radiation and aging, and has great significance for improving the immunity of human bodies, maintaining beauty and keeping young and regulating the internal environment of heart and cerebral vessels. The oral administration of the tricholoma matsutake in daily life has great benefits for preventing diseases, particularly has great significance for preventing malignant tumors and resisting cell mutation, and can also have the effect of preventing chronic diseases such as coronary heart disease, cerebral thrombosis and the like through the tricholoma matsutake in clinic.

Aloe, function and use of Aloe are rich in nicotinic acid, vitamin B6, etc., are bitter stomach invigorating and laxative agents, have effects of resisting inflammation, repairing gastric mucosa and relieving pain, are favorable for the treatment of gastritis, gastric ulcer, and can promote healing of ulcer.

Loofah sponge, the name of traditional Chinese medicine. Is the vascular bundle of dried mature fruit of Luffa cylindrica (L.) Roem. Picking in summer and autumn when fruit is mature, pericarp turns yellow, and interior is dry, removing outer skin and pulp, cleaning, sun drying, and removing seed. Can be used for treating arthralgia, spasm, distending pain of chest and hypochondrium, galactostasis, and mammary abscess with swelling and pain.

Pseudobulbus Cremastrae Seu pleiones has effects of removing toxic substance, eliminating phlegm, and relieving cough. Firstly, it has the action of clearing heat and resolving masses. Clinical observation shows that the edible tulip can gradually disappear after being taken for a long time for the cervical lymph nodes, and the edible tulip is proved to have the functions of clearing heat, softening hardness and resolving masses. Second, the edible tulip has the functions of detoxification, phlegm reduction and cough relief. Thirdly, the edible tulip can also improve the immunity of the organism. A nonspecific substance in the polysaccharide in the edible tulip stimulates a reticuloendothelial system, so that the immunoreaction capability of a host to a cancer cell specific antigen is improved, and the anticancer effect of an organism is exerted. In addition, Pseudobulbus Cremastrae Seu pleiones can enhance or prolong the action of hypnotic agent, increase sensitivity of central inhibitory drug, inhibit respiratory center, increase reverse sympathetic nervous response, excite vasomotor center, and increase blood pressure. Has irritation effect on gastrointestinal mucosa, and can cause hemorrhage, even hemorrhagic gastroenteritis. It should be noted that Pseudobulbus Cremastrae seu pleiones should be used with cautions for those with weak constitution. Excessive amount of Pseudobulbus Cremastrae Seu pleiones can cause poisoning, and long-term administration can cause adverse reaction of gastrointestinal tract, polyneuritis, leukopenia, etc.

Spica Prunellae has effects and actions mainly reflected in the treatment and symptom relief of lymph node and thyroid enlargement, breast cancer, dizziness, facial distortion, pulmonary tuberculosis, acute icterohepatitis, arthralgia and myalgia, etc.

The efficacies of the blackberry lily are clearing away heat and toxic material, eliminating phlegm and relieving sore throat. In clinic, blackberrylily rhizome is often combined with ephedra and the like to form blackberrylily rhizome ephedra decoction for treating lung diseases. In daily application, the blackberrylily rhizome can treat sore throat and profuse sputum, especially yellow sputum, caused by wind heat, chronic rhinitis caused by stuffy nose, obstructive diseases such as constipation and the like, and can be used for adjusting abdominal dropsy, hernia and mastitis. The dose of the blackberrylily rhizome is less than 10 g each time, the blackberrylily rhizome has certain toxicity, is particularly bitter and cold and is not suitable for pregnant women, and the application is carried out under the guidance of doctors. Belamcanda rhizome, bitter in flavor and cold in flavor, enters lung meridian and has the main effects of clearing heat and removing toxicity, dissolving phlegm and relieving sore throat.

The almond has the effects of depressing qi, relieving cough and asthma and relaxing bowel. Apricot kernel, semen Armeniacae amarum, slightly warm in nature and bitter in taste, enters lung and large intestine meridians.

Centipede, belonging to the poisonous insects, can be used as a medicinal material and is warm in nature. The Chinese medicinal composition has the effects similar to the effects of honeysuckle, dandelion and other medicaments, has the effects of clearing heat and removing toxicity, promoting blood circulation and removing blood stasis and the like, has a good effect on treating traumatic injuries, also has the effects of resisting bacteria and tumors, can promote the immunologic function of a human body, can be used for treating rheumatism and obstinate arthralgia, and also has the effect on treating headache.

Clinical tests show that the traditional Chinese medicine composition and the medicament for treating the cancer provided by the invention can be used for preventing and treating the cancer and have obvious treatment effects.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, and any modification, equivalent replacement, and improvement made by those skilled in the art within the technical scope of the present invention disclosed herein, which is within the spirit and principle of the present invention, should be covered by the present invention.

Claims

1. The traditional Chinese medicine composition for treating the cancer is characterized by comprising, by mass, 20-30 parts of pseudo-ginseng, 20-30 parts of chrysanthemum morifolium, 15-20 parts of tricholoma matsutake, 15-20 parts of aloe, 6-8 parts of loofah sponge, 6-8 parts of edible tulip, 6-8 parts of selfheal, 3-5 parts of blackberry lily, 2-3 parts of almond and 0.1-05 parts of centipede.

2. The method for preparing the cancer-treating traditional Chinese medicine composition according to claim 1, wherein the method for preparing the cancer-treating traditional Chinese medicine composition comprises the following steps:

3. The method for preparing the Chinese medicinal composition for treating cancer according to claim 2, wherein in the second step, the step of pretreating the weighed iphigenia indica, and performing supercritical carbon dioxide extraction and dispersion to obtain the colchicine extract comprises the following steps:

4. The method of preparing a herbal composition for cancer treatment of claim 3, wherein the high pressure treatment comprises: treated at 4MPa for 1 hour.

5. The method for preparing a Chinese medicinal composition for treating cancer according to claim 3, wherein the microwave treatment comprises: treating for 20 minutes under 2-3KW microwave.

6. The method for preparing the traditional Chinese medicine composition for treating cancer as claimed in claim 2, wherein the step three, the preparation of the notoginseng nano dispersion liquid by washing, drying, crushing, extracting and homogenizing the weighed notoginseng, comprises:

7. The method of preparing a pharmaceutical composition according to claim 6, wherein the concentration of ethanol is 65%.

8. The method for preparing a Chinese medicinal composition for treating cancer according to claim 2, wherein in the fourth step, the step of obtaining the prunella vulgaris extract by pretreating, extracting, separating and purifying the weighed prunella vulgaris comprises:

9. The method for preparing a Chinese medicinal composition for treating cancer according to claim 8, wherein the separation and purification treatment of the decolorized Prunella vulgaris extract comprises:

concentrating the selfheal extract, passing through a 200-mesh 280-mesh silica gel column, and eluting by using a mixed solvent of ethyl acetate and n-hexane to obtain a selfheal extract;

10. The use of the cancer treatment composition of claim 1 in the preparation of a capsule or liquid medicament for the treatment or alleviation of cancer.