CN113940963A - Exocarpium citri grandis product and preparation method and application thereof - Google Patents
Exocarpium citri grandis product and preparation method and application thereof Download PDFInfo
- Publication number
- CN113940963A CN113940963A CN202111238743.9A CN202111238743A CN113940963A CN 113940963 A CN113940963 A CN 113940963A CN 202111238743 A CN202111238743 A CN 202111238743A CN 113940963 A CN113940963 A CN 113940963A
- Authority
- CN
- China
- Prior art keywords
- exocarpium citri
- citri grandis
- pummelo peel
- product
- pummelo
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/06—Antiasthmatics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/10—Expectorants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/14—Antitussive agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Pulmonology (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention relates to the technical field of traditional Chinese medicines, and in particular relates to a pummelo peel product and a preparation method and application thereof. The preparation method of the exocarpium Citri Grandis product comprises treating exocarpium Citri Grandis at low temperature, wherein the temperature of the whole treatment process is not more than 45 deg.C. The invention adopts a low-temperature treatment process, the temperature of the treatment process is controlled not to exceed 45 ℃ in the whole process, so that the lipid substance in the pummelo peel has stable structure and stronger medicinal efficacy of reducing phlegm, relieving cough, benefiting qi and relieving asthma. The prepared exocarpium Citri Grandis product has certain fragrance and persistent pungent and anesthetic taste, and after long-term storage, the fragrance and pungent and anesthetic taste of exocarpium Citri Grandis are not reduced.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicines, and in particular relates to a pummelo peel product and a preparation method and application thereof.
Background
Exocarpium Citri Grandis, also known as exocarpium Citri Grandis and exocarpium Citri Grandis. The tea is warm, bitter and pungent in nature, contains various chemical components such as alkaloid, flavonoid, polysaccharide and volatile oil, has the effects of reducing phlegm and relieving cough, regulating qi and relieving asthma, resolving food stagnation and promoting digestion and the like, and has unique curative effects on treating diseases such as cold cough, chronic cough, bronchitis, pharyngolaryngitis, asthma, food stagnation and alcohol injury. Ming dynasty's famous book Ben Cao gang mu of pharmacy advocates it as "good orange peel product" which has red and white pulp with the efficacy of benefiting qi, resolving phlegm and relieving cough which is more than that of other drugs, … … is good for its effect. Qing dynasty also had the reputations of "true, one-golden and" southern ginseng ", which are listed as palace tributes.
The method comprises collecting exocarpium Citri Grandis in summer when the fruit is immature, slightly scalding in boiling water, cutting pericarp into 5 or 7 pieces, removing fruit pulp and part of mesocarp, press-forming, and drying. However, the Chinese pharmacopoeia only takes the naringin content as a basis, neglects the important function of the pungent-warm volatile oil and oil substances, and the citrus grandis prepared by the provided method has poor drug effect, mediocre phlegm-resolving and cough-relieving efficacy, mediocre qi-benefiting and asthma-relieving efficacy, and other traditional Chinese medicines for resolving phlegm-resolving and cough-relieving and qi-benefiting and asthma-relieving need to be added to enhance the efficacy, so that the chronic bronchitis disease and the chronic obstructive pulmonary disease cannot be treated better. The Chinese invention patent CN105726723A discloses a pummelo peel decoction piece and a processing method thereof, wherein parameters influencing the final finished product quality of the pummelo peel decoction piece, such as drying temperature, time, enzyme deactivation time, cutting size and the like in the processing method of the pummelo peel decoction piece are optimally selected, so that the pummelo peel decoction piece with attractive and uniform appearance and high effective components is obtained. However, the method focuses on the production cycle and the flavor evaluation of the pummelo peel drink, only relatively increases the contents of the oleandoside and the limonene in the sample, and cannot ensure the medicinal efficacy of the pummelo peel.
Therefore, the preparation method standards of the pummelo peel in the current market are not uniform, the quality difference of products with various specifications is large, and the efficacy of the pummelo peel or pummelo peel products circulated in the market is mediocre and can not be used greatly, so that the real value of the pummelo peel is difficult to be exerted.
Disclosure of Invention
In view of the above, there is a need to provide a pummelo peel product, and a preparation method and application thereof. The drug property effect of the pummelo peel is improved by regulating and controlling the collection period of the pummelo peel, the screening and cultivation of varieties, the preparation process and other influencing factors.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the invention provides a preparation method of a pummelo peel product, which is to treat the outer peel of pummelo peel at a low temperature, wherein the temperature of the low temperature is not more than 45 ℃ in the whole treatment process.
Further, in the preparation method of the exocarpium citri grandis product, the specific mode is that the exocarpium citri grandis is washed by cold water, all flesh is removed, outer peel is peeled off, and the outer peel is dried and ground into powder.
Further, in the above method for preparing a exocarpium Citri Grandis product, the drying manner includes, but is not limited to, airing, sun drying, oven drying or air drying.
Further, in the above method for producing a exocarpium Citri Grandis product, the fineness of the milled powder is 200 mesh or more. The exocarpium citri grandis product prepared by the preparation method disclosed by the invention is high in oil content, the powder is in a small lump shape, and if the content is lower than 200 meshes, manual sieving is difficult.
Further, in the preparation method of the pummelo peel product, pummelo peel powder is extracted by water for 1-3 times, and water extracting solutions are combined and concentrated into extraction paste.
Further, in the preparation method of the pummelo peel product, the pummelo peel powder obtained by grinding is extracted by water for 1-3 times, and the water extract is combined and then separated and purified by an organic solvent.
Further, in the preparation method of the pummelo peel product, the pummelo peel is harvested in a mature period.
Further, in the above method for producing a pummelo peel product, the harvesting in the mature period is pummelo peel harvested in winter every year or pummelo peel harvested in spring next year.
Further, in the above preparation method of the pummelo peel product, the pummelo peel is a superior pure pummelo peel; the superior product is exocarpium Citri Grandis with navel bottom or exocarpium Citri Grandis with golden bell shape; the pure breed is pure breed gene exocarpium citri grandis which is bred by always bearing the branches in history or by taking the seed seedlings of the exocarpium citri grandis which are bred by always bearing the branches as stocks and taking bud pieces as scion buds. The original wild property of the high-quality pure pummelo peel is sufficient, and the scion bud joint has the characteristic of producing the longest spine.
In a second aspect, the invention provides a pummelo peel product which is prepared by adopting the preparation method.
Further, in the above-mentioned exocarpium Citri Grandis product, the product form includes, but is not limited to, powder, granule, paste, tablet or capsule.
In a third aspect, the invention provides a traditional Chinese medicine preparation, which comprises the pummelo peel product.
Further, the traditional Chinese medicine preparation also comprises other traditional Chinese medicines and auxiliary materials, wherein the other traditional Chinese medicines include but are not limited to astragalus, angelica, cordyceps sinensis, adenophora stricta, honeysuckle and chrysanthemum.
In a fourth aspect, the invention provides a preparation method of the pummelo peel product, the pummelo peel product and application of a traditional Chinese medicine preparation in preparation of medicines for treating respiratory diseases.
The invention has the beneficial effects that:
compared with the processing mode of processing the whole fruits or the claw pieces of the pummelo peel by using a fire drying or electric drying mode at 50-90 ℃ in the prior art, the invention adopts a low-temperature processing technology, controls the temperature of the processing technology to be not more than 45 ℃ in the whole process, and ensures that the grease substance in the pummelo peel has stable structure and stronger drug property and efficacy. The prepared exocarpium Citri Grandis product has certain fragrance and persistent pungent and anesthetic taste, and after 3 years of long-term storage, the fragrance and pungent and anesthetic taste of exocarpium Citri Grandis are not reduced. Also avoids the defect that the high temperature changes the warm property into the dry heat property, and is no longer suitable for wind-heat cough, and is beneficial to the initial treatment of acute bronchitis.
In the prior art, the non-mature exocarpium citri rubrum picked in summer takes the naringin content as the picking basis, and the important function of obtaining more pungent-warm volatile oil and fat substances can be obtained only by neglecting the mature period. The invention adopts the pummelo peel in the mature period, can greatly improve the aromaticity in the epicarp, and the pungent and warm substances of the grease and the volatile oil, comprehensively and evenly improve the temperature, the bitter and the pungent properties, thicken the unique smell of the pummelo peel, and further improve the efficacies of reducing phlegm and relieving cough, and activating qi and relieving asthma. The pummelo peel product prepared by the invention can be used independently in one way, has strong efficacies of reducing phlegm and relieving cough, and benefiting qi and relieving asthma, has small effect, does not need compatibility of traditional Chinese medicines with similar efficacies, and belongs to the genuine pummelo peel.
And thirdly, the invention selects a straight circle branch for breeding or uses a seed seedling born by the straight circle branch as the stock, the bud slice is navel bottom shaped orange or golden bell shaped orange bred by scion bud grafting, and the invention has the characteristic of 'one long', namely, the longest sharp thorn is generated on the scion bud section, the original wild nature is sufficient, and the invention has the characteristics of high grease, high fragrance, high bitter taste, high pungent taste and high drug effect 'five high'. The peel is bitter and pungent, the bitter is large, the smell is strong, and compared with other types of pummelo peel and breeding methods, the pummelo peel tea can obviously improve the effects of resolving phlegm, relieving cough, activating qi and relieving asthma of the pummelo peel.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be further clearly and completely described below with reference to the embodiments of the present invention. It should be noted that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the embodiment of the present invention, the separation and purification of the organic solvent is performed by a conventional technique, and the present invention is not particularly limited.
Pummelo peel with navel bottom, pummelo peel with golden bell shape, plum garden flat goods, money grain, Chen garden line grain, Lufu goods, phoenix tail, tea can fruit, western hair, red hair, yellow hair, black,
The human oranges and the periwool are all from about 60 square kilometers of ilmenite (associated with Qing stone ore) mining area in Pingzhou town of Huazhou, wherein navel base shape, golden bell shape, golden pattern and Western upright wool orange varieties are from own orchard of Chua garden orange Huazhou Hua Huazhou.
Example 1
A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 20 deg.C, and grinding into powder; the pummelo peel is a navel-bottom pummelo peel which is harvested in 12 months in winter, seed seedlings of a straight circle of branches which propagate pummelo fruit trees are rosewood, and bud pieces are scion buds.
Example 2
A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 30 deg.C, and grinding into powder; the pummelo peel is a navel-bottom pummelo peel which is harvested in 12 months in winter, seed seedlings of a straight circle of branches which propagate pummelo fruit trees are rosewood, and bud pieces are scion buds.
Example 3
A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 40 deg.C, and grinding into powder; the pummelo peel is a navel-bottom pummelo peel which is harvested in 12 months in winter, seed seedlings of a straight circle of branches which propagate pummelo fruit trees are rosewood, and bud pieces are scion buds.
Example 4
A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 45 deg.C, and grinding into powder; the pummelo peel is a navel-bottom pummelo peel which is harvested in 12 months in winter, seed seedlings of a straight circle of branches which propagate pummelo fruit trees are rosewood, and bud pieces are scion buds.
Example 5
A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 45 deg.C, and grinding into powder; the pummelo peel is a navel bottom pummelo peel harvested at the same time as in example 1 and bred by branch propagation in history.
Example 6
A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 45 deg.C, and grinding into powder; the pummelo peel is a navel-bottom pummelo peel which is harvested in 3 months in spring (the pummelo peel of the last year), seed seedlings of a circle of branches bearing pummelo fruit trees are stock stocks, and bud pieces are scion buds for breeding.
Example 7
A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying and grinding exocarpium Citri Grandis at 45 deg.C, extracting the ground exocarpium Citri Grandis powder with water for 1-3 times, mixing water extractive solutions, and concentrating to obtain extract; the pummelo peel is navel bottom type pummelo peel which is harvested at the same time as in the embodiment 1, seed seedlings of a straight circle of branches which carry pummelo fruit trees are stock stocks, and bud pieces are scion buds.
Example 8
A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying and grinding exocarpium Citri Grandis at 45 deg.C, extracting the ground exocarpium Citri Grandis powder with water for 3 times, mixing water extractive solutions, and separating and purifying with organic solvent; the pummelo peel is navel bottom type pummelo peel which is harvested at the same time as in the embodiment 1, seed seedlings of a straight circle of branches which carry pummelo fruit trees are stock stocks, and bud pieces are scion buds.
Example 9
A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 45 deg.C, and grinding into powder; the pummelo peel is golden bell-shaped pummelo peel which is harvested at the same time as in example 5 and has been bred by branch propagation in history.
Comparative examples 1 to 5
A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, drying and grinding; the pummelo peel is navel bottom type pummelo peel harvested at the same time as in the embodiment 1, wherein a seed seedling of a straight circle of branch bearing pummelo fruit trees is a stock and a bud piece is a scion for bud grafting breeding. The drying temperatures of comparative examples 1 to 5 were 50 ℃, 60 ℃, 70 ℃, 80 ℃ and 90 ℃, respectively.
Comparative examples 6 to 8
A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by washing exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 45 deg.C, and grinding into powder. The pummelo peel in the comparative examples 6-10 is navel-bottom pummelo peel which is harvested in spring for 3 months, summer for 5 months and autumn for 9 months, bred by long-circle branches bearing seed seedlings of pummelo fruit trees as stock stocks and bred by scion buds as bud sheets.
Comparative examples 9 to 17
A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 45 deg.C, and grinding into powder; comparative examples 9 to 17 are, respectively, a Japanese plum blossom, a coin blossom, a Chen homeland line, Lufu, phoenix tail, a tea pot fruit, a Western hair, collected at the same time as in example 5 and subjected to a long-term branch-bearing breeding,
Mandarin orange and Fumao.
Comparative example 18
A exocarpium Citri Grandis product is prepared by processing exocarpium Citri Grandis at low temperature by cleaning exocarpium Citri Grandis with cold water, removing all pulp, peeling exocarpium Citri Grandis, oven drying at 45 deg.C, and grinding into powder; the pummelo peel is harvested at the same time as that in the embodiment 1, shatian pomelo seedlings are used as stock stocks, and bud pieces of pure twigs breeding pummelo peel trees are used as scion and bud grafting breeding pummelo peel.
Comparison of one and different processing temperatures
1. The fragrance, spicy and narcotic taste, squeak and squeak duration of the prepared pummelo peel products after drying in examples 1-5 and comparative examples 1-5 were compared. Wherein the squeak is the sound generated during the drying process, and the duration of the squeak is the duration of the sound during the drying process. The test results are shown in table 1.
TABLE 1
As can be seen from table 1, when the drying temperature is higher than 45 ℃, the stronger the aroma and the more intense the aroma, but the tongue feeling of pungent and narcotic taste is not lasting, and the intensity of the substantial reflection is weak. The drying temperature is 50 ~ 90 ℃ the creak sound appears, shows that the grease material receives high temperature oppression in the dry run exocarpium Citri Grandis peel, and the molecule appears shifting, changes, and the grease fracture, therefore causes the creak sound. And the drying temperature is 20-40 ℃ without squeak, which shows that the grease substance has stable structure in the temperature range and is more favorable for maintaining the quality of the drug property.
2. The pummelo peel products prepared in the embodiments 1 to 5 and the comparative examples 1 to 5 are dried and then are respectively stored for 1 year, 2 years and 3 years at normal temperature. Respectively drying the preserved exocarpium Citri Grandis products at 65 deg.C for 1 hr, and comparing the fragrance and granular tongue-feeling pungent and anesthetic taste of different exocarpium Citri Grandis products. The results are shown in Table 2.
TABLE 2
As shown in Table 2, after the pummelo peel products prepared by the low-temperature drying in the examples 1 to 5 are stored for 1 year, 2 years and 3 years, the pummelo peel products are dried again at the high temperature of 65 ℃, and then the pummelo peel products can burst out heavy and thick aroma and keep strong spicy and narcotic flavor, which shows that the low-temperature drying at the temperature of not higher than 45 ℃ can keep the aromatic and spicy grease substances stable. And after the high-temperature drying and storage of the comparative examples 1-5, the pummelo peel is dried again at high temperature, but the fragrance is light, and the spicy and hot anesthetic taste is weakened, which shows that the oxidation, variation and loss of the fragrance and spicy and hot grease substances can be caused after the pummelo peel product dried at high temperature is placed for a long time. Therefore, when the efficacy of the pummelo peel product is judged to be one-sided only by the fragrance after primary drying, the fragrance of the pummelo peel product after high-temperature drying is high and cannot reflect the efficacy.
3. The pummelo peel products prepared in the example 2, the comparative example 2 and the comparative example 4 are respectively stored for 1 year, 2 years and 3 years, and then are respectively ground and mixed with warm boiled water according to the mass ratio of 1:10 can be made into paste for treating chronic bronchitis in acute attack stage and cough due to wind-heat.
The treatment method comprises the following steps: the symptoms of the patients with chronic bronchitis of 50-70 years old and cough due to wind-heat are divided into three groups. The patient diagnostic criteria were: (1) cough, expectoration or asthma, and attack each year for 3 months for 2 or more years; (2) the disease is less than 3 months every year, but there are clear objective examination bases (such as X-ray, respiratory function determination, etc.); (3) and for patients with other heart and lung diseases (such as pulmonary tuberculosis, asthma, bronchiectasis, lung cancer, heart disease, etc.). The acute stage of chronic bronchitis is characterized by: (1) the disease condition suddenly worsens within one week, the cough and the expectoration are aggravated, the sputum quantity is increased, and the sputum is converted into purulent or mucoid sputum or accompanied with other inflammatory manifestations; (2) or any symptom worsened to severe within a week; (3) or the symptoms of critically ill patients are markedly exacerbated.
The administration mode is as follows: grinding different prepared pummelo peel products, and mixing the ground pummelo peel products with warm boiled water according to a mass ratio of 1:10 made into paste, and swallowed 1 time.
Effective amount: the administration mode can relieve one of the three characteristic symptoms in the acute attack stage of chronic bronchitis and maintain the relieving effect for more than 4 hours;
judging whether the wind-heat cough is applicable: in the above administration, any one or more symptoms of phlegm, cough, asthma and wind-heat aggravate or are not relieved, which is not applicable, but is applicable on the contrary.
The therapeutic contrast effect is shown in table 3.
TABLE 3
As can be seen from table 3, for the treatment of chronic bronchitis, the pummelo peel product prepared in example 2 has the lowest effect, and has the treatment effect when 1g of pummelo peel powder (corresponding to 1/10 of peel of 4 double ripe fruits) is taken, and the effect and the dosage are not obviously changed within 3 years, so that the efficacy of the pummelo peel product can be well preserved, and the pummelo peel product is suitable for the treatment of wind-heat cough. The comparative examples 2 and 4 have relatively high onset of action, and the dosage changes obviously along with the longer storage time, which shows that the exocarpium citri grandis product prepared by the method has impaired efficacy and reduced drug effect, and is not suitable for treating wind-heat cough.
Second, comparison of different harvesting periods
The aroma, bitterness, pungent and anesthetic taste, squeak, and squeak duration of the pummelo peel products prepared in example 4, example 6, and comparative examples 6-8 were compared. Wherein the aroma, bitterness and pungent odor are directly judged, and the squeak and squeak duration are the sound and duration of the sound produced by heating the exocarpium Citri Grandis product at 80 deg.C.
And the pummelo peel products prepared in the examples 4, 6 and 6-8 are mixed with warm boiled water according to the mass ratio of 1:10 respectively, and then are respectively used for treating patients with chronic bronchitis in acute attack.
The treatment method, administration mode and effect amount are the same as above, except that the patients in the test are divided into 5 groups, and the patients are only old chronic bronchitis in acute stage. The results are shown in Table 4.
TABLE 4
As can be seen from table 4, compared with comparative examples 6 to 8, the pummelo peels harvested in the maturation stage in examples 4 and 6 have stronger aromatic flavor and pungent and anesthetic flavor, stronger squeak sound and longer duration, which indicates that more pungent and warm oil substances are contained in the pummelo peels harvested in the maturation stage. And the effective dose of the pummelo peel products of the examples 4 and 6 is low, which shows that the drug effect is better. When the pummelo peel product is used for treating patients with acute episode of chronic bronchitis, the pummelo peel product collected and prepared in the maturation stage has the highest efficacy, and when the pummelo peel product in other stages is used independently, the comparative examples 6 and 7 cannot cope with the chronic bronchitis disease, and the comparative example 8 needs more onset of action and has poorer efficacy.
Comparison of three, different species
1. The citrus red products prepared in examples 5 and 9 and comparative examples 9 to 17 were evaluated for comparison of aroma, bitterness, and pungent and narcotic taste. The prepared different pummelo peel products are mixed with warm boiled water according to the mass ratio of 1:10 and are respectively used for treating patients with acute attack of old chronic bronchitis.
The treatment method, administration mode and effect amount are the same as above, except that the patients in the test are divided into 11 groups, and the patients are only old chronic bronchitis in acute stage.
The results are shown in Table 5.
TABLE 5
As can be seen from Table 5, the umbilical base shape and golden bell shape of the long circle of branches inheriting breeding have the best quality of efficacy, the outer skin is bitter and pungent, and the bitter is bitter and pungent, and has the characteristics of five highs, namely high fragrance, high bitter taste, high pungent taste, high oil content and high overall efficacy. In addition, the navel-bottom-shaped pummelo peel and the golden bell-shaped pummelo peel have the characteristic of being long, namely the longest prickle is generated at the bud joint of the scion, and the other varieties have short or no prickles, which indicates that the navel-bottom-shaped pummelo peel and the golden bell-shaped pummelo peel have enough original wild properties and have great significance for the seed selection and breeding of the pummelo peel.
2. The fragrance, bitterness, pungent and anesthetic taste and squeak voice of the pummelo peel products prepared in example 4, example 5 and comparative example 18 were evaluated in comparison. The prepared different pummelo peel products are mixed with warm boiled water according to the mass ratio of 1:10 and are respectively used for treating patients with acute attack of old chronic bronchitis.
The treatment, mode of administration and amount of effect were the same as above, except that the patients in this test were only patients in the acute episode of chronic bronchitis. The results are shown in Table 6.
TABLE 6
As can be seen from table 6, the property quality of pummelo peel can be maintained only by maintaining a straight circle branch propagation breeding mode or using a straight circle branch propagation seed seedling as stock and using a bud piece as scion bud grafting pure gene for breeding.
Fourth, the curative effect for patients with chronic bronchitis
270 chronic bronchitis patients of 50-70 years old (the proportion of male to female is 1:1) are collected, the chronic bronchitis patients are randomly divided into 9 groups, 30 chronic bronchitis patients of each group (15 chronic bronchitis patients of male and female) are subjected to trial application of the pummelo peel products prepared in the first embodiment to the sixth embodiment of the invention.
Wherein the diagnostic criteria are: (1) cough, expectoration or asthma, with onset of 3 months per year for 2 years or more; (2) the disease is less than 3 months per year, and the patients with definite objective examination basis (such as X-ray, respiratory function determination, etc.) can also be diagnosed; (3) can be used for treating other heart and lung diseases (such as pulmonary tuberculosis, asthma, bronchiectasis, lung cancer, heart disease, etc.).
The administration mode comprises the following steps: for each example, 1g of orange peel powder was taken and mixed with warm boiled water at a ratio of 1:10 mass ratio to prepare paste, and then swallow the paste, or directly take 2g of extract; the preparation is administered 3 times a day for 10 days as a treatment course, and three treatment courses are provided.
The judgment standard of the curative effect condition is as follows:
and (3) healing: cough, phlegm, asthma, inflammation and other main symptoms are basically controlled, the disease condition is stable, and no recurrence occurs. The effect is shown: the disease condition is relatively stable, the attack frequency and degree are obviously reduced, and the general conditions (including diet, sleep, physical strength and the like) are obviously improved. The method has the following advantages: the disease condition is less stable, but the frequency and degree of attack are reduced, and the general condition is better. And (4) invalidation: the number, degree and general condition of attacks are not improved or aggravated.
Specific treatment outcome profiles are shown in table 7 below:
TABLE 7 therapeutic effects on patients with chronic bronchitis
| Group of | Recovery method | Show effect | Is effective | Invalidation |
| Example 1 | 16 | 10 | 4 | 0 |
| Example 2 | 17 | 11 | 2 | 0 |
| Example 3 | 16 | 11 | 3 | 0 |
| Example 4 | 16 | 10 | 4 | 0 |
| Example 5 | 18 | 12 | 0 | 0 |
| Example 6 | 16 | 10 | 4 | 0 |
| Example 7 | 18 | 7 | 5 | 0 |
| Example 8 | 20 | 8 | 2 | 0 |
| Example 9 | 16 | 14 | 0 | 0 |
Toxicity test
Selecting 90 mice (45 mice each for male and female) with the weight of 18-22 g, dividing the mice into 9 groups, and taking 5 mice each for male and female, wherein the exocarpium citri grandis products prepared in the embodiments 1-9 of the invention and warm boiled water are mixed according to the mass ratio of 1:10 formula of paste, the dosage is 25g/kg, once a day for 10 days. During the period of eating and 1 week after the cessation of eating, the growth state and active diet of the white mouse were observed to identify hematology, blood biochemistry, organ tissue structure, and urinary system. The result shows that all mice survive healthily without any toxic and side effect; the hemogram, liver function and organ tissue state are observed in an anatomical way, and the difference is avoided compared with a normal white mouse. Experiments prove that the pummelo peel product provided by the invention is nontoxic and safe and reliable to eat.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A preparation method of a pummelo peel product is characterized in that the exocarp of pummelo peel is treated at a low temperature, and the temperature of the whole treatment process at the low temperature is not more than 45 ℃.
2. The method of claim 1, wherein the exocarpium Citri Grandis is prepared by washing exocarpium Citri Grandis with cold water, removing all flesh, peeling outer pericarp, drying outer pericarp, and grinding into powder.
3. The method of claim 1, wherein the drying is selected from the group consisting of air drying, sun drying, oven drying, and air drying.
4. The method for preparing exocarpium Citri Grandis product according to claim 2, wherein exocarpium Citri Grandis powder is extracted with water for 1-3 times, and the water extractive solutions are combined and concentrated to extract.
5. The method for preparing a pummelo peel product according to claim 1, wherein the pummelo peel is a pummelo peel harvested at the mature stage; the harvesting in the mature period is pummelo peel harvested in winter every year or pummelo peel harvested in spring next year in the last year.
6. The method of claim 1, wherein the product form includes but is not limited to powder, paste, tablet or capsule.
7. The method for preparing a pummelo peel product according to claim 1, wherein the pummelo peel is a superior pure pummelo peel; the superior product is exocarpium Citri Grandis with navel bottom or exocarpium Citri Grandis with golden bell shape; the pure species is pummelo peel which is bred by bearing through a round of branches in history or by taking seed seedlings of fruit trees bearing through a round of branches as stock stocks and taking bud pieces as scions for bud grafting.
8. A pummelo peel product, which is prepared by the preparation method of any one of claims 1 to 7.
9. A Chinese medicinal preparation, comprising the exocarpium Citri Grandis product of claim 8.
10. Use of the method of any one of claims 1 to 7 or the exocarpium Citri Grandis product of claim 8 or the Chinese medicinal preparation of claim 9 in the preparation of a medicament for treating respiratory diseases.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111238743.9A CN113940963B (en) | 2021-10-25 | 2021-10-25 | Exocarpium citri grandis product and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111238743.9A CN113940963B (en) | 2021-10-25 | 2021-10-25 | Exocarpium citri grandis product and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113940963A true CN113940963A (en) | 2022-01-18 |
| CN113940963B CN113940963B (en) | 2023-01-13 |
Family
ID=79332078
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111238743.9A Active CN113940963B (en) | 2021-10-25 | 2021-10-25 | Exocarpium citri grandis product and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113940963B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105726723A (en) * | 2016-03-25 | 2016-07-06 | 广州市香雪制药股份有限公司 | Pummelo peel decoction pieces as well as processing method and application thereof |
| CN107647401A (en) * | 2017-09-13 | 2018-02-02 | 化州市蔡家园化橘红有限公司 | Exocarpium Citri Grandis rascal honey slurry and preparation method thereof |
| CN108129532A (en) * | 2018-02-05 | 2018-06-08 | 广东药科大学 | A kind of method that aurantiin is prepared based on Exocarpium Citri Grandis |
| CN108704022A (en) * | 2018-06-20 | 2018-10-26 | 李锋 | A kind of medical, edible Exocarpium Citri Grandis ingredient preparation method and its preparation |
| CN111514206A (en) * | 2020-06-04 | 2020-08-11 | 化州市万添生物科技发展有限公司 | Processing method of bitter-removed pummelo peel |
| WO2021128785A1 (en) * | 2019-12-23 | 2021-07-01 | 深圳市药品检验研究院（深圳市医疗器械检测中心） | Pattern recognition method for evaluating quality of exocarpium citrus grandis, computer device and computer-readable storage medium |
-
2021
- 2021-10-25 CN CN202111238743.9A patent/CN113940963B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105726723A (en) * | 2016-03-25 | 2016-07-06 | 广州市香雪制药股份有限公司 | Pummelo peel decoction pieces as well as processing method and application thereof |
| CN107647401A (en) * | 2017-09-13 | 2018-02-02 | 化州市蔡家园化橘红有限公司 | Exocarpium Citri Grandis rascal honey slurry and preparation method thereof |
| CN108129532A (en) * | 2018-02-05 | 2018-06-08 | 广东药科大学 | A kind of method that aurantiin is prepared based on Exocarpium Citri Grandis |
| CN108704022A (en) * | 2018-06-20 | 2018-10-26 | 李锋 | A kind of medical, edible Exocarpium Citri Grandis ingredient preparation method and its preparation |
| WO2021128785A1 (en) * | 2019-12-23 | 2021-07-01 | 深圳市药品检验研究院（深圳市医疗器械检测中心） | Pattern recognition method for evaluating quality of exocarpium citrus grandis, computer device and computer-readable storage medium |
| CN111514206A (en) * | 2020-06-04 | 2020-08-11 | 化州市万添生物科技发展有限公司 | Processing method of bitter-removed pummelo peel |
Non-Patent Citations (3)
| Title |
|---|
| 周秋丽等: "《现代中药基础研究与临床》", 30 June 2012 * |
| 郭力等: "《中药化学》", 31 August 2018 * |
| 黄燮才等: "《妇科病中草药识别与应用》", 31 December 2017 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113940963B (en) | 2023-01-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR100549286B1 (en) | Manufacturing method for traditional rice wine flavoring with citrus junos | |
| CN103509677A (en) | Melastoma dodecandrum lour health-care fruit wine | |
| CN104673608A (en) | Preparation method for moringa oleifera health wine | |
| Ojimelukwe et al. | The traditional and medicinal use of African breadfruit (Treculia africana Decne): an underutilized ethnic food of the Ibo tribe of South East, Nigeria | |
| Toungos | Tamarind (Tamarindus indicus L) fruit of potential value but underutilized in Nigeria | |
| KR20090098024A (en) | The method of manufacturing of medicinal wine | |
| KR20120134750A (en) | Manufacturing method of medicinal herb hot pepper soy paste | |
| CN105124670B (en) | A kind of health drink and preparation method thereof | |
| CN104643219A (en) | Health blueberry and bitter gourd composite fruit juice beverage and preparation method thereof | |
| CN113940963B (en) | Exocarpium citri grandis product and preparation method and application thereof | |
| KR20110056908A (en) | Composition of mixed herb medicine for leached tea | |
| Chaudhary | A review on the culinary uses and therapeutic properties of Murraya koenigii | |
| KR20170115873A (en) | Fruit and tuber juice and method of making the same | |
| CN103621737B (en) | The processing method of a kind of flavouring dragon palpus gynostemma pentaphylla | |
| KR100768471B1 (en) | Manufacturing method of beverage extract using pine cone and pine cone | |
| KR101535896B1 (en) | Method of preparing eriobotrya japonica tea beverage | |
| KR20110035835A (en) | Making method of jelly comprising ginseng fruit | |
| JP3425918B2 (en) | Processing method of Xianfeng soybean to reduce tannin content | |
| KR20190003876A (en) | The method for manufacturing of health drink | |
| CN107549391A (en) | A kind of preparation method of high chlorogenic acid content blueberry leaf green tea | |
| JP2009518055A (en) | Functional food composition having therapeutic effect on allergy, natural tea using the same, and method for producing the same | |
| KR20200137842A (en) | Manufacturing method of jujube tea and jujube tea manufactured by the same | |
| CN111134260A (en) | Dendrobium officinale plant beverage and preparation method thereof | |
| KR101799815B1 (en) | Manufacturing method of Dolsan mustard Kimchi containing Saposhnikovia divaricata Schiskin and mustard Kimchi thereof | |
| CN107897914A (en) | A kind of a kind of sedge dish nutrient solution with hypolipemic function |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |