CN113940963B

CN113940963B - Exocarpium citri grandis product and preparation method and application thereof

Info

Publication number: CN113940963B
Application number: CN202111238743.9A
Authority: CN
Inventors: 蔡伟茂; 简美妨
Original assignee: Huazhou Caijiayuan Chemical Orange Co ltd
Current assignee: Huazhou Caijiayuan Chemical Orange Co ltd
Priority date: 2021-10-25
Filing date: 2021-10-25
Publication date: 2023-01-13
Anticipated expiration: 2041-10-25
Also published as: CN113940963A

Abstract

The invention relates to the technical field of traditional Chinese medicines, and in particular relates to a pummelo peel product and a preparation method and application thereof. The preparation method of the exocarpium Citri Grandis product comprises treating exocarpium Citri Grandis at low temperature, wherein the temperature of the whole treatment process is not more than 45 deg.C. The invention adopts a low-temperature treatment process, the temperature of the treatment process is controlled not to exceed 45 ℃ in the whole process, so that the lipid substance in the pummelo peel has stable structure and stronger medicinal efficacy of reducing phlegm, relieving cough, benefiting qi and relieving asthma. The prepared exocarpium Citri Grandis product has certain fragrance and persistent pungent and anesthetic taste, and after long-term storage, the fragrance and pungent and anesthetic taste of exocarpium Citri Grandis are not reduced.

Description

Exocarpium citri grandis product and preparation method and application thereof

Technical Field

The invention relates to the technical field of traditional Chinese medicines, and in particular relates to a pummelo peel product and a preparation method and application thereof.

Background

Exocarpium Citri Grandis, also known as exocarpium Citri Grandis and exocarpium Citri Grandis. The tea is warm, bitter and pungent in nature, contains various chemical components such as alkaloid, flavonoid, polysaccharide and volatile oil, has the effects of reducing phlegm and relieving cough, regulating qi and relieving asthma, resolving food stagnation and promoting digestion and the like, and has unique curative effects on treating diseases such as cold cough, chronic cough, bronchitis, pharyngolaryngitis, asthma, food stagnation and alcohol injury. The Ming Dynasty's pharmaceutical famous book Ben Cao gang mu' is advocated to it and called as "good Juhong article of orange", its flesh has red and white components, which is better than other drugs in the effects of benefiting qi, resolving phlegm and relieving cough, … …, chen Yuliang is cured. Qing dynasty also had the reputations of "true, one-golden and" southern ginseng ", which are listed as palace tributes.

The method comprises collecting exocarpium Citri Grandis in summer when the fruit is immature, blanching in boiling water, cutting pericarp into 5 or 7 pieces, removing fruit pulp and part of mesocarp, press-forming, and drying. However, the Chinese pharmacopoeia only takes the naringin content as a basis, neglects the important function of the pungent-warm volatile oil and oil substances, and the citrus grandis prepared by the provided method has poor drug effect, mediocre phlegm-resolving and cough-relieving efficacy, mediocre qi-benefiting and asthma-relieving efficacy, and other traditional Chinese medicines for resolving phlegm-resolving and cough-relieving and qi-benefiting and asthma-relieving need to be added to enhance the efficacy, so that the chronic bronchitis disease and the chronic obstructive pulmonary disease cannot be treated better. The Chinese invention patent CN105726723A discloses pummelo peel decoction pieces and a processing method thereof, wherein parameters influencing the quality of the final finished product of the pummelo peel decoction pieces, such as drying temperature, drying time, enzyme deactivation time, cutting size and the like in the processing method of the pummelo peel decoction pieces are optimally selected, so that the pummelo peel decoction pieces with attractive and uniform appearance and high effective components are obtained. However, the method focuses on the production cycle and the flavor evaluation of the pummelo peel drink, only relatively increases the contents of the sample oleandrin and limonene, and cannot ensure the medicinal efficacy of the pummelo peel.

Therefore, the preparation method standards of the pummelo peel in the current market are not uniform, the quality difference of products with various specifications is large, and the efficacy of the pummelo peel or pummelo peel products circulated in the market is mediocre and can not be used greatly, so that the real value of the pummelo peel is difficult to be exerted.

Disclosure of Invention

In view of the above, there is a need to provide a pummelo peel product, and a preparation method and application thereof. The drug property effect of the pummelo peel is improved by regulating and controlling the collection period of the pummelo peel, the screening and cultivation of varieties, the preparation process and other influencing factors.

In order to realize the purpose, the invention adopts the following technical scheme:

in a first aspect, the invention provides a preparation method of a pummelo peel product, which is to treat the outer peel of pummelo peel at a low temperature, wherein the temperature of the low temperature is not more than 45 ℃ in the whole treatment process.

Further, in the preparation method of the exocarpium citri grandis product, the specific mode is that the exocarpium citri grandis is washed by cold water, all flesh is removed, outer peel is peeled off, and the outer peel is dried and ground into powder.

Further, in the above method for preparing a exocarpium Citri Grandis product, the drying manner includes, but is not limited to, airing, sun drying, oven drying or air drying.

Further, in the above method for producing a exocarpium Citri Grandis product, the fineness of the milled powder is 200 mesh or more. The exocarpium citri grandis product prepared by the preparation method disclosed by the invention is high in oil content, the powder is in a small lump shape, and if the content is lower than 200 meshes, manual sieving is difficult.

Furthermore, in the preparation method of the pummelo peel product, pummelo peel powder is extracted by water for 1 to 3 times, and water extracting solutions are combined and concentrated into extraction paste.

Further, in the above method for preparing a pummelo peel product, the pummelo peel powder obtained by milling is extracted with water for 1 to 3 times, and the water extract is combined and then separated and purified with an organic solvent.

Further, in the preparation method of the pummelo peel product, the pummelo peel is harvested in a mature period.

Further, in the above method for producing a pummelo peel product, the harvesting in the mature period is pummelo peel harvested in winter every year or pummelo peel harvested in spring next year.

Further, in the above preparation method of the pummelo peel product, the pummelo peel is a superior pure pummelo peel; the superior product is exocarpium Citri Grandis with navel bottom or exocarpium Citri Grandis with golden bell shape; the pure breed is pure breed gene exocarpium citri grandis which is bred by always bearing the branches in history or by taking the seed seedlings of the exocarpium citri grandis which are bred by always bearing the branches as stocks and taking bud pieces as scion buds. The original wild property of the high-quality pure pummelo peel is sufficient, and the scion bud joint has the characteristic of producing the longest spine.

In a second aspect, the invention provides a pummelo peel product which is prepared by adopting the preparation method.

Further, in the above-mentioned exocarpium Citri Grandis product, the product form includes, but is not limited to, powder, granule, paste, tablet or capsule.

In a third aspect, the invention provides a traditional Chinese medicine preparation, which comprises the pummelo peel product.

Further, the traditional Chinese medicine preparation also comprises other traditional Chinese medicines and auxiliary materials, wherein the other traditional Chinese medicines include but are not limited to astragalus membranaceus, angelica sinensis, cordyceps sinensis, adenophora stricta, honeysuckle and chrysanthemum.

In a fourth aspect, the invention provides a preparation method of the pummelo peel product, the pummelo peel product and application of a traditional Chinese medicine preparation in preparation of medicines for treating respiratory diseases.

The invention has the beneficial effects that:

compared with the processing mode of processing the whole fruits or the claw pieces of the pummelo peel by using a fire drying or electric drying mode at 50-90 ℃ in the prior art, the invention adopts a low-temperature processing technology, controls the temperature of the processing technology to be not more than 45 ℃ in the whole process, and ensures that the grease substance in the pummelo peel has stable structure and stronger drug property and efficacy. The prepared exocarpium Citri Grandis product has certain fragrance and persistent pungent and anesthetic taste, and after 3 years of long-term storage, the fragrance and pungent and anesthetic taste of exocarpium Citri Grandis are not reduced. Also avoids the defect that the high temperature changes the warm property into the dry heat property, and is no longer suitable for wind-heat cough, and is beneficial to the initial treatment of acute bronchitis.

In the prior art, the non-mature exocarpium citri rubrum picked in summer takes the naringin content as the picking basis, and the important function of obtaining more pungent-warm volatile oil and fat substances can be obtained only by neglecting the mature period. The invention adopts the pummelo peel in the mature period, can greatly improve the aromaticity in the epicarp, and the oil and the volatile oil Xin Wenlei matter comprehensively and evenly improve the temperature, the bitterness and the Xin Yaoxing, thicken the unique smell of the pummelo peel, thereby improving the efficacies of reducing phlegm and relieving cough, and benefiting qi and relieving asthma. The pummelo peel product prepared by the invention can be used independently in one way, has strong efficacies of reducing phlegm and relieving cough, and benefiting qi and relieving asthma, has small effect, does not need compatibility of traditional Chinese medicines with similar efficacies, and belongs to the genuine pummelo peel.

And thirdly, the invention selects a straight circle branch for breeding or uses a seed seedling born by the straight circle branch as the stock, the bud slice is navel bottom shaped orange or golden bell shaped orange bred by scion bud grafting, and the invention has the characteristic of 'one long', namely, the longest sharp thorn is generated on the scion bud section, the original wild nature is sufficient, and the invention has the characteristics of high grease, high fragrance, high bitter taste, high pungent taste and high drug effect 'five high'. The peel is bitter and pungent, the bitter da Xin is strong in smell, and compared with other types of pummelo peel and breeding methods, the pummelo peel can obviously improve the effects of resolving phlegm, relieving cough, activating qi and relieving asthma of the pummelo peel.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be further clearly and completely described below with reference to the embodiments of the present invention. It should be noted that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.

In the embodiment of the present invention, the separation and purification of the organic solvent is performed by a conventional technique, and the present invention is not particularly limited.

The navel-shaped pummelo peel, golden bell-shaped pummelo peel, li Guyuan flat goods, golden stripes and the like used by the invention Chen Guyuan line, liu Fu phoenix tail, tea can fruit, american hair,

The human oranges and the periwool are all from about 60 square kilometers of ilmenite (associated with Qingqing stone ore) in Pingzhong Mingzhou town of pummelo core production area, wherein navel base shape, gold bell shape, money pattern and Western upright wool pummelo varieties are from own orchards of the national pummelo limited of Chua of pummelo city of the company.

Example 1

A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 20 deg.C, and grinding into powder; the pummelo peel is a navel-bottom pummelo peel which is harvested in 12 months in winter, seed seedlings of a straight circle of branches which propagate pummelo fruit trees are rosewood, and bud pieces are scion buds.

Example 2

A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 30 deg.C, and grinding into powder; the pummelo peel is navel-bottom pummelo peel harvested in 12 months in winter, seed seedlings of a straight circle of branches which propagate pummelo peel fruit trees are stocks, and bud pieces are scion buds.

Example 3

A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 40 deg.C, and grinding into powder; the pummelo peel is a navel-bottom pummelo peel which is harvested in 12 months in winter, seed seedlings of a straight circle of branches which propagate pummelo fruit trees are rosewood, and bud pieces are scion buds.

Example 4

A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 45 deg.C, and grinding into powder; the pummelo peel is a navel-bottom pummelo peel which is harvested in 12 months in winter, seed seedlings of a straight circle of branches which propagate pummelo fruit trees are rosewood, and bud pieces are scion buds.

Example 5

A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 45 deg.C, and grinding into powder; the pummelo peel is a navel bottom pummelo peel harvested at the same time as in example 1 and bred by branch propagation in history.

Example 6

A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 45 deg.C, and grinding into powder; the pummelo peel is navel-bottom pummelo peel harvested in 3 months in spring (the last year of pummelo peel), seed seedlings of a round-stem bearing pummelo fruit tree are stock stocks, and bud sheets are scion buds.

Example 7

A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying and grinding exocarpium Citri Grandis at 45 deg.C, extracting the ground exocarpium Citri Grandis powder with water for 1-3 times, mixing water extractive solutions, and concentrating to obtain extract; the pummelo peel is navel bottom type pummelo peel which is harvested at the same time as in the embodiment 1, seed seedlings of a straight circle of branches which carry pummelo fruit trees are stock stocks, and bud pieces are scion buds.

Example 8

A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all flesh, peeling exocarpium Citri Grandis, oven drying and grinding exocarpium Citri Grandis at 45 deg.C, extracting the ground exocarpium Citri Grandis powder with water for 3 times, mixing water extractive solutions, and separating and purifying with organic solvent; the pummelo peel is navel bottom type pummelo peel which is harvested at the same time as in the embodiment 1, seed seedlings of a straight circle of branches which carry pummelo fruit trees are stock stocks, and bud pieces are scion buds.

Example 9

A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 45 deg.C, and grinding into powder; the pummelo peel is golden bell-shaped pummelo peel which is harvested at the same time as in example 5 and has been bred by branch propagation in history.

Comparative examples 1 to 5

A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, drying and grinding; the pummelo peel is navel bottom type pummelo peel harvested at the same time as in the embodiment 1, wherein a seed seedling of a straight circle of branch bearing pummelo fruit trees is a stock and a bud piece is a scion for bud grafting breeding. The drying temperatures of comparative examples 1 to 5 were 50 ℃, 60 ℃, 70 ℃, 80 ℃ and 90 ℃, respectively.

Comparative examples 6 to 8

A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by washing exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 45 deg.C, and grinding into powder. The pummelo peels of the comparative examples 6 to 10 are navel-bottom pummelo peels which are harvested in spring for 3 months, summer for 5 months and autumn for 9 months, bred by long-circle branches to inherit the seed seedlings of pummelo trees as stock stocks and bred by scion buds as bud sheets.

Comparative examples 9 to 17

A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 45 deg.C, and grinding into powder; comparative examples 9 to 17 are Li Guyuan flat goods, money grain, chen Guyuan linear grain, liu Fu goods, phoenix tails, tea cans, western hair, collected at the same time as in example 5 and historically bred in a round-robin fashion,

Human citrus and Fumao.

Comparative example 18

A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 45 deg.C, and grinding into powder; the pummelo peel is harvested at the same time as that in the embodiment 1, shatian pomelo seedlings are used as stock stocks, and bud pieces of pure twigs breeding pummelo peel trees are used as scion and bud grafting breeding pummelo peel.

1. Comparison of different treatment temperatures

1. The dried pummelo peel products prepared in examples 1 to 5 and comparative examples 1 to 5 were compared for aroma, pungent and narcotic taste, squeak and squeak duration. Wherein the squeak is the sound generated during the drying process, and the duration of the squeak is the duration of the sound during the drying process. The test results are shown in table 1.

TABLE 1

As can be seen from table 1, when the drying temperature is higher than 45 ℃, the stronger the aroma and the more intense the aroma, but the tongue feeling of pungent and narcotic taste is not lasting, and the intensity of the substantial reflection is weak. When the drying temperature is 50-90 ℃, the squeak sound appears, which indicates that grease substances in the exocarpium citri grandis peel are pressed by high temperature in the drying process, molecules are displaced and changed, and grease cracks, so the squeak sound is caused. And the drying temperature is 20-40 ℃ without squeak, which shows that the grease substance has stable structure in the temperature range and is more beneficial to maintaining the quality of the drug property.

2. The pummelo peel products prepared by drying the pummelo peel products obtained in the examples 1 to 5 and the comparative examples 1 to 5 are respectively stored for 1 year, 2 years and 3 years at normal temperature. Respectively drying the preserved exocarpium Citri Grandis products at 65 deg.C for 1 hr, and comparing the fragrance and tongue-feeling pungent and anesthetic taste of different exocarpium Citri Grandis products. The results are shown in Table 2.

TABLE 2

As shown in Table 2, the exocarpium Citri Grandis products prepared by the low temperature drying of examples 1-5 were stored for 1 year, 2 years, 3 years, and then dried again at the high temperature of 65 deg.C, which revealed that the low temperature drying at a temperature of not higher than 45 deg.C could burst out a thick and thick fragrance and maintain a strong pungent and spicy anesthetic taste, which indicates that the aromatic and spicy oil substances could be kept stable. And after the comparative examples 1-5 are dried and stored at high temperature, the orange is dried again at high temperature, but the fragrance is light, and the spicy and narcotic taste is weakened, which shows that the oxidation, variation and loss of fragrant and spicy grease substances can be caused after the orange products dried at high temperature are placed for a long time. Therefore, when the efficacy of the pummelo peel product is judged to be one-sided only by the fragrance after primary drying, the fragrance of the pummelo peel product after high-temperature drying is high and cannot reflect the efficacy.

3. The pummelo peel products prepared in the example 2, the comparative example 2 and the comparative example 4 are respectively stored for 1 year, 2 years and 3 years, and then are respectively ground and mixed with warm boiled water according to the mass ratio of 1:10 can be made into paste for treating chronic bronchitis in acute attack stage and cough due to wind-heat.

The treatment method comprises the following steps: the symptoms of the patients with chronic bronchitis of 50-70 years old and cough due to wind-heat are divided into three groups. The patient diagnostic criteria were: (1) Cough, expectoration or asthma, and attack each year for 3 months for 2 or more years; (2) The disease is less than 3 months every year, but clear objective examination basis (such as X-ray, respiratory function determination and the like) exists; (3) And for patients with other heart and lung diseases (such as pulmonary tuberculosis, asthma, bronchiectasis, lung cancer, heart disease, etc.). The acute stage of chronic bronchitis is characterized by: (1) The disease condition suddenly worsens within one week, the cough and the expectoration are aggravated, the sputum quantity is increased, and the sputum is converted into purulent or mucoid sputum or accompanied with other inflammatory manifestations; (2) any one symptom worsens to a severe degree within one week; (3) or severe patients have markedly increased symptoms.

The administration mode is as follows: grinding different prepared pummelo peel products, and mixing the ground pummelo peel products with warm boiled water according to a mass ratio of 1:10 made into paste, and swallowed 1 time.

Effective amount: the administration mode can relieve one of the three characteristic symptoms in the acute attack stage of chronic bronchitis and maintain the relieving effect for more than 4 hours;

judging whether the wind-heat cough is applicable: in the above administration, any one or more symptoms of phlegm, cough, asthma and wind-heat aggravate or are not relieved, which is not applicable, but is applicable on the contrary.

The therapeutic contrast effect is shown in table 3.

TABLE 3

As shown in Table 3, for the treatment of chronic bronchitis, the exocarpium Citri Grandis product prepared in example 2 has the lowest effect, and has therapeutic effect when 1g exocarpium Citri Grandis powder (equivalent to 1/10 of the exocarpium Citri Grandis of 4 double mature fruits) is taken, and the effect of the exocarpium Citri Grandis product is not changed significantly within 3 years, and the exocarpium Citri Grandis product has good effect, and is suitable for the treatment of cough due to pathogenic wind-heat. The comparative examples 2 and 4 have relatively high onset of action, and the dosage changes obviously along with the longer storage time, which shows that the exocarpium citri grandis product prepared by the method has impaired efficacy and reduced drug effect, and is not suitable for treating wind-heat cough.

2. Comparison of different harvest periods

The aroma, bitterness, pungent and anesthetic taste, squeak, and squeak duration of the pummelo peel products prepared in example 4, example 6, and comparative examples 6 to 8 were compared. Wherein the aroma, bitterness and pungent odor are directly judged, and the squeak and squeak duration are the sound and duration of the sound produced by heating the exocarpium Citri Grandis product at 80 deg.C.

And the pummelo peel products prepared in example 4, example 6 and comparative examples 6-8 are mixed with warm boiled water according to the mass ratio of 1.

The treatment method, administration mode and effect amount are the same as above, except that the patients in the test are divided into 5 groups, and the patients are only old chronic bronchitis in acute stage. The results are shown in Table 4.

TABLE 4

As can be seen from table 4, the citrus reticulata blanco obtained in examples 4 and 6 in the maturation stage has stronger aromatic flavor and pungent and anesthetic flavor, stronger squeak sound and longer duration, compared to comparative examples 6 to 8, indicating that more pungent and warm oil substances are contained in the citrus reticulata blanco obtained in the maturation stage. And the effective dose of the pummelo peel products of the examples 4 and 6 is low, which shows that the drug effect is better. When the pummelo peel product is used for treating patients with acute episode of chronic bronchitis, the pummelo peel product collected and prepared in the maturation stage has the highest efficacy, and when the pummelo peel product in other stages is used independently, the comparative examples 6 and 7 cannot cope with the chronic bronchitis disease, and the comparative example 8 needs more onset of action and has poorer efficacy.

3. Comparison of different selected species

1. The citrus red products prepared in example 5, example 9 and comparative examples 9 to 17 were evaluated for comparison of aroma, bitterness and pungent and anesthetic taste. The prepared different pummelo peel products are mixed with warm boiled water according to the mass ratio of 1.

The treatment method, administration mode and effect amount are the same as above, except that the patients in the test are divided into 11 groups, and the patients are only old chronic bronchitis in acute stage.

The results are shown in Table 5.

TABLE 5

As can be seen from Table 5, the umbilical base shape and golden bell shape of the long circle of branches borne breeding have the best effect quality, the outer skin is bitter and pungent, and the big bitter da Xin has the characteristics of five high, namely high fragrance, high bitter taste, high pungent taste, high oil content and high overall effect. In addition, the navel-bottom-shaped pummelo peel and the golden bell-shaped pummelo peel have the characteristic of being long, namely the longest spine is grown on the bud section of the scion, and the spines of other varieties are short or none, which indicates that the navel-bottom-shaped pummelo peel and the golden bell-shaped pummelo peel have enough original wild characteristics and have great significance for the seed selection and breeding of the pummelo peel.

2. The fragrance, bitterness, pungent and anesthetic taste and squeak voice of the pummelo peel products prepared in example 4, example 5 and comparative example 18 were evaluated in comparison. The prepared different pummelo peel products are mixed with warm boiled water according to the mass ratio of 1.

The treatment, mode of administration and amount of effect were the same as above, except that the patients in this test were only patients in the acute episode of chronic bronchitis. The results are shown in Table 6.

TABLE 6

As can be seen from table 6, the property quality of pummelo peel can be maintained only by maintaining a straight circle branch propagation breeding mode or using a straight circle branch propagation seed seedling as stock and using a bud piece as scion bud grafting pure gene for breeding.

4. Can be used for treating chronic bronchitis

270 patients with chronic bronchitis of 50-70 years old (the proportion of male and female is 1:1) are collected, the patients are randomly divided into 9 groups, and 30 patients (15 patients of male and female) in each group are respectively tried with the pummelo peel products prepared in the first to sixth embodiments of the invention.

Wherein the diagnostic criteria are: (1) Cough, expectoration or asthma, with onset of 3 months per year for 2 years or more; (2) The disease is less than 3 months per year, and the patients with definite objective examination basis (such as X-ray, respiratory function determination, etc.) can also be diagnosed; (3) Can be used for treating other heart and lung diseases (such as pulmonary tuberculosis, asthma, bronchiectasis, lung cancer, heart disease, etc.).

The administration mode comprises the following steps: for each example, 1g of orange peel powder was taken and mixed with warm boiled water at a ratio of 1:10, preparing the mixture into paste, and then swallowing the paste or directly taking 2g of extract; the preparation is administered 3 times a day for 10 days as a treatment course, and three treatment courses are provided.

The judgment standard of the curative effect condition is as follows:

and (3) healing: cough, phlegm, asthma, inflammation and other main symptoms are basically controlled, the disease condition is stable, and no recurrence occurs. The effect is shown: the disease condition is relatively stable, the attack frequency and degree are obviously reduced, and the general conditions (including diet, sleep, physical strength and the like) are obviously improved. The method has the following advantages: the disease condition is less stable, but the frequency and degree of attack are reduced, and the general condition is better. And (4) invalidation: the number, degree and general condition of attacks are not improved or aggravated.

Specific treatment outcome profiles are shown in table 7 below:

TABLE 7 therapeutic effects on patients with chronic bronchitis

Group of	Recovery method	Show effect	Is effective	Invalidation
					Example 1	16	10	4	0
Example 2	17	11	2	0
					Example 3	16	11	3	0
Example 4	16	10	4	0
					Example 5	18	12	0	0
Example 6	16	10	4	0
					Example 7	18	7	5	0
Example 8	20	8	2	0
					Example 9	16	14	0	0

Toxicity test

Selecting 90 mice (45 mice each for male and female) with the weight of 18-22 g, dividing the mice into 9 groups, and taking 5 mice each for male and female, wherein the exocarpium citri grandis products prepared in the embodiments 1-9 of the invention and warm boiled water are mixed according to the mass ratio of 1:10 formula of paste, the dosage is 25g/kg, once a day for 10 days. During the period of eating and 1 week after the cessation of eating, the growth state and active diet of the white mouse were observed to identify hematology, blood biochemistry, organ tissue structure, and urinary system. The result shows that all mice survive healthily without any toxic and side effect; the hemogram, liver function and organ tissue state are observed in an anatomical way, and the difference is avoided compared with a normal white mouse. Experiments prove that the pummelo peel product provided by the invention is nontoxic and safe and reliable to eat.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that various changes and modifications can be made by those skilled in the art without departing from the spirit of the invention, and these changes and modifications are all within the scope of the invention. Therefore, the protection scope of the present patent should be subject to the appended claims.

Claims

1. A preparation method of a pummelo peel product is characterized in that the exocarp of pummelo peel is treated at a low temperature, and the temperature of the whole treatment process is 20-45 ℃; the pummelo peel is harvested in the mature period; the harvesting in the maturation stage is exocarpium Citri Grandis harvested in winter every year or exocarpium Citri Grandis harvested in the next spring for the last year; the specific method comprises cleaning exocarpium Citri Grandis with cold water, removing all flesh, peeling outer pericarp, drying and grinding; the pummelo peel is a superior pure pummelo peel.

2. The method of claim 1, wherein the drying is selected from air drying, sun drying, oven drying, and air drying.

3. The method of claim 1, wherein the exocarpium Citri Grandis powder is extracted with water 1~3 times, and the water extracts are combined and concentrated to extract.

4. The method of claim 1, wherein the form of the product is selected from the group consisting of powder, paste, and capsule.

5. The method for preparing a exocarpium Citri Grandis product according to claim 1, wherein the superior product is exocarpium Citri Grandis with navel bottom or exocarpium Citri Grandis with golden bell shape; the pure species is pummelo peel which is bred by bearing through a round of branches in history or by taking seed seedlings of fruit trees bearing through a round of branches as stock stocks and taking bud pieces as scions for bud grafting.

6. A pummelo peel product, characterized in that it is produced by the method according to any of claims 1~5.

7. A Chinese medicinal preparation, comprising the exocarpium Citri Grandis product of claim 6.